Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

Christof Domnick; Gregor Hagelueken; Frank Eggert; Olav Schiemann; Stephanie Kath-Schorr

doi:10.1039/C8OB02597E

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Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition†

Christof Domnick,^a Gregor Hagelueken,^b Frank Eggert,^a Olav Schiemann

^b and Stephanie Kath-Schorr

*^a

Author affiliations

* Corresponding authors

^a Life & Medical Sciences Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany
E-mail: skath@uni-bonn.de

^b Institute for Physical and Theoretical Chemistry, University of Bonn, Wegelerstr. 12, 53115 Bonn, Germany
E-mail: schiemann@pc.uni-bonn.de

Abstract

The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.

This article is part of the themed collections: Chemical Biology in OBC and New Talent

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Article information

DOI: https://doi.org/10.1039/C8OB02597E
Article type: Communication
Submitted: 19 Oct 2018
Accepted: 27 Nov 2018
First published: 28 Nov 2018

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Org. Biomol. Chem., 2019,17, 1805-1808

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Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

C. Domnick, G. Hagelueken, F. Eggert, O. Schiemann and S. Kath-Schorr, Org. Biomol. Chem., 2019, 17, 1805 DOI: 10.1039/C8OB02597E

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Organic & Biomolecular Chemistry

Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition†

Abstract

Supplementary files

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Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

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