Enzyme assays with supramolecular chemosensors – the label-free approach

Enzyme activity measurements are essential for many research areas, e.g., for the identification of inhibitors in drug discovery, in bioengineering of enzyme mutants for biotechnological applications, or in bioanalytical chemistry as parts of biosensors. In particular in high-throughput screening (HTS), sensitive optical detection is most preferred and numerous absorption and fluorescence spectroscopy-based enzyme assays have been developed, which most frequently require time-consuming fluorescent labelling that may interfere with biological recognition. The use of supramolecular chemosensors, which can specifically signal analytes with fluorescence-based read-out methods, affords an attractive and label-free alternative to more established enzyme assays. We provide herein a comprehensive review that summarizes the current state-of-the-art of supramolecular enzyme assays ranging from early examples with covalent chemosensors to the most recent applications of supramolecular tandem enzyme assays, which utilize common and often commercially available combinations of macrocyclic host molecules (e.g. cyclodextrins, calixarenes, and cucurbiturils) and fluorescent dyes as self-assembled reporter pairs for assaying enzyme activity.


Introduction
The determination of enzyme activity is a cornerstone in numerous research and application areas. For example, enzyme assays are widely used in clinical diagnosis, drug development, environmental pollution detection, and chemical biological research. [1][2][3] Optical spectroscopic methods and, in particular, uorescence-based methods are by far the most popular, because they are more sustainable than radioactive assays, enable a rapid and highly sensitive signal read-out within a short time, and allow continuous monitoring. 4 Moreover, uorescence-based assays can be easily miniaturized, which is especially useful for drug discovery by high-throughput screening (HTS) in the pharmaceutical industry, where large libraries of synthetic compounds are screened for their potential to serve as a drug lead structure against a specic biological target. [5][6][7][8][9][10] Mohamed Nilam graduated from the University of Colombo (Sri Lanka). He then moved to Jacobs University Bremen (Germany) to obtain a PhD in Chemistry under the supervision of Prof. Werner M. Nau. Presently, he is a postdoc in the group of Prof. Andreas Hennig at the Universität Osnabrück (Germany). His research interests are supramolecular photochemistry, supramolecular enzyme assay development, as well as liposome-based supramolecular sensors. Andreas Hennig graduated from TU Braunschweig (Dipl.-Chem.) and Jacobs University Bremen (PhD), and has subsequently held positions at the University of Geneva, BAM Federal Institute of Materials Research and Testing, and Jacobs University Bremen. Since 2020, he is Professor for Organic and Supramolecular Chemistry at the Universität Osnabrück (Germany). He has a keen interest in functional supramolecular systems that reliably work in complex environments such as biouids, membranes, cells, and on nanomaterial surfaces.
Oen, uorogenic substrates are used, which generate a uorescence response, for example, aer cleavage by proteases or other hydrolases. [11][12][13][14][15][16][17] For other types of enzymes, uoroimmunoassays with uorescently labelled antigens and antibodies are used (Fig. 1), in which a uorescently labelled antigen is displaced from an antibody binding site by the labelfree product of an enzymatic reaction (Fig. 1). 2,18 Fluorescence assays are thus an established technology for high-throughput screening application (HTS), 19 in drug discovery, clinical diagnostics, 20,21 and cancer-biomarker. 22,23 Nonetheless, these approaches have also certain disadvantages. Fluoroimmunoassays comprise oen heterogeneous assays with several incubation and washing steps and the exchange kinetics of antibodies are oen too slow to allow continuous monitoring, even in a homogenous assay format. Moreover, uorogenic substrates contain large aromatic dyes, which can interfere with antigen-antibody binding or with the binding of the substrate to the enzyme active site. 2,12,15 It has been shown in the last een years, that articial, supramolecular receptors can complement existing enzyme assay technologies. Such supramolecular approaches to enzyme assays rely on supramolecular chemosensors, which are capable to transform chemical information into useful signals and which have found wide-spread use for the detection of biomolecules. 24,25 The main assets of supramolecular chemosensors include the negligible large-scale production costs compared to antibodies, a much faster exchange kinetics compared to antibodies, and that they offer complementary molecular recognition principles. For example, supramolecular host molecules are designed to bind small, low-molecular weight guests, whereas it is usually rather difficult to raise specic antibodies against small, abundant biomolecules such as amino acids. In addition, supramolecular chemosensors can benetcounterintuitivelyfrom a lower selectivity compared to antibodies, because this allows to monitor various related enzymatic transformation with the same chemosensor; typically, a supramolecular chemosensor that discriminates substrate and product is sufficient. 26 Supramolecular approaches to enzyme assays present nowadays an attractive alternative to established enzyme assays and enable a label-free, continuous uorescence monitoring of an increasing number of enzymatic reactions.
The aim of this review is to summarize the current state-ofthe art in supramolecular approaches to enzyme assays. We provide an introduction to initial efforts from the supramolecular chemistry community to combine supramolecular chemosensing principles with the detection of enzymatic activity (Sections 2 and 3) and then summarize the foundations of "supramolecular tandem enzyme assays", which involve the use of uorescent supramolecular host-dye reporter pairs in detecting enzymatic conversions of small substrates (Section 4) and peptides and proteins (Section 5). In the nal part of the review (Section 6), we present potential applications of supramolecular enzyme assays, which include, for example, high-throughput screening (HTS) drug discovery, clinical diagnostics, and molecular imaging.

Enzyme assays with synthetic chemosensors
One of the rst uorescent chemosensors was developed in 1867 by F. Goppelsröder to detect Al 3+ , which forms a strongly uorescent chelate with morin. 27 This prompted the subsequent development of innumerable different uorescent chemosensors. Supramolecular chemosensors are commonly based on host-guest complexation, in which the analytes reversibly bind with the chemosensors. An elegant and very versatile approach to supramolecular chemosensing is the "receptor-spacer-reporter" approach ( Fig. 2), which was initially developed by de Silva and co-workers and could be successfully applied to detect cations, anions, small neutral molecules, and biomacromolecules. 28 The "receptor-spacer-reporter" design is based on three components: a receptor site that reversibly binds a particular analyte, a chromophore or uorophore that serves as a signalling unit, and a spacer, which connects the receptor and reporter within the same compound. For example, an aza-crown ether can be connected to the uorophore, which is able to alter the optical properties of the uorophore by quenching the uorescence through photoinduced electron transfer (PET). 29 Binding of the analyte, e.g. a sodium cation, changes the frontier orbital energy levels of the aza-crown ether and thereby prevents quenching by PET; this leads to a turn-ON uorescence response of the chemosensor. This system was further extended to other metal ions such as potassium and calcium. [29][30][31] Nowadays, it is integrated into point-of-care devices used to assess the electrolyte concentration in blood serum samples in both humans and animals. 32  Chemosensors based on the receptor-spacer-reporter approach have been also early on explored for assaying enzyme activity. Czarnik and co-workers synthesized the anthracenebased chemosensor 1 containing polyammonium groups as receptor unit, which allows cooperative chelation and uorescence detection of pyrophosphate but not monophosphate. 33 This selectivity was applied to monitor the hydrolysis of pyrophosphate into monophosphate by the enzyme pyrophosphatase (Fig. 3).
More recently, an alternative chemosensor to monitor pyrophosphatase activity was reported by Jolliffe and co-workers, who attached two Zn 2+ -dipicolylamino (DPA) units as a recognition motif and a coumarin dye as a reporter unit to a cyclic peptide scaffold to afford chemosensor 2. 34 The chemosensor showed a high selectivity and affinity for pyrophosphate. In its unbound state, 2 showed a comparably low uorescence intensity (potentially due to intramolecular association of the catechol unit in the coumarin with the Zn 2+ -DPA units), whereas addition of pyrophosphate led to an increase in uorescence intensity, implying intramolecular displacement of coumarin from Zn 2+ -DPA units upon pyrophosphate binding. The potential application of 2 in an enzyme assay was demonstrated through monitoring of pyrophosphatase activity. Introduction of pyrophosphatase into the reaction mixture containing 2 and pyrophosphate, resulted in hydrolysis of pyrophosphate into monophosphate. The latter binds much more weakly to 2 such that coumarin can again bind with Zn 2+ -DPA units of 2. This afforded a decrease in uorescence intensity in response to pyrophosphatase activity. Supramolecular chemosensors were also used to monitor phosphodiesterase (PDE) activity with nucleosides ( Fig. 4). 35 Kikuchi and co-workers synthesized 3, which includes 7-amino-4-triuoromethylcoumarin as a reporter uorophore and Cd 2+cyclen(1,4,7,10-tetraazacyclododecane) as an anion receptor unit. This chemosensor displayed micromolar affinity to nucleoside mono-, di-, and triphosphates, whereas cyclic adenosine monophosphate (cAMP) had a much weaker affinity to 3. Thus, the activity of PDE transforming cAMP to AMP can be monitored by receptor 3-Cd 2+ . Interestingly, the displacement of the axial amino group of the coumarin unit by the product AMP from the Cd 2+ -cyclen unit led to a red shi of the uorescence spectra enabling ratiometric monitoring, which was, however, not demonstrated.
In another study, 36 Hamachi and co-workers exploited the phosphate binding properties of Zn 2+ -DPA units to monitor the activity of glycosyltransferases with chemosensor 4 (Fig. 5). 4 binds phosphate monoesters more strongly than phosphate diesters and gives a uorescence increase upon binding, because PET is less effective in the phosphate complex. Glycosyltransfer could thus be monitored by the strong binding of the reaction product uridine 5 0 -diphosphate (UDP), aer transfer of the glycosyl residue from the glycosyl donor to the glycosyl acceptor. The biocompatibility of chemosensor 4 was conrmed by reproducing literature-known trends of a wide variety of enzyme kinetic parameters. This included combinations of different glycosyltransferases, namely b-1,4-galactosyltransferase, a-1,3galactosyltransferase, and a-2,3-sialyltransferase in combination with different substrates such as chitobiose, N-acetylglucosamine, glucose, N-acetyllactosamine, lactose, and galactose.

Enzyme assays by indicator displacement
In the preceding section, we have summarized methods to monitor enzyme activity using supramolecular receptors, which  The substrate cAMP does not bind to the Cd 2+ -cyclen unit, whereas the product AMP binds strong enough to displace the aminocoumarin dye from its axial position leading to a red-shift in fluorescence intensity.

Fig. 5
Chemosensor 4 binds nucleotide diphosphates more strongly than the glycosylated pyrophosphate diesters, which enables the monitoring of glycosyltransferase activity.
were covalently modied with a uorogenic or chromogenic reporter unit. The employed receptor-spacer-reporter strategy has, however, its limitations in the substantial synthetic effort to covalently connect the reporter dye and the receptor unit. An alternative strategy that bypasses such synthetic challenges is the indicator displacement assay (IDA), which was introduced into the supramolecular chemistry community by the group Eric V. Anslyn in the late 1990s to 2000s. 37,38 IDAs are dependent on indicator dyes that afford signicant absorption or uorescence spectral changes upon complexation with supramolecular receptor molecules. The addition of a competing guest molecule that can displace the dye from the receptor principle is nowadays routine to determine the affinity of supramolecular guest molecules not bearing a chromophoric or uorophoric unit by competitive titrations, [39][40][41] it has been used the elucidation of mechanistic pathways and for material characterizations, [42][43][44] and it was rst used to detect anion binding to cyclodextrins. 45 The conceptual advancement behind an IDA is the analytical application, namely that the competitor is an analyte that can to be detected or quantied based on the competition between the receptor/dye and receptor/analyte complex. 46 A sufficiently strongly binding analyte will displace the indicator at appropriate analyte concentrations resulting in a measurable optical output signal. The IDA-based sensing principle has been applied to a large variety of analytes, e.g. citrate, glucose-6phosphate, tartrate, malate, or nitrate. 47 The applied detection methods include absorption and uorescence spectroscopy.
As an example, the tripodal guanidinium-based receptor 5 ( Fig. 6) has been applied to assess the citrate concentration in various so drinks by uorogenic IDA. 47 The binding affinity between 5 and citrate was undetectably low in water, but in 25% (v/v) 5 mM Hepes, pH 7.4 in methanol, the binding affinity for 5 and citrate was found to be 2.9 Â 10 5 M À1 . Under these conditions 5-carboxyuorescein binds with mM molar affinity (K a ¼ 4.7 Â 10 3 M À1 ) to 5 leading to uorescence quenching. 38 The millimolar concentrations of citrate in so drinks thus led to displacement of 5-carboxyuorescein, which was detectable by UV-Vis and uorescence spectroscopy. Potential interferences of the sensor system include phosphate buffer 47 and succinate which has also a considerable binding affinity with 5 (K d ¼ 4.5 mM). 38,48 The earliest example of an IDA-based enzyme assay was reported by Reymond and co-worker, which used, quite unconventionally, metal ions as binding motifs for substrate and product discrimination (Fig. 7). 49 The quinacridone-derived uorescent dye 6 is strongly quenched by energy transfer to complexed Ni 2+ and Cu 2+ metal ions. The affinity of 6 to Ni 2+ and Cu 2+ was, however, comparably weak for a typical chelate complex, and bidentate ligands such as a-amino acids could efficiently compete for Ni 2+ and Cu 2+ binding with 6 leading to a recovery of the uorescence of unbound 6. The hydrolases acylase I and leucine aminopeptidase afford the a-amino acids L-methionine and L-leucine by hydrolysis of the substrates Nacetyl-L-methionine and L-leucinamide, respectively. Since the latter are incapable of coordinating to Ni 2+ or Cu 2+ , monitoring of acylase I and leucine aminopeptidase activity should therefore become possible. Unfortunately, continuous monitoring was only shown with signicant amounts of organic co-solvents (40% DMSO or 40% DMF), which was required to solubilize 6 and which reduced the activity of the enzymes. The native enzyme activity could, however, be determined in an aliquot assay, in which aliquots from the enzymatic reaction in water were successively withdrawn and diluted with the organic solvent containing water-insoluble 6.
The possibility to detect enzyme activity with absorptionbased IDAs was subsequently also shown by Anslyn and coworkers. 50 The receptor 7 has a high affinity to gluconic acid, which is the product of the enzymatic oxidation of glucose with glucose oxidase. Pyrocatechol violet (PV) was selected as the indicator dye, which forms a purplish-red coloured boronate ester with 7 ( Fig. 8). Also here, continuous monitoring was not possible, because the receptor 7 has insufficient water solubility and required 75% MeOH as an organic co-solvent, but an aliquot assay could be demonstrated, in which 250 mL aliquots were withdrawn from the enzyme assay mixture and diluted  The enzymatic hydrolysis of L-leucinamide by leucine aminopeptidase was followed with chemosensor 6. The reaction product is the a-amino acid L-leucine, which serves as a bidentate ligand for Cu 2+ and Ni 2+ and thus displaces M 2+ from 6. The hydrolysis of N-acetyl-Lmethionine into L-methionine by acylase I could be similarly followed. Fig. 8 A colorimetric, IDA-based enzyme assay for glucose oxidase. The self-assembled chemosensor 7 binds carboxy and phosphor sugars, but not aldose or ketose carbohydrates. Oxidation of glucose by glucose oxidase generates gluconic acid. Aliquots from the enzyme assay mixture were diluted with MeOH and the enzymatic reaction product displaced the indicator dye pyrocatechol violet (PV) from 7 leading to a colour change from red to yellow.
with 750 mL MeOH for detection. Within the progress of the enzyme reaction, the detection solution became successively yellowish-green, which is the colour of the unbound dye (Fig. 8).
An early example of an absorption-based, homogeneous assay format with the possibility for continuous monitoring of enzyme activity in solution was developed by Kim and coworkers. 51 They used the metal ion chelating properties of PV in conjunction with receptor 8 for a PDE assay (Fig. 9). 8 can also bind to the phosphate ester of AMP, but not to the phosphate diester of cAMP. Hydrolysis of cAMP by PDE into AMP continuously displaced the dye pyrocatechol violet generating an absorption change. Compared to commercial HTS assays, which are based on luminescence and can, thus, be easily adapted to microtiter plate, the chemoreceptor-based assay has a much lower sensitivity (<10 mM AMP) due to the absorptionbased measurement.

Supramolecular host-dye reporter pairs
Among various accessible methodologies for monitoring enzymatic activity, uorescence-based methods are usually preferable due to their sensitivity, short read-out times, and their possibility for continuous monitoring. 4 Prototypical classes of macrocyclic host molecules such as cucurbiturils, calixarenes, cavitands, cyclodextrins, pillararenes, and cyclophanes ( Fig. 10) are well-known to bind with a large variety of uorescent dyes (Fig. 11) and thereby alter their photophysical properties. 52,53 Typical effects include uorescence enhancement or quenching (Fig. 12), whereas other effects, such as changes in the uorescence lifetime and anisotropy have also been noted. [54][55][56] As a consequence, a large variety of host/dye complexes have been investigated in large detail with respect to their photophysical properties, 52 and certain host/dye combinations were also applied as chemosensors in ON- (Fig. 12a) or OFF-IDAs (Fig. 12b). 26 The best performing combinations of various hosts and dyes, which showed a large uorescence response and a high affinity, were summarized by Nau and co-workers, who also coined the name "reporter pair". 26 The reporter pairs have been used to assess the absolute concentrations of analytes as well as binding affinities to macrocycles. 57 Another requirement to label a host-dye combination as a reporter pair is a sufficiently fast exchange time. Typical exchange times of uorescent dyes with hosts are on the microsecond to millisecond time scale. 58 This is much faster than the reaction rates in typical enzyme assays, which are usually set up to proceed within minutes. Reporter pairs are thus ideally suited to follow dynamic changes in analyte concentration, in particular in response to an enzymatic reaction.

Product-selective supramolecular tandem enzyme assays
Supramolecular tandem enzyme assays present a broadly applicable approach for monitoring enzyme activity in a facile and accessible manner. The most intuitive way to set up a supramolecular tandem enzyme assay is in its product-  selective variant (Fig. 13a). 59 In a product-selective tandem enzyme assay, the substrate shows negligible affinity to the host and the product shows a very high affinity. The enzymatic reactions thus generate a strong competitor, which displaces the dye from the cavity leading to a pronounced uorescence response. This principle thus immediately affords a highly sensitive real-time uorescence monitoring of enzyme activity with label-free substrates.
Product-coupled tandem assays were initially developed for amino acid decarboxylases. 59 This class of enzymes removes the carboxylic acid group from amino acids leading to an overall increase of the positive charge and were thus monitored with   the cation receptors cucurbit [7]uril (CB7) and p-sulfonatocalix [4]arene (CX4) and dapoxyl and aminomethyl-substituted 2,3diazabicyclo[2.2.2]oct-2-ene (DBO) as uorescent dyes (see Fig.  10 for structures of hosts and Fig. 11 for structures of dyes). Both, CB7 and CX4 bind the zwitterionic amino acid substrates very weakly and the cationic decarboxylation products more strongly, but dapoxyl complexation by CB7 resulted in a uorescence increase, while CX4 forms a non-emissive inclusion complex with DBO. This resulted in an overall OFF-response for the CB7/dapoxyl-based assay (Fig. 13a) and an ON-response for the DBO/CX4-based assay (Fig. 13b). It is noteworthy, that the host does not need to be exceptionally selective to successfully set up a tandem assay, but that a relative selectivity to discriminate substrate and product oen suffices. The assay could be therefore universally applied to lysine, histidine, ornithine, tyrosine, and tryptophan decarboxylases, and the possibility to obtain time-resolved uorescent kinetic traces were employed to extract enzyme kinetic parameters such as the Michaelis-Menten constants (K m ). Subsequently, a rened variant of an ornithine decarboxylase assay was reported by us using cucurbit [6]uril (CB6) and trans-4-[4-(dimethylamino) styryl]-1-methylpyridinium iodide (DSMI) as a reporter pair, which was shown to be compatible with typical settings in HTS assays in the pharmaceutical industry (see Section 6). 60 Singaram and co-workers reported IDAs for selective sensing of carbohydrates and respective enzyme assays (Fig. 14). [61][62][63][64] The receptors 9 and 10 are based on phenylboronic acids attached to a viologen unit and 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) served as the indicator dye. The positively charged receptors bind with negatively charged HPTS through electrostatic interactions, which led to quenching of the HPTS uorescence. Sucrose phosphorylase (SPO) catalyses the transfer of the glucose residue of sucrose to orthophosphate resulting in glucose-1-phosphate and fructose. The receptor 9 binds fructose selectively such that the enzyme reaction product fructose displaces HPTS from 9 leading to a recovery of the HPTS uorescence in response to SPO activity. The constitutional isomer 10 can discriminate glucose-1-phosphate and glucose-6-phosphate, whereby the latter showed a stronger binding to 10. This enabled monitoring the isomerization reaction catalysed by phosphoglucomutase with the selfassembled 10/HPTS chemosensor.
The most recent example of a product-selective tandem enzyme assay involved the specic detection of steroids by CB7 and CB8. 65 It was found that nandrolone had nanomolar affinity to CB8, whereas the affinity of nandrolone 17-propionate was lower by a factor of ca. 5. Therefore, hydrolysis of the low affinity propionate ester by pig liver esterase (PLE) was monitored with a CB8-based chemosensor.

Substrate-selective supramolecular tandem enzyme assays
Product-selective tandem enzyme assays are very intuitive and easy to design, when the net charge is increased during the enzymatic reaction, because water-soluble macrocyclic receptors rely on weak interaction involving charges for selective binding (anion or cation receptors). 26 A change in the net charge of a molecule during an enzymatic reaction is very common, but many enzymatic reactions alter the net charge in the "wrong" direction for a product-selective assay. When the net charge of a molecule is decreased during the reaction, a host would be required that binds more strongly with a less charged product, which is actually very challenging to design. It thus appeared very desirable to establish "substrate-selective tandem assays", in which the substrate has a higher binding affinity to the macrocycle than the product (Fig. 15).
Fig. 14 Enzyme assay with self-assembled carbohydrate chemosensors 9 and 10 with HPTS as a fluorescent dye (see Fig. 11 for dye structure). Sucrose phosphorylase (SPO) activity was monitored with 9, which selectively binds fructose that is generated by glucosyl transfer from sucrose to orthophosphate. The chemosensor 10/HPTS binds glucose-6-phosphate stronger than glucose-1-phosphate, which enabled monitoring of phosphoglucomutase (PGM) activity by HTPS displacement from 10 by the product glucose-6-phosphate. Schematic representations of product-selective and substrate-selective tandem enzyme assays. In a product-selective tandem enzyme assay (top), the product binds more strongly and displaces the fluorescent dye during the course of the enzymatic reaction. In a substrate-selective tandem enzyme assay (bottom), the substrate binds more strongly and the dye can bind to the macrocycle when the substrate is converted into a weaker binding product. Thereby, it is important to consider that complexation of the substrate may affect the enzymatic reaction. This potentially detrimental influence can be reduced by selecting assay conditions, in which sufficient free substrate exists in solution and only a minor fraction actually displaces the dye from the receptor at the beginning of the reaction.
One of the important aspects of a substrate-selective tandem enzyme assay is that the substrate is complexed by the receptor and may, thus, not be recognized by the enzyme. In extreme cases, the enzymatic reaction may be even fully inhibited. 17,66 As a remedy, the conditions in a substrate-selective tandem enzyme assay may be chosen in such a way that only a small fraction of the substrate is actually bound (e.g. 10-20% of the total substrate concentration), whereas the major fraction of the substrate is uncomplexed and free in solution, where it can be accessed by the enzyme. The ideal condition for substrateselective assay is met, when the substrate binds more strongly to the receptor than the product, but not too strongly to be fully complexed. During the course of the enzymatic reaction, the dynamic substrate binding will constantly replenish free substrate in solution, which has been removed from the equilibrium by conversion into the product. 67 It is noteworthy that supramolecular tandem assays can be continuously monitored in the substrate-selective mode, which is commonly not possible with antibody-based enzyme assays. The reason is that antibodies have commonly much slower dissociation rates than supramolecular receptors. 68 To allow full equilibration of a competitive binding equilibrium with antibodies requires incubation times of several minutes to hours, which is much slower than the enzymatic reaction and prevents a continuous monitoring. In contrast, exchange of supramolecular host-guest complexes proceeds within microseconds to milliseconds, such that the enzymatic reaction is rate-limiting and not the establishment of the binding equilibrium.
Substrate-selective tandem enzyme assays were initially realized with two different enzymatic reactions: the hydrolysis of the guanidinium group of arginine into ornithine by arginase and the conversion of cadaverine into 5-aminopentanal by diamine oxidase (Fig. 16). 67 The CX4/DBO reporter pair was used for arginase and CB7/acridine orange (AO) was used for diamine oxidase. In both cases, the substrates had a higher affinity for the hosts than the products (6400 M À1 for arginine vs. 500 M À1 for ornithine and 4.5 Â 10 6 M À1 with cadaverine vs. 1.1 Â 10 5 M À1 with 5-aminopentanal), and the time-resolved uorescence traces showed the expected switch-OFF and switch-ON response aer enzyme-induced host-dye complex formation, respectively.
However, to extract meaningful enzyme kinetic parameters from uorescence progress curves in substrate-selective assays, several precautions need to be considered. For example, complex formation of substrates with host molecules were oen used to prevent undesirable enzymatic degradation, such that potential inhibitory effects of the host need to be considered. 66,69 Moreover, progress curves of substrate-selective tandem assay may show a lag phase, in which the substrate is converted into the product without any observable change in uorescence. This happens, when the concentrations of the reporter pair are too low for the selected substrate concentration, such that a large fraction of substrate remains in the reaction mixture although the enzymatic reaction has already signicantly progressed. These effects are, however, not detrimental for the determination of inhibition constants; once appropriate concentrations of substrate and reporter pair that give an immediate response have been identied, the inhibitory effect is reected in decreasing rates of initial uorescence changes, which can be used to reliably determine inhibition constants. A comprehensive guide for the optimization of tandem enzyme assays including a thermodynamic and kinetic analysis has been published previously. 26 It is interesting to note, that substrate-selective assays were subsequently more widely adapted by the supramolecular analytical chemistry community than the much simpler product-selective assays. For example, Nau and co-workers tested several reporter pairs with the goal to monitor the hydrolysis of ATP. 70 The reporter pairs included, among others, 2-anilinonaphtalene-6-sulfonate (ANS) in combination with octakis(6-deoxy-6-amino)-g-cyclodextrin and 8-hydroxy-1,3,6pyrene trisulfonate (HPTS) with a cyclophane (see Fig. 10 and 11 for structures). These anion receptors showed a higher affinity for the substrate ATP than for AMP, which enabled monitoring of potato apyrase activity in a switch-ON (amino-gcyclodextrin/ANS) and switch-OFF (cyclophane/HPTS) assay (Fig. 17). Moreover, the two reporter pairs were complementary with respect to the substrate affinity. With the high, micromolar affinity of amino-g-cyclodextrin, substrate concentrations below the Michaelis-Menten constant, K M , of potato apyrase could be used, which is ideal for screening activators and inhibitors, whereas the low, millimolar affinity of cyclophane allowed to use substrate concentrations much above the K M , which is ideal to assess the maximal initial rate, v max , and thus enzyme purity (e.g. in quality control in enzyme production). Enzyme activation could be successfully conrmed with divalent metal ions (Ni 2+ , Mg 2+ , Ca 2+ , and Mn 2+ ) and the extracted v max values were in agreement with literature-known initial reaction rates. (a) Diamine oxidase converts the doubly positively charged substrate cadaverine, which is strongly bound by the cation receptor CB7, into singly positively charged 5-aminopentanal, which has a weaker affinity. (b) Arginase hydrolyses the guanidinium group of arginine to afford the non-proteinogenic amino acid ornithine. The latter has a weaker affinity to CX4 as a supramolecular receptor.
Finally, the transferability to other nucleotide triphosphates (GTP, TTP and CTP) was also demonstrated.
A related calixpyridinium-based tandem assay for alkaline phosphatase (ALP) was reported by Kui Wang and co-workers. 71 Calixpyridinium, a positively charged water soluble macrocyclic host, which is bind with 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt (PTSA) led to a uorescence quenching. Also, ATP and AMP have different binding affinity with calixpyridinium. Enzymatic transform of ATP into AMP can be easily monitored by calixpyridinium/PTSA reporter pair (Fig. 18), because substrate and product have different binding affinity to macrocycle. Addition of calf intestinal alkaline phosphatase (CIAP) into the reaction mixture containing calixpyridinium/PTSA and ATP, in which CIAP catalysis hydrolysis of ATP, resulted in decrease in uorescence due to displacement of product (AMP) by the high affinity dye. Stepwise dephosphorylation with ADP as an intermediate unable to follow by the host calixpyridinium because the substrate ATP and intermediate product ADP have almost same binding affinity to the calixpyridinium (K a ¼ ca. 1 Â 10 4 M À1 ). The dependence of the initial reaction rate on varying substrate concentration experiments were carried out below the K m value of the enzyme, and showed typical linear dependence of initial reaction rates on the substrate concentration. Also, Lineweaver-Burk plot gives K m of (105 AE 21) mM.
ALP is known to have a broad substrate tolerance and thus cleaves many different phosphate ester bonds. Guo and coworkers could thereby monitor the dephosphorylation of pyridoxal-5 0 -phosphate (PLP) into pyridoxal and phosphate by ALP. 72 The calix [5]arenes of the Guo group show very interesting molecular recognition properties. 39,73,74 and a guanidinocalix [5] arene (G-CX5, see Fig. 10) bound uorescein (FL) with micromolar affinity (K a ¼ 7.0 AE 2.1) Â 10 6 M À1 and strong uorescence quenching. This afforded G-CX5/FL as a new reporter pair for anions, which was able to bind PLP with micromolar affinity (K a ¼ 2.0 AE 0.5) Â 10 6 M À1 , whereas phosphate has millimolar affinity (K a ¼ 7.9 AE 0.9) Â 10 4 M À1 with G-CX5. Addition of ALP to a mixture of PLP and the reporter pair gave the expected switch-off uorescence response and the initial reaction rates were found to be linearly dependent on the initial PLP concentration. This was used for the quantication of PLP with an excellent limit of detection of (26.5 AE 0.6) nM.
Further substrate-selective tandem enzyme assays were developed for choline oxidase (CO) by Jie Yang and co-workers 75 and by Guo and Liu for butyrylcholinesterase (BChE) 76 by using cation receptors (Fig. 19). The group of Jie Yang showed for the rst time the usefulness of pillararenes in tandem enzyme assays. 75 The pillar [6]arene WP6 (see Fig. 10) formed a suitable reporter pair with AO resulting in uorescent quenching and a colour change from yellow to red indicative of charge-transfer interactions. Choline showed a higher binding affinity (K a ¼ (5.26 AE 0.36) Â 10 4 M À1 ) than the choline oxidase product betaine, which afforded a product-selective switch-OFF tandem assay. Guo and Liu selected several sulfonated calixarenes based on the known recognition motif of the trimethylalkylammonium group of cholines for the possibility to discriminate succinylcholine and choline. 76 They identied CX4 as the most suitable host and combined it with lucigenin (LCG, Fig. 11) to form the strongly quenched CX4/LCG reporter pair. CX4 was found to bind succinylcholine with low micromolar affinity and with 10 fold higher affinity than choline. Since BChE also hydrolyses succinylcholine into choline, BChE activity could be followed allowing even the determination of the K M value of BChE despite the limited range of accessible substrate concentrations.
Very recently, a supramolecular tandem enzyme assay for avin monooxygenase 3 (FMO3) has been reported. 77 The enzyme FMO3 oxidizes the amine of trimethylamine (TMA) to afford trimethylamine N-oxide (TMAO), which is a signicant risk factor in cardiovascular thrombotic events. It was found that the substrate TMA has a higher binding affinity to CX4 than the product TMAO, which suggested the use of a CX4-based reporter pair. Several dyes, namely LCG, methylene blue, pyronine, and oxazine 1 (OX1) were screened for their compatibility with the assay conditions, among which OX1 (see Fig. 11 for dye Fig. 17 Substrate-selective tandem enzyme assay for monitoring ATP-dependent enzymes. The positively charged octakis(6-deoxy-6amino)-g-cyclodextrin binds the more negatively charged ATP more strongly than AMP, which enables monitoring of the hydrolysis of ATP by potato apyrase. Fig. 18 Substrate-selective tandem enzyme assays for calf intestinal alkaline phosphatase (CIAP) using calixpyridinium/PTSA as reporter pair. CIAP hydrolyses ATP, which binds strongly to the positively charged calixpyridinium receptor, into uncharged adenosine, which shows no interaction. The fluorescent dye PTSA can subsequently bind to the calixpyridinium receptor affording a fluorescence decrease during the course of the enzymatic reaction. structure) was found to be most tolerant towards the presence of NADPH and NADP + as well as TMAO. In the CX4/OX1 reporter pair, the uorescence of OX1 was quenched by a factor of ca. 5 and the binding constant was sufficiently high (1.4 Â 10 4 M À1 ). Consequently, the uorescence is decreased in the course of enzymatic reaction, because the consumption of TMA enabled the re-encapsulation of OX1 by CX4. This has been used to identify a series of FMO3 inhibitors from a traditional Chinese medicine composed of eleven herbs with known antithrombotic activity (see Section 6.1).

Enzyme-coupled assays and domino assays
In many enzyme assays and biosensors, more than one enzyme is used. This strategy is classically applied when it is difficult to obtain an output signal from one of the enzymatic reactions. For example, peptidylprolyl isomerases (PPIases) are enzymes, which catalyze the cis-trans isomerization surrounding the proline amide bond (Fig. 20a) and which play important roles in protein folding. 78 Such isomerization reactions are notoriously difficult to monitor by absorption or uorescence spectroscopy. However, the protease chymotrypsin has a much higher catalytic efficiency for the cis form relative to the trans form of the proline bond and hydrolysis of peptide bonds are particularly easy to monitor by spectroscopy. 79 The consequence is to set up an enzyme-coupled enzyme assay, in which the isomerization reaction is rate-limiting, such that every peptide with a trans proline amide bond is immediately cleaved resulting in a spectroscopic signal change. The remaining cis prolines are then converted into trans proline by PPIase as a rate-limiting step and the signal change from hydrolysis directly reects the activity of PPIase.
Another popular strategy is coupling an enzymatic reaction that is difficult to monitor with NAD(P)H-dependent dehydrogenases. The reduction of NAD(P) + into NAD(P)H and vice versa can be easily monitored by the absorption of NAD(P)H at 340 nm (3 ¼ 6300 M À1 cm À1 ). 80 This enables, for example, to monitor the reaction of hexokinase by converting the hexokinase product glucose-6-phosphate into gluconate-6-phosphate by the NADP + -dependent glucose-6-phosphate dehydrogenase and the increase in absorption due to formation of NADH (Fig. 20b). 81 Kinetic analysis of the enzyme progress curves may become quite involved, when the enzyme-coupled enzyme assays cannot be ideally set up due to limitations in the enzyme kinetic parameters of the involved enzymes, or when the enzyme used for detection is not available in sufficiently large quantities to be used in excess amounts. However, generation of a measurable optical output signal is commonly unproblematic regardless of the actual enzyme kinetic parameters and concentrations. 81 The versatility of enzyme-coupled enzyme assays is also very attractive to expand the scope of supramolecular tandem enzyme assays. The use of multistep enzymatic reactions in tandem assays was shown with an elegant model system based on the CX4/LCG reporter pair (Fig. 21). 82 CX4 is incapable of Fig. 19 Substrate-selective supramolecular tandem enzyme assays measuring (a) choline oxidase activity with a per-carboxylated pillar [6] arene and AO as reporter pair (b) butyrylcholinesterase activity based on CX4/LCG as reporter pair. In both cases, the host is occupied by the substrate and the dye is fluorescent free in solution. After conversion of the substrate, the low-affinity product cannot displace the dye leading to complex formation and quenching.  (1) is monitored by oxidizing the product glucose-6-phosphate with an excess of the NADP + -dependent glucose-6-phosphate dehydrogenases (2). The conversion of NADP + into NADPH is followed by an increase in the absorbance at 340 nm. discriminating between acetylcholine and choline, which prevents its use in a tandem assay for acetylcholinesterase (AChE). Therefore, an enzyme-coupled tandem assay was conceived using the transformation of choline to betaine by choline oxidase. The cation-responsive reporter CX4/LCG was able to distinguish between choline and betaine due to the different charge status of these two molecules. While choline is cationic, betaine is zwitterionic and has a much lower binding affinity to the cation receptor CX4. With an excess amount of choline oxidase, the enzymatic reaction of AChE became ratelimiting. This allowed the determination of enzyme kinetic parameters of AChE, which showed literature-known trends.
An interesting way to combine two enzymatic reactions and a reporter pair has been coined "domino-tandem assay", in which a two sequential enzymatic reactions and a single reporter pair afforded an ON-OFF-ON signal pattern (Fig. 22). 67 Essentially, the demonstrated domino assay is a sequence of a product-selective and a substrate-selective assay and was realized with the CB7/AO reporter pair and the enzymes lysine decarboxylase and diamine oxidase. In detail, lysine is a weak competitor and the CB7/AO complex renders the mixture brightly uorescent. Addition of lysine decarboxylase gives cadaverine and the uorescence intensity decreases signicantly, because the strong competitor cadaverine occupies the CB7 cavity. The sequential conversion of cadaverine into the weak competitor 5-aminopentanal by addition of diamine oxidase allows re-formation of the CB7/AO complex and the bright uorescence is recovered.

Tandem enzyme assays using associative binding
An alternative to indicator displacement is the construction of self-assembled chemosensors using ternary complexes composed of a macrocyclic host molecule, an optically active rst guest (e.g. a uorescent dye), and an analyte as a second guest (Fig. 23). 83 For example, the cavity of CB8 is sufficiently large to accommodate two aromatic guest molecules to form a stable, ternary complex stabilized by aromatic p-electron donor-acceptor or charge transfer interactions within the hydrophobic cavity of CB8. 84 The spectroscopic signal change immediately suggests its application as a chemosensor, for example with electron-poor methyl viologen for electron-rich naphthols and indoles as analytes, but the signal change was too weak for chemosensing. 85,86 To enhance the sensitivity of associative binding assays electron-poor uorescent dyes were found to be useful for the detection of electron-rich guests. In particular, inclusion of 2,7dimethyldiazapyrenium (MDAP) by CB8 retained the bright uorescence of MDAP, whereas binding of the analyte to the preformed CB8/MDAP complex resulted in strong uorescence quenching of MDAP. This was successfully applied in homogeneous solution to the detection of a large variety of electronrich aromatic guests, e.g. alkoxynaphthalenes, indole derivatives including the neurotransmitter tryptamine, and Nterminal phenylalanine residues in peptides. 40,83,87,88 Because of the rapid complex formation and dissociation kinetics of CB8 ternary complexes, associative binding is also attractive for real-time monitoring of enzymatic reactions ( Fig. 24 and 25). 83 Oxidoreductase activity (Fig. 25a) included the oxidation of various phenols, catechol, b-naphthol, aniline, and tryptophan by horseradish peroxidase (HRP), oxidation of tryptophan by lactoperoxidase (LPO), oxidation of 4-chlorophenol by laccase, and oxidation of thioanisole by chloroperoxidase (CPO). These reactions were successfully monitored with the CB8/MDAP chemosensor (except tryptophan and LPO, which was monitored with CB8/MDPP). Since the CB8/MDAP sensor was not affected by the presence of the co-substrates NAD + or NADH, oxidation of p-methoxybenzyl alcohol and reduction of benzaldehyde by NADH/NAD + -dependent alcohol dehydrogenase (ADH) could be followed as well. In selected   cases, the reactions were also monitored with CB8 complexes of perylenediimides (PDI) and dimethyldiazaperopyrenium (MDPP; see Fig. 11 for structures) as dyes, which afforded similar kinetic proles and suggested that the chemosensors do not detrimentally affect the enzyme activities. The usefulness of associative tandem enzyme assays for enzyme activity screenings was also demonstrated with HRP and varying 4-substituted phenols as substrates, where more electron-rich phenols were oxidized more rapidly (Fig. 25a: R ¼ -OMe > -Me > -H > -F), and by the pH dependence of tryptophan oxidation by HRP, which was consistent with the formation of different chemical intermediates at different pH. 89 Moreover, the accelerated oxidation of chlorophenol in the presence of 2,2 0 -azino-bis(3ethylbenzothiazoline-6-sulphonic acid) (ABTS) or N-hydroxyphthalimide as redox mediators was successfully reproduced.
Apart from redox reactions, numerous enzymatic hydrolysis reactions were also monitored by associative binding. This included detection of pig liver esterase (PLE) with phenyl acetate, alkaline phosphatase (ALP) with phenyl and naphthyl phosphate, hydrolysis of the b-lactam ring of penicillin G by penicillinase, and hydrolysis of phenyl-b-D-galactopyranoside by b-galactosidase (b-Gal) with CB8/MDAP (Fig. 25b). The exopeptidase activities of carboxypeptidase A and leucine aminopeptidase (LAP) and the endopeptidase activity of pepsin could be monitored by CB8/MDPP, which is described in more detail in Section 5.1.
Overall, the wealth of different enzymatic reactions investigated with associative reporter pairs demonstrated the versatility of this new enzyme assay platform. It is interesting to note, that the substrate and product affinities as well as the quenching efficiencies have not been investigated in detail for all reported enzyme activities. Although it transpired from the uorescence progress curves of the enzymatic reactions that more electron-rich second guests were more efficiently bound and/or quenched by the electron-poor CB8/dye chemosensors, it is interesting to conceive that tandem assays based on associative binding could be also potentially realized when substrate and product have the same affinity (K a (S) ¼ K a (P)), but show different spectroscopic responses, for example a different quenching efficiency of the ternary host/dye/substrate and host/ dye/product complexes.

Supramolecular enzyme assays with peptides and proteins
In the preceding sections, we have introduced the various concepts, which enable the monitoring of enzymatic reactions with label-free substrates by using supramolecular receptors. From the viewpoint of enzyme assay development, some of the investigated enzymes are easy to monitor with other types of assays. This includes, for example, unspecic hydrolases such as ALP, PLE, or b-Gal, for which numerous uorogenic and chromogenic substrates are commercially available. However, the simplicity of supramolecular enzyme assays is unmet by other assays for other types of enzymes. For example, amino acid decarboxylases have a very narrow substrate specicity and easily distinguish homologues of amino acids (e.g. ornithine and lysine). Consequently, decarboxylases do not tolerate major substrate modications such as the introduction of chromogenic or uorogenic dyes. 90 In principle, antibody-antigen recognition can also be used to detect label-free enzyme reaction products or substrates, 19 but it is particularly challenging to raise antibodies against low-molecular weight compounds such as amino acids. As a consequence, label-free supramolecular enzyme assays present an attractive alternative to conventional enzyme assays.

Supramolecular protease assays
As mentioned above, many unspecic hydrolases are easy to monitor, because uorogenic or chromogenic residues can be easily introduced without losing substrate specicity. However, many other peptidases and proteases have a much narrower substrate specicity and require extended amino acid sequences for successful substrate recognition. 11,91 The most common approach to assess these peptidases and proteases involves double-labelling of the peptide recognition sequence at remote sites. This may become synthetically cumbersome and is not applicable to exoproteases such as carboxy-and aminopeptidases (see below), which cleave off a natural C-terminal or N-terminal amino acid. [11][12][13] But even if double-labelling can be performed, some proteases such as clostridial neurotoxins are very sensitive towards substrate labelling and the substrate activity is largely sacriced by synthetic modications. 92,93 Supramolecular enzyme assays can therefore also present an attractive, label-free alternative for peptide-based substrates; the prerequisite is that the supramolecular receptor can distinguish between the intact peptide and the peptide fragments aer hydrolysis. Peptide recognition motifs have been explored for numerous macrocyclic host molecules including calixarenes and cucurbiturils. 94,95 For example, the amino acid phenylalanine has only moderate affinity to CB7 as an internal peptide residue (ca. 10 4 M À1 ), whereas an N-terminal Phe binds much more strongly due to additional ion-dipole interactions with the positively charged ammonium group (K a > 10 6 M À1 ). 96,97 This differential binding affinity has been exploited in a label-free enzyme assay for thermolysin (Fig. 26a). 98 Thermolysin is an endoprotease, which cleaves the amide bond at the a-nitrogen atom of Leu and Phe residues and liberates a peptide with an N-terminal, positively charged Leu or Phe residue. Overall, the binding affinity of six different enkephalin-type peptide substrates (Fig. 26a, 1-6) and their respective cleavage products (Fig. 26a, 7-9) to CB7 was investigated, which revealed a lower affinity of the substrates (K a ca. 10 4 M À1 ) compared to the products (K a > 10 7 M À1 ) and allowed to monitor substrate cleavage in a product-selective, label-free enzyme assay with the reporter pair CB7/AO. Interestingly, the binding affinity determinations revealed a different affinity of the diastereomeric substrates 1 and 2 with L-and D-Ala residues in the P1 position with CB7. Such a diastereodifferentiation of dipeptides was previously noted for Phe-Leu with L-Leu and D-Leu. 99 With respect to enzyme speci-city, substrate 1 with an L-Ala residue was rapidly hydrolysed, whereas cleavage of peptide 2 with D-Ala was insignicant, which is in accordance with the known specicity of thermolysin. 100 Even more interesting, a previously unreported exopeptidase activity of thermolysin has been discovered in the course of developing the supramolecular tandem assay. According to the reported substrate specicity of thermolysin, cleavage of substrate 3 should only occur at the Ala-Phe peptide bond, but not at the Phe-Leu bond, because the latter would involve cleavage of a C-terminal amino acid and, thus, exopeptidase activity. However, dye displacement from CB7 was much lower than expected for the product Phe-Leu-NH 2 , which prompted a more thorough investigation of the cleavage products by mass spectrometry. This revealed formation of H-Thr-Gly-Ala-Phe-OH as a product, which clearly showed that thermolysin can cleave C-terminal Leu and, thus, act as an exopeptidase. Noteworthy, such exopeptidase activities of endopeptidases remain undetectable with conventional doublelabelling approaches in protease assays. 11 Another interesting observation was an ON-OFF-ON signal modulation, when the peptide H-Leu-Ser-Arg-Phe-Ser-Trp-Gly-Ala-OH was cleaved by LAP in the presence of the reporter pair CB7/AO (Fig. 26b). 101 LAP is an aminopeptidase with relatively broad substrate specicity and thus successively cleaves off N-terminal amino acid residues. Aer initial cleavage of several amino acid residues, the peptide fragment H-Phe-Ser-Trp-Gly-Ala-OH is formed. The N-terminal phenylalanine residue strongly binds to CB7 and gives the switch-OFF uorescence response by AO displacement. Subsequently, LAP continues the hydrolysis and also cleaves off the N-terminal phenylalanine residue to afford H-Ser-Trp-Gly-Ala-OH. The low binding affinity of this peptide allows re-formation of the CB7/AO complex, which is indicated by a uorescence switch-ON response. Reminiscent of the tandem domino assays (see Section 4.4), this signal generation pattern presents a combination of product-selective and substrate-selective molecular recognition of the peptide fragments generated by the same enzyme. The proposed mechanism was conrmed by detecting the respective peptide fragments by mass spectrometry (ESI-MS). In contrast, digestion of the substrate by trypsin yielded the product H-Phe-Ser-Trp-Gly-Ala-OH and the concomitant switch-OFF response without a subsequent regeneration of the uorescence intensity.
Recognition of N-terminal Phe residues was subsequently exploited by Yong Bi and co-workers to develop an enzyme assay for dipeptidyl peptidase 4 (DPP4) based on CB7/AO (Fig. 26c). 102 DPP4 cleaves off dipeptides from the N-terminus of peptides or small proteins (below 80-100 residues) with a preference for proline or alanine in the S1 position of the cleaved peptide bond. 103 The tetrapeptide H-Gly-Pro-Phe-Gly-OH is an established substrate, which yields H-Phe-Gly-OH aer enzymatic cleavage. The latter has a stronger binding affinity to CB7 than the substrate or the other cleavage fragment H-Gly-Pro-OH due to liberated N-terminal Phe, which results in a turn-OFF uorescence response due to AO displacement. In addition, another two tetrapeptides were investigated, namely H-Gly-Pro-Gly-Gly-OH and H-Gly-Pro-Gly-Phe-OH. The results revealed that neither the substrate nor the product showed considerable binding with CB7 due to the lack of an N-terminal aromatic amino acid, which is required for strong binding with CB7. The authors demonstrated the potential of the assay for HTS applications and miniaturized the system with microplates. This allowed the investigation of enzyme kinetics, which indicated a linear dependence on the enzyme concentration and gave K m ¼ 282.6 mM by a Lineweaver-Burk plot. Also, a limit of detection (LOD) of 18.6 ng mL À1 was found for DPP4 and the specicity of the assay was conrmed with a-glucosidase, bovine serum albumin (BSA), lipase, a-chymotrypsin, carboxypeptidase Y and trypsin, which indicated a high selectivity of the assay towards DPP4.
The rst supramolecular protease assay using calixarenes as host molecules was reported by the group of Dong-Sheng Guo. 73 They found that insulin has mM affinity to the macrocycle CX4, which was exploited in a protease assay for pepsin using the CX4/LCG reporter pair. The peptide fragments resulting from the enzymatic cleavage by pepsin had a lower affinity than the substrate insulin resulting in a substrate-selective assay with a uorescence switch-OFF response. The dependence of the initial reaction rate on substrate concentration was also investigated and gave a linear Lineweaver-Burk plot with a K m value of (1.4 AE 0.3) mM. This assay may be useful in clinical diagnostics (see Section 6.2). Supramolecular tandem protease assays were also shown based on associative binding with the CB8/MDPP reporter pair (Fig. 27). 83 This included the exoproteases leucine aminopeptidase (LAP) and carboxypeptidase A (CPA) and the endoprotease pepsin. The CB8/MDPP reporter pair was used to monitor Cterminal hydrolysis of hippuryl-L-phenylalanine (¼N-benzoyl-Gly-Phe-OH) by CPA and N-terminal hydrolysis of H-Ala-Phe-OH, H-Trp-Gly-Gly-OH, H-Gly-Trp-Gly-OH, and H-Trp-Leu 6 -H by LAP. As noted in Section 4.5, quenching efficiencies and binding affinities were not always claried, but it can be assumed that the free aromatic amino acids phenylalanine and tryptophan had a higher binding affinity to the macrocycle CB8/ MDPP complex than the amino acid side chains in the peptides. Ternary complex formation with CB8/MDPP reduced the uorescence intensity of MDPP in response to the enzymatic reaction. As another demonstration, most proteins contains aromatic amino acids inside the protein hydrophobic core, which are thus unavailable for direct interaction with chemosensor due to steric shielding. Addition of relatively unspecic peptidases (e.g. pepsin) into a solution containing protein (e.g. bovine serum albumin (BSA)) lead to the degradation of the protein and loss of the its tertiary structure. The aromatic amino acids, which were previously in the hydrophobic core, can then interact with the chemosensor. This has been shown with BSA and pepsin at pH 2 in the presence of CB8/MDPP.

Supramolecular methyltransferase and demethylase assays
Calixarenes are well-known for their high affinity towards trimethylalkylammonium groups (see the AChE assay in Section 4.4). This molecular recognition pattern also prompted the use of calixarene-based reporter pairs for an enzyme assay for histone lysine methyltransferases (HKMTs). 104 HKMTs are responsible for methylation of lysine residues at particular sites of histone tails using (S)-adenosyl-L-methionine as a methyl donor (Fig. 28) and the HKMT class Dim-5 gives specically trimethylated lysine residues at histone tails.
The binding constants of various methylated lysine derivatives increased from K a < 10 3 M À1 for Lys to K a ¼ 1.3 Â 10 5 M À1 for trimethylated lysine, such that an increase in the binding affinity was expected upon HKMT activity. The supramolecular tandem enzyme assay with the CX4/LCG reporter pair provided a switch-ON uorescence response consistent with a higher affinity of the trimethylated product compared to the substrate containing an unmodied lysine residue. In an effort to determine enzyme kinetic parameters, the expected linear dependence of the initial reaction rates on varying enzyme concentrations at constant substrate concentrations was conrmed. However, the substrate concentration could only be varied within a narrow range and no enzyme kinetic parameters could be extracted. Within ca. 1 to 5 mM substrate concentration, an approximately linear dependence of the initial reaction rates suggested that the substrate concentrations were below the K M of the enzyme.
Demethylation reactions of lysine side chains in peptides were subsequently explored by Hooley and co-workers with the histone demethylase JMJD2E, which catalyses the demethylation of a histone H3 peptide fragment at lysine residue 9 of the substrate H3K9Me 3 (peptide sequence: Ala-Arg-Thr-Lys-Gln-Thr-Ala-Arg-Lys(Me 3 )-Ser-Thr-Gly-Gly-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Ala) (Fig. 29). 105 They used cavitand 1, which is also known to bind trimethylalkylammonium ions, and the uorescent dye FAMR as reporter pair (see Fig. 10 and 11 for structures). Binding of the reporter pair was based on the anchor group strategy, 106,107 but actual quenching occurred due to aggregation of the FAMR/cavitand 1 complex. Binding titrations gave a dissociation constant of K d ¼ (17 AE 10) mM for FAMR/cavitand 1 and addition of H3K9Me 3 to the reporter pair led to uorescence enhancement due to the displacement of the dye from the cavity of cavitand 1 and concomitant deaggregation. When JMJD2E was added, a continuous decrease in uorescence was observed, which originated from demethylation of the lysine residue in H3K9Me 3 to afford the product H3K9.
In an extension of this work, site-selective post-translational modications in H3 peptides at lysine residues in positions 4, 9, and 27 (H3K4Me 3 , H3K9Me 3 , H3K27Me 3 ) could be distinguished. 108 Therefore, a receptor array of cavitands 1 to 3 ( Fig. 10) with FAMR (Fig. 11) as a dye was used in 96-well microtiter plates. The resulting uorescence response was analysed by principal components analysis (PCA), which clearly indicated that the sensor array can discriminate the trimethylation in the different positions. The sensor array could also be used to conrm the site-selectivity of the JMJD2E demethylase and the lysine methyltransferase PRDM9.

Supramolecular kinase and phosphatase assays
Phosphatases and, in particular, kinases are important enzyme classes, which are oen targeted in HTS applications in drug discovery. 14 One possibility to monitor kinase activity is the use of a reporter pair that can discriminate the kinase co-substrate ATP from ADP that results from the transfer of the phosphate group (see Section 4.3). However, kinase and phosphatase assays can also be realized involving supramolecular reporter pairs, which discriminate phosphorylated and unphosphorylated peptides. 109 This was shown with the two cationic peptides P1 (H-LRRWSLG-OH) and P2 (H-WKRTLRRL-OH), which bind with micromolar affinity to CX4 and efficiently displace LCG from the CX4/LCG reporter pair (Fig. 30a). The respective peptides, which were phosphorylated at the serine and threonine residue, had a much lower affinity, because the phosphate group reduces the overall positive charge of the peptides and, thus, decreases the binding affinity with the cation receptor CX4 signicantly. Enzymatic phosphorylation of P1 and inhibition by N-ethylmaleimide was nally shown with the serine kinase protein kinase A (PKA). Noteworthy, dephosphorylation of the low-molecular weight substrate O-phospho-L-tyrosine (pTyr) by alkaline and acid phosphatase could also be monitored in the presence of CB7 and berberine (see Fig. 11) as a reporter pair at micromolar concentration of pTyr. 109 In another study, the activity of kinases and phosphatases was explored with cavitand 1 (Fig. 10) and DSMI (Fig. 11). 110 DSMI has a lower affinity to the cavitand (K d ¼ 23.1 mM) than the dye FAMR (K d ¼ 1.51 mM), which was used for following Fig. 28 Monitoring the activity of lysine methyltransferases with the CX4/LCG reporter pair. The assay is based on the high affinity of trimethylalkylammonium residues to the CX4 receptor, which displaces the fluorescent dye LCG in the course of the enzyme-induced methylation of the lysine side chain. Fig. 29 Monitoring the demethylation of the trimethylated lysine residue 9 of the substrate H3K9Me 3 (see text for peptide sequence) by the histone demethylase JMJD2E. The cavitand 1 binds the trimethylammonium side chain of the Lys(Me) 3 residue (blue rectangle), whereas the demethylated product H3K9 does not bind efficiently. The fluorescent dye FAMR will thus bind after demethylation leading to a fluorescence decrease as a response to the lysine demethylase activity of JMJD2E.
The tandem assay was performed with protein kinase A (PKA) and aurora B kinase, which are both known to phosphorylate serine residues. The required buffer additives ATP, cAMP, and Mg 2+ had no inuence on the cavitand 1/DSMI reporter pair and the uorescence increased as expected due to the phosphorylation of the serine residue in H3. The observed reaction rates were compared to reaction rates determined by MALDI-MS, which agreed very well. This assay was then used to explore the inuence of posttranslational modications of H3 on the PKA activity. This indicated that trimethylated H3K9Me 3 and acetylated H3K9Ac were phosphorylated at comparable rates by PKA, whereas aurora B kinase phosphorylation was much slower with the dimethylated substrate H3K9Me 2 compared to unmodied H3 in accordance with the literature. Noteworthy, the methylated lysine residues did not detrimentally interfere with detecting the phosphorylation reaction by the reporter pair as conrmed by MALDI-MS despite their stronger affinity to the cavitand 1. Finally, monitoring of dephosphorylation was shown with alkaline phosphatase and CREBtide, which was C-terminally modied with Lys and phosphorylated at the tyrosine residue, as substrate (peptide sequence: H-Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser-pTyr-Arg-Lys-OH).

Supramolecular tyrosinase assays
Another very recent supramolecular tandem enzyme assay was reported for the enzyme tyrosinase by Cai and Li (Fig. 31). 111 They explored a new reporter pair based on CB8 and thioavin-T (ThT), which forms a 2 : 2 host-dye complex with a brightly uorescent ThT excimer (l em ¼ 570 nm). 112 Tyrosinase accepts a broad variety of phenols and catechols as substrates, 113 but Tyr and DOPA did not bind to CB8. Therefore, the authors selected the tripeptide H-Tyr-Leu-Ala-OH (YLA), which had been reported to bind to CB8 with high affinity (K d ca. 0.35 mM). 114 Addition of tyrosinase into the substrate H-Tyr-Leu-Ala-H in the presence of CB8/ThT resulted in a uorescence increase suggesting that the reaction product DOPA-Leu-Ala had a lower affinity enabling reformation of the CB8/ThT 2 : 2 complex. However, it was found that DOPA-Leu-Ala had a very similar binding affinity (K d ca. 0.31 mM) as the substrate. The authors thus concluded that the uorescence increase resulted from the continued oxidation of DOPA-Leu-Ala to the respective quinones. The latter then form a complex product mixture with overall lower binding affinity than Tyr-Leu-Ala or DOPA-Leu-Ala.

Emerging applications of supramolecular enzyme assays
Within this nal section, we are going to focus on applications of tandem assays. Since their infancy ca. 15 years ago, the applicability of supramolecular tandem enzyme assays has been widely demonstrated and nowadays includes inhibition studies and screening of compound libraries (Section 6.1) as well as the detection of enzyme activity for clinical diagnosis (Section 6.2) and microbial screenings (Section 6.3). The concept of supramolecular tandem enzyme assays can also be used to afford highly selective biosensors (Section 6.4) and can be transferred to the detection of enzyme activity by magnetic resonance imaging (MRI) as an alternative read-out to optical methods (Section 6.5).

Inhibitor screening
Already during the initial report on supramolecular tandem enzyme assays, inhibition constant determinations of known inhibitors have been included to demonstrate the utility of tandem enzyme assays for enzymological investigations or for drug screening applications (see Table 1 for a current overview). This included the arginase inhibitors S-(2-boronoethyl)-Lcysteine and 2-(S)-amino-6-boronohexanoic acid, 67,115 inhibition of diamine oxidase by cyanide, 67 inhibition of thermolysin by phosphoramidon, 98 inhibition of acetylcholine and butyrylcholine esterase by tacrine and huperzine A, 76,82 and the inhibition of the lysine methyltransferase HKMT Dim-5 by 1,10phenanthroline. 104 More recently, this utility of tandem enzyme assays was also demonstrated by Hooley and Zhong for inhibition of the lysine demethylase JMJD2E by the 2-oxoglutarate analogue 2,4-carboxypyridine 105 and by Cai and Li for the tyrosinase inhibitor kojic acid. 111 Within these reports ( Table 1, entries 1-8), inhibition was quantied by IC 50 values, which is the half maximal inhibitory concentration, at which the enzymatic reaction rate is decreased by 50%. Therefore, the time-dependent uorescence changes are recorded at varying inhibitor concentrations and the initial parts are linearly tted to obtain enzymatic reaction rates in relative uorescence intensity units. This is then plotted against the inhibitor concentrations and the resulting dose-response curves are analysed with the Hill equation to afford the IC 50 values, which can be converted into inhibition constants, K i , by considering the enzyme concentration, [E]: IC 50 116 This is a suitable approach that can be widely used for substrate-selective assays or when the binding constants of the products in product-selective assays are too low to afford a quantitative displacement of the uorescent dye.
Even more desirable are, however, reporter pairs that respond linearly to the product concentration. This enables very robust assays, a straightforward quantitative evaluation, and it affords enzymatic reaction rates in molar instead of relative uorescence units. Critical to the further development of applications of supramolecular tandem enzyme assays are thus reporter pairs with improved properties. As an example, amino acid decarboxylases were initially monitored with the CB7/ dapoxyl and CX4/DBO reporter pairs. 59 The resulting assays were sufficiently robust to enable enzyme activity detection with crude cell extracts and the determination of the Michaelis-Menten constant, K m . However, the catalytic turnover number, k cat was not reported, because a kinetic analysis would have become too involved. 26,59 These assays were subsequently used by Wang et al. to identify new inhibitors of lysine decarboxylase 117,118 and arginine decarboxylase (Table 1, entries 9-11). 71 A further improved version of a supramolecular tandem enzyme assay for ornithine decarboxylase (ODC) was reported more recently by us. 60 The major improvement was achieved by using DSMI as the uorescent dye and CB6 as the macrocyclic receptor. CB6 has a higher affinity to the ODC product putrescine than CB7 or CX4 and the uorescence of DSMI is signicantly enhanced by CB6 by a factor of >100. 119 Consequently, addition of ornithine resulted in a linear time-dependent decrease in uorescence until a plateau region is reached (Fig. 32). In contrast to common uorescence progress curves in enzyme assays, the plateau does not indicate that all substrate is converted into the product, but that all CB6 is occupied by the enzymatic product putrescine. This was conrmed by addition of excess CB6, which binds to the DSMI in free solution leading to a sharp increase and a subsequent time-dependent linear decrease indicative of the continued progress of the enzymatic reaction. The uorescence units are then easily converted into product concentrations, [P], because [P] ¼ 0 before addition of enzyme and [P] ¼ [CB6] tot ¼ [DSMI] displaced at the plateau (Fig. 32). This enabled a thorough enzyme kinetic investigation yielding a catalytic turnover number of k cat ¼ (0.12 AE 0.01) s À1 and a Michaelis-Menten constant of K M ¼ (24 AE 1) mM for human ODC expressed in HEK cells. Moreover, the CB6/DSMI reporter pair enabled to determine the inhibition constants of the ODC inhibitors epigallocatechin gallate (EGCG) and a-diuoromethylornithine (DFMO). The latter was selected for a more detailed investigation on the inhibition mode (Fig. 33). At short incubation times, DFMO showed competitive inhibition in a Lineweaver-Burk plot (Fig. 33a). When the logarithmic initial reaction rates at constant DFMO concentrations were plotted against varying incubation times, the expected linear dependence was found in accordance with a suicide inhibition mode of DFMO (Fig. 33b). Similar studies to identify the mode of inhibition were subsequently performed with the reporter pair CX4/LCG and PKA (see Section 5.3), which identied Nethylmaleimide (NEM) as a suicide inhibitor of PKA (Table 1). 109 Actual screening applications are commonly performed in 96 and 384-well microtiter plates and require robust assay procedures. 60,65,90,102,108,110 The ODC assay was found to be tolerant to the required buffer additives dithiothreitol (DTT), ethylenediaminetetraacetic acid (EDTA), pyridoxal-5 0 -phosphate (PLP), Tween 80, and 1% DMSO and could be miniaturized to the microplate format to demonstrate its potential in HTS applications. The performance of the assay was excellent and gave a Z 0 factor 0.96 in 96-well microplates and of 0.90 in 384 well-microplates. Supramolecular tandem enzyme assays may thus provide an exceptional and versatile platform for rapid screening of pharmaceuticals, bioengineered enzymes, and related analytes. This was subsequently also shown by Guo and Wang, who screened a compound library with the CX4/OX1 reporter pair to identify FMO3 inhibitors (see Section 4.3 for the assay). The library contained the ingredients from a traditional Chinese medicine composed of eleven herbs with known antithrombotic activity and the screening identied several previously unknown inhibitors of FMO3 (see Table 1). 77 Moreover, the DPP4 assay by Wang and Bi (see Section 5.1) was validated with saxagliptin as a known inhibitor and then used to screen a library of >300 compounds from traditional Chinese medicine for potential inhibitory effects on DPP4. 102 This identied the polyphenols salvianolic acid C, herbacetin, and ellagic acid as previously unknown DPP4 inhibitors with micromolar IC 50 values.

Biomarker diagnostics
The activity of specic enzymes is also an important parameter in clinical diagnostics. 120 For example, pepsin activity is absent in saliva of healthy people, but found in patients suffering from gastroesophageal reux disease (GERD). Pepsin activity may be even detected before patients develop any symptoms or before any diagnostic features in medical imaging such that pepsin activity in saliva is an ideal biomarker for GERD. The pepsin assay from the Guo group (Section 5.1) based on the CX4/LCG reporter pair could detect pepsin in acidic articial saliva with an excellent limit of detection (LOD) of (329 AE 10) ng mL À1 , which enables the prompt detection of typical pepsin levels in GERD patients. 73 As another example, DPP4 activity was found to be higher in mice fed with a high fat diet (HFD) compared to normal fed mice and blood samples from type II diabetes patients had also a higher DPP4 activity than healthy individuals as controls. DPP4 activity is thus of interest in clinical diagnosis and the supramolecular tandem DPP4 assay (Section 5.1) was compared to a commercial ELISA kit for DPP4 quantication. 102 DPP4 concentrations were quantied in clinical blood plasma samples based on the linear dependence of the hydrolysis rate of the Gly-Pro-Phe-Gly substrate by DPP4 in the tandem enzyme assay. The detected DPP4 concentration correlated well with the results from the ELISA kit and a higher DPP4 activity in blood plasma samples from type II diabetes patients was clearly conrmed. This example further demonstrated that supramolecular tandem assays may become a complementary tool to established immunoassays for clinical biomarker detection.

Screening of microbial metabolic activity
In addition to detecting enzyme activity in clinical samples, supramolecular tandem enzyme assays can also be used to explore microbial metabolic pathways. This application was inspired by the discovery that CB7 and CB8 can bind a great variety of steroids with partly astonishing selectivity. 65 For example, CB8 binds the male sex hormone and anabolic steroid testosterone with nanomolar affinity, which can be detected by a ternary complex of CB8 with two encapsulated berberine (BE) molecules as a self-assembled uorescent chemosensor (see Fig. 10 and 11 for structures). 121 When the berberine molecules are displaced by testosterone, uorescence decreases, because berberine is nearly nonuorescent in water. 65,122 There is an increasing interest to identify steroid-degrading bacteria for wastewater treatment, which suggested to use a supramolecular tandem assay for monitoring the depletion of testosterone by steroid-degrading bacteria (Fig. 34). 122 Therefore, two bacterial strains were selected, Buttiauxella sp. S19-1 and Marinobacter adhaerens HP15, which are known to degrade testosterone and have no steroid-degrading capability, respectively. The experimental design involved positive and negative controls and clearly identied the testosterone-  Monitoring the degradation of steroids by steroid-depleting bacteria. The steroids bind sufficiently strong to CB8 to displace the dye BE. The degradation products have a lower affinity and the CB8/ BE 2 complex can re-form as indicated by an increased intensity. The assay was conducted directly in aliquots from the bacterial culture broths.
degrading capability of Buttiauxella sp. S19-1. The degradation was followed by a time-dependent increase in uorescence intensity, because the degradation of the strong binder testosterone allowed the reformation of the uorescent CB8/BE 2 complex. Noteworthy, the assay was suitable to follow the steroid degradation down to submicromolar concentrations of testosterone in the bacterial culture media with excellent condence intervals as assessed by a Z 0 factor in the range of 0.52 to 0.74.

Optical biosensor arrays based on tandem assays
In the applications summarized in the preceding sections, the supramolecular sensor was applied to detect variations in the activity of enzymes, e.g. by inhibition, in conjunction with a disease, or of different microorganisms. Alternatively, a combination of enzyme and a supramolecular sensor may afford a biosensor for the detection of the enzyme substrate as an analyte. Classical biosensors comprise an enzyme as a specic signal generator and a general transducer that converts the enzymatic reaction into a readable output signal (Fig. 35). The most established biosensors are based on electrochemical transducers coupled to oxidoreductase enzymes such as in blood glucose sensors for diabetes patients, whereas optical signal transducers have been also applied. 123,124 The comparably low selectivity and cross-reactivity of supramolecular host-guest recognition appears ideal to apply them as signal transducers for optical sensing. One possibility to take advantage of the low selectivity and cross-reactivity is the construction of differential sensor arrays, in which several supramolecular sensors react differently towards a certain analyte to generate a pattern of sensor responses. 65,[125][126][127] Multivariate data analysis then allows to relate the response pattern to the presence of a certain analyte. This is reminiscent of the sensation of smell, in which a response pattern from hundreds of olfactory receptors is assigned by the brain to sensation of a certain scent. Another possibility arising from the comparably low selectivity of host-guest recognition is their use as optical signal transducers for a variety of related enzymes. The latter than function as the a specic signal generator due to the high selectivity of the enzymes.
Highly selective sensing of various amino acids has been demonstrated with amino acid decarboxylases and the CB7/ dapoxyl reporter pair as a signal transducer. 90 Four sensors were included for the detection of lysine, arginine, tyrosine, and histidine with their respective decarboxylases. The detection limit of all four amino acids was in the low micromolar range with no further optimization and no cross-reactivity between the different amino acids was noted. Moreover, the stereo-specicity of the amino acid decarboxylases was exploited to assess the enantiomeric excess (ee) of D-and L-amino acids by the biosensors, which enabled the detection of the enantiomeric excess of D-amino acids up to an ee of 99.98%.
Biosensors constructed from enzymes coupled with supramolecular sensors may also be very useful for the detection of analytes in complex matrices such as body uids or food samples. The reason is that any competing analytes in the complex matrix will afford a static and immediate response, which is constant throughout the measurement, which can be easily discriminated from the time-dependent change arising from the conversion of the analyte by the enzyme. Such endeavours are, to the best of our knowledge, so far unexplored with supramolecular tandem assays. 113,123,128 6.5 Supramolecular MRI biosensing of enzyme activity In vivo imaging of enzyme activity, either by optical probes or by magnetic resonance imaging (MRI) as an imaging modality, is another application area, in which supramolecular tandem enzyme assays could make an impact. Compared to passive targeting probes, enzyme-activatable imaging probes are usually most desirable, because they offer an improved signalto-background ratio and, thus, contrast enhancement. 129,130 One possibility to transfer supramolecular tandem enzyme assays to imaging is 129 Xe MRI. Using hyperpolarized 129 Xe in conjunction with chemical exchange saturation transfer (hyper-CEST) affords highly sensitive detection and has the potential to become a clinical molecular imaging modality. [131][132][133][134] Hyper-CEST requires a sensing unit that binds Xe leading a complexation-induced shi of the 129 Xe NMR peak and sufficiently rapid exchange with a pool of free Xe atoms. Since numerous supramolecular host molecules including cyclodextrins, 135,136 hemicarcerands, 137,138 calixarenes, 139-142 cryptophanes, [143][144][145][146][147][148][149][150][151] cucurbiturils, [152][153][154][155][156][157][158][159] and cavitands 160,161 are known to bind Xe, competitive occupation of the cavity by a substrate or product of an enzymatic reaction could modulate the Xe exchange, which becomes detectable by hyper-CEST.
Among the available Xe-binding host molecules, cryptophanes and cucurbiturils appear most promising, 131,132,134,154,155 whereas the cavity of the former is too small to allow competitive complexation by larger guests. The application of supramolecular tandem enzyme assay in MRI with cucurbiturils follows a similar scheme as for optical detection (Fig. 36). 162,163 When the host cavity is occupied by either the substrate or the product of the enzymatic reaction guest exchange with Xe cannot proceed leading to a decrease of the hyper-CEST signal. This was shown with lysine decarboxylase (LDC), which transforms weakly binding lysine into strongly binding cadaverine and CB6 and CB7 as potential contrast agents. 162 With CB6, the Hyper-CEST spectra showed a resonance at d ¼ 193 ppm Fig. 35 Principle of a biosensor. Typically a highly selective enzymatic reaction (e.g. a oxidoreductase) is used as a specific signal generator that is coupled with a signal transducer (e.g. a electrochemical readout) to afford a readable signal in presence of the analyte. In the supramolecular variant, the specific signal generator is also an enzyme, whereas the transducer is a host-dye reporter pair affording an optical read-out signal.
attributed Xe dissolved in the buffer and a resonance at d ¼ 105 ppm assigned to Xe bound to CB6. During the progress of the enzymatic reaction, the signal at 105 ppm disappeared due to occupation of the CB6 cavity by the reaction product cadaverine, which prevented CEST of Xe with CB6. When the method was transferred from buffer solution to cell lysates, no distinct resonance was observed at d ¼ 105 ppm, but the CEST response at d ¼ 193 ppm was considerably broadened. This was assigned to additional interactions of CB6 with other competitive guest in the cell lysate. The broadened signal, however, suggested to explore hyperpolarized 129 Xe magnetization transfer with CB7 as an alternative MRI detection method, which gave an excellent contrast with phantom samples in MRI. In a subsequent extension of this work, 163 time-resolved monitoring was achieved, which afforded initial reaction rates and product concentrations.

Conclusions
In this review, we have comprehensively summarized the current state-of-the-art of enzyme assays based on supramolecular chemosensors. Chemosensors that can discriminate enzymatic substrate and product provide a unique and innovative approach to monitor enzyme activity that is label-free, provides high sensitivity at low cost, and is compatible with HTS applications. We have summarized the design tactics for supramolecular tandem enzyme assays, that have been utilized to develop substrate-selective and product-selective tandem assays for various enzymatic reactions. This includes the initial use of low-molecular weight substrates and products that are nearly completely immersed in the cavity of the chemosensors, as well as more recent extensions to peptides that are hydrolysed or functionalized at specic amino acid side chain residues by oxidation, phosphorylation and dephosphorylation, or methylation and demethylation by different enzymes. With improved host-dye reporter pairs, the robustness of the assays was also improved leading to the rst demonstration of advanced applications of tandem assays in inhibitor screening, biomarker detection, optical biosensors arrays, and MRI biosensing.

Conflicts of interest
There are no conicts to declare.