A hybrid macrocyclic anion receptor exploiting the pyrrole-2,5-diacetamide unit

We report the synthesis of both macrocyclic and linear derivatives of pyrrole-2,5-diacetic bisamides, and the studies of their anion binding properties, which are further compared with the properties of similar derivatives bearing dipicolinic or isophthalic moieties. The new macrocyclic compound built on pyrrole-2,5-diacetic bisamide was found to be an efficient receptor for H2PO4 and PhCOO , even in DMSO-d6 + 0.5% H2O (v/v), with changed relative selectivity toward a series of anions with respect to its parental analogues. The anion binding properties of the linear derivatives of pyrrole-2,5-diacetic bisamide, in turn, were found to strongly depend on the pKa of the amide NH protons, which can be modulated through the introduction of various substituents.


Introduction
Anion recognition is a robustly developing branch of supramolecular chemistry, 1 owing to potential applications in the construction of molecular devices, such as sensors, responsive materials, extraction systems, simple and transmembrane transporters, transition metal catalysts and organocatalysts. 2 Various strategies exploiting weak interactions have been evaluated for achieving selective and efficient anion recognition. 3Among the interactions harvested for the receptor-anion binding, the hydrogen bond is arguably the most attractive. 4uch interactions are of relatively high energy and they can be readily multiplied using several H-bond donating groups.Finally, they can be easily introduced and manipulated by tailoring the structure of the putative receptor.Importantly, hydrogen bonding is spatially sensitive, as it is formed only if the H-bond donor is positioned accurately in respect to the H-bond acceptor.This feature allows for the design of receptors characterized by high selectivity for anions that match the size and the shape of the binding pocket.Consequently, hydrogen bonding has been broadly explored in studies of anion recognition with synthetic anion receptors. 5he hydrogen bond donating groups for this purpose frequently used include amide, sulfonamide, urea, hydrazide, hydrazine, and pyrrole moieties. 1Unlike the other groups, the pyrrole unit can act only as a donor, diminishing the risk of formation of intramolecular hydrogen bonding, unfavorable for anion binding. 6Consequently, pyrrole derivatives have attracted particular attention, with various linear and cyclic structures being explored as potential anion receptors. 7Alternatively, the pyrrole moiety has been used as a scaffold bearing additional hydrogen bond donors. 8This strategy has led to the formation of efficient and selective linear anion receptors, such as pyrrole-2,5-dicarboxyamides 9 and pyrrole-2,5-diacetamides. 10 The macrocyclic topology of putative receptors allows many H-bonding sites to be gathered in close proximity and arranged in a convergent manner, as required for effective host-guest interactions.Previously, we have shown that macrocyclic tetraamides, such as 1 and 2 (Fig. 1), are superior anion receptors over their linear analogues. 11We also found that the dipicolinic amide moiety, while itself interacting rather weakly with anionic guests, serves as a powerful unit in preorganizing hydrogen bonding sites. 12For instance, hybrid macrocycle 3, containing dipicolinic and isophthalic subunits, is a better anion receptor than both its parental congeners 1 and 2 (Fig. 1).We envisioned that introducing additional hydrogen bonding groups to such receptors may have the result of both strengthening the host-guest interaction and modulating the relative selectivity toward different anionic guest species.Here, we report on the synthesis and anion binding properties of hybrid receptor 4 which combines the potency of both dipicolinic amide and pyrrole-2,5-diacetamide building blocks, having a macrocyclic cavity of the same size as receptors 1-3 (20-membered ring).{
In addition, we prepared acyclic derivative 8a by simple amidation of diester 5 with n-butylamine, and also its 8b analogue by a two-step procedure (hydrolysis to diacid 9, followed by amidation with aniline and DIC) (Scheme 1).
Linear receptors 8a and 8b as well as dipicolinic acid bisamides (10a-b) (Scheme 1) were evaluated in terms of the relative contribution of the two aromatic moieties in receptor 4 (the dipicolinic amide and pyrrole-2,5-diacetamide building blocks) to its binding properties.Furthermore, this allowed the inuence of the macrocyclic topology of 4 on its anion binding properties to be estimated.Finally, we compared binding properties of receptors 4 and 11 (Fig. 2) which allowed us to abstract contribution of topological differences of hydrogen bond donors position and assess role of the pyrrole moiety.
The anion binding measurements of the putative receptors were carried out in DMSO-d 6 + 0.5% H 2 O (v/v), using the 1 H NMR titration technique, with appropriate TBA salts as sources of anions.The association constants (K a ) measured for 8a and 8b were substantially higher than those of dipicolinic bisamide 10, showing that the pyrrolediacetamide is a superior building block over the dipicolinic moiety for the construction of linear receptors (Table 1).Receptor 8b, bearing phenyl substituents instead of the n-butyl groups of 8a, binds anions with three-fold higher K a .
The macrocyclic topology of 4, by contrast, imposes a conformation suitable for the anion binding process, with all amide and pyrrole protons convergent.This structure led to a 70-fold higher K a for chlorides in comparison to K a measured for 8a (cf.Table 1).Binding to both benzoate and dihydrogen phosphate follows a similar tendency, with about one order of magnitude higher K a 's for macrocyclic 4 as compared with linear 8a.Interestingly, bromides are weakly bound by receptor 4, indicating that such macrocycles are not planar, but rather form bent conformations which allow interaction with larger guests.This is supported by crystal structure analysis carried out for complexes of analogous molecules, were diaromatic macrocycles oen form bent p-stacked structures.11b Comparison of the binding properties of macrocycle 4 with those of previously evaluated receptors 1-3 shows that the presence of the pyrrole unit modies the relative selectivity for binding of different anions (Table 1).Interestingly, the incorporation of the additional binding sitethe pyrrole NH protondoes not result in increased association constants of receptor 4. On the contrary, receptor 4 binds anions with about one order of magnitude lower K a 's than receptors 1-3.Hybrid dipicolinicisophtalic derivative 3 remains the most efficient receptor of this type.However, comparison of 4 with more structurally related macrocycle 11 11c shows that the additional hydrogen bonding NH group not only modies the relative selectivity but also leads to a substantial increase in the binding of the chloride anion, with a 10-fold higher K a .
Finally, in order to compare the binding properties of linear receptors 8 with the analogues obtained previously by Gale and coworkers, 10 we performed additional titrations in MeCN-d 3 (Fig. 3 and Table 2).As could be expected, 14 binding constants measured in acetonitrile-d 3 are higher than those measured in DMSO-d 6 .Compound 8b binds chlorides with the highest K a in this series of receptors.We found that the trend of increasing strength of the host-guest interaction follows the increasing acidity of the NH amide proton (dened by the chemical shis found in 1 H NMR spectra) in the order: alkyl, benzyl, and phenyl amide derivatives.Similar trends were observed in benzoate anion binding, which was bound the weakest by aliphatic derivative 8a, and the strongest by phenyl derivative 8b and 2-pyridylmethylene derivative 12c, with 4150 M À1 and 5270 M À1 association constants, respectively.
In order to conrm our rationalization of the linear binding system, we performed crystal structure analysis of receptor 8b.This showed that amide groups of the linear receptors are not coplanar with the pyrrole ring, but adopt the anti-anti conformation (Fig. 4).In consequence, contrary to its dipicolinic analogue (10), receptor 8b is not pre-organized for anion binding, hence the binding process requires a conformational change to adopt a pseudo syn-syn arrangement.

Summary
In conclusion, we have synthesized and studied the anion binding properties of both linear and macrocyclic receptors that incorporate the pyrrole-2,5-diacetamide building block.The linear receptors revealed relatively strong affinity for anions even in demanding solvents, e.g.DMSO + 0.5% H 2 O.The anion affinity of the linear pyrroleamide receptors strongly depends on the acidity of the amide NH protons, which can be controlled by different substitution of the side amide groups.The hybrid macrocyclic receptor constructed from both dipicolinic and pyrrole-2,5-diacetamidic building blocks showed superior affinity for anions as compared to the simple linear receptors, but inferior affinity as compared to the other hybrid receptors of this class.Overall, therefore, pyrrole-2,5-diacetamide further expands the repertoire of building blocks for the construction of linear and macrocyclic anion binding receptors.Fig. 3 Receptors used investigated by Gale and coworkers. 10ble 2 Stability constants (M À1 ) for the formation of 1 : 1 complexes of 8a-b, and 12a-d with various anions a