Issue 1, 2017

Fluorometabolite biosynthesis: isotopically labelled glycerol incorporations into the antibiotic nucleocidin in Streptomyces calvus

Abstract

Deuterium and carbon-13 labelled glycerols have been fed to Streptomyces calvus fermentations and isotope incorporation into the fluorine containing antibiotic nucleocidin have been evaluated by 19F-NMR. A single deuterium atom was incorporated from [2H5]- and (R)-[2H2]-glycerol into C-5′ of the antibiotic, suggesting that an oxidation occurs at this carbon after ribose ring assembly from glycerol (pentose phosphate pathway), during nucleocidin biosynthesis.

Graphical abstract: Fluorometabolite biosynthesis: isotopically labelled glycerol incorporations into the antibiotic nucleocidin in Streptomyces calvus

Supplementary files

Article information

Article type
Communication
Submitted
19 Oct 2016
Accepted
07 Nov 2016
First published
09 Nov 2016

Org. Biomol. Chem., 2017,15, 61-64

Fluorometabolite biosynthesis: isotopically labelled glycerol incorporations into the antibiotic nucleocidin in Streptomyces calvus

A. Bartholomé, J. E. Janso, U. Reilly and D. O'Hagan, Org. Biomol. Chem., 2017, 15, 61 DOI: 10.1039/C6OB02291J

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