Electrohydrodynamically formed core-shell structure of chitosan-starch composite microparticles for induced surface functionality and phased release of bioactive species

Abstract

Microparticles from biopolymers with multiple functionalities are sought after in many controlled release applications. As an example, ultra-porous hemostatic materials with the option of dual delivery of procoagulant and antibacterial agents is highly advantageous for wound management. In this study, uniform microcapsules of core-shell structure at sub-millimeter scale were fabricated by disintegrating electrostatically a flow of a core-sheath filament. The chitosan at the core contained gentamicin as an antibacterial agent, whereas crosslinked starch as the shell was loaded with tranexamic acid (TA) that was meant to serve as blood pro-coagulant. The stretching of core-sheath filament under electrostatic force and subsequent disintegration in a stable cone-jet mode resulted in highly uniform microcapsules of diameter 180 ±20 µm, which apparently was much smaller than the dimension of the nozzle. Characterization of the core–shell structure was performed through scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and nitrogen adsorption–desorption analysis, confirming the distinct compartmentalization and with intrinsic porosity at mesoscale. These properties enabled large uptake of water soluble drug or blood procoagulant, apart from offering a scaffold for absorption of plasma or wound exudate, and providing surface area for adhesion and/or aggregation of platelets. The developed microparticles exhibited a water absorption capacity of 462.3% and underwent 80% biodegradation over an 8-day period, indicating their suitability for wound management. The burst release of TA from the shell (over a timescale of ~ 4 h), and sustained release of gentamicin from the core (over a timescale of ~72 hours were demonstrated.