Supramolecular control of unidirectional rotary motion in a sterically overcrowded photoswitchable receptor

The control of molecular motion by external stimuli has attracted major interest in recent years. In order to achieve unidirectional rotational motion, intrinsically chiral light-driven molecular motors have been shown to be particularly versatile. We recently introduced a system in which unidirectional rotation is achieved in an achiral photoswitchable receptor upon binding of a chiral guest molecule. In order to gain detailed insight into the rotary steps, we present here a modi ﬁ ed design in which the steric crowding is increased such that interconversion between the helical isomers of the ( Z )-form of the receptor becomes very slow. DFT calculations support the increase in interconversion barrier. Furthermore, two diastereomeric complexes of the chiral guest bound to the ( Z )-receptor are distinguished in the 1 H NMR spectrum and the ratio between them slowly changes over time as a result of thermal helix inversion. On the other hand, no enrichment in one of the diastereomeric complexes is observed in the photochemical step. Our studies therefore unequivocally con ﬁ rm that net rotation, induced by the chiral guest, takes place. This study a ﬀ ords an improved understanding of these dynamic supramolecular systems and expands the pos-sibilities to control chirality transfer and motion at the molecular level, spurring developments of supramolecular recognition and information transfer at the nanoscale.


Introduction
For several decades now, chemists have strived to control motion at the molecular scale using appropriate chemical stimuli. [1][2][3][4][5][6][7][8] Among the different types of motion, achieving unidirectional rotations in a molecule has been a particular challenging task. [9][10][11][12][13][14][15][16][17][18][19] Our group 10,11 has developed light-driven rotary molecular motors based on sterically overcrowded alkenes, while Lehn 18 and Dube 19 introduced imines, and hemothioindigo motors, respectively. Light is considered particularly useful to power molecular motors, since it is noninvasive and it can be applied with high spatiotemporal control. Hence, light-driven molecular motors have been successfully applied in various functional molecular materials. 20,21 Nevertheless, novel designs and functions of molecular motors are still in high demand, especially with respect to (reversible) control over rotary motion using multiple stimuli, 22 allowing the future development of more sophisticated and complex molecular machinery.
In light of this, we recently reported a design in which unidirectional rotation in a stiff-stilbene based photoswitchable receptor, 23-26 decorated with thiourea groups, is directed supramolecularly via binding of a chiral phosphate guest (Scheme 1). 27 Here, the (E)-isomer of the photoswitchable anion receptor is converted to either the right-or left-handed helical form of its (Z)-isomer upon UV irradiation. In the presence of a chiral phosphate guest, the formation of one of these helical isomers is favoured and hence, the backward photochemical step takes place predominantly from this favoured isomer. The result is a net unidirectional rotation over the central double bond. While for previous light-driven molecular motors the direction of rotation was embedded in the ( pseudo-)asymmetry of the molecular structure, the direction of rotation in this case was determined by the chirality of the guest molecule and could therefore be changed at will. However, because of the fast interconversion between the possible diastereomeric complexes of the (Z)-receptor bound with the phosphate guest, it could not be determined if preferred enantiomer formation would already take place in the photo-chemical step, beside the thermal step, possibly impeding unidirectionality. Inspired by our work on overcrowded alkenebased molecular motors, in which increased steric hindrance slows down the thermal helix inversion process, [28][29][30] we now introduced a xylene (R = Me, Scheme 1) instead of a benzene motif in our stiff-stilbene derived bis-thiourea receptor. This modification allowed to monitor the thermal helix inversion at room temperature and to unequivocally establish that a net unidirectional rotation takes place. Furthermore, it shows that rotary speed modulation in this new type of supramolecularlydirected molecular motors is viable.

DFT calculations and synthesis
To assess the viability of our new design, we first conducted a series of DFT calculations at the SMD(DMSO)-ωB97X-D/def2-SVP(def2-TZVP) level of theory to predict the thermal isomerization barrier between the two helical forms of (Z)-1 (see the ESI for details †). To our delight, the predicted ΔG ‡ was ca. 25 kcal mol −1 at 298.15 K for the new xylene based system (see Fig. 1), which corresponds to a half-life of over one day, much larger than that previously calculated for the compound with the benzene motif (ΔG ‡ ca. 4 kcal mol −1 , t 1/2 < 1 ns). 27 The (E)-and (Z)-isomer of the bis-thiourea receptor were synthesized starting from the commercially available 1,4dibromo-2,5-dimethylbenzene 3 (Scheme 2). Indanone 5 was obtained in two steps following our previously reported protocol for a structurally related analog. 31 McMurry coupling was then employed to yield (E)-6 and (Z)-6 in a 10:1 ratio, and the isomers could be separated by precipitation due to the different solubility. 23 The low Z/E ratio in the McMurry coupling is ascribed to larger steric crowding in the (Z)configuration. 24,32 Subsequent Buchwald-Hartwig amination, followed by hydrolysis, afforded the bis-amine derivatives (E)-7 and (Z)-7, which were reacted with phenyl isothiocyanate to provide the corresponding bis-thioureas in good yield.

Guest binding properties
Next, binding studies with [Bu 4 N] + [(S)-2)] − were performed in DMSO-d 6 /0.5% H 2 O using 1 H NMR spectroscopy. Stepwise addition of [Bu 4 N] + [(S)-2)] − to a solution of (Z)-1 led to a significant downfield shift of the NH signals (Fig. 2a). Interestingly, these signals, together with the aromatic H a and methyl signals, splitted into two sets with virtually equal integration (Fig. 2a, for full spectrum see  . Hence, the first complex is 3.7 kcal mol −1 lower in energy than the latter (Fig. 3). In the (P)-(Z)-1 . (S)-2 complex, the formation of a stabilizing π-stacking interaction between the phenyl substituent of the guest and the receptor is allowed, most likely accounting for the higher stability. This is reflected in the NH⋯O bond to (E)-1 was also performed, but resulted in minor spectral changes, illustrative of very weak binding (see Fig. S2 in the ESI †). Additionally, the diastereomeric complexes were prepared in CD 2 Cl 2 and a similar change in ratio was observed ( Fig. S7 and S8 in the ESI †). However, equilibrium was reached faster (ca. 12 h) and the diastereomeric ratio (1 : 1.46) was higher than in DMSO. Furthermore, a change in CD absorption was observed with a freshly prepared solution of (Z)-1 in CH 2 Cl 2 with either (S)-2 or (R)-2 after several hours (Fig. S10 in the ESI †).

Photoswitching behavior
The photoinduced interconversion between the (E)-and (Z)form of the receptor was monitored by UV-vis and NMR spectroscopy in DMSO. Irradiation of (E)-1 with 312 nm light at room temperature resulted in a decrease of intensity of the absorption maximum at λ = 282 nm, and a new absorption band appeared at longer wavelength (Fig. 4a). These spectral changes are indicative of the formation of (Z)-1. 23 Starting from (Z)-1, the opposite transformation could be triggered by irradiation with 365 nm light, affording the opposite spectral changes (Fig. S11 in the ESI †). In both cases, irradiation was continued until no further changes were observed, meaning that the photostationary states (PSS) had been reached. The presence of a clear isosbestic point revealed the unimolecular nature of the photoisomerization process.
Irradiation with 312 nm light of a solution of (E)-1 in DMSO-d 6 /0.5%H 2 O led to the appearance of a new set of signals in the 1 H NMR spectrum, which could be ascribed to (Z)-1 (Fig. 4b). Subsequent irradiation with 365 nm light led to partial recovery of the initial 1 H NMR spectrum of (E)-1. The PSS 312 and PSS 365 ratios were determined by average integration of the urea and aromatic proton signals, affording   (Fig. 5a). Again, the PSS ratio was determined by the integration of aromatic proton signals and the ratio of 60 : 40 (E : Z) is similar to the ratio in absence of the anion. Very importantly, a 1 : 1 ratio of the diastereomeric complexes was observed immediately after irradiation. This result, combined with our finding that the thermal interconversion between

Conclusions
In summary, by synthesizing a photoaddressable stiff-stilbene receptor bearing a xylene core we have unequivocally established that supramolecularly induced unidirectional rotation takes place in the presence of a chiral phosphate guest. Binding of a chiral phosphate anion, e.g. (S)-2, to (Z)-1 led to the formation of two diastereomeric complexes in equal amount, which could be clearly distinguished in the 1 H NMR spectrum. Over time, however, the (M)-(Z)-1 . (S)-2 complex slowly converted by helix inversion at ambient temperature to (P)-(Z)-1 . (S)-2 and vice versa when (R)-2 was used. That is, with this design, the helix inversion process can be observed, while in our previous work, this process was too fast at room temperature. Furthermore, UV light irradiation of (E)-1 in the presence of the chiral phosphate guest resulted in equal formation of (P)-(Z)-1 and (M)-(Z)-1, illustrating that enrichment in one of the helical isomers takes place only in the thermally activated step, and not in the photochemical step. This work therefore provides direct evidence that the non-covalent chiral  phosphate binding induces unidirectional rotary motion since the chirality induction was observed in the ground state rather than in the excited state. These findings and new insights will further stimulate the use of supramolecular chirality transfer to control unidirectional rotary motion.

Conflicts of interest
There are no conflicts to declare.