A bodipy based hydroxylamine sensor

Hydroxylamine, (HA) NH2OH, is an oxygenated form of ammonia, which is widely used in industrial and pharmaceutical processes. HA is widely known as a nitric oxide (NO) donor and participates in a wide range of biological processes. In cellular metabolism, HA is an intermediate in the conversion L-arginine to nitric oxide (NO). This process involves the hydrolysis of oxime arginine to L-citrulline and HA. HA is then converted by catalase to NO and superoxide ( O2) in the presence of hydrogen peroxide. 7 It is also believed that HA can be converted to NO by the non-enzymatic attack by O2. Therefore, in inflammatory cells a reaction between HA and O2 could take place to generate NO. 3,8 For example, HA has shown to be a vasodilator in a dose-dependent manner in the blood vessels of rats’ kidneys. HA has also shown to be an inhibitor for the release of insulin and HA has been shown to activate K channels. Despite HA being a product of metabolism and a NO donor, it is moderately toxic to humans, animals and plants. HA toxicity occurs at concentrations that are substantially greater than the normal physiological concentrations. Exposure to HA has been shown to be hemotoxic, mutagenic whilst also being an enzyme and virus inhibitor. Given these important biological aspects of HA we set out to develop a fluorescence probe for the detection of HA. To our knowledge, there are currently no HA fluorescence probes. HA is currently detected using: HPLC, GC, potentiometric, polarographic, biamperometric and electrochemical methods. Previously, we have developed a synthetic route to nitrones by reacting an aromatic aldehyde containing an ortho a,b-unsaturated ester with NH2OH (50% wt in H2O) in THF at 20 1C to produce isoindole nitrones in high yields, Scheme 1. Therefore, we set out to integrate this reaction with a fluorophore for the detection of HA. Luckily, an appropriate system (probe 1) had previously been prepared for the detection of hydrogen sulphide (H2S) 21,22

Hydroxylamine, (HA) NH 2 OH, is an oxygenated form of ammonia, which is widely used in industrial and pharmaceutical processes. 1 HA is widely known as a nitric oxide (NO) donor and participates in a wide range of biological processes. [2][3][4] In cellular metabolism, HA is an intermediate in the conversion L-arginine to nitric oxide (NO). 5,6 This process involves the hydrolysis of oxime arginine to L-citrulline and HA. HA is then converted by catalase to NO and superoxide ( À O 2 ) in the presence of hydrogen peroxide. 7 It is also believed that HA can be converted to NO by the non-enzymatic attack by À O 2 . Therefore, in inflammatory cells a reaction between HA and À O 2 could take place to generate NO. 3,8 For example, HA has shown to be a vasodilator in a dose-dependent manner in the blood vessels of rats' kidneys. 9 HA has also shown to be an inhibitor for the release of insulin and HA has been shown to activate K + channels. 3,10 Despite HA being a product of metabolism and a NO donor, it is moderately toxic to humans, animals and plants. HA toxicity occurs at concentrations that are substantially greater than the normal physiological concentrations. Exposure to HA has been shown to be hemotoxic, mutagenic whilst also being an enzyme and virus inhibitor. 11 Given these important biological aspects of HA we set out to develop a fluorescence probe for the detection of HA. To our knowledge, there are currently no HA fluorescence probes. HA is currently detected using: HPLC, GC, potentiometric, polarographic, biamperometric and electrochemical methods. [12][13][14][15][16][17][18][19] Previously, we have developed a synthetic route to nitrones by reacting an aromatic aldehyde containing an ortho a,b-unsaturated ester with NH 2 OH (50% wt in H 2 O) in THF at À20 1C to produce isoindole nitrones in high yields, 20 Scheme 1. Therefore, we set out to integrate this reaction with a fluorophore for the detection of HA. Luckily, an appropriate system (probe 1) had previously been prepared for the detection of hydrogen sulphide (H 2 S) 21,22 Scheme 1.
The synthesis of probe 1, is given in the ESI. † We also confirmed that probe 1 reacts as predicted with hydroxylamines as shown by the 1 H NMR, 13 C NMR and MS (ESI †). More importantly, when HA is added to a 0.5 mM solution of probe 1 in a 1% DMSO PBS Buffer solution at pH 7.4 the fluorescence is significantly enhanced as the concentration of HA is increased (Fig. 1).
From these results, it is clear that the system works for simple primary hydroxylamines (1) hydroxylamine, (2) N-(methyl)hydroxylamine (3) N-(benzyl)hydroxylamine, (4) N-(propargyl)hydroxylamine, however, larger alkyl groups or aromatic hydroxylamines such as (5) N-(tert-butyl)hydroxylamine and (7) N-(phenyl)hydroxylamine do not work. It is evident that the system also requires the hydroxyl of the hydroxylamine (6) O-(benzyl)hydroxylamine. These observations are consistent with the proposed mechanism of the reaction (Scheme 2). Where, a nucleophillic nitrogen 23 and exchangeable OH are required to facilitate condensation of hydroxylamines with probe 1.
We have carried out a more extensive screen amongst relevant biological species (ESI †), and as expected only H 2 S (50 mM) produced a fluorescence response.
We then attempted to detect hydroxylamines in a cellular environment using probe 1. HeLa cells were incubated HA (0-150 mM) for 30 minutes and washed with DPBS then 1 mM of probe 1 was added and left for 30 minutes, fluorescence images were then acquired by confocal microscopy. From these fluorescence images it is clear that probe 1 was fluorescent in HeLa cells even without added HA (ESI, † Fig. S3). The strong background fluorescence is attributed to cellular thiols including endogenous H 2 S present in the HeLa cells.
In conclusion, we have developed a fluorescence turn-on probe for the detection of hydroxylamines. The system works well in vitro but with the in vivo cellular experiments endogenous thiols react to turn on probe 1. We are currently working to develop a system with enhanced selectivity for hydroxylamines over thiols. However, it is interesting to note that the system using N-(propargyl)hydroxylamine could be used as fluorescent ''click'' tag.
We would like to thank the EPSRC and the University of Bath for funding. ACS, JEG, and LRP thank the EPSRC for studentships. RSLC thanks the EPSRC Doctoral Training Centre in Sustainable Chemical Technologies: EP/G03768X/1 for a studentship. JY thanks to the support from the National Research Foundation of Korea (NRF), which was funded by the Korea government (MSIP) (No. 2012R1A3A2048814). Catherine Lyall is thanked for help in obtaining NMR spectra using NMR characterisation facilities provided through the Chemical Characterisation and Analysis Facility (CCAF) at the University of Bath (www.bath.ac.uk/ccaf). The EPSRC UK National Mass Spectrometry Facility at Swansea University is thanked for analyses.

Conflicts of interest
There are no conflicts to declare.