Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging † Journal of Materials Chemistry B COMMUNICATION

The short shelf-life of water-soluble quantum dots (QDs) due to colloidal instability represents a major drawback to their exploitation. This work examines the colloidal stability of PbS nanoparticles capped with dihydrolipoic acid–polyethylene glycol (DHLA–PEG) ligands terminated with functional groups such as –NH 2 , –COOH, OMe and –N 3 . and their application for in vivo imaging. We prove a mechanism of colloidal instability and develop a strategy to produce for the first time stable PEG-capped PbS quantum dots with high quantum yield and optical emission in the first and the second near-infrared (NIR) windows of low absorption of biological tissues. The NIR imaging of in vivo biodistribution is demonstrated at wavelengths 4 1000 nm, with ben-efits of reduced tissue absorption and light scattering. The stability, biocompatibility and potential for further QD functionalization open up realistic prospects for non-invasive bioimaging applications. deep and in vivo NIR imaging of QD biodistribution in mice.

2][3] Various nanomaterial systems have been explored for medical imaging, including metal nanoparticles, 4,5 upconverting nanoparticles 6 and semiconductor quantum dots. 7Of particular interest is the development of biocompatible colloidal quantum dots (QDs) with optical emission tuneable in the near-infrared (NIR) wavelength range of reduced light scattering and low absorption of biological tissues, [8][9][10] which can be achieved with IV-VI-based semiconductor materials (e.g.PbS, PbSe). 9,11The important role of surface ligands for controlling and designing functional nanomaterials has been widely acknowledged. 12,13It has also been shown that the efficiency of ligand binding to the surface of a nanocrystal has a strong influence on QD polydispersity, quantum yield and photostability. 14,15Polymer-based capping ligands that are biologically inert, e.g.7][18][19] However, the development of capping ligands that can effectively passivate the surface of a nanocrystal, provide longevity and colloidal stability, do not alter its optical properties and allow controllable attachment of functional molecules remains a challenge. 13,202][23][24] Despite promising optical and electronic properties, the technological exploitation of these materials has stalled due to their short shelf-life caused by colloidal instability.
Here we report the development of nanoparticles that combine the beneficial biocompatibility and stability offered by DHLA-PEG-ligands with the tuneability of the emission of PbS nanocrystals in the NIR wavelength range of low absorption of biological tissues.We report on the stability of DHLA-PEG ligands and their utilization for surface passivation of quantum dots.We investigate the reasons for previously reported colloidal instability and short shelf-life of colloidal solutions of thiollinked, PEG-capped nanoparticles.We report time-dependent nuclear magnetic resonance (NMR) studies of ligand molecules and QDs, and suggest a strategy for the synthesis of stable nanoparticle solutions.The DHLA-PEG ligands, modified with functional groups including ÀOMe, ÀNH 2 , ÀCOOH and ÀN 3 , are used to synthesize colloidal PbS QDs in aqueous solution with high quantum yield (B30%) and with room temperature photoluminescence (PL) tuneable by the QD core size in the NIR wavelength range, hence enabling enhanced light penetration through deep tissues and in vivo NIR imaging of QD biodistribution in mice.These results open up prospects for use of PbS-DHLA-PEG QDs in biomedical applications where functional groups can be used for the controllable attachment of targeting molecules, such as peptides, small proteins or antibodies.
The ligands used in this work are composed of three parts: DHLA, a PEG400 linker, and a functional group, R*, such as ÀOMe, ÀNH 2 , ÀCOOH and ÀN 3 , 22,25 corresponding to ligand molecules LOMe, LNH 2 , LCOOH and LN 3 , respectively.PEG400 is a hydrophilic molecule that provides water solubility, pH stability and biocompatibility of the QDs, and can be easily changed for a different chain length from PEG200 to PEG2000.The stability and aging of ligand molecules has been assessed by NMR timecourse (Fig. 1 and ESI, † SI2).Our 1 H-NMR studies indicate that for the LNH 2 and LN 3 molecules, the spontaneous oxidation of the DHLA and reformation of the disulfide bond of its parent compound lipoic acid (LA) takes place with B70% conversion over 48 h (Fig. 1a).A clear resurgence of the proton signals at B2.45 ppm (H b* ) and at B3.18 ppm (H a*/a 0 * ) (see Fig. 1b) is observed in LNH 2 , which are typical of the 5-membered dithiolane ring containing the disulfide bond.At the same time, the intensities of peaks at 2.71 ppm (H a/a 0 ) and 2.94 ppm (H c ), characteristic of DHLA, decrease.The change of intensities is described by the first order kinetic regime with comparable time constants (inset in Fig. 1b), as expected for the interconversion reaction where DHLA is oxidized to LA.These results indicate the inherent instability of LNH 2 and LN 3 , which is likely due to the interaction between the amine moiety and the thiols. 26,27n the other hand, LOMe and LCOOH are stable and do not undergo spontaneous oxidation (Fig. 1c and ESI, † SI2) over the same observation period.The formation of the disulfide bond prevents the ligand binding to the nanoparticle surface, thus affecting the colloidal stability of QDs.
Particular attention to the purification of the ligand mixture is paramount for the stability of aqueous QD solutions.During the ligand synthesis, the Staudinger reduction uses triphenyl phosphine (Ph 3 P), generating triphenylphosphine oxide (Ph 3 P(O)) as a by-product.The 31 P NMR shows a clear peak at À29 ppm, confirming the presence of Ph 3 P(O) (ESI, † SI2).The Ph 3 P(O)/Ph 3 P present in the reaction mixture can compete with the DHLA-PEG as a QD capping molecule, thus altering the aqueous solubility of the nanocrystals. 28e have used these DHLA-PEG capping ligands to produce PbS quantum dots either in aqueous solution or in phosphate buffered saline (PBS) (Fig. 2a) with sizes tuneable by the molar ratio of Pb : S. 29 For the Pb : S ratio of 1 : 0.3, the QDs have average diameter d PbS = 3.2 AE 0.8 nm (Fig. 2b).The dark field TEM studies reveal the presence of a thick carbon-based amorphous layer with non-uniform depth surrounding the PbS nanocrystal.The inter-particle distance value of Z2 nm indicates interlocking of the DHLA-PEG between neighboring nanocrystals on the surface. 30,31The average hydrodynamic diameter of the QDs measured using dynamic light scattering (DLS) is d QD = 11 AE 1 nm, which is close to our estimate d QD = d PbS + 2l E 13 nm, where l E 5 nm is the length of the extended ligand molecule.The QD solutions prepared with molar ratio of Pb : S = 1 : 0.3, have room temperature photoluminescence centered at l PL = 920 nm, which is independent of the type of ligand and/or ligand mixture used for surface passivation (Fig. 2c).
By varying the Pb : S ratio from 1 : 0.3 to 1 : 0.6 we produce quantum dots with a diameter ranging from d PbS E 3.2 nm to 5.5 nm, with corresponding PL emission tuneable in the NIR range from 920 nm to 1220 nm, respectively (Fig. 3a).The PL peak position and intensity are comparable for all ligands used, irrespective of the specific terminating group (ÀOMe, ÀNH 2 , ÀCOOH and ÀN 3 ) and only small time-dependent shift is observed due to Ostwald ripening (Fig. 2c).All PbS QD solutions have quantum yields of about 30% as measured using the integrated sphere method (Edinburg Instrument spectrometer FLS980 equipped with 120 mm diameter integrated sphere), which is higher than that previously reported for other types of PbS QDs. 32We note that stable colloidal solutions of PbS QDs can also be produced with mixed ligands, e.g.LNH 2 : LCOOH in a 3 : 1 molar ratio (inset of Fig. 2c).The mixing of the ligands has a potential benefit for the controlled attachment of different molecules.This in turn could allow greater selectivity, e.g.attachment of complementary targeting molecules.Also, the control of the ratio of functional groups on the QD surface allows tuneability of the surface charge from positive for PbS-LNH 2 (z-potential B 2.6 AE 3.2 mV) to negative for PbS-LCOOH QD (z-potential B À41.3 AE 5.5 mV).
The QD solutions prepared using freshly synthesized ligands have long-term colloidal stability (stored at T = 4 1C under nitrogen atmosphere) over a period of at least 9 months. 1H-NMR studies of these QD solutions show a clear resonances at 2.71 ppm (H a/a 0 ) and 2.94 ppm (H c ), characteristic for DHLA.In stark contrast, when ligands were stored prior to the nanocrystal synthesis, QD solutions precipitated within o10 days and the 1 H-NMR spectra of the supernatant confirmed the formation of the dithiolane ring on the ligand molecules.Hence the instability is due to lack of surface passivation by the unreactive oxidized ligand molecules.
The potential of these QDs for bioimaging applications was assessed by studies of the QD NIR emission penetration through biological tissues.For these studies we selected the QD solutions with PL at l > 1000 nm to benefit from low absorption of biological tissues and lower light scattering, thus achieving deeper tissue penetration of the emitted light.To account for the composition of different tissues, we prepared mouse brain and liver tissue slices with thicknesses ranging from t = 0.2 mm to 4 mm.The slices were placed over two glass capillaries of diameter d = 1 mm containing PbS-LNH 2 solutions with PL emission of comparable intensity centered at l PL B 1050 nm (QD1) and at l PL B 1220 nm (QD2) (Fig. 3a).Fig. 3b and c illustrate representative optical images of 0.5 mm thick brain and liver slices, respectively, placed over the capillaries.The corresponding PL maps were acquired at l = 1000 AE 25 nm (middle images) and at 1300 AE 25 nm (bottom images).They demonstrate that the NIR fluorescence of the QDs can be detected through all studied tissues.However, the observed decrease of light intensity through tissues is significantly greater for high-blood-content tissues, such as liver, compared to brain.For example, for brain tissues of thickness t = 0.5 mm, no measurable decrease of optical signal was detected compared to capillary only.For the same thickness of liver tissue, about 5-times decreased intensity was observed at l = 1000 AE 25 nm and 2-times decreased intensity at l = 1300 AE 25 nm (Fig. 3d).The use of longer wavelengths enables better imaging through liver, with B1.5 times higher resolution achieved for all tissue thicknesses (Fig. 3c and ESI, † SI3) due to reduced scattering. 8,33We also note that a signal to noise ratio of Z3 was achieved for liver tissue of thicknesses up to 4 mm.The observed signal penetration depth confirms the suitability of these QDs for non-invasive imaging.
In order to guide the in vivo studies, we assessed the cytotoxicity of these QDs in vitro by MTT assay.Prior to cell culture and in vivo studies, we dialyse the QD solutions against PBS and lower the pH.We note that this process does not alter the colloidal stability and photostability of the QDs.Previously, PbS QDs were reported to induce greater toxicity in tumorigenic cell lines compared to normal cells. 29,34We selected three human derived carcinoma cell lines: MDA-MB-231 and MCF-7 breast, and HCT-116 colorectal cells.Assessment after 72 h exposure of cells to PbS QDs demonstrated that in all three cell lines, neither cell viability nor cell growth were compromised at PbS QD concentrations r20 mg mL À1 (see ESI, † SI3).The acute toxicity studies over a period of 30 days have not revealed any QD-induced toxic effects in mice. 34No indication of damage to the organs/tissues was found in histological examination of internal organs at the end of the study, confirming the stability of QDs in vivo and the absence of release of heavy metal ions from the QDs.
The in vivo biodistribution of QDs was observed using NIR fluorescence.Mice were injected via tail vein with 30 mL of 0.2 mM PbS QD solution, corresponding to a final concentration of approximately 10 mg mL À1 (2-times lower than the GI50 value).Fig. 4a illustrates typical NIR images (l > 1000 nm) of PbS QDs (LNH 2 ) in blood vessels in a mouse head immediately after the injection.The fluorescence in the brain vessels fades within a few minutes and the emission in not detectable upon inspection of the brain ex vivo 48 h after injection, indicating that these QDs do not pass the blood-brain barrier.
Within 1 hour, measurable NIR fluorescence was observed in the areas of liver and spleen.Fig. 4b illustrates a typical biodistribution of the PbS QDs, as detected with NIR fluorescence 24 hours after the QD injection.The intensities of the fluorescence signal observed in liver and in spleen are comparable when measured in vivo (see top insets in Fig. 4b) and ex vivo in the corresponding organs (see lower insets of Fig. 4b).In agreement with our tissue penetration studies, the in vivo fluorescence intensity is about 2-times lower compared to the ex vivo value.We note that, as expected, NIR fluorescence was only detected in QD injected animals (see ESI, † SI3).Following 48 h exposure, the intensity of the optical signal from both liver and spleen decreased by a factor of B3 compared to that measured after 24 h exposure.We attribute the observed decrease of fluorescence to gradual clearance of QDs from the body.Indeed in some mice, significant fluorescence was observed in the bladder area, indicating that QDs are cleared from the body via the urinary tract.There is also some indication that after 48 h some QDs might be present in lymph nodes, as was also reported previously. 33,35We exclude the possibility that the decrease in fluorescence intensity is due to interactions of QDs with proteins in blood/serum (e.g. with albumin), as we prepare our QDs with excess ligands to ensure efficient passivation of the surface in order to eliminate such interactions.Also, even in the case of QD binding to proteins in serum, the formed complexes are reported to be both soluble and photostable. 36e note that over the duration of the study, the QDs were well tolerated by mice.No distress or noticeable physiological changes were observed.Also, mice organs (liver, spleen, brain, kidneys, lungs and heart) were inspected after 24 h and after 48 h, and no visible morphological changes were observed.Ex vivo, measurable fluorescence was detected only in spleen and liver (lower insets in Fig. 4b).Importantly, no noticeable fluorescence was detected in kidneys, indicating that QDs do not accumulate and are well cleared via the kidneys.No noticeable changes in QD biodistribution and in fluorescence intensity were observed for QDs capped with ligands terminated with different functional groups.
In our preliminary studies, we have successfully attached folic acid and cyclic RGD peptides, as cellular targeting ligands, using -NH 2 and -N 3 terminated ligands, respectively, and produced nanoparticles with these functionalized ligands.Further work is underway to explore the targeting efficiency of these functionalized nanocrystals in tumour bearing mice and to fully assess the potential of these QDs for bioimaging applications.

Conclusions
In conclusion, we have identified and addressed the causes that trigger the instability of colloidal nanoparticles capped with DHLA-PEG-based ligands, i.e.LOMe, LNH 2 , LN 3 , LCOOH or their mixtures.Also, we have successfully used these molecules to produce stable and biocompatible PbS QDs with optical emission tuneable in the near-infrared wavelength range.The QDs are well tolerated by mice in vivo and their emission can be imaged in the second NIR optical window.Our findings are transferable to other types of semiconductor and metal nanoparticles and offer potential for further functionalization of the nanocrystals for a wide range of biomedical applications.

Fig. 1
Fig. 1 (a) Reaction scheme of the oxidation of the dithiols to the dithiolane ring; R is the remaining part of lipoic acid-PEG400-terminating group.Representative 1 H-NMR spectra of LNH 2 (b) and LCOOH (c) for freshly synthesised ligands and following 48 h storage, with corresponding time dependence of the intensities of selected resonances.

Fig. 3
Fig. 3 (a) Room temperature PL spectra of PbS-LNH 2 nanocrystals QD1 (orange spectrum) and QD2 (red spectrum).The highlighted areas indicate the wavelength range where PL images through the tissues are acquired.Optical images and corresponding PL images of QD loaded capillary tubes overlaid with (b) brain and (c) liver tissue slices of thickness t = 0.5 mm.PL images were acquired with excitation wavelength l ex = 670 nm and bandpass filters at (1000 AE 25) nm and at (1300 AE 25) nm.(d) PL intensity measured through the liver tissues versus the tissue thickness at l = 1000 nm and l = 1300 nm.Dashed lines are guide to the eye.

Fig. 4
Fig. 4 (a) Photograph of a mouse head and a corresponding NIR fluorescence image of blood vessels in the head immediately after tail vein injection of LNH 2 capped QDs (PL centred at 1050 nm).(b) Whole body and corresponding intensity surface, and ex vivo NIR fluorescence images of liver (left inset) and spleen (right inset) of a mouse 24 h after tail vein injection of LNH 2 capped QDs (PL centred at 1220 nm).NIR images were acquired with excitation laser at l ex = 670 nm (P = 10 W m À2 ), InGaAs CMOS camera and a long pass filter at 1000 nm.