Reversing the stereoselectivity of intramolecular [2+2] photocycloaddition utilizing cucurbit[8]uril as a molecular flask †

Macrocyclic hosts, such as cucurbit[8]uril (CB8), can significantly influence the outcomes of chemical reactions involving encapsulated reactive guests. In this study, we demonstrate that CB8 completely reverses the stereoselectivity of intramolecular [2+2] photo-cycloaddition reactions. Notably, it was also found that CB8 can trigger the unreactive diene to be reactive.

Reversing the stereoselectivity of intramolecular [2+2] photocycloaddition utilizing cucurbit [8]uril as a molecular flask † Xujun Qiu, a Jasmin Seibert, a Olaf Fuhr, bc Frank Biedermann * b and Stefan Bra ¨se * ad Macrocyclic hosts, such as cucurbit [8]uril (CB8), can significantly influence the outcomes of chemical reactions involving encapsulated reactive guests.In this study, we demonstrate that CB8 completely reverses the stereoselectivity of intramolecular [2+2] photo-cycloaddition reactions.Notably, it was also found that CB8 can trigger the unreactive diene to be reactive.
Recent advancements in carrying out organic reactions within ''molecular flasks'', 5,6 specifically using supramolecular hosts, such as macrocycles, 7-9 cages [10][11][12][13][14] and capsules, [15][16][17] have paved the way for novel reaction pathways.The reactivity and selectivity of reagents is controlled by the microenvironment, e.g., the confined space within the cavity of molecular flasks assists in preorganizing the substrates, 18,19 which modulates the reaction rates or steers reactions in directions distinct from those in bulk solutions.
Cucurbit[n]urils (CBn) are characterized by their symmetrical, rigid, and hydrophobic cavities that can host organic molecules. 20,21Their nanocavities are excellent binders for hydrophobic molecules in aqueous environments, while the carbonyl-fringed portal regions provide an additional driving force for binding cationic guests.][29] In this study, we showcase how the outcome of intramolecular [2+2] photocycloaddition can be switched from an anti-head-to-tail to a syn-head-to-tail cycloaddition product using CB8 as a molecular flask.Furthermore, it was also found that CB8 can trigger the unreactive diene to be reactive, yielding a syn-head-to-head (1,2) product.As depicted in Scheme 1, diene 1 experiences intramolecular charge repulsion due to its two cationic methylpyridinium moieties.We started our investigation with the photolysis of 1 in water, using a standard photoreactor fitted with 14 UVA (365 nm) lamps. 1 H NMR spectra (Fig. S4, ESI †) were recorded at fixed time intervals to monitor the reaction path.
As irradiation progressed, the 1 H peaks from 1 gradually disappeared, while new signals corresponding to a single intramolecular cycloaddition product emerged.After approximately 18 minutes of UV exposure, no further significant changes were observed in the NMR spectra, indicating the completion of the photolysis.Structural assignment of the product peaks revealed that UV irradiation of 1 in water proceeds via photoisomerization and [2+2] cycloaddition, yielding an anti-head-to-tail (1,3) product where the charge repulsion between the pyridinium moieties is minimized (refer to Fig. S7-S15 in the ESI † for a detailed structural assignment of the reaction product.) Intrigued by the potential impact of a confined environment on the photoreaction process, we explored whether the photoisomerization of molecule 1 could be inhibited within the cavity of a molecular host, and whether this altered the outcome of the [2+2] cycloaddition.We selected the water-soluble molecular host CB8 as our choice, given its spacious and hydrophobic cavity, which could accommodate both vinylpyridinium residues of 1 simultaneously.To test this binding hypothesis, we first examined the host-guest properties between 1 and CB8 using 1 H NMR. As depicted in Fig. 2a, when an aqueous solution of 1 and CB8 was mixed in a 1 : 1 ratio in D 2 O, the NMR signals a-d, corresponding to the vinylpyridinium residues, showed significant upfield shifts, indicating that these are bound inside the shielding CB8 cavity.Conversely, the peaks corresponding to the naphthyl moieties (e-g) displayed slight downfield shifts, suggesting that these moieties are located outside or near the rim of the CB8 portal. 30otably, the signals H x and H y from CB8 split into four sets, reflecting the deformation of CB8 when molecule 1 is accommodated within its cavity.The formation of the 1 : 1 complex of 1ÁCB8 was further confirmed by ESI-MS experiments (Fig. S16, ESI †), where a peak with a mass-to-charge ratio (m/z) of 846.2923 for [1ÁCB8-2Cl] 2+ was observed.
We further explored the binding affinity between 1 and CB8 through UV titration, gradually introducing CB8 into a solution of 1.As depicted in Fig. 1b, the addition of CB8 led to a decrease in absorbance, with the two maximum absorbance peaks at 281 nm and 396 nm shifting to isosbestic points 293 nm and 421 nm, respectively.We plotted the changes in absorbance at 281 nm against the concentration of CB8, as shown in Fig. 1c.Least-square fitting of this data with a direct binding assay (DBA) model yielded a binding constant of (2.53 AE 0.11) Â 10 6 M À1 , indicating a strong interaction between 1 and CB8.
Furthermore, we performed the photoreaction of 1 encapsulated in the CB8 cavity under the same photoreaction conditions as for 1 in water for comparison.Upon irradiation, the 1 H NMR signals were observed to be completely different from the initial state, suggesting the formation of new species (Fig. S19, ESI †).After around 21 min, no more significant variation in the NMR suggests the end of the reaction.
A similar phenomenon in UV absorbance and fluorescence emission change was observed (Fig. S20 and S21, ESI †).As shown in Fig. 2b, the absorbance was observed to decrease Scheme 1 Schematic illustration of modulating the photolysis reaction outcome of 1 utilizing cucurbit [8]uril as a molecular flask.dramatically within the first 3 min, in which the maximum absorption peak at 421 nm was eventually barely visible after being irradiated for 21 min.The fluorescence intensity was identical upon UV irradiation, which decreased rapidly to no emission at the end, Fig. 2c.The formation of [2+2] cycloaddition product 3 in the CB8 cavity was also evidenced by ESI-MS (Fig. S24, ESI †), in which a strong signal peak with a mass-tocharge ratio (m/z) of 846.2927 that corresponded to [3ÁCB8-2Cl] 2+ was observed, which suggested no mass change of 3 in the CB8 cavity compared to starting reagent 1.
Upon addition of the strongly CB8-binding competitive guest, memantine hydrochloride, to the 3ÁCB8 complex, unbound 3 was obtained, as shown in Fig. 2a (for full spectra see Fig. S25, ESI †).The symmetric signals in Fig. 2a (Fig. S25,  ESI †) indicate a syn-head-to-tail (1,3) photo cycloaddition product 3, which is different from the similar structure reported in the literature. 31Notably, the binding constant of 3 between CB8 was determined to be (2.04AE 0.29) Â 10 12 M À1 using a competitive binding assay (CBA), where memantine was utilized as a competitive guest (Fig. S42, ESI †). 32The dramatically increased binding constant of 3 compared with starting molecule 1 could result from the rigidified structure of 3 having a better complemental effect with the CB8 void.Further studies were conducted to understand the mechanistic pathway of the reaction.The rate of product formation was found to be significantly influenced by the concentration of CB8, suggesting a pivotal role of the macrocycle in the reaction kinetics.Control experiments, conducted in the absence of CB8 or in the presence of smaller macrocycle CB7 that cannot fully encapsulate 1 in its cavity due to sterical reasons, further corroborated its role in steering the reaction towards the synhead-to-tail (1,3) cycloaddition product.In the meantime, controlled experiments in the dark under thermal conditions were performed for 1 and 1ÁCB8, and no reaction traces were observed for both cases.To gain more insights into the stereo structures of the different possible products, we performed theoretical calculations and predicted the NMR composition (Table S1, ESI †), which further supported our conclusions.
We then wondered whether the photo cycloaddition process could still take place if two olefin branches were covalently locked.Therefore, molecule 4, in which the pyridine moieties were bridged by 1,4-bis(bromomethyl)benzene, was designed, synthesized, and fully characterized (Fig. S27-S29, ESI †).As shown in Fig. 3a and b, the crystal structure suggests that molecule 4 bears a rigid structure and the olefin groups are separated distinctly.Thus, photolysis of molecule 4 in aqueous media results in no change.In the same fashion, we then studied the host-guest interaction between 4 and CB8.Indeed, molecule 4 can be encapsulated within the CB8 cavity, as indicated by the ESI-MS (Fig. S30, ESI †), and NMR spectra (Fig. S31, ESI †).The NMR spectra of 4 and 4ÁCB8 suggest that CB8 selectively encapsulates a naphthyl moiety, while the bridged pyridinium locates at the carbonyl portal of the CB8, which is comparable to the literature. 33The binding constant  Surprisingly, upon the irradiation of 4ÁCB8 under the same reaction conditions as photolysis of 1 and 1ÁCB8, new species were observed in the NMR spectra (Fig. 3c and Fig. S34, ESI †).Absorption (Fig. S35, ESI †) and emission spectra (Fig. S36, ESI †) also revealed the occurrence of the photoreaction.The mass spectra (Fig. S37, ESI †) of the 5ÁCB8 complex suggest no mass change of the photoreaction product.The NMR spectra (Fig. 3c and Fig. S38, ESI †) indicate that a dissymmetric product is formed, and the identical signal at 5.18-5.27ppm suggests that the syn-head-to-head (1,2) cycloaddition product is generated, which is comparable to the similar structure. 31To obtain unbound product 5 out of the CB8 cavity, memantine hydrochloride was introduced to the reaction mixture (Fig. 3c and Fig. S40, ESI †).
In conclusion, we presented a strategy to selectively tune the photoreaction pathway utilizing CB8 as a molecular flask.The photoreaction of molecule 1 in bulk water was assisted with the photoisomerization and [2+2] cycloaddition process, resulting in an anti-head-to-tail (1,3) photoproduct 2. However, with the template from CB8, the photoreaction selectively formed syn-head-to-tail (1,3) cycloaddition product 3. Notably, the binding constant between 3 and CB8 was estimated to be as high as (2.04 AE 0.29) Â 10 12 M À1 .When molecule 4 was intramolecularly locked, photoreaction was prevented upon irradiation.On the contrary, when the photoreaction was performed inside the CB8 cavity, a syn-head-to-head (1,2) product was obtained.