Real-Time Visualization of Latent Fingermarks with Level 3 details based on Solid State Emissive Organic Fluorophore Using Powder Dusting Method

Herein, we have designed and synthesized a water-soluble organic fluorescent probe (Py-OH, 3) and applied it for latent fingermarks (LFMs) detection via powder dusting method. The probe is highly emissive...


Single Crystal X-Ray Diffraction:
The single crystal X-ray diffraction data of the Py-Pr-OH crystals were collected on a Microfocus D8 venture Bruker APEX 3 diffractometer equipped with a CCD area detector and having MoKα radiation (λ = 0.71069Å).SAINT program (v. 8.38A) was used for data reduction, which were analyzed for agreement using XPREP.All these software were included in the APEX 3 software suite (v 2017.3-0 ).S1 Absorption correction was carried out with the SADABS program.S2 The crystal structure were solved using SHELXT (version 2018/2) S3 program and refined by refined using SHELXL-2014.S4-S6 .The positions of H-atoms were calculated and refined isotopically.Crystallographic data of Py-Pr-OH have been deposited with the Cambridge Crystallographic Data Centre (CCDC) under deposition no 2249837 and the crystallographic parameters are given in Table S1, supporting information.

LFM development Powder preparation procedure
To the suspension of Py-Pr-OH in DCM, silica gel (230-400 mesh) was added.The solvent was evaporated in rotary evaporator.The silica adsorbed material was further grinded to make it free flow, and was further used for LFMs development procedure.

LFM development procedure by powder dusting method
FMs were collected from volunteers, asked to touch their fingers lightly on the chosen substrate surfaces such as tinfoil, paper currency, ceramic tile, plastic, plywood, CD, coin etc.).The LFM developing fluorescent smoothly brushed on their surfaces the images were captured using mobile phone camera.
Figure S3.a) Packing of the molecules when viewed along c-axis.b) Packing of the molecules when viewed along b-axis S5 6.

RFigure S3 .
Figure S3.a) Packing of the molecules when viewed along c-axis.b) Packing of the molecules when viewed along b-axis.

Figure S4 .
Figure S4.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in methanol.

Figure S5 .
Figure S5.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in ethanol.

Figure S7 .
Figure S7.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in THF.

Figure S8 .
Figure S8.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in acetonitrile.

Figure S9 .
Figure S9.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in DMF.

Figure S10 .
Figure S10.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in DMSO.

Figure S11 .
Figure S11.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in water.Quantum yield calculation

Figure S13 .
Figure S13.LFM developed on various non-porous substrates such as currency, ceramic tile, plastic, plywood, CD, coin A) under daylight, B) under UV light.

Figure S15 .
Figure S15.LFM developed on aluminium foil for aged sample a) after 7 days a') after 15 days in ambient atmosphere and Stained sample b) before treatment; b') After treatment with Py-Pr-OH.

Figure S16 .
Figure S16.LFM developed on surfaces of common objects like a) coffee mug b) leather wallet c) calculator d) cardboard e) stapler under under day light a') coffee mug b') leather wallet c') calculator d') cardboard e') stapler under 365 nm UV light.

Table S2
. Solvent dependent properties of Py-Pr-OH in different solvents S5 7. Figure S4.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in methanol.S7 11. Figure S8.Fluorescence spectra of Py-Pr-OH (10 µM) with different excitation wavelengths at room temperature in ACN.

Table S1 .
Single crystal X-ray parameters of Py-Pr-OH at 298 K

Table S2 .
Solvent dependent properties of Py