Antioxidative and antimicrobial edible chitosan ﬁ lms blended with stem, leaf and seed extracts of Pistacia terebinthus for active food packaging

Methanol extracts of stem, leaf, and seed obtained from Pistacia terebinthus which are rich in phenolic compounds were used for the ﬁ rst time to produce chitosan-based antioxidative and antimicrobial ﬁ lms. All the produced ﬁ lms were characterized by FT-IR, TGA, DSC, SEM, contact angle measurements, and UV-Vis spectroscopy and mechanically. As was expected, incorporation of the plant extracts into chitosan ﬁ lms enhanced the antioxidant and antimicrobial activities e ﬀ ectively. Also, the elasticity of chitosan-seed and chitosan-stem ﬁ lms was improved. The eco-friendly nature of the produced blend ﬁ lms was determined through soil and water degradation analyses. All these ﬁ ndings lead to the conclusion that the produced blend ﬁ lms with Pistacia terebinthus extracts can be applied as alternative food packaging material.


Introduction
Petroleum-based lms are creating serious environmental problems worldwide due to their un-degradable features and harmful effects on humans and other organisms. 1,2 To escape these problems, scientists have been developing biodegradable and edible lms using natural ingredients such as lipids, proteins, polysaccharides and some other animal and plant extracts. [3][4][5][6][7] Edible lms improve the shelf life of products by delaying food spoilage, preserving food against pathogens, preventing oxidation and protecting from moisture. 3,8,9 The natural materials used for the production of edible lms have many benets such as edibility, biocompatibility, antioxidant, thermostability and antimicrobial properties, food protection, pharmaceutical applications etc. Also, these materials are inexpensive, easily available and abundant.
Chitosan, a deacetylated derivative of chitin is a well-known commonly used lm forming material. 10 Chemically chitosan consists of (1,4)-linked 2-amino-deoxy-b-D-glucan. It has a broad spectrum of applications in every practical eld of life directly or indirectly i.e. pharmacy, medicine, agriculture, food, cosmetic, textile and water treatment etc. thanks to it's acceptable mechanical, chemical and biological properties. [11][12][13] To improve the mechanical, hydrophobic, antioxidant and antimicrobial properties of native chitosan lms, various active compounds (lipids, fatty acids, essential oils and some other plant extracts) have been incorporated into chitosan lms. 14,15 P. terebinthus is a deciduous tree and belongs to Anacardiaceae family. It is a small tree growing up to 10 m in the Middle East and Mediterranean region. 16 The extracts of this plant have anti-inammation and antioxidant avonoid, omega-3 fatty acids, tannin and other valuable compounds. 17,18 Being a traditional medicine, it is using for the treatment of liver and kidney and also drinks coffee. 19,20 It can be used in the production of vegetable oils because of its high amount of oleic acid. 17 These advantages make the extracts of P. terebinthus as a suitable ingredient to be used in edible lm production.
Considering various useful properties of P. terebinthus, this study was aimed to produce edible chitosan-based lms incorporated with the stem, leaf, and seed methanol extracts. The produced lms were characterized both biologically (antimicrobial and antioxidant activities) and physicochemically (FT-IR, TGA, DSC, SEM, contact angle, mechanical properties and optical transmittance).

Extract isolation
Each plant part i.e., seed, leaf, and stem were ground to ne powder by using WARING commercial blender. Methanol was added to each sample and sonicated using WUC-D10H, DAI-HAN sonicator for 10 m. Finally, the methanol solution was evaporated and plant extracts were obtained.

Determination of phenolic compounds
The phenolic component analysis was performed on an Agilent brand 1290 Innity model HPLC instrument. Analyzes were performed using the C18 column and DAD detector according to Caponio,et al. 21 2% acetic acid (A) and methanol (B) were used as the mobile phase. The ow rate was 1 mL min À1 . For mobile phase; (3 min 95% A/5% B, 15 min 80% A/20% B, 2 min isocratic, 10 min 60% A/40% B, 10 min 50% 10 min) was applied with a gradient of 100% B. The extract was injected with 10 mL of the solution prepared with methanol to give a concentration of 20 mg mL À1 .

Film production
Four different types of chitosan lms were produced, three of which were incorporated with leaf, stem and seed extract while the fourth one was used as a control without incorporating any extract. Briey, 1 g of chitosan was added to 100 mL of distilled water and 100 mL of 1% acetic acid to make chitosan solution. The mixture was stirred for 48 h via magnetic stirrer. Then 1 g of each plant extract was added to 5 mL methanol and was vortex (VELP SCIENTIFICA, Vortex mixer) for 5 min. Then 1 mL of methanol mixture was incorporated with 60 mL of chitosan solution and 100 mL of glycerol as plasticizer. All solutions were homogenized at 26 000 rpm for 5 min with a homogenizer (Heidolph, Silent Crusher M). Each solution was poured into a plastic Petri dish. The Petri dishes were kept at 30 C for 48 h. Finally, the lms were collected from Petri dishes and stored for further analysis.

Fourier transform infrared spectroscopy (FT-IR)
The FT-IR spectra of control and blend lms were recorded at 8 cm À1 resolution and in the range between 600 to 4000 cm À1 by PerkinElmer Spectrometer.

Thermogravimetric analysis (TGA)
The thermal stabilities of the lms were carried out for both control and blend lms via TGA Q500 -TA Instruments. $5 mg of the lm sample was used for each analysis. Film samples were heated at a constant temperature rate of 10 C min À1 from 30 C to 600 C under a nitrogen atmosphere.

Differential scanning calorimeter (DSC)
To determine the endothermic and exothermic properties of lm samples incorporated with P. terebinthus extracts, DSC analysis was carried by using a Mettler Toledo DSC822e (Schwerzenbach, Switzerland). The analysis was carried out under N 2 atmosphere under the temperature range between À50 to 420 C. For each lm sample, 50 mg was used during the analysis. Film samples were placed in a hermetic aluminum pan and the heating scan was regulated as 5 C min À1 .

Scanning electron microscopy (SEM)
For determination of surface morphology and cross-section of the control lm and chitosan-based lms incorporated with P. terebinthus extracts SEM analysis was performed using Zeiss, Eco 40 (Germany). All the lm samples were coated with the layer of gold/palladium prior to taking images.

Biodegradability
Biodegradability was tested by using two different methods i.e., water solubility and soil degradation. Both of the methods were repeated thrice. Solubility in water and soil was calculated by using the following equation: WL (%) ¼ weight loss/initial weight Â 100 2.9.1. Solubility in water. For testing the lms solubility in water, 10 mg of each lm sample was cut into small pieces (2 Â 3 cm 2 ) and the initial dry weights were measured for all the four samples. Next, 20 mL of water was added to the lms and were kept at room temperature for 48 h. Aer 48 h of immersion of the lm samples, they were dried at 60 C and were weight again.
2.9.2. Soil degradability. Small pieces of the dry lms (2 Â 3 cm 2 ) were scaled and then put into the enrichment soil. About 10 mL of water was added to the samples daily and aer 15-30 days the nal weights were measured. For having the natural biodegradation environment, we did not modify soil microora. Chitosan and other nutrients in the samples were carbon and nitrogen sources for microbes and by adding water the soil microorganisms were activated.

Contact angle
Sessile drop contact angle analysis was carried out for control lm and chitosan-based lms incorporated with P. terebinthus extracts using a Data Physics video-based contact angle measurement system OCA20. Accurate drop volume was measured through soware controlled dosing volume weightdrop. The contact angle was measured by using water. For each lm sample, eight measurements were taken.

UV-Vis transparency
The transparency of control lm and chitosan-based blended with P. terebinthus plant extracts was observed in the visible light range (400-700 nm) by using a UV spectrophotometer (Shimadzu UV-3600 UV-Vis-NIR).

Mechanical properties
The tensile strength (TS), the percentage of elongation at break (EB %), and Young's modulus (YM) were recorded in the ambient temperature for control lm and chitosan-based blended lm with P. terebinthus plant extracts. The load cell of 250 N and at a rate of 5 mm min À1 , deformation of the lms were determined by using Material Testing Systems (MTS Insight 10) equipment. MTS Test Works 4 soware was applied for calculating the mechanical properties. Mitutoyo, China (a digital micrometer) was used for the measurements of the lm thickness.
2.13. Antioxidant activity 2.13.1. DPPH (2,2 0 -diphenyl-1-picrylhydrazyl) radical scavenging assay. UV-visible spectrometry was applied to evaluate the scavenging rate of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals of chitosan control and chitosan blended lms. Also, DPPH solution was used as a reagent by following a previous method reported by (ref. 14) with small amendments. Briey, 10 mg of each lm sample was dissolved in 1 mL of DPPH solution (6 Â 10 À1 M). The mixtures were incubated for 3 h in inky condition and then measured at 517 nm via a BECKMAN COULTER, DU 730 UV spectrophotometer. The analysis was repeated three times and the antioxidant activities for each sample were computed with the following formula: A sample ¼ the absorbance of the films + DPPH 2.14. Antimicrobial activity 2.14.1. Bacteria culture. The food and human-borne pathogenic microorganisms i.e., Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Proteus microbilis ATCC 14153, Proteus vulgaris ATCC13315, Pseudomonas aeruginosa ATCC27853, Enterobacter aerogenes ATCC13048, Bacillus thuringiensis, Salmonella enterica serotype typhimurium SL 1344 and Streptococcus mutans ATCC 25175 were employed to evaluate the antibacterial properties of chitosan control and chitosan lms blended with stem, leaf and seed extracts of P. terebinthus. All strains were sub-cultured on LB agar culture at 37 C for 24 h.
2.14.2. Disc diffusion test. Antimicrobial effectivity of P. terebinthus' extracts incorporated in chitosan lms against the food-borne pathogens was evaluated by using disc-diffusion method following the method reported by National Committee for Clinical Laboratory Standard (NCCLS) with minute amendments. Microorganisms' susceptibility was adjusted by 0.5 McFarland as a reference standard. Microorganism culture suspension (100 mL, 10 6 cells per mL) was swabbed onto a plate within Müeller-Hinton agar. Filter discs were placed on the Petri plate inoculated with bacteria and then, the tested samples (20 mL) were loaded on the sterile discs and incubated at 37 C for 24 h. The gentamicin discs (10 mg per disc) and solvent (alone) was used as a positive and negative control, respectively. All experiments were repeated thrice. The results were expressed as the mean diameter of inhibition zone in mm AE standard deviation (mean AE SD).
2.15. Anti-quorum sensing activity 2.15.1. Disc diffusion test. The reporter strain Chromobacterium violaceum CV026 was obtained to evaluate the anti-QS properties of chitosan lms blended with extracts of P. terebinthus. The bacterial suspensions were sub-cultured (aerobic growth, at 30 C) overnight in LB broth. Disc diffusion assay was performed to determine the QS inhibitory activity of obtained chitosan lms. Firstly, signal molecules C6-HSL (0.25 mg mL À1 ) was added to LB so-agar and then poured into the Petri plates. The strain C. violaceum CV026 was inoculated onto a surface of the solidied agar. Aer that, sterile discs were placed onto the agar and loaded with the tested samples (20 mL). Prepared plates were incubated at 30 C for 24-30 h. As a control material, only solvent (negative) and gentamicin (positive) were used. Halo formation on a purple background suggested that the tested chitosan lms exhibited the anti-QS inhibitory effect.
2.15.2. Quantitative determination of violacein inhibition. Quantitative determination of violacein inhibition by the synthesized chitosan blend lms was carried out via the ask incubation assay. LB broth inoculated with bacteria mixed with C6-HSL (5 mM) and samples were incubated for 24 h at 30 C. Violacein extraction was carried out as described by. 22 First of all, for the precipitation of violacein, the suspension from each ask (1 mL) was centrifuged at 9.000 g for 10 min. For solubilization of the violacein, the samples were dissolved in dimethyl sulfoxide (1 mL) and vortexed. The supernatant was centrifuged again and quantied at 585 nm using UV-Vis spectrophotometer (PG Instruments, T-60). The assay was done triplicate and the pigment inhibition percentage was calculated by the following formula as:

FT-IR
In order to determine the molecular interactions in the lms among chitosan and leaf, seed and stem extracts of P. terebinthus, FT-IR analysis was carried out. FTIR spectra of chitosan, P. terebinthus extracts and blend lms are shown in Fig. 1.
In the rst spectrum, characteristic chitosan peaks were recorded as -OH bonds (3288.10 cm À1 ), C-H aliphatic stretching vibration (2873.78 cm À1 ), amide I (1651.51 cm À1 ), amide II (1557.79 cm À1 ), amide III (1374.07 cm À1 ) and C-O stretching vibration (1025.51 cm À1 ). As seen from the spectrum of chitosan lm, structural changes occurred during the lm formation. Addition of the glycerol to the lm matrix (chitosan, acetic acid, and water), affected the intermolecular interactions resulted in expand in the OH bond and increase in the intensity of amide I, II and III bands. The FTIR spectra of P. terebinthus' leaf, seed, and stem extracts showed a broad absorption peak nearly 3300 cm À1 corresponding to -OH stretching. However, the spectrum of the leaf extract was observed differently from the spectra of stem and seed extracts. C-H symmetric stretching vibrations of the polyphenolic structures at around 2925.00 cm À1 showed strong absorption peaks in the seed and stem extracts, while the peak of leaf extract revealed a weak absorption in the same region. Increase in polyphenolic content in the leaf extract (see HPLC results) resulted in overlapping of the OH band with the CH stretching peak. C]O stretching vibrations were detected at 1691.76, 1698.91 and 1695.23 cm À1 for steam, leaf and seed extracts, respectively. Shiing of the C]O peak absorption to lower wavelength may result from the conjugation of the carbonyl group bonded to an aromatic ring. Conjugated C]C stretching vibrations of the aromatic ring were detected at around 1600.00 cm À1 for all the extracts. Schulz and Baranska 23 reported that the bands appearing near at 1601 cm À1 assigned to the benzene ring of the polyphenolic compounds. In the present study, these peaks of leaf and seed extract were observed at around 1606 cm À1 as more intense than the stem extract peak. This phenomenon can be explained with the leaf and seed extract had higher polyphenolic content. The peaks at around 1440.00 cm À1 can also be formed by contribution of aromatic C]C bonds. C-H out-ofplane bending vibrations of substitute aromatic ring were observed in the range of 927.00-750.00 cm À1 .
It was observed that the intensities and frequency regions of peaks in the spectrum of chitosan lm changed the type of the extract in tested samples. Inter-and intra-molecular hydrogen bonds formed between stem extract and lm matrix were resulted in broader -OH bond peak (3253.14 cm À1 ) than in the spectrum of chitosan control lm. Interestingly, C]O stretching vibration belonging to chitosan and stem extract was not observed in the chitosan-stem lm. This may be due to the fact that, all of the C]O group found in the chitosan-stem lm were affected by the hydrogen bonds. Besides, the intensities of amide II (1549.12 cm À1 ) and amide III (1379.55 cm À1 ) peaks of chitosan increased. Additionally, weak peaks observed in the wave-number range 609.00-926.27 cm À1 were attributed to C-H bending vibrations of substituted aromatic ring. This was another indication of strong intermolecular interactions between stem extract and lm matrix.
In the leaf lm spectrum, -OH bond expanded and overlapped with CH stretching peak due to the hydrogen bond formation between phenolic OH groups and chitosan. Conjugated C]C bonds of the aromatic ring were observed at 1605.44 cm À1 in the leaf spectrum. The intensities of the amide II (1532.03 cm À1 ), amide III (1322.14 cm À1 ) and CH 2 (1204.27 cm À1 ) bending absorption increased due to the effect of the molecular interactions between leaf extract and the lm matrix. Similar results were observed in the ngerprint region of the chitosan-seed lm spectrum. However, -OH bond absorption was recorded as narrower than the leaf band absorption. This may be due to the fact that the number of hydrogen bonds was less in the chitosan-seed extract lm.
Mayachiew and Devahastin 24 reported that a new peak observed at the 1720 cm À1 with the addition of gooseberry extract into the chitosan lm. They explained this peak as an ester linkage formed between chitosan and polyphenolic compounds. In the present study, the new peaks were observed at around 1720 cm À1 with the increase in the polyphenolic contents of extract. This result was an agreement with the Mayachiew and Devahastin result. The FTIR analysis results revealed that successfully incorporation of different extracts of P. terebinthus into the chitosan lm resulted in different molecular interactions in the lm matrix.

TGA analysis
The thermogravimetric results of the chitosan control and blend lms are presented in Fig. 2. For all the lms, degradation was recorded in three steps. The rst stage was recorded around 50-100 C (about 5% decomposition for each lm). This rst mass loss can be attributed to the water evaporation from the polymeric structures. The second step was observed around 120-200 C and it can correspond to the decomposition of glycerol. 25 The main mass loss was recorded around 240-300 C and it was because of the denaturation of chitosan polymeric organization and plant extracts. 26 In this step chitosan-stem extract lm showed similar stability to the control lm, however, the gure demonstrates, incorporation of seed extract to chitosan matrix decreased the thermal stability. These stages of decomposition were in agreement with the chitosan-based lm incorporated with thyme oil. 27 At the end of thermal degradation, ash contents for chitosan control, chitosan-stem, chitosan-leaf and chitosan-seed lms was recorded as 34.44%, Fig. 1 FT-IR spectra of chitosan; stem, leaf and seed extracts of Pistacia terebinthus; chitosan control film; chitosan-stem film; chitosanleaf film and chitosan-seed film.
29.19%, 39.122 and 35.51%, respectively. In general, the thermal stabilities of chitosan blend lms were not affected by the incorporation of plant extracts.
Additionally considering some previous reports regarding the production of chitosan blend lms, same phenomena was recorded in those reports; i.e. Pelissari, F.M. et al., 28 observed the same results when cassava starchÀchitosan lms were incorporated with oregano essential oil. The overall thermal stability did not reveal any increase in case of blend lms. Kanmani P. et al., 29 reported that no alteration was observed in the thermal stability (dTGA max ) of the chitosan lms when blended with carrageenan/grapefruit seed extract.

DSC
The DSC thermograms of the chitosan lm and composite lms are shown in Fig. 3. Chitosan-control lm exhibited two endothermic (72.24 and 154.64 C) and one exothermic peak (269.65 C). The rst exothermic peak at 269 C corresponded to the phase inversion temperature of decomposition of chitosan. Peng, Wu and Li 30 reported the similar result for chitosan lm. Also, they explained that the phase inversion temperature was reduced by the addition of tea extract into the chitosan lm. The similar results were found in the present study. The thermal stability of chitosan lms decreased with the incorporation of the leaf, stem and seed extracts, due to the decreasing of crystallinity of the lms Martins J.T. et al. 31 Blend lms incorporated with P. terebinthus extracts showed two endothermic peaks. For chitosan-stem, chitosan-leaf and chitosan-seed the two endothermic peaks were observed as; 80. .44 C, 88.01-186.66 C and 102.45-203.15 C, respectively. The rst endothermic peaks for chitosan-control and blend lms can be attributed to evaporation of solvent traces (water, acetic acid, and methanol) used during the production of composite lms. 27 While the second endothermic DSC peaks for blend lms can be ascribed to the denaturation of glycerol and extract components.

SEM
The surface characteristics of chitosan control and chitosan blend lms are presented in Fig. 4. As is seen from the gure, surface morphology of chitosan-control was observed as smooth, compact, and without any crack or pores ( Fig. 4a and  b), it was similar to results of the other studies. 25,27 Nevertheless, composite lms exhibited irregular forms. The chitosan-stem extract lm had many bulges and tiny particles on the surface ( Fig. 4c and d). Chitosan-leaf extract lm also exhibited heterogeneous form, but it had lower bulges and higher pieces ( Fig. 4e and f) and was more homogenous than chitosan-stem extract lm. Although chitosan-seed extract lm showed some roughness and particles, it was detected as a more regular lm to the blend lms ( Fig. 4g and h). Almost similar surface morphologies were revealed from the SEM micrographs in other studies reporting the incorporation of fruit extracts in chitosan blend lms. 32-35

Water solubility and soil degradation
The water solubility of the chitosan-control lm was recorded as 24% in 48 h. Incorporations of plant extracts into the lms demonstrated signicant (P < 0.05) increase in lm solubilities as 35.27%, 45.31% and 50.7% for chitosan-leaf, chitosan-stem,  and chitosan-seed lm, respectively. This chitosan lm degradation was witnessed in accordance with previous reports (23.52 AE 1.26%). 36 The weak intermolecular interactions between plant extracts and chitosan matrix resulted in higher degradation values into the chitosan blend lms. Higher solubility rate for the chitosan-seed lm was probably due to the faster diffusion and disruption of molecular interactions.
Soil degradation rates in 15 days were recorded as 89.04% for chitosan control lm, 98.76% for chitosan-leaf lm, 78.65% for chitosan-stem lm and 56.35% for the chitosan-seed lm. The chitosan-leaf lm exhibited higher soil degradability, however, lower soil degradability was observed for the chitosan-seed lm.

Contact angle
The contact angle is another parameter to determine hydrophobic and hydrophilic nature of materials. Water wettability of the lm surface is associated with the nal angle of the water drop. In order to check the effect of the stem, seed and leaf extract on lm wettability, the contact angle values were calculated for chitosan control, chitosan-stem, chitosan-seed and chitosan-leaf lms as 86.3 AE 1.43 , 82.1 AE 1.83 and 91.35 AE 3.05 and 61.8 AE 5.54 , respectively. Chitosan-leaf extract lm showed hydrophilic feature as its contact angle observed at 61.8 . However, chitosan-leaf extract lm has lower water solubility rate. As seen from the HPLC results, leaf extract has maximum polyphenolic content. Increase in polyphenolic content caused strong interactions in the lm matrix and reduced water solubility. However, hydrophilic groups of the polyphenolic compounds such as carboxyl, hydroxyl were interacted with the water on the lm surface and reduced the contact angle. Same phenomena were reported by Jin et al., 37 when chitosan matrix was incorporated with genipin. An increase in the genipin content affected the overall lm wettability in a positive manner. In another report by Ramírez et al., 38 a reducing pattern was observed for contact angle of the chitosan-based when incorporated with murta leaves extract.

Visual appearance and light transmission of the lms
Appearance and color of the food coatings are one of the most important factors inuencing the nutrients quality and customers attraction. As is shown in the Fig. 5 chitosan control lm was appeared as clear, glossy, smooth, and without any tents. The visual appearance of chitosan-stem extract, chitosanleaf extract, and chitosan-seed extract composite lms was observed as light yellow, green and light brown color, respectively. Previous literature reports also, explained the same phenomena, that adding plant extracts to chitosan matrix, changed the lm's color. 27,39 Control, chitosan-stem extract, and chitosan-seed extract lms were homogeneous and without any wrinkle, pores or bubbles. Contrarily, chitosan-leaf extract lm exhibited granules and evenly distributed rare bubbles on its  surface. Chitosan-leaf lm had not enough exibility and it was broken easily. The color of chitosan-leaf extract lm can be due to different compounds in the leaf structure and existence more pigments (chloroplasts) than the other parts.
Optical transmittance of chitosan control and blend lms was measured in the visible spectrum (Fig. 6). The addition of plant extracts into chitosan lm decreased the transparency. The transmittance rate at 700 nm was recorded 89.79% for chitosan control lm, 66.6% for chitosan-seed lm, 63.16% for chitosan-stem lm and 44.76% for the chitosan-leaf lm. The gradual decrease in the transparency rate of blend lms can possibly due to the presence of pigments and tints on the lm surface.

Mechanical properties and thickness
Evaluation of tensile strength, Young's modulus and elongation at break are vital characteristics for understanding the behavior of biomaterials during their practical applications i.e., packaging and shipping. 40 In this context, mechanical properties of blend lms were measured and the results are given in Fig. 7. Young's modulus can be described as a measurement of the stiffness of any bio-based materials. On the other hand, the tensile strength can be explained as the maximum engineering stress level reached in a tension test. When the value of Young's modulus is large, it indicates the stiffness of the material. In this context, in the present study, due to the higher value of Young's modulus for chitosan control and chitosan-leaf lms were observed comparatively stiffer. For chitosan control lm, Young's modulus (1251.89 AE 33.90 MPa) and tensile strength (26.86 AE 3.50 MPa) were recorded about two times higher than chitosan-leaf extract lm and much higher than chitosan-stem extract and chitosan-seed extract lms. In this context, chitosan control and chitosan-leaf lms were recorded stiffer than other lms.
From Fig. 7, it can be seen that chitosan-stem extract and chitosan-seed extract lms exhibited a large elongation at break. Therefore, these composite lms had ductile nature. On the other hand, elongation at break values of chitosan-stem extract (72.13 AE 1.65%) and chitosan-seed extract (79.62 AE 4.86%) lms was observed much higher than chitosan control lm (3.72 AE 0.57%) and chitosan-leaf extract lm (3.11 AE 1.23%). These results exhibited that incorporation of seed and stem extract from P. terebinthus highly increased the elasticity of the chitosan lm. So the chitosan control lm can be suggested as the stiffest among the other considered lms. It can be noted that tensile strength and Young's modulus of chitosan control lm were recorded about two times higher than chitosan-leaf extract lm and much higher than chitosan-stem extract and chitosan-seed extract lms. It is evident that the chitosan-stem extract exhibited exible and weak behavior as indicated by a very high elongation at break, a very low tensile strength (3.28 AE 0.15 MPa). These results also revealed that incorporation of stem and seed extract from P. terebinthus highly increased the elasticity of the chitosan lm.
When examined visually and handled, it was realized that chitosan-leaf extract lm was fragile. However, chitosan-stem extract and chitosan-seed extract lms were exible. In some earlier studies, the addition of essential oils and plant extracts into the chitosan lms increased the elongation at break 39,41,42 as it was recorded in the present. Park and Zhao 43 reported the reduction in tensile strength and elongation at break due to the decreasing of the crystallinity of the edible lm and intermolecular hydrogen bonding formed in the lm matrix. As seen from the HPLC results, the polyphenolic content of the leaf extract was too high, which capable of the H bonding.

Phenolic compounds
Phenolic compounds of stem, leaf and seed extracts from P. terebinthus were determined and the results are presented in Fig. 6 Optical transmittance of the chitosan control film and blend films incorporated with Pistacia terebinthus' extracts as; chitosan-stem film, chitosan-leaf film and chitosan-seed film. Fig. 7 The mechanical properties of the chitosan control film and blend films incorporated with Pistacia terebinthus' extracts as; chitosan-stem film, chitosan-leaf film and chitosan-seed film.  (Fig. 8). Major phenolic compounds of leaf extract were recorded as epicatechin, sinapic acid, gallic acid and benzoic acid. Main components of stem extract were observed as hydroxybenzoic acid and gallic acid. In seed extract, gallic acid, epicatechin, and catechin were found as main phenolic compounds.

Antioxidant activity
DPPH assay was used to determine the radical scavenging activities of the produced chitosan lms incorporated with stem, leaf and seed extracts of P. terebinthus along with chitosan control. Antioxidant activities of chitosan control lm, chitosanstem lm, chitosan-leaf lm and chitosan-seed lm were measured as 37.21 AE 2.07%, 94.43 AE 0.13%, 93.98 AE 0.17% and 95.91 AE 0.15% respectively. As is seen, the addition of methanol extracts from P. terebinthus' stem, leaf and seed notably improved the antioxidant properties of the blend lms. This can be ascribed to already known the high antioxidant activity of P. terebinthus due to its hydrogen-donating property and rich phenolic content. 17 Considering these results, it can be stated that with current analysis, one of the main aims of the present study was achieved as highly antioxidative edible lms were produced by successfully incorporating the P. terebinthus extracts. Current antioxidant results for the produced lms were observed to be higher than previously reported chitosan lm with green tea extract 14 and was found close to chitosan-based lm with thyme oil. 27

Antimicrobial efficacy
The antimicrobial activities of chitosan control, chitosan blend lms with three different extracts of P. terebinthus was investigated against food borne pathogens. This is the rst report on the antimicrobial activities of P. terebinthus extracts blended chitosan lms against food pathogens. The chitosan blend lms with the plant extract were found to be effective against especially Gram-negative bacteria such as P. microbilis, P. vulgaris, P. aeruginosa and E. coli (inhibition zones were ranged in 28.19 AE 0.92-21.33 AE 0.74 mm). Gram-negative bacteria are oen resistant to conventional antimicrobial drugs because of their tenacious nature ability and expression of active efflux pumps however in the current study, Gram-negative bacteria did not reveal any activity in the case of chitosan blended lms with P. terebinthus' extracts. 44,45 Furthermore, different compounds of P. terebinthus incorporated into chitosan lms also presented antibacterial zones as 26.63 AE 0.81-20.03 AE 0.68 mm against Gram positives. Also, chitosan-seed lm indicated the greatest antimicrobial activity (ranged in 28.19 AE 0.98-20.33 AE 0.72 mm) than the other blended lms. In all tests, control disc with the only solvent displayed no inhibition zone and chitosan control lm showed lower inhibition zone than blended lms. From this result, it was concluded that incorporating of the plant extract into chitosan lms provided higher antimicrobial activity because of compounds of P. terebinthus contain several volatile ingredients, most important of which are avonoids and polyphenols. 46,47 In addition, the inhibition zones of chitosan blend lms indicated higher activity than that  of commercial antibiotic (gentamicin). To the most important goal of our study was that to incorporate the plant extract which is rich in avonoids compounds into the chitosan matrix which has antimicrobial property can efficiently eliminate the persistent microorganisms such as S. typhimurium, E. coli, B. thuringiensis, P. aeruginosa and P. vulgaris. The discovery of plant and biopolymer based nontoxic agents could promote antimicrobial activity with minimal drawbacks since enhanced toxicity due to the application of conventional toxic chemicals.

QSI bioassay
In the current study, chitosan lms blended with extracts of stem, leaf, and seed from P. terebinthus was assessed for the anti-QS activity against the reporter strain C. violaceum CV026.
In the disc diffusion method, the tested chitosan lms displayed signicant values of anti-QS activity which was computed according to the loss of purple pigmentation. All synthesized lms inhibited the production of violacein with zones ranged in 8.11 and 29.38 mm. According to radios of inhibition zones, chitosan blend lms with stem, leaf and seed extracts of P. terebinthus displayed higher pigment clearance than chitosan control lm. Furthermore, interestingly the pigment inhibition of chitosan blended lms, especially with seed, showed greater activity than commercial antibiotic (gentamicin). The highest values of quorum-sensing activity were obtained from chitosan-seed lm.
On the other hand, quantitative determination of the violacein production inhibition was also evaluated. All the tested chitosan blend lms exhibited a signicant reduction in production of violacein with the ranged in 28.13 AE 2.4-89.34 AE 3.5% (Fig. 9). Similarly, with results of QS inhibition zone, chitosan-seed and chitosan-stem lms exerted a higher pigment inhibition (89 AE 3.6 and 81 AE 3.5%, respectively) against C. violaceum CV026 than chitosan-leaf lm (72 AE 3.3%).

Conclusion
For the rst time, stem, leaf and seed extracts obtained from P. terebinthus containing high phenolic substances were transferred into chitosan lms. As a result, high antioxidative and antimicrobial lms (against nine food-borne pathogens) were successfully produced and well characterized with commonly used analytical techniques. Additionally, supplementing plant extracts into chitosan lm enhanced the overall elasticity of chitosan-stem and chitosan-seed lms. Current results showed that the produced blend lms could be suggested as a suitable alternative to synthetic plastics for applications in food coating and packaging industry.