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A lateral ﬂ ow assay is combined with photothermal nanoparticles and thermochromic sheet for the detection of recombinant dengue virus serotype 2 NS1 protein. By optically stimulating nanoparticles, their temperature is raised and thus being sensed by thermochromic sheet, which results in coloured visual signal. This thermal sensing-based technique is cost-eff ective and shows signiﬁ cant sensitivity enhancement.


Introduction
Dengue fever is a mosquito-borne disease that can cause degrees of acute symptomatic illness from mild to life-threatening conditions in infected patients.There are four antigenically different serotypes of dengue virus (DENV) namely DENV1, DENV2, DENV3 and DENV4. 1 The most prevalent serotype contributing to dengue infection is DENV2. 2 The number of DENV infection cases has grown over the last two decades. 3,4There are approximately 390 million cases of DENV infection in the world every year and only 25% of them have apparent symptoms of dengue illness.The disease has been contagiously spread across tropical regions, particularly in Asia. 2,3,5Dengue infection has an adverse impact on global health and extra awareness is needed to control, provide surveillance and avoid the expansion of endemic DENV.
][9] Commonly, nucleic acids extracted from whole blood, serum, or plasma are amplified using a reverse transcription-polymerase chain reaction.On the other hand, a serological-based analysis seeks an antibody produced by the immune system in response to DENV.These analyses are highly specific and sensitive, yet they need to be handled by specialists, advanced laboratory equipment and expensive reagents.An alternative simple and rapid LFA test kit has been clinically utilized for detection of antigenic biomarkers, i.e. viral NS1 proteins. 9It was found that a paper-based assay is specific to the dengue virus, but the sensitivity needs to be improved to detect low levels of NS1 in the body.Hence, the outcome of LFA is usually confirmed by other standard methods to obtain a conclusive result.This limits the utility of LFA to be used in early diagnosis.
To address the existing issues with LFA, there have been several approaches to enhance its sensitivity, e.g.increasing the number of the binding sites on gold nanoparticles, that was designed to sit on a large silica substrate, 10 dual attachment of the labelling nanoparticles, 11 inducing a chemical catalytic reaction, [12][13][14] image processing 15 and magnetization measurement. 16Recent studies have exploited the use of photothermal nanoparticles in biosensing assays in which heat measurement can be achieved by monitoring either irradiation using an IR camera [17][18][19][20][21] or the conduction of heat. 17,22Other studies have reported the potential of using thermally sensitive material in biosensing applications based on the conversion of heat into a visual signal to enable a naked eye interpretation. 23,24o the best of our knowledge, liquid crystal thermochromic materials have never been integrated into an LFA system.We developed a colorimetric thermal sensing LFA to detect DENV2-NS1, the protein secreted from DENV2-infected cells.The visual signals received from the test line of a typical LFA and thermochromic signal intensity are compared in this study.Various photothermal nanoparticles, including MNPs and plasmonic nanoparticles, were investigated in this study to find the optimal nanoheater for the assay.They were illuminated with multi-wavelength light provided by an LED.The selected particles were used as visual signal providers for a typical LFA and as a light-to-heat converter for the thermal sensing assay.A liquid crystal thermochromic sheet served as a thermal sensor to display detectable coloration.The duration and power intensity of illumination were also optimized in this study.Quantification was determined by the use of customized MATLAB code. 25

Synthesis and characterization
2.2.1 Synthesis of MNPs.Polyol-capped IONPs and ZFNPs were produced based on the polyol-based method. 26The precursor for IONP synthesis was prepared by mixing vigorously 4 mmol of Fe(acac) 3 in 20 ml of TREG, followed by 2 h of sonication.The protocol has been slightly modified to yield zincdoped ferrite nanoparticles with polyol coating.To prepare zinc ferrite precursor, 174 mM of Fe(acac) 3 and 26 mM of Zn (acac) 2 were added to a tube containing 20 mL of TREG solution followed by vortex mixing and sonication for 2 h.
Both metal precursor solutions were transferred to 45 mL capacity Teflon liners, which were later loaded into alloy steel autoclave jackets.Their tops were closed by PTFE gaskets and sealed with screw caps before placing them in an oven (Memmert, model UFP400).The program was pre-set to immediately increase the temperature to 250 °C and maintained at this point for 8 h then ramping down to 20 °C and being held for 2 h.The excess solvent and by-products were removed by magnetic separation in acetone and DI water.The washing process was performed twice.Black pellets were retrieved and redispersed in DI water.
Then, trisodium citrate was added to the dispersion to reach 1000 mM and the mixture was gently shaken on a rotator mixer for 24 h at room temperature (RT).Unbound TREG and citrate in the aqueous solution were removed using a permanent magnet and rinsed several times with acetone and DI water.The final products were dispersed in water.
2.2.2 Synthesis of AuNSPs.Spherical gold nanoparticles were reproduced according to a previous study. 27Briefly, 50 mL of 0.03 wt% HAuCl 4 was boiled followed by the addition of 2.5 and 5 mL of trisodium citrate (1 wt%) with continuous stirring.The light-yellow solution turned to dark purple and eventually to ruby, indicating the success of the reaction.AuNSP solutions were left for 10 min at boiling point under stirring before the heat was removed.The solution was still continuously stirred at RT for another 20 min to ensure the completion of the reaction.
2.2.3 Synthesis of AuNRs.Rods with different aspect ratios were reproduced from the literature. 28,29The seed solution was formed by preparing a 10 mL mixture of 0.25 mM HAuCl 4 and 0.1 M CTAB.Subsequently, 0.6 mL of fresh ice-cold NaBH 4 (10 mM) was injected into the mixture.It was vigorously stirred and left undisturbed for 1 h to age the growth of gold seeds.
Second, 5 mL of 1.4 mM HAuCl 4 was mixed with 5 mL of 0.2 M CTAB to prepare the growth solution.Then, different volumes (75, 100 and 125 µL) of 10 mM AgNO 3 were added to the growth solution.This was followed by the addition of 1.25 M NaBr (0.25 mL) and 37% HCl (10 µL).Next, 79 mM of L-ascorbic acid (105 µL) was injected quickly and the mixture stirred for 30 s until it became colourless.During this step, the growth solution was formed.
Then, the freshly prepared growth solution was mixed with 60 µL of the seed solution.The mixture was incubated at 30 °C overnight to grow AuNRs.The particles were purified by centrifugation (8500 rpm) for 20 min.The supernatant was discarded, and the pellets were redispersed in water.Rod-shaped plasmonic nanoparticles were selected for use in the investigation due to their excellent photothermal properties.
2.2.4 Characterization.The morphology of the nanoparticles was revealed by transmission electron microscopy (TEM) at an accelerating voltage of 120 kV using a JEOL-1200 EX microscope with Gatan DigitalMicrograph software.The diameter and size distribution were determined using the free software ImageJ.Absorption spectra of the colloidal nanoparticles were obtained using ultraviolet-visible (UV-Vis) spectroscopy (SpectraMax M2, molecular device).

Thermal measurement
All colloidal particles were dispersed in water and adjusted to concentrations of 0.5, 1.0, 1.5 and 2.0 mg mL −1 .They were printed on the nitrocellulose membrane at 0.1 and 0.2 µL mm −1 using a bioreagent dispenser (Claremont BioSolutions LLC, Upland, CA).The pre-deposited nitrocellulose membranes were illuminated with a power intensity of 6.5 W cm −2 for 3 min.The temperature change was recorded using a thermal imager (Testo 875i) and was analyzed using "Testo IRsoft" software.The configurative setup for this thermal measurement is displayed in Fig. 1.A bare membrane was used as a negative control.Temperature measurement was per-formed in triplicate.The temperature gradient was determined as follows: where ΔT is the gradient of temperature, T average is the average temperature of the test line and T initial is the initial temperature of the membrane before illumination.

Aggregation assay and preparation of AuNSP@mAb
The conjugation protocol of antibody-conjugated AuNSPs (AuNSP@mAb) was optimized using a salt-induced aggregation assay, called a flocculation test.To find the optimum conditions for the antibody binding of AuNSPs, the pH of the assynthesised gold colloid with an optical density at 520 nm wavelength (OD 520 ) of 1.0 was altered using 0.1M K 2 CO 3 over a range between 6.5 and 9.5 with 0.5 unit intervals.200 µL of dispersion at each pH was transferred to a 96-well plate to which 10 µL of mAb was added to reach a final concentration of 20 µg mL −1 .The dispersion was vigorously mixed at RT for 30 min and 20 µL of 10% NaCl was then added to disturb the stability of the conjugates.After 10 min of incubation, the absorption was measured at wavelengths of 520 and 650 nm.
The ideal pH at which the dispersion was stable from the above experiment was chosen to determine the optimum antibody concentration.The mAb was added to interact with 200 µL of the as-synthesized AuNSPs (OD 520 = 1.0 and pH 9.0) in a 96-well plate with the final concentrations ranging from 5 to 20 µg mL −1 .The conjugates were kept under continuous rotation for 30 min at RT.This was followed by the addition of 20 µL of 10% NaCl and mixing for 10 min.The absorption spectrum of the conjugate was measured at 520 nm and 650 nm.
AuNSP@mAb for LFA operation was prepared based on the optimum conditions determined from the aggregation assay.The colloidal dispersion of AuNSPs (OD 520 = 1.0) was centrifuged and adjusted to pH 9.0 using 0.1 M K 2 CO 3 .Following this, the colloid was vigorously mixed with mAb at a final concentration of 10 µg mL −1 for 30 min.The conjugate was then blocked with 1% BSA for 30 min.Unbound mAb and BSA were removed by centrifugation at 14 000 rpm for 20 min at 4 °C.The washing step was repeated twice.The pellets were redispersed in the conjugate pad buffer (20 mM borate buffer, pH 9.0 supplemented with 1% BSA, 0.5% Tween 20, and 5% sucrose) and stored at 4 °C until use.

Fabrication of thermochromic LFA
The components of the colorimetric thermal sensing LFA are displayed in Fig. 2. It comprises similar parts to a general LFA with the integration of a liquid crystal thermochromic sheet.The nitrocellulose membrane (25 mm) was dispensed with 0.5 mg mL −1 of pAb (test line) and 0.3 mg mL −1 of goat antimouse IgG (control line) at 0.1 µL mm −2 using an automated lateral flow reagent dispenser (Claremont BioSolution LLC, Upland, CA).Antibodies were prepared in 10 mM PBS with 5% methanol to compromise the fixation of antibodies on the nitrocellulose membrane.A glass fiber conjugate pad (6 mm) was soaked with AuNSP@mAb solution for 30 min and left to dry overnight at RT.All components were assembled on an adhesive backing card with a 2 mm overlap between adjacent parts.A thermochromic sheet (5 mm) was attached under the area of the test line.The assembly was cut into 4 mm widths manually and stored in a desiccator at 4 °C until use.

Optimization of power intensity and length of illumination
The concentration of DENV2-NS1 was adjusted to 0.78, 1.5, 12.5 and 100 ng mL −1 in sample buffer (10 mM PBS, 0.5% BSA, and 0.1% Tween 20). 100 µL of the spiked sample was added to the thermochromic adhered strips.They were illuminated using an LED with power intensities of 5.2, 6.5 and 8.16 W cm −2 for 10 to 60 s, then the images of the thermochromic sheets were taken.The experiment was operated at 22 °C.

Assay procedures of typical and thermochromic LFA
DENV2-NS1 was prepared in a series of two-fold serial dilutions ranging from 0.78 to 400 ng mL −1 in a sample buffer (10 mM PBS, 0.5% BSA, and 0.1% Tween 20).Then, 100 µL of the diluent was transferred to the sample pad and the solution migrated through the strip by capillary force.The antigenic interaction between the AuNSP@mAb and DENV2-NS1 forms visible lines on the strip, whereas unreacted AuNSP@mAb and DENV2-NS1 were destined for the wicking pad.All experiments were performed in triplicate.Quantification of the typical visual signal and thermochromic signal is shown in Fig. 3. To obtain a typical visual signal contrast, images of the detection zone were acquired using a mobile phone to determine a quantification curve (Fig. 3A).After the completion of the conventional assay, the test line was illuminated using an LED light at 6.5 W cm −2 for 30 s.The photographs of the thermochromic sheet were immediately taken after turning off the LED using a mobile phone (Samsung Galaxy S9+) (Fig. 3B).Camera configurations (distance between object and camera, focal length, shutter speed, flash, and ISO: the level of sensitivity to illuminated light) were kept constant to eliminate external interference.The colour intensity of the signal was quantified by image processing using optimized MATLAB code with minimal effect of external light reflections. 25The analytical performance (sensitivity and the LOD) was assessed.The sensitivity is represented by the slope of the linear regression equation.To find the detection limit of the signal, the International Union of Pure and Applied Chemistry (IUPAC) method (eqn (2)) was used to determine the minimum signal that is distinguishable from the blank sample: [17][18][19]30 where S LOD is the LOD of the signal, S B is the mean of the signal received from the blank samples and σ B is the standard deviation of blank samples.

Characterization studies
The morphological information on the nanoparticles acquired by TEM as shown in Fig. 4. The MNPs are spherical 10.6 nm IONPs (Fig. 4A) and 12.0 nm ZFNPs (Fig. 4B).The diameters of AuNSPs are tunable depending on the volume of the added reductant.AuNSPs with a diameter of 12.0 nm were obtained with the addition of 5 mL of 1 wt% trisodium citrate (Fig. 4C), while 18.0 nm AuNSPs were produced by adding 2.5 mL of trisodium citrate (Fig. 4D).Histograms are shown alongside TEM images to represent the polydispersity of each nanomaterial.For the rod-shaped gold nanoparticles, by varying the volume of silver nitrate addition, the dimension of AuNRs can be tailored (Fig. 4E-G).The length of the rod was varied from 49 to 55 nm, whilst the width was between 31 and 25 nm.The aspect ratio of the rods is between 1.59 and 2.23.
Absorption spectra of all nanoparticle suspensions at 0.05 mg mL −1 in DI water were measured from 400 to 800 nm using a SpectraMax M2e.The MNPs have brown/black colour and absorb mostly in the shorter wavelength of the blue region (Fig. S1B †).The absorption intensity decreases at longer wavelengths where least absorption falls in the red region of the spectrum.In contrast, the plasmonic nanoparticles exhibited different optical patterns.Maximum light absorption was centered at 517 and 522 nm for the small and large AuNSPs, respectively.For AuNRs, two absorption peaks were observed, corresponding to the longitudinal and transverse peaks being the more intense and less intense peak, respectively.They exhibit different localized surface plasmon resonances (LSPRs) depending on their aspect ratios.AuNRs exhibit longitudinal LSPR peaks at 560, 600 and 625 nm for 75, 100 and 125 µL of silver nitrate (10 mM) added in the syntheses, respectively.The increase of silver nitrate concentration causes a red shift in the LSPR band and enhances the intensity, corresponding to the higher aspect ratio. 28,29

measurement
The thermal properties of the nanoparticles investigated using a broad-spectrum LED.To identify the most effective nanoparticles, their light-to-heat conversion capability on nitrocellulose membranes was investigated using an IR camera.In Fig. 5, ZFNPs were able to produce temperatures up to 25 °C at 2.0 mg mL −1 at a dispensing rate of 0.2 µL mm −1 whilst IONPs only raised the temperature up to 18 °C.This shows that at the highest dispensing rate, the nanoparticles exhibit the strongest photothermal activity.Moreover, it was observed that the most diluted concentration of IONPs and ZFNPs can raise the temperature distinctly above the temperature of the nanoparticle-free nitrocellulose membrane, emphasizing their great photothermal conversion performances toward white light.
Two different sizes of citrate-capped AuNSPs (12 and 18 nm diameters) were subjected to a photothermal experiment.Upon illumination, the temperature of the larger AuNSPs was not distinguishable from the null signal of the illuminated nitrocellulose membrane.The 12 nm AuNSPs reached a maximum temperature difference of 16.9 °C, being comparable to that of IONPs (17.0 °C), but less than that of ZFNPs (24.9 °C).The AuNRs with the LSPR at 560 nm were unable to raise the temperature above the threshold of background noise.To maximise the optical properties of the nanorods here, the aspect ratios were tuned to centralise the LSPR peaks at around 600 and 625 nm, which are around the region of the highest intensity provided by the LED (Fig. S1A †).The nanorods with the highest aspect ratio have stronger photothermal activity than those with the smaller ones, in terms of heat production, whilst being excited using a broad-spectrum LED.
Nanorods with medium aspect ratio having maximum absorption at 600 nm raised the temperature by 14 °C while nanorods with maximum absorption at 625 nm could increase the temperature by about 13 °C at 2 mg mL −1 (at the highest dispensing rate).The temperature of photo-excited nanoparticles is higher when they were deposited on the membrane at higher dispensing rate, due to the higher number of nanoparticles subjected to LED light.
ZFNPs show the highest temperature increase (24.9 °C) compared to other materials in this study.The resulting temperature is affected by the total amount of heat energy, the heat transfer process and the thermal conductivity of nanoparticles. 31Heat generation is larger when the collective effect of nanoparticles is taken into account, which is governed by the number density of the nanoparticles (eqn 3).When the mass concentration is 0.5 mg mL −1 , the particle number concentrations of ZFNPs and 12 nm AuNSPs are 1.04 × 10 14 particles per mL and 2.84 × 10 13 particles per mL, respectively.At a dispensing rate of 0.1 µL mm −1 and the same light intensity, 12 nm AuNSPs can raise the temperature to a similar level when compared to that of ZFNPs, even though they have 3.7 times fewer nanoparticles.Therefore, in this study, the 12 nm AuNSPs were chosen as the heat provider for the initial development of thermochromic LFA.

Aggregation assay
3][34] As the surface charge depends on the pH of the solution, it is important to find the appropriate pH value that can stabilize AuNSP@mAb in the presence of electrolytes.It can be seen that a decrease in the pH value tends to develop an aggregation of the conjugate (Fig. 6A).In the test, an excess amount of mAb was added to the solution to ensure sufficient antibody coverage.A basic pH of 9.0 is found to be the favoured point for conjugation to take place as it provides the most stability.The flocculation test continued with a chosen pH of 9.0 to identify the minimum amount of mAb to build a protective layer preventing aggregation (Fig. 6B).In the absence of mAb, the AuNSPs could not tolerate salt destabilization.The conjugate begins to plateau at a final concentration of 10 µg mL −1 of mAb.This concen-  tration is the minimum at which it can stabilize the conjugate by steric repulsion.should be noted that antibody coupling by force is weak and they are prone to detach in certain environments.This could lead to an aggregation of the AuNSP@mAb, as a result, the capture efficiency decreases because the binding sites are unavailable. 35Overcoating the antibody is also found to be unfavourable because of high non-specific binding. 19This emphasizes the necessity to determine the optimal dose of antibodies used as it affects the antigen-capturing activity.However, since different types of antibodies have different morphological structures, an empirical test is therefore required to find the critical conditions for the conjugate to maximize the performance of AuNSP@mAb in each study.It is shown that the antibody attachment in this study reaches an optimum in a solution at pH 9.0 and the concentration of added mAb at 10 µg mL −1 .

Optimization of power intensity and length of illumination
Images of the activated thermochromic sheet are shown in Fig. 7.The strongest power intensity of 8.16 W cm −2 was not used for further experiments because the thermochromic sheet is triggered even with a DENV2-NS1-free sample after 10 s of illumination.On the other hand, 5.2 W cm −2 is not able to generate a thermochromic signal at low concentrations of DENV2-NS1.The LOD is found at 12.5 ng mL −1 when the illumination was performed for 20 s.For the medium power intensity of an LED at 6.5 W cm −2 , it allows the detection of DENV2-NS1 at 1.56 ng mL −1 as the colour spot was observed on the thermochromic sheet.Therefore, the optimum conditions of illumination were found to be a power intensity of 6.5 W cm −2 and 30 s of illumination.

Sensitivity comparison
Further experiments investigate the sensitivity of DENV2-NS1 detection based on the observation of normal visual and ther-mochromic signals.The colourations of typical and thermochromic LFA are shown in Fig. 8.For the typical LFA, after the completion of sample migration, only a control line is visible in the absence or short of DENV2-NS1 in the sample, otherwise, the test is invalid.On the other hand, double lines appear on the nitrocellulose membrane indicating a positive result.The test line is observed by the naked eye with confidence at 6.25 ng mL −1 , which is the LOD of the typical assay (Fig. 8A).
The low number of analytes in the sample is not able to generate strongly visible test lines.Therefore, instead of directly reading the result, the strip was optically induced to produce heat energy to activate the thermochromic sheet and create a distinct signal.According to Fig. 8B, the method develops an evident red-pink signal at 1.56 ng mL −1 .The visual readout of the thermochromic signal reduces the LOD of the LFA by 4 times compared to the conventional LFA.Moreover, it is interesting to note that the colour transition is dependent on the concentration of DENV2-NS1.This finding is in agreement with a previous study that demonstrated the potential to identify the correlation between the thermally induced visual signal and the number of cancer biomarkers. 23In this study, the minimum temperature activation point is triggered.Subsequently, green and blue pixels grow and spread over the sheet when the concentration of DENV2-NS1 increases.The area of the thermochromic signal expands over the illumination time due to heat map expansion.
Herein, the intensity of the signal was quantified with the assistance of image processing technology.The colour intensity in each pixel of the test line and the thermochromic signal is digitally derived into red, green and blue (RGB) coordinates using a Bayer filter.The contrast between the positive signal and the background was calculated to establish a contrast index for comparison purposes.The colour intensity ratio is an indicator that refers to the test line appearance compared to the background.
The relationship between the concentration of DENV2-NS1 and the intensity ratio is plotted on a log scale as shown in Fig. 9.For the typical colorimetric readout (Fig. 9A-C), the intensity ratio of each colour channel exhibits a similar linear  regression trendline in the range of 0.78 400 ng mL −1 (R 2 = 0.99258, 0.99588 and 0.99566 for red, green and channels, respectively).The green channel is the most sensitive and exhibits the largest intensity signal ratio.A similar finding in the literature when a Bayer filter is used for quantitative analysis of the LFA in which it was suggested that the difference in light scattered by the accumulation of AuNSPs at the test line and the background is most sensitive to the region of the green channel in the Bayer filter. 15Unlike other thermal sensing LFA formats, the one developed in this study converts heat into a thermochromic signal allowing the quantification of colour intensity.The colour transition of the thermochromic sheet is dependent on the activating temperature resulting in a mixture of primary colour components.The RGB values were obtained from the images used for intensity ratio calculations.In Fig. 9D, the signal intensity ratio of the red channel increases from 1.56 ng mL −1 as the red pigment is developed and is locally dominant on the thermochromic sheet.The turning point is found at 25 ng mL −1 , as it creates a very large variation before a sharp decrease (R 2 = 0.92498).the other hand, when the analyte is over 12.5 ng mL −1 , the thermochromic sheet develops green and blue pigments while diminishing the red colour, causing a shift in the luminescent region of the Bayer filter.A similar linear trend is observed from 0.78 to 400 ng mL −1 for the intensity of the green (Fig. 9E) and blue (Fig. 9F) channels.The R 2 coefficients of the green and blue channels are 0.98066 and 0.93857, respectively.Considering the linear working range, the green and blue channels are more effective for analysis than the red channel.However, the intensity ratio of the green channel offers a slightly larger signal than that of the blue intensity ratio.
The sensitivity and LOD between each Bayer's channel are listed in Table S1.† The acceptable positive signal is the critical value exceeding by at least 3 times the signal of the null con-centration sample.Any signals above the level that can be distinguished from the background are treated as positive results.The typical LFA offers the detection limit of DENV2-NS1 at 6.25 ng mL −1 for the readout by the naked eye and image analysis.The thermochromic LFA outperforms the typical LFA as it offers greater sensitivity for every colour channel.The LOD of the red channel is 2 times lower than that of the typical LFA but the working range is narrower, hence, the red channel of the thermochromic LFA is ignored.The green and blue channels provide 4-time lower LOD than that of the common LFA.Therefore, considering that the green channel is more sensitive, it is the optimum channel for quantitative analysis.
To facilitate users in terms of readability of the assay, instead of quantification via MATLAB software, estimation of the DENV2-NS1 concentration can be conducted by the observation of the colour transition of the thermochromic sheet.In Fig. 10, when the concentration of DENV2-NS1 is below 1.56 ng mL −1 , colour transition is not observed.When the concentration of DENV2-NS1 ranges between 1.56 and 12.5 ng mL −1 , the red colour appears on the thermochromic sheet.The pres-  ence of green colour on the thermochromic sheet is detected when the is 12.5 ng mL −1 .This allows users to quickly estimate the range of concentration without the requirement of an IR camera, a digital temperature sensor, and image processing.

Discussion
It has been reported that the level of circulating DENV-NS1 in infectious patient serum samples is in the range between 3.64 and 265.7 ng mL −1 6 or 7 and 284 ng mL −1 . 36In the literature, dengue virus can be detected at 4 ng mL −1 (ref.8) and 4.9 ng mL −1 (ref.37) by immunoassay.Tran et al. developed a highly sensitive LFA using an enzyme to amplify the signal with the capability of detecting DENV2-NS1 at 0.1 ng mL −1 . 38The median circulating level of DENV2-NS1 was 8.26 ng mL −1 from serum samples collected in the first two days after onset of the fever. 6In children, it is reported that the level of free DENV2-NS1 is approximately 2-2.6 ng mL −1 on day 2 of the illness. 39he developed thermochromic LFA in this study shows a detection limit at 1.56 ng mL −1 with a dynamic range from 0.78 to 400 ng mL −1 , which covers the range of circulating DENV2-NS1 concentrations found in patients.These data emphasize the potential of using the thermochromic technique in early dengue diagnosis.However, determining the progression of dengue disease depends on the condition of patients, such as primary and secondary infection. 9he heat production of nanoparticles (Q, W cm −3 ) is dependent on the concentration of nanoparticles (N, the number of particles per mL), the absorption cross-sectional area (C, m 2 ) and the light intensity (I, W m −2 ): 17 It is seen that the number of AuNSPs is directly proportional to the extent of heat creation.The higher the concentration of DENV2-NS1, the higher the number of illuminated nanoparticles and the temperature built up.
The orientation of plasmonic particles has great impact on the ability to produce heat.The shorter the distance between each nanoparticle pair, the higher the temperature produced, and vice versa. 31When the concentration of DENV2-NS1 is low, the degree of thermochromic signals from the green and blue channels rises slightly followed by a sharp increase at 12.5 ng mL −1 (Fig. 9E and F).This is likely associated with an uneven distribution of AuNSPs throughout the area of the test line, due to the limited number of particles interacting with the test line antibody at low DENV2-NS1 concentration.On the other hand, when the AuNSPs are densely packed, they can produce a higher temperature.
Moreover, the morphology of nanoparticles plays an important role in the photothermal effect because of their different optical properties. 40,41There are two shapes of gold nanoparticles that were investigated for their heat production, including nanospheres and nanorods, where the absorption spectrum is displayed in Fig. S1B.† The 12 nm nanosphere exhibits the greatest response to the used LED, so it is selected as a light-to-heat converter for the thermal sensing LFA in this study.In theory, the cross-sectional absorption area is directly proportional to photo-thermalization.However, it is applicable for the prediction of a single nanoparticle, but not for nanoparticle clusters in this case. 42At the same total volume of nanoparticles, the ensemble of small nanoparticles yields a higher temperature than that of the larger one, due to the collective effect. 31,42Moreover, in terms of heat dissipation, the smaller nanoparticles have less heat capacity than the larger ones, hence they can release excess radiative energy faster and more effectively. 43Another possible explanation is the enhancement of the scattering effect as the dimension of the nanoparticles increases. 42,43Re-absorption and re-scattering of light have a vital role in heat production.However, since the nitrocellulose membrane scatters only a partial amount of incident light, and the particles are surrounded by a medium of air, the probability of re-absorption occurring is reduced, and thus a decrease in plasmonic heating was observed.
One of the critical factors to be considered when using the colorimetric thermal sensing LFA, is the domination of ambient temperature.The thermochromic sheet has various working temperature bandwidths as specified by the manufacturer.A necessary step is added to tune the power intensity of light and the illumination time to fit with the ambient temperature to avoid misinterpreting the result.
According to eqn 3, if the concentration and the diameter of AuNSPs are kept constant, the heat production is proportional to the power intensity of illumination.The length of exposure time also needs to be controlled carefully during the operation.The temperature induced by illumination has a tendency to rise over time followed by a steady state at around the maximum temperature.Moreover, the heat can be accumulated and spread over the thermochromic sheet depending on the length of exposure.Prolonging the illumination period can increase the signal, but at the same time, it can cause a false positive result.Therefore, appropriate illumination time and power intensity need to be specifically determined for each application in order not to oversupply photons to the thermal sensing system, otherwise, the thermochromic signal is detectable even in the absence of biological targets.
Thermal sensing measurements usually require background subtraction to eliminate the noise signal.Although the nitrocellulose membrane has poor photothermal effect, light can be absorbed by biological molecules in the membrane resulting in high noise background. 19Blocking agents are essential to minimize the non-specific binding effect of the nitrocellulose membrane and AuNSP@mAb, nonetheless, they cannot completely remove the undesired noise signal from the system.The use of strong photothermal nanoparticles can potentially solve the problem by creating a massive thermal signal to ensure that the thermochromic signal is far above the noise background signal so that it can be discriminated. 21The thermochromic sheet can absorb a certain amount of light, leading to a rise of unnecessary temperature attributed to noise.Future work should focus on the use of a filter or a single wavelength light source, or alternative thermally sensitive materials, to eliminate undesirable signals.

Conclusion
We have developed a thermochromic paper-based DENV2-NS1 detection.Different types photothermal nanoparticles were produced for thermal measurements and 12 AuNSPs were selected for the development of a thermochromic LFA.Lateral flow strips were quantified by image processing.The thermochromic LFA has a similar linear detection range (0.78-400 ng mL −1 ) to that of the typical LFA.We found that the thermochromic LFA is significantly more sensitive than the conventional LFA.The test line of the conventional assay is observable at 6.25 ng mL −1 whilst the thermochromic signal is visually detectable at 1.56 ng mL −1 .
We also demonstrate the possibility of this thermochromic technique for semi-quantitative analysis by simply observing the colour of the thermochromic sheet using a mobile phone camera.This feature does not need image processing analysis and gives a quick result to users.The colour alteration of the thermochromic sheet is observed in the sequence of colours, which correlates to the level of DENV2-NS1 analytes (black = <1.56ng mL −1 , red = 1.56-12.5 ng mL −1 , greenish blue = >12.5 ng mL −1 ).
In conclusion, our thermochromic LFA is a fast, simple, and low-cost technique that offers great sensitivity of the detection of DENV2-NS1 with a wide concentration working range.This can be a promising technique for expanding the utilities of LFA to be used in early clinical diagnosis.

Fig. 1 A
Fig. 1 A diagram of thermal measurement.Nanoparticles on the nitrocellulose membrane are illuminated with an LED.Thermal recording is achieved using an IR camera.

Fig. 3
Fig. 3 Schemes represent the image acquisition and calculation of the visual intensity ratio signal for (A) a typical visual assay and (B) a colorimetric thermal sensing assay.The colour intensity is quantified by image processing.The contrast of the typical visual readout can be obtained by dividing the colour intensity of the background by that of the test line.Inversely, the contrast of thermal visual signals is calculated by determining the ratio of the thermochromic signal to the colour intensity of the thermochromic sheet.

Fig. 2
Fig.2The architecture of the colorimetric thermal sensing LFA.It is composed of a sample pad, a conjugate pad, a nitrocellulose membrane, test and control lines, a wicking pad, and a thermochromic sheet.

Fig. 4
Fig. 4 TEM images and size distributions of MNPs and plasmonic nanoparticles.The top row represents the morphological information of (A) IONPs, (B) ZFNPs, (C) AuNSPs to which is added 5 mL of trisodium citrate and (D) AuNSPs to which is added 2.5 mL of trisodium citrate.The bottom panel displays the morphology and aspect ratio of AuNRs to which different volume of 10 mM AgNO 3 is added (E) 75 µL, (F) 100 µL and (G) 125 µL.

Fig. 6
Fig. 6 Aggregation tests for AuNSP@mAb conjugation.(A) The effect of pH and (B) the effect of antibody concentration.

Fig. 5 (
Fig. 5 (A) and (B) Relationships between the temperature gradients and mass concentrations of IONPs, ZFNPs, 12 nm AuNSPs and 18 nm AuNSPs at different dispensing rates and (C) and (D) those of AuNRs with different aspect ratios.

Fig. 8 (
Fig. 8 (A) The strip for typical LFAs and (B) thermochromic sheets of the colorimetric thermal sensing LFA.They were tested against DENV2-NS1 at various concentrations ranging from 0 to 400 ng mL −1 .

Fig. 9
Fig. 9 Logarithm graphs present the relationship between the visual intensity ratios of three primary colours (RGB) and the DENV2-NS1 concentration (0-400 ng mL −1 ) of typical and thermochromic LFAs: (A-C) typical LFA and (D-F) colorimetric thermal sensing.The * indicates the LOD of that colour channel.Dashed lines are linear trend lines (n = 3).

Fig. 10
Fig.10Semi-quantification method.The concentration range of DENV2-NS1 is represented by the appearance of the colour of the thermochromic sheet.