Itaconate is a covalent inhibitor of the Mycobacterium tuberculosis isocitrate lyase†
Brooke X. C.
Kwai, 
a
Annabelle J.
Collins,
a
Martin J.
Middleditch,
bc
Jonathan
Sperry,
*a
Ghader
Bashiri
*bd
and
Ivanhoe K. H.
Leung
*ad
a
Annabelle J.
Collins,
a
Martin J.
Middleditch,
bc
Jonathan
Sperry,
*a
Ghader
Bashiri
*bd
and
Ivanhoe K. H.
Leung
*ad
Abstract
Itaconate is a mammalian antimicrobial metabolite that inhibits the isocitrate lyases (ICLs) of Mycobacterium tuberculosis. Herein, we report that ICLs form a covalent adduct with itaconate through their catalytic cysteine residue. These results reveal atomic details of itaconate inhibition and provide insights into the catalytic mechanism of ICLs.
- This article is part of the themed collection: Tuberculosis
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