Shielding against Breast Tumor Relapse with an Autologous Chemo-Photo-Immune active Nano-Micro-Sera based Fibrin Implant

Abstract

Local recurrence post-surgery in early-stage triple-negative breast cancer is a major challenge. To control the regrowth of residual tumor, we have developed an autologous therapeutic hybrid fibrin glue for intra-operative implantation. Using autologous serum proteins as stabilizers, we have optimized high drug-loaded lapatinib-NanoSera (Lap-NS; ~ 66 % L.C), Imiquimod-MicroSera (IMQ-MS; ~ 92 % L.C). Additionally, plasmonic NanoSera (PNS) with ~ 67 % photothermal conversion efficiency under 980 nm laser irradiation was also developed. While localized monotherapy with either Lap-NS or PNS reduced tumor regrowth rate, their combination with IMQ-MS amplified the effect of immunogenic cell death with a high level of tumor infiltration by immune cells at the surgical site. The localized combination immunotherapy with Nano-Micro-Sera based hybrid fibrin implant showed superior tumor inhibition and survival with significant promise for clinical translation.