Neutrophil membrane camouflaged hybrid nanozymes for enhanced starvation/photothermal tumor therapy

Abstract

Glucose oxidase (GOx) triggered starvation therapy has attracted great attention in cancer treatment, which is tremendously inhibited under a tumor hypoxia environment. Here a neutrophil membrane camouflaged hybrid nanozyme (GOx/HPB/NEM) is fabricated based on hollow Prussian blue loaded with GOx for enhanced starvation/photothermal tumor therapy. The hybrid nanozyme possesses catalase properties that originated from Prussian blue to produce oxygen from H₂O₂, which consequently reacts with glucose catalyzed by GOx. Thus GOx-driven oxidation effectively eliminates glucose inside tumors for starvation therapy. Meanwhile, adenosine triphosphate (ATP) production in tumor cells is inhibited, accompanied by heat shock protein (HSP) depletion. Furthermore, hollow Prussian Blue (HPB) mediated photothermal therapy (PTT) is coupled with GOx-mediated starvation therapy to obtain more effective tumor therapy. The neutrophil membrane on the nanozyme surface helps noninvasive tumor active targeted accumulation. In vivo studies demonstrate that the GOx/HPB/NEM nanozyme presents remarkable capability in tumor ablation with a tumor growth inhibition rate of 97.7%. The proposed treatment paradigm may encourage more hybrid nanozyme nanoplatforms involving effective cancer therapy.