A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

Majid Rezayi; Majid Ghayour-Mobarhan; Seyedeh Belin Tavakoly Sany; Mona Fani; Amir Avan; Zahra Pasdar; Gordon A. Ferns; Ebrahim Abouzari-Lotf; Iraj S. Amiri

doi:10.1039/C8AY02146E

A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

Majid Rezayi,

*^ab Majid Ghayour-Mobarhan,^ab Seyedeh Belin Tavakoly Sany,

^c Mona Fani,^d Amir Avan,^ab Zahra Pasdar,^e Gordon A. Ferns,^f Ebrahim Abouzari-Lotf

^g and Iraj S. Amiri*^hi

Author affiliations

* Corresponding authors

^a Metabolic Syndrome Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
E-mail: rezaeimj@mums.ac.ir

^b Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

^c Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

^d Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

^e Medical School, University of Aberdeen, Foresterhill, Aberdeen, UK

^f Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN1 9PH, UK

^g Advanced Materials Research Group, Center of Hydrogen Energy, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia

^h Computational Optics Research Group, Advanced Institute of Materials Science, Ton Duc Thang University, Ho Chi Minh City, Vietnam
E-mail: irajsadeghamiri@tdtu.edu.vn

ⁱ Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam

Abstract

Serum 25-hydroxyvitamin D (25(OH)D) is the principal circulating metabolite of vitamin D. Several methods for measuring serum 25(OH)D have been reported. These methods include chromatographic methods (GC-MS, HPLC, and LC-MS), competitive protein-binding assay (CPBA), radioimmunoassay (RIA) and automated methods. In addition, biosensors have more recently been employed as potential candidates for routine analysis of 25(OH)D in serum. In this review, the common methods for quantification of 25(OH)D₂ and 25(OH)D₃ in biological samples, including the preparation of samples, are critically compared and discussed.

This article is part of the themed collection: Recent Review Articles

Article information

https://doi.org/10.1039/C8AY02146E

Article type

Critical Review

Submitted

30 Sep 2018

Accepted

13 Nov 2018

First published

13 Nov 2018

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Anal. Methods, 2018,10, 5599-5612

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A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

M. Rezayi, M. Ghayour-Mobarhan, S. B. Tavakoly Sany, M. Fani, A. Avan, Z. Pasdar, G. A. Ferns, E. Abouzari-Lotf and I. S. Amiri, Anal. Methods, 2018, 10, 5599 DOI: 10.1039/C8AY02146E

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Analytical Methods

A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

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A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

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