Nickel(ii)-promoted specific hydrolysis of zinc finger proteins.

In this work we demonstrate that the previously described reaction of sequence specific Ni(ii)-dependent hydrolytic peptide bond cleavage can be performed in complex metalloprotein molecules, such as the Cys2His2 zinc finger proteins. The cleavage within a zinc finger unit possessing a (Ser/Thr)-X-His sequence is not hindered by the presence of the Zn(ii) ions. It results in loss of the Zn(ii) ion, oxidation of the SH groups and thus, in a collapse of the functional structure. We show that such natural Ni(ii)-cleavage sites in zinc finger domains can be edited out without compromising the DNA binding specificity. Inserting a Ni(ii)-susceptible sequence between the edited zinc finger and an affinity tag allows for removal of the latter sequence by Ni(ii) ions after the protein purification. We have shown that this reaction can be executed even when a metal ion binding N-terminal His-tag is present. The cleavage product maintains the native zinc finger structure involving Zn(ii) ions. Mass spectra revealed that a Ni(ii) ion remains coordinated to the hydrolyzed protein product through the N-terminal (Ser/Thr)-X-His tripeptide segment. The fact that the Ni(ii)-dependent protein hydrolysis is influenced by the Ni(ii) concentration, pH and temperature of the reaction provides a platform for novel regulated DNA effector design.


Introduction
Transcription factors of the Cys 2 His 2 zinc finger (ZF) family control gene functions in living organisms by binding to specific nucleic acid sequences. 1,2 The canonical sequence of a finger unit, able to form a bba secondary structure with a tetrahedral metal ion binding site, was defined on the basis of a library of native sequences as (Tyr,Phe)-X-Cys-X 2,4 -Cys-X 3 -Phe-X 5 -Leu-X 2 -His-X 3-5 -His (Zn(II) binding residues underlined). 3 ZF proteins possess a modular assembly in which each ZF unit recognizes three subsequent nucleotides. 1,2 This allows for targeting DNA sequences of a 9-12 base pair length by a cascade of such units in a ZF protein. [4][5][6][7][8][9] Artificial ZF proteins with desired DNA specificities can be obtained by altering the amino acids in the a-helical DNA base recognition region according to the well-known methodologies. [10][11][12][13][14] A popular approach is to utilize effector molecules (i.e. ligand or metalbinding regulatory sequences or an endonuclease catalytic domain) linked to ZF proteins, thereby providing an opportunity to generate artificial transcription factors or enzymes for specific DNA actions. 2,[15][16][17][18][19][20][21][22][23][24][25] Previously we reported on Ni(II)-induced peptide bond hydrolysis that occurs at the N-terminal side of Ser or Thr residues in peptides, [26][27][28][29][30][31][32][33][34][35][36] designed recombinant proteins 37,38 and flexible regions of native proteins 37,39 bearing X-(Ser/Thr)-X-His-X sequences (X being any amino acid except for Cys or Pro before or after Ser/Thr). The method found application in the affinity tag removal from fusion proteins by Ni(II) ions. 37,38 The cleavage is site specific and sequence selective making this method unique among other approaches using metal ions. 31,37,40 The molecular mechanism of this reaction was proposed earlier 31,35 and is depicted in Fig. S1 (ESI †). The formation of a square planar Ni(II) complex is strictly required for the reaction: it contains the Ni(II) ion bound to the imidazole nitrogen of the His residue and three preceding amide nitrogens. 31,35 Various human transcription factors contain potential Ni(II) cleavage sites within their Cys 2 His 2 ZF units. The 27 amino acid long peptide, representing the 3 rd ZF unit of the Sp1 human transcription factor was shown to be cleaved by Ni(II) ions even though the His residue crucial for the reaction was initially complexed to the structural Zn(II) ion. 32 The Zn(II) binding site and the structure of the ZF unit was abolished in the reaction. Naturally occurring Ni(II)-cleavage sites, which are present in nearly 25% of human Cys 2 His 2 ZF sequences 32 can thus be considered potentially susceptible to hydrolysis upon the exposure to Ni(II) ions naturally present in human tissues. 41 Nickel is absorbed through the diet, skin, lungs and then taken up by cells. 42,43 By analogy with the hydrolytic cleavage of histone H2A observed in Ni(II)-exposed cell cultures, endogenous nickel present in human cells may damage ZFs as part of its toxicity.
The knowledge, summarized above, inspired us to challenge sequence specific Ni(II)-induced peptide bond cleavage within a complex metalloprotein such as a Cys 2 His 2 -type classical ZF array consisting of three ZF units and also possessing a Ni(II)-cleavable N-terminal deka-His fusion tag. Our aim was to verify that the binding of Zn(II) ions is unaffected by the presence of the Ni(II) ions in the uncleaved finger units, i.e. they are not replaced by Ni(II) ions and the oxidation of cysteines in the binding sites is not catalysed by Ni(II) ions. We analysed and optimized the cleavage of two ZF proteins by Ni(II) ions at a single peptide bond between the protein and the affinity tag, and investigated the structural integrity, as well as the Zn(II) and DNA binding properties of the products by spectrometric and electrophoretic methods.

Gene construction
Two ZF proteins, denoted by P-1MEY and P-1MEY# were investigated, both consisting of three ZF units. The P-1MEY protein is similar to that included in the ZF protein/DNA crystal structure, with 1MEY PDB code, 44 and the P-1MEY# ZF protein is its modified version as described below. The gene of the P-1MEY protein was ordered as a full length DNA with a stop codon (IDT Japan Ltd) and ligated into a pET-16b vector between the NdeI and BamHI sites. The gene of the P-1MEY# protein was constructed starting from the above DNA sequence by the modified QuikChanget site-directed mutagenesis method (QCM, Stratagene, La Jolla, CA) using the following primers: 1MEY#-F (5 0 -tcgccatcagcgtacccatac-3 0 ) and 1MEY#-R (5 0 -accaggtt atcgctgcgggaaaatgacttc-3 0 ) according to Scheme S1 (ESI †). Consequently, a His was replaced with Asn (H99N) and a Ser with Val (S101V). After the amplification of the DNA with KOD-FX DNA polymerase (TOYOBO, Japan), the resulted DNA fragments were treated by T4 polynucleotide kinase (TOYOBO, Japan) and T4 ligase in the ligation high premix (TOYOBO, Japan). DH5a cells were transformed with the resulted plasmid, and after the DNA purification the success of the mutagenesis was verified by a standard sequencing procedure.

Protein expression and purification
The P-1MEY and P-1MEY# proteins were expressed in BL21 (DE3) E. coli cells, which were cultured to OD of ca. 0.3, then the protein expression was induced by 0.2 mM IPTG at 20 1C for 18 hours. Cells were collected and resuspended in a buffer containing 500 mM NaCl, 100 mM HEPES (pH 8.2), and 10 mM imidazole, and then sonicated. The pET-16b-P-1MEY and pET-16b-P-1MEY# plasmids permitted introduction of the N-terminal deka-His tag and thus, the expressed proteins were subjected to Ni(II)-affinity chromatography as the main purification step. Applying an imidazole concentration gradient in the eluent, most of the target proteins were obtained at 300 mM imidazole as shown in Fig. S2a (ESI †). The Zn(II) load of the ZF proteins was verified by EDTA titration of the ZF proteins monitored by circular dichroism spectroscopy (Fig. S2b, ESI †). The Zn(II) contents of the purified ZF proteins were 3.0 AE 0.2 equivalents/protein molecule.

Protein hydrolysis by Ni(II) ions
30-40 mM P-1MEY or P-1MEY# proteins were incubated with 400 mM Ni(II) (B10-fold molar excess) in a 100 mM HEPES buffer with such an addition of Zn(II) that a 10-100 mM final concentration of Zn(II) ions in excess of the protein remained in solution. The conditions of the hydrolytic experiments varied between 50 1C, pH 8.2 and 37 1C, pH 7.4 (i.e. close to physiological conditions). Control reaction mixtures without Ni(II) were incubated for 72 hours under the same conditions. All samples were prepared and then incubated in low adsorption 1.5 ml tubes (LowBind, Eppendorf), using a thermoblock (TB-941U, JW Electronic). 20 ml samples were collected from reaction mixtures at given time points and the reactions were terminated by acidification (2% TFA), as performed and justified before. 35 Samples were analysed by polyacrylamide gel-electrophoresis (Tricine-SDS-PAGE 45 ), and in parallel by HPLC (Breeze, Waters) using an ACE C18 analytical column. The eluting solvent A was 0.1% TFA/water and solvent B was 0.1% TFA/90% acetonitrile.

Electrospray ionization mass spectrometry (ESI-MS)
The samples investigated by ESI-MS (Premier, Q-Tof1, Waters) were either obtained from the HPLC separation of the cleavage products as described above (P-1MEY), or taken directly from the reaction mixture (pH 8.2) with or without the sample acidification to pH o4.0 (P-1MEY#). The samples were analysed following a buffer exchange (to 100 mM NH 4 HCO 3 , pH 8.1) or desalting on a RP-C18 pre-column (Waters).

Circular dichroism (CD) measurements
CD measurements were performed on a JASCO J-815 spectropolarimeter in quartz cuvettes (d = 2 mm or 0.1 mm). The spectra were recorded in the 180-300 nm range, using 0.5 nm resolution, 2 s response time, and scanning speed of 20 nm min À1 . The samples were taken directly from the hydrolytic reaction mixture at the beginning and after the termination of the reaction. The solvent was exchanged to 5 mM phosphate buffer (pH 7.4) prior to the CD experiment using an Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-3 membrane having a nominal molecular weight limit of 3 kDa.

Electrophoretic mobility shift assay (EMSA)
The 1MEY+2bp oligonucleotide probe of the sequence 5 0 -CG TTCTGCCTCACTTTTGTGAGGCAGAACG-3 0 , which included the 1MEY target sequence (underlined) and the ZFP#2+2bp probe of the sequence 5 0 -CGGGGATAGGCACTTTTGTGCCTATCCCCG-3 0 were used for EMSA experiments. These oligonucleotides were labeled with [g-32 P]ATP by T4 polynucleotide kinase (TOYOBO). The labeled oligonucleotides were self-annealed, to yield the double strand sequence stabilized by the TTTT loop, and then purified from native PAGE, and used as probes. EMSA experiments were performed in 10 ml of a 60 mM HEPES buffer, pH 8.2, containing 10% (V/V) glycerol, 45 mM NaCl, 32 P-end-labeled probe (15 fmol) and P-1MEY or P-1MEY# protein at a concentration giving rise to a protein excess between 0 and 100 fold, as indicated in Fig. 1. The reaction mixtures were separated on a 6% (m/V) polyacrylamide gel containing 12.5 mM Tris buffer and 96 mM glycine. Radiolabeled probes were visualized by a BAS2000 imager. Further EMSA experiments were carried out with a DNA probe including four tandem copies (underlined) of the favourable 1MEY binding sequence. It was constructed by the annealing and subsequent prolongation of the following deoxy-oligonucletide primers to yield a 72 bp DNA: 5 0 -GGCGAATTCGAGGCAGAATGCTTATTCTGCCTCGAGCTCG AG-3 0 5 0 -GGCGAATTCGAGGCAGAATAAGCATTCTGCCTCGAGCTCG AG-3 0 . The DNA samples (c final = 4.0 mM) were incubated with increasing amounts of the Ni(II)-cleaved P-1MEY# ZF protein from 1 : 1 to 1 : 4 molar ratio at pH 7.4 in a 100 mM HEPES buffer.
The reaction mixtures were separated on a 2% (m/V) agarose gel and the DNA was visualized by the intercalated ethidium ion fluorescence using an UviDoc gel documentation system.

Results and discussion
Choice and redesign of the ZF protein The P-1MEY ZF protein sequence is based on the consensus sequence CP-1 derived by Berg et al., 46 and confirmed by a recent study, 47 with the exception of the amino acids responsible for DNA recognition. It is a well studied protein suitable for our model cleavage experiments. The sequence was derived from a ZF protein (denoted as P-1MEY-PDB) previously structurally characterized in the complex with its cognate DNA (PDB code 1MEY). 44 The amino acid sequences of the P-1MEY and mutant P-1MEY# proteins investigated by us are aligned to the P-1MEY-PDB protein in Scheme 1. The core sequence of P-1MEY responsible for the functional structure and DNA recognition was kept intact. The sequence was modified here by the deka-His tag at the N-terminus for the purpose of affinity purification. Further amino acids, following the affinity tag sequence were encoded by the pET-16b plasmid: SSGH contains the underlined Ni(II)-sensitive sequence and IEGR is the Factor Xa protease (NEB) recognition sequence. Short extensions (additional amino acids compared to the P-1MEY-PDB) at both termini of the P-1MEY protein sequence (see Scheme 1) can be derived from the consensus sequence as they are found in the Zinc Finger Tools program used for protein redesign (vide infra). 13 P-1MEY contains three X(S/T)XHX sequence motifs that can undergo hydrolytic cleavage upon Ni(II)-binding. Two of them are located close to each other within the DNA recognition region of the 3 rd ZF unit (similarly to the 3 rd ZF unit of Sp1 -Scheme 1), while the third sequence is located between the N-terminal deka-His tag and the ZF protein sequence. Based on the experiments with the Sp1 C-terminal peptide 32 the possible Ni(II)-dependent hydrolysis within the 3 rd ZF unit of P-1MEY may be expected to affect the properties of this finger. Therefore, we redesigned P-1MEY ZF protein into P-1MEY# with a single Ni(II)-sensitive sequence at the N-terminus, by editing out the hydrolysable sequences in the 3 rd finger.
Since the necessary substitutions fall within the DNA recognition sequence, the Zinc Finger Tools program 13 was utilized to maintain the DNA binding specificity. For the target DNA sequence derived from the 1MEY/DNA structure, 44 the program yielded a ZF protein sequence differing at various sites from the original one (Zinc Finger Tools sequence in Scheme 1). To obtain the P-1MEY# protein we introduced only the minimal changes limited to the 3 rd ZF unit involving the His to Asn (H99N) and Ser to Val (S101V) substitutions (Scheme 1; see the Materials and methods section for details).
The DNA-binding specificity was maintained in the redesigned ZF protein The DNA binding properties of the P-1MEY-PDB ZF protein were previously studied and the 5 0 -GAGGCAGAA-3 0 DNA sequence included in the 1MEY crystal structure was considered as a favourable binding site of the P-1MEY protein. 44 After the expression and purification procedures the DNA binding specificity of both P-1MEY and the modified P-1MEY# ZF proteins was verified by electrophoretic mobility shift assays (EMSA) using various short DNA molecules. Fig. 1 shows that the modifications at the two termini did not affect the DNA binding specificity of the P-1MEY protein, and that the P-1MEY# mutant has recognition properties comparable to the P-1MEY protein. P-1MEY# preferred to bind the target DNA similarly to the original protein (see also Fig. S3, ESI †). Besides, P-1MEY# was less prone to form non-specific complexes at a 100-fold protein excess with the ZFP#2+2 bp non-specific DNA probe, while under the same conditions the addition of P-1MEY resulted in a shifted DNA band characteristic for this non-specific DNA interaction. A small amount of such non-specific complex between the P-1MEY protein and the 1MEY+2 bp specific DNA probe was observed only at a high protein excess.

Monitoring the Ni(II)-induced hydrolysis of the ZF proteins
We verified the Ni(II)-cleavage for all susceptible sequences: three in P-1MEY (SGH, SDH, SRH) and one in P-1MEY# protein (SGH). The hydrolysis reactions were monitored by Tricine-SDS-PAGE and products were analysed by HPLC.
The imidazole groups of the N-terminal deka-His tag have the ability to complex Ni(II) ions and compete in this respect with the His residue from the hydrolytic sequence X-(Ser/Thr)-X-His-X. To overcome this effect we used at least a 10-fold molar excess of Ni(II), as demonstrated before to be effective for protein cleavage. [37][38][39] The hydrolysis removes the His-tag, while the catalytic Ni(II) ion remains coordinated to the (Ser/Thr)-X-His motif of the protein (C-terminal product).
The CP-1 ZF peptide showed a high affinity towards Zn(II) ions (K d B 2 pM), 46 and a recent re-investigation of ZF affinities for Zn(II) ions indicated even lower K d values. 48 Accordingly, P-1MEY and P-1MEY# proteins were purified in their zincloaded forms. Nevertheless, we used an additional amount of Zn(II) ions (final excess: 10-100 mM) in each reaction mixture to decrease the possibility of the Zn(II)/Ni(II) substitution in ZF domains during the reaction. Metal ion substitution was suggested to change the specificity of DNA recognition due to alteration of the ZF structure, resulting from different coordination geometries of these two metal ions. 49 Change of the protein structure was also supported in the presence of other metal ions, such as Cd(II) and Hg(II), 50 and the ZF cleavage was initiated by a dinuclear Pt(II) complex initially replacing the Zn(II) ions from the thiolate sites. 51 The structural integrity of P-1MEY and P-1MEY# proteins in the presence and absence of Ni(II) ions was verified by CD spectroscopy upon iterative heating and cooling (from 10 1C to 50 1C and back) of solutions adequate for the hydrolytic experiments. The collapse of the structure of ZF proteins at high temperatures would result in a disordered structure and thus, in an altered spectral pattern, similar to that obtained for the apo-protein (Fig. S2b, ESI †). It has to be noted here that this phenomenon was observed upon addition of Cu(II) ions (unpublished results), but it was excluded for Ni(II), as no significant changes were detected in the presence of the latter metal ion in the intensity of the CD signal, exemplified by the P-1MEY protein in Fig. S4 (ESI †).
Typically, three bands were observed in SDS PAGE after the hydrolysis of the P-1MEY protein in the 10-15 kDa range, corresponding to the substrate and the protein cleavage products, and a further one appeared in the low molecular weight range, corresponding to the short terminal peptide fragments (Fig. S5, ESI †). The presence of distinct bands suggested that specific cleavages occurred at least at two of three potential Ni(II) cleavage sites. The sequence redesign process allowed P-1MEY# to be converted into a single high molecular weight hydrolysis product (Fig. 2).
According to the kinetic analysis, the hydrolysis of the P-1MEY# mutant protein occurred more slowly than that of the P-1MEY (t 1/2 = 30 h vs. 14 h at 37 1C and pH 8.2). The control experiment without the Ni(II) ions revealed only a marginal degradation of the protein after 72 h of incubation at the elevated temperature and pH.
The Ni(II)-induced hydrolysis of P-1MEY and P-1MEY# ZFs was investigated at 50 or 37 1C and pH 8.2 or 7.4 to demonstrate the significant effect of the temperature and pH on the reaction rate. The hydrolysis experiments repeated under physiologicallike conditions at 37 1C and pH 7.4 indicated that the reactivity pattern was maintained, although the reaction rate decreased significantly for both P-1MEY and P-1MEY#. The densitometric results obtained from SDS PAGE gel analysis are shown in Fig. S6 (ESI †). These data were used to calculate reaction rate constants. The comparison of t 1/2 values in the 1 st order kinetic approximation revealed their strong pH dependence. We observed a 9-fold increase of the reaction rate between pH 7.4 and 8.2 at 37 1C (t 1/2 of B11 d, and B 30 h, respectively). The increase of the temperature from 37 1C to 50 1C resulted in a further acceleration of the reaction rate (t 1/2 B 7.5 h). The pH dependence of the 1 st order rate constant for the Ni(II)-assisted hydrolysis reaction has a sigmoidal shape. The reaction rate is maximal at pH above pH 8.2, where the square planar (4N) complex predominates. At lower pH (pH 7.4) the extent of the formation of the active 4N complex is much lower: 20-80 times less, compared to pH 8.2. 31,52,53 Verification of the cleavage specificity and binding of the metal ions by MS The resolution of the gel electrophoresis is not sufficient to precisely identify the hydrolytic cleavage sites. Therefore, we analysed the reaction mixtures by ESI-MS. The experimentally determined masses of the substrate proteins and their Ni(II)-hydrolysis products agreed well with the theoretical predictions. Among the P-1MEY products we identified short GHHHHHHHHHHS and SRHQRTHTGKKTS N-and C-terminal peptides, respectively. The peptide resulting from the cleavage at the SDH site, SDHLSRHQRTHTGKKTS, was not detected as the cleavage within the SRH sequence was much faster than that at SDH, as predicted. 32 However, the SDHL tetrapeptide was detected, confirming that both SDH and the SRH cleavage sites were hydrolytically active. The protein product after cleavage at both SGH and SDH sites was also found (Table 1 and Table S1, ESI †). These data revealed that the three Ni(II)-susceptible sequences exerted different activities during the reaction with the Ni(II)  a Separation of the P-1MEY cleavage products by HPLC was performed under acidic conditions thus, the products did not contain complexed metal ions. b (S) and (L) denote the small and large protein products of the hydrolysis at each site. n.d., not detected. For the sequences see Table S1 (ESI). c HPLC peak broadened due to the presence of various oxidation products. The comparison of the calculated and measured masses of the central large (L) protein portions yielded a 6 Da difference, corresponding to oxidation of Cys residues to disulfides. d No oxidation products were detected when the sample was directly taken from the reaction mixture and immediately acidified. e The protein sample was not acidified. The calculated mass reflected the coordination of 3 Â Zn(II) and 1 Â Ni(II) ions. f Oxidation of Cys residues within the destroyed finger unit was detected when the sample was directly taken from the reaction mixture and immediately acidified. g The protein sample was not acidified. The calculated mass reflected the coordination of 2 Â Zn(II) and 1 Â Ni(II) ions. Oxidation of cysteines within the destroyed finger unit was detected.
ion. The hydrolysis at the SDH site was the least favorable one, consistently with previous studies on model peptides. [30][31][32] The mass of the final P-1MEY product of 9720 Da reflects the coordination of 2 Â Zn(II) and 1 Â Ni(II) ions and the oxidation of cysteines within the destroyed 3 rd ZF unit, which lost the Zn(II) ion binding ability after hydrolysis. The ESI MS analysis of the P-1MEY# reaction products at various pH confirmed the identity of the cleaved protein and its metal binding status. The mass obtained under acidic conditions corresponded well to that of the cleaved apoprotein. The mass of the hydrolyzed P-1MEY# protein in a buffered solution was 11 733 Da (Table S1, ESI †). As the calculated mass of the apoprotein is 11485.8 Da, it proves the coordination of three Zn(II) ions within the ZF units and a Ni(II) ion at the N-terminal ATCUN motif formed by the cleavage reaction (the calculated mass of the holoprotein is 11733.6 Da). The thiolate oxidation detected within the 3 rd finger of the original P-1MEY was not observed here due to the fact that the Zn(II) binding sites of the redesigned P-1MEY# protein remained intact during the hydrolytic process.
The functional structure is maintained in the Ni(II)-complexed P-1MEY# cleavage product Circular dichroism spectra of P-1MEY# were recorded before and after the Ni(II)-induced hydrolysis, which resulted in the N-terminal deka-His tag removal. The comparison of these confirmed the preservation of the spectral pattern characteristic for the folded solution structure of a Cys 2 His 2 ZF unit 18,54 in the cleavage product (Fig. 3), even though the reaction was carried out at harsh conditions (50 1C and pH 8.2), and the buffer was exchanged before the CD measurement.
The CD results further confirmed that unwanted modifications such as the cleavage at non-specific sites, removal of Zn(II) ions or oxidative damage of the protein did not occur to a detectable extent in the modified P-1MEY# protein. The slight increase of the CD intensity should be the consequence of the removal of the short disordered N-terminal His-tag (GHHHHHHHHHHS). The CD spectrum of the cleaved P-1MEY protein (Fig. S7, ESI †) is practically identical to that of the intact P-1MEY. This suggests that the ordering effect of the His-tag removal and the disordering effect of the remnant peptide sequence after the cleavage of the 3 rd finger unit partially compensate for each other, and that the structure of the two uncleaved ZF units was maintained during the hydrolytic reaction in good agreement with the MS results. The consequences of editing out the cleavage sites from the DNA recognition region of P-1MEY in the Ni(II)-induced hydrolytic peptide bond cleavage reaction are manifested in Fig. 4.
The question whether the Ni(II)-cleaved P-1MEY# is able to recognize its cognate DNA was investigated by EMSA. The band of the DNA molecule including four copies of the ZF recognition site was already shifted in agarose gel upon addition of one molar equivalent of the protein (Fig. 5). By increasing the protein excess a second distinct shifted band appeared due to the binding of one or more further protein molecules. At a fourfold excess of the protein only the second band was observed. A smear was present to a small extent attributed to minor non-specific binding -natural for all ZF protein as a consequence of the electrostatic component of their interactions with DNA. Similar phenomena were observed with intact P-1MEY#, indicating that both proteins can interact with the favourable DNA sequences equally effectively.

Conclusion
We have tested for the first time the possibility of the specific peptide bond hydrolysis induced by Ni(II) ions within two complex His-tagged ZF metalloproteins without compromising the original metal ion binding sites. The hydrolytic cleavage of the two potential Ni(II)-cleavage sites within the 3 rd finger was not hindered by the presence of Zn(II) ions, but concomitantly it abolished the Zn(II) binding site, resulting in loss of the functional structure of this finger. Such partial cleavage of a ZF type protein may lead to altered DNA binding causing unwanted side-effects in cells. Under the applied conditions the deka-His affinity tag did not interfere with the hydrolytic peptide bond cleavage.
We redesigned the P-1MEY protein to edit out the naturally occurring Ni(II) cleavage sites in the 3 rd ZF unit and to obtain a single N-terminal Ni(II)-catalytic site between the N-terminal His-tag and the ZF protein sequence. The Zinc Finger Tools program was applied to design sequence changes without affecting the DNA binding specificity. We confirmed experimentally that the P-1MEY# protein maintained the Zn(II) and Ni(II)-binding ability and a functional solution structure after the Ni(II)induced protein hydrolysis. This is crucial for any subsequent applications.
Importantly, the Ni(II) ion remains coordinated to the (Ser/Thr)-X-His motif in the C-terminal cleavage product. Such peptide sequence, characterized by the presence of a His residue in position 3 (referred to as ATCUN motif) provides strong Cu(II) or Ni(II) binding and was shown to cleave DNA in the presence of peroxides. 55,56 Functionalization with DNA-binding agents can enhance the efficiency or the selectivity of this reaction, 23,55,[57][58][59][60][61][62][63][64] and may provide a chance for future treatment of Ni(II)-dependent cancer utilizing the abundant Ni(II) ions present there already. [65][66][67][68][69][70][71] The advantage of our approach compared to previous reports is that the ATCUN site of the ZF protein is initially masked by the Ni(II) sensitive sequence, and can be activated and regulated by the addition of Ni(II) ions, temperature jump or pH jump (as the rate of the peptide bond cleavage itself is strictly dependent on these three factors). [30][31][32][37][38][39] Furthermore, the Ni(II) induced peptide-bond hydrolysis can be applied for the removal of any fusion proteins (such as affinity tags, inhibitory or activating domains) from sensitive ZF metalloproteins. Thereby it provides a chance for simple regulation and replacement of the expensive specific protease enzymes. The N-terminal metal-binding site may also promote the application of zinc fingers and eventually their fusion variants as specific carriers of drugs or sensors in their ternary complexes. Recently it has been reported that the ATCUN motif can improve bioactivity of antimicrobial peptides. 72 Zinc finger proteins are potential biotechnological tools for specific gene recognition and manipulation. Although the recent competitive TALE and especially the CRISPR technologies prevail this field, 73-75 ZF protein based factors may still be attractive choice for eukaryotic applications because of their complex protein-DNA interface (the complexity of intra-and inter-finger interactions allows one for discrimination between similar targets), small size, human origin, as well as their low antigenicity. This is reflected in a number of recent publications. 65,[76][77][78] In addition the method described above can be generally applied for any engineered protein, and by optimizing the Ni(II)-induced protein hydrolysis to be significantly accelerated it may significantly contribute to this field.  ESI-MS Electron spray ionisation mass spectrometry. Factor Xa Protease, which recognises IEGR amino acid sequence and cleaves after the arginine. FokI

H2A
Histone protein involved in the structure of chromatin in eukaryotic cells. HEPES 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid commonly used buffer in biochemistry and molecular biology. His-tag A dekahistidine-tag is an amino acid motif in proteins that consists of ten histidine (His) residues. The His-tag makes it possible to purify the protein by Ni (

Conflicts of interest
There are no conflicts to declare.