Issue 1, 2016

Metal induced self-assembly of designed V-shape protein into 2D wavy supramolecular nanostructure

Abstract

In order to understand and imitate the more complex bio-processes and fascinating functions in nature, protein self-assembly has been studied and has attracted more and more interest in recent years. Artificial self-assemblies of proteins have been constructed through many strategies. However, the design of complicated protein self-assemblies utilizing the special profile of building blocks remains a challenge. We herein report linear and 2D nanostructures constructed from a V shape SMAC protein and induced by metal coordination. Zigzag nanowires and wavy 2D nanostructures have been demonstrated by AFM and TEM. The zigzag nanowires can translate to a 2D nanostructure with an excess of metal ions, which reveals the step by step assembly process. Fluorescence and UV/Vis spectra have also been obtained to further study the mechanism and process of self-assembly. Upon the protein nanostructure, fluorescence resonance energy transfer (FRET) could also be detected using fluorescein modified proteins as building blocks. This article provides an approach for designing and controlling self-assembled protein nanostructures with a distinctive topological morphology.

Graphical abstract: Metal induced self-assembly of designed V-shape protein into 2D wavy supramolecular nanostructure

Supplementary files

Article information

Article type
Paper
Submitted
16 Sep 2015
Accepted
14 Nov 2015
First published
16 Nov 2015

Nanoscale, 2016,8, 333-341

Author version available

Metal induced self-assembly of designed V-shape protein into 2D wavy supramolecular nanostructure

S. P. Qiao, C. Lang, R. D. Wang, X. M. Li, T. F. Yan, T. Z. Pan, L. L. Zhao, X. T. Fan, X. Zhang, C. X. Hou, Q. Luo, J. Y. Xu and J. Q. Liu, Nanoscale, 2016, 8, 333 DOI: 10.1039/C5NR06378G

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