Catalytic discrimination between formyl groups in regio- and stereoselective intramolecular cross-aldol reactions

Catalytic discrimination between inequivalent formyl groups was achieved by virtue of the mild reactivity of an aniline-type acid–base catalyst for the regio-, diastereo-, and enantioselective intramolecular cross-aldol reactions.


Introduction
Catalytic discrimination among similarly reactive functional groups is a key to realizing unique and efficient chemo-and regioselective transformations of multi-functionalized molecules; 1 however, formidable challenges to such discrimination remain. Intramolecular cross-aldol reactions of enolizable unsymmetric dial 1 require catalytic discrimination between the formyl groups bearing similar reactivities (Fig. 1). Although the reaction provides special versatility toward the production of cyclic b-hydroxy aldehydes potentially found in prostaglandins 2 and nucleic acid medicines 3, control over this reaction is quite challenging due to the production of eight isomers from two regioisomers (from path A and B), including diastereomers (anti/syn), and enantiomers of each isomer (Fig. 1). Reaction selectively may only be achieved by controlling the diastereoand enantioselectivities, in addition to controlling the regioselectivity of the products (path A vs. path B). In the amine-catalyzed reactions based on an enamine mechanism, a high regioselectivity is expected only under conditions that favor precise discrimination by the amine catalyst between two enolizable formyl groups. Under such conditions, these groups may be individually and selectively converted to the enamine component and the carbonyl component (Fig. 1).
In addition to these intramolecular reactions, selective direct intermolecular cross-aldol reactions between enolizable aliphatic aldehydes have been achieved. 2,3 The slow addition of a donor or acceptor aldehyde using a syringe pump and/or the Fig. 1 Possible regio-and stereoisomeric products from the intramolecular cross-aldol reaction of an enolizable aliphatic dial, and potentially preparable bioactive compounds bearing carbo-and heterocycles.
addition of excess amounts of one aldehyde to the other are indispensable for obtaining a good yield of the desired crossaldol product in the case of a-unbranched aldehydes. 4 Because these techniques cannot be employed in the intramolecular reaction of 1, the success of the reaction relies purely on the potential of a catalyst to discriminate between formyl groups. For these reasons, intramolecular cross-aldol reactions are highly challenging transformations.
Herein, we describe the catalytic discrimination between formyl groups by aniline-type acid-base catalysts based on their distinct mild reactivities. This approach yielded the rst examples of regio-, diastereo-, and enantioselective intramolecular cross-aldol reactions of enolizable unsymmetric dials. 5

Results and discussion
We assumed that L-proline was too reactive to discriminate between formyl groups bearing similar reactivities ( Fig. 2A). Therefore, we hypothesized that an acid-base catalyst bearing a mildly reactive amine could be advantageous in discriminating between the different formyl groups. 6 Our focus centered on an aniline-type amine as an amine with one of the lowest reactivities (Fig. 2B). We were particularly interested in anilinetype axially chiral amino acids, 7 and therefore we prepared (R)-11 and (R)-12, 13, 14, which possessed tetrazole and sulfonamide groups as acidic moieties, respectively (Fig. 2C). 8 Although aniline derivatives have been frequently employed as organocatalysts toward iminium activation, 9 their application toward enamine catalysis has not received signicant attention due to their weak basic and nucleophilic properties. 10 Therefore, we initially evaluated the catalytic activities of these aniline catalysts by performing intramolecular enolexo-intramolecular aldol reaction of 1,6-hexanedial (15) ( Table 1). 11 To our delight, cat. (R)-11 exhibited sufficient catalytic activity toward the reactions. 12 In the presence of 5 mol% cat. (R)-11, the 5-membered ring-forming enolexo-intramolecular aldolization of 15 proceeded smoothly to afford anti-16 and syn-17 in DMSO. A subsequent Wittig olenation gave the corresponding anti-18 and syn-19 with a high enantioselectivity toward the syn isomer (93% ee), although the diastereoselectivity of the products was moderate (anti-18 : syn-19 ¼ 1 : 1.3) ( Table 1, entry 1). A survey of catalysts and solvents (see ESI †) identied the primary amine catalyst (R)-12a, which possessed a triic amide, as capable of affording anti-18 as the major product in a 58% yield with a high diastereo-(anti : syn ¼ 6.4 : 1) and enantioselectivity (87% ee) (entry 2). DMF and acetone were good solvents for this reaction and improved the ee of anti-18 (entries 3 and 4). Changing the solvent to THF increased the diastereo-and enantioselectivities as well as the chemical yield of anti-18, giving an anti : syn ratio of 15 : 1, an ee of 97%, and a 74% yield aer 36 h (entry 5). The catalyst (R)-13, which bore a p-Ns group, was also active in this reaction and yielded anti-18 in an 80% yield with an 87% ee in a high diastereoselectivity (anti : syn ¼ 16 : 1) (entry 6). The opposite diastereo-and moderate enantioselectivities obtained in the presence of L-proline 13 revealed that these axially chiral anilines provided a unique chiral environment suitable for the 1,6-dial (entry 7). The tertiary amine (R)-12c did not promote the reaction, suggesting that the aniline-type catalysts promoted the reaction via enamine catalysis. The catalytic activities listed in Table 1 veried that the anilines are useful organocatalysts, even in enamine catalysis.
DFT calculations using the model catalyst 20 and the dial 15 also supported the enamine mechanism, including the ratedetermining iminium-to-enamine transformation (see ESI †). The most stable transition state (TS) for the stereo-determining C-C bond-forming step explained the stereochemistry of anti-16 via C-C bond formation between the Si faces of the enamine and the formyl group (Fig. 3). The structural and electronic factors in the TS played a crucial role in controlling the stereoselectivity. The structurally favored conformation of the enamine and the C-C bond-forming moieties caused strong hydrogen bonds to form between the sulfonamide NH and the formyl carbonyl group, thereby facilitating a t into the chiral space and stabilizing the TS for (1R,2R)-16.
With effective catalysts in hand, we moved again to examine the cross-aldol reaction of 1a (Table 2). Although the reaction in the presence of cat. (R)-12a gave dehydrated 9 as the major product (see ESI †), the milder acidic (R)-13 afforded 3,4-disubstituted anti-5a (59%) as the major product in 89% ee with a high diastereoselectivity (anti-5a : syn-6 ¼ 12 : 1) and the concomitant formation of the regioisomer, 2,3-disubstituted anti-7 (8%) ( Table 2, entry 1). The regioselectivity of the reaction, (5a + 6 + 9) : (7 + 8), was found to be 8.0 : 1. This regioselectivity contrasts signicantly with the corresponding value associated with the L-proline catalyzed reaction (entry 6). This selectivity indicated that cat. (R)-13 discriminated between the different formyl groups, which could not be distinguished by L-proline, and converted the C(6)-formyl group into the enamine component and the C(1)-formyl group into the carbonyl component, as shown in Fig. 1 (path A). The reaction in DMF improved the diastereoselectivity to 18 : 1 and the enantioselectivity to 93% ee, although the regioselectivity decreased slightly (entry 2). During this survey, we found that 1a was labile in DMSO and gave the opposite regioisomeric adducts anti-7 and syn-8 in the absence of a catalyst (regioselectivity, (5a + 6 + 9) : (7 + 8) ¼ 1 : 8.8) (entry 7). These results revealed that cat. (R)-13 overcame the background reaction to predominantly yield anti-5a. We also tested the primary alkyl amine catalyst, L-isoleucine, which was successfully employed in the intermolecular cross-aldol reaction with an a-branched substrate (entry 5). 3j This catalyst predominantly gave anti-7 and syn-8; however, no signicant ee value was obtained in syn-8, and a diastereoselectivity (7 : 8 ¼ 1 : 1.2) similar to that of the background reaction (7 : 8 ¼ 1 : 1.1) (entry 7) suggested that this catalyst did not overcome the background reaction. The combination of aniline (21) and p-Ns aniline (22) also afforded anti-7 and 8 as the major products (entry 4). These results indicated that cooperative activation of the substrate by acidic and basic moieties in biaryl framework was required for regioselectivity toward anti-5a.
The yield of anti-5a was improved to 75% through elongation of the reaction time without decreasing the enantioselectivity  ( Table 3). The N-Alloc-, N-Cbz-, and N-Boc-protected dials 1b-1d were applicable in this reaction and afforded anti-5b-5d in 90% ee (Table 3). It should be noted that 5d was isolated as the sole product in 95% yield, suggesting that the formyl groups of 1d were perfectly distinguished by cat. (R)-13. Anti-5d is an intermediate to the phosphorylase inhibitor, ulodesine (3, Fig. 1). 14 The 6-membered ring-forming cross-aldol reactions were further examined under the conditions used for the 5membered ring formation ( Table 4). As expected, 3,4-anti disubstituted piperidine (24) was obtained from the reaction of N-Boc 1,7-dial (23) in the presence of 5 mol% cat. (R)-13, followed by successive NaBH 4 reduction with high regio-and diastereoselectivities, although the enantioselectivity was low (entry 1). Unlike the ve-membered ring formation, cat. (R)-12a did not promote dehydration and gave 24 as the major product, with a slightly better enantioselectivity (entry 2). Changing the solvent to THF and lowering the temperature to 0 C in the presence of cat. (R)-12a improved the enantioselectivity to 86% ee with excellent regioselectivity (entry 3). The high regioselectivity indicated that the formyl groups of 23 were distinguished by the catalyst, leading to a reaction from the enamine of the C(7)-formyl group. This reaction provided a promising tool for constructing a 3,4-anti disubstituted chiral piperidine, such as R199715 (4, Fig. 1), previously prepared from 24. 15 The formyl groups of 25 without a heteroatom were also discriminated well by cat. (R)-13 to afford cyclopentane, anti-26, regioselectively in 74% yield with 82% ee (Scheme 1). The absolute congurations of the major enantiomers of anti-5a for entries 1-3 were determined to be (3S,4S). c The relative stereochemistry of all isomers was determined. The absolute congurations are tentatively based on the assumption that both products were generated from the same enamine geometry for anti-5a. d 74% ee was observed. e 6% ee, >99% ee, 42% ee, were observed for 5a, 6, and 7, respectively. f The absolute congurations of the major enantiomers was determined to be (2R,3R).  The origin of the regioselectivity was explored by treating a mixture of the aldol-adducts anti-7 0 and syn-8 0 , the minor regioisomers, with cat. 13. The absence of a conversion to anti-5a 0 suggested that the regioselectivity was governed by kinetic factors (Fig. 4A). The rate-determining step of the reaction was revealed by the kinetic isotope effect (KIE) using the a-deuterated substrate 1a-D. The apparent primary KIE (k H /k D ¼ 3.4) indicated that the rate-determining step was associated with the enamine-forming step from the iminium intermediate, which involved C-H bond cleavage (Fig. 4B). 16 Therefore, the regioselectivity of the reaction was dened by the enamine-forming steps rather than by the C-C bond-forming step. Furthermore, the regioselectivity of the reaction decreased to 1.2 : 1 for (5a-D + 6-D) : (7-D + 8-D) from 8.0 : 1 for (5a + 6 + 9) : (7 + 8) ( Table 2, entry 1). This result indicated that the kinetics associated with the iminium-to-enamine intermediate affected the regioselectivity.
These experiments suggested that the regioselectivity was controlled by the kinetics of the enamine formation. Selective iminium formation at the C(6)-formyl group ([29] > [30]) of 1a-1d may have affected the kinetics associated primarily with the production of the major regioisomers (Fig. 5). The thermodynamic stability of 29 due to the iminium cation located two carbons away from the electron-withdrawing NR group may have contributed to the predominant formation of 29 relative to 30 during the equilibration step. The steric factor of the catalyst may also play a role in the preferential formation of the Scheme 1 Intramolecular cross-aldol reaction to a chiral cyclopentane. a The absolute configuration of anti-26 was determined to be (3R,4S). b Determined by the integration of the 1 H NMR signals in the presence of 1,3-dinitrobenzene as an internal standard.  sterically less congested 29 than 30. The regioselective formation of anti-26 from dial 25 bearing gem-diester group instead of the NR group might be mainly governed by the steric factor (Scheme 1). The mild reactivity of the aniline-type catalyst may have facilitated the discrimination between tiny electronic differences and/or steric circumstances of the C(1)-and C(6)formyl groups. 17

Conclusions
In summary, catalytic discrimination among formyl groups was achieved in the highly regio-, diastereo-, and enantioselective intramolecular cross-aldol reactions of enolizable 1,6-and 1,7dials. The key to realizing formyl group discrimination was the mild reactivity of the aniline-type acid-base catalysts, which led to excellent regioselectivity. Mechanistic investigations including kinetic isotope effect studies and reductive amination experiments revealed that the regioselectivity was controlled under the enamine-forming steps. The high accessibility to the chiral pyrrolidines and piperidines provided a prominent feature of this cross-aldol reaction. Further mechanistic investigations are currently underway.