Issue 5, 2024

A lysosome-targeted fluorescent probe based on a BODIPY structure for Cys/Hcy detection

Abstract

Cysteine (Cys) and homocysteine (Hcy) are important biothiols in living organisms. They play important roles in a variety of physiological and pathological processes. Therefore, it is very important to design an optical probe for the selective detection of Cys/Hcy. Herein, we report the design and synthesis of a fluorescent probe NBD-B-T based on a boron-dipyrromethene (BODIPY) structure, which showed an excellent lysosome targeting ability and an outstanding Cys/Hcy detection capacity. For NBD-B-T, the sensing group 7-nitro-2,1,3-benzoxadiazole (NBD) and the lysosomal targeting group morpholine were introduced. The results show that the NBD-B-T probe can detect Cys/Hcy with fluorescence emission turn-on performance. The low detection limits of this probe are about 76.0 nM for Hcy and 97.6 nM for Cys, respectively. The NBD-B-T probe has a low detection limit, high stability, and excellent selectivity and sensitivity. More importantly, the NBD-B-T can target lysosome, and simultaneously detect the Cys/Hcy in living cells.

Graphical abstract: A lysosome-targeted fluorescent probe based on a BODIPY structure for Cys/Hcy detection

Supplementary files

Article information

Article type
Paper
Submitted
07 Nov 2023
Accepted
27 Dec 2023
First published
27 Dec 2023

Anal. Methods, 2024,16, 686-694

A lysosome-targeted fluorescent probe based on a BODIPY structure for Cys/Hcy detection

W. Zhang, B. Wu, M. Liang, M. Zhang, Y. Hu, Z. Huang, X. Ye, B. Du, Y. Quan and Y. Jiang, Anal. Methods, 2024, 16, 686 DOI: 10.1039/D3AY01965A

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