Transformation from helical to layered supramolecular organization of asymmetric perylene diimides via multiple intermolecular hydrogen bonding

Max Planck Institute for Polymer Resea Germany. E-mail: muellen@mpip-mainz.mp Key Laboratory of Microelectronic Devices Microelectronics, Chinese Academy of Scien University of Chinese Academy of Sciences, Division of Molecular Imaging and Photoni University of Leuven, Celestijnenlaan 200 F Department of Biomedical Engineering, U 78712, USA Department of Molecular Physics, Faculty of Zeromskiego 116, 90-924 Lodz, Poland † Electronic supplementary informa 10.1039/d0sc01911a ‡ Contributed equally to this work. Cite this: Chem. Sci., 2020, 11, 4960


Introduction
The self-assembly of biological systems has inspired scientists to explore the complex overlap of intermolecular forces such as p-stacking, hydrogen bonding and dipole-dipole interactions for functional materials. 1 A typical example are polycyclic aromatic hydrocarbons (PAHs) carrying exible alkyl chains because they exhibit thermotropic properties due to selfassembly into columnar supramolecular structures. 2 The resulting packing depends upon the nature of substituents, the size and symmetry of the core and the directed non-covalent interactions of hydrogen bonding. 3 The lateral rotation between adjacent aromatic cores commonly results in a helical organization and affects the charge carrier transport along the columnar stacks due to changes in the orbital overlap. 4 The adequate combination of p-stacking and hydrogen bonding in PAHs, thus, allows one to establish a desired liquid crystalline organization within a wide temperature range.
A special case of PAHs are rylenes which consist of peri-fused naphthalenes. 5 The most prominent molecular system are perylene tetracarboxydiimides (PDIs) which assemble into different helical structures depending on their substituents and functional groups. 6 The substituents are commonly attached at the two imide positions of the PDI core in a symmetric fashion and, furnish, for instance, intermolecular hydrogen bonding and p-stacking interactions. In the case of PDI 1 helical nano-bers assemble due to hydrogen bonding between amide groups of adjacent molecules (Fig. 1). 7 The helical pitch of few tens of nanometers of the bers is attributed to the directed hydrogen bonding. Substituents with high steric demand at the two imide positions are also used to control the molecular packing. The dendritic groups of PDI 2 stimulate a lateral rotation of the molecules and induce complex helical columnar organizations depending on the length of the exible spacer between the PDI core and the dendron. The helical columns can contain tetramers of PDI as basic repeat unit, which are based on two molecules lying side-by-side in each stratum. 8 In another case, single-handed helical assemblies are generated following a cogwheel mechanism independent of the chirality of the molecules. 9 Our group has found that simple, but spacedemanding dove-tailed heptyloctyl side chains at the imides can also trigger a helical packing of PDI 3 containing 3 molecules per pitch. 10 Interestingly, an identical packing has been observed for the larger terrylene and quaterrylene diimides substituted by the same side chains. 11 When the hydrocarbon side chains are changed to longer triethyleneglycols, PDI 4 arranges in a staggered fashion. 12 An asymmetric substitution of the PDI core at the imide positions by alkyl side chains and hydrogen bonding functions offers further opportunities to tune the supramolecular selfassembly. Indeed, while the solid-state organization of symmetrically substituted PDIs has been well studied, little information is available for asymmetrically substituted PDIs in spite of few reported examples. The asymmetrical functionalization of PDI has great impact on the size, aspect ratio, and surface properties of the resulting self-assembled entities. A broad range of structures was observed for PDI 5 derivatives differing in the length of the alkyl spacer between the imide position and various pendant silylated alcohol functionalities. 13 As another rare example of asymmetric PDIs, compound 6 bears a benzyl moiety with two chiral sec-butoxy substituents and a dodecyl side chain. 14 The introduction of chiral substituents induces the formation of single-handed nano-coils and -bers self-assembled aer solution deposition. 15 Introduction of side groups capable of non-covalent interactions can lead to further complex supramolecular structures of asymmetric PDIs as in the case of 7. This molecule contains a branched alkyl side chain and a carboxylic acid group leading to intermolecular hydrogen bonding in non-polar solvents and nally to catemers between molecules in neighboring stacks. 16 Flash-photolysis time-resolved microwave conductivity measurements revealed a charge carrier mobility of 0.24 cm 2 V À1 s À1 for this compound. In solution, the unsubstituted imide of the asymmetric PDI 8 is capable of two-fold hydrogen bonding with the carbonyl group of a neighboring core. 17 The resulting organization consists of hydrogen-bonded dimers that are stacked in columns. The formation of hydrogen bonds and the self-assembly of asymmetric PDI can be adjusted by the polarity of the solvent. 18 This dimer assembly signicantly differs from the intracolumnar hydrogen bonding of the symmetrically substituted PDI 1 and resembles biological supramolecular structures such as DNA base pairing. While the solution self-assembly of asymmetrically substituted PDIs has been extensively studied, the solidstate organization remained less explored.
The structural study in this work reveals that monosubstituted PDI 9 with dove-tailed side chain arranges in hydrogen-bonded dimers in the bulk. However, the supramolecular organization strongly depends on the type of hydrogen bonding that is controlled by thermal annealing. The main structural information is derived from solid-state nuclear magnetic resonance (SSNMR) and wide-angle X-ray scattering measurements. Aer annealing at low temperatures, robust pstacking interactions between aromatic PDI cores and hydrogen bonding between carbonyl and imide groups facilitate a helical self-organization of V-shaped dimers into columnar stacks. Higher annealing temperatures result in improved molecular order and planarization of the PDI dimers. Additional intermolecular hydrogen bonding between carbonyl and aromatic H(Ar) initiate a reorganization from the helical to a thermodynamically favorable layered supramolecular structure. The occurrence of multiple hydrogen bonding allows to further program and construct other supramolecular assemblies consisting of a high variety of aromatic building blocks.

Differential scanning calorimetry (DSC)
DSC was measured using a Mettler DSC 30 setup at a scan rate of 5 C min À1 .

UV-vis absorption
Thin lms for UV-vis absorption were spin-coated from a 3 mg mL À1 chloroform solution onto cleaned quartz substrates, and then thermally annealed at 100 C or 250 C for 30 min in a nitrogen glovebox. The corresponding UV-vis spectra were recorded at room temperature through a PerkinElmer Lambda 950 spectrophotometer.

Fourier transform infrared spectroscopy (FTIR)
FTIR spectra of spin-coated lms was measured with a Nicolet 730 FTIR spectrometer. The FTIR spectra were averaged over three different separate lms. The integration time for the IR measurements was 800 s. Average IR intensity values between the frequencies of 2120 cm À1 and 2140 cm À1 of each averaged FTIR spectra were used for the background subtraction.

Solid-state nuclear magnetic resonance (SSNMR)
All SSNMR experiments were performed with a Bruker Avance III console operating at 850.27 MHz 1 H Larmor frequency and ambient temperature using commercial double resonance MAS NMR probes designed for zirconia rotors with 2.5 mm out diameter. If not stated differently, 1 H MAS NMR measurements were performed at 25 kHz MAS spinning frequency, whereas 13 C CP-MAS NMR measurements were recorded at 18 kHz MAS spinning frequency, 3 ms Lee-Goldburg CP contact time and 100 kHz high power sweep-frequency TPPM dipolar decoupling. The 1 H double quantum-single quantum correlation NMR spectra were acquired at 25 kHz MAS and at ambient temperature using the back-to-back double quantum recoupling scheme for 2 rotor periods double quantum excitation and reconversion.

Two-dimensional wide-angle X-ray scattering (2DWAXS)
2DWAXS setup consists of the Siemens Kristalloex X-ray source (copper anode X-ray tube, operated at 35 mV/20 mA), Osmic confocal MaxFlux optics, two collimating pinholes (1.0 and 0.5 mm Owis, Germany) and an antiscattering pinhole. 2DWAXS pattern was recorded on a MAR345 image plate detector (Marresearch, Germany). The sample was prepared by lament extrusion using a home-built mini-extruder, and then positioned perpendicular to the incident X-ray beam and vertical to the 2D detector.

Film deposition by zone-casting
Zone-casting was utilized to deposit thin lms of 9. The Si/SiO 2 substrate was moved at a controlled speed and temperatures for both solution and substrate. The concentration of PDI 9 is 0.5 mg mL À1 . The optimized processing parameters are 1,1,2,2tetrachloroethane (TCE) as solvent, solution/substrate temperatures of 70/60 C, and moving speed of 5 mm s À1 . The details of optimization are shown in Fig. S7. † Aer lm fabrication, thermal annealing at 100 C for 0.5 h is applied to remove residual solvent.

Topography characterizations
The topography of deposited lms was characterized by a Digital Instruments Nanoscope IIIa atomic force microscopy (AFM) in tapping mode and transmission electron microscopy (TEM, FEI Tecnai F20). To gain information on the molecular organization of 9 in the zone-cast lms, selected-area electron diffraction (SAED) patterns were recorded by a Philips CM 12 electron microscope.

Scanning tunneling microscopy (STM)
Stock solution of PDI (8.3 Â 10 À4 M) was prepared by dissolving appropriate amount of solid in 1,2,4-trichlorobenzene (Sigma-Aldrich, >99%). The stock solution was diluted further to make concentration series. All STM experiments were performed at room temperature (21-23 C) using a PicoLE (Agilent) machine operating in constant-current mode with the tip immersed in the supernatant liquid. STM tips were prepared by mechanically cutting a Pt/Ir wire (80%/20%, diameter 0.2 mm). Prior to imaging, a drop of warm PDI solution was placed onto a freshly cleaved surface of highly oriented pyrolytic graphite (HOPG, grade ZYB, Advanced Ceramics Inc., Cleveland, USA). The experiments were repeated in 2-3 sessions using different tips to check for reproducibility and to avoid experimental artifacts, if any. For analysis purposes, recording of a monolayer image was followed by imaging the graphite substrate underneath it under the same experimental conditions, except for increasing the current and the lowering the bias. The images were corrected for dri via Scanning Probe Image Processor (SPIP) soware (Image Metrology ApS), using the recorded graphite images for calibration purposes, allowing a more accurate unit cell determination. The unit cell parameters were determined by examining at least 4 images and only the average values are reported. The images are Gaussian ltered. The imaging parameters are indicated in the gure caption: tunneling current (I set ), and sample bias (V bias ). The molecular models were built using Hyperchem™ 7.0 program.

Results and discussion
3.1 Formation of hydrogen bonding PDI 9 was synthesized according to literature, 19 and the presence of its hydrogen bonding in solid-state is rstly conrmed by Fourier-transform infrared spectroscopy (FTIR) of lms before and aer annealing at 100 C and 250 C. The later annealing temperature was taken as maximum also for all other experiments, except SSNMR (see below), since evaporation of the material occurs above this point. The peak intensity of the vibration associated with hydrogen bonded carbonyl (-C]O) groups slightly increases and is red shied from 1690 cm À1 to 1680 cm À1 aer annealing at 250 C (Fig. 2a). Additionally, the low frequency part of the peak at 1680 cm À1 to 1695 cm À1 increases. These changes in peak position and intensity suggest increased hydrogen bonding mediated by carbonyl groups. Contrary to these observations, the intensity of the stretching mode related to hydrogen bonded imide (-NH) groups centered at 3200 cm À1 decreases and those attributed to non-hydrogen bonded (or free) imide groups at 3381 cm À1 increases aer annealing the sample at 250 C (Fig. 2b). These results indicate that the hydrogen bonding of the imide groups is weakened at higher annealing temperatures, while at the same time the noncovalent interaction by the carbonyl groups are enhanced. The moderate annealing temperature of 100 C does not initiate any change in the sample. Large changes are also evident in the UVvis absorption between lms annealed at 100 C and 250 C (Fig. S1 †). For 250 C, the spectrum is red shied and becomes less dened with broader features indicating more heterogeneous structures and improved electron coupling between the molecules. These observations will be conrmed in the structural analysis of Section 3.2 that also allows better understanding of the changes in hydrogen bonding aer high temperature annealing.
The direct visualization of the hydrogen bonding of PDI 9 is realized through scanning tunneling microscopy (STM) under ambient conditions. The molecular resolution STM image in Fig. 3a depicts that the bright features arise from rows of oval shaped structures and always appear in pairs. These oval features correspond to the aromatic backbones of 9 and their pairwise occurrence indicates the presence of hydrogen-bonded dimers on the surface as expected from the hydrogen bonding sites between carbonyl and imide groups on 9. Interestingly, adjacent dimers are shied by almost half a molecule with respect to each other suggesting their additional interactions via carbonyl and aromatic H(Ar) (Fig. S2 †). This type of hydrogen bonding has also been reported for unsubstituted for perylene-tetracarboxylic-dianhydride. 20 The molecular rows run along one of the main symmetry axes of the HOPG substrate. The bright columns are separated by relatively darker narrow troughs where the alkyl chains are adsorbed. The alkyl chains could not be resolved, possibly in view of their mobility on the surface within the time scale of STM measurement. Molecular models based on the unit cell parameters indicate that there is not enough space in between adjacent molecules for the adsorption of both alkyl chains per molecule (Fig. 3b). The chains that are not adsorbed on the surface could be simply back-folded in the supernatant solution phase. A peculiar feature of the monolayer is the presence of defects in the form of kinks (green arrow, Fig. 3a) along the molecular rows. A careful inspection indicates that these defects do not interrupt the carbonyl-imide hydrogen bonding between individual PDI molecules (dashed white ovals, Fig. 3a). These insights provide important information between which groups of the PDI 9 molecules hydrogen bonding is formed.

Structural analysis of supramolecular assembly
To correlate the results from FTIR and STM with the organization in bulk, the hydrogen bonding promoted self-organization of 9 is further explored by SSNMR. Since for these measurements the PDI samples were contained in a sealed holder, higher temperatures of 310 C and 340 C were additionally feasible being associated with phase transitions from DSC and annealing experiments (Fig. S3 and S4 †). In the 1 H MAS NMR spectra of the sample annealed at 100 C, the aromatic signal is relatively broad (Fig. 4a). Upon further annealing, the aromatic 1 H signal narrows gradually in the spectrum and shis towards lower ppm values, indicating an improved p-stacking and intermolecular order. The 1 H signal of the hydrogen bonded sites, seen as a weak shoulder around 11 ppm for the sample annealed at 100 C, becomes better dened and shis towards higher ppm values. This demonstrates the formation of better-  ordered or additional hydrogen bonds aer annealing at elevated temperatures. In the 13 C CP-MAS NMR spectra the enhanced self-organization is also observed via the improved chemical shi resolution of the aromatic and carbonyl sites of the PDI cores (Fig. 4b). It should be pointed out, that the number of the observed 13 C NMR signals exceeds the number of chemically distinct sites in the molecule, indicating the importance of the local packing for the chemical shis in SSNMR. In contrast to the PDI core, the 13 C signals of the aliphatic side chains show only a weak line narrowing with increasing annealing temperature, which might be attributed to the improved phase separation between the PDI cores and the aliphatic side chains due to the higher stacking order of the PDI cores.
Further information about the hydrogen bonding is obtained by two-dimensional 1 H double quantum (DQ) correlation NMR spectra recorded at ambient temperature aer the different annealing stages of the sample at ambient temperature. In Fig. 5a-d, pairwise correlations between 1 H sites in close spatial proximity are monitored, so that the formation of hydrogen-bonded dimers can be distinguished from spatially isolated hydrogen bonding 1 H sites. For perfectly symmetric hydrogen-bonded dimers with two equivalent hydrogen-bonded proton sites in close spatial proximity, a DQ correlation signal on the diagonal of the spectrum would be expected. On the other hand, a spatially isolated hydrogen bonding site would not be monitored, because DQ coherences can only be excited between spin 1 2 nuclei with signicant dipolar couplings and all other signals are cancelled by an appropriate phase cycle. Weak distortions of the hydrogen-bonded dimers, where one of the hydrogen bonds is elongated while the second bond is slightly shortened, will cause a splitting of the signal symmetric to the diagonal. On the other hand, heterogeneous packing environment with neighboring PDI molecules will lead to a heterogeneous distribution of isotropic chemical shis seen as an elongation of the DQ signal parallel to the diagonal of the spectrum. The 1 H DQ correlation spectrum of the weakly annealed sample shows both features described above, the splitting due to distortions of the dimers, and an elongation along the diagonal due to columnar stacking heterogeneities resulting from variations of the helical pitch and p-stacking distances (Fig. 5a). Upon further annealing, the molecular order of the PDI dimers is substantially improved as seen by the renement of spectral features in the 13 C CP-MAS NMR spectrum assigned to the PDI core (110-180 ppm, Fig. 4b). The elongation along the diagonal as well as splitting of the NH proton signal vanishes with increasing annealing temperature, while at the same time a new DQ coherence involving hydrogen bonding protons at 12.2 and aromatic protons at 9.9 ppm (Fig. 5b-d) evolves. This observation conrms the formation of hydrogen bonding between aromatic proton and carbonyl sites of neighboring PDI cores and suggests that the planar packing arrangement (Fig. S2 and S5 †), monitored by STM studies as surface structure, might resemble the bulk molecular packing aer high temperature annealing. Moreover, it shows that the temperature dependent chemical shi of the hydrogen-bonded proton sites, discussed in Fig. 4, cannot be attributed to a thermal restructuring of the NH-carbonyl hydrogen bonding, but originates from the formation of hydrogen bonding between aromatic proton and carbonyl sites of the PDI cores as also found by FTIR.
Aer annealing the sample at 340 C, the intensity of the mixed coherence has approximately twice the intensity of the pure NH proton coherence, which is expected for the layered bulk structure. In Fig. 5e, the carbonyl region of a 13 C-1 H FSLG-CP correlation NMR spectrum of the 100 C annealed sample reveals only ill-dened correlation signals to aromatic proton sites. In contrast, the same correlation spectrum of 9 aer annealing at 340 C, given in Fig. 5f, shows numerous well-dened correlations due to the improved molecular order. The new correlation signals at 13 C chemical shis above 165 ppm with hydrogen bonding protons, originate from carbonyl sites hydrogen-bonded to aromatic protons, which are not present in the initial helical packing of the sample. In summary, the solid-state NMR results indicate the formation of a new bulk structure on the molecular level driven by additional hydrogen bonding upon high temperature annealing consistent with the layered surface structure observed in the STM images and FTIR results.
To quantify the reorganization of PDI 9 upon annealing twodimensional wide-angle X-ray scattering (2DWAXS) is performed for extruded ber samples up to the annealing temperature of 250 C. The patterns in Fig. 6 conrm a signicant transition of the supramolecular structure with thermal annealing observed by SSNMR. Fig. 6a exhibits the 2DWAXS pattern aer annealing at 100 C. Equatorial reections in the small-angle range (assigned as c1 in Fig. 6a and S6 †) indicate columnar structures aligned along the ber sample and arranged in a square-like lattice with the parameter of a ¼ 4.18 nm. This value is in accordance with the calculated length of a PDI dimer with side chains of around 4.60 nm. Higher order reections, up to the third order, are characteristic for a long-range molecular order. Interestingly, these scattering intensities exhibit an azimuthal splitting with maxima at g ¼ 11 (Fig. S6 †) with respect to the equatorial plane suggesting a relative shi of 0.67 nm of the columns along the ber axis towards each other. In the wide-angle range, reections located at the off-meridional plane (assigned as a1 in Fig. 6a) correspond to the p-stacking distance of 0.34 nm of tilted PDI molecules. From the azimuthal position of the reection a molecular tilt angle of b ¼ 48 with respect to the columnar axis is determined. As illustrated in Fig. 7c, the dimers, formed with the assistance of hydrogen bonding dominated solely by interactions between -N-H and -C]O groups, adopt a V-shape arrangement with titled PDI molecules. Additional low intensity meridional reections (assigned as b1, Fig. 6a) in the smallangle region imply a helical organization of the dimers with a pitch of 1.41 nm containing three dimers. 4,8a,21 Thereby, the dimers are rotated by around 60 to the next neighbor within the stack to ensure an identical positional order between each 4 th dimer (Fig. 7b). The position and shape of the reections in the pattern suggest a helix structure, similar to DNA, along the ber direction. Note that the higher order reections possess weak intensity characteristic to short-range order.
Aer annealing at 150 C, the molecular order is noticeably enhanced, as evident from the larger number of distinct and high intensity reections. The annealing leads to a slight increase in the lattice parameter of the square-like unit cell to 4.26 nm. The azimuthal integration of the intercolumnar reection (assigned as c2, Fig. 6b) reveals also a splitting with its maximum located at g ¼ 25 with respect to the equatorial plane. This might suggest that the columns remain aligned  parallel along the ber sample, but are translated by a larger value of 1.98 nm. From the split wide-angle reections (a2, Fig. 6b) a p-stacking distance of 0.34 nm and molecular tilting of b ¼ 25 are derived. This tilted molecular arrangement is conrmed by the additional reection a2 0 that is at the same azimuthal angle as a2 at a d-spacing of 0.37 nm for the intracolumnar period. The helical pitch of 1.46 nm remains almost unchanged as indicted by the small-angle scattering intensity b2 in Fig. 7b still including 3 dimers with a molecular rotation angle of 60 .
The sample annealed at 250 C reveals an additional type of organization and signicantly higher crystallinity in bulk as evident by the large number of distinct reections (Fig. 6c). The shape of the main reections in the small-angle range implies polymorphism with up to 4 different unit cells which vary only slightly from each other. The main identied unit cell is rectangular with a ¼ 3.80 nm and b ¼ 2.37 nm (c3, Fig. 6c). In this lattice the dimers are arranged in a layered order as shown in Fig. 7d analogous to the monolayer displayed by STM (Fig. 3). The driving force for this reorganization is additional intermolecular hydrogen bonding between carbonyl and aromatic H(Ar) which were found by FTIR and SSNMR. These interactions induce a planar molecular arrangement within the dimers (b ¼ 0 ). During this rearrangement, a certain amount of the hydrogen bonding between imide and carbonyl groups breaks causing an intensity decrease of the IR peak for hydrogenbonded N-H. Since at the same time, new hydrogen bonded carbonyl is established, the intensity of this IR peak remains unchanged. The wide-angle range reections, a3, located at the meridional plane are assigned to the molecular packing. However, the high number of reections in this range does not allow to unambiguously distinguish the p-stacking distance but indicates a slight tilting of the entire dimer towards the stacking axis resulting in a herringbone structure (side view illustration of the lattice in Fig. S7 †). Additionally, a minor phase of a hexagonal unit cell with a hex ¼ 4.17 nm was also identied from the equatorial reections of the 2DWAXS pattern. This hexagonal phase might be related to a not fully accomplished transition from helical to layered ordering as also revealed by SSNMR.
The good solubility of PDI 9 in common solvents allows the processing in solution to control the microstructure. Among solution processing techniques, zone-casting offers unique advantages such as a broad variation of the processing parameters for the uniaxial alignment of conjugated molecules. 22 For PDI 9, the optimized zone-casting conditions are TCE as solvent, solution/substrate temperatures of 70/60 C, and moving speed of 5 mm s À1 , by which well-dened morphologies are achieved (Fig. S8 †). As shown in Fig. 8a, the self-assembly of PDI 9 is directed by zone-casting with the formation of uniaxially aligned ber-like superstructures along the casting direction. These oriented nanobers reach few micrometers in length which might be attributed to intermolecular p-stacking interactions. A more detailed picture is obtained by TEM. Fig. 8b exhibits that individual nanobers essentially consist of a bundle of smaller, ca. 3.8 nm thick columns that are also oriented in the zone-casting direction. This distance is in good agreement with the dimension of hydrogen-bonded dimer of PDI 9. Further structural information on these well-dened columns is obtained from selected area electron diffraction (SAED) in Fig. 8c. Two split reections correspond to spacings of 0.34 nm and 0.37 nm and are related to the packing of the tilted PDI molecules in the stacks as illustrated in Fig. 7c. The reection splitting is consistent with 2DWAXS results in Fig. 6a for the annealing temperature of 100 C.

Conclusion
The supramolecular structure of monosubstituted PDI 9 with dove-tailed side chain is investigated in solid-state aer thermal annealing at various temperatures. Due to hydrogen bonding between carbonyl and imide groups, the PDI 9 molecules form dimers. The initial organization consist of a helical columnar structure of p-stacked and V-shaped dimers. Annealing at high temperatures induces a transformation into a thermodynamically favorite state of a highly ordered layered structure in that the dimers are quasi planarized and are additionally hydrogen-bonded through carbonyl and aromatic H(Ar) interactions. This type of intermolecular hydrogen bonding is a rare example that can play a fundamental role in the solid-state supramolecular organization of perylene tetracarboxydiimides. The transformation between two distinct assemblies is driven by the occurrence of multiple interactions and might be accompanied by changes in the functionality of the material.

Conflicts of interest
There are no conicts to declare.