Isolation and synthesis of cryptosanguinolentine (isocryptolepine), a naturally-occurring bioactive indoloquinoline alkaloid

Cryptosanguinolentine (isocryptolepine) is one of the minor naturally-occurring monomeric indoloquinoline alkaloids, isolated from the West African climbing shrub Cryptolepis sanguinolenta. The natural product displays such a simple and unique skeleton, which chemists became interested in well before it was found in Nature. Because of its structure and biological activity, the natural product has been targeted for synthesis on numerous occasions, employing a wide range of different strategies. Hence, discussed here are aspects related to the isolation of isocryptolepine, as well as the various approaches toward its total synthesis.


Introduction
It is now common knowledge that aer millions of years of coexisting under different external pressures (territory, food, predators, etc.), the communities of organisms sharing the same ecosystem underwent co-evolution as a survival strategy. As a result, some of their secondary metabolic routes suffered changes over time, gaining the ability to become resistant to their environment by producing certain low molecular weight metabolites. These small molecules are collectively known as natural products.
It has been found that the natural products may act as small ligands for certain macromolecular targets found within the living organisms or, more oen, within other living organisms of the same ecosystem. 1 In this way, the natural products assist the producing organism to sustain the hostilities of the environmental pressures and survive. Interestingly, many human proteins contain structural domains that are very similar to some of the macromolecular targets affected by natural products; therefore, it was not unexpected to nd out that the small molecule natural products originally produced with a different aim (reproduction, signaling, communication, defense), can Elida N. Thobokholt was born in Rosario (Santa Fe, Argentina) and studied at the School of Biochemical and Pharmaceutical Sciences at the National University of Rosario, where she earned her BS degree in Food Science and Technology in 2018. Shortly aer, she joined the group of Dr Kaufman as a Doctoral student at IQUIR. Currently, she is developing novel approaches to the synthesis of naturally occurring indoloquinoline alkaloids, under the supervision of Drs Bracca and Larghi.
Enrique L. Larghi was born in Rosario (Santa Fe, Argentina). He received his B.S. from the UNR (Argentina) in 1997 and immediately aer he moved to the UFSM (RS-Brazil) where he obtained MSc and Ph. D. degrees under the advice of Profs. Silveira (1997) and Morel (1999). Aer a short experience in the Argentine pharmaceutical industry, he returned to IQUIR, joining Prof. Kaufman's group as a postdoctoral fellow rst, and then as a staff member of CONICET. Currently, he is an Independent Researcher and Adjunct Professor at the UNR. His current interests cover the synthesis of heterocyclic natural products and their derivatives.
Their main source of indoloquinolines in Nature, Cryptolepis sanguinolenta, is a tropical scrambling and twinning shrub, indigenous to West and Central Africa. 11 The study of the plant began in the early 1950s and cryptolepine was the rst alkaloid to be isolated. 12 To date, over a dozen naturally occurring indoloquinolines have been discovered, including quinindoline (3), 7 quindoline (2), 13 quindolinone (5), 14 neocryptolepine (6), 15 cryptosanguinolentine (isocryptolepine, 7), 16 cryptolepine (8), 17 11-isopropylcryptolepine (9) 18 and cryptolepinone (hydroxycryptolepine, 10). 19 These are monomeric members of this family (Fig. 2), however, several dimeric natural indoloquinolines have also been reported. [19][20][21] The plant has long been employed in the dyeing of textiles and leather. 22 In addition, a decoction of its roots is frequently used in traditional folk medicine, to treat fevers, malaria, 23 upper respiratory infections and venereal diseases. 24 The root has been used in the Congo as a bitter stomachic, 24c and in Nigeria for the therapy of colics, rheumatism, and urogenital infections. 25 The plant has been demonstrated to possess antibacterial activity and to exert vasodilation. 21b The pharmacological activities of different indoloquinoline alkaloids, their analogs and derivatives, have been reported in several articles. The most relevant ones are their antitumoral and antiparasitic activities. 26

Isocryptolepine: its isolation and properties
Isocryptolepine was rst obtained as a degradation product during investigations on the constitution of indigo-yellow. 27 However, the heterocycle was rst isolated from a natural source (Cryptolepis sanguinolenta) in 1995 by the Bodo group as a non-crystalline substance, along with cryptolepine and quindoline. Only 5 mg were obtained from 60 g of air-dried roots ground to a ne powder (0.08% w/w). Its chemical structure was determined aer exhaustive analysis of its infrared (IR) and nuclear magnetic resonance (NMR) spectroscopic and mass spectrometric (MS) and data. 16 Interestingly, on the bases of spectral grounds, it has also been conjectured that the previously isolated and partially characterized alkaloid CSA-3 may be identical to isocryptolepine. 13 Shortly aer, the same alkaloid was isolated again by the Tackie group, from the same source in 1996, under the name cryptosanguinolentine. 28 The natural product, obtained as a yellowish residue (1.7 mg from 3.14 kg of plant roots; 0.5 Â 10 À4 % w/w), was isolated along with cryptotackieine (neocryptolepine); due to the scarcity of the material, preparative reverse-phase high-performance liquid chromatography (HPLC) and micro NMR techniques were required to conrm its structure. At a later date, the same group reported a more detailed account of their isolation, with the obtaining of another 15 alkaloids. 21a The 1 H, 13 C and 15 N NMR spectra of isocryptolepine and its protonated form were assigned as part of a systematic NMR study of four isomeric indoloquinoline alkaloids. 29 All of the NMR signals were assigned using 2D correlation techniques. Interestingly, in the 15 N NMR [a gradient selected singlequantum multiple-bond correlation (GSQMBC) experiment], the quinoline-N (N-5) was observed in the usual range at d 135.6 ppm, whereas the indole-N (N-11), which has is iminelike in nature, was found to be signicantly deshielded (d 248.1 ppm).
Density functional theory (DFT) calculations of the chemical shielding constants were also performed, allowing a detailed investigation of the effects of protonation and solvation. The calculations were able to reproduce the main experimental trends observed upon protonation, and it was observed that the inclusion of solvent effects in the computations improved the agreement with the experimental 13 C NMR data. 1 H NMR data was also recorded in order to determine that the pK a of the natural product is 9.8. This result was obtained through a graphical estimation of the inection point and also by the use of the Henderson-Hasselbalch equation [eqn (1)], 30 which showed a linear dependence of the chemical shi on the pH of the sample: 31 The effect of cyclodextrin (CD) encapsulation on the photophysics of isocryptolepine, was studied using steady state and time-resolved uorescence spectroscopy. 32 In the excited state, it was observed that the natural product exists mainly in its zwitterionic form, exhibiting appreciable emission from the pp* state upon excitation at a specic wavelength.
Due to the presence of the hydrophobic nanocavities of CDs, the existence of zwitterions in the excited state allows a mutual interaction to form dimers, triggered through coulombic interactions. This was evidenced by treating the uorophores with CDs with different cavity spaces and employing steady state uorescence measurements. Moreover, the photophysical behavior of the heterocycle was found to be modulated by the nature of the cyclodextrin. In addition, trapped water molecules inside the bigger cavity of g-CD seemed to quench the uorescence of the zwitterions.
Isocryptolepine has been demonstrated to show antimicrobial, 33 anti-trypanosomal, 34 antifungal, 35 anti-inammatory, antimalarial (including b-haematin inhibition), 36 antitrypanosomal, antileishmanial and cytotoxic activities, 34,36c,37 as well as the ability to interact with DNA, 38 promote low density lipoprotein uptake in HepG2 cells, 39 and be useful as a treatment for intestinal disorders. Most of these properties are common to other natural indoloquinolines, which differing in potency and selectivity.
As a result of its prole, during the last 20 years, this natural product became a recurrent target for total synthesis as well as a useful scaffold for the preparation of analogs 40 and derivatives, 41 with the aim of improving its activity prole (mainly its potency and selectivity). Furthermore, its attractive structure turned its synthesis into a fertile eld for testing the scope and efficiency of new and imaginative C-C and C-N bond forming reactions. Detailed below are the different approaches that have been designed to synthesize isocryptolepine.

Total syntheses of isocryptolepine
Isocryptolepine is an angularly fused indoloquinoline. As observed in the case of other indoloquinolines, there has been some relevant synthetic work that focused on targeting isocryptolepine before it was found in nature, in 1996. However, most of the synthetic efforts toward the alkaloid have been carried out over the last 20 years, where the natural product has been totally synthesized over 25 times, by different groups around the world; furthermore, some of them can be credited with more than one synthesis.
We have previously classied the synthetic approaches toward the indoloquinoline alkaloids neocryptolepine (6) and quindoline (2) into three main groups, based on the chemical structure of the starting materials involved in the sequence, including: (a) benzenoids, (b) quinolines and (c) indoles. 42 Accordingly, except for the advances recorded before the isolation of isocryptolepine from C. sanguinolenta, the total syntheses of the natural product are classied here to t into these three categories. In addition, for the sake of clarity, the syntheses are ordered chronologically.
4a. Early synthetic studies before the isolation of isocryptolepine from a natural source The rst reported total synthesis of the structure of isocryptolepine was disclosed in 1950 by Kermack and Storey,43 who synthesized derivatives of the tetracycle 1 for biological testing, in an attempt to nd new antimalarials. These authors employed a modication of the Graebe-Ullmann carbazole synthesis for the preparation of the tetracyclic framework of their prospective compounds and also synthesized isocryptolepine via the N-methylation of 1.
The product was obtained as a violet uorescent compound, which exhibited increased uorescence when dissolved in concentrated sulfuric acid. Finally, the tetracycle was heated in a steam bath with excess methyl iodide in nitrobenzene, furnishing the methiodide of 7 in 72% yield. The base of the latter could be freed by treatment with aqueous ammonia, followed by drying of the resulting isocryptolepine monohydrate.
In the reaction mechanism (Scheme 2), the primary amino group of 13 is diazotized with HNO 2 to give the diazonium salt intermediate A, which undergoes an intramolecular cyclization to the protonated benzotriazole intermediate B. Aer deprotonation, benzotriazole 14 loses nitrogen to afford the diradical species C, which in its conformation D undergoes a radical rearrangement to the carbene intermediate E. Then, cyclization takes place to afford intermediate F, which further aromatizes to 1, via a [1,3] proton shi.
This contribution complemented the earlier ndings by Clemo and Perkin, 44 which can be regarded as a synthesis of an advanced intermediate of the natural product. These authors demonstrated that upon heating with aqueous sulfuric acid, the phenylhydrazone of 1,2,3,4-tetrahydroquinolin-4-one underwent a one-pot Fischer indolization and subsequent dehydrogenation to give 1. Employing the same approach but starting with the phenylhydrazone of N-methyl-1,2,3,4tetrahydroquinolin-4-one (obtained from N-methylaniline in 15% yield) Braunholtz and Mann also synthesized isocryptolepine in 1955 in an unspecied yield. 45 In 1965, Roussel et al. reported an alternative approach toward 1 (Scheme 3) by way of the 1,2,3,4-tetrahydroquinolin-4-one 16. 46 The ketone was best prepared in 68% yield by direct cyclization of b-N-phenylalanine (15, obtained in turn from aniline and methyl acrylate) with PPA at 130 C for 20 min. However, 16 could also be accessed in three steps and in substantially the same yield via the aza-Michael addition of aniline to methyl acrylate, followed by ester hydrolysis and cyclization.
The latter was converted into the tosylamide 17 and further indolized in 50% overall yield by rst heating it for 90 min at 80 C with phenylhydrazine in EtOH, to give the hydrazone 18, and then heating it for 10 min in AcOH, under H 2 SO 4 promotion. The cyclization stage was accompanied by both detosylation and dehydrogenation toward 1.  In 1968, García et al. disclosed an additional alternative process of synthesizing [3,2-c]indoloquinoline (1). 47 These authors prepared 3-(2-uorobenzoyl)indole (19) via the acylation of indolylmagnesium bromide with o-uorobenzoyl chloride (Scheme 4) and found that the subsequent treatment of the latter with ethylenediamine in reuxing pyridine gave the substituted aminobenzoylindole imine derivative 20 in 54% yield via the nucleophilic exchange of uorine for an amine. 48 Upon acid treatment, 20 underwent rearrangement to afford a mixture of the quinolone 21 and the indoloquinoline 1. However, heating the quinolone in diphenyl ether under reux gave the fused heterocycle 1 as the sole product, apparently in very low yield.
In 1993, the Alvarez-Builla group improved and simplied the original sequence of Kermack and Storey, 38a,49 by reporting a one-pot Graebe-Ullmann approach to the tetracyclic precursor 1 of the natural product, as a surrogate for the traditional Fischer indolization approach (Scheme 1). The process, which was later employed by Murray et al. for the preparation of analogs for biological evaluation as antimalarials, 36d could be carried out in an open vessel using a domestic microwave oven.
The strategy involved two irradiation stages; rst, a solventless mixture of 11 and 15 was irradiated at 160 W to obtain the benzotriazole derivative 14 in 90% yield; then, without purication of the benzotriazole, a second irradiation process was performed at 160 W in the presence of pyrophosphoric acid to produce the tetracycle 1 in 60% yield aer 4-6 min. Interestingly, it was observed that the attained yield under these conditions was similar to that obtained by applying conventional heating. It was also concluded that irradiation of a solventless mixture of the pure reactants was more efficient than using their supported forms, over silica gel or montmorillonite.

4b. Syntheses from benzenoids
Synthesis by Novikov et al.. The rst total synthesis of isocryptolepine aer its isolation as a natural product was carried out starting from simple benzenoid compounds, reported in 1996 by the Novikov group (Scheme 5). 50 The synthetic pathway, which was reported in few details, followed the indolization strategy originally devised by Clemo and Perkin. 44 The nal product was obtained from N-tosylaniline (22) via a six-step sequence in an overall yield of 19.2%. To that end, the authors performed the N-alkylation of 22 with b-chloropropionic acid 51 to obtain the sulfonamide 23 in 69% yield and cyclized the latter by heating at 130 C with polyphosphoric acid, 52 to access the required intermediate carbonyl derivative 17 in 96% yield.
In turn, the 1,2,3,4-tetrahydroquinolin-4-one 17 was exposed to phenylhydrazine under AcOH catalysis, furnishing 87% yield of the intermediate hydrazone 18. Subsequently, this compound was subjected to indolization with H 2 SO 4 in acetic acid at 100 C, to give 1 in 56% yield. Exposure of the tetracycle 1 to MeI afforded the mono-methiodide 24 in 64% yield. Final basic treatment to free the base gave isocryptolepine (7) in 93% yield. Interestingly, in the presence of K 2 CO 3 , the bis-N-methylated isocryptolepinium derivative 26 was obtained in 82% yield. More recently, 26 was accessed by Volvoikar and Tilve in 75% yield from 25, employing a cross-dehydrogenative coupling reaction with TBHP and I 2 . 53 The mechanism of the transformation of the hydrazone intermediate 18 into the tetracycle 1 is depicted in Scheme 6. It is proposed that the protonation of 18 to afford A would give B aer tautomerization. In turn, the latter could become protonated (C) and rearrange to D followed by rearomatization to give the anilino-iminium ion E. Nucleophilic attack of the aniline of the iminium moiety would afford intermediate F, which could then provide 1 aer losing ammonia and then toluenesulnic acid, driven by the possibility of aromatization.
Synthesis by Molina et al.. The Molina group, which has been active in the synthesis of indoloquinolines and related heterocycles through the use of intramolecular aza-Wittig reactions, presented in 1999 their total syntheses of both cryptotackieine (neocryptolepine) and isocryptolepine from a common key intermediate, via a selective indolization process. 54 In their rather long synthetic sequence, the phosphonium salt 27 was condensed with the azidobenzaldehyde 28 employing K 2 CO 3 and dibenzo-18-crown-6, to give the stilbene This journal is © The Royal Society of Chemistry 2020 RSC Adv., 2020, 10, 18978-19002 | 18983 derivative 29 in 85% yield as a 4 : 1 mixture of Z : E isomers (Scheme 7). Next, the Staudinger reaction of the azide 29 with n-Bu 3 P provided the iminophosphorane 30 which was hydrolyzed to the aniline 31 in 84% overall yield, as a mixture of isomers (Z : E ¼ 7 : 1). Subsequently, the catalytic isomerization of the double bond with the thiophenol/AIBN reagent system afforded E-aminostilbene 31 in 92% yield. This compound contains the nitrogen atoms required to install both heterocyclic rings.
Treatment of E-31 with triphosgene and further microwavepromoted cyclization of the resulting isocyanate 32 furnished the quinolin-2-one derivative 33 in 80% overall yield. 55 The latter was next transformed into the common intermediate 36 by means of a three-step sequence involving N-methylation to give 34 (82% yield) followed by Pd/C-mediated catalytic hydrogenation to afford 35 in 91% yield and nal one-pot treatment of the amine with HNO 2 , and subsequent reaction of the resulting diazo intermediate with NaN 3 to provide the azide derivative 36 in 85% yield.
The indolization of the azide 36 toward the framework of isocryptolepine was performed by reuxing the azide in orthoxylene, which afforded 37 (isocryptolepinone) in 82% yield. Interestingly, 37 was previously prepared from N-methyl-4azido-3-phenylquinolin-2-one, by reuxing in dimethylformamide (DMF) for 2 h (72% yield) through quite a similar nitrenebased reaction, and in 61% yield via irradiation with a low pressure mercury lamp at room temperature for 4 h. 56 More recently, the Sagar group reported the synthesis of the lactam 37 in 68% yield via the reductive cyclization of 34 under MoO 2 -Cl 2 (DMF) 2 catalysis. 57 Final reduction of the carbonyl moiety with Red-Al in toluene at reux gave isocryptolepine (7) in 90% yield. Interestingly, treatment of 36 with the highly reactive Me 3 P gave an intermediate iminophosphorane, which upon microwave irradiation in nitrobenzene at 150-180 C for 30 min furnished cryptotackieine (6) in 40% yield via an aza-Wittig type reaction. 58 Synthesis by Kundu et al.. In 2009, the Kundu group reported an alternative synthesis of isocryptolepine.
Unlike most of the previous strategies, which relied predominantly on the formation of the indole by ring closure to culminate the synthesis, their approach was characterized by the formation of the quinoline ring in the nal step. 59 The sequence was based on Kundu's modied version of the Pictet-Spengler cyclization. 60 To nd the correct conditions, they rst put in place a Fischer indole synthesis between phenylhydrazine (38) and 2-methylaminoacetophenone (39) 61 to afford the intermediate 40 (Scheme 8). 62 The latter was then subjected to a Pictet-Spengler reaction with paraformaldehyde in the presence of TFA, to directly furnish the indoloquinoline 44. Presumably 44 was formed through the intermediacy of imine 43. In turn, 44 underwent spontaneous aerobic oxidation to afford 1 in 86% yield. 63 Then, the tetracycle was subjected to selective N-methylation, by treatment with MeI in reuxing toluene to furnish isocryptolepine.
Interestingly, when exposed to Hendrickson's reagent (Tf 2 O-PPh 3 O), the N-formyl derivative 41 gave 1 in 75% yield, through a regioselective 6-endo-cyclization. 64 The precursor 41 was elaborated efficiently via a gold(III)-catalyzed 5-endo-dig cyclization. 65 On the other hand, a regioselective copper-catalyzed onepot cascade reaction of bromoarene 42 with formaldehyde and aqueous ammonia in DMSO has been developed, which affords 1 in 32% yield. 66 First synthesis by Kraus et al.. Kraus and coworkers developed a exible and high yield synthesis of indoles based on the electrocyclic ring closure of the anion of a Schiff base derived from the reaction of the commercial 2-aminoarylbenzylphosphonium salt 45 with an aromatic aldehyde. 67 In 2010, they applied their strategy to develop a formal total synthesis of isocryptolepine, starting with 2-nitrobenzaldehyde (46). 68 Thus, the required imine 47 was synthesized by a reaction between 45 and 46 under AcOH catalysis, employing microwave irradiation at 80 C on a methanolic solution of the reactants (Scheme 9). The reaction of the as-formed nitro-imine 47 with K t BuO in THF at 0 C gave the indole 48 in 72% overall yield, presumably through the anionic species A, which may lose PPh 3 aer undergoing cyclization to the intermediate B, which in turn undergoes a nal H-shi and aromatization.
Reduction of the nitro group with elemental iron (Fe 0 ) and HCl in EtOH to aniline 40 and further formylation of the indole moiety with paraformaldehyde and TFA with subsequent in situ cyclization completed the tetracycle 1 in 82% yield. Finally, according to Agarwal et al., 59 the methylation of 1 with MeI in toluene gave 7 in 91% yield.
Second synthesis by Kraus et al.. In 2010, the same group reported another application of the commercial 2-aminoarylbenzylphosphonium salt 45 as an alternative formal total synthesis of isocryptolepine. 69 To that end (Scheme 10), they started with the known carboxylic acid 49, easily available from isatin in 92% yield, 70 and prepared the acid chloride 50 by reaction with thionyl chloride or oxalyl chloride.
The product was coupled with the phosphonium bromide 45 and the resulting amide 51 was treated with potassium tertbutoxide at room temperature, where it underwent an intramolecular Wittig reaction to afford lactam 52 in 62% overall yield from 49.
The N-methylation of 52 with methyl iodide in the presence of K 2 CO 3 in DMF gave 36 in 98% yield. This lactam is a key intermediate in the total synthesis of isocryptolepine by Molina. 54b The latter group invested in nine steps to arrive at the intermediate 36, in 22% overall yield, whereas this shortcut advantageously accomplished the same purpose in 60% yield over 4 steps in only two steps.  (55). This was performed via a coupling reaction between 53 and the diazo-derivative of 2-nitroaniline (54), followed by sequential reductive deoxygenation of the nitro and formyl moieties. 71 The authors demonstrated that compound 55 can be further transformed into 3-(2-acylvinyl)-2-(hetero) arylindole (56) through an unusual furan-to-indole recyclization process (Scheme 11). 72 The substrate scope of this imaginative approach to the synthesis of more complex indole derivatives was demonstrated by the authors through the total synthesis of isocryptolepine (7) and the preparation of differently decorated analogs of the natural product. 73 The reaction of 55 with 2-nitrobenzaldehyde afforded the expected indole derivative 56, but in comparative lower yield (58%) than for other aldehydes. This was explained as being the result of a combination of unfavorable steric and electronic effects of the ortho-nitro moiety, which affected the iminium ion attack on the furan ring.
A possible reaction mechanism includes a rst step, where the Schiff base A is formed by the condensation of 55 with the aldehyde. Then, protonation of this intermediate (B) should drive the ring opening of the furan motif and afford the oxaazaspirocyclic intermediate C, which aer undergoing ring opening of the dihydrofuran moiety triggered by aromatization of the nitrogen ring, affords the 2-substituted indole 56.
In the next stage, the reduction of the nitro group, various reagent systems were studied (Zn/AcOH, Zn/NaOH, RANEY® nickel, Fe/AcOH), with the nding that Fe in AcOH directly affords the indoloquinoline 1 in 86% yield. The formation of 1 could result from the reduction of the nitro group to the corresponding aniline 57, followed by an intramolecular Michael addition to give intermediate D, 74 and aromatization of the pyridine ring of the latter via the rather unusual elimination of acetone. 75 For the N-methylation stage, the best result (86% yield) was obtained using the Kermack-Storey procedure, employing MeI in nitrobenzene. 43 Synthesis by Bogányi  catalyzed reactions, namely a regioselective Buchwald-Hartwig amination and a subsequent Heck-type biaryl coupling.
Their contribution included the preparation of the tetracycle 1, which constitutes a formal total synthesis of isocryptolepine. 76 For this purpose, the authors specically prepared the 3,4-dihaloquinoline 63 (Scheme 12).
The key 1H-quinolin-4-one intermediate 60 was obtained in 77% yield, via the condensation of the aniline 58 with Meldrum's acid in the presence of trimethyl orthoformate to afford 59, followed by cyclative thermolysis of the latter in diphenyl ether. 77 The direct halogenation of 60 with bromine in AcOH 78 gave the expected 3-bromo-1H-quinolin-4-one 61 in 99% yield, whilst treatment of the latter with POCl 3 afforded 3-bromo-4chloroquinoline (62) in93% yield. Final installation of an iodide motif was accomplished via a Finkelstein-like displacement of the chloride with NaI in MeCN, affording the desired heterocycle 63 in 55% yield. 79 Next, the dihaloquinoline 63 was coupled with aniline in toluene, employing palladium catalysis, with Xantphos as a ligand and Cs 2 CO 3 as a base, under microwave irradiation at 120 C for 90 min, to selectively afford 64 in 95% yield. Final cyclization of 64 to the indoloquinoline motif 1 was performed in 64% yield, through an intramolecular Heck-type reaction in DMA, under microwave irradiation (1 h at 150 C), using PdCl 2 (PPh 3 ) 2 as a catalyst and NaOAc as a base. 80 The Maes group prepared analogous intermediates through a different synthetic sequence and developed several syntheses of various indoloquinolines. An interesting advantage of the Kámán synthesis over the previous reports by Maes et al. on the tandem palladium-catalyzed approaches to indoloquinolines is that in the latter cases, small amounts of the undesired regioisomer were detected aer the ring closure. 10a,80 Synthesis by Lu, Xu et al.. Organic electrosynthesis, which employs electrons as reagents, 81 has been demonstrated to be a versatile and environmentally friendly tool for the synthesis of structurally complex compounds. 82 Lu, Xu and coworkers developed an electrochemical method for generating amidyl radicals and demonstrated that these species could participate in cascade cyclization reactions 83 to afford indolines. 84 Their approach was applied in 2016 to the synthesis of highly functionalized indoles, including isocryptolepine, via C-H/N-H functionalization of (hetero)-arylamines with tethered alkynes. 85 The synthesis (Scheme 13) commenced with a Sonogashira cross-coupling between N-methyl-2-iodoaniline (65) and the acetylenic acetal 66 in Et 3 N-THF at room temperature. The subsequent reaction of the resulting intermediate, obtained in 81% yield, with phenylisocyanate in toluene, gave the urea 67 in 78% yield.
Compound 67 became a suitable substrate for the electrolysis, which was carried out at a constant current of 10 mA on the gram-scale, in the presence of ferrocene as a redox relay reagent. The electrolyte was a mixture of sodium carbonate and tetrabutylammonium tetrauorborate in THF-MeOH. A platinum plate was used as a cathode and reticulated vitreous carbon served as an anode.
This process afforded the tetracyclic acetalic compound 68, which aer its subsequent acid-promoted hydrolysis resulted in the formation of the formyl-substituted indole 69 in 63% overall yield. Compound 69 was readily transformed into isocryptolepine (7) in one step and 91% yield, via the basepromoted hydrolysis of the urea motif, which freed the Nmethylaniline moiety, enabling its spontaneous intramolecular condensation with the free formyl group. Among the advantages of this approach are the facts that it proceeds through the liberation of H 2 and does not require the use of noble-metal catalysts and external oxidants for the cyclization stages. This journal is © The Royal Society of Chemistry 2020 RSC Adv., 2020, 10, 18978-19002 | 18987 A plausible mechanism for the electrochemical formation of the product was proposed based on previous studies. 84 Accordingly, the anodic oxidation of [Cp 2 Fe] to [Cp 2 Fe] + takes place with the concomitant cathodic reduction of MeOH to form the methoxide anion and release H 2 . Then, the base deprotonates the amino group of the substrate 67 to furnish the anion A, which is a much better electron donor than its neutral precursor.
Therefore, single-electron transfer (SET) takes place between intermediate A and [Cp 2 Fe] + to afford the electron-decient nitrogen-centered radical B 86 and regenerate Cp 2 Fe. 87 It was suggested that the SET is so efficient, that it competes well with the cathodic reduction of [Cp 2 Fe] + , enabling the electrolysis to be executed in an undivided cell. Next, the intermediate B participates in a rare 6-exo-dig cyclization 83 to give the vinyl radical C, which then undergoes a second cyclization with the aryl ring, to afford the delocalized tetracyclic radical D. Finally, the rearomatization of the latter by electron and proton eliminations generates the product 68.
Synthesis by Aksenov et al.. The 2009 synthesis of isocryptolepine by Kundu and co-workers comprised four steps, which involved a Fischer indolization to build a suitable 2-arylindole intermediate and the installation of the quinoline ring via an oxidative Pictet-Spengler type reaction of an N-arylmethanimine, in $50% overall yield. Shortly aer, Aksenov and coworkers developed a sequential three-component heteroannulation reaction toward 3-aryl-2-quinolones that proceeds via a cascade transformation and involves a Fisher indole synthesis with PPA as the reaction medium. The authors observed that the P 2 O 5 -water ratio can be accurately tuned in the promoter to provide optimal yields of the cyclized product, and proposed to use their development for a simplied and improved total synthesis of isocryptolepine, under the original retrosynthetic guidelines of Kundu. 88 In that direction, in 2017 Aksenov et al. conjectured that PPA could promote a one pot synthesis of the intermediate hydrazone 70 required 89 for Fischer indolization (Scheme 14), as well as drive to completion the formation of the indole moiety (71), and also participate in the subsequent intramolecular Vilsmeier reaction between an aromatic amine and a carboxylic acid, for the nal cyclization leading to isocryptolepine (7). 90 However, PPA failed to promote the synthesis of the hydrazone between ortho-aminoacetophenone (39) and phenylhydrazine (38), favoring self-condensation of the ketone, and this step had to be performed in EtOH under formic acid catalysis 91 or even better, under solventless conditions, for 10 min at 120 C. Furthermore, despite PPA was able to promote the Vilsmeier cyclization with various carboxylic acids, but formic acid proved to be unstable to the reaction conditions, decomposing into CO and CO 2 and furnishing 7 in only 25% yield. 92 Therefore, a formic acid surrogate was sought and triazine was proposed to fulll this place. The authors found that once hydrazone 70 was formed, it could be treated with PPA to afford indole 71, and shortly aer with triazine (72) to produce isocryptolepine as the sole reaction product in an optimized 82% yield, using a convenient metal-free process.
The proposed reaction mechanism of the transformation involving triazine as a formic acid equivalent comprises the addition of the triazine (72) to the Fischer indolization product 71 to produce species A. Next, ring opening of the dihydrotriazine and subsequent intramolecular electrophilic attack of the resulting linear species B at C3 of the indole furnishes the six-membered heterocyclic ring (C). Finally, proton transfer, followed by an elimination (D) anchimerically-assisted by the quinoline nitrogen, affords isocryptolepine. An alternate deprotonation/elimination sequence leading to 7 has also been proposed. In addition, the same group devised a second generation PPA-mediated one-pot three-component synthesis of the indoloquinoline scaffold employing nitroalkanes. The use of MeNO 2 in place of the triazine 72 furnished 7. 93 Interestingly, the use of dimethyl sulfoxide (DMSO) as a onecarbon atom synthon in a similar transformation leading to the unsubstituted indoloquinoline skeleton in 69% yield was recently reported. 94 In this case, it was proposed that two mechanistic pathways are possible. In the rst case, at high temperature, DMSO may generate methyl radicals (Mec), which can react with O 2 to produce a peroxy radical and then CH 2 O. 95 In turn, this could result in imine formation and a subsequent electrophilic aromatic substitution to produce the observed product. It is also possible that DMSO could decompose at high temperature, and produce CH 2 O directly. 96

4c. Syntheses from quinolines
The total syntheses of isocryptolepine, which employs quinolines as starting materials, comprise synthetic efforts where haloquinolines or 1,2,3,4-tetrahydroquinolin-4-one derivatives were employed. They complement other accomplishments, such as that of Bogányi and Kámán, 76 where quinolines were prepared as intermediates from structurally simple benzenoids.
Synthesis by Tímari et al.. The rst total synthesis of isocryptolepine starting from quinolines was reported in 1997 by the Tímari group (Scheme 15). 97 The sequence resorted to a Suzuki cross-coupling 98 reaction between N-pivaloylamino phenylboronic acid (74) 99 and 3-bromoquinoline (73) to afford the biaryl derivative 75 in 90% yield. Next, the acid-catalyzed removal of the protecting group furnished 93% yield of the free amine 76, which was subjected to a one-pot diazotization and subsequent reaction with NaN 3 to deliver the azide derivative 77 in 80% overall yield. 100 The thermal cyclization of 77 was executed in reuxing dichlorobenzene, and proceeded in 70% yield to exclusively furnish the required tetracycle 1 in 75% yield, 101 by way of a singlet nitrene intermediate that inserts into the C4-H bond. The reaction of 1 with Me 2 SO 4 in MeCN resulted in the regioselective alkylation of the pyridine nitrogen, affording isocryptolepine (7) in 93% yield aer freeing the base by treatment with potassium carbonate.
An alternate pathway for accessing 1 in one pot and 46% yield from 78 was recently disclosed by Ess, Kürti and coworkers, through the electrophilic amination of the quinoline C4-H bond with PhMgBr. 102 On the other hand, the Sydnes group disclosed the preparation of tetracycle 1 from the aniline derivative 76 in 73% yield, employing a palladium-catalyzed C-H activation/C-N bond formation strategy. 103 Syntheses by Maes et al.. In 2003, the Maes group 104 developed a three-step synthesis of isocryptolepine (Scheme 16) based on the combination of two selective Pd-catalyzed transformations, namely a Buchwald-Hartwig reaction 105 and an intramolecular direct arylation. 106 Thus, the arylamination of 4chloroquinoline 107 (11) and 79 with Pd(OAc) 2 and K 2 CO 3 as a base, in reuxing dioxane, afforded the amine 81 in 60% yield when BINAP was employed as the metal ligand.
However, the use of Xantphos as a ligand improved the yield to 81% when Cs 2 CO 3 was employed as a base. 108 In order to execute the required direct arylation of 81, a nucleophilic palladium catalyst system better than Pd/BINAP was deemed necessary. 109 Therefore, trialkylphosphine ligands, which are more electron rich than their triaryl counterparts, were examined, with the nding that the cyclization could be carried out to afford a 95% yield of 1 when tributylphosphine was used, 110 in the presence of potassium phosphate.
The direct arylation leading to 1 was considered a notable example of the intramolecular Pd-catalyzed arylation of an electron-decient heteroaromatic. Examples of these reactions are scarcer in the literature compared to those of electron-rich heteroarenes. 111 Final, methylation of 1 with MeI in DMF, followed by treatment with Na 2 CO 3 to release the base, afforded isocryptolepine (7) in 57% yield.
The synthetic strategy was further improved by these authors in 2008, 112 with the development of an auto-tandem palladiumcatalyzed consecutive C-N and C-C bond forming process, which enabled the coupling of 4-chloroquinoline (11) and 2chloroaniline (79), somehow reminiscent of the auto-tandem syntheses of other carbazole systems. 106c,113 Employing the catalytic system previously used for the direct arylation [Pd 2 -Cl 2 (dba) 3 , P( t Bu) 3 ], with K 3 PO 4 as a mild base for improved compatibility, the tetracyclic compound 1 was obtained in 82% yield. It was also observed that despite K 3 PO 4 not dissolving completely in the reaction medium, the addition of an excess of reagent can speed-up the reaction.
Syntheses by Mohan et al.. The Mohan group achieved many advances in the synthesis of indoloquinolines, with two of their total syntheses of isocryptolepine employing quinoline derivatives as starting materials. In 2005 they reported the rst of these total syntheses, which was based on the Fischer indole cyclization of the 1,3-dicarbonyl derivative 82 (Scheme 17). 114 To that end, 82 was exposed to phenylhydrazine hydrochloride (38), in a 4 : 1 mixture of glacial AcOH and concentrated HCl, to give the indoloquinolinone 37 in 65% yield. The latter existed predominantly in the hydroxy form (83); therefore, the required aromatizative deoxygenation of 83 was performed as a two-step process, by reaction with POCl 3 (ref. 115) to easily form the intermediate chloro-derivative 84 (62% yield), which was hydrogenolyzed over 10% Pd/C to give isocryptolepine (7) in 70% yield.  The authors observed that the yield of product for the photocyclization in protic solvents was signicantly higher than that in benzene alone; hence, the reactions were carried out in a benzene : methanol : sulfuric acid (60 : 30 : 1, v/v/v) solution, to afford the tetracycle 1 in 78% yield. 10b The nal N-methylation of 1 was performed with methyl sulfate in acetonitrile, furnishing isocryptolepine (7) in 83% yield aer freeing the base with K 2 CO 3 . Furthermore, a similar approach culminating with the photoannulation of a 3-iodoquinoline derivative was employed by this group for the synthesis of a 6-methyl derivative of isocryptolepine. 116 In 2006, the same group reported an efficient three-step heteroatom directed photoannulation approach toward the natural product (Scheme 18). 10b In their synthetic sequence, 4chloroquinoline (1) was thermally aminated at 200 C in 74% yield with 2-chloroaniline (79), 117 120 The transformation involves the cyclocondensation toward the tetracycle 86 and its further dehydrogenation in situ 10b,89a,121 to afford the nal product. The authors tested different alternative acidic promoters, discovering that heating with a 1.1 equiv. TsOH at 100 C gave the best result (83% yield).

4d. Syntheses from indoles
The approaches to the total synthesis of isocryptolepine from indoles are characterized by the use of N-protected and/or 3substituted indoles as starting materials. Although certain syntheses have used an unprotected indole derivative and others have started with 2-substituted indoles, they are fewer in number. Electron withdrawing groups (benzenesulfonyl, Boc) do not always meet C-C or C-N bond formation requirements; in such cases protective groups such as SEM, MOM and benzyl ether have been used.
Synthesis by Joule et al.. The rst indole-based total synthesis of isocryptolepine was reported in 1998 by the Joule group, as a different approach toward the natural product (Scheme 20). The sequence employed the indole-stannane derivative 87 as a starting material and resorted to an intramolecular Vilsmeier reaction to build the 3-aminoalkylidene-3H-indole motif of the natural product. 122 Therefore, the 2-tributylstannyl indole 87 was N-protected as the SEM derivative and coupled with 2-iodonitrobenzene under palladium catalysis, to give the 2-(ortho-nitrophenyl)indole 88 in 98% yield. The catalytic hydrogenation of the nitro group (98% yield), followed by N-formylation with acetic formic anhydride (AFA) 123 and subsequent N-methylation of the resulting amine with methyl iodide, gave 89 in 95% overall yield.
The latter was subjected to an intramolecular Vilsmeier ring closure with sulfuric acid in ethanol, to afford 7 in 50% yield. Interestingly, protection of the starting indole as the N-phenylsulfonyl derivative produced complex mixtures, presumably due to the deactivating effect of the sulfonyl moiety toward the nal electrophilic attack.
Synthesis by Mohan et al.. The Mohan group developed photochemical approaches to the synthesis of several heteroannelated acridines, 124 including indoloacridines. 125 In 2002, they extended their strategy to the total synthesis of isocryptolepine. 89a The synthetic sequence employed simple and readily available starting materials, and involved the reaction of indole-3-carbaldehyde (90) with aniline (58) in glacial AcOH to afford 85% yield of the Schiff base 91, which exhibits a 1,3,5hexatriene motif (Scheme 21). Then, 91 was dissolved in benzene-methanol and exposed to short wavelength UV-light (254 nm) in the presence of iodine, to give the tetracyclic system 1. 126a It is worth noting that a visible-light-mediated aerobic tandem dehydrogenative Povarov/aromatization reaction with iodine 126b for the synthesis of isocryptolepines has been recently reported and the use of indole-3-carbaldehyde (90) as a precursor for the synthesis of bioactive alkaloids has been recently reviewed. 127 Mechanistically, 91 rst underwent photo-isomerization of the E-isomer to its Z-congener; then, the excited S1 state of the Z-isomer underwent an orbital symmetry-controlled conrotatory ring closure to the tetracyclic intermediate A.
These types of dihydro intermediates are sensitive to oxygen; 121 therefore, A underwent aromatization by way of the loss of the allylic hydrogens to form 1 in 67% yield, in the presence of iodine which acted as a mild oxidizing agent.
The nal regioselective methylation of 1 with Me 2 SO 4 in MeCN, in the presence of K 2 CO 3 , gave isocryptolepine in 83% yield. This total synthesis was complemented in 2006 with a three step photoannulation approach, which started from a quinoline derivative. 10b Synthesis by Miki et al.. The Miki group observed that indole-2,3-dicarboxylic anhydrides are useful synthons to access complex natural products. 128 In 2007, they devised an intramolecular decarboxylative Heck-type reaction for the new total synthesis of isocryptolepine, employing this type of heterocycle (Scheme 22). 129 In their approach, the anhydride moiety of the benzenesulfonyl derivative 92 was selectively opened upon exposure to N-methyl-2-iodoaniline (93) in MeCN at room temperature, to afford amide 94 in 77% yield.
Next, a projected decarboxylative Heck-type cyclization was executed following the protocol of Myers, by treatment of 94 with 20 mol% Pd(TFA) 2 and Ag 2 CO 3 in a mixture of 5% DMSO and DMF at 50 C for 48 h, 130 to give lactam 95 in 50% yield. Interestingly, a similar Heck cyclization was reported earlier by the Mérour group. 131 In that process, lactam 95 was desulfonylated in 80% yield, by treatment with TBAF in THF at room temperature for 6 h, 54 and the resulting tetracycle 37 was further reductively deoxygenated to isocryptolepine in 98% yield, by treatment with LiAlH 4 in hot dioxane.
Synthesis by Hingane and Kusurkar. In 2011, Hingane and Kusurkar reported an imaginative 6p-electrocyclization approach toward isocryptolepine, starting from N-phenylsulfonyl indole (96). 132 In their synthetic sequence, 96 was metallated with LDA and reacted with cyclohexanone to furnish the alcohol 97 (Scheme 23). Next, the hydrolytic N-desulfonylation of 97 was executed by treatment with TBAF in THF under reux for 2 h, and took place with the concomitant dehydration of the tertiary alcohol moiety to afford 98 in 84% yield. 133 The subsequent Vilsmeier-Haack formylation of 98 gave 99 in 88% yield, while attempted oximation of the latter with hydroxylamine in reuxing dioxane surprisingly furnished the tetracycle 101, presumably as a result of the initial formation of oxime 100 followed by a thermal intramolecular 6pelectrocyclization/elimination process. Therefore, the sequence continued with the dehydrogenation of 101 using Pd/ C in ortho-dichlorobenzene, which afforded 1 in good yield. Finally, treatment of 1 with methyl iodide to afford the methiodide and sodium carbonate to free the base, gave 7 in 37% overall yield.
Synthesis by Hibino et al.. In 2012, the Hibino group reported the total synthesis of isocryptolepine (7) via a microwaveassisted tandem Curtius rearrangement-electrocyclization of a 2-aza 6p-electron system (Scheme 24). 134 Following the procedure of Beaumont, these authors subjected the 2-iodobenzoate 102 and the indole-2-boronic acid 103 to a Suzuki-Miyaura cross-coupling reaction and obtained 104 in 99% yield. Since Boc proved to be an unsuitable protecting group for the advanced stages of the synthesis, 104 was exposed to TFA, resulting in the deprotection of the N-Boc group in 94% yield (105). 135 Aer trying different protecting groups, the authors selected the MOM group, which has minimum steric demand, to pursue the synthesis.
Hence, treatment of 105 with MOMCl in the presence of NaH gave the N-MOM-indolylbenzoate 106 in 91% yield and subsequent basic hydrolysis of the ester moiety with 1 M LiOH furnished the carboxylic acid 107 in 98% yield. The microwaveassisted Curtius rearrangement of the latter with DPPA in toluene at 100 C quickly provided tetracycle 109, resulting from a subsequent one-pot thermal 6p-azaelectrocyclic reaction, in an optimized 99% yield, without the isolation of the isocyanate intermediate 108.
The deoxygenation of the lactam, required to complete the total synthesis, was achieved by treatment of 109 with triic anhydride and pyridine, which afforded triate 110 in 83% yield. Next, the subsequent reductive cleavage of the latter with Et 3 SiH under Pd catalysis, according to the procedure of Katsuki, 136  This journal is © The Royal Society of Chemistry 2020 RSC Adv., 2020, 10, 18978-19002 | 18993 of azides with electrophiles under acidic conditions gives iminium ions, which can be trapped by alkenes and alkynes. 138 Speculating that the iminium ion could react with an aromatic nucleophile through an electrophilic aromatic substitution to afford a N-arylmethyl arene, in 2012 the Tummatorn group devised the four-step total synthesis of isocryptolepine (Scheme 25). 139 In their synthetic sequence, benzyl azide (112) and N-phenylsulfonyl indole (113) were exposed to TfOH in CH 2 Cl 2 to afford the tetracyclic compound 114 in 94% yield. Presumably, this reaction involved two sequential steps, namely the nucleophilic addition to the iminium ion followed by the intramolecular annulation of the resulting intermediate, resulting in a formal aza-[4 + 2]-cycloaddition.
Dehydrogenation of 114 with DDQ afforded 115 in 94% yield, which was desulfonylated in 95% yield with 2 M NaOH in reuxing MeOH to give the related indoloquinoline 1. The regioselective methylation of the latter with methyl sulfate nally furnished isocryptolepine in 78% yield. Functionalization of the precursor 114 has also been reported. 140 On the other hand, the basic synthetic sequence was employed as a key strategy toward the development of analogs of the natural product. 141 Synthesis by Lin et al.. Transition-metal catalyzed tandem C-C/C-N bond coupling reactions are an interesting strategy en route to nitrogenous compounds. 142 In 2010, Jiao's group reported a Pd(II)-catalyzed direct dehydrogenative annulation of indolecarboxamides with internal alkynes via C-H/N-H bond cleavage. Shortly aer, in 2014, Cui and coworkers developed a Rh(III)-catalyzed tandem coupling of N-methoxy-1H-indolecarboxamide and aryl boronic acids. 143 The Lin group studied the palladium-catalyzed coupling of N-methoxycarboxamides with iodobenzene (117). 144 They found that the reaction is capable of forming both C-C and C-N bonds in the presence of a suitable ligand, an oxidant and an indispensable silver source. PPh 3 proved to be one of the best ligands  to activate the metal, 145 whereas silver carbonate proved to be the most appropriate source of silver, also acting as an oxidant.
These authors applied the developed method to a new total synthesis of isocryptolepine (Scheme 26). Thus, the methoxyamide 116 was coupled with iodobenzene (117) under the optimized conditions to afford the tetracycle 118 in 67% yield. A mixed AcOH-DMA solvent was required in order to avoid decomposition of the starting material due to the considerable acidity of the solvent at the required high temperature. 146 Next, the latter was exposed to MeI and an excess of NaH at elevated temperature, in order to achieve simultaneous debenzylation and selective N-methylation with concomitant demethoxylation and afford the key intermediate 37 in 77% yield. 147 The nal deoxygenation of the lactam in 37 was accomplished by partial reduction with NaBH 4 in THF under the assistance of BF 3 $Et 2 O, followed by the in situ dehydration of the resulting lactol to afford 7 in 84% yield.
The reaction mechanism for the tandem C-C/C-N bond formation reaction (Scheme 27) involves the reaction of the starting material 116 with Pd(OAc) 2 to form a ve-membered palladacycle A, which undergoes oxidative addition of iodobenzene (117) to form the Pd IV species B. Next, B suffers a reductive elimination to give the arylated intermediate C, regenerating the catalyst.
Subsequent deprotonation of the amine in C and C-H activation affords the seven-membered palladacycle D, 148 which undergoes a reductive elimination to furnish 118 and a Pd 0 species. The latter is nally oxidized by Ag 2 CO 3 to regenerate the active Pd II species for the next catalytic cycle.
Synthesis by Mhaske et al.. Mhaske and coworkers reported the application of (NH 4 ) 2 S 2 O 8 -DMSO as an inexpensive, environmentally benign and safe reagent under mild neutral conditions, for the synthesis of imides, methylene bis-amides, diarylmethanes, and also for the Mannich reaction. 149 The usefulness of the developed protocol was demonstrated by a formal total synthesis of isocryptolepine (Scheme 28). 150 The synthetic sequence commenced with 3-benzoylindole, 151 which in the successful synthetic sequence was protected with a MOM group to afford 119, since electron withdrawing protecting groups (benzoyl, tosyl) failed during more advanced stages. In turn, 119 was converted to oxime 120 in 88% yield under conventional conditions, and the latter was subjected to the standard oxidation protocol to furnish the radical Beckmann rearrangement product, the amide 121, in moderate yield (51%).
A mechanism for this rearrangement was proposed. It was suggested that the (NH 4 ) 2 S 2 O 8 -DMSO system is a source of methylsulfanyl radicals (MeSc), which can attack the oxime moiety of 120 and afford the radical intermediate A. The latter can undergo a rearrangement to B and be further converted into the oxaziridine C, 152 with the loss of MeSH. In turn, the latter intermediate nally undergoes a rearrangement to afford amide 121. Radical intermediates have also been observed in the photochemically driven Beckmann rearrangement. 153 The as-formed intermediate 121 was exposed to Pd(OAc) 2 and Cu(OAc) 2 in AcOH at 120 C, which promoted an intramolecular double C-H activation, affording 109 in 66% yield. A similar C-C bond formation under UV light irradiation (254 nm) was reported earlier. 154 Compound 109 is a key intermediate in Hibino's synthesis of isocryptolepine; 134 therefore, this sequence constitutes a formal total synthesis of isocryptolepine. Additionally, compound 109 was deprotected with HCl in dioxane to obtain indoloquinolinone 122, a well-known bioactive scaffold, in 95% yield. 147a,155 Synthesis by Zhang et al.. The Zhang group developed several copper-mediated syntheses of natural products and in 2018 reported a selective cascade C-H/N-H annulation strategy toward the tetracyclic core of isocryptolepine, thus providing a new formal total synthesis of the natural product. 156 The bond forming approach is mediated by a 8-aminoquinoline N,Nbidentate directing group 157 and involves a reaction with a benzyne derivative under mild conditions.
The general transformation was optimized to use 0.35 equiv. of Cu(OAc) 2 and 1.2 equiv. CsF as a base, in the presence of 0.5 equiv. of TBAI, which enhances the generation of benzyne at 80 C in DMF : MeCN (1 : 1, v/v). The reaction took place under oxygen as a nal oxidant, for 12 h. The synthetic sequence leading to the isocryptolepine precursor involved the use of 5methoxy-8-aminoquinoline as a removable sacricial N,Nligand 158 and the Kobayashi benzyne precursor 124. 159 In the synthetic sequence, 124 was induced to react with the indole-3-carboxamide derivative 123 to afford 125 in 62% yield (Scheme 29), carrying a removable MOM group.
Then, the quinoline auxiliary was removed via a two-step protocol, including the selective O-demethylation of the quinolyl methyl ether with BBr 3 to free the phenol group, which was further oxidized with K 2 Cr 2 O 7 in AcOH : H 2 O (1 : 1, v/v), to afford lactam 109 in 65% yield, Hibino's intermediate toward isocryptolepine. 134 This reaction system avoids the use of sensitive and expensive noble metals and oxidants, and complements previous metal-based strategies.
A plausible mechanism for this C-H/N-H cascade annulation was presented, where a Cu(III)-mediated organometallic C-H activation takes place (Scheme 30). 160 Thus, 123 coordinates with Cu(OAc) 2 to generate an anionic complex A ligated by a N,Nbidentate directing group. 161 Then, the complex undergoes acetate-assisted intramolecular C-H concerted metalation/ deprotonation to afford the ve-membered ring complex B, which then undergoes carbocupration with the aryne generated from precursor 124 to furnish the intermediate C.
The nal reductive elimination of CuOAc C provides the desired annulation product (125), with the catalyst being regenerated for the next cycle in the presence of oxygen.
4e. Summary of the synthetic efforts toward isocryptolepine In general, the reported synthetic routes toward isocryptolepine required multistep linear sequences with the corresponding isolation and purication stages of the intermediate products. A comparison of them (Table 1), in terms of complexity and overall yield, is difficult and not straightforward, due to the variable nature and structural complexity of the starting materials. In addition, many syntheses of isocryptolepine are actually formal total syntheses, reaching only a previously known advanced intermediate toward the natural product. However, it is worth noting that several very short approaches, consisting of just one to three steps using commercially available materials, have been reported, and various approaches have allowed access to the natural product in more than 60% overall yield.

Conclusions and perspective
The indoloquinoline heterocyclic system is strongly associated with the West African plant Cryptolepis sanguinolenta, although other natural sources are known. Cryptosanguinolentine (isocryptolepine) has been isolated twice from this plant, widely used in traditional medicine.
Its unique framework and interesting pharmacological properties have meant that over the last 20 years isocryptolepine has become a test ground for new synthetic approaches toward C-C and C-N bond formation, with or without the use of precious metal catalysts. The natural product has been the target of over 25 synthetic efforts, where the tetracycle was built from benzenoids, quinolines and/or indoles via the stepwise construction of the heterocyclic moieties or through the successive assembly of the remaining heterocyclic rings.
The natural product has also gained recognition as an important and privileged scaffold in drug discovery due to the broad spectrum of its biological activities. Hence, many of the developed strategies have proved to be useful for the synthesis of more complex analogs and derivatives of the natural product, embodied with more potent and/or selective activity, mainly as cytotoxic and antimalarial agents. Therefore, it can be foreseen that the virtuous cycle, which involves new chemistries and novel structures for improved bioactive compounds, will keep going in the future, meaning that novel total syntheses of isocryptolepine will keep being disclosed.

Conflicts of interest
There are no conicts to declare