Issue 30, 2024
From the journal:

Chemical Communications

Locus-specific detection of pseudouridine with CRISPR-Cas13a†

Mei Sun,a   Xin Fang,a   Bingqian Lin, ORCID logo a   Jing Mo, ORCID logo a   Fang Wang, ORCID logo b   Xiang Zhou ORCID logo ac  and  Xiaocheng Weng ORCID logo *a  

* Corresponding authors

a College of Chemistry and Molecular Sciences, Department of Clinical Laboratory of Zhongnan Hospital, Key Laboratory of Biomedical Polymers-Ministry of Education, Wuhan University, Wuhan, Hubei, P. R. China
E-mail: xcweng@whu.edu.cn
Tel: +86-027-68789746

b Wuhan University School of Pharmaceutical Sciences, Wuhan, China

c Wuhan TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P. R. China

Abstract

We combined the CRISPR-Cas13a system with CMC chemical labeling, developing an approach that enables precise identification of pseudouridine (Ψ) sites at specific loci within ribosomal RNA (rRNA), messenger RNA (mRNA) and small nuclear RNAs (snRNA). This method, with good efficiency and simplicity, detects Ψ sites through fluorescence measurement, providing a straightforward and fast validation for targeted Ψ sites of interest.

Graphical abstract: Locus-specific detection of pseudouridine with CRISPR-Cas13a

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Article information

DOI
https://doi.org/10.1039/D4CC00179F
Article type
Communication
Submitted
15 Jan 2024
Accepted
06 Mar 2024
First published
14 Mar 2024

Chem. Commun., 2024,60, 4088-4091

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Locus-specific detection of pseudouridine with CRISPR-Cas13a

M. Sun, X. Fang, B. Lin, J. Mo, F. Wang, X. Zhou and X. Weng, Chem. Commun., 2024, 60, 4088 DOI: 10.1039/D4CC00179F

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