Fast colorimetric screening for visible light photocatalytic oxidation and reduction reactions †

Fast screening accelerates the discovery and optimization of chemical reactions. Here, we present the parallel irradiation and evaluation of 96 visible light photocatalytic reactions in a microtiter plate. After completion, a chemical indicator is added, allowing the spectroscopic determination of the formed stoichiometric by-products. Their quantity correlates in many cases with the conversion of starting materials and yield of photochemical reaction products. We demonstrate the concept with known photooxidations of organic compounds by riboflavin tetraacetate (RFTA) and reproduce published results and gas chromatographic analyses by a colorimetric assay. Two new photocatalysts for the hydroxylation of boronic acids and new substrates for the photocatalytic generation of aryl radicals from aryl halides were identified. By screening a series of drug molecules containing aryl halides, new photochemical dehalogenation reactions were found. The presented methods enable laboratories lacking sophisticated high-throughput analytical instrumentation to perform parallel optimization and scope determination of photocatalytic oxidation and reduction reactions.


Introduction
The discovery of new reactions is of key importance in organic chemistry.For applications in synthesis, new transformations must be optimized and their scope and limitations defined.This typically requires the exhaustive systematic variation of many reaction parameters.Individual yield determination by product isolation or calibrated chromatographic methods, such as GC or HPLC, is laborious, slow and expensive.In recent years, organic chemists increasingly used advanced instrumentation and automatization to accelerate discovery and optimization steps 1,2 leading to the development of various high-throughput screening methods. 3,4The scope of techniques ranges from multidimensional directed or undirected screens for reactivity discovery, [5][6][7] over robustness 8 and functional group tolerance 9,10 assays to parallel reaction optimization protocols. 11,12These are usually performed in microvials or microfluidic systems. 13,14The bottleneck of such screening methods is typically the analysis of the reaction outcome, which often utilizes GC, 15 HPLC 16 and MS 5,6,17 techniques.Even though the analysis based on chromatographic separation of all compounds present in the reaction mixture and their identification and database correlation allows the most accurate and detailed evaluation of an experiment, such an approach is not available to all laboratories due to lack of equipment.We therefore present here a low-cost colorimetric screening as an alternative for the initial assessment of photoredox catalytic reactions.Naturally, less accurate analysis will provide less detailed evaluation results, but this limitation may be compensated by faster and broader application in laboratories lacking high-end instrumentation.
9][20][21][22][23][24][25][26][27][28] MacMillan showed with his approach of accelerated serendipity that by random high-throughput screening, new reactions can be discovered, and a mechanism-based screening method to accelerate discovery was recently reported by Glorius. 29A focused screening of radical photocatalysed methylations by DiRocco demonstrated reactions in microtiter plates linked with UPLC-MS for the optimization of solvent and catalyst combination. 30n alternative screening procedure that does not require high-end instrumentation may be based on a colorimetric assay. 31It is easy to perform and evaluate.Numerous indicators were developed for the specific detection of functional groups, [32][33][34][35][36] but this limits general applicability.However, many photoredox reactions need a sacrificial electron donor or acceptor to complete the catalytic cycle.During this process, a few typical by-products are formed.While the substrates and products of different reactions may vary in their properties and structure, the by-products often remain identical, e.g.reduced dioxygen species such as H 2 O 2 and O 2 ˙− or simply protons.8][39][40] We describe a simple colorimetric indicator method to detect and quantify byproducts of 96 photocatalytic reactions simultaneously (Fig. 1).The by-product formation in these oxidation or dehalogenation reactions correlates in many cases with the reaction conversion and even with the product yield.

Screening of photocatalytic oxidations
Air oxygen is a typical terminal oxidant in many photooxidation reactions generating hydrogen peroxide, superoxide anions or other reactive oxygen species.Mixtures of potassium iodide with starch give a strong blue colour when iodide is oxidized to iodine, which then forms a complex with amylose. 41,42Starch indicator was previously employed in screening methods, but usually as an indicator of produced iodine and not hydrogen peroxide (Fig. 2). 35,43he reaction is very sensitive and can detect amounts of hydrogen peroxide as small as 50 μM when optimized for a microtiter plate reader (see the ESI, † Fig. S2).Using a UV plate reader allows a quantitative readout, but even with the naked eye, successful reactions are easily identified (Fig. 3).Fresh indicator was used for every measurement.In order for the indicator to work properly, the pH needs to be acidic and water is added in the detection step if the photoreactions are performed in organic solvents (see the ESI, † Fig. S4).
The well-described photooxidation of benzyl alcohols and benzylamines to benzaldehydes by riboflavin tetraacetate (RFTA) was used to evaluate the screening method (Fig. 4). 45he photooxidation produces equimolar amounts of hydrogen peroxide when converting substrates to products. 46For fast screening, the reaction mixtures were only irradiated for 15 min; initial conversions produce sufficient hydrogen peroxide for reliable detection and reaction conditions are more defined at low conversions facilitating a relative comparison. 47 Three benzyl alcohols were oxidized to the corresponding benzaldehydes and the reaction mixtures from the microtiter plate were analysed by GC and simultaneously evaluated spectroscopically after indicator addition (Fig. 3).The product yield was estimated from the amount of released hydrogen peroxide determined using the KI-starch indicator by calibration.Both methods provided very similar results, showing that the amount of hydrogen peroxide reflects the product formation in this photooxidation.For the screening of different substrates, relative reactivities are more useful than absolute yields.Therefore, we normalized the obtained colorimetric response to the best converted substrate and correlated others by their relative reactivity.We expanded the scope of the screened benzyl alcohols.As expected from the literature, 45 electron-rich derivatives react best.Using published reaction conditions for the oxidation of benzylamines in the microtiter plate assay gave relative reactivities, which reflect the reported yields of completed reactions very well. 44his shows that indicator response based on initial rates of reactions qualitatively correlates with isolated reaction yields for these photooxidations.Substrates that only serve as electron donors for flavin, but are not converted into aldehydes, such as butylamine, can give stronger or weaker false positive results.§ A critical evaluation of the produced data is essential as for most indirect screening methods.In the photocatalytic transformation of arylboronic acids to aryl alcohols introduced by Zou et al., a photocatalytically produced superoxide radical is consumed in the reaction (Fig. 5). 480][51][52][53][54][55] The presence of reactive oxygen species in the mechanism inspired us to study this reaction in a screening approach with the goal of identifying other organic photocatalysts and further optimizing the reaction conditions (Fig. 5).We selected two catalysts with high oxidation potential: flavin RFTA and 9-mesityl-10methylacridinium perchlorate (ACR).First, we investigated different aliphatic amines as sacrificial electron donors.Triethylamine was identified as the donor with the highest photocatalytic production of superoxide radical anions.In the presence of boronic acid, the generated oxidant is consumed.The quantified amount of the photogenerated oxidant in the presence and in the absence of the boronic acid therefore indicates the reaction conversion.This difference was recorded for 6 commonly used solvents; acetonitrile provided the best results.For comparison of a reaction in different solvents, the indicator response was normalized (see the ESI, † Table S1).¶ The identified best conditions were then used for larger-scale, batch reactions of three substrates giv-ing comparable yields after 16 h of irradiation to the previously used ruthenium catalyst under reported conditions (Fig. 5). 48

Screening of photocatalytic reductions
Aryl halides have been photocatalytically transformed into aryl radicals, which can abstract a hydrogen atom yielding the corresponding dehalogenated product.Other reaction pathways are C-H arylation or addition to double bonds (Fig. 6). 57,58An interesting approach for such transformation was reported using dimeric gold complexes. 59All reactions produce the corresponding acid H-X as a stoichiometric byproduct. 60Although in many cases a base is used in excess to ensure high yields, pH changes may indicate the relative reactivity of different substrates or the best conditions.
We recently reported the visible light photoreduction of aryl halides, 60 using different perylene-3,4,9,10-bis-Ĳdicarboximide) (PDI) dyes as photocatalysts.The photocatalytic reaction of 4-bromoacetophenone with 1 eq. of DIPEA results in a slightly acidic reaction medium of approximately pH = 5.We therefore selected bromocresol green (BCG) as an appropriate indicator for this pH range.Reaction mixtures, in which photocatalytic dehalogenation proceeds, give a yellow colour after addition of BCG, while a blue colour is Fig. 4 Solutions containing RFTA (1 mol%) and the respective substrates were irradiated for 15 min in a microtiter plate (in MilliQ water with 4% DMSO).Conversion was evaluated using a KI-starch indicator or by GC analysis.The relative reactivity of benzylamines was compared with reported product yields. 44g. 5 Reaction condition optimization of RFTA and ACR as photocatalysts in the hydroxylation of boronic acids.The isolated product yield from batch reactions under optimized conditions for three boronic acids and reported reaction conditions for RuĲbpy) 3 catalyst after 16 h of irradiation (455 nm) are compared.Y axes represent relative reactivity.
observed for non-successful reaction trials (see the ESI, † Fig. S14).We correlated the relative change in absorbance at the indicator λ max absorption (617 nm) with the relative reactivity of the aryl halides investigated.All reactions were performed in DMF with 1 eq. of DIPEA using PDI, IrĲppy) 3 and Ru(bpy) 3 as photocatalysts under 470 nm irradiation (Fig. 6). 61n DMF solution, the aryl radical abstracts a proton from the solvent or the sacrificial electron donor DIPEA and the dehalogenation product is formed. 62Representative aryl halides were selected covering a wide range of reduction Fig. 6 Screening results of the reduction of aryl halides.The compounds are given in the order of increasing reduction potential (V vs. SCE). 56Reaction conditions: 1 h, 470 nm, DMF, 1 mM substrate, 1 eq.DIPEA, 1 mol% catalyst.GC comparison for selected compounds: 12 h, 455 nm, DMF, 20 mM substrate, 10 eq.DIPEA, 1 mol% catalyst (10% for PDI).a Full conversion, but no yield of the reduction product.Fig. 7 Screening results of the reduction of heteroaryl halides.Reaction conditions (1 h, 470 nm, DMF, 1 mM substrate, 1 eq.DIPEA, 1 mol% catalyst) and their comparison with GC measurement (12 h, 455 nm, DMF, 20 mM substrate, 10 eq.DIPEA, 1 mol% catalyst (10% for PDI)).Table 1 Screening of commercial drugs containing aryl halide groups for their possible photocatalytic dehalogenation: no response (-), moderate response (•)

PDI
Ir(ppy) 3 Ru(bpy This journal is © The Royal of Chemistry 2016 potentials to explore the substrate limits of the three photocatalysts. 56Compounds octadecyl bromide, 3-bromopyridine, 2-bromonaphthalene and 4-bromoanisole, which are very difficult to reduce, show only negligible colorimetric response (<10%).Their potentials are outside the available reduction potential of the investigated catalysts under the reaction conditions and no conversion can be expected.However, the compounds ranging from 9-bromoanthracene to 4-chlorobenzonitrile (Fig. 6) require reduction potentials matching the reducing power of the catalysts and produce a detectable colour change (>10%).No colour change is detected in the case of 2-bromonitrobenzene, even though it has a redox potential lower than −1 V vs. SCE; therefore, it should be reduced.Previous findings showed that 2-bromonitrobenzene is unable to produce the aryl radical, as the radical anion is very stable and back electron transfer is favoured. 56Conversion of 9-bromoanthracene to anthracene was monitored by UV-vis spectral changes (see the ESI, † Fig. S16).Selected reactions of the screening were repeated on a larger scale (10 eq.DIPEA, 455 nm) and the reaction outcome was monitored by GC.The yields of the dehalogenated products correlate well with the relative reactivity derived from the colorimetric screening.The quick assay reproduces the accessible substrate scope and limitation of the three catalysts for the photocatalytic reaction well.Next, a series of heteroaromatic halides were investigated for photocatalytic dehalogenation using the same procedure (Fig. 7).2-Chloropyrazine, 2-bromobenzophenone and 5-bromonicotinamide in combination with PDI or IrĲppy) 3 showed moderate conversion in the assay (>10%), which was con-firmed by GC, producing the corresponding dehalogenated compounds, although only in low to moderate yields (Fig. 8).
To test our reactivity screening method on more complex substrates, we selected fifteen commercially available drugs (structures available in the ESI †) bearing an aryl halide moiety (Table 1).The compounds were screened for photocatalytic dehalogenation using the described assay.For three compounds, the acidification of the reaction mixture indicated a possible reactivity, and these were investigated individually on a larger scale.The indication for bromazepam was found to be a false positive; the compound does not convert into the expected product.In the case of hydrochlorothiazide and benzbromarone, we were able to isolate the dehalogenation products in moderate to good yield (Table 1, bottom) applying any of the three tested catalysts.This shows that our method is useful for an initial reactivity screening of more complex molecules with different functional groups.

Screening of photocatalyst stability
Crucial for all catalytic reactions is the stability of the catalyst.The photostability of the photocatalysts under the reaction conditions is essential to achieve good overall performance of the reaction.We investigated therefore the potential bleaching of several typical photocatalysts under different reaction conditions.The dyes were tested in different solvents with addition of a base (NaOH) or an acid (CF 3 -COOH) in air and in nitrogen atmosphere (Table 2, exact values see the ESI, † Table S3).The summarized stability results may serve as starting point for the selection of the appropriate photocatalyst under the given reaction conditions.

Conclusions
We have described and validated a simple high-throughput reactivity screening for photocatalytic reactions based on the colorimetric detection of by-products.Reactive oxygen species and pH changes were semi-quantitatively detected to monitor the reaction conversion of photooxidations and photodehalogenations. Using flavin photooxidations of benzyl alcohols and benzylamines as an example, the relative reactivities derived from the indicator response very well reflect the reported product yields for the respective substrate.For the photooxidative conversion of boronic acids into phenols, we identified two new photocatalysts by screening.As an example for photoreduction reactions, a colorimetric for aryl halide dehalogenation was developed, and the indicator results were confirmed by GC for several substrates including more complex drug molecules.The reported method may find use for facile initial screening of photocatalytic reactions and be a helpful tool in exploring reactivity or optimization without the need for sophisticated instrumentation.Using the described approach for by-product detection by an indicator enables laboratories with limited analytical capacities to perform rapid parallel screening based on UV measurements or visual inspection, which may accelerate the discovery and application of new photocatalytic transformation in synthesis.

Fig. 1
Fig. 1 Parallel screening of visible light photocatalytic reactions generating oxidative by-products or protons.S = starting material; P = product.

Fig. 3
Fig. 3 Picture of a microtiter plate experiment after irradiation and addition of iodine and starch as indicator.Blue color indicates the formation of peroxide.

Fig. 8
Fig. 8 Picture of a microtiter plate experiment after irradiation and addition of BCG indicator.A yellow color indicates increased acidity and hence reaction conversion.