Direct synthesis of aryl-annulated [c]carbazoles by gold(i)-catalysed cascade reaction of azide-diynes and arenes

The gold-catalysed annulation of conjugated alkynes bearing an azido group with arenes gave annulated [c]carbazoles.


Introduction
Carbazoles are important structural motifs that are found in a variety of organic molecules of current interest (Fig. 1). 1 Benzo [c]carbazoles are commonly used in organic light-emitting diodes (OLEDs) owing to their charge-transport properties and thermal stability. carbazoles are the core structures of various bioactive natural products, such as eustifoline-D (furo [2,3-c]carbazole), arcyriaavin A and dictyodendrins (pyrrolo[c]carbazole), and asteropusazole A (indolo [3,2-c]carbazole). 1a Thus, the development of efficient synthetic methods for preparing benzo [c]carbazoles and their heteroaromatic congeners from readily accessible starting materials is an active pursuit in organic chemistry.
The general synthetic approaches to carbazoles including aryl-annulated [c]carbazoles are shown in Scheme 1A and B. 1a,3 Pyrrole ring formation based on a combination of carbonnitrogen and carbon-carbon bond formation provides an efficient route to carbazole synthesis (Scheme 1A). 4,5 Reliable coupling reactions such as the Suzuki-Miyaura, Buchwald, and oxidative coupling reactions can be employed for this purpose. Benzene ring formation using vinyl-or aryl-substituted indoles including Diels-Alder-type reactions, 6 hydroarylation, 7 and related reactions 8 leads to various carbazoles including arylannulated carbazoles (Scheme 1B). However, the double cyclisation approach for synthesising aryl-annulated [c]carbazoles has not been investigated until recently. [9][10][11] We expected that the gold carbenoid-based cascade cyclisation of conjugated diynes would directly provide aryl-annulated [c]carbazoles in a single operation via the sequential cleavage of two aromatic C-H bonds (Scheme 1C). Homogenous gold catalysis has emerged as a powerful tool for atom-economical transformations. 12 The p-acidity of gold catalysts enables the activation of C-C multiple bonds to promote various transformations. Recent investigations using diynes in gold-catalysed reactions have revealed that both conjugated and unconjugated diynes are useful precursors of complex molecules. 13 For example, we recently reported a goldcatalysed formal [4 + 2] reaction between 1,3-diynes and pyrroles for synthesising 4,7-disubstituted indoles (Scheme 2A, n ¼ 0). 14a This reaction proceeded through a double hydroarylation cascade involving the initial intermolecular hydroarylation of 1,3-diyne at the 2-position of pyrrole, followed by intramolecular hydroarylation. When using skipped diynes as substrates, the formal [5 + 2] reaction efficiently proceeded to produce 1,6-dihydrocyclohepta [b]pyrrole derivatives, 14b which can be considered as homologs of 4,7-disubstituted indoles (Scheme 2A, n ¼ 1).
We then turned our attention to synthesising aryl-annulated [c]carbazoles based on gold-carbenoid formation 15 using conjugated diynes. In 2011, Gagosz 16a and Zhang 16b independently reported the gold(I)-catalysed synthesis of indoles bearing an electron-donating group at the 3-position (Scheme 2B). 17 The reaction can be rationalised by the formation of a gold carbenoid intermediate followed by a nucleophilic reaction at the carbenoid moiety. As the coupling partners, alcohols and arenes can be used for the reaction to produce 3-substituted indoles. We envisaged that incorporating gold carbenoid chemistry into diyne cyclisation would provide direct access to aryl-annulated [c]carbazoles (Scheme 3). Thus, the gold(I)mediated nucleophilic attack of the azido group of diyne 1 on the proximal alkyne followed by the elimination of nitrogen would produce gold carbenoid species A. The electrophilic aromatic substitution of benzene-type arenes with A would produce intermediate 2. Finally, intramolecular hydroarylation toward alkynes 18 would occur to produce benzo[c]carbazole 3. The challenge of this strategy is controlling the regioselectivity when using pyrrole-type heteroarenes as the coupling partner: whereas the rst nucleophilic attack at the pyrrole 3-position would produce pyrrolo [2,3-c]carbazole 6, the rst nucleophilic attack at the pyrrole 2-position would give pyrrolo [3,2-c]carbazole 7, through 3-pyrrolylindole intermediates 4 and 5, respectively. Attention should also be given to the regioselectivity in the second arylation in the reaction with the benzene-type nucleophile (2 to 3).
Herein, we report a full account of our study on the direct synthesis of aryl-annulated [c]carbazoles by the regioselective gold-catalysed annulation of conjugated diynes and arenes such as benzene, pyrrole, and indole derivatives. 19 Computational investigations for elucidating the mechanism as well as redox and uorescence properties of the pyrrolo [2,3-c]carbazoles are also presented.
Using these optimised reaction conditions, we then explored the scope of the reaction. Variation of the substitution on the aryl moiety of nucleophile 8 was initially investigated (Table 2). 1,2-Dimethoxybenzene (8B) and 1,3-dimethoxybenzene (8C) served as suitable nucleophiles for gold-catalysed annulation when used as the solvent (condition B) to give benzo[c]carbazoles 3aB (quant) and 3aC (95%) in excellent yields. In these cases, the reaction using 10 equiv. of nucleophile (condition A) also permitted 70% and 40% yields of 3aB and 3aC, respectively. The reaction with benzodioxole (8D) afforded pentacyclic benzo [c]carbazole (3aD) in 76% yield. Less nucleophilic o-xylene (8E) Scheme 4 Preparation of azide-diynes. a Reactions were carried out using 1a (1 equiv.), 8A (10 equiv.), and the gold catalyst (5 mol%). b The ligand structures are shown in Fig. 2. BrettPhosAu(MeCN)SbF 6 , JohnPhosAu(MeCN)SbF 6 , and IPrAuNTf 2 were prepared in advance. The other catalysts were prepared in situ by mixing the AuCl ligand with AgNTf 2 or AgSbF 6 . c DCE ¼ 1,2-dichloroethane, TCE ¼ 1,1,2,2-tetrachloroethane. d Isolated yields. also gave the corresponding benzo[c]carbazole (3aE) in moderate yield (42%), although an increased loading of the gold catalyst (20 mol%) was required. Benzene and toluene did not provide fused carbazoles owing to their lower reactivities. In all cases using arenes 8A-E, the cascade reaction proceeded in a regioselective manner: the rst arylation occurred at the paraposition of the electron-donating substituent of 8, and the second hydroarylation occurred at the less-sterically-hindered carbon of the introduced aryl group. We next investigated the reaction using various diynes 1b-g under condition B. 21 A methyl substituent at the ortho-, meta-, or para-position of the terminal phenyl group was tolerated, producing the corresponding benzo[c]carbazoles (3bA-3dA) in good to excellent yields (70-94%). Similarly, the reaction of 1e-g bearing an electron-donating or -withdrawing group (Cl, NO 2 , or OMe) at the para-position gave the desired products 3eA-3gA (43-74% yield). The lower yield of the nitro derivative 3fA can be attributed to the less efficient coordination ability of the electron-decient alkyne(s) to the gold catalyst, which would decrease the probability of the catalyst being activated.

Reaction with pyrrole and indole derivatives
Next, we investigated the synthesis of pyrrolocarbazoles by the reaction with pyrroles 9 ( Table 3). The gold-catalysed reaction of conjugated diyne 1a with NH-pyrrole 9A produced an isomeric mixture of two annulation products 6aA and 7aA in ca. 62% yield, along with several unidentied minor products (entry 1). In this case, pyrrolo [3,2-c]carbazole 7aA was obtained as the major isomer (6aA : 7aA ¼ 25 : 75). This result can be readily understood by the more nucleophilic nature of the C2-position of NH-pyrrole than that of the C3-position. 22 Expecting that the regioselectivity of nucleophilic attack could be controlled by the steric and electronic factors of pyrrole, we subsequently evaluated the impact of substitution at the pyrrole nitrogen (entries 2-6). As expected, regioselectivity was signicantly affected by the N-substituent: N-Boc pyrrole 9F showed the highest regioselectivity to produce pyrrolo [2,3-c]carbazole 6aF (6 : 7 ¼ 92 : 8, entry 6), whereas N-benzylpyrrole 9B preferentially produced the corresponding [3,2-c]-isomer 7aB (6 : 7 ¼ 18 : 82, entry 2). The structural elucidation of 6aF and 7aF was unambiguously made by X-ray crystallographic analyses of the methylation products 6aF-Me 2 and 7aF-Me 2 (Fig. 3). The pyrrolocarbazole moiety adopted a planar geometry as expected, and the twist angle of the phenyl group was 71.1 (for 6aF-Me 2 ) and 26.2-44.0 (for 7aF-Me 2 ). 23 The larger twist angle of the phenyl group in 6aF-Me 2 was attributed to the presence of an N-methyl group in close proximity to the phenyl group.
We then optimised the reaction conditions for pyrrolocarbazole formation using diyne 1a, N-Boc-pyrrole 9F (5 equiv.),   and various gold catalysts (5 mol%) ( Table 4). Whereas Ph 3 -PAuCl/AgNTf 2 showed low reactivity (<5% yield, entry 1), other gold complexes bearing IPr, JohnPhos, XPhos, or BrettPhos as the ligand resulted in the formation of pyrrolo [2,3-c]carbazole 6aF in sufficient regioselectivities (>91 : 9) and moderate yields (55-62%, entries [2][3][4][5]. Using the most efficient ligand BrettPhos in terms of regioselectivity (6 : 7 ¼ 94 : 6, entry 5), two other silver salts were tested (AgSbF 6 and AgOTf, entries 6 and 7, respectively); however, the regioselectivity was not improved. The use of a gold complex prepared in advance slightly improved the reactivity (reaction completed within 0.5 h) and regioselectivity (6 : 7 ¼ 95 : 5, entries 8 and 9). Solvent screening and investigations of reaction temperature did not improve the yields and product ratios (see ESI †), whereas the reaction at 80 C was found to be acceptable (entry 10). From these results, we used the conditions shown in entry 8 (condition C) and entry 10 (condition D) for further studies. We subsequently investigated the scope of pyrrolo[2,3-c] carbazole formation ( Table 5). The conjugated diynes 1b-i bearing electron-donating or -withdrawing substituents on both the aryl groups reacted smoothly with pyrrole 9F to afford the corresponding carbazoles 6bF-6iF under condition C. The position of the methyl group or introduction of chloro or methoxy substituents at the terminal aryl group did not signicantly affect the reaction, and the desired annulation products were efficiently produced (6 : 7 ¼ 95 : 5). The regioselectivity was slightly decreased when using electron-decient diyne 1f substituted by a nitro group. Diynes 1h and 1i substituted by a cyano or methoxy group at the para-position to the azido group also showed relatively low selectivities (6 : 7 ¼ 81 : 19-91 : 9).
We then applied indole derivatives as the nucleophile for the annulation reaction (Table 6). The reactions of azide-diyne 1a with N-protected indoles 10A-C (R 1 ¼ Boc, Piv, or CO 2 Et) under condition C regioselectively gave the indolo [2,3-c]carbazoles 11A-C as well as several unidentied by-products. The structure of 11A was conrmed by X-ray analysis aer cleavage of the N-Boc group and dimethylation, 24 similar to the cases of 6aF and 7aF (Fig. 3). Indoles possess reactive sites other than the desired 2-and 3-positions, which may cause undesired side reactions. 16b Thus, the introduction of an electron-withdrawing group at the 5-position of indole was examined. As expected, indoles 10D-F bearing a bromo, chloro, or ethoxycarbonyl group at the 5position reacted more efficiently to afford indolo [2,3-c]carbazoles 11D-F in better yields (50-67%) under condition D.   [2,3-c]carbazole synthesis a a Reaction conditions: 9F (5 equiv.), BrettPhosAu(MeCN)SbF 6 (5 mol%), TCE, and 110 C (condition C).

Reaction mechanism
Although the nucleophilicity of arenes including heteroarenes was well investigated previously, their relative reactivity with N-Boc-pyrrole is not well understood. 25 To better understand the reaction mechanism as well as the relative reactivities of arenes employed in this study, several further experiments were performed. First, the exposure of 2aA (obtained during the reaction optimisations shown in Table 1) to the gold-catalysed reaction conditions led to its complete conversion to the corresponding benzo[c]carbazole 3aA in 90% yield (Scheme 5). Second, the reaction of 1a with pyrrole 9F was intentionally stopped before it reached completion, which afforded alkyne-substituted indoles 4aF and 5aF in 25% yield (4 : 5 ¼ 69 : 31) along with the recovered starting material. These alkynylindoles were consumed completely under gold-catalysed reaction conditions to produce pyrrolocarbazoles 6aF and 7aF in 67% and 82% yield, respectively. These results strongly indicated that the reactions proceeded through a stepwise nucleophilic attack of the arenes on the gold carbenoid followed by intramolecular hydroarylation, as per our intended reaction pathway. Competition experiments using two different arenes were then carried out (Scheme 6). The gold-catalysed reaction of 1a with anisole 8A (10 equiv.) and toluene 8F (10 equiv.) gave the anisole-derived products 2aA (30%) and 3aA (29%) along with a small amount of toluene derivative 2aF (2%) (eqn (1) in Scheme 6). Thus, the rst arylation was highly dependent on the nucleophilicity of the arene. 25 The competition between anisole 8A (5 equiv.) and N-Boc-pyrrole 9F (5 equiv.) led to the formation of the anisole-derived monocyclised product 2aA (27%) and pyrrole-derived biscyclised product 6aF (41%), the latter being the preferred product (eqn (2) in Scheme 6). This result suggested that N-Boc-pyrrole 9F was a slightly more efficient partner in the rst arylation than anisole 8A, and that anisolederived intermediate 2aA was signicantly less reactive for the second arylation than the pyrrole-derived intermediate. The competition reaction using dimethoxybenzene 8B and N-Bocpyrrole 9F gave the biscyclisation products 3aB (37%) and 6aF (34%) in comparable yields (eqn (3) in Scheme 6). This result suggested that the second arylation was accelerated by the additional methoxy group located at the para-position to the Table 6 Scope of indolo [2,3-c]carbazole synthesis a a Reaction conditions: 10 (5 equiv.) and BrettPhosAu(MeCN)SbF 6 (5 mol%). The reaction conditions employed (condition C or D) and reaction time are shown in parentheses. b Contained small amounts of impurities.

Scheme 6
Competition experiments between different nucleophiles. Condition A 0 : nucleophiles (10 equiv. each), JohnPhosAu(MeCN)SbF 6 (10 mol%), TCE, 80 C and then 140 C. Condition C/C 0 : nucleophiles (2.5 equiv. each for condition C; 5 equiv. each for condition C 0 ), BrettPhosAu(MeCN)SbF 6 (5 mol%), TCE, and 110 C. reacting carbon. 26 We then examined the kinetic isotope effect (eqn (4) in Scheme 6). The competition reaction using N-Bocpyrroles 9F (2.5 equiv.) and 9F-d 4 (2.5 equiv.) under condition C gave the corresponding pyrrolocarbazoles 6aF and 7aF, where the D/H ratios were 1 : 1 in both products. Thus, deprotonation was not the rate-determining step for the formation of these products. This result suggested that electrophilic aromatic substitution was more likely for the rst arylation than C-H insertion. 27 To further elucidate the reaction mechanism, we undertook density functional theory (DFT) calculations. The calculations were conducted at the M06L/6-31G** (for H, C, N, and P) and SDD (for Au) levels using the formation of pyrrolo [2,3-c]carbazole from 1a and N-methylpyrrole as the model reaction (Fig. 4A). As previously proposed, 16 the reaction is initiated by the intramolecular nucleophilic attack of the azide group on the activated alkyne through TS1/2 to form an indolyl-gold intermediate INT2 with a small barrier of 10.9 kcal mol À1 and a rather large endothermicity (10.5 kcal mol À1 higher than INT1). This unfavourable energy loss is compensated for by successive reaction(s). INT2 ejects nitrogen to form a gold carbenoid intermediate INT3-1 with a large stabilisation energy (45.6 kcal mol À1 ). Next, the key arylation step occurs by the intermolecular nucleophilic attack of N-methylpyrrole on the gold carbenoid INT3-2 through TS3/4, with a small barrier of 1.4 kcal mol À1 , to produce INT4. The gold rearrangement from C to N, with a reasonable barrier of 16.6 kcal mol À1 , gives an Naurated indole intermediate INT5-1. This occurs with the simultaneous re-aromatisation, protodeauration, and recomplexation of the gold catalyst with the internal acetylene, and exothermically provides the pyrrole-substituted indole intermediate INT5-2. Finally, 6-endo-dig cyclisation of INT5-2 is promoted by the gold catalyst to produce pyrrolo [2,3-c]carbazole (PD), which regenerates the active gold catalyst. The entire reaction prole is illustrated in Fig. 4B. All the transition states have reasonable energy barriers (1.4-13.1 kcal mol À1 ). The overall exothermicity is very large because of the formation of one C-N bond, two C-C bonds, and two aromatic rings. This provides the driving force for the overall reaction.

Electrochemical investigations of pyrrolo[2,3-c]carbazoles
From the viewpoint of the electronic structure, pyrrolo [2,3-c] carbazoles 6 and indolo [2,3-c]carbazoles 11 could be considered as p-fused 1,4-phenylenediamines. Thus, their cation radicals would be generated as persistent species in the p-extended form 9c-e,28 of Wurster's blue. 29 According to voltammetric analyses in CH 2 Cl 2 (Table 7), the oxidation process of pyrrolo [2,3-c] carbazole 6aF-H was irreversible as in the isomer pyrrolo [3,2-c] carbazole 7aF-H. Substitution with methyl groups at the reactive position (N-H of pyrrole) failed to stabilise the cation radical species, as shown by the irreversible oxidation wave for 6aF-Me 2 . This was despite the electron-donating nature of the substituents marginally facilitating electrochemical oxidation, as indicated by the less positive oxidation potentials. By fusing the benzene nucleus in 6aF-Me 2 to furnish the indolo [2,3-c]carbazole skeleton, the cation radical species could attain enough persistency. 11A-Me 2 underwent reversible two-stage one-electron oxidation processes, as shown by the voltammogram (Fig. 5). The redox pathway can be postulated as shown in the scheme, similar to that for 1,4phenylenediamine.
Upon the electrochemical oxidation of 11A-Me 2 in CH 2 Cl 2 , the colourless solution turned green, which demonstrated its electrochromic nature. A continuous change in ultravioletvisible-near-infrared (UV-Vis-NIR) absorption was accompanied by several isosbestic points, indicating that 11A-Me 2 was cleanly oxidised into the corresponding cation radical species (Fig. 6). Wurster's blue exhibits absorption only in the visible region (l < 700 nm). Thus, the observed red shi was induced through p-extension by the fusion of the indole rings. The carbazole skeleton gives rise to uorescence properties, 30 so the electrolysis of 11A-Me 2 also caused a change in the uorescence (FL) spectrum [l em 424, 442 (sh) nm in CH 2 Cl 2 (l ex 354 nm)]. The steady decrease in uorescence with increasing electrochemical oxidation time could be rationalised by the non-uorescent nature of its cation radical. Such dual electrochromism in which changes occur in both UV-Vis-NIR and FL spectra is rare, 30 but was also realised in our previous study on benzo [g]indolo [2,3-c]carbazole derivatives, 9c-e which were synthesised through a different mode of the gold(I)-catalysed cascade reaction. 9a Thus, the gold-catalysed synthesis of annulated carbazoles is a powerful tool for exploring the little developed category of advanced electrochromic systems.  [1][2][3][4][5]. E(Fc/Fc + ) ¼ +0.53 V under similar conditions. b Reversible redox reaction was observed.

Conclusions
We have developed a strategy for synthesising aryl-annulated [c] carbazoles through the gold-catalysed cascade cyclisation of azido-diynes. The reaction with electron-rich benzenes such as anisole and xylene gave benzo[c]carbazoles via the functionalisation of two benzene C-H bonds. The use of N-Boc-pyrrole and indoles as a coupling partner regioselectively produced their corresponding heteroaryl-annulated carbazoles, namely pyrrolo [2,3-c]carbazoles and indolo [2,3-c]carbazoles, respectively. The reaction proceeded through the intramolecular nucleophilic attack of azide on the proximal alkyne to form a gold carbenoid species, nucleophilic attack of arenes on the carbenoid, and subsequent 6-endo-dig cyclisation of the introduced arene to the other alkyne. This proposed reaction mechanism was well supported by the results of DFT calculations, competition experiments, and deuterium-labeling experiments. An N,N 0dimethylated derivative of indolo [2,3-c]carbazole showed dual UV-Vis-NIR and uorescence spectral changes on electrolysis, which demonstrates the potential utility of this reaction in materials chemistry.

Conflicts of interest
There are no conicts to declare.