The bi-metallic MOF-919 (Fe–Cu) nanozyme capable of bifunctional enzyme-mimicking catalytic activity

In this study, we report on a bi-metal organic framework, MOF-919 (Fe–Cu), capable of bifunctional-enzyme mimicking activity with oxidase- and peroxidase-like activities. The catalytic activities were examined by using o-phenylenediamine (OPD) as a chromogenic substrate to study oxidase- and peroxidase-like mimetics. Based on our findings, we developed a simple epinephrine colorimetric biosensor with a broad linear range (1–100 μM) and a low detection limit (0.298 μM). This approach provides evidence for transition metal-based pristine bi-metallic MOFs capable of reproducing both oxidase–peroxidase properties, which could be applied as new nanosensors.