Structural switching in self-assembled metal–ligand helicate complexes via ligand-centered reactions

Ligand centered reactions are capable of conferring structural switching between a metastable, self-assembled Fe–iminopyridine aggregate and a stable M2L3 helicate.

Allostery is a dominant mechanism of structural control in biosystems. 1 A molecular recognition event, or structural change at one position in a biomacromolecule causes multiple small changes to build up in the larger structure. This causes a change in conformation elsewhere in the system, oen activating or deactivating an enzyme by opening or closing an active site. 2 Synthetic chemists have mimicked this effect to create allosteric catalysts 3 and host molecules. 4 The concept of controlling structure via remote reaction or recognition processes has also been applied in the construction of molecular machines and switches. 5 Controlling these structural conversions requires the system to undergo a simple, predictable change in conformation upon a reaction in the body of the molecule. This eld is dominated by rotaxanes and catenanes, as large changes in structure can be induced by simple mild reactions, oen via redox 6 or acid/base processes. 7 Self-assembled metal-ligand cage complexes are an enticing target for this concept: by performing a reaction on the body of the cage, a change in ligand geometry could be induced that allows variation in host properties, guest release or other modes of analyte sensing. There are some elegant examples of this concept with metal-containing macrocycles, 8 and numerous groups have exploited guest induced transformations, 9 but simply conferring reactivity on a reversibly self-assembled cage complex is extremely challenging, 10 let alone applying that reactivity to confer structural changes. 11 Controlling the structure and assembly properties of complex, reversible selfassembled cages via external stimulus can extend the applications of these already valuable systems. Here we show that small changes in the non-coordinating backbone portion of a coordinating ligand can have drastic changes in the self-assembly properties of the cage complex, and the reaction outcome can be driven by the self-assembly process.
We have previously showed that rigid ligands based on dibenzosuberone 10f,12 or uorenone scaffolds 13 can confer exquisite control on the structural and stereochemical outcomes of self-assembly. Small changes in backbone rigidity and variations in internal hydrogen-bonding have large effects on the selfassembly process, and can lead to self-sorting between similar ligand scaffolds, 12 as well as stereoselective discrimination between many different isomers upon assembly. 13 Most relevant to this discussion are the properties of substituted dibenzosuberone-based systems. Specically, a distinct hierarchy in the stability of self-assembled M 2 L 3 helicate structures is observed, dependent on the non-coordinating functional groups present on the ligand backbone (Fig. 1). 12 The helicates are formed by multi-component self-assembly between 3,7-diaminosuberone-based species such as ketone A or alcohol B with 2-formylpyridine (PyCHO) and Fe(ClO 4 ) 2 in acetonitrile solvent. Complete selectivity for homocomplex formation was observed when these ligands were assembled in competition with each other, and suberone helicate 1 was formed with complete selectivity in the presence of ligand B. 12 The signicant stabilization of 1 over the highly similar 2 suggested that this product could provide directing effects in a postsynthetic modication process.
The core diaminosuberol scaffold B is easily accessed in 3 steps from dibenzosuberone, 10f and provides the foundation for the introduction of reactive functional groups to the selfassembled system that allow for postsynthetic modication. The challenge in postsynthetic modication of reversibly formed cage structures is tailoring the reaction conditions so that the sensitive self-assembled complexes are not destroyed or undergo unwanted side reactions during the process. The presence of nucleophiles is the greatest limitation: the cationic Fe-iminopyridine centers are sensitive to coordinative displacement, and are intolerant to species as mild as bromide or hydroxide ion, as well as primary amines or hydride reducing agents.
The choice of postsynthetic reaction is key, as is the nature of the reactive internal functionality. We have synthesized a variety of functionalized ligand cores containing internal groups but many of these groups are either too reactive to remain intact upon the initial self-assembly process, disrupt the assembly (e.g. amines) or are unreactive under mild conditions (e.g. ethers, carbamates 10f ). An alkyl chloride at the internal site is an enticing target for reaction, as suberyl cation formation can be forced by treatment with Ag + cations, and the subsequent attack can be performed by weak, neutral nucleophiles that would not disrupt the Fe-iminopyridine assembly contacts.
Diamino suberyl chloride ligand C was synthesized in 64% yield from suberol B by selective protection of the amine groups, followed by chlorination and deprotection in conc. HCl. These conditions are mild enough that the reactive suberyl chloride group remains intact. Treatment of ligand C with 0.66 eq. Fe(ClO 4 ) 2 and 2 eq. PyCHO rapidly gave the characteristic purple color of an Fe-iminopyridine-based assembly product. However, NMR analysis of the product formed aer 24 h heating was not representative of the expected M 2 L 3 helicate (Fig. 2b). Instead of the usual sharp peaks observed in the 1 H spectrum of Fe-iminopyridine assemblies, 9,10,13a,14 broad mounds were observed throughout the aromatic and alkyl regions of the spectrum. Increased reaction time or temperature did not appreciably alter the obtained spectrum. No signicant spectral differences were observed upon acquisition at elevated temperature, suggesting that the broad peaks in the NMR are not due to paramagnetism, but that the self-assembled product is not a single discrete complex. The spectrum is consistent with other disordered assemblies we have previously seen upon either incomplete assembly of mixed diaminosuberone species, 12 or the untemplated assembly of diaminouorenone derivatives. 13a Additionally, peaks corresponding to the diamino suberyl chloride or 2-formylpyridine starting materials were absent, suggesting that chloride ligand C is unable to form the expected M 2 L 3 assembly, and instead forms an undened oligomeric aggregate ( Fig. 2a and b).
The lack of dened structure upon assembly of chloro ligand C is an advantage for testing reactivity: the metastable assembly provides a local minimum for postsynthetic modication, 14 one that is more easily converted to a cage/helicate complex than a more thermodynamically stable and rigid system. For example, undened uorenol-based Fe-iminopyridine aggregates can be converted to M 4 L 6 cages via anion templation. 13a To probe the reactivity of the chloro aggregate 3, a simple substitution reaction using water was attempted. One molar equivalent of both water and silver perchlorate was added to a solution of 3 in anhydrous CD 3 CN, and the sample heated in an NMR tube in air at 45 C for 20 h. Silver perchlorate was used to drive a dissociative substitution process, enabling the use of a weak nucleophile. Aer 8 h, peaks corresponding to a discrete cage complex can be observed amongst the broad mounds corresponding to assembly 3. By 16 h, the NMR spectrum sharpened considerably, and a single product predominated ( Fig. 3b and ESI †), with full completion observed at 20 h. Surprisingly, the observed peaks did not correspond to those for suberol helicate 2, the expected substitution product, but of ketone helicate 1, the product of both substitution and oxidation.
As can be seen from Fig. 3b and c, as well as in the ESI, † only one type of cage is formed in the reaction, as the broad peaks for the chloro aggregate 3 smoothly give way to sharp peaks for Fig. 1 (a) Self-assembly of 3,7-diaminodibenzosuberone A and 3,7diaminodibenzo-suberol B into helicates 1 and 2 respectively; structure of (b) 1 and (c) 2, as determined by X-ray diffraction analysis. 10f Fig. 2 (a) Synthesis of diamino suberyl chloride ligand C and selfassembly into disordered aggregate 3. 1 H NMR spectrum of (b) aggregate 3; (c) suberyl chloride ligand C (400 MHz, CD 3 CN, 298 K).
suberone helicate 1. There is no buildup of other sharp peaks that would be indicative of other cage formation (see below for an example of NMR spectra of stereoisomeric mixtures of helicate assemblies), be they suberol helicate 2, suberone/suberol/ suberyl chloride mixed heterocomplexes or other cage complexes of varying stoichiometries (e.g. M 4 L 6 tetrahedra).
The reaction requires elevated temperature: reaction at 23 C is extremely sluggish, and essentially no conversion is observed aer 36 h. The specic silver salt and solvent were unimportant: use of AgNO 3 , or reaction in DMSO gave identical products under similar conditions. Most notably, at no point were any peaks observed that would correspond to the fully self-assembled suberol helicate 2. As only one discrete species is observed in the reaction, the nature of the reactive intermediates is unclear. It is clear, however, that there is no appreciable concentration of self-assembled helicate structures other than the nal product (although small concentrations of intermediates must be present). The metastable chloro assembly 3 must give way to other assemblies, presumably containing suberol and/or suberone ligands, but only aer oxidation does the nal helicate 1 form. ESI-MS analysis of the mixture formed aer 12 h reaction (see ESI †) showed small traces of a mixed chloroketone ML 3 assembly, but the spectrum was dominated by cage 1 and its fragments.
The absence of the expected suberol helicate 2 in the presence of air is unusual: there are only mild oxidizing agents in the system (Ag ions, perchlorate ion and atmospheric oxygen), and the oxidation runs cleanly at only 45 C. Also, the Fe II centers are unaffected by the oxidation process. Ligandcentered redox processes on self-assembled cage complexes employing redox active metals as structural components are rare, and most cases of redox reactions on self-assembled cages naturally focus on metal-centered oxidations. 15 To shed light on this, we attempted the reaction in the absence of air. If 3 was heated at 45 C with AgClO 4 and H 2 O under a N 2 atmosphere, no reaction occurred aer 48 h. At 80 C, however, the reaction proceeded sluggishly to form suberol helicate 2. Only aer 60 h reux was complete conversion observed ( Fig. 3f and g). Notably, the air-mediated oxidation process only occurs on the metastable assembly. If suberol helicate 2 was reacted with AgClO 4 and H 2 O in air at 45 C, no reaction occurred. Heating at 80 C for 36 h also gave no conversion to suberone 1. Surprisingly, the suberol helicate was resistant to more stringent oxidative processes: heating 2 at 45 C or 80 C with Dess-Martin periodinane did not confer oxidation to suberone 1. The process does not appear to be acid-mediated, as addition of even stoichiometric amounts of acid to the reaction cause cage decomposition. Slight decomposition was observed, but the helicate remained mostly intact, and no Fe-based oxidation occurred. Evidently, reaction of 3 is facile, but once a favorable self-assembled cage is formed, the reactive carbon center of the system becomes "locked", and resistant to further reaction. The metastable assembly 3 is "primed" for reaction, whereas the helicates are not.
Both the metastable reactant assembly and thermodynamically favored helicate product are essential for optimal reaction. As an illustration of this, control experiments were performed with stable ligand surrogate, 3,7-diacetamidosuberylchloride 6. Control 6 was chosen to mimic the electronic nature of the constituent amino-pyridine ligand of 3, while preventing amine or imine-based solvolyses or other unwanted side reactions. Chloride 6 was highly resistant to dissolution in acetonitrile, so the control reactions were performed in DMSO-d 6 , a solvent that also allowed smooth conversion of 3 to suberone 1. When 6 was exposed to the same conditions that gave complete oxidation of 3 to helicate 1 (i.e. 1 eq. AgClO 4 and 1 eq. H 2 O, heating at 45 C for 24 h), no reaction was observed. Only when the temperature was increased to 80 C and the reaction time increased to 36 h did any new products form, and in this case, only the alcohol substitution product 7 formed: no evidence of any oxidation to ketone was seen, even under these harsher conditions (Fig. 4). The substitution reaction proceeds normally with or without air. These conditions are similar to those needed for the air-free substitution reaction of 3, indicating that the accelerated oxidation reaction of 3 in air is funneled to the most stable suberone product, which provides a directing effect on the reaction outcome. Other tests were performed with control ligand 6 (see ESI †). In the absence of silver (employing Bu 4 NClO 4 instead) or the absence of ClO 4 À anion (using AgNO 3 ), no reaction was observed, even aer extensive heating.
Tests performed under strictly anhydrous conditions also (unsurprisingly) gave no reaction. Notably, even in the presence of added Fe II salts, the main product was the alcohol rather than the ketone. The metastable aggregate 3 is essential for reactivity, but is also somewhat unique: we have made numerous other diamino suberyl scaffolds that each form M 2 L 3 helices upon multicomponent self-assembly. To investigate the reasons why ligand C is incapable of forming a stable self-assembled cage, and to shed light on the driving forces for the reactive behavior of 3, we synthesized two more analogs of ligand C that mimic the size and reactive nature of the chloro group in C. Mesylate ligand D was synthesized from alcohol ligand B via the same protection/ activation/deprotection route as C. This provides a leaving group to the central scaffold, but one that cannot be forced towards cation formation via the application of Ag + ions. Tri-uoroethylether ligand E was synthesized directly from B via treatment with acid in triuoroethanol, and provides a less reactive, yet sterically similar internal group.
Our initial explanation for the uncontrolled assembly of C was the lack of hydrogen bonding groups in the central core. Suberol cage 2 exhibits a preference for a single (all-in) isomer at the prochiral CHOH stereocenters, likely due to self-complementary hydrogen bonding upon assembly. 10f Both ligands D and E have no H-bonding groups, but smoothly form M 2 L 3 helicate structures. Unlike the alcohol cage 2, no stereocontrol was observed in the assembly. The spectral data for tri-uoroether cage 5 is shown in Fig. 5, and illustrate the type of spectra observed for isomerically impure M 2 L 3 helicates. More peaks were present than would be expected for a single isomer, however the peaks are all sharp and well-dened, unlike the spectra seen for 3. DOSY analysis shows that the multiple peaks present in the 1 H NMR all belong to one species of the same size. 19 F NMR for the triuoroether cage shows several triplets indicating that the triuoroether group is subjected to several different environments from the various isomeric combinations of orienting the group in and out of the cage. Mesylate cage 4 also showed no stereocontrol upon assembly, and gave similar mixtures of isomers in its NMR spectra. The similarity in structure between C, D and E is incongruous with their large differences in assembly properties. Attempts to synthesize the analogous bromo-and iodo-suberyl core analogs were unsuccessful due to the reactivity of the doubly benzylic halides, so we turned to computational analysis. The structure of each possible isomer of the M 2 L 3 helicate complexes that could be formed upon self-assembly of ligand C, Fe(ClO 4 ) 2 and PyCHO was optimized using dispersion-corrected density functional theory (at the uB97X-D/6-31G* level, see ESI † for details), and the energies were compared to that of suberone complex 1. The calculated energy of each isomer of the chloride cage was signicantly higher than that of 1, ranging from approximately +10 kcal mol À1 for the "all-out" isomer, to +30 kcal mol À1 for the "all-in" isomer. The M 2 L 3 helicates have very small cavities, and are highly sensitive to interactions between large atoms and the other ligand backbones in the assembly. The structure optimizations indicated that the Cl atom is too large to allow assembly due to steric clashes with the other ligand backbones. Evidently, smaller atoms such as O are tolerable, and the extra substituents in 4 and 5 can point away from the core, allowing smooth M 2 L 3 formation.
Chloride abstraction and cation formation on the ligand is essential for the reactivity of 3, and neither mesylate cage 4 nor triuoroether cage 5 showed any reactivity. When either 4 or 5 was heated at 45 C or 80 C with H 2 O and AgClO 4 , in either CD 3 CN or DMSO-d 6 , no change in the NMR spectra was observed aer 36 h. Neither the mesylate nor the triuoroethyl ether group can be abstracted by Ag + ions, and so an associative reaction would be required. The hindered nature of the central Fig. 4 1 H NMR spectra of control ligand 6, and after reaction with 1 eq. H 2 O and 1 eq. AgClO 4 at 80 C for 9 h and 36 h with conversion to ligand 7 (400 MHz, CD 3 CN, 298 K). carbon atom in helicates 4 and 5 evidently limits access to reactants, and only upon cation formation can reaction occur. Easier access to the central carbon atom, and the metastable nature of 3 are responsible for its unique behavior.
In conclusion, we have shown that ligand centered reactivity can confer structural switching between a metastable selfassembled aggregate species and a stable M 2 L 3 helicate structure. The outcome of this process is directed and accelerated by the stability of the nal product structure: suberone helicate 1 is formed preferentially under aerobic conditions, and the process employs atmospheric oxygen as the oxidant for ligand-centered reaction in the presence of multiple redox-active coordinating metal ions. In the absence of air, the switching process occurs more slowly, and the reaction is directed to the less stable product of simple substitution, suberol helicate 2. In this case, the reaction is stereocontrolled, and one isomer of product is formed, directed by internal hydrogen bonding. In the absence of any directing effects from self-assembly, no oxidation is observed, even under harsh conditions and the presence of air: the control ligand shows a preference for simple substitution, but the self-assembly directs both substitution and oxidation. The metastable nature of the initial aggregate species is essential for the reaction: while aggregate 3 is "primed" for reaction, other analogous, putatively reactive helicate structures are inert to both substitution and oxidation, as the selfassembly "locks" the system, preventing reactivity.