GPI0363 inhibits the interaction of RNA polymerase with DNA in Staphylococcus aureus

We previously reported a therapeutically effective spiro-heterocyclic compound, GPI0363, that inhibits the transcription of Staphylococcus aureus via the primary sigma factor of RNA polymerase, SigA. Here, we demonstrated that GPI0363 shares no cross-resistance with the clinically used RNA polymerase inhibitors rifampicin and fidaxomicin. Furthermore, we found that GPI0363 bound to SigA of both GPI0363-susceptible and resistant strains, and inhibited the interaction of the RNA polymerase holoenzyme with DNA. In addition, the gene expression patterns following GPI0363 treatment were different from those following rifampicin treatment. These findings suggest that GPI0363 has a unique mechanism of action and can serve as a promising lead molecule to develop staphylococcal RNA polymerase inhibitors.


Introduction
Bacterial RNA polymerase (RNAP), an enzyme involved in gene transcription and expression, 1 is an attractive target for antimicrobials, 2 mainly because it is conserved among prokaryotes. The eukaryotic transcription system, which comprises of RNA polymerases I, II, and III, typically differs from the prokaryotic transcription system; thus, selective toxicity to prokaryotes can be achieved by inhibiting RNAP. Two antimicrobial agents, rifampicin and daxomicin, are currently in clinical use; rifampicin acts by inhibiting the extension of short RNA products, 3 and daxomicin inhibits the interaction of RNAP with promoter regions of DNA. 4 Although RNAP inhibitors are thought to be broad-spectrum antimicrobial agents, 5,6 narrowspectrum RNAP inhibitors are also reported. 7,8 Nonetheless, the identication of new RNAP inhibitors with novel mechanisms is critical for antimicrobial drug development to overcome the growing problem of drug-resistance.
We previously reported a therapeutically effective spiroheterocyclic antimicrobial, GPI0363 (Fig. 1a), identied by screening in a silkworm infection model. 8,9 It inhibited transcription in Staphylococcus aureus by binding to the primary sigma factor, SigA, and a single point mutation (D201N) in SigA was responsible for resistance to GPI0363. 8 However, the mechanism of how it inhibits transcription was not fully understood. Here, we elucidated that GPI0363 inhibited the interaction of RNAP holoenzyme with DNA and has a mechanism distinct from that of clinically used antibiotics.

GPI0363 does not have cross-resistance with clinically used RNAP inhibitors
Given that GPI0363 targets RNA synthesis, we evaluated whether GPI0363 has cross-resistance with clinically used RNAP inhibitors. We generated spontaneous S. aureus strains resistant to rifampicin (R1-R5) and daxomicin (F1-F5) and determined their susceptibilities to GPI0363. We also determined the drug susceptibilities of the GPI0363-resistant strain G2-1, which was generated by phage transduction and has a D201N mutation in SigA. 8 The strains resistant to rifampicin and daxomicin were susceptible to GPI0363 and vice versa (Table 1). We then analyzed the whole genome sequence of the rifampicinand daxomicin-resistant strains and found that all the rifampicin-resistant strains had a single amino acid substitution, H418Y, in the RNAP b subunit RpoB (Table 1). The RpoB H418Y mutation is reported in several rifampicin-resistant strains 10,11 and is known to confer resistance by decreasing the binding affinity of rifampicin to the RNAP. 10 Similarly, among ve daxomicin-resistant strains, four (F1, F2, F4, and F5) had the same single amino acid substitution Q1061K in the RNAP b subunit, RpoB. This mutation is also reported in various daxomicin-resistant strains. 12,13 The remaining one strain (F3) harbored an R80S substitution in the RNAP b 0 subunit, RpoC (Table 1). RpoC R80 is one of six amino acid residues involved in hydrogen-bond formation with daxomicin and a mutation at this position is responsible for resistance to daxomicin. 4 These GPI0363 binds to mutant SigA from GPI0363-resistant strain The absence of cross-resistance of GPI0363 with clinically used RNAP inhibitors led us to study the mechanism of GPI0363 in detail. In our previous study, we found that GPI0363 inhibits transcription in S. aureus by binding to the primary sigma factor SigA of the RNA polymerase, and a D201N mutation in SigA is responsible for resistance to GPI0363. 8 We hypothesized that the mutant SigA might have little or no binding affinity with GPI0363, thus conferring resistance. Therefore, as a rst step to study the mechanism of action, we checked the binding of GPI0363 to the wild-type and mutant SigA. GPI0363 bound to both SigAs with a 0.8 ratio of GPI0363 eluted from the beads with SigA D201N to wild-type SigA (ESI Fig. 1 †). The binding of GPI0363 to SigA from both the susceptible and resistant strains implied that resistance to GPI0363 could not be explained by the loss of binding capacity and D201 in SigA might not be involved in the binding to GPI0363. On this basis, we speculated that GPI0363 acts at latter stepsbinding of SigA to the RNAP core enzyme or interaction of the RNAP holoenzyme with DNA.

GPI0363 inhibits the interaction of RNA polymerase with DNA aer binding to SigA
To examine the effect of GPI0363 in the different transcription steps, we rst established an in vitro transcription assay using three different promoters of the dra, pB (ESI Fig. 2 †), and aA 8 genes. We found that GPI0363 inhibited transcription from these promoters in a similar and dose-dependent manner. We selected the aA promoter for our subsequent experiments as it showed a clear promoter-specic band with less background among the three promoters. To test whether GPI0363 inhibits the binding of SigA to the RNAP core enzyme, we treated SigA with GPI0363, followed by the addition of the Escherichia coli RNAP core enzyme and performed in vitro transcription. We found that the transcription inhibition from this hybrid RNAP (Fig. 1b, and ESI Fig. 3 †) was similar to that from the S. aureus .078 mg mL À1 ) before incubation with E. coli RNAP core enzyme followed by in vitro transcription. The transcripts were extracted, electrophoresed, and visualized by autoradiography. (c) Effect of GPI0363 on promoter-specific transcription before and after S. aureus RNAP (Sau RNAP) was incubated with DNA. Sau RNAPs from the wild-type and GPI0363-resistant mutant were treated with GPI0363 (0.3 mg mL À1 ) before or after incubation with template DNA followed by in vitro transcription. The transcripts were extracted, electrophoresed, and visualized by autoradiography. Data represent mean AE SD of three independent experiments and were analyzed by Student's ttest using Prism for Mac OS X, version 5.0d (GraphPad Software). Significant differences are indicated by asterisks (***p ¼ 0.002). Band intensities were measured by ImageJ v1.47 by taking the band intensity without GPI0363 as 100%. Gel images are shown in ESI Fig. 3, 5 and 6. † To test the ability of GPI0363 to inhibit the interaction of the RNAP holoenzyme with DNA, we added GPI0363 before and aer incubation of the Sau RNAP holoenzyme with DNA and assessed the transcription in vitro. Stronger inhibition was observed when GPI0363 was added to the wild-type Sau RNAP before incubation with the DNA, whereas the transcription inhibition from mutant Sau RNAP was not affected by the different orders of addition (Fig. 1c, ESI Fig. 5 and 6 †).
GPI0363 did not intercalate into the double-stranded DNA Next, to test whether GPI0363 interacts with DNA itself, we added salmon sperm DNA to the assay medium and evaluated the effect of external DNA on the minimum inhibitory concentration (MIC) of GPI0363. The MIC values of GPI0363 did not change in the presence or absence of external DNA (Table 2), while those of actinomycin D, a known DNA intercalator, 14 increased in the presence of the external DNA, suggesting that GPI0363 does not intercalate with DNA.
Taken together, our ndings suggest that GPI0363 inhibits the interaction between the RNAP holoenzyme and DNA by binding to SigA. To further gain an insight into the function of the mutation responsible for GPI0363 resistance, we aligned the S. aureus SigA with the SigAs from Gram-positive Bacillus subtilis, Gram-negative E. coli, and Gram-negative Deinococcus-Thermus Thermus thermophilus. The analysis revealed that D201 was in the universally conserved region 2.4 of SigA (Fig. 2). This region harbors allele-specic suppressors of promoter mutations in the À10 promoter region, 15 thus involved in promoter recognition [16][17][18] suggesting that D201N mutation affects the promoter-specic binding capacity of RNAP. A detailed structural analysis of the effect of this mutation and determination of the exact binding site of GPI0363 with SigA by co-crystallization is planned for a future study.
GPI0363 treatment alters the S. aureus transcriptome differently than rifampicin To further reveal the difference in the mechanisms of action between rifampicin and GPI0363, we performed RNA-seq analysis aer treating S. aureus Newman with the MICs of the respective drugs. We compared the gene expression patterns on the basis of signicant fold-expression changes [minimum fold difference: 2, false discovery rate (FDR) p value: <0.05] of GPI0363treated and rifampicin-treated samples in comparison with the non-treated samples. Comparative analysis of downregulated (Fig. 3a) and upregulated (Fig. 3b) genes indicated that a large number of differentially expressed genes was distinct among the two drugs. We further performed a functional categorization of the upregulated and downregulated genes according to the KEGG pathway and found that the two drugs differentially affected the metabolic processes of S. aureus (Fig. 3c).
While genetic information processing was only affected by GPI0363, cellular processes, metabolism of terpenoids and polyketides, and metabolism of other amino acids were only affected by rifampicin. Nucleotide metabolism was mostly upregulated by rifampicin and mostly downregulated by GPI0363, and energy metabolism was more affected by GPI0363 than by rifampicin. Although changes in the gene expression patterns may have arisen as secondary effect, the results obtained here imply that the target genes of GPI0363 may be different from those of rifampicin.

Chemicals and reagents
All the chemicals and reagents used in this study were of analytical grade. GPI0363 was obtained from the chemical library of the Drug Discovery Initiative at the University of Tokyo and its purity was conrmed by ultra-performance liquid chromatography-high

Microorganisms and culture conditions
The bacterial strains used in this study are summarized in ESI Table 1. † S. aureus strains were grown in TSB and E. coli was grown in Luria Bertani medium. MHB supplemented with cations was used to determine antimicrobial susceptibility.

Generation of spontaneous strains resistant to antibiotics
S. aureus RN4220 was cultured overnight on TSB at 37 C with ambient air at 200 rpm. An aliquot (100 mL) of the overnight culture was then spread on TSB agar plates containing 4 and 8 mg mL À1 rifampicin, or 8 and 16 mg mL À1 daxomicin. The plates were incubated at 37 C overnight, and single colonies were isolated.

Binding of SigA with GPI0363
SigA from the wild-type and GPI0363-resistant S. aureus RN4220 strain was expressed in E. coli to obtain wild-type SigA and SigA D201N , respectively as previously described. 8 Binding of SigA with GPI0363 was evaluated according to the previously reported method. 8 Briey, TALON® magnetic beads pre-charged with cobalt were equilibrated with 50 mM Tris buffer, pH 7.5 followed by incubation with wild-type SigA or SigA D201N in the presence or absence of GPI0363. GPI0363 was pretreated with bovine serum albumin (0.5 mg mL À1 ) before incubating with SigA and beads. The resulting beads were washed with the same buffer, separated, and eluted with 50% acetonitrile + 0.1% tri-uoroacetic acid, and analyzed by high-performance liquid chromatography (HPLC) with a TSKgel a-M size exclusion column (7.8 mm ID Â 30 cm, 13 mm; TOSOH) and a mobile phase comprising 50% acetonitrile + 0.1% triuoroacetic acid at a ow rate of 0.5 mL min À1 .

In vitro transcription assay
DNA templates were prepared with primers as indicated in ESI Table 2 † using PrimeSTAR Max DNA Polymerase (TaKaRa, Japan). Purication of S. aureus RN4220 RNAP and in vitro transcription were performed as previously described. 8,19 Briey, 0.2 mL of the RNAP fraction was added to the reaction buffer (nal volume 25 mL) containing 40 mM Tris-acetate (pH 7.9), 100 mM NaCl, 5 mM MgCl 2 , 0.2 mM DTT, 100 mg mL À1 bovine serum albumin, and 0.5 units of RNase inhibitor. GPI0363 was then added and the mixture was incubated for 5 min at 37 C, before initiating the transcription by the addition of NTP mixture [0.25 mM each of ATP, CTP, GTP; 0.015 mM UTP, and 10 mCi of [a-32 P] UTP with template DNA (a 406-bp fragment of the aA gene, a 528-bp fragment of the pB gene, or a 486-bp fragment of the dra gene including the promoter regions)]. The reaction was allowed to proceed for 5 min at 37 C and then stopped by the addition of 100 mL ice-cold stop solution (0.4 M ammonium acetate, 20 mM EDTA, 0.3% sodium dodecyl sulfate, 4 mg tRNA). Transcripts were extracted in phenol : chloroform : isoamyl alcohol (125 : 24 : 1, v/v/v); electrophoresed on 7 M urea, 6% polyacrylamide gels, and visualized by autoradiography using Typhoon FLA 9000 (GE Healthcare, Japan). The band intensities were quantied using ImageJ soware (v1.47, NIH, USA). 20 To examine the effect of GPI0363 in the interaction of RNAP and DNA, a reaction mixture containing the RNAP fraction was incubated at room temperature for 5 min with template DNA before the addition of GPI0363.
For experiments with E. coli core RNAP, S. aureus SigA was treated with GPI0363 for 10 min at room temperature, followed by incubation with reaction mixture containing the E. coli RNAP core enzyme at room temperature for 10 min. The reaction was started with the addition of the mixture of NTPs and aA template DNA. For in vitro transcription from the E. coli RNAP holoenzyme, a 450-bp long DNA fragment from pBR322 was used as the template.
In vitro susceptibility test of S. aureus to antimicrobials S. aureus MSSA1 and RN4220 were grown on Luria Bertani (tryptone 10 g L À1 , yeast extract 5 g L À1 , NaCl 10 g L À1 , pH 7.0) agar plates at 37 C with ambient air at 200 rpm overnight. The MIC was determined by broth microdilution assay in cation-adjusted MHB according to previously described methods. 21,22 For determination of the MIC in the presence of external DNA, we added salmon sperm DNA (deoxyribonucleic acid sodium salt from salmon testes, Sigma Aldrich) into the MHB medium, and actinomycin D (Sigma Aldrich) was used as a positive control.

RNA extraction from S. aureus
The overnight culture of S. aureus Newman was diluted 100 fold and incubated at 37 C with aeration until the OD 600 was 1.0. The culture was treated with GPI0363 (MIC-4 mg mL À1 ) or rifampicin (MIC-0.004 mg mL À1 ) for 30 min. To stabilize the RNA, 4 mL of RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) was added to 2 mL bacterial culture. Total RNA was extracted using the RNeasy® Protect Bacteria Mini Kit (Qiagen) according to manufacturer's protocol using QIAcube (Qiagen) with lysis in 10 mM Tris HCl, 1 mM EDTA buffer (pH 8.0) containing 1 mg mL À1 lysostaphin at 37 C for 30 min.

Library preparation for RNA-sequencing and analysis of data
Ribosomal RNA in the total RNA was depleted using MICROBExpress™ Bacterial mRNA Enrichment Kit (Thermo Fisher Scientic, Carlsbad, CA, USA) according to the manufacturer's protocol and rRNA depletion was conrmed using an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Whole transcriptomic libraries for RNA sequencing were prepared according to the instructions for the Ion Total RNA-seq Kit v2 (Thermo Fisher Scientic). Briey, rRNA-depleted RNA was fragmented using RNase III, followed by reverse transcription and amplication. The size distribution and yield of the amplied library was conrmed using the Bioanalyzer 2100. The libraries were then enriched in an Ion PI Chip v2 using the Ion Chef (Thermo Fisher Scientic), and the subsequent sequencing was performed in the Ion Proton System (Thermo Fisher Scientic). All data were analyzed using CLC Genomics Workbench ver. 12.0 (Qiagen Bioinformatics, Aarhus, Denmark). Reads were aligned to the Newman genome allowing a minimum length fraction of 0.9 and minimum similarity fraction of 0.9. Genes with a signicant false discovery rate (p < 0.05) and minimum two-fold-expression were classied as having signicantly different expression.

Genomic DNA extraction and whole-genome sequence analysis
Genomic DNA of the strains was isolated from overnight cultures using a DNeasy Blood and Tissue kit (Qiagen) by QIAcube (Qiagen) according to the manufacturer's recommendations. The library was prepared using 100 ng of the genomic DNA quantied using a Qubit 3.0 uorometer (Thermo Fisher Scientic) as described previously. 23,24 Briey, genomic DNA was fragmented using an Ion Shear™ Plus Reagent Kit (Thermo Fisher Scientic) and a 400 base-read barcoded library was prepared using the Ion Xpress™ Plus Fragment Library Kit (Thermo Fisher). E-Gel SizeSelect™ (Thermo Fisher Scientic) was used for the 400 base size selection, and the library was amplied by polymerase chain reaction. The quality, quantity, and size distribution of the libraries were determined using an Agilent Bioanalyzer 2100 (Agilent Technologies), and enriched using an Ion 318™ Chip v2 in Ion Chef (Thermo Fisher). Sequencing was performed in the Ion Personal Genome Machine (Thermo Fisher Scientic). The reads were then mapped to the S. aureus NCTC8325 genome, the parent strain of S. aureus RN4220, using CLC Genomics Workbench ver. 12.0 (Qiagen Bioinformatics) followed by the extraction of variants specic to the drug-resistant strains.

Conclusions
Identifying antimicrobial agents effective against drug-resistant pathogens and understanding how these molecules exert their actions is critical towards combating the growing spread of multi drug-resistant pathogens. Recently, while screening of antimicrobial agents against methicillin-resistant S. aureus, we found that GPI0363 inhibits promoter-specic transcription by binding to the primary sigma factor of RNAP, SigA. 8 The bacterial RNAP holoenzyme binds to the promoter region of template DNA and forms an open complex by separating the double helix of the template, followed by transcription initiation. 25 In this manuscript, we investigated the mechanism of action of GPI0363 in detail and demonstrated that the antistaphylococcal activity of GPI0363 is the result of transcription arrest caused by the inhibition of the interaction of the RNAP holoenzyme with the promoter region. Several antimicrobial agents, such as myxopyronin, corallopyronin, ripostatin, and daxomicin, target the switch region of RNA polymerase and interfere with the RNAP-DNA interaction. 4,26 GPI0363 has a unique mode of action by binding to SigA. Moreover, the lack of cross-resistance of GPI0363 with rifampicin and daxomicin implies that its mechanism is distinct, which was further evidenced by the different transcriptomic proles upon treatment with GPI0363 and rifampicin. Future studies should focus on elucidating the exact binding site and the sequence of events taking place in the S. aureus transcription machinery aer GPI0363 treatment. In addition to being a promising lead molecule for the development of narrow-spectrum antimicrobial agents, GPI0363 can serve as a chemical biology tool for fundamental studies of SigA-dependent transcription.

Conflicts of interest
There are no conicts to declare.