Combining hydrophilic and hydrophobic environment sensitive dyes to detect a wide range of cellular polarity

Ratiometric polarity sensitive probe (RPS-1) contains two dyes of same absorption but different emissions utilized in comprehensive and quantitative detection of wide range of intracellular polarity.


Introduction
In the intracellular environment, polarity, viscosity, temperature, redox status, and pH parameters are critical for the initiation and maintenance of the physical and chemical behaviors of biomolecules, because their distribution, spatial arrangement, and composition within the cell are heterogeneous. 1,2 Intracellular polarity, in particular, is key to various cellular processes such as cell proliferation, immune system regulation, increases in the number of local membranes, stimulation of cell migration, and vectorial transport of molecules across the cell layer. 3,4 Each organelle of the cell has an optimal polarity depending on its role and the polarity changes in real time as the intracellular environment changes. [5][6][7] The pathological activity of the cell changes with its polarity, and abnormal polarity is related to diabetes, neurological diseases, and cancer. [8][9][10][11] Therefore, detecting cellular polarity is important in research on pathological and biological phenomena.
The only way to observe intracellular polarity is to use optical imaging; therefore, various uorescent probes have been developed to detect the polarity of microenvironments. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Most reported polarity probes are based on intramolecular charge transfer (ICT) and show shis in emission wavelengths based on changes in the polarity of a cell's surroundings. However, most of these probes are solvatochromic and have disadvantages including the fact that the uorescence efficiency decreases sharply as the solvent polarity increases, limiting the detection range in hydrophobic environments. In addition, the reported probes can only detect specic structures in a cell, such as the mitochondria, 12,13 lysosomes, 14-17 endoplasmic reticulum (ER), 18-20 lipid droplets, 21-23 and cell membrane. [24][25][26] Therefore, these probes are limited to detecting the polarity of a particular organelle or a limited zone and have difficulties in imaging the distribution of polarity over the entire cell. Recently, a polarity sensitive probe for detecting lysosomes and lipid droplets was reported. 27 Even though the probe showed good selectivity, its applications are limited because two different excitation wavelengths must be used to observe each organelle, making it difficult to show the polarity distribution in a cell in real time and thus to quantify the polarity of the organelles. To maintain and regulate appropriate cellular activities, multiple organelles exchange materials and transmit intracellular signals. 28,29 Therefore, studying complex subcellular organelle interactions requires a highly sensitive polarity probe that labels more than two organelles and can quantitatively detect a wide range of cellular polarities.
Here, we introduce a ratiometric probe (RPS-1) that overcomes the limitations described above. We rst synthesized D-p-A type dipolar compounds by treating aminosalicylaldehyde with indolium and a benzothiadiazole acceptor expecting to produce ICT-based dyes, denoted as Dye1 and Dye3 (Scheme 1). Dye3 was characterized as an ICT-based dye whose uorescence largely shied to the red-region, but decreased dramatically following an increase in solvent polarity (see below, Fig. S6 †). Interestingly, Dye1 showed an "off-on" characteristic in hydrophobic and hydrophilic solvents as its structure underwent a ring opening/closing cycle. To cover a wide range of polarities, we formed a ratiometric probe that combined these two dyes, which were sensitive to hydrophilic and hydrophobic environments (Dye1 and Dye3), respectively. This probe had different emission windows and could be excited with a single wavelength source. The uorescence intensity ratio of RPS-1 was dramatically changed between the yellow and red windows, depending on the polarity, and was highly correlated with the E N T value, a parameter that indicates the polarity of a solvent. Therefore, because RPS-1 could quantitatively detect the polarity of various organelles in cells, the probe revealed that lysosomes were the most polar organelle in cells and that lipid droplets were the most non-polar.

Results and discussion
Polarity probes were synthesized by condensing salicylaldehyde and 1,2,3,3-tetramethyl-3H-indolium iodide under ethanol (EtOH) conditions. The detailed synthesis method, yield, and NMR for each intermediate are described in the ESI. † The synthesized Dye1 was measured for absorbance and uorescence intensity using solvents with various polarities ranging from toluene to water (Fig. 1). The charge transfer of Dye1 was blocked in the nonpolar solvent due to its closed-ring structure and it absorbed in the short wavelength region at 328 nm. However, in solvents with a high polarity, such as those above EtOH, Dye1 opened the ring of the closed structure and increased the conjugation bridge, thus recovering the charge transfer from the diethylaniline to the indolium salt (Scheme 1b). As a result, the absorbance gradually decreased at 328 nm but increased at a long wavelength of 549 nm. Similarly, there was no uorescence intensity for Dye1 at an excitation of 552 nm in the nonpolar solvent, but strong uorescence did occur in the 573 nm region in the highly polar environment.
Dye2 was synthesized to block cyclization by introducing a methyl group into the hydroxyl group to conrm that the change in absorbance and uorescence based on the solvent polarity of Dye1 was the result of the intramolecular cyclizationopening system. Dye2 showed strong absorbance at approximately 550 nm in all solvents, regardless of their polarity, and showed no absorbance at a wavelength of 328 nm (Fig. S1 †). The uorescence intensities of Dye2 at the 552 nm excitation source were observed at 580 nm in all the solvents.
To conrm that the intramolecular cyclization and opening of Dye1 could be reversed by changing the solvent polarity, Dye1 was added to both MeOH and THF as the solvents ve times and the uorescence spectra were measured ( Fig. 1c and d). A constant change in uorescence was observed under each set of conditions tested; thus, it was conrmed that the intramolecular cyclization and opening for Dye1 were reversible with the change in solvent polarity.
The 1 H NMR spectra were analyzed in nonpolar and polar solvents to further demonstrate the cyclization-opening system of the polarity probes (Fig. 2). In the non-polar CDCl 3 solvent, Dye1 was a closed structure and the intramolecular chiral center was present. Due to this, the two gem-dimethyl groups in the spirocyclic form, with d 1.14 and 1.32 ppm, were well separated. However, in the polar solvent environment of CD 3 OD : D 2 O ¼ 1 : 1, the spirocyclic form of Dye1 underwent an intramolecular structural change in the opened structure and the chiral center disappeared. The separated gem-dimethyl was observed as one peak at d 1.72 ppm. At the same time, the amine of indoline became positively charged as the polarity of the solvent increased. As a result, the methyl of the amine was shied from d 2.74 ppm to d 3.71 ppm. In the medium polarity environment of CDCl 3 : CD 3 OD ¼ 1 : 1, the NMR data showed that both the closed and opened structures coexist. A clear C]C double bond cis proton was observed in the aromatic region; it was due to the spirocyclic ring in the nonpolar environment (J ¼ 10.3 Hz). In the polar environment, it was difficult to observe a clear trans proton but there was a clear and large downeld shi due to the strong electron withdrawing effect. The NMR peaks and chemical structures of Dye1 for each polarity environment were assigned (Fig. S2 †). The absorbance and uorescence spectra of the probes in the range of pH 4 to pH 10 were recorded to determine whether the polarity probe was affected by pH (Fig. S3 †). The results showed that all polarity probes were always in the open form in aqueous solution, regardless of the change in pH.
Each polarity probe was incubated in HeLa cells for 30 min. Under the 552 nm excitation wavelength, Dye1 and Dye2 emitted bright uorescence, but the intracellular locations of each probe were different. Dye1 tended to stain a specic vesicle in the cell, whereas Dye2, which was always in the open form, stained a wide area in the cell, which might be mitochondria (Fig. 3). As a result of the co-localization experiments with each of the polarity probes with LysoTracker Green, Dye1 signicantly overlapped with a Pearson coefficient value of 0.94, suggesting that the organelles where this probe was located were lysosomes (Fig. 3a). However, Dye2 had a low Pearson coefficient with LysoTracker (Fig. S5 †), which was not overlapping, and instead showed a high overlap with MitoTracker Green (Fig. 3b). The mitochondrial inner membrane is known to have a proton concentration gradient for ATP synthesis and a negative charge of about À180 mV. 30 As a result, small molecules with positive charge tend to accumulate inside the mitochondria due to electrostatic attraction. Therefore, Dye2, which was positively charged due to the opened structure, was located in the mitochondria for the same reason. However, it has been reported that cyclized indolines like Dye1 can be located in lysosomes in a tertiary amine form that can act as a proton receptor. 31 These results suggested that either Dye1 preferred to be located in the lysosomes or the probe was distributed at various locations within the cell but only the lysosomes could be seen because the lysosomal polarity was specically higher than other intracellular compartments. The turn-on based probes inuenced the uorescence intensities by various factors such as local concentration, intracellular environment, and imaging conditions. Therefore, to quantitatively conrm the intracellular polarity distribution, a ratiometric probe, whose uorescence ratio changed according to the environmental polarity, was required.
The benzothiadiazole derivative (Dye3) had an absorption wavelength similar to that of Dye1; however, due to a larger stoke shi, the uorescence emitted in the long wavelength region of the near infrared (NIR) region minimized the uorescence interference between the two dyes. In contrast to Dye1, Dye3 was a turn-off probe whose uorescence intensity decreased as the solvent polarity increased (Fig. S6 †). Dye1 and Dye3 were combined by introducing the piperazine linker, and the two dye uorescence intensities responded oppositely according to the environmental polarity. Through this, we synthesized a ratiometric polarity probe (RPS-1), which had two uorescence spectra with one excitation source (Scheme 1). RPS-1 showed a weak absorbance at 510 nm in non-polar solvents such as toluene and ether, based on Dye3, and strongly increased the absorbance at 550 nm in polar solvents such as MeOH and water, based on Dye1 (Fig. 4a). Moreover, as the polarity increased, the uorescence intensity near 650 nm in the nonpolar solvent gradually decreased and the uorescence around 580 nm increased (Fig. 4b). The two regions for the ratio uorescence measurement were dened as 565-585 nm (F yellow ) and 630-680 nm (F red ), and there was a high correlation between the uorescence ratio of F yellow /F red and the E N T value, which is the solvent polarity parameter (R 2 ¼ 0.993, Fig. 4c). The photophysical properties of RPS-1 are summarized in Table S3. † Additionally, RPS-1 showed reversible changes in the ratio of F yellow /F red depending on the solvent polarity, a behavior similar to that of Dye1 (Fig. 4d). The ratio of RPS-1 was almost constant  in various pH ranges (Fig. S7 †) and in the presence of other biological metabolites, including reactive oxygen and nitrogen species, cationic and anionic amino acids, glutathione, and enzymes (Fig. S8 †). Additionally, RPS-1 showed high photostability under the imaging conditions used (Fig. S9 †), with negligible cytotoxicity (Fig. S4 †).
Ratiometric images were acquired with two channels, F yellow and F red , to conrm whether RPS-1 reected the difference in polarity in various compartments within the cell (Fig. 5). The results of HeLa cells labeled with RPS-1 (3 mM) for 30 min surprisingly showed a clear difference between the uorescence of the F yellow channel and the F red channel ( Fig. 5a and b). Similar to the cell image of Dye1, the F yellow channel showed a strong uorescence in a specic vesicle in the cell. However, the uorescence of the F red channel was observed in other vesicles, in addition to the same F yellow vesicle, and stained with strand formation throughout the cell. When the uorescence images of F yellow and F red were treated with a pseudocolored ratiometric image (F yellow /F red ), the distribution of the polarities of each region of the cell could be conrmed at a glance (Fig. 5c). All the intracellular polarity images of RPS-1 were conrmed by co-localization experiments with various commercial organelle markers (Fig. 6). In the ratiometric images, the red region with the highest polarity overlapped with the lysosome, while the blue region with the lowest polarity overlapped with the lipid droplet marker. The green region with moderate polarity overlapped with both mitochondria and ER markers, and we dened this region as the cytoplasm. In the enlarged images, the lysosome, cytoplasm, and lipid droplet regions were more clearly distinguished, and the blue-colored lipid droplets in the ratiometric image corresponded exactly to the regions of visually observable lipid droplets in the bright-eld image (Fig. 5d and e).   In addition to HeLa cells, the distribution of intracellular polarity in various cells such as Chang, Huh7, and SW837 was observed; the polarity of the lysosomes was observed to be the highest in all cells (Fig. 7). The F yellow /F red uorescence ratios of lysosomes in the Chang, Huh7, SW837, and HeLa cells were 1.9, 1.7, 1.9, and 1.7, respectively. The polarity of lysosomes in all cells was as high as the value between those of methanol and water (Fig. 4c). Conversely, the lipid droplets had lower polarity compared to other organelles. The F yellow /F red uorescence ratio of the lipid droplets in Huh7, SW837, and HeLa cells was 0.15, 0.24, and 0.22, respectively, while that of Chang cells was 0.43, which was higher than that of the other cells. This is consistent with previous studies that showed that the polarity of lipid droplets of cancer cells is lower than that of normal cells due to the specic lipid metabolism of cancer cells. 21,32 The cytoplasmic polarity value was between those of the lysosomes and lipid droplets, and the F yellow /F red uorescence ratio values in the cytoplasm of Chang, Huh7, SW837, and HeLa were 1.0, 0.87, 0.91, and 0.81, similar to that of 2-PrOH. In particular, the F yellow /F red uorescence ratios provided information about the polarity of solvents similar to that of lysosomes (1.7-1.9, MeOH and water), cytoplasm (0.81-1.0, 2-PrOH), and lipid droplets (0.15-0.43, DMF and EA). Consequently, intracellular polarity was heterogeneous and the polarity of each organelle gradually decreased from lysosomes, to cytoplasm, to lipid droplets.

Conclusions
We developed Dye1, in which the absorbance and uorescence intensity changed through intramolecular cyclization-opening according to solvent polarity. Dye1 is the rst hydrophilic environmentally selective turn-on probe in the spiropyran series, in which the structure of the molecule itself changes reversibly in response to polarity. We synthesized a hydrophobic and hydrophilic combined ratiometric probe, RPS-1, in which the intensity of the two uorophores was reversely changed by polarity by introducing Dye3, whose uorescence decreased as the solvent polarity increased. By connecting the two dyes with similar absorption but different solvatochromic shis, RPS-1 was able to measure the difference in uorescence ratios (F yellow /F red ) according to polarity using one excitation wavelength and quantitatively detect the change in polarity over a wide range, from toluene to water. RPS-1 was stained in various organelles in the cell and showed a difference in polarities in various regions of the cell at a glance. These results suggested that RPS-1 could detect a wide range of intracellular polarity changes sensitively and quantitatively, and conrmed that the polarity of the lysosomes was the highest in the cell. This new approach of linking two dyes with completely different characteristics resulted in a new ratiometric polarity sensing dye, RPS-1, that could provide useful information to biomedical research.
Cell images. Each cell was incubated for two days before imaging. Streptomycin (100 mg mL À1 ), penicillin (100 units per mL), and 10% fetal bovine serum were added to all culture media. The culture medium was replaced with a serum-free medium and each polarity probe was stained for 30 min. Live cell imaging was performed using a live-cell instrument (Chamlide IC) to maintain appropriate temperature, humidity, and pH for long term exposure. Ratiometric image processing and analysis were carried out using MetaMorph soware.
Co-localization experiments. Experiments were carried out by co-staining with polarity probes and each commercial organelle tracker (LysoTracker Green DND-26 for lysosomes, BODIPY 493/503 for lipid droplets, MitoTracker Green FM for mitochondria and ER-Tracker Green for the endoplasmic reticulum). The excitation wavelengths were 488 nm (organelle trackers) and 552 nm (polarity probes). Pearson's colocalization coefficient (A) was calculated using AutoQuant X2 soware.

Conflicts of interest
There are no conicts to declare.