Singlet oxygen formation on bio-inspired synthesis of hollow Ag@AgBr photocatalyst for microbial and chemical decontamination

All chemicals were of at least analytical grade and used as received without further purification. Tryptic Soy Broth (TSB), Yeast Extract (YE) and BBLTM TrypticaseTM Peptone in powder form were purchased from BD Company. The 1% w/v YE and 2% w/v peptone solutions were prepared by suspending the powder in 1 L deionized (DI) water followed by autoclave at 121 oC for 15 min. Silver nitrate, sodium chloride, sodium bromide and 2,2,6,6tetramethyl-4-piperidinol (TMP, 99%) were purchased from Sigma–Aldrich. Silver nitrate was dissolved in DI water to get an 80 mM stock solution. In a typical procedure of Ag@AgBr hollow nanoparticle preparation, 5 mL AgNO3 solution was added to 2.5 mL mixture of YE and peptone solution followed by addition of 2.5 mL (400 mM) NaBr. The resulting milky white solution was then magnetically stirred under a solar simulator (550 W Xenon lamp, ABET Technologies, Model 11016A Sun 3000) for 5 min. At the same time, a series of silver chlorobromide (AgClxBr1-x, x = 1, 0.64, 0.36) nanoparticles were synthesized by adjusting the molar ratio of Clto Br-. All nanoparticles were centrifuged down at 7000 rpm for 30 min and the pellet was washed by water twice. After the washing steps particles were resuspended in 5 mL DI water for further use. For comparison, Ag@AgBr particles prepared in DI water without yeast extract and peptone involvement was named as Ag@AgBr-DI.

Worldwide, the improper disposal of wastewater and sludge has resulted in the pollution of numerous water resources with both hazardous chemicals and pathogenic microbes.A particular concern in many developing countries has been microbial contamination.To get safe drinking water, chlorination is the most widely used wastewater treatment method.However, the toxic disinfection by-products (DBPs) formed in chlorinated wastewaters may have an adverse impact on aquatic organisms. 1Photocatalysis is an alternative process that can be used in water purification. 2,3A great amount of research in the solar-light-driven catalysis field has been aimed at developing semiconductor nanomaterials, such as TiO 2 , which not only decompose toxic organic compounds but also kill bacteria. 4,5Reactive oxygen species (ROS) formed during a photocatalytic process are reported to be nonselective oxidizing agents for many organic pollutants.Similarly, ROS can kill pathogens by causing oxidative damage to essential bio-macromolecules. 6][9][10][11] Coactively, the Ag + released from Ag@AgX could bind to DNA and other sulphur-, nitrogen-and phosphorus-containing components, followed by damaging bacterial cells with multiple modes from interfering with DNA replication to inactivating respiratory enzymes. 12,13Therefore, the exploration of multifunctional Ag@AgX nanohybrids for environmental applications has witnessed exciting advancements over the past decade.
We have successfully synthesized Ag@AgCl in aqueous LB broth (Miller's) solution at room temperature with 5 min sunlight exposure.5][16] The broth alone strategy dramatically simplifies the conventional microbial process by cutting the use of microorganisms and thus provides a greener way for nanomaterial preparation.Moreover, the biogenic Ag@AgCl outperforms commercial TiO 2 in decomposing organic dyes under solar light with excellent photocatalytic stability.Based on these works, we attempted to fabricate high quality hollow Ag@AgBr nanostructures for water remediation, since hollow structural materials usually show improved catalytic properties due to their high surface area, low density, and porous structure.The enhancement is achieved by allowing greater light absorption/reflection into the interior and increasing mass transfer as well as providing more reaction sites. 17,18ifferent from the template-assisted synthesis of hollow/ porous AgBr/Ag composites, [19][20][21] our nano Ag-incorporated AgCl x Br 1−x has been obtained by the precipitation of AgNO 3 with sodium chloride in peptone solution first, followed by the nanoscaled Kirkendall process (the ion-exchange reaction between Br − and AgCl due to the large difference between the solubility of AgBr and AgCl) and 5 min solar light irradiation.Peptone is produced by the partial hydrolysis of animal tissues, milk, plants or microbial cultures.NaCl is usually formed during the neutralization of acid hydrolysis, resulting in a product with a medium to high salt content.The photocatalytic activities of the Ag@AgBr nanocrystals were evaluated for methyl orange (MO) degradation and E. coli disinfection.Electron spin resonance (ESR) spectroscopy was employed to study the ROS involved in the reaction.Except for the well-recognized ˙OH and O 2 ˙−, 22 singlet oxygen ( 1 O 2 ) was directly identified for the first time as a contributor to the degradation of pollutants using Ag@AgBr catalysts.
Structure and morphology characterization of Ag@AgCl x Br 1−x nanocrystals Fig. 1a shows the XRD patterns of nanocrystals synthesized with different concentrations of Cl − .The Ag@AgCl sample exhibits diffraction peaks at 27.9°, 32.3°, 46.3°, 54.9°, 57.5°, and 67.4°, corresponding to the (111), ( 200), ( 220), ( 311), (222), and (400) planes of face-centered cubic (fcc) phase AgCl (JCPDS file: 31-1238). 23The diffraction peaks of Ag@AgBr at 26.7°, 31.0°,44.4°, 52.6°, 55.0°, 64.5°and 73.3°c an be indexed to the (111), ( 200), ( 220), ( 311), ( 222), (400), and (420) planes of cubic phase AgBr (JCPDS file: 06-438). 24here are no obvious peaks of metallic Ag due to the small size of the photo-reduced Ag NPs.Interestingly, Ag@AgCl 0.64 Br 0.36 and Ag@AgCl 0.36 Br 0 .64 particles display a pure phase of fcc lattice close to Ag@AgBr.Changing the composition from chloride-rich to bromide-rich causes a monotonic peak shift towards smaller angles.Our results indicate that, in the presence of yeast extract and peptone, the co-precipitation of Ag + between Cl − /Br − leads to the formation of a homogeneous solid solution phase of AgCl and AgBr.This result is consistent with previous experimental works. 25he optical properties of the synthesized nanostructures were determined using UV-vis absorption spectroscopy.As shown in Fig. 1b, owing to the surface plasmonic resonance effect of the nano Ag, all Ag@AgCl x Br 1−x samples show stronger absorption in the visible region than AgCl; with increasing [Br − ], the absorption peak at 266 nm corresponding to the direct bandgap of AgCl was gradually replaced by a stronger peak at 321 nm associated with the direct bandgap of AgBr.
The particle z-average sizes and polydispersity index (PDI) were obtained by DLS measurements (Table S1 †).Two nano silver-incorporated AgCl and AgBr samples had z-average diameters around 120 nm.At 25 °C, the solubility product constants (K sp values) of AgBr and AgCl are 5.0 × 10 −13 and 1.8 × 10 −10 , respectively.The nucleation of AgBr is faster; therefore one would expect two different populations of AgBr and AgCl in the Ag@AgCl 0.36 Br 0.64 and Ag@AgCl 0.64 Br 0.36 samples, but the PDI of these mixed silver halide particles was very low (<0.1).This supports the homogeneity of the XRD results in Fig. 1a.
Representative FESEM images of Ag@AgCl and Ag@AgBr (Fig. 2a-d) reveal that these spherical samples were approximately 110 nm in diameter.Solid Ag@AgCl with a relatively wide size distribution is roughly covered with tiny silver nanoparticles (indicated by white arrows).The hollow nature of Ag@AgBr nanostructures is clearly shown in Fig. 2d.The average pore diameter determined based on the desorption of nitrogen was approximately 8.7 nm.The BET measurement revealed that the as-synthesized Ag@AgBr had a surface area of 5.98 m 2 g −1 , which is comparable to previously reported data. 26It is difficult to obtain a high quality TEM image of Ag@AgBr due to the problem with fast reduction of AgBr NPs by the electron beam of TEM.Fig. 2e shows a HAADF-STEM image of Ag@AgBr NPs and the corresponding EDX maps.In contrast to most hollow Ag decorated halides obtained by NaBH 4 reduction which are Ag-rich on the surface, 18 here, the EDX elemental maps show pronounced Br signals at the outermost parts of the particles.
Based on the above-mentioned results, we propose a possible reaction route and the structural evolution of the hollow spheres, as shown in Scheme 1.The first step is the instantaneous formation of AgCl nuclei after mixing AgNO 3 with a YE and peptone solution.With the help of biomolecules provided by YE and peptone, the nuclei aggregate into welldispersed solid AgCl nanospheres with a diameter around 120 nm (Table S1 †).The introduction of NaBr leads to the surface conversion of AgCl to AgBr driven by the decrease in enthalpy of precipitation of the silver halides. 27Because Ag + cations diffused outward more quickly than Br − diffused inward, nanoscaled Kirkendall voids were generated near the AgCl/AgBr interface. 19,28Upon solar light irradiation, AgBr is excited to generate electrons at the conduction band and then react with Ag + to form Ag NPs across the surface.Meanwhile, biomolecules like peptide or DNA adsorbed on AgBr might facilitate the cleavage of AgBr NPs due to high energy UV exposure.The Ag NPs are able to absorb visible light and drive a collective and coherent oscillation of the electrons with the incident photons due to the localized surface plasmon resonance (LSPR).The decay of LSPR or the electron-phonon relaxation is generally coupled with the vibrations of the Ag and AgBr lattices and heating of the surrounding local environment.An extremely high transient temperature of the irradiation location promoted the Ostwald ripening process by enhancing the dissolution rate of smaller AgBr nanoparticles. 14

Photocatalytic activities of Ag@AgCl x Br 1−x nanoparticles
The photocatalytic efficiency of the samples was evaluated by studying the degradation of methyl orange (MO) dye.Fig. 3 shows the variation of MO characteristic peak intensity as a function of irradiation time.After the adsorption-desorption process in the dark for 30 min, the change in the concentration of MO molecules was monitored by measuring the absorbance at 463 nm.The adsorption fraction of MO on the photocatalyst was given by (A1 − A2)/A1, where A1 is the absorption of 10 ppm MO solution and A2 is the absorption of the dye solution after reaching equilibrium.The accumulation of MO on the hollow Ag@AgBr particles was 7%.It is known that an appropriate adsorption capacity of photocatalysts is beneficial to the catalytic activity. 29Under solar light, Ag@AgCl decomposed 47% of MO after 8 min irradiation.The photocatalytic degradation rate was enhanced by tuning the composition from Cl − rich to Br − rich, as 71% and 76% MO were decomposed by Ag@AgCl 0.64 Br 0.36 and Ag@AgCl 0.36 Br 0.64 , respectively.The Ag@AgBr sample exhibits the highest photocatalytic degradation efficiency, and 81.0% of MO could be degraded within 8 min.For comparison, Ag@AgBr particles prepared in deionized (DI) water (named Ag@AgBr-DI) without yeast extract and peptone involvement was evaluated for the degradation of various dyes under visible light irradiation.Ag@AgBr-DI exhibited a much lower photocatalytic performance, as shown in Fig. S1.† To evaluate the durability of the catalysts, three successive methyl orange degradation tests under solar light were  carried out and the removal of the dye was still 90% after two times of recycling.Additionally, as shown in Fig. S2, † even after exposure to sunlight for 1 hour, the XRD patterns of Ag@AgBr particles showed no apparent change.The photocatalytic stability of Ag@AgBr may be ascribed to the electron confining property of plasmonic Ag NPs that prevents photolysis of AgBr to Ag nanoclusters. 14

Antimicrobial test
Silver-based antimicrobial agents are gaining tremendous attention in various fields due to their broad spectrum activity, efficacy and low cost.Many studies have suggested that both dissolved and nanoparticulate silver contribute to the biocidal property of Ag materials, with Ag + as the main germicidal source. 30,31However, free Ag + has ample opportunities to react with Cl − , SO 4 2− , and CO 3 2− in the culture media and the wastewater, which therefore gave rise to changes in its potency. 32Moreover, under different lighting conditions, bacterial cells exhibited variable susceptibility to Ag + . 33As shown in Fig. 4A and C, the biocidal activities of the Ag chlorobromides were evaluated by testing the growth inhibition concentrations against E. coli in the dark first.The MIC for the silver ions was determined to be 3.12 ppm against E. coli, which matched well with previous studies. 13The growth of E. coli was inhibited by Ag@AgCl and Ag@AgBr at 6.25 and 12.5 ppm, respectively.The formation of solid Ag@AgX and Ag NPs on the surface of Ag@AgCl x Br 1−x hindered the elution of Ag + from the particles, resulting in lower [Ag + ] and E. coli being less sensitive to Ag@Ag chlorobromides in the solution. 34After exposing the plates to solar light for 30 min, a reverse trend was observed with the MIC values: Ag@AgBr (6.25 ppm) < Ag@AgCl (12.5 ppm) < AgNO 3 (25 ppm).The enhanced antibacterial ability of Ag@AgBr could be attributed to the synergistic action of free radicals, such as ˙OH and O 2 ˙−, and photogenerated holes (h + ) on the surface of photocatalysts. 8These oxidizing agents do not have specificity towards microbial cells when compared with mammalian cells. 35In contrast, another kind of ROS, singlet oxygen ( 1 O 2 ), is more toxic to prokaryotic cells than to eukaryotic cells. 36Moreover, 1 O 2 is one of the most acutely lethal ROS to bacterial cells because no enzyme can detoxify it.8][39] In this study, ESR spectroscopy combined with 2,2,6,6-tetramethyl-4piperidinol (TMP) was employed to probe the generation of 1 O 2 , since the specific 1 O 2 oxidation of TMP to the 2,2,6,6tetramethyl-4-piperidinol-N-oxyl radical (TMPN) was detectable by ESR. 40s shown in Fig. 5a, the time-dependent increase in ESR signal was obvious following the irradiation of TMP in the presence of Ag@AgBr.The characteristic 1:1:1 triplet could be unequivocally attributed to TMPN.The signal intensity of TMPN was decreased after adding methyl orange, indicating the competitive consumption of 1 O 2 by MO molecules.
Garg et al. have determined the main reactive species produced on irradiation of AgCl(s) and proposed the 1 O 2 generation as a result of H 2 O 2 reaction with free chlorine (OCl − ). 41It has been known that the yield of singlet oxygen is 76% with H 2 O 2 and hypobromite. 42Based on previous works, we propose the following 1 O 2 generation process.Eqn (1) describes the absorption of a photon by a Ag@AgBr crystal and the creation of a positive hole in the valence band and an electron in the conduction band.The electrons tend to move to the silver nanoparticles on the surface and may react with O 2 to give a superoxide anion and the more stable H 2 O 2 (eqn ( 2) and ( 3)).On the other hand, bromide ions (Br − ) may capture the photogenerated hole on the irradiated AgBr to form a bromine atom.This species then reacts rapidly with bromide to produce Br 2 ˙−.Two of such anion radicals disproportionate to give bromine (Br 2 ) and subsequently hydrolyze to form

Fig. 2
Fig. 2 Morphological images of Ag@AgCl (a and b) and Ag@AgBr (c and d).(e) HAADF-STEM image and the corresponding HAADF-STEM-EDS maps of different elements, showing the distribution of Ag and Br elements in Ag@AgBr.

Fig. 3
Fig.3Methyl orange degradation rate using nano Ag-incorporated silver halide samples.

Fig. 4
Fig. 4 Minimum inhibitory concentration (MIC) evaluation of Ag@AgCl (A and B) and Ag@AgBr (C and D) samples.

Fig. 5
Fig. 5 Electron spin resonance spectra of aqueous suspensions of Ag@AgBr with TMP only (a) and with TMP plus methyl orange (b) after 12 min light irradiation.