Issue 3, 2016

Bioorthogonal metabolic labelling with acyl-CoA reporters: targeting protein acylation

Abstract

Protein acylation is an abundant post-translational modification with a pivotal role in a plethora of biological processes. To date, metabolic labelling with functionalized precursors of acyl-CoA and subsequent bioorthogonal ligation to a complementary detection tag has offered an attractive approach for monitoring endogenous protein acylation with excellent selectivity. This review focuses on the applications of alkyne- and alkene-based bioorthogonal chemistries in the study of enzyme activity in vitro and summarizes the carboxylate-type chemical reporters that have enabled the visualization and identification of cellular acylated proteins. However, despite their importance, serious limitations question the use of this two-step labelling method in the quantification of the protein acylome.

Graphical abstract: Bioorthogonal metabolic labelling with acyl-CoA reporters: targeting protein acylation

Supplementary files

Article information

Article type
Review Article
Submitted
02 Oct 2015
Accepted
14 Nov 2015
First published
19 Nov 2015

Med. Chem. Commun., 2016,7, 399-408

Bioorthogonal metabolic labelling with acyl-CoA reporters: targeting protein acylation

Maria. E. Ourailidou, M. R. H. Zwinderman and F. J. Dekker, Med. Chem. Commun., 2016, 7, 399 DOI: 10.1039/C5MD00446B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements