Photo-switchable anion binding and catalysis with a visible light responsive halogen bonding receptor †

Photo-switchable receptors allow for photo-control over guest binding and release with spatial and temporal precision. Here we report the first halogen bonding photo-switchable anion receptors in which chloride binding may be reversibly modulated by irradiation with red and blue light, with over a 50-fold enhancement in chloride binding aﬃnity observed for the Z isomer. We demonstrate that this switchable binding enables unprecedented photo-controlled catalysis of XB-mediated halide abstractions and a Mukaiyama Aldol reaction.

The recognition of anions by synthetic supramolecular receptors is a rapidly expanding field of research, driven by applications in sensing, catalysis and transmembrane transport. 1,2mongst the array of Lewis acidic intermolecular interactions available for anion recognition, halogen bonding has emerged in recent years as a highly effective interaction for strong and selective anion binding. 35][16][17] The stringent linear interaction and resistance to solvent effects 18 are particularly beneficial for the design of potent anion receptors.
Photo-switchable receptors, in which guest binding is modulated by photo-isomerization of the host in order to switch between strongly and weakly binding states, enable control over when and where guests are bound and released.Since Shinkai's work on potassium hosts, the field of photo-responsive cation receptors has become relatively well established. 19However, photoresponsive receptors for anions remain rare, 20 with only a handful of systems reported to date based on azobenzene foldamers 21,22 and receptors, [23][24][25][26][27] azopyrazoles, 28 acylhydrazones 29 or stilbene photoswitches, [30][31][32][33] all exploiting hydrogen bonding interactions for anion recognition, and apart from two exceptions reported by us, 24,25 all responding to UV light, which has poor biocompatibility and limits applications in biological anion sensing or transport.
Here we report the first example of a halogen bonding, visible light responsive anion receptor, by functionalizing a red-shifted ortho-chloro azobenzene with electron deficient iodo-triazole motifs.The receptor can be switched from a weakly binding state as the E isomer to a strongly binding state as the Z isomer using red light, and this process may be reversed using blue light.We also demonstrate that the charged iodo-triazolium analogue acts as an unprecedented XB-mediated photo-switchable catalyst, in which the Z isomer is a more potent catalyst for a variety of model XB-catalyzed reactions.
The target halogen bonding photo-switchable receptor is shown in Fig. 1, which incorporates an ortho-chlorofunctionalized azobenzene.5][36][37][38][39] Chloro-substitution enables red light switching, and affords long Z isomer lifetimes (half-life of B1 week), 25 which is particularly advantageous for switchable binding applications, where thermal relaxation would lead to uncontrollable ion release.The central azobenzene core is functionalised with electron deficient aryl-iodotriazoles, which are potent XB donors. 12,40,41eceptor 1 was prepared from commercially available carboxylic acid 2, which was reduced and subsequently protected to afford aniline 4. Azobenzene 5 was generated in good yield with in situ generated DBU-Cl + in the presence of 4 (Scheme 1). 42fter deprotection, alcohol 6 was tosylated and subsequently converted to the azide 8, which was subjected to Cu-catalyzed 'click' conditions with iodoalkyne 9 to afford receptor 1 in good yields.
We first investigated the photo-switching properties of 1. Irradiation with red light from an LED (625 nm, B0.9 W) afforded high conversion to the Z isomer with a photostationary state (PSS) distribution of 83% Z in acetone as determined by 1 H NMR experiments (Fig. S49, ESI †).Irradiation with blue light (455 nm, B1.1 W) triggered Z to E isomerisation (87% E).It was also possible to generate nearquantitative amounts of 1 E by heating to 60 1C in the oven for 1 h (4 98% E).The UV-vis spectra of 1 are shown in Fig. 2A.As observed in related tetra ortho-chloro azobenzenes, the n-p* transitions are well separated, as reflected in the high PSS in both switching directions.The switching process is fully reversible and could be repeated multiple times without detectable fatigue (Fig. 2B).
Molecular mechanics calculations of 1 Z as a complex with chloride suggested that both iodotriazole XB donors are capable of forming a convergent bidentate interaction with the anion (Fig. 2C).We therefore investigated the anion binding capability of the receptor by conducting 1 H NMR titration experiments in d 6 -acetone by addition of aliquots of the anion as the tetrabutylammonium salt.The data were fitted to the appropriate binding isotherms using Dynafit, 43 and the association constants are shown in Table 1.K 1 , E is the 1 : 1 binding constant for anion binding by 1 E and K 1 , Z is the corresponding 1 : 1 binding constant for 1 Z , which was determined by generating the Z-rich PSS in the NMR tube with red light (83% Z), and accounting for binding to the minor population of 1 E in the analysis.
Chloride binding to 1 E was statistical (binding to one iodotriazole XB donor is independent of that to the other). 33or statistical binding, K 1 = 4K 2 by accounting for the statistical factors, and K 1 is 2K ref , where K ref is the binding affinity to an individual iodo-triazole binding site.We have previously determined this single site binding affinity to be 197 AE 14 M À1 in a related 3,5-bistrifluoromethyl aryl-iodotriazole, in which the pendent azobenzene in 1 is replaced by a hexyl group. 15In this case the binding affinity to one site of 1 E is 296 M À1 .This suggests that the adjacent azobenzene leads to a modest enhancement of chloride affinity, further evidenced by chemical shift perturbations of the adjacent H atoms on the azobenzene aryl rings (Fig. S59-S64, ESI †).Binding of the halides to 1 E decreased in the order Cl À 4 Br À 4 I À ; and no binding of either nitrate or hydrogen sulfate was observed.
The affinity of the corresponding Z isomer 1 Z to the halides was much enhanced compared to the E isomer.In the case of chloride this enhancement was significant, with K Z /K E = 53, the highest reported to date for a switchable anion receptor incorporating halogen bonding interactions, 33 and amongst the highest known across all anion receptor types. 20This provides strong evidence that both iodotriazole XB donors are involved in a bidentate chelating cooperative interaction with the anion.
We quantified the chelate cooperativity in the system through the effective molarity, EM, which defines the concentration at which simple intermolecular interactions compete with cooperative intramolecular interaction (Table 1 and eqn ( 1)). 44

EM = K
The extent to which the XB anion complex, with cooperative intramolecular halogen bonds from both XB donors, is populated is given by K ref EM.This is notably large in the case of chloride binding (K ref EM = 107), indicative of a highly cooperative anion binding process.The anion binding enhancement for 1 Z over that of 1 E is reduced for Br À and I À , suggesting that the binding cavity formed by the two convergent XB donors in the Z isomer is optimized for the smaller chloride anion.
Strong cooperative binding of 1 Z to Cl À results in a 3-fold enhancement in thermal half-life relative to the free receptor (Fig. S35-S41, ESI †).In contrast, in the presence of a noncoordinating anion (PF 6 À ) no change in half-life is observed.This represents a rare example of modulating thermal half-life stability via an anion binding event.
We also prepared the iodotriazolium receptor 1-Me-Bar F by methylation and subsequent anion exchange of 1 (Fig. 3A, see ESI † for synthesis and characterization).As expected, the affinity of the dicationic host for chloride was enhanced relative to 1, necessitating the use of the more competitive solvent DMSO-d 6 for titration experiments for the triflate derivative.The switchable anion affinity was maintained in this system, albeit significantly diminished (K 1 . This may be due to charge repulsion in the Z-isomer where both binding sites are in close proximity. The ability of the photo-switchable halogen bonding anion receptors to catalyze a Friedel-Crafts alkylation by bromide abstraction was then explored (Fig. 3B). 45Both isomers of 1-Me-BAr F were able to efficiently catalyze this benchmark reaction at low concentrations (1.7 mM), with notably a B3-fold rate enhancement for 1 Z -Me-BAr F both isomers of the neutral analogue 1 were unable to catalyse the desired reaction (Fig. S72-S73, ESI †), suggesting that charge-assisted halogen bonding is essential for this reaction to take place; in line with Huber's work. 45We observed significantly diminished rates for the reaction in the presence of 2 equivalents of control compound 10, and no background activity in the absence of an activator (Fig S71 -S80, ESI †).
Encouraged by this result, we screened a variety of other benchmark XB-mediated reactions.Pleasingly, our system efficiently catalyzed the analogous chloride abstraction reaction (Fig. 3C and Fig. S81-S88, ESI †), with a B2-fold enhancement in rate for the Z-isomer relative to the E-isomer.1-Me-BAr F 4 was also capable of catalyzing a Mukaiyama aldol reaction at 0.5 mol% catalyst loadings (Fig. 3D and Fig. S89-S96, ESI †), 46 with the Z-isomer affording approximately twice as much product relative to the E-isomer within 7 hours.Finally, 1-Me-BAr F    although there was no significant discrimination between the isomers.This is perhaps due to the steric demands of the enone within the relatively small binding site of 1 Z -Me-BAr F 4 , leading to monodentate binding modes.In all benchmark reactions, two equivalents of control compound 10 relative to 1-Me-BAr F resulted in sluggish reactions, suggesting that the dicationic nature of 1-Me-BAr F 4 enhances catalysis rates.In conclusion, we report the first visible light switchable anion receptor and catalyst utilising halogen bonding interactions.The neutral bis-iodo-triazole functionalised red-shifted azobenzene can be switched between a weakly binding E isomer and the strongly binding Z isomer using blue and red light, with over a 50-fold enhancement in binding affinity observed.The analogous dicationic triazolium system enables unprecedented photo-regulated XB-mediated halide abstraction catalysis of a Friedel-Crafts alkylation and Mukaiyama aldol reaction, in which the stronger binding Z isomers acts as the more potent catalytic species.These results demonstrate that combining molecular photoswitches with potent halogen bond donors could open up new applications of photo-responsive receptors for achieving spatio-temporal control over both molecular recognition and catalytic processes using light.
A. K. acknowledges the EPSRC Centre for Doctoral Training in Synthesis for Biology and Medicine for a studentship (EP/L015838/1), generously supported by AstraZeneca, Diamond Light Source, Defence Science and Technology Laboratory, Evotec, GlaxoSmithKline, Janssen, Novartis, Pfizer, Syngenta, Takeda, UCB and Vertex.M. J. L. acknowledges funding from the Royal Society.M. J. L. is a Royal Society University Research Fellow.

Fig. 2 (
Fig. 2 (A) Absorption spectra of 1 as the E and Z isomer.Inset: np* bands and composition of the PSS mixtures upon irradiation with red light (625 nm, determined by NMR integration).Pure Z-isomer spectrum was calculated from the spectra of pure 1 E and the PSS mixture of known composition.(B) Absorption at 445 nm after successive cycles of irradiation with 625/455 nm light (acetone).(C) Geometry optimised molecular mechanics calculated structure of 1 Z Cl.

Fig. 3 (
Fig. 3 (A) Structure of iodotriazolium receptor and control compound.(B and C) Friedel-Crafts alkylation reaction performed with activator 1-Me-BAr F 4 .Data shown in presence and absence of activator for each isomer (black points) and fit to first order rate equation (lines).(D) Mukaiyama aldol reaction with activator 1-Me-BAr F 4 .

Table 1
Anion binding data a Anions added as the tetra butyl ammonium salt in d 6 -acetone, 298K.b No binding.Errors at the 95% confidence limit.