Issue 41, 2018
From the journal:

Physical Chemistry Chemical Physics

High-frequency pulsed electron–electron double resonance spectroscopy on DNA duplexes using trityl tags and shaped microwave pulses†

A. Kuzhelev,‡abc   D. Akhmetzyanov,‡d   V. Denysenkov,d   G. Shevelev,ae   O. Krumkacheva,ac   E. Bagryanskaya ORCID logo *ab  and  T. Prisner ORCID logo *d  

* Corresponding authors

a Novosibirsk State University, Pirogova 1, 630090 Novosibirsk, Russia

b N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Lavrentiev Avenue 9, 630090 Novosibirsk, Russia
E-mail: egbagryanskaya@nioch.nsc.ru

c International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk, Russia

d Goethe University Frankfurt am Main, Institute of Physical and Theoretical Chemistry, Center for Biomolecular Magnetic Resonance, Max von Laue Str. 7, 60438 Frankfurt am Main, Germany
E-mail: prisner@chemie.uni-frankfurt.de

e Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, 630090 Novosibirsk, Russia

Abstract

Accurate distances between two trityl paramagnetic tags site-specifically attached to DNA duplexes were measured by pulsed electron–electron double resonance spectroscopy at 180 and 260 GHz microwave frequencies. Up to a threefold increase in the sensitivity of 260 GHz PELDOR measurements was achieved by using shaped broad-band microwave pulses.

Graphical abstract: High-frequency pulsed electron–electron double resonance spectroscopy on DNA duplexes using trityl tags and shaped microwave pulses

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Article information

DOI
https://doi.org/10.1039/C8CP03951H
Article type
Paper
Submitted
21 Jun 2018
Accepted
03 Oct 2018
First published
04 Oct 2018

Phys. Chem. Chem. Phys., 2018,20, 26140-26144

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High-frequency pulsed electron–electron double resonance spectroscopy on DNA duplexes using trityl tags and shaped microwave pulses

A. Kuzhelev, D. Akhmetzyanov, V. Denysenkov, G. Shevelev, O. Krumkacheva, E. Bagryanskaya and T. Prisner, Phys. Chem. Chem. Phys., 2018, 20, 26140 DOI: 10.1039/C8CP03951H

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