Chemoselective formal β-functionalization of substituted aliphatic amides enabled by a facile stereoselective oxidation event

A facile dehydrogenation event enables the functionalization of aliphatic amides at different positions in a one-pot fashion.


Introduction
The carbonyl functional group remains one of the most important synthetic handles in organic chemistry. 1,2 While the a-carbon atom and the ipso-position of this ubiquitous structural motif can be modied in many ways, 2 the direct modication of the bposition is more elusive. With the advent of C-H functionalization, transformation of molecular moieties which are traditionally perceived as unreactive became feasible. [1][2][3] The past decades have witnessed an explosive wealth of accomplishments regarding this family of transformations. 3 Relying on the transient generation of internally chelated, cyclopalladated intermediates such as 2 (Scheme 1a), this mode of C-H functionalization has been effectively exploited particularly for b-methyl carbonyl derivatives. 4 The emerging eld of photoredox catalysis has also proven to be a powerful tool for the b-functionalization of cyclic ketones. 5 More conventional approaches make use of classical Michael addition strategies. However, the requisite a,b-unsaturated carbonyl compound is not always readily available. While the oxidative dehydrogenation of a-branched chloroenamines has been previously realized by Ghosez et al. 8b (though no E/Z selectivity was described), we have recently disclosed a selenium-mediated, one-pot a,b-dehydrogenation of amides (cf. -4 / 5, Scheme 1b) in moderate to good yields. More recently a stepwise dehydrogenation using a-TEMPO (tetramethylpiperidinyloxyl) amides as intermediates for the dehydrogenation of linear amides has been reported. 8c This approach is predicated on the electrophilic activation of carboxamides, a reactivity mode that enables transformations ranging from the venerable [2 + 2] cycloaddition 6a-d and ipso substitution 6e-g reactions all the way to the functionalization of the a-position via rearrangement and Umpolung chemistry. 7 However, the method depicted in Scheme 1b is limited to aunsubstituted amides. 8a In this manuscript, we report a stereoselective and mild a,b-dehydrogenation and how it enables not only the direct formal b-C(sp 3 )-H functionalization of simple amides under a metal-free regime, but also the functionalization of multiple carbon atoms in a,b and g-positions.

Preliminary results
During our studies on the Umpolung functionalization of amides, 7 we observed that a-branched amides deviate from Umpolung reactivity at the a-carbon. Instead, treatment of abranched amides with triuoromethanesulfonic anhydride (Tf 2 O), 2-iodo pyridine and pyridine N-oxide (PNO) resulted in clean and high-yielding a,b-dehydrogenation rather than the Umpolung reactivity we had previously observed. 9 Moreover, aaryl-substituted amides gave quantitative yield with the exclusive formation of one alkene-isomer. Importantly, careful analysis revealed that the dehydrogenation of a-aryl substituted amides gave exclusively the Z-olen (by NMR), whose structure was conrmed by NOESY-NMR spectroscopy (cf. Scheme 2a, 6a / 7a). On the other hand, a-branched dialkyl amides typied by 12 afforded an inseparable mixture of regio-and stereoisomers (Scheme 2b) with moderate selectivity. In particular, when the isopropyl-substituted, a-branched amide 14 was submitted to the reaction conditions we observed (Scheme 2c) equal amounts of the expected tetrasubstituted alkene 15a and the unexpected b,g-dehydrogenated amide 15b by NMR. The latter product is presumably formed by a Wagner-Meerwein rearrangement of the putative unstable carbocation 16 as shown (Scheme 2c). It is noteworthy that 14 reacted sluggishly.
The dehydrogenation reaction leads to very similar results regardless of whether 2-chloro-or 2-bromo pyridine is used instead of 2-iodo pyridine (a relevant piece of information for future developments, vide infra. See the ESI for further details †).
No reaction intermediates en route to dehydrogenation could be detected by NMR spectroscopy. We believe that the reaction proceeds by swi fragmentation of the enolonium species 19a to an a-carbocationic amide 20 (Scheme 3). 14 A rationale for the high Z/E selectivity is provided by the model shown in Scheme 3. Efficient mesomeric stabilization of the putative carbocationic intermediate mandates perpendicular arrangement of the adjacent aromatic moiety, resulting in restricted rotation of the arene substituent. 14 This exacerbates steric hindrance considerations, disfavoring a scenario where even a methyl group in the b-position is on the same side as the arene ring. As steric congestion increases during formation of the double bond, it is likely that elimination is greatly favoured from the thermodynamically favored conformer 20a.

One pot a,b-epoxidation
This surprisingly stereoselective and efficient result (quantitative conversion within minutes at room temperature) encouraged us to leverage in situ oxidation in order to achieve formal remote functionalization reactions. In particular, we were looking to draw on the high diastereoselectivity of the dehydrogenation event. In our hands, Prilezhaev oxidation using meta-chloroperbenzoic acid (commercial grade mCPBA) proceeded smoothly in quantitative yield at room temperature (Scheme 4). 10 When we investigated the possibility of a one pot procedure an unexpected hurdle was encountered, in that the  original procedure using 2-iodo pyridine for the dehydrogenation step was not compatible with the Prilezhaev oxidation, most likely due to the easily oxidized iodine atom. This issue was readily circumvented by using 2-chloro pyridine instead. Pivotal to the one-pot procedure with 2-chloro pyridine was the addition of an acid quencher (water or an aqueous NaHCO 3 solution) before adding mCPBA, owing to the high acidity of protonated 2-chloropyridine present in the solution (pK a ¼ 0.49 in H 2 O). 12 By use of this procedure, formal a,b-oxygenation of simple amide 6a was achieved in 93% isolated yield in one pot. The reaction proceeds with a very similar yield on a gram scale. As shown in Scheme 5a, this transformation has some generality and tolerates a range of functional groups, such as esters (10d, 10f), nitriles (10e) and silyl ethers (10c). Electronwithdrawing (such as pCF 3 , 10n) and -donating groups (such as OMe, cf. 10j) are well tolerated at diverse positions of the aryl substituent (Scheme 5a). Heating to 40 C was found to be generally benecial during the epoxidation event to enhance conversion in cases were the aromatic ring is electronically impoverished. 11 The use of aqueous NaHCO 3 instead of water enables successful reaction for acid-sensitive substrates (e.g. TBS protected alcohol, cf. 10c) or sensitive products (electron rich aromatics in proximity to the formed epoxide, cf. 10j). Diastereoselectivity was found to be excellent in all cases: the only case in which the other diastereoisomer was detected in the crude reaction mixture (in traces by 1 H NMR, d.r. > 25 : 1) is the sterically congested product 10b. These epoxides can serve as precursors to more elaborated architectures as depicted in Scheme 5b. NaHMDS-mediated deprotonation of 10e, which was obtained in good yield using the present protocol, led to diastereoselective intramolecular epoxide opening. 13 The resulting tertiary alcohol 22, which contains three contiguous stereogenic centers, was obtained as a single diastereoisomer.

One pot b-oxidation and oxidative C-C bond cleavage
Interestingly (Scheme 6a), when the acid scavenger was omitted in the domino a,b-oxidation of amide 6a, a-ketoamide 23a was obtained as the major product, accompanied by trace amounts of a-acetoxyamide 24a and b-ketoamide 9a. Selectivity can be steered exclusively towards b-ketoamide 9a by adding rst a non-aqueous proton-scavenger (Na 2 HPO 4 ) and then acid (tri-uoroacetic acid, TFA). 15 When the puried b-ketoamide 9a (isolated in 88% yield) was treated with mCPBA in DCM, we observed a-ketoamide 23a as the main product with the a-acetoxy derivative 24a as a side product. We thus propose a mechanistic scenario (Scheme 6b) whereby acid-induced Meinwald rearrangement 15 of epoxide 10a delivers ketone 9a. Baeyer-Villiger oxidation of the latter with mCPBA (preferential migration of the benzylic substituent) 16 combined with nucleophilic attack of mCPBA anion/Kornblum-DeLaMare rearrangement 17 accounts for the formation of products 23a and 24a. In particular, the b-oxidation of an amide to a b-ketoamide (6a / 9a) is, to the best of our knowledge, an unknown transformation with potential synthetic utility.

Synthesis of 2-furanones
When combined with allylic oxidation, 18 the mild dehydrogenation chemistry described herein results in a one-step synthesis of butenolides from simple carboxamides (Scheme 7). The oxidation is most likely followed by nucleophilic displacement of the R-O-Se-OH functionality via the amide carbonyl. 19 The stereoretention of the double bond geometry during oxidation is noteworthy, and the absence of products with inversion of the double bond conguration can be rationalised by the transition state of the [2,3] rearrangement of intermediate 26 (ref. 20). The reaction enables, for instance, the single-step preparation of the antifungal natural product incrustoporine 11q (or analogues such as 11g). 21 One pot b-thiolation Finally, when combined with the addition of thioacetic acid under basic conditions, the chemistry reported herein results in formal direct b-thiolation. 22 As shown in Scheme 8, several derivatives of relevant drugs and biologically active compounds could be thus converted into their b-thiolated analogues. Namely, the dimethylamides of Ibruprofen, Naproxen, Flubriprofen and Ketoprofen all underwent b-thiolation in good to quantitative yields (cf. Scheme 6,8r,s,t,v). Moreover, this chemistry allows interconversion of proteogenic aminoacid derivatives: an amide derivative of alanine 6u was converted into the corresponding cysteine derivative 8u in 76% yield. The metal-free character of these transformations and the substrate complementarity to metal-catalysed processes are worthy of note.

Conclusion
In conclusion, we have shown herein that a facile and chemoselective dehydrogenation event can be leveraged to achieve a plethora of one-pot oxidations/functionalisations of carboxamides. This results in formal direct a,b-epoxidation, direct conversion to aor b-ketoamides at will and the one-step interconversion of a simple linear amide into butenolides by formal a,b,g-oxidation. The ability to deploy this chemistry in a formal b-thiolation hints at its potential utility for late-stage derivatization.

Conflicts of interest
There are no conicts to declare.

Acknowledgements
Funding of this work by the Austrian Science Fund (FWF, P30226) and the European Research Council (ERC, CoG 682002 VINCAT) is acknowledged. We thank the University of Vienna Scheme 6 (a) Alternative pathway towards different ketoamides. See ESI † for detailed reaction conditions. (b) Proposed mechanism.
for its continued and generous support of our research programs. Dr J. Li (Univ. Vienna) is acknowledged for the discovery of the dehydrogenation reaction. Dr W. Zawodny and Dr M. Vayer (both Univ. Vienna) are gratefully acknowledged for proofreading and editing.