Issue 11, 2016

Biochemical sensing by nanofluidic crystal in a confined space

Abstract

Electrokinetics at nanoscale has attracted broad attention as a promising conductivity based biochemical sensing principle with a good selectivity. The nanoparticle crystal, formed by self-assembling nanoparticles inside a microstructure, has been utilized to fulfill a nanoscale electrokinetics based biochemical sensing platform, named nanofluidic crystal in our previous works. This paper introduces a novel nanofluidic crystal scheme by packing nanoparticles inside a well-designed confined space to improve the device-to-device readout consistency. A pair of electrodes was patterned at the bottom of this tunnel-shaped confined space for ionic current recording. The readout from different chips (n = 16) varied within 8.4% under the same conditions, which guaranteed a self-calibration-free biochemical sensing. Biotin and Pb2+ were successfully detected by using nanofluidic crystal devices packed with streptavidin and DNAzyme modified nanoparticles, respectively. The limits of detection (LODs) were both 1 nM. This electrically readable nanofluidic crystal sensing approach may find applications in low cost and fast disease screening in limited resource environments.

Graphical abstract: Biochemical sensing by nanofluidic crystal in a confined space

  • This article is part of the themed collection: Optofluidics

Article information

Article type
Paper
Submitted
29 Mar 2016
Accepted
04 Apr 2016
First published
04 Apr 2016

Lab Chip, 2016,16, 2050-2058

Biochemical sensing by nanofluidic crystal in a confined space

W. Zhao, B. Wang and W. Wang, Lab Chip, 2016, 16, 2050 DOI: 10.1039/C6LC00416D

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