“Inverse” thermoresponse: heat-induced double-helix formation of an ethynylhelicene oligomer with tri(ethylene glycol) termini† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc04959h

Ethynylhelicene oligomers with TEG terminal groups showed a unique thermoresponse in aqueous solvents: double-helix formation upon heating and disaggregation upon cooling.


Introduction
Molecules form dimeric aggregates in solution upon cooling and disaggregate upon heating, 1-5 and such a thermoresponse is termed an "ordinary" thermoresponse in this study. DNA is a typical example of a biological molecule that exhibits the "ordinary" thermoresponse and forms double helices upon cooling and random coils upon heating. 6 A dimeric molecular aggregation A + A / A 2 is generally an exothermic process with a negative enthalpy change DH < 0, because A 2 is a structure with less internal energy or enthalpy than 2A. Entropy also decreases in dimeric aggregation, which shows a negative entropy change DS < 0, because the freedom in molecular motion is decreased in A 2 compared with 2A. The Gibbs free energy DG ¼ DH À TDS increases with an increase in temperature T, because of DH > 0. Consequently, the concentration of A 2 decreases upon heating, and the equilibrium moves toward dissociation to give 2A according to the equations DG ¼ ÀRT ln K and R ln K ¼ ÀDH/T + DS (Fig. 1a), where K and R are the equilibrium constant and gas constant, respectively. The "ordinary" thermoresponse is widely observed for molecular dimeric aggregates.
In contrast, molecules that exhibit the "inverse" thermoresponse in dimeric aggregation are also conceivable, for which heating induces aggregation and cooling induces disaggregation. Hypothetically, this phenomenon can occur in an endothermic process with a positive enthalpy change DH > 0, and the process is accompanied by an increase in freedom in molecular mobility with a positive entropy change DS > 0. Consequently, the equilibrium moves toward aggregation to form A 2 upon heating according to the equations DG ¼ ÀRT ln K and R ln K ¼ ÀDH/T + DS (Fig. 1b), because of ÀDH/T < 0. However, such a thermoresponse is counter-intuitive. The dimeric aggregation of synthetic molecules exhibiting the "inverse" thermoresponse, being a molecular-level phenomenon, has essentially not been observed. 7 The "ordinary" and "inverse" thermoresponses are complementary, and the development of the latter will largely broaden the use of thermoresponsive materials. Here, we report the synthesis of [(M)-D-4]-C 12 -TEG, which is an ethynylhelicene tetramer with tri(ethylene glycol) (TEG) groups at its termini (Scheme 1), and it has a notable "inverse" thermoresponse: [(M)-D-4]-C 12 -TEG aggregated to form a double helix upon heating and disaggregated to give a random coil upon cooling in an aqueous solvent mixture of acetone/water/triethylamine. A sharp transition between the double helix and the random coil occurred due to temperature changes. groups at both termini, was designed in this study to examine aggregation behavior in aqueous solvents. Compound 4 with a TEG moiety was obtained from benzoic acid 1 (ref. 8) in 2 steps, which was then connected to the termini of the ethynylhelicene tetramer (M)-D-4H 5e by Sonogashira coupling (Scheme 1).
[(M)-D-4]-C 12 -TEG showed the "ordinary" thermoresponse in organic solvents; it formed double helices upon cooling and disaggregated into random coils upon heating. Solutions of [(M)-D-4]-C 12 -TEG were heated at 60 C or 40 C for disaggregation then cooled, and their circular dichroism (CD) and UV-Vis spectra were obtained. The CD spectra in chloroform (5 Â 10 À4 M) at 40, 25, and 5 C showed weak Cotton effects mirror-imaged to the typical random-coil state of (P)-ethynylhelicene tetramers 5a (ESI Fig. S1a †). On the other hand, [(M)-D-4]-C 12 -TEG showed an intense CD as well as a hypochromic shi in the UV-Vis spectrum in triuoromethylbenzene (1 Â 10 À3 M) upon cooling to 5 and À10 C (ESI Fig. S1b †). The apparent molecular weight of the double helix obtained by vapor pressure osmometry (VPO) studies (triuoromethylbenzene, 40 C) above 1 Â 10 À3 M was twice as large as the calculated molecular weight of [(M)-D-4]-C 12 -TEG (3726.7) (ESI Fig. S2 and Table S2 †). The results indicated the formation of double helices of [(M)-D-4]-C 12 -TEG in tri-uoromethylbenzene. The reversible structural change between random coils and double helices was examined for D3 at 360 nm (triuoromethylbenzene, 1 Â 10 À3 M) by repeating the cycle of heating to 60 C and cooling to 5 C (ESI Fig. S1c †). D3 360 increased upon heating and decreased upon cooling: the "ordinary" thermoresponse was observed in the aromatic solvent.
[(M)-D-4]-C 12 -TEG was soluble in non-aromatic polar solvents such as acetone and ethyl acetate even at a concentration of 1 Â 10 À3 M, at which conventional ethynylhelicene oligomers 5 without TEG moieties were not soluble. The "ordinary" thermoresponse was observed in the polar solvents as well as in the aromatic solvent. A solution of [(M)-D-4]-C 12 -TEG in acetone (1 Â 10 À5 M) showed an increase in the CD intensity and a hypochromic shi in the UV-Vis spectrum upon cooling from 40 to À10 C (Fig. 2), which indicated the formation of double helices upon cooling. Dimeric aggregate formation in acetone was conrmed by VPO studies (acetone, 45 C, above 4 Â 10 À3 M) (ESI Fig. S3 and Table S3 †). The CD analysis at a low concentration of 1 Â 10 À5 M (À10 C) and at high concentrations of 5 Â 10 À4 M and 1 Â 10 À3 M (5 C) converged on the same spectrum with the D3 of À1.1 Â 10 3 cm À1 M À1 at a wavelength of 360 nm (ESI Fig. S4 †). It indicated that the spectrum was that of the equilibrium-shied state to double-helices containing practically no random coils in the solution, namely S-double-helix state. The average diameter determined by dynamic light scattering (DLS) in acetone (1 Â 10 À3 M) at 5 C was 5.2 nm (ESI Fig. S5a †), which was consistent with the VPO result showing dimeric aggregate formation, not polymolecular aggregate formation. At a lower concentration (1 Â 10 À4 M), particles of 3.8 nm and 0.76 nm in diameter were observed at 5 C, which corresponded to double helices and random coils, respectively (ESI Fig. S5b †). The increase of larger particles and the decrease of smaller particles upon cooling from 25 and 40 C are consistent with the formation of bimolecular double helices from monomeric random coils. In ethyl acetate, the "ordinary" thermoresponse was also observed in the CD and UV-Vis spectra (ethyl acetate, 1.0 Â 10 À3 M) (ESI Fig. S6 †).
Notably, the "inverse" thermoresponse of [(M)-D-4]-C 12 -TEG was observed in aqueous solvents. In a mixed solvent of acetone/water/triethylamine (1/2/1, v/v/v), intense Cotton effects (1 Â 10 À5 M) were observed at 40 C (Fig. 3a), which coincided with that for the S-double-helix state in acetone (ESI Fig. S7 †). Upon cooling, UV-Vis absorption increased and CD intensity decreased, which indicated disaggregation (Fig. 3a). An isosbestic point at 350 nm indicated an equilibrium between two states, the double helix and the random coil. The average diameters obtained by DLS in acetone/water/triethylamine (1/2/ 1, 1 Â 10 À5 M) were 3.7 nm, 1.7 nm, and 1.1 nm at 40 C, 25 C, and 5 C, respectively (Fig. 3b). ‡The diameter at 40 C coincided  with that of the double helix in acetone (ESI Fig. S5a †), for which dimeric aggregate formation was conrmed by VPO (ESI Fig. S3 †). The decrease in the diameter upon cooling is consistent with the disaggregation from a bimolecular double helix to a monomeric state. Thus, the "inverse" thermoresponse was observed for [(M)-D-4]-C 12 -TEG in acetone/water/triethylamine (1/2/1): the double helix was formed at 40 C and the random coil at 5 C. In addition, the "inverse" thermoresponse in this system was conrmed to be a molecular-level phenomenon in the dispersed state by DLS analysis; it was not caused by polymolecular aggregation. The spectra reversibly changed in response to changing temperature in a manner opposite to the "ordinary" thermoresponse (triuoromethylbenzene, 1 Â 10 À3 M) (Fig. 4).
The "inverse" thermoresponse was also observed at different concentrations. Temperature-dependent changes in CD and UV-Vis spectra similar to those at 1 Â 10 À5 M were observed at 5 Â 10 À6 M and 1.5 Â 10 À5 M ( Fig. S8a and b †). It is notable that dimeric aggregation occurred at these low concentrations. DLS analyses at these concentrations showed that the size of the aggregates were similar to those at 1 Â 10 À5 M ( Fig. S9a and b †), and the "inverse" thermoresponse at the molecular level was conrmed. The "inverse" thermoresponse was observed at a higher concentration such as 3 Â 10 À5 M as well, although polymolecular aggregates partially formed below 20 C, as indicated by CD and DLS (ESI Fig S8c and S9c †).
The CD spectra in acetone/water/triethylamine (1/2/1, 1 Â 10 À5 M) at 10 C and 5 C coincide, which show a convergence to a spectrum with a D3 of À3.1 Â 10 2 cm À1 M À1 at 360 nm. The CD spectra at different concentrations, 5 Â 10 À6 M and 1.5 Â 10 À5 M, also converge to the same spectra at 5 C (ESI Fig. S8a and b †). In the following discussions, the spectrum in acetone/ water/triethylamine (1/2/1, 1 Â 10 À5 M, 5 C) with a D3 of À3.1 Â 10 2 cm À1 M À1 at 360 nm is dened as the S aq -random-coil state, which is the equilibrium-shied state to random coils in aqueous media practically containing no double helices. It was noted that the CD spectra of the random-coil state in organic solvents (ESI Fig. S1a †) and the S aq -random-coil state in aqueous solvents (Fig. 3a) are slightly different, which may be due to a specic conformation of TEG moieties in aqueous media. 9 The "inverse" thermoresponse also occurred when the amount of acetone was changed to 0.6/2/1 and 0.8/2/1, keeping the concentration of [(M)-D-4]-C 12 -TEG at 1 Â 10 À5 M (ESI Fig. S10 †).
Note that [(M)-D-4]-C 12 -TEG provided an unprecedented example of dimeric aggregation with the "inverse" thermoresponse, which is in contrast to the other known synthetic double helices that exhibit the "ordinary" thermoresponse. 4,5 An examination of the solvents revealed the critical roles of water and triethylamine. In a mixed solvent of acetone/triethylamine (3/1, 1 Â 10 À5 M), the "ordinary" thermoresponse was observed: CD and UV-Vis spectra of the random-coil state were obtained at 40 C and 25 C. The spectra changed at 5 C, and those of partial double helices were obtained upon cooling to À10 C (ESI Fig. S11a †). The presence of molecular-level aggregates but not polymolecular aggregates was conrmed by DLS, which showed average diameters from 0.84 to 0.88 nm at 25 C (ESI Fig. S12 †). In acetone/water (3/1, 1 Â 10 À5 M), the system was opaque, spectra of the random-coil state were obtained by CD at 40, 25, 5, and À10 C (Fig. S11b), § which may be due to the formation of polymolecular aggregates of random coils. The results indicated that both water and triethylamine are necessary for the "inverse" thermoresponse of [(M)-D-4]-C 12 -TEG.
Variable-temperature 1 H NMR studies (acetone-d 6 /D 2 O/triethylamine-d 15 , 1/2/1, 2 Â 10 À4 M) were conducted to obtain insight into the thermoresponse of [(M)-D-4]-C 12 -TEG in aqueous solvents. Broad proton signals of the terminal TEG groups were observed between d 2.4-3.5 at 25 C (ESI Fig. S13 †). When the temperature was increased to 40 C, the signals of the TEG groups became sharper and increased in intensity. An upeld shi of the chemical shis of HDO signals was also observed. These results are consistent with the reported hydration/dehydration of poly(ethylene glycol) (PEG) groups. 10,11 Thermodynamic parameters of the double-helix formation with the "inverse" thermoresponse were experimentally determined using equilibrium constants K (Table S4 †) obtained from the CD D3 values at 360 nm in acetone/water/triethylamine (1/2/ 1, 1 Â 10 À5 M), DH ¼ +2.4 Â 10 2 kJ mol À1 and DS ¼ +9.2 Â 10 2 J mol À1 K À1 (ESI Fig. S14 †). Note that both DH and DS are positive and large. This contrasted with the "ordinary" thermoresponse of the dimeric aggregation of molecules giving negative DH and DS values, 1,2b,3,4c,d including conventional ethynylhelicene oligomers in organic solvents. 5b The positive DH and DS consequently induced an increase in the dimerization constant K upon heating according to the equation R ln K ¼ ÀDH/T + DS, which appeared as the "inverse" thermoresponse (Fig. 1b). The result validated the hypothesized discussion in the introduction.
A signicant change in the double helix/random coil ratio was observed in response to small temperature changes (Table S4 †). For example, the double helix/random coil ratios in acetone/water/triethylamine (1/2/1, 1 Â 10 À5 M) at 40 C and 10 C were estimated to be 90%/10% and 3%/97%, respectively. The large DH resulted in a substantial "inverse" thermoresponse. The positive DH and DS of the "inverse" thermoresponse are counter intuitive in the dimeric molecular aggregation but can be explained by the hydration/dehydration of the PEG moiety. Dehydration upon heating makes the PEG moiety hydrophobic, which reduces the thermodynamic stability of random coils in aqueous solvents (DH > 0) and promotes dimeric aggregation. When the PEG moieties are dehydrated, DS increases and overcomes the decrease in DS resulting from dimeric aggregation. It is known that PEG and oligo(ethylene glycol) (OEG) are hydrated in water below the temperature dened as the lower critical solution temperature (LSCT), and that dehydration upon heating enhances hydrophobic interactions. Then, polymolecular aggregation and precipitation occur to reduce the molecular surface area exposed to water. 11-14 Similar heatinduced aggregation 15 and self-assembly 16 related to the hydration/dehydration of biological peptide and protein molecules have also been reported.
It should be noted here that [(M)-D-4]-C 12 -TEG formed dimeric aggregates upon heating, not polymolecular aggregates, which is another unusual aspect of the "inverse" thermoresponse phenomenon in this system. Such dimeric aggregate formation of synthetic molecules has not been reported. The result reminds us of peptides and enzymes, 15,17 which control their activities by forming dimeric aggregates upon heating and disaggregating to monomers upon cooling. In our system, triethylamine is considered to play a crucial role in the formation of dimeric aggregates of [(M)-D-4]-C 12 -TEG. Triethylamine and water are known to form hydrogen-bonds at low temperatures, and microscopic phase separation occurs upon heating. 18 The resulting triethylamine domains incorporate dehydrated [(M)-D-4]-C 12 -TEG molecules (Fig. 5). Fewer polar environments made by organic solvents can promote the dimeric aggregate formation of ethynylhelicene oligomer moieties, which is enthalpically driven by p-p interactions, and does not induce polymolecular aggregation as a result of the hydrophobic interactions. It should be emphasized again that the "inverse" thermoresponse in dimeric aggregate formation shown in this study is a molecular-level phenomenon in the dispersed solution state and is different from the phenomenon in which polymolecular aggregates are formed by hydrophobic interactions above the LCST.

Synthesis of [(M)-D-4]-C 12 -TEG
Under an argon atmosphere, a mixture of 4 (45.0 mg, 0.0451 mmol), tris(dibenzylideneacetone)dipalladium(0) chloroform adduct (0.82 mg, 0.753 mmol), cuprous iodide (1.72 mg, 9.03 mmol), tris(2,4,6-trimethylphenyl)phosphine (1.75 mg, 4.52 mmol), triphenylphosphine (1.18 mg, 4.52 mmol), tetrabutylammonium iodide (22.2 mg, 0.0602 mmol), triethylamine (0.1 mL) and N,N-dimethylformamide (1.0 mL) was freezeevacuated four times in ask A. In ask B, a mixture of ethynylhelicene tetramer (M)-D-4H 5e (30.0 mg, 0.0151 mmol) in THF (1.0 mL) was freeze-evacuated four times, and the mixture was slowly added to ask A. The mixture was stirred at 45 C for 20 min. The reaction was quenched by adding saturated aqueous ammonium chloride, and the organic materials were extracted with ethyl acetate. The organic layer was washed with brine, and dried over sodium sulfate. The solvents were evaporated under reduced pressure, and separation by silica gel chromatography and recycling GPC gave [(M)-D-4]-C 12 (3)   solvents, polar non-aromatic solvents, and the aqueous solution of acetone/water/triethylamine. [(M)-D-4]-C 12 -TEG exhibited the "inverse" thermoresponse in acetone/water/triethylamine (1/2/1): [(M)-D-4]-C 12 -TEG aggregated and formed double helices upon heating and disaggregated to random coils upon cooling. The double helix/random coil ratio sharply and reversibly changed in response to thermal stimuli. This is an unprecedented molecular-level "inverse" thermoresponse, in which dimeric aggregates but not polymolecular aggregates are formed. Positive and large DH and DS values in the aggregation process were determined, which were explained by the dehydration of terminal TEG groups upon heating and the formation of triethylamine domains that promoted double-helix formation by p-p interactions.

Note added after first publication
This article replaces the version published on 22nd February 2016, which contained errors in the grant numbers reported in the acknowledgements section.