Structure–function relationships in aryl diazirines reveal optimal design features to maximize C–H insertion

Diazirine reagents allow for the ready generation of carbenes upon photochemical, thermal, or electrical stimulation. Because carbenes formed in this way can undergo rapid insertion into any nearby C–H, O–H or N–H bond, molecules that encode diazirine functions have emerged as privileged tools in applications ranging from biological target identification and proteomics through to polymer crosslinking and adhesion. Here we use a combination of experimental and computational methods to complete the first comprehensive survey of diazirine structure–function relationships, with a particular focus on thermal activation methods. We reveal a striking ability to vary the activation energy and activation temperature of aryl diazirines through the rational manipulation of electronic properties. Significantly, we show that electron-rich diazirines have greatly enhanced efficacy toward C–H insertion, under both thermal and photochemical activation conditions. We expect these results to lead to significant improvements in diazirine-based chemical probes and polymer crosslinkers.


Introduction
The insertion of high-energy carbene or nitrene species into unactivated C-H, O-H and N-H bonds constitutes a powerful strategy for forming new bonds in complex biological, chemical, or mechanical mixtures. 1,2 The ability to rapidly and controllably generate carbenes from stable and readily accessed precursor molecules allows one to label (or 'tag') binding partners, 3 isolate reaction products, 4 or crosslink polymer materials. [5][6][7] In recent years, diazirine motifs have enjoyed enormous popularity as carbene precursors, for applications ranging from biological target identication through to adhesion of commodity plastics (Fig. 1). Part of this popularity can be attributed to their small size and ready synthetic access. 8,9 Of course, for any reactive reporter group the most important criteria are that the reporter be stable under any required synthesis, handling, and experimental conditions, yet be easy to activate (ideally with good control over spatial localization) when needed. Diazirines excel when measured against these criteria as well; most diazirines are stable to commonly used synthetic reaction conditions (including strong Brønsted 5 and Lewis 10,11 acids), and many diazirine-containing molecules are thermally stable. When desired, diazirines can be readily activated using light (ca. 350-365 nm), 8 heat (typically 110-130 C), 5,12 resonance energy transfer 13 or electrical potential, 14 resulting in the expulsion of nitrogen gas and the formation of the corresponding carbene. The long wavelength of light required for photochemical activation (outside the window where biological and polymer materials absorb) and the low temperature required for thermal activation (below the melting temperature of many commodity polymers) are critical factors that have contributed to the broad uptake of diazirines by research groups around the world.
The substituents on either side of the CN 2 core are known to inuence the properties of both the parent diazirine molecule and the product carbene. For example, dialkyl diazirines generally give rise to ground-state singlet carbenes, due to hyperconjugative donation of lled C-H orbitals into the unoccupied p orbital of the singlet carbene group. 15,16 Carbenes generated from phenyl diazirines (or from the isomeric linear diazo compound) can exist in either singlet or triplet forms; the triplet is generally found to be the ground state for these species, 17 but in many cases the singlet carbene is believed to be produced initially, 18,19 aer which intersystem crossing (ISC) facilitates relaxation to the observed triplet (Fig. 1b). 17 The energy gap between the singlet and triplet states is crucially important, since the two types of carbenes undergo different types of reactions with X-H bonds. Triplet reactivity is dominated by stepwise hydrogen abstraction/radical recombination pathways that can lead to undesirable side reactions, while singlet X-H insertion is believed to proceed by a concerted-but two-phase-pathway wherein the empty carbene p orbital rst receives electron donation from the X-H s bonding orbital, and then the lone pair on the carbene backdonates into the X-H s* orbital. 16 Further complicating matters, triplet carbenes are known to undergo rapid reactions with adventitious O 2 (ultimately leading to the production of ketone byproducts) while singlet carbenes appear to be immune to this undesirable reaction pathway. 20 As is the case with nitrene species, diazirines and carbenes can undergo unwanted rearrangement reactions; Brunner showed in 1980 that the installation of an a-triuoromethyl group reduced the likelihood of such rearrangements, leading to improved overall stability and more controllable reactivity. 8 Since Brunner's seminal report, the triuoromethyl phenyl diazirine group (TPD; Fig. 1d) has become the most widely employed carbene precursor in the chemical literature. Tri-uoromethyl aryl diazirines are now used in a host of biological target identication experiments (Fig. 1c) 3,21-25 where they are found to outperform alternative labeling groups like nitrenes or benzophenones, 26,27 and the TPD motif has been genetically encoded into protein structures using expanded genome techniques. 28 TPD has also been used to map the localized protein environment on T cells using an innovative m-mapping technique facilitated by Dexter energy transfer, 13 and tri-uoromethyl aryl diazirines have been designed into bioadhesives to expedite wound closure. 14 In polymer science, tri-uoromethyl aryl diazirines have been conjugated to uorophores and photosensitizers in order to imbue nylon 29 and spunbond polypropylene 30 materials with new functionality. Silicon and silicon nitride surfaces can be similarly functionalized. 31 TPD groups have also been used on surfaces to capture small molecule reaction products 4 and drug molecules, 32 and have been incorporated into polymer crosslinkers that can be used to strengthen woven polyethylene fabric 5 or provide adhesion between polypropylene or polyethylene surfaces (Fig. 1e). 12 These latter applications using commodity aliphatic polymers provide a particularly stringent test of a diazirine's ability to support C-H insertion, since (to a rst approximation, at least) such polymers only contain C-C and C-H bonds, and therefore lack anything that would be traditionally viewed as a reactive functional group.
Given the extensive utility of TPD groups, it is surprising that linear free energy relationships in triuoromethyl aryl diazirines have received very little attention. (By contrast, Hammett relationships in a-chloro diazirines have been extensively studied, but these species are known to participate in a variety of reactions that are not available to the much more important a-triuoromethyl congeners. 33 ) As a result, incorporation of a triuoromethyl aryl diazirine group into a molecule of interest (whether for biological or materials science applications) has typically been done in an ad hoc manner, without any consideration of how the electronics of the aryl diazirine group will inuence the generation and reactivity of the resulting carbene. While a variety of electron-rich, electron-poor, and electronneutral triuoromethyl aryl diazirines have been described in the literature, only in very rare cases has the inuence of electron-donating or electron-withdrawing groups on reaction outcome (especially under thermal activation conditions) been discussed, and the conclusions from these reports are somewhat contradictory.
In 2011, Song and Sheridan reported a study of metamethoxy vs. para-methoxyphenyl triuoromethyl diazirine, and showed that the para-methoxy substituent was capable of supporting a singlet carbene ground state (at 7 K in a frozen nitrogen matrix) due to resonance stabilization. 20 Two years later, Raimer and Lindel studied this same para-methoxy tri-uoromethyl diazirine molecule, and found that activation in the presence of phenols (including tyrosine derivatives) led to production of the desired singlet carbene, which could then undergo formal O-H and C-H insertions with the substrate. 34 Interestingly, however, these reactions were shown in all cases to be the result of initial protonation of the singlet carbene to generate a benzylic cation, which could then either be quenched by reaction with the phenol (or phenoxide anion) or else undergo Friedel-Cras reaction with the electron-rich aromatic ring. 34 Various lines of evidence were used to rule out the existence of any direct C-H insertion reactions. While the Sheridan and Lindel papers provide important precedent in favour of the hypothesis that electron-rich triuoromethyl aryl diazirines might provide an improved reactivity prole relative to electronpoor or electron-neutral analogues, neither report demonstrated direct C-H insertion reactions with aliphatic substrates. As a result, it remains an open question whether electron-rich aryl diazirines would be preferred or not, in the applications described above.
To further complicate matters, a recent report by Kumar, Tipton and Manetsch provided compelling evidence that electron-poor diazirines are superior, due to improved stability under ambient light, and that this lack of electron density does not impair the molecules' ability to undergo C-H insertion. 35 Meanwhile, Kanaoka and co-workers have advocated for the use of diazirines bearing both an electron-donating alkoxy group and an electron-withdrawing nitro substituent, due to improved optical properties. 36 While the Kanaoka group showed the performance of their molecules in reacting with cyclohexane (an important benchmarking experiment for diazirine reactivity) they did not compare the efficacy of their structures to that of the parent TPD molecule.
These sparse reports leave the experimentalist who might want to employ a diazirine for a particular application in a quandary. Should an electron-rich or electron-poor tri-uoromethyl aryl diazirine be used if one wants to maximize the potential for C-H insertion under standard laboratory conditions? If an electron-rich system is preferred in order to stabilize a singlet carbene intermediate, how electron rich does it need to be? Given that the optimized electronic properties will likely increase the synthetic challenge in preparing the molecule, is the improved performance sufficient to justify the increased synthetic effort and/or increased cost of reagents?
The situation becomes even more complex when activation temperature is taken into account. The few structure-function studies on triuoromethyl aryl diazirines alluded to above all focused on photochemical activation, since this is the dominant excitation method used for biological studies. For materials science work, however, thermal activation is generally preferred; 5,12 while commodity polymers like polyethylene and polypropylene do not absorb light in the window used for photochemical diazirine activation, they do very efficiently scatter light and are therefore visibly opaque or white solidsmaking the use of photochemical activation techniques a substantial challenge for real-world samples. An understanding of how activation temperature changes with diazirine electronics is therefore critically important to the design of new polymer crosslinkers and adhesives. 37 Moreover, the thermal activation temperature dictates the explosivity (or lack thereof) for the diazirine molecule being used: 5,38 an understanding of how activation temperature can be tuned is therefore necessary for designing safe and effective diazirine-based reagents for all manner of applications.
In this work (Fig. 1f) we use a combination of experimental and computational methods to conduct an extensive structurefunction study of the properties and reactivity of aryl diazirines-especially those bearing an a-triuoromethyl substituent. We particularly focus our experimental work on the measurement of activation temperatures by differential scanning calorimetry (DSC), and also explicitly compare the ability of electron-rich, electron-neutral, and electron-poor molecules to engage in C-H insertion reactions under both thermal and photochemical activation conditions. Ultimately we anticipate that the conclusions from this work will inform the design of molecular probes, small-molecule capture agents, and polymer crosslinkers with improved efficacy.

Tunability of optical absorbance characteristics
We synthesized (or, in a few cases, purchased) several representative triuoromethyl aryl diazirines, incorporating electronrich, electron-neutral, and electron-withdrawing groups on the aromatic ring (see Table 1 for substituents). Table 1 Effect of aryl substituent electronics on activation temperature and on activation free energy Characterization of archetypal electron-rich (4-OMe-C 6 H 4 , 4-OPh-C 6 H 4 ), electron-neutral (C 6 H 5 , 4-t-Bu-C 6 H 4 ), and electronpoor (3-OMe-C 6 H 4 , 3,5-(OMe) 2 -C 6 H 3 , 4-NO 2 -C 6 H 4 ) tri-uoromethyl aryl diazirines by UV/Vis spectroscopy revealed interesting trends. As shown in Fig. 2, electron-rich and highly electron-poor diazirines both showed strong absorbances in the diazirine activation window of ca. 320-410 nm. However, electron-neutral and moderately electron-poor triuoromethyl aryl diazirines had attenuated absorbances in this range. It would seem, therefore, that strongly electron-rich or electronpoor diazirines might be preferred for photochemical activation, simply on the basis of their improved extinction coefficients. This is an important observation, given that the vast majority of biological target identication studies in which diazirines have been used have featured electron-neutral substrates.
Of equal importance, we observed a >30 nm shi to higher wavelengths when moving from electron-poor to electron-rich triuoromethyl aryl diazirines. The ability to shi the diazirine activation bands to longer wavelength is useful both in terms of evading background absorbance and allowing the use of cheaper and more readily purchased light sources.

Tunability of activation temperature and activation free energy
Each of the triuoromethyl aryl diazirine molecules was analyzed by DSC (refer to the ESI † for representative traces) to measure the onset temperature for diazirine activation (T onset ) 39 and the peak temperature (T peak ; the temperature at which the diazirine activation exotherm reaches maximal heat ow). At the same time, we computationally determined the activation free energy (DG ‡ ) for the diazirine activation reaction.
The data (Table 1) revealed a difference of ca. 30 C in thermal activation temperatures, as we moved from electronrich triuoromethyl aryl diazirines (e.g. R ¼ 4-OCH 3 ; T onset ¼ 88 C; T peak ¼ 113 C) to electron-poor analogues (e.g. R ¼ 4-NO 2 ; T onset ¼ 118 C; T peak ¼ 144 C). This substantial difference in activation temperature corresponded to an equally signicant difference in calculated activation free energy (DDG ‡ > 12 kJ mol À1 ). Indeed, a strong positive correlation between all of the experimentally determined T onset and T peak values and the corresponding calculated activation free energies was observed (Fig. 3). This agreement between theory and experiment, con-rming that T onset is a reection of the reaction barrier, is a necessary prerequisite for using computational methods to interrogate features of the reaction that cannot be easily observed experimentally.
The 30 C span in activation temperatures is important from the perspective of experimental planning. For some applications where increased thermal stability is required (e.g. application of diazirine crosslinkers to polymer melts, or certain biological experiments where size limitations on a molecule may preclude the addition of molecular weight necessary to mitigate the explosivity risk that would otherwise accompany a low activation temperature) electron-poor diazirines with high activation temperatures will be preferred. For many applications, however (especially for polymer adhesion applications where one does not wish to melt the underlying polymer substrate), lower activation temperatures are highly desirable. In this context, the observation that electron-rich tri-uoromethyl aryl diazirines can be activated at <90 C is particularly relevant to the development of improved reagents for materials science.

Quantication of linear free-energy relationships
The observed variation in activation temperatures can also shed light onto the mechanism of diazirine activation. To explore this, we plotted the data from Table 1 against various empirically derived Hammett s values available from prior studies of reactions that are known to occur by polar or radical mechanisms. These included: (1) Hammett's original s parameters derived from the study of para-substituted benzoic acid ionization; 40 (2)    cyclodimerization of triuorostyrenes. 44 In comparisons of this type, a strong positive correlation to s + is generally taken as evidence in favour of some degree of carbocation character in the transition state, while correlation with any of the various sc parameters is taken as evidence of radical character. 45 Experimental T onset and T peak data were found to give robust linear ts (R 2 $ 0.95) to empirically derived s + p values (Fig. 4). Calculated DG ‡ values likewise showed a strong correlation to s + p (R 2 ¼ 0.91). By contrast, very poor ts (R 2 < 0.1) were observed to empirically derived sc values from Creary or Jiang (see Fig. S2 in the ESI † for plots of T onset and T peak vs. all s parameters). Together, these results argue in favour of an emerging vacant orbital at the benzylic centre during the transition state, and against the presence of any signicant spin density at this position. These data provide compelling additional experimental support for the hypothesis that triuoromethyl aryl diazirines initially form singlet carbenes upon activation, and that triplet carbenes are formed via a subsequent relaxation step.
As discussed above, triuoromethyl aryl diazirines have dominated diazirine research ever since Brunner's publication in 1980. Perhaps because of this dominance, there are few direct comparisons of a-triuoromethyl diazirines to diazirines containing different a-substituents. In an effort to partially address this deciency, we surveyed ve other a-substituents and compared their T onset and T peak values ( Table 2).
Lower activation temperatures were observed for a-H, achloro and a-methyl diazirines relative to a-CF 3 , while a representative a-uoro diazirine had slightly higher T onset and T peak temperatures. The only a-methoxy compound studied was too unstable to properly study experimentally, but appeared to have a very low decomposition temperature. 46 Preliminary multi-reference calculations indicate that there are varying degrees of multi-reference character in the transition states of the alphasubstituted species. The poor treatment of multi-reference systems by M06-2X leads to a weaker correlation between the calculated barrier heights and T onset for the data in Table 2 than was seen for the equivalent data in Table 1.

Expanded in silico Hammett studies
The robust linear free energy relationship observed in Fig. 4 stimulated several additional questions: 1. Would aryl diazirines with alpha substituents other than CF 3 reveal similar tunability in activation temperature through the addition of electron donating or electron withdrawing groups on the aromatic ring? We were particularly interested here to study molecules with p donor groups at the alpha position (e.g. OMe or F). By donating into the unlled p orbital of the singlet carbene, such groups could in principle alter the electronic preferences of the carbene intermediate, as well as the transition state energies.
2. Is it possible through judicious tuning of the electronics of an aryl diazirine to stabilize the singlet carbene sufficiently well that it becomes the lower energy state for the molecule? As discussed above, data from Sheridan and Lindel strongly implicate the involvement of singlet carbenes following activation of triuoromethyl 4-methoxyphenyl diazirine, but it was not clear how this molecule ts into a larger trend. 47 3. If a singlet state were favoured, would this improve the ability of the molecule to undergo C-H insertion with aliphatic substrates? Or would the stabilization that is necessary to lower   )) associated with conversion of the diazirine into the corresponding carbene in vacuum. e The a-methoxy diazirine was too unstable to be analyzed by DSC, which is consistent with the low DG ‡ that was found computationally, and with results from a different group. 46 the energy of the singlet state below that of the triplet necessarily then raise the activation barrier for C-H insertion, such that this becomes a less efficient process? 4. Given the very low activation barrier for a-methoxy diazirines, would these species be better or worse reagents from the perspective of C-H insertion? Alkoxy diazirines are valuable intermediates in glycoside synthesis, 48 but less is known about how their properties compare to the more commonly exploited triuoromethyl diazirines. 46,49 Some of these questions are challenging to study experimentally. For example, the difficulty of working with a-methoxy diazirines (as noted above) makes it a challenge to derive analytically precise data describing their activation. Similarly, exploring the farther reaches of the Hammett plot by studying the inclusion of stronger electron donating groups like NMe 2 (s + p ¼ -1.7) or NH 2 (s + p ¼ -1. 3) was ruled out as potentially hazardous to laboratory personnel, since these molecules would have signicantly lower activation temperatures. Even studying the difference in performance for a p-OH group (s + p ¼ -0.92) relative to the p-OMe substituent (s + p ¼ -0.78) was ruled out experimentally, due to concerns that hydrogen bonding for the phenol in the solid state might complicate our analysis. Moreover, Hatanaka and co-workers have previously reported that this phenol is unstable and difficult to isolate. 10 Of course, none of the above concerns apply to in silico experiments. We therefore sought to explore the above questions computationally, using an expanded Hammett series (R ¼ 4-NMe 2 , 4-NH 2 , 4-OH, 4-OMe, 4-t-Bu, 4-CH 3 , 4-H, 4-Cl, 4-CF 3 , 4-CN and 4-NO 2 ) and four different alpha substituents (X ¼ CF 3 , Cl, F, and OCH 3 ).
All 44 diazirine activation reactions were modelled using M06-2X-D3/6-31G(d,p) methods, and the calculated activation free energies (DG ‡ ) are shown in Fig. 5 (see Table S3 † for complete tabulated data). The series of a-triuoromethyl diazirines gave the largest positive slope of any of the four families of substrates. Because we know from Fig. 3 and 4 that diazirine activation energy tightly corresponds to T onset and T peak temperatures, this means that activation temperature would be much less tunable for a-uoro, a-chloro and a-methoxy diazirines. In fact, the slope is essentially zero across the Hammett series when X ¼ F (see Table S3 † for tabulated slopes), and becomes negative when X ¼ OCH 3 .
A series of computed isodesmic reactions (Fig. S3 †) suggested that the trends observed in Fig. 5 are principally due to (de)stabilization of the transition states, rather than alteration of the ground-state diazirine energies. These data indicate that when X ¼ CF 3 , electron-donating groups located across the aromatic ring from the diazirine centre can help to stabilize the empty p orbital that is evolving within the transition state. Once again this is consistent with a transition structure that is singlet-like, rather than triplet-like, and provides additional data in support of the hypothesis that singlet carbenes are initially formed following diazirine activation. This effect is blunted by incorporation of groups with increasing p-donating ability (X ¼ Cl < F < OCH 3 ) since these groups can themselves donate electron density to help stabilize the evolving empty p orbital. By the time we reach the strongest p-donor (X ¼ OCH 3 ), the evolving empty p orbital is fully stabilized, and we therefore observe no change in transition-state stabilization with differing substituents on the aromatic ring.

Assessment of singlet-triplet gaps
We next calculated the singlet-triplet energy gaps for our four Hammett series using domain based local pair natural orbital (DLPNO) coupled cluster methods ( Fig. 6; see Table S6 † for comparative data using different levels of theory). 50 For each of the p-donors included in our study (X ¼ Cl, F, or OCH 3 ), the singlet was always the lowest energy state for the carbene, regardless of the identity of the para substituent (R) on the aromatic ring. As we saw with transition state energies, the ability of the a-substituent to stabilize the singlet carbene through the donation of electron density into the empty p orbital evidently outweighs other effects due to resonance donation by the distal R group.
For the a-triuoromethyl series, we saw more interesting behaviour, wherein the singlet energy was dramatically affected by the ability of the R group to donate electron density, while the triplet energy remained essentially constant across the Hammett series. The result is a switch in the energy ordering of the two possible carbenes-when the aryl group was substituted with electron-neutral or electron-poor groups at the para-position, the triplet carbene was the lower energy species, but when a strong electron-donating group was added to the para-position of the aromatic ring, the singlet carbene became lower in energy. When R ¼ OCH 3 , the singlet was calculated to be 6.9 kJ mol À1 lower in free energy (DLPNO-CCSD(T)/cc-pV(T-Q) Z//M06-2X-D3/6-31G(d,p)).
The data in Fig. 6 indicate two different options for producing aryl carbenes in which the singlet state is predominant: one may either employ an electron-donating a substituent such as an alkoxy group (in which case the electronics of the aromatic ring are largely immaterial) or else one can maintain the a-CF 3 group that is widely used in literature crosslinking studies but add a sufficiently electron-donating para substituent to the aromatic ring.

Calculation of C-H insertion barriers
Applications of diazirine groups in chemical biology or materials science demand that the carbene generated in situ be able to undergo efficient C-H insertion (or occasionally O-H insertion). However, the percent conversion for this process is typically quite poor (oen <10%) because of the occurrence of side reactions. One particularly troublesome side-reaction for carbenes-oen accounting for >50% of the mass balance in model studies of C-H insertion reactions-is quenching by adventitious oxygen to produce undesired ketone side products. It is thought that this mainly occurs through reaction of 3 O 2 with the triplet carbene. As such, the use of stabilized singlet carbenes (wherein little if any triplet is ever formed) may provide improved C-H insertion yields. On the other hand, if the singlet carbenes are too stabilized, then a higher energy barrier will be required for C-H insertion.
Mindful of the potential benets of improved C-H insertion reactions elicited by an ability to favour a singlet carbene intermediate, we computed the energy barriers for aliphatic C-H insertion (using the central CH 2 motif of propane as a model of a typical aliphatic group), for all 88 singlet and triplet carbenes derived from aryl diazirines where X ¼ CF 3 , Cl, F, or OCH 3 , and R ¼ 4-NMe 2 , 4-NH 2 , 4-OH, 4-OMe, 4-t-Bu, 4-CH 3 , 4-H,4-Cl, 4-CF 3 , 4-CN or 4-NO 2 . Complete numerical data are tabulated in Table S3, † and are summarized graphically in Fig. 7.
The data reveal that for all four series of carbenes, the triplet C-H insertion barriers are always lower in energy than the corresponding singlet barriers, although the numbers are relatively close for electron-poor triuoromethyl aryl diazirines (D[DG] ¼ 5.5 kJ mol À1 when R ¼ NO 2 ). The lowest singlet C-H insertion barriers are found for the triuoromethyl aryl diazirine series. Critically, these barriers are all lower than the DG ‡ for the initial production of carbenes from the diazirine parent compounds (ca. 140-155 kJ mol; see Table 1 and Fig. 5).
Therefore, although the singlet C-H insertion barriers shown in Fig. 7 are higher than the barriers for the corresponding triplets, they must still be energetically achievable if the system had enough energy for diazirine activation. In other words, if a singlet a-CF 3 carbene is produced, it should be able to undergo insertion with nearby C-H bonds; the ratedetermining step for the process will be loss of N 2 from the diazirine, and the subsequent insertion will be kinetically invisible.  This is not the case for the other carbene series. For a-OCH 3 carbenes in particular, the singlet C-H insertion barriers are very high in free energy, which removes any possible benet associated with the facile diazirine activation for these molecules.
Optimal C-H insertion efficiency from an electron-rich diazirine Taken together, the above data suggest that triuoromethyl aryl diazirines bearing an electron-donating substituent at the paraposition of the aromatic ring may be optimal: they will require a lower temperature for thermal activation, they should favour a singlet ground state for the carbene intermediate, and that singlet carbene should have sufficient energy to undergo C-H insertion. But what would be the most appropriate electrondonating group to use, when seeking to balance a desirable reactivity prole with good handling characteristics? Extrapolation of the red line in Fig. 4 and estimation of initiation temperature from T onset (ref. 39) suggests that p-amino tri-uoromethyl phenyl diazirines would be unstable above ca. 40 C. In addition to obvious concerns around shock sensitivity or explosive propagation 5,38 simply making and handling such a molecule on large scale could be problematic.
We therefore identied a p-alkoxy group as the optimal substituent to attach to the triuoromethyl aryl diazirine scaffold. Our calculations suggest that it is sufficiently electron donating to favour the singlet ground state in the carbene (i.e. it falls just to the le of the intersection point in the top panel of Fig. 6), and yet our experimental measurements indicate that it is not so electron-donating as to result in an impractically low activation temperature. As an added benet, p-alkoxy tri-uoromethyl aryl diazines will have a longer wavelength l max and increased extinction coefficient associated with the diazirine activation band in the UV/Vis spectrum (Fig. 2).
Notwithstanding occasional earlier reports on the use of palkoxy triuoromethyl aryl diazirines by Sheridan, 20 Lindel, 34 Kanaoka 36 and Hashimoto, 51 direct comparisons of efficacy between electron-rich, electron-neutral, and electron-poor aryl diazirines in their ability to insert into challenging (i.e. high energy) C-H bonds are lacking in the literature. Moreover, the few literature examples where electron-rich triuoromethyl aryl diazirines are used are limited to photochemical activation methods. These examples are therefore relevant to chemical biology applications (where diazirines are almost always excited photochemically or else through energy transfer) but are less relevant to materials science applications, where thermal activation tends to predominate.
We therefore conducted a series of thermally and photochemically promoted C-H insertions with representative electron-rich, electron-neutral, and electron-poor tri-uoromethyl aryl diazirines, employing cyclohexane as a substrate (C-H bond dissociation energy ¼ 416 kJ mol À1 ). 52 Consistent with our prediction from Fig. 6 that electrondonating groups at the para-position of the aromatic ring would stabilize a singlet carbene and allow for more selective C-H insertion reactions, we found that the installation of 4-OPh or 4-OCH 3 substituents dramatically improved the isolated yield of the desired product (Table 3). This was particularly true for thermal activation conditions, in which the best-performing diazirine substrate was >10-fold more efficacious toward C-H insertion than the worst substrate. Regardless of the activation method, the 4-OCH 3 group was found to be superior to the 4-OPh group, consistent with the improved electron-donating ability of the former substituent.
Careful examination of the crude NMR spectra for each of the reactions in Table 3 (see Fig. S70 and S71 †) indicated the presence of signicant quantities of ketone side products for the less-successful reactions, consistent with the hypothesis that for electron-poor or electron-neutral triuoromethyl aryl carbenes, the triplet is the ground-state species. As further evidence for the mechanism of ketone formation, we examined the addition of triplet triuoromethyl aryl carbenes to O 2 computationally, and found this process to be essentially barrierless. Other side products appear to result from self-insertion pathways where one carbene molecule undergoes undesirable C-H insertion reactions with the aromatic moiety of the starting material. Whereas the data in Table 3 were collected using dry, degassed solvent, we also sought to study the reaction of the optimized substrate in less rigorous reaction conditions that might better mimic "real world" circumstances where diazirines need to be used. Thermal and photochemical reactions of p-methoxy triuoromethyl phenyl diazirine were therefore repeated using 9 : 1 cyclohexane : water, and each reaction was run under air instead of inert atmosphere (Scheme 1). Remarkably, the desired C-H insertion adduct was once again the only isolable product, and could be obtained in $90% yield. Indeed, the crude NMR spectra for these "wet" reactions were identical to those obtained when the reaction was run using dry, deoxygenated cyclohexane (Fig. S72 †).
While C-H insertions are oen desired, O-H insertions can be equally useful for both protein labeling 34,53 and polymer crosslinking. 5,12 Raimer and Lindel had previously shown that pmethoxy triuoromethyl phenyl diazirine could react with phenols, 34 but because the proposed mechanism involved initial protonation by the acidic phenol, it was unclear what the efficiency of O-H insertion would be with alcohols. We therefore reacted our electronically optimized diazirine with tertbutanol under both photochemical and thermal activation conditions, once again intentionally carrying out the reaction under air and using non-anhydrous solvent (Scheme 1). Satisfyingly, the total yield of O-H insertion products was approximately 90% in both cases.

Conclusions
Aryl diazirine reagents already nd broad application in biological target identication, small molecule capture, and protein-and polymer-crosslinking, as well as in experimental adhesives for both commodity polymers and living tissue. 54 However, in the vast majority of cases, the structure of the diazirine unit within these reagents has been chosen based on synthetic expedience rather than a consideration of what electronic properties might be optimal. In other words, all aryl diazirines have been assumed to be more or less equivalent in their ability to function as reactive "warheads". In contrast to this view, the data presented in the current work provide compelling evidence that the properties of aryl diazirines can be readily adjusted by tuning the electronics of the aryl substituent. In particular, the observation that simply adding an alkoxy group to an aryl diazirine can improve the C-H insertion efficiency by >10-fold relative to an electronically unoptimized diazirine should stimulate the development of ever more effective reagents for the diverse array of applications discussed above.

Data availability
Complete experimental and computational data are available in the ESI. †

Conflicts of interest
There are no conicts to declare.