. Diamond-coated ‘black silicon’ as a promising material for high-surface-area electrochemical electrodes and antibacterial surfaces. Journal of Materials Chemistry B, 34(34),

This report describes a method to fabricate high-surface-area boron-doped diamond (BDD) electrodes using so-called ‘black silicon’ (bSi) as a substrate. This is a synthetic nanostructured material that contains high-aspect-ratio nano-protrusions, such as spikes or needles, on the Si surface produced via plasma etching. We now show that coating a bSi surface composed of 15 m m-high needles conformably with BDD produces a robust electrochemical electrode with high sensitivity and high electroactive area. A clinically relevant demonstration of the eﬃcacy of these electrodes is shown by measuring their sensitivity for detection of dopamine (DA) in the presence of an excess of uric acid (UA). Finally, the nanostructured surface of bSi has recently been found to generate a mechanical bactericidal eﬀect, killing both Gram-negative and Gram-positive bacteria at high rates. We will show that BDD-coated bSi also acts as an eﬀective antibacterial surface, with the added advantage that being diamond-coated it is far more robust and less likely to become damaged than Si.


A. Introduction
Diamond films grown by chemical vapour deposition (CVD) are finding an increasing number of applications due to the superlative properties of diamond coupled with their rising availability and affordability from a number of commercial suppliers. 14][5][6][7][8] These properties make BDD electrodes an exciting alternative to more traditional carbon allotrope electrodes such as glassy carbon, pyrolytic graphite and highly ordered pyrolytic graphite, making many electrochemical applications more attractive and viable. 9,10The pharmaceutical industry has begun utilising BDD electrodes for electroanalysis, which can be used to successfully detect a diverse range of biological molecules including caffeine, 11 glucose, 12 organic acids, serotonin and histamine, 14 purines, 15 and even cofactors such as nicotinamide adenine dinucleotide. 16,17BDD electrodes can also be used for water treatment and purification, 18 for detecting trace amounts of contaminants in water via anodic stripping voltammetry, 19 electrosynthesis of organic 20 and inorganic compounds, neural interfacing, 22,23 and the fabrication of supercapacitors. 24][27][28][29] In particular, structuring the diamond surface on the micro-or nano-scale has the effect of greatly increasing the effective electrode surface area, leading to higher sensitivity, increased selectivity, and higher capacitance values. 30,31Previous attempts to fabricate nanostructured BDD electrodes involved two approaches: either etching away areas of an existing BDD film or depositing the BDD onto an already structured scaffold. 32For the first approach, diamond can be etched using an O 2 -based reactive ion etch (RIE) process and a non-erodible mask made from metals (such as Al, Au, Mo or Ni), oxides (SiO 2 , Al 2 O 3 ) or nanodiamond particles. 33Such techniques have allowed diamond electrodes to be etched into the form of nanopillars, 34 nanowires, 35 nanoneedles 36,37 and nanowhiskers. 38,39In a similar way, extremely porous nanohoneycomb diamond structures have been produced by etching diamond films using an oxygen plasma through a porous alumina mask. 40he second approach involves diamond coating existing nanostructured substrates such as silicon 25 or carbon nanotubes (CNTs) using CVD. 41Recently we reported that 'forests' of vertically aligned CNTs can be coated with nanocrystalline BDD, and in doing so, the CNTs clump together into bundles resembling 'teepees', 42 as well as ridge, web and honeycomb structures. 30Such diamond-coated CNT nanostructures exhibited an increase in surface area and capacitance of up to 450 times when compared to flat BDD electrodes. 30,43The enhanced effectiveness and increased capacitance of diamond-coated CNT nanostructures make them suitable for multi-electrode array sensors for applications in neural prosthesis, neural stimulation, and brain-machine interfaces. 22ather than coat CNTs, another approach is to pre-pattern the substrate material, usually Si, and then coat these micro/ nanostructures with BDD.Peng and co-workers 44 used an electroless metal deposition process to deposit micromasks onto a Si surface, which was then etched away leaving vertically aligned Si nanowires.These were then coated in BDD by CVD to produce a high-area electrode. 25The tips of the diamond 'nanoforests' were then electrochemically functionalized and consequently have been targeted for applications in biochemistry, in particular, as a non-enzymatic glucose sensor with high selectivity and stability. 32his report describes an alternative method to fabricate high-surface-area BDD electrodes using a different type of prepatterned Si.So-called 'black silicon' (bSi) is a synthetic nanostructured material that contains high-aspect-ratio nanoprotrusions, such as spikes or needles, on its surface produced through a simple plasma etching technique.An extensive review of plasma etching can be found in reference. 457][48] Originally something to be avoided, bSi has now found applications photovoltaics [49][50][51] and later used for biomedical sensing applications. 52,53We now show that coating a bSi surface conformably with BDD produces a robust, sensitive electrode with high electroactive surface area.The electrochemical responses are initially assessed using a simple outersphere electron transfer probe (ferri/ferrocyanide).
However, a more clinically relevant test for the efficacy of these electrodes is to measure their sensitivity for detection of dopamine (DA) in the presence of an excess of uric acid (UA). 54A is an important biomolecule which exists at very low concentrations in the body, but which plays an important role in the function of the central nervous, endocrine, cognition and emotion systems.Electrochemical methods have been used previously for determination of DA concentrations, however the coexistence of ascorbic acid (AA) and uric acid (UA) with similar oxidation potentials as DA, and at concentrations much higher than those of DA, cause overlapping voltammetric responses.Thus, a sensitive and accurate electrode for detection of DA in the presence of UA and/or AA is needed.Flat, traditional electrodes are nowhere near sensitive enough for simultaneous detection of DA, UA and AA.Therefore, modified materials such as metal nanoparticles and oxides, CNTs, and polymers, have been used to improve the selectivity, sensitivity, and stability of the electrode. 54We will demonstrate that BDD-coated bSi electrodes can also easily detect DA in the presence of UA.
Finally, the nanostructured surface of bSi has recently been found to generate a mechanical bactericidal effect, killing both Gram-negative and Gram-positive bacteria at high rates. 55We will show that BDD-coated bSi also acts as an effective antibacterial surface, with the added advantage that being diamond-coated it is far more robust and less likely to become damaged than Si.

Black Si fabrication
Two types of bSi substrates were prepared by plasma etching of n-doped single-crystal silicon (100) wafers.Two different inductively coupled plasma (ICP) etching processes 45 were used resulting in a bSi wafer of short needles (B1 mm in length) and a wafer of long needles (B15-20 mm in length), as shown in Fig. 1.The short needles were produced in an Oxford Instruments System 133 fitted with an ICP380 source.The conditions were Cl 2 (48 sccm), O 2 (2 sccm), 15 mTorr pressure, 600 W ICP power, 100 W reactive ion etch (RIE) bias power, 20 1C electrode, 10 min etching time.The long needles were formed in an Oxford Instruments System 100 fitted with a Cobra300 ICP source.A cryogenic etch 47,48,[56][57][58] was used as follows: SF 6 (60 sccm), O 2 (10 sccm), 10 mTorr pressure, 800 W ICP power, 6 W RIE power, À110 1C electrode, with 10 Torr He applied to the backside of the wafer for effective cooling, 30 min etching time.These long needles are some of the longest bSi features reported to date.Both these wafers were cleaved into multiple identical square samples B1 cm 2 in size suitable for electrochemical testing.

Diamond deposition
The bSi samples were then seeded using a suspension of detonation nanodiamond (DND) in methanol using an electrospray process 59 in which the DND suspension was placed under high potential difference (35 kV) with respect to the grounded sample.Due to electrostatic attraction the suspension sprayed onto the sample and coated all the surfaces, including the vertical sides of the needles, with a near monolayer of B10 nm DND seeds.The seeded samples were then placed into a hot filament CVD (HFCVD) reactor, and B0.25 mm of either (i) heavily borondoped microcrystalline diamond (MCD), or (ii) heavily borondoped nanocrystalline diamond (NCD), was deposited using standard CVD conditions: 20 Torr pressure, Ta filament at 2400 K, substrate temperature B900 1C, (i) 1%CH 4 /H 2 gas mixture + 2000 ppm B 2 H 6 , (ii) 4%CH 4 /H 2 gas mixture + 2000 ppm B 2 H 6 . 1,2he level of B doping was sufficient to ensure that in both types of film the diamond had near-metallic conductivity.The resulting diamond-coated bSi samples are shown in Fig. 2. Due to the heavy B doping and the submicron thickness of the diamond layer, the diamond grain size is small (o100 nm) so the MCD film appears rounded with only small facets visible.The NCD film appears even more rounded with no signs of facets.The short bSi needles were coated uniformly with diamond, leaving a surface which was slightly rounded compared to the uncoated needles (Fig. 2(a)).The long needles, however, became diamond coated conformally and uniformly all down their length, with complete coverage occurring after 1 h growth (Fig. 2(b and c) for MCD, and Fig. 2(e and f) for NCD).A further sample was fabricated by depositing NCD onto the long needles for 2 h, leading to the rounded diamond nanocrystallites beginning to fill in the spaces between the needles until they were almost, but not completely, encapsulated.This led to a highly porous but flatter film (Fig. 2(f and g)).Growth times 42 h led to non-porous diamond films with the bSi needles completely embedded within a solid diamond layer.
Other electrodes were prepared for comparison in the electrochemical tests.A standard commercial microcrystalline BDD sample (Element Six, electrochemical grade) was used as the control.This was essentially a flat electrode, although the surface had crystalline facets of size B20 mm (Fig. 2(f)).

Electrode fabrication
For use as the working electrode, the BDD-coated bSi and BDD flat samples were immobilized on a copper plate (1.0 Â 1.0 cm 2 ) using conductive silver paste and sealed using 2 different methods, depending upon which laboratory performed the experiments.For measurements conducted in Brazil, the electrodes were sealed with epoxy resin, leaving an area of about 8 Â 8 mm of the BDD surface exposed.For measurements made in Bristol, Teflon tape with a 7 mm 2 hole (which defined the geometrical area of the electrode) was placed on top of the samples leaving part of the electrode surface exposed at the corner of the sample with which to form an electrical connection.Conductive silver paint was applied to this region and copper tape applied on top (which was then connected to the potentiostat) before finally being covered in Teflon tape.This top connection was more reliable than a back connection, as it eliminated conduction through the Si substrate -however it still relied on there being full connectivity across the surface of the film from the exposed electrode area to the edge contact.The electrochemical results from both laboratories using the different sealing methods were almost identical, with the only difference being in the area of electrode exposed which determined the absolute magnitude of the measured current.

Electrochemical analysis
The various electrodes were analysed electrochemically via cyclic voltammetry (CV) using two aqueous solutions: (i) a potassium nitrate solution (0.1 M) and (ii) a potassium ferricyanide (1 mM) with potassium chloride (0.1 M) supporting electrolyte solution, utilising a mAutolab Type III potentiostat.The electrolyte solution (20 ml) was placed into a glass three-electrode electrochemical cell along with the diamond working electrode, a Ag/AgCl (3.0 M KCl) reference electrode, and a Pt counter electrode.A conditioning step was applied before every scan which entailed applying the starting voltage for 120 s followed by a 10 s equilibration.

Biomolecule analysis and detection
As the BDD-coated (Fig. 2(b and c)) and partially overgrown bSi long needle (Fig. 2(f and g)) samples had proven (see later) to be the most sensitive electrodes in the above KNO 3 and ferri/ ferrocyanide systems, these two electrodes were chosen for trials of DA and UA detection, with the usual flat (Element Six) BDD electrode as control.First their electrochemical response towards DA was determined using an aqueous 0.2 M phosphate buffer solution (pH = 7.0) containing 0.2 mM of DA.CVs were performed between À0.2 and +0.7 V at a variety of potential scan rates between 10 mV s À1 and 400 mV s À1 .Then, linear sweep voltammograms were recorded in the same buffer solution but with varying concentrations of DA from 5.0 Â 10 À6 to 1.0 Â 10 À4 M to determine the analytical sensitivity of the different electrodes towards DA voltammetric sensing.These two experiments were then repeated with UA using the same buffer solution.
Differential pulse voltammograms were then recorded in the same buffer solution with both DA and UA present, with (i) the UA concentration constant at 3.0 Â 10 À5 M while varying the DA concentration from 3.0 Â 10 À7 to 5.0 Â 10 À6 M, and (ii) the DA concentration constant at 3.0 Â 10 À6 M while varying the UA concentration from at 3.0 Â 10 À6 to 5.0 Â 10 À5 M. This was to ensure the electrode could detect the presence of one analyte in the presence of excess of the other.Finally, differential pulse voltammograms were recorded in the same buffer solution with both DA and UA present in varying concentrations.From these, the analytical parameters for the simultaneous voltammetric determination of DA and UA were recorded, including the linear concentration range, analytical sensitivity and limit of detection (LOD).The LODs were calculated using the relation 3s/m, where s is the standard deviation of ten blank (supporting electrolyte only) measurements and m is the slope of the analytical curve (analytical sensitivity).In addition, the stability of response of the proposed electrode material toward DA and UA detection was evaluated from repeatability studies.

Bactericidal surface testing
In this preliminary trial, only the short BDD-coated bSi needles were tested for their bactericidal properties.This was because literature reports on other structured surfaces suggest that spiky features of length B1 mm are optimal for cell destruction. 60,61everal 1 cm 2 of the BDD-coated short-needle bSi samples fabricated as above were laser cut into quarters to provide 10 identical samples each of size 0.25 cm Â 0.25 cm.These were then acid washed in concentrated sulfuric acid (495%, 10 ml, 80 1C) for 30 min and then reterminated with hydrogen using H 2 gas in a HFCVD reactor.This last step was to ensure that none of the oxygen termination that results from acid-cleaning diamond remained, as it is known that O-terminated diamond is an effective substrate for growth of many types of biological cells, 62 and thus is the opposite of what we require in this case.
Ten identical BDD films were deposited onto flat seeded Si samples to act as a control.The BDD was deposited using the same CVD conditions as before, except for a time of B8 h leading to a diamond film B4 mm thick.Due to the polycrystalline nature of the CVD process, the resulting diamond film was not perfectly flat, but was composed of facetted crystallites around 2 mm in size.
Each sample was then rinsed with absolute-ethanol before being submerged in 2 ml of a bacterial suspension (in a 12-well microtiter plate).The bacterium chosen was Pseudomonas aeruginosa, a common Gram-negative rod-shaped bacterium that is associated with disease in plants and animals including humans, such as pneumonia and various types of sepsis.It is currently of considerable medical importance because it is considered to be a prototypical multidrug resistant (MDR) pathogen with advanced antibiotic resistance. 63Bacteria culture preparation was conducted according to standard protocol. 64riefly, P. aeruginosa ATCC 27853 was grown aerobically for 16 h in 10 ml Luria-Bertani broth (LBB, Sigma-Aldrich) in a 37 1C shaker set at 220 rpm.The bacterial suspension was then diluted in LBB to OD 600 0.1 and further incubated until midexponential phase was reached.Bacterial cells were then harvested by centrifugation (7 min, 5000g), washed twice in 10 mM tris-HCl buffer (Sigma-Aldrich), and suspended in tris-HCl buffer to OD 600 0.3 (approximately 10 7 cfu ml À1 ).
The samples were incubated in the bacterial suspension for 1 h at 37 1C under static conditions before being rinsed with tris-HCl buffer to remove any non-adherent bacteria.1 ml of Live/Dead s BacLightt bacterial viability stain (ThermoFisher Scientific) was then applied to each surface (as per manufacturers' instructions) followed by incubation at 25 1C for 15 min, followed by a final rinse using tris-HCl buffer.The prepared samples were then maintained in 1 ml of tris-HCl buffer whilst fluorescence microscopy was used to visualize the bacterial viability.This viability stain simultaneously stained viable cells with calcein-AM (green fluorescence) and dead cells with propidium iodide (red fluorescence) solutions.Viable cells took up the lipophilic SYTO 9 and metabolized it into the fluorescent calcein, resulting in green fluorescence from only viable cells.In contrast, propidium iodide struggled to transpose a viable cell membrane and instead could only pass through the more disordered regions of a dead cell membrane, resulting in red fluorescence only in dead cells.ImageJ Software was then used to manually count the number of greenand red-stained cells and thereby quantify the number of bacterial cells with an intact cell membrane (viable) and a damaged cell membrane (dead).A two-tailed homoscedastic Student's t-test was performed to compare data sets.If the p value was less than 0.05, then results were statistically significant.
For SEM imaging, following incubation with P. aeruginosa, surfaces were fixed by immersion in a 2.5% v/v glutaraldehyde solution (Sigma Aldrich) dissolved in 0.1 M phosphate buffer (potassium phosphate monobasic and potassium phosphate dibasic, pH 7.2, Sigma Aldrich) for 2 h at room temperature.An alcohol dehydration stage was then performed by immersing the surfaces in 20%, then 40%, 60%, 80% and 100% ethanol for 10 min each prior to 10 min in hexamethyldisilazane (Sigma Aldrich).Surfaces were then air dried, mounted onto carbon stubs and sputtered twice with gold for 1 min each time.A Helios Nanolab 600 combined with FIB-SEM was used to visualize the surfaces.

Material characterisation
Laser Raman spectra of the BDD MCD coated substrates showed the characteristic peaks associated with heavily borondoped thin diamond films, namely the graphitic D and G bands from the sp 2 carbon at grain boundaries, the small, broadened diamond peak which is shifted and asymmetric due to Fano resonance, 2 and peaks at 500 cm À1 and B1200 cm À1 related to boron incorporation (see Fig. 3).The Si 1st-order peak at B520 cm À1 is obscured by the large B 2 feature, but the second order can be seen as a broad feature around 950 cm À1 .

Electrochemical performance
Cyclic voltammograms were first recorded only in the presence of background electrolyte solution (KNO 3 ), recording the capacitive current at a range of scan rates.The CVs recorded for the different electrode types all appeared quasi-rectangular in shape (see ESI †) characteristic of interfacial double-layer (DL) charging. 65,66The DL charging current is proportional to the active surface area of the electrode.There is significant difference in the capacitive current of the various BDD electrodes compared with that from the flat sample (see Table 1).This is not surprising, as DL charging is most apparent in systems with a large surface-area-to-volume ratio, as in materials with structures on the scale of mm to nm. 67 The largest DL capacitive current was produced by the partially overgrown BDD-coated long needles, followed by the BDD-coated long needles, and finally the BDD-coated short needles whose current differed negligibly from that of the flat BDD electrode.There was no significant difference in behaviour between MCD and NCD-coated electrodes of either type.Therefore, we concluded that the surface topology of the electrodes is more important than the minor differences in grain size and sp 2 carbon content between MCD and NCD.Assuming that for the flat diamond substrate the effective surface area and the geometric surface area were approximately equal, the effective surface area of each nanostructured electrode was then calculated from the ratio of the capacitances (also shown in Table 1).
The performance of the electrodes was then analysed in the presence of 1 mM K 3 [Fe(CN) 6 ], which resulted in CVs with the characteristic oxidation and reduction peaks as shown in Fig. 4. The peak-to-peak potential separation (DE p ) and the peak current density appear to be dependent on the diamond microstructure.As discussed in previous papers, 30,68 these two parameters exhibit a complex dependence on the electrode microstructure, particularly in the case of high-surface-area electrodes.For instance, the decrease in DE p with increasing electrode roughness reflects a decrease in the phenomenological charge-transfer resistance associated with electron transfer events.This could be connected to the increase in the effective surface area or to active sites exhibiting faster transfer kinetics.In the case that the dimensions of the electrode porosity are comparable to the diffusion layer thickness, DE p will decrease below the 59 mV associated with planar diffusion profiles.On the other hand, the increase in the peak current density with increasing roughness is primarily due to the increase in the background capacitive current and, to a lesser extent, to a decrease in the charge-transfer resistance.It is important to clarify that the peak current density for outer-sphere redox probes is determined by the geometric rather than the specific surface area.The reversibility of the redox probe is determined by the square-root dependence of the peak current with the potential scan rate as illustrated in Fig. 4(b).A more detailed analysis of the dynamic electrochemical responses is beyond the scope of this work.However, the data illustrated in Fig. 4 demonstrate the excellent charge transport properties in these complex diamond electrodes and their suitability for electroanalysis.
The two most sensitive electrodes, the BDD-coated and partially overgrown bSi long-needle samples, were then used for analysis of DA in the presence of UA.Fig. 5(a) shows CVs for DA in buffer solution for the 2 BDD-coated bSi electrodes compared to the flat BDD control.The high-surface-area electrodes lead to a sharp increase in the current response and a shift of the anodic current peak potential towards less positive values with respect to the flat BDD electrode.As opposed to ferrocyanide, DA requires adsorption to the electrode surface prior to the oxidation step (i.e. an inner-sphere electron transfer).Consequently, changes in the effective surface area will have a more significant impact on the phenomenological charge-transfer resistance and the voltammetric responses.Again, in the case of the bSi long needle electrode, the changes in the electrochemical kinetics are rather stark, leading to a reversible electrochemical response at a wide range of potential scan rates (Fig. 5(b)).
The analytical sensitivity of the two BDD electrodes toward DA voltammetric sensing was tested by the construction of analytical curves obtained by linear sweep voltammetry (LSV).Fig. 6(a) shows an example of LSVs recorded for different DA concentrations, in this case using the BDD-coated bSi long-needle electrode.The linear response to concentration can be seen in Fig. 6(b) for the three electrodes in question, along with their  relative sensitivities.The following values of analytical sensitivity were recorded: 0.21 A cm À2 M À1 (flat BDD); 0.52 A cm À2 M À1 (BDD-coated bSi long needles) and 0.57 A cm À2 M À1 (partially overgrown BDD-coated bSi long needles).Therefore, the analytical sensitivity increased significantly with the use of the microstructured BDD electrodes, and the best analytical sensitivity was recorded using the partially overgrown BDD-coated bSi long-needle electrode.Voltammetric testing of the performance of the electrodes towards UA detection was also undertaken, with broadly similar results to those from DA.The data and CV curves for the UA analysis can be found in the ESI.† For the simultaneous voltammetric sensing of DA and UA, differential pulse voltammetry (DPV) was selected as a more sensitive voltammetric technique.Initially, the possible interference of one analyte on the response of the other was evaluated.For this, analytical curves were constructed maintaining one of the analytes at a fixed concentration.Fig. 7(a) shows the DPVs obtained in the presence of UA at fixed concentration (3.0 Â 10 À5 M) and DA with concentration ranging from 3.0 Â 10 À7 to 5.0 Â 10 À6 M. The DA response was linear over a wide concentration range in the presence of UA (inset of Fig. 7(a)), and the peak current densities recorded for UA showed a low relative standard deviation of 1.02%.This result proves that DA sensing did not interfere with the UA response.The same test was performed maintaining DA at fixed concentration of 3.0 Â 10 À6 M while varying the UA concentration from 3.0 Â 10 À6 M to 5.0 Â 10 À5 M, as shown in Fig. 7(b).Again, the electrode response toward UA was linear in the presence of DA (inset of Fig. 7(b)), and DA peak current densities showed a RSD of 5.82%, demonstrating the non-interference of UA sensing on the DA response.Next, the simultaneous analytical curves for DA and UA were constructed using the BDD-coated bSi long-needle electrode.2. Thus, high analytical sensitivities and low LODs were achieved using the BDD-coated bSi long-needle electrode as an electrochemical sensor.The DPVs were repeated several times to determine the repeatability of the system.For this, ten successive DPV measurements (n = 10) were conducted on a buffer solution containing DA and UA at two different  For the analytes at the lower end of their concentration ranges, the RSD obtained for peak current densities was B0.65% for DA and B1.1% for UA, while at the higher end of their concentration ranges the RSD reduced to B0.51% for DA and B0.53% for UA, respectively.These results demonstrate the high precision of measurement and stability of response of the proposed BDD electrodes.

Bactericidal properties
Turning now to the bactericidal properties of the structured BDD surface, both the BDD surface and a control 'flat' surface (actually a facetted surface with 2 mm crystallites, as described previously) were exposed to high numbers of exponential phase P. aeruginosa for 1 h and their bactericidal effects assessed using Live/Dead staining.Typical fluorescence micrographs are shown in Fig. 9, in which cells with intact membranes are stained in green while cells with damaged membranes are stained red.The nanopatterned BDD surface showed a significantly higher (p o 0.001) proportion of membrane-damaged cells with very few red cells present on the control surface.Both control and nanopatterned surfaces tested found comparable numbers of cells (p 4 0.05) (Fig. 10(a)) and the percentage of stained dead cells is shown in Fig. 10(b).The nanopatterned BDD surface resulted in significant bacterial killing compared to the control surface.The overall number of dead cells on the control was B2%, compared to B13% on the BDD-coated short needle sample.Because both types of sample had diamond coatings with identical boron-doping levels and crystallinity, the enhanced cell death rate must be primarily due to the needle-shaped structured surface.Indeed, the increased cell death rate on the BDD-coated needle samples is comparable to that reported on other nanostructured antibacterial surfaces. 69iability staining was also coupled with SEM imaging where equivalent samples were fixed after 1 h bacterial adhesion, as shown in Fig. 11.On the 'flat' BDD-coated substrates, cells adhered horizontally and appeared to remain in a healthy state (turgid), correlating with results from fluorescence microscopy experiments.This is in contrast to the BDD-coated bSi shortneedles where a proportion of the cells appeared to be damaged by the nanofeatures, appearing flat and non-turgid.Thus, the greater non-uniformity of the surface appeared to effectively  damage a higher percentage of the bacterial cells consistent with the results from the Live/Dead assays.

D. Conclusions
We have demonstrated that coating bSi needles with BDD produces electrodes that have a high surface area which imparts high sensitivity to detection and measurement of analytes in aqueous solutions.Because the electrode active surface is diamond, all the usual advantages of diamond electrodes also apply to these electrodes -they are hardwearing and robust, have a large potential window, are resistant to fouling, etc., and these benefits are now combined with the economic advantages of being relatively cheap and easy to fabricate.Electrochemical data show that underlying charge transport properties of the microstructured diamond electrodes are not affected by the length of the needles, opening the possibility of fabricating microstructured diamond electrode arrays on bSi needles with lengths greater than 15 mm.On the other hand, the partially overgrown long needles exhibited the highest effective surface area, even if electron micrographs suggest partial coalescence of the diamond deposit.This behaviour suggests that short diamond deposition times lead to discontinuous coating and electrically isolated segments of the electrode.
The typical double-layer capacitance values for most carbon electrodes are of the order of 5 mF cm À2 , 70 whereas the BDDcoated long bSi needles had capacitance values of 280 mF cm À2 while the partially overgrown ones had values B640 mF cm À2 .Such high capacitance values might make these electrodes suitable for use in supercapacitor applications.Moreover, the fact that the capacitance is a function of the bSi needle length suggests that the electrode capacitance can be tuned for different applications by simply changing the etch time used to create the bSi needles.
We showed that these electrodes can be used to distinguish DA in the presence of excess UA, with sensitivities and LOD values consistent with values from other nanostructured electrodes. 54The BDD-coated bSi short needles proved to have good anti-bacterial properties, killing around 13% of the cells that were placed upon this surface after only 1 h incubation.This cell-death rate is comparable to those from other nanostructured surfaces 57,64 and to uncoated bSi. 55However, the advantage of the BDD coating is that it makes the surface far more robust and scratch-resistant.Sharp bSi needles can easily be scraped off the Si surface, and may then float in the air where they might be inhaled -with possible unwanted (and so far, unknown) health implications.This is far less likely with a diamond-coated bSi surface due to the hardness of the coating.
In this study, we only tested the short bSi needles for bactericidal properties.Having shown a positive result, the next steps will be to test diamond-coated bSi needles with a range of different lengths to determine the optimal needle length for maximum cell death, while maintaining a scratchresistant surface.Although the focus of the report has been on bSi, it is clear that diamond-coating any micro-or nanostructured surface could be a useful way to protect that surface from mechanical damage while retaining its required abilities.Thus, we might envision a range of other otherwise delicate nanostructured surfaces and materials optimised for, say, antibacterial properties, for which a protective thin diamond coating could be the key to their wide-scale use.

Fig. 1
Fig. 1 The two types of bSi before coating with diamond.(a) Short needles with length 0.5-1 mm.(b-d) Long needles with length 15-20 mm.

Fig. 2
Fig. 2 The two types of bSi after coating with BDD.(a) Short needles -the BDD-coated needles are more rounded than before.(b and c) Long needles -conformally coated with boron-doped MCD.(d and e) Long needles -conformally coated with smoother, more rounded boron-doped NCD.(f and g) The long needles are partially overgrown with boron-doped NCD, but there are still porous cavities allowing access to the interior of the film.(h) A 'flat' commercial BDD electrode used as a control.

Fig. 3
Fig.3Raman spectra of the BDD-coated long-needle sample using green (514 nm) excitation.Label key: Dia is the diamond peak, D & G are the disordered and ordered sp 2 carbon bands, respectively, Si is the second-order peak from the bSi substrate, B and B 2 are peaks due to boron and boron-dimer incorporation, respectively.

Fig. 4
Fig. 4 (a) CVs recorded in 0.1 M KCl solution containing 1 mM K 3 [Fe(CN) 6 ] at a fixed scan rate of 50 mV s À1 using electrodes made from commercial flat BDD, BDD-coated bSi long needles and partially overgrown BDD-coated bSi long needles.(b) An example of a set of 13 CVs recorded at different scan rates (1: 10 mV s À1 to 13: 400 mV s À1 ), in this case for the partially overgrown long BDD-coated bSi needles.Inset: Plots of anodic and cathodic peak current densities vs. v 1/2 .

Fig. 5
Fig. 5 (a) CVs recorded in 0.2 M phosphate buffer solution (pH = 7.0) for 2.0 Â 10 À4 M DA using flat BDD, BDD-coated long needles, and partially overgrown BDD-coated long needles.Potential scan rate = 50 mV s À1 .(b) An example of a set of CVs recorded at 13 different scan rates (1: 10 mV s À1 to 13: 400 mV s À1 ) in the same buffer solution as before containing 2.0 Â 10 À4 M of DA.This data set was recorded using the BDD-coated bSi long needle electrode.

Fig. 8 (
a) shows DPVs with both DA and UA present and while the concentrations of both are varied.The oxidation peaks are well separated and easily distinguishable over a large concentration range of both the two analytes.Fig. 8(b) and (c) show linear responses with concentration for DA and UA, respectively.From the analytical curves shown in Fig. 8(b) and (c), the analytical parameters for the simultaneous voltammetric determination of DA and UA were recorded, and these are given in Table

Fig. 11
Fig. 11 Example SEM images of P. aeruginosa after 1 h incubation on (a) 'flat' BDD surface and (b) BDD-coated short-needle surface.Image (a) shows healthy (turgid) bacterial cells, whereas image (b) shows damaged cells.

Table 1
Capacitance and effective electroactive surface areas for each electrode relative to that of the commercial flat BDD sample