A pillar[5]arene-based planar chiral charge-transfer dye with enhanced circularly polarized luminescence and multiple responsive chiroptical changes

The fabrication of circularly polarized luminescent (CPL) organic dyes based on macrocyclic architecture has become an importantly studied topic in recent years because it is of great importance to both chiral science and supramolecular chemistry, where pillar[n]arenes are emerging as a promising class of planar chiral macrocyclic hosts for CPL. We herein synthesized an unusual planar chiral charge-transfer dye (P5BB) by covalent coupling of triarylborane (Ar3B) as an electron acceptor to parent pillar[5]arene as an electron donor. The intramolecular charge transfer (ICT) nature of P5BB not only caused a thermally responsive emission but also boosted the luminescence dissymmetry factor (glum). Interestingly, the specific binding of fluoride ions changed the photophysical properties of P5BB, including absorption, fluorescence, circular dichroism (CD), and CPL, which could be exploited as an optical probe for multi-channel detection of fluoride ions. Furthermore, the chiroptical changes were observed upon addition of 1,4-dibromobutane as an achiral guest.


Introduction
Chirality, as one of the most signicant phenomena, is ubiquitous in life and the environment, and determines the daily physiological activities and metabolism of life. 1 Chiral science unarguably promotes the development of life, medicine and materials science. 2 Chiroptical functional materials with circularly polarized luminescence (CPL) have drawn great attention in the last decade, 3 not only for the understanding of the inherent principles of chirality but also owing to their wide potential applications in chiral sensing, 4 photoelectric devices, 5 3D displays, 6 asymmetric catalysis 7 and so forth. Generally, CPL is generated due to the molecules or supramolecular aggregates having both chiral features and luminescent properties. 8 Therefore, it has become an effective strategy to achieve CPL activity by connecting luminophores with chiral fragments (e.g., binaphthyls, helicenes, and [2.2]paracyclophane). 9 Recently, CPL-active systems based on planar chiral analogues have attracted increasing attention, 10 because their inherent macrocyclic skeletons have more important potential in the eld of chiral supramolecular chemistry (e.g., chiral network gelation and chiral host-guest recognition).
Pillararenes, 11 as an important type of macrocyclic arene, have attracted extensive studies and made signicant contributions in host-guest recognition and self-assembly because of their unique structure and easy synthesis. 12 Pillararenes possess planar chirality, which comes from the different orientations of 1,4alkoxyphenyl units. 13 Although the enantiomers (pS and pR) are easy to interconvert due to the dynamic rotations of phenyl units, stable chiral congurations can still be achieved by reasonable molecular functionalization of the parent pillararenes. For example, introducing 10 cyclohexylmethyl groups at both rims of pillar[5]arene could prevent the rotations of benzene rings. 14 Stoddart et al. also developed an effective strategy to obtain separable enantiomers by introducing bulky p-conjugated units at the A1/A2 positions of pillar[5]arene. 15 Recently, pillararenes have been used to prepare CPL-active molecules by integrating with appropriate uorophores. 16 For example, Chen et al. reported two p-conjugated CPL-active systems (P5NN and P5BN) through axial functionalization of pillar[5]arene with sterically bulky triarylamine (Ar 3 N) and triarylborane (Ar 3 B) (Scheme 1); however, the g lum values were only a 10 −4 order because the luminescence largely depended on axial p-conjugated uorophores and hence limited the transfer of chirality. 17 In 2022, Ogoshi et al. reported a series of rim-differentiated C 5 -symmetric pillar[5]arenes with improved g lum values, but it was difficult to obtain proper uorescence efficiency while improving the asymmetry factor. 18 Therefore, the design and synthesis of CPL-active pillararenes at the molecular level with a good balance between g lum factors and luminescence efficiency are highly anticipated.
Theoretically, the g lum factor is simply approximated using 4jmjcos q/jmj, where m, m and q are the magnetic transition dipole moment, electric transition dipole moment and the angle between m and m, respectively. 19 If organic systems are appropriately designed to have weaker electric dipole transition and stronger magnetic dipole transition, it could lead to CPL-active materials with high g lum . In fact, a large number of chiral organic molecules show relatively low g lum values because of their electric dipole-allowed but magnetic dipole-forbidden transitions. 20 In contrast, because charge transfer (CT) systems possess relatively small jmj and large jmj, the larger g lum values obtained from the CT state are hypothesized. 21 As a typical electron acceptor, Ar 3 B has extensive application prospects in organic optoelectronic materials and stimulus-responsive materials. 22 Notably, Ar 3 B are enabled to show distinctive CT emission once they are electronically coupled with electron donors. 23 Pillararenes possess electron rich macrocyclic structures and can be used as a kind of electron donor. As one of our continuous pursuits of functionalized pillar[5]arenes, 24 we herein propose a facile methodology to amplify g lum values through functionalizing the pillar [5]arene parent to construct planar chiral CT dye (P5BB) with Ar 3 B. Sterically bulky Ar 3 B uorophores not only allow the enantiomeric resolution but also promote intramolecular charge transfer (ICT) from pillar[5]arene to Ar 3 B. Based on the inherent host-guest nature of pillar [5]arene and the stimulus-responses of Ar 3 B, the chiroptical response behaviors of this chiral system were further studied. The details are presented herein.

Results and discussion
The key synthetic process of P5BB is shown in Scheme 2a and the ESI. † The core planar chiral block P5-OTf was directly obtained via the previous report, 25 and then Pd-catalyzed Suzuki coupling by the reaction of P5-OTf with 2.0 equiv. Mes 2 B-containing phenylboronic acid led to the formation of P5BB in 45% yield. Similarly, PhBB was also obtained by standard Suzuki coupling of (4-bromophenyl)dimesitylborane with 1,4-phenylenediboronic acid in 38% yield. The chemical structures of P5BB and PhBB were fully characterized by 1 H, 13 C, and 11 B NMR and high-resolution mass spectrometry (HRMS). A single crystal of rac-P5BB for Xray diffraction analysis was collected by slowly evaporating the solution of acetone/MeOH (v/v = 1 : 1). In the crystalline form, a highly twisted p-conjugated skeleton was observed, as conrmed by dihedral angles (a 1 and a 2 ) that are measured to be 73°and 88°, respectively (Scheme 2b). Molecular size measurement revealed that the Ar 3 B-substituents (d 1 = 11.22Å) are greater than the cavity diameter of pillar[5]arene (d = 9.14Å), so the racemization of enantiomers is sufficient to be inhibited through Ar 3 B-substituents. The equimolar enantiomers of pS-P5BB and pR-P5BB are packed in a unit cell via C-H/O, C-H/p and C-H/C interactions (Fig. S8 †).
The photophysical properties of P5BB and PhBB were investigated in THF solution and the solid state ( Fig. 1 and Table 1). PhBB shows a strong absorption band of p-p* transition at 345 nm. However, P5BB exhibits two absorption peaks at 306 nm and 328 nm, corresponding to the local p-p* transitions in the pillar[5]arene motif and in the axial conjugated skeleton, respectively (Fig. S9 †). 22i In comparison to PhBB, P5BB showed signicantly red-shied emission in both the solid state and Scheme 1 Research foundation and design strategy of pillararenebased planar chiral charge transfer dye with amplified g lum values.
Scheme 2 (a) Synthetic approach of P5BB and PhBB. (b) X-ray crystal structure of P5BB in elemental colors (C: grey, O: red, B: green; ellipsoid probability = 50%). All the hydrogen atoms and solvent molecules are omitted for clarity. solution, which was ascribed to the ICT between the pillar[5] arene donor and Ar 3 B acceptor. The ICT nature could be veried by a visible solvatochromic emission in various polar solvents (Fig. S11 †). Owing to the temperature dependence of the equilibrium between the local excited (LE) state and the ICT excited state, 26 we explored the thermally responsive emission of P5BB in 2-methyltetrahydrofuran as a low melting point solvent (Fig. S12 †). At low temperature (150 K), P5BB showed a signicantly dual emission band with a main ICT emission at 552 nm (s = 33.9 ns) slightly overlapped with a minor LE emission at 405 nm (s = 2.3 ns). With temperature increasing from 150 to 330 K, the main emission band of P5BB experienced an apparent hypsochromic shi, and the emission color change from yellow to blue was monitored using CIE coordinates. There is a good linear relationship of the maximum emission wavelength versus temperature with a correlation coefficient of 0.976. It is noteworthy that the above thermochromic response is completely reversible. The good accuracy and reversibility suggested that the system is an ideal candidate for high-performance uorescent thermometers.
In order to further understand the correlation between the molecular structures and the photophysical properties of P5BB and PhBB, the electronic structure calculations were performed using DFT (B3LYP, 6-31G(d,p)) and TD-DFT (B3LYP, 6-31G(d)). TD-DFT computations revealed that the absorption of PhBB is mainly attributed to the p-p* transition to the S 1 state (HOMO / LUMO, f = 1.2994) ( Fig. S14 and Table S5 †). In P5BB, the HOMO is fully located on the electron-donor pillar[5]arene backbone; however, the LUMO is delocalized over the Bconjugated p-extension (Fig. S15 †). By means of TD-DFT calculations, it was found that the rst three CT transitions are the results of vertical excitations from the pillar[5]arenelocalized HOMO, HOMO−1 and HOMO−2, to the LUMO (Fig. S16 †). The higher excited states (S 4 and S 5 ) are excitations to the LUMO+1 level from the HOMO and HOMO−1. Cyclic voltammetry (CV) of P5BB showed a reversible reduction potential at −2.46 V (vs. Fc+/Fc, in THF), representing the reduction of the electron-decient Ar 3 B segments (Fig. S17 †). Three reversible oxidation curves with the rst oxidation potentials at +0.61 V for P5BB (vs. Fc+/Fc, in CH 2 Cl 2 ) were identied, corresponding to the oxidation of the pillar[5]arene skeleton. The electrochemical gap (3.07 eV) is almost consistent with the HOMO-LUMO gap (3.14 eV) via the DFT calculations. As expected, the rst oxidation potential of P5BB is slightly higher than that of compounds with the N donor (P5NN, The denite enantiomeric conguration inspired us to prepare their optically pure forms for studying the chiroptical properties. Two separated peaks with a 1 : 1 area were observed by the initial injection of rac-P5BB into a chiral HPLC with a Daicel Chiralpak IB N-5 column (hexane/2-propanol = 96/4, v/ v). Aer each fraction was well isolated with an enantiomeric excess (>99% ee, Fig. S18 †), the CD spectra of the enantiomers exhibited mirror-image relationships with strong CD absorption peaks in various organic solvents (Fig. 2a). The CD signals belonging to pillar[5]arene cores are clearly observed at 310 nm, 27 and the absorption dissymmetry factor jg abs j was calculated to be 1.53 × 10 −3 in hexane. On the basis of a comparison of the experimental CD absorption signals with the results of previous reports, 27 the pS congurations correspond to the rst peak and the second peak correspond to pR congurations in HPLC traces. In the CPL spectra, the enantiomers exhibited almost mirror-imaged signals in various solvents (Fig. 2b). The jg lum j reached 10 −3 in solution as well as in the solid state (Table 2), which is signicantly higher than that of P5NN and P5BN. The CPL spectra of P5BB gradually redshied with the increase in solvent polarity, and the positions of CPL signals were almost consistent with that of uorescence. In fact, the enantiomers of P5NN and P5BN did not show apparent CPL signals in various solvents (except for hexane), suggesting that the chiral transfer did not occur   effectively in this case. These phenomena indicated that the ICT character of P5BB not only amplies the g lum factors but also adjusts the color of CPL via the selection of different solvents. With all the necessary photophysical and chiral optical data in hand, the CPL brightness (B CPL : dened as B CPL = 3 × F × jg lum j/2) in solutions was further calculated to evaluate the overall performance of the CPL dyes. The B CPL of pS-P5BB was calculated to be 60.5 M −1 cm −1 in hexane, which is signicantly higher than that of pS-P5BN (15.9 M −1 cm −1 ), indicating that P5BB possesses excellent chiroptical properties for future CPL applications.
In order to further evaluate the chiroptical changes induced through host-guest chemistry, we selected the neutral small molecule 1,4-dibromobutane (G) as the representative guest to bind P5BB by 1 : 1 complexation. 28 As shown in Fig. S19, † upon the addition of excess 1,4-dibromobutane to P5BB in CDCl 3 solution, the proton signals of H 1-3 and H 5-11 on P5BB were shied downeld, and meanwhile, all signal peaks on the guest were found to shi upeld in 1 H NMR spectra. Additionally, the proton peaks of the guest were substantially reduced aer complexation due to inclusion-induced shielding effects. The association constant (K a ) was determined to be 187.8 M −1 using the nonlinear data tting of 1 H NMR titrations ( Fig. S20 and S21 †). This implies that host-guest recognition between P5BB and 1,4-dibromobutane has occurred. Notably, the addition of a guest results in dramatic enhancement of CD signals at 310 nm and 328 nm in CHCl 3 , while the absorption does not change signicantly, implying that the host-guest complexation decreases the conguration rotation of the pillar[5]arene skeleton of P5BB (Fig. 3). The addition of excessive 1,4-dibromobutane led to a decrease in uorescence intensity, and meanwhile, the CPL signals showed a mild enhancement, indicating the host-guest recognition further affected the excited state chiral conformation of P5BB.
Considering the Lewis acidity of Ar 3 B, another interesting thing is the response behavior of P5BB toward small Lewis bases. 29 Herein, the optical responses of four common halogen anions (including F − , Cl − , Br − and I − ) as Lewis bases to P5BB were primarily investigated in THF solution. In the UV-vis absorption spectra (Fig. 4a), the absorption bands at around 306 nm and 328 nm of P5BB gradually decrease with the addition of F − anions, which is due to the fact that the conjugation of the Ar 3 B moiety was broken by the formation of tetra-coordinated boron complexing with F − anions (Scheme S1 and Fig. S24 †). The limit of detection (LOD) was further calculated to be 43.3 nM based on the 3s/S values (Fig. S22 †). Remarkably, other halogen ions (Cl − , Br − and I − ) could not cause any signicant changes in absorption spectra, which was likely ascribed to the smaller steric size of the F − anion. Furthermore, the strong affinity between uoride and boron also played a crucial role. As displayed in Fig. 4b, as the concentration of F − increased, the emission peak at 510 nm of P5BB slowly declined, while the emission peak at 365 nm rapidly enhanced. Quite evidently, the emission spectrum exhibited a blue shi (∼145 nm) with the emission color changing from green to purple, and the uorescence intensity increased dramatically with F up to 56%. These phenomena indicated that the binding of F − anions with the Ar 3 B unit prevented the ICT process in the excited state, leading to the enhancement of LE-state emission. 29 Moreover, in the presence of an excess amount of F − anions, the CD signals of the enantiomers at 328 nm disappeared, while the signals at 310 nm were effectively enhanced. Even aer complexation with F − anions, signicant CPL signals with a blue-shi were also detected. The jg lum j values were calculated to reach 5.08 × Table 2 The chiroptical property data of P5NN, P5BN and P5BB  10 −3 with a B CPL of 47.6 in this case and were much higher than those of chiral pillararene derivatives reported in ref. 16b (Scheme S2 †). These investigations conrmed that the P5BB system could realize UV-vis absorption/uorescence/CD/CPL quadruple-mode sensing of F − anions.

Conclusions
In summary, we have developed an effective strategy to design and synthesize a p-conjugated planar chiral CT dye P5BB through integration of pillar[5]arene with organoborane. The ICT character in P5BB not only amplied the g lum values but also could tune the CPL color by the selection of various solvents with different polarities. Approximately 10-fold enhancements in g lum were observed from P5BB to P5BN in hexane, which was ascribed to the fact that the photo-responsive unit itself contains planar chiral pillar[5]arene in P5BB. Inclusion of the achiral guest 1,4-dibromobutane directly enhanced chiral optical signals, including CD and CPL. Furthermore, the coordination of F − anions with boron leads to remarkable changes in absorption, uorescence, CD and CPL signals of the P5BB system. Consequently, the P5BB platform could specically detect F − anions with high sensitivity and favorable selectivity. The current design strategy to fabricate a CPL-active ICT dye with the amplication of the g lum factor is expected to promote the development of future planar chiral pillararene materials. We envision that this work will catalyze the future application of pillararene-based CPL-active systems in chiroptical sensing, chiral supramolecular chemistry and CPL-based photoelectric devices.

Data availability
All the data supporting this article have been included in the main text and the ESI. †

Author contributions
J.-F. C. initiated and coordinated the study. J.-F. C., Q.-X. G. and T.-B W. designed all experiments, analyzed the data, and wrote the manuscript. L. L. carried out the computational work. P. C. and T.-B. W. supervised and administrated the project. All authors approved the nal version.

Conflicts of interest
There are no conicts to declare.