atography in speciation analysis of metal-containing biomolecules : a review

Monolithic supports are efficient stationary phases for most of the important chromatographic separation modes. They can be applied for the chromatographic separation of biomolecules, nanoparticles, proteins, peptides, nucleic acids and low molecular mass organic acids. At the industrial level, monolithic chromatography is frequently used for the purification of large plasmids and bacteriophages. Despite their high potential and simplicity of hyphenation to different mass spectrometric detectors, monoliths are rarely employed in elemental speciation analysis. The present overview extends over the applications of monolithic supports for chromatographic separation of a given chemical element species present in different biological samples where they were efficiently used as an alternative to commonly applied particle-packed chromatographic columns. The performances and applicability of monolithic supports in speciation analysis are discussed and the need for further investigations in this area emphasized.


Introduction
Metals play an essential role in living organisms. For understanding molecular mechanisms of metal-dependent life processes, it is necessary to study the interactions of metal ions and their species with genes, proteins, metabolites and other biomolecules within organisms and ecosystems. Hyphenated techniques that are used in speciation analysis typically combine a high resolution separation technique, like liquid or gas chromatography and gel or capillary electrophoresis, with selective and sensitive elemental (e.g. inductively coupled plasma mass spectrometry (ICP-MS)) or molecular mass spectrometric detectors (commonly with electrospray or matrixassisted laser desorption (MALDI) ionization). As a part of analytical methodology, they can importantly contribute to the information obtained from the experimental results thus extending the knowledge in the eld of metallomics. 1 The efficiency of separation signicantly contributes to the overall integrity of such information. The common tool used for the separation of metal-biomolecule complexes is different chromatographic techniques. Among recent developments and emerging directions in chromatography, monolithic stationary phases acquired a major importance in high-performance liquid chromatography (HPLC). 2,3 Monolithic chromatographic supports are made of a single piece of rigid porous material that is tightly sealed to the wall of a tube. They represent efficient stationary phases for most of the important chromatographic separation modes. The mobile phase ows through the pores of the separation medium at low back pressure, enabling the use of higher ow rates than those that are commonly applied in liquid chromatography. The resolution and capacity of monolithic chromatographic supports are independent of the ow rate. In general, the porosity of monolithic columns is much greater than that of particle packed columns. Convective mass transport enables fast and efficient chromatographic separation. Since monoliths represent a single body, they have no void volume. 4 In a book edited byŠvec, Tennikova and Deyl, a comprehensive review of the theoretical aspects of preparation, properties and separation using monolithic beds is provided, demonstrating also wide areas of their potential applications. 5 Monoliths are formed mostly from inorganic silica, 6 organic methacrylate based stationary phases 4,7-9 and poly(styrene-co-divinylbenzene) materials. 10 The aspects of polymer chemistry relevant to the synthesis of monolithic materials and metathesis polymerization-derived monolithic supports have been outlined by Buchmeiser. 10,11 Monoliths are available in disk, column and tube packages. 4 Nowadays, monolithic chromatography is widely used in industrial and preparative separation of biomolecules. [7][8][9] Poly(styrene-codivinylbenzene)-based monolithic columns have been successfully applied in the eld of proteomics for the rapid separation of peptides, proteins 12 and ribonucleic acids, 13 while the ringopening metathesis polymerization-derived monolithic anionexchangers were used for the analysis of double-stranded DNA fragments. [14][15][16] Furthermore, a successful application of the ring-opening metathesis polymerization-derived monolithic supports for their intended use as precolumns in capillary liquid chromatography for on-line removal of protamineoligonucleotide nanoparticles was reported by Gatschelhofer et al. 17 Methacrylate-based monolithic stationary phases also enable fast and efficient analyses of biomolecules and nanoparticles, 18 they have been applied in purication of large plasmids and bacteriophages, 19,20 and offer chromatographic separation of proteins, 21,22 peptides, 23 nucleic acids 4 and low molecular mass organic acids. 24 Furthermore, methacrylate based monoliths are progressively used in the eld of proteomics. [25][26][27][28] In spite of their high potential and simplicity in coupling to different detectors, monoliths were rarely used in elemental speciation analysis. 29 The main drawback was related to the use of high salt content in eluents, which is not desirable when ICP-MS is used as a detector. This also makes on-line combination of monolithic chromatography with electrospray ionization tandem mass spectrometry (ESI-MS-MS) difficult, resulting in the need for species structural characterisation. However, the recent advances in performances of ICP-MS instruments due to the development of the high matrix introduction (HMI) system allow direct analysis of samples with much higher levels of total dissolved solids than can be tolerated by conventional ICP-MS. Despite some disadvantages, there are several reports on the successful applications of monolithic chromatography in the determination of chemical species of elements. By the use of styrene-divinylbenzene co-polymer based monolithic stationary phases, chelating convective interaction media (CIM) monolithic disks were employed in a ow analysis system for the fractionation of labile copper (Cu) species in environmental water samples. 30 Complementary to particle packed columns, methacrylate-based CIM monolithic disks, bearing weak anionexchange diethylamine (DEAE) groups, were successfully applied in speciation analysis of zinc (Zn) in environmental 31 and chromium (Cr) in occupational health 32 samples. Monoliths are also prospective separation tools that may be used for the rapid separation of metal-biomolecule complexes. Nevertheless, only a few research groups have investigated the possibility for their implementation in the eld of metallomics. Pearson et al. 33 used reversed phase silica-based monolithic chromatography in the investigation of arsenic (As) urinary excretion aer ingestion of rice. Five As species were determined in rice and in urine by using an ion-pairing monolithic silica column coupled to ICP-MS. Other applications were related to investigations of metal-biomolecule complexes in human serum by the use of methacrylate-based monoliths. 34 In our group, as an alternative to the anion-exchange FPLC column, 35 a CIM DEAE monolithic disk 36 or column 37 was used for the speciation of aluminium (Al) in human serum. The possibility of the effective cleaning of the chromatographic supports with NaOH that appreciably reduced the column blanks 37 and the opportunity of coupling a large volume CIM column (8 mL) to the sensitive ICP-MS detector enabled the separation and quantication of Al bound to transferrin in human serum at physiological concentration levels. 37 Another successful application was the use of a 1 mL CIM DEAE-1 column in the study of the distribution of cisplatin in human serum. 38 In this work the performances of monolithic chromatography were compared to those of particle packed FPLC columns. The CIM monolithic column was found to be more robust and enabled analyses of more than 150 serum samples without cleaning of the column support. By the use of CIM DEAE disks, Hann et al. 39 developed a high throughput screening assay intended for mapping the metallodrugbiomolecule interaction. In the fully automated on-line 2D system combined with ICP-MS detection, sequential switching of the eluent from the SEC column to two parallel CIM disks enabled direct fractionation and speciation analysis of cisplatin in human serum. Hasegawa et al. 40 reported trace metal speciation in human serum based on the separation of human serum proteins by monolithic chromatography and trace metal mapping analysis. A CIM DEAE disk was employed for the separation of proteins, which was followed on-line by using a detection system that consisted of a UV absorption detector and ICP-MS. For the identication of the separated proteins, successive chromatographic fractions were collected and subjected to MALDI-TOF-MS analysis. The concept of monolithic chromatography was recently extended also to the use of an anion-exchange polymeric micro-monolithic column coupled with ICP-MS in the investigation of trace element speciation in human serum. 41 Applying the proposed method, species-specic differences between commercially available Seronorm L1 and L2 trace element reference materials were identied. Speciation analysis of heparinized plasma, EDTA plasma and serum from healthy volunteers revealed that EDTA strongly affects speciation of Fe and Zn, while the effect of lithiumheparin on the speciation of metals in human plasma was negligible. Another successful application of monolithic chromatography was the use of a CIM DEAE-1 monolithic column in speciation of Zn-citrate in human milk. 42 Quantitative separation of Zn-citrate complexes ([Zn(Cit)] À and [Zn(Cit) 2 ] 4À ) was carried out and the separated Zn species were determined "offline" by ame atomic absorption spectrometry (FAAS) or "online" by ICP-MS. In fractions collected under the chromatographic peaks, the Zn-binding ligand was identied by ESI-MS-MS. The results demonstrated that about 23% of the total Zn was present in the low molecular mass (LMM) milk fraction and that LMM-Zn corresponded to Zn-citrate. A CIM DEAE-1 monolithic column was also applied in the speciation of LMM-Ni complexes in tea infusions. 43 The column was hyphenated to ICP-MS and the ligands eluted in fractions which contained Ni identied "off-line" by hybrid quadrupole-time of ight mass spectrometry (Q-TOF MS). In white, green, oolong and black tea (Camellia sinensis) and owers of herbal chamomile (Matricaria chamomilla) infusions, Ni was found to be present in the chromatographic fraction in which quinic acid was identied by Q-TOF, while in the hibiscus (Hibiscus sabdariffa) tea infusion, the results of speciation analysis showed that Ni is present in its divalent ionic form. An innovative approach of the use of monolithic chromatography was the application of conjoint liquid chromatography (CLC) for simultaneous two-dimensional separation of ionic forms of metal-based chemotherapeutics from the portions bound to serum proteins. 44,45 The method is based on assembling CIM Protein G and CIM DEAE disks in a single housing forming a CLC monolithic column. On the rst disk the chemotherapeutic bound to immunoglobulin G is separated, while on the second disk the unbound form of the chemotherapeutic is separated from the portion bound to albumin and transferrin. In combination with UV and ICP-MS detection the kinetics of binding of Pt and Ru-based chemotherapeutics to serum proteins were investigated in spiked human serum. 44,45 The purpose of this review is to give an overview of recent successful applications of monolithic chromatography used for the separation of metal binding biomolecules in speciation analysis. The performances and advantages of monolithic supports are evaluated and the potential of their further use as a complementary tool to particle packed HPLC columns in the separation of metal-biomolecule complexes in different biological samples is estimated.
2. Monolithic chromatography as a promising tool for the separation of metal-biomolecule complexes Speciation analysis requires efficient and rapid separation of element species and their highly sensitive detection. In order to preserve the original species present in the sample and prevent any species conversion during analysis, fast separation is usually required. Monolithic chromatographic supports in general offer faster separation than traditional particle packed columns. So, recently monolithic chromatography is being gradually introduced, as a promising tool, for the separation of metal-biomolecule complexes. Stationary phases based on porous monolithic silica 33 or methacrylate-based monolithic supports [36][37][38][39][40][41] have been successfully applied in the eld of metallomics. These applications and characteristics of monolithic stationary phases are summarised in Table 1. The potential of the use of monolithic chromatography for the separation of metal-biomolecule complexes is evaluated in the following sections.

Silica-based monolithic supports for arsenic speciation
HPLC chromatography using a polymeric anion-exchange column Hamilton PRP X 100 was most frequently applied for the speciation of As(III), As(V), monomethylarsonate (MMA) and dimethylarsinate (DMA). 46 The separation of these As species was completed within 15 to 20 min. Silica based monolithic columns, in particular those modied for reversed-phase separations, have also appropriate characteristics for the speciation of As. Pearson et al. 33 investigated the potential of the use of ion-pair liquid chromatography for rapid separation of As species. For this purpose, they coupled a Chromolith reversed-phase monolithic silica column to ICP-MS. Tetrabutyl ammonium bromide (TBAB) was used as an ion-pairing reagent. In order to achieve selective and sensitive separation of As species, the composition and pH of the mobile phase were optimised (10 mM L À1 phosphate buffer containing 1.0% (v/v) methanol, pH 5.6). As presented in Fig. 1, under the optimal chromatographic conditions, fast and efficient separation of As(III), arsenobetaine (AsB), DMA, MMA and As(V) was obtained within 3 min. The procedure developed was applied to the speciation analysis of As in urine and food samples in an ingestion experiment designed to investigate the effect of rice consumption on As exposure in humans. Data of the urinary As excretion revealed that American long rice signicantly increases the excretion of DMA in human urine. In this work, the main advantage of monolithic chromatography over the use of conventional particle packed columns was the great reduction in the separation time.

Methacrylate-based monolithic supports for elemental speciation in biological samples
Methacrylate-based monoliths bearing ion-exchange functional groups are commercially available in disk, column and tube packages 4 and specially designed also as micro-columns. They possess excellent mechanical and chemical stability over a wide pH range and withstand the regeneration with NaOH. 47 Disks are the smallest monolithic columns. They are designed so that they t into a housing specially made for them. Up to four disks may be stacked together into a single CIM housing increasing the capacity of such monolithic supports, while its resolution remains unchanged. It is also possible to prepare a CIM conjoint monolithic column (CLC) by placing CIM disks with different active groups into a single housing. 44,45 In addition to small disks, larger CIM monolithic columns are also available as ready-to-use. Of different possible applications, separation of proteins still prevails among ion-exchange methacrylate-based monoliths. 4 In terms of separation power and capacity, CIM monolithic supports are advantageous over conventional chromatographic columns packed with particles. Their usage allows eluents to be applied during separation at high ow rates without any increase of back pressure, hence enabling fast separation and rapid regeneration of supports. They exhibit low nonspecic binding of biomolecules, resulting in excellent recoveries. CIM monolithic supports are also easy to handle, can be coupled to various detectors, and offer great potential for the separation of charged biomolecules. The additional benets are their robustness, long lifetime and relatively low cost.

Speciation of Al in human serum
The chemical speciation of Al in human serum is of crucial importance in assessing individual compounds that may contribute to Al neurotoxicity. Physiological concentrations of Al in human serum are very low, around 2 ng mL À1 . The complexity of the serum constituents, the low total concentration of Al, the high risk of contamination and the tendency of Al to be adsorbed by different chromatographic supports make speciation of Al in human serum a difficult task for analytical chemists. To obtain reliable analytical data in Al speciation, it is of extreme importance to prevent all possible sources of contamination. By the use of high density polyethylene or Teon laboratory ware and appropriate cleaning procedures to remove traces of Al from eluents, reagents, columns and ltering devices, speciation analyses were possible in spiked serum samples or in the serum of patients with elevated concentrations of Al. 48 For characterization and quantication of the Al-binding proteins in human serum, analytical procedures combining size-exclusion chromatography (SEC), anionexchange chromatography, UV and element specic detectors Table 1 Characteristics of monolithic supports applied in speciation analysis of metal-containing biomolecules   were usually applied, while identication of the Al binding ligand was performed by SDS-PAGE. 48 An anion-exchange FPLC Mono Q column was used by several researchers for the separation of Al species of the high molecular mass (HMM) serum fraction. 35,49 The results of these studies revealed that about 90% of Al in human serum was bound to Tf. Since there are no reference materials for speciation analysis of Al, the use of complementary analytical procedures is essential. As an alternative to ion-exchange FPLC columns, ionexchange CIM monolithic supports were complementarily applied. 36,37 Initial investigations indicated that the weak anionexchange CIM DEAE disk exhibits all the potential needed for its successful application. To prevent co-elution of low molecular mass (LMM) Al species with HMM-Al compounds on the CIM disk, serum proteins were rst separated from LMM-Al fraction by the use of SEC. A protein peak was collected (5.0 mL) and 0.1 mL of the sample aliquot injected onto the disk that was coupled on-line to UV and ICP-MS detection systems. As an eluent 1 M NH 4 Cl in 50 mM Tris-HCl and 30 mM NaHCO 3 buffer (pH 7.4) was used in gradient elution. Selective separation of IgG, Tf and albumin is obtained in 10 min. Al was separated at elution time characteristic of the Tf peak. The time of separation of serum proteins on the CIM disk (10 min) is shorter in comparison to that on the FPLC column (15 min). 35,49 The results demonstrated that the percentage of Al bound to Tf was 90 AE 3%. These data are in agreement with our previous observations 35 and observations of other researchers 49 who applied a particle packed Mono Q FPLC column in the speciation of Al. The developed CIM DEAE-ICP-MS speciation procedure enabled reliable determination of the concentration and composition of Al bound to proteins when the concentration of Al in serum was higher than 5 ng mL À1 . CIM disks enable easy manipulation during the cleaning procedure as well as simple coupling to ICP-MS. In order to lower the limits of detection that would enable the speciation of Al at physiological concentration levels in human serum, Murko et al. 37 applied the same chromatographic monolithic support and eluents using a larger 8 mL CIM DEAE-8 column. A larger column, with higher capacity enabled injection of a greater sample volume (1 mL). However, the limiting factor to achieve lower limits of detection was the extraneous contamination with Al. The ability of the CIM DEAE-8 column to sustain rigorous cleaning of the chromatographic support with NaOH and efficient cleaning of eluents used in the chromatographic procedure resulted in extremely low blanks (below 0.15 ng Al mL À1 ). By coupling the CIM DEAE-8 column to ICP-MS, speciation of Al was possible in human serum at normal physiological concentration levels from 0.5 to 2 ng Al mL À1 . A UV chromatogram for the separation of serum proteins and an overlay of Al elution proles of unspiked and spiked human serum aer pre-separation of the LMM-Al species by SEC are presented in Fig. 2. Data demonstrated that about 90% of Al in unspiked and spiked human serum was separated at the retention volume characteristic of Tf. The Al-binding ligand was further identied by Acquity ultra performance liquid chromatography-electrospray ionization mass spectrometry (UPLC-ESI-MS). The results again conrmed that the Al-binding serum protein is Tf. The data on the speciation of Al in serum at physiological concentration levels represent an important basis for studies of the Al distribution and its fate in the human body. CIM monolithic stationary phases can be recommended as a favourable separation support over particle packed FPLC columns for the speciation of Al-binding proteins in human serum. Due to its high capacity and the possibility of the effective cleaning that signicantly reduced the column blanks, the CIM DEAE-8 column enabled quantitative speciation analysis of Al at physiological concentration levels.

Speciation of essential trace elements in human serum
Speciation analysis of essential trace elements in human serum provides important information on the nutritional status of the body, and homeostatic mechanisms that regulate their transport processes, acute phase reactions, and protection against oxidative damage. Malavolta et al. 41 developed a fast method for the simultaneous speciation of iron (Fe), copper (Cu), zinc (Zn) and selenium (Se) in human serum by the use of monolithic anion-exchange micro-columns installed on a tandem HPLC system coupled on-line with an ICP-MS detector. The system used for the separation of serum proteins was set in tandem LC mode with off-line column regeneration. Two ProSwi SAX-1S polymethacrylate-based monolithic micro-columns (0.04 mL) with amine functional groups were switched through the ow manager valve back and forth between the analysis and the regeneration ow paths, thereby allowing off-line column regeneration and equilibration. While one column was equilibrated, the system injected the next sample onto the other column, shortening the time of analysis. As an eluent 1 M ammonium acetate in 10 mM Tris buffer (pH 7.4) was used in gradient elution. A representative example of chromatographic separation of Fe, Cu, Zn and Se species in human serum using a monolithic anion-exchange micro-column with ICP-MS detection is presented in Fig. 3. From this gure, it is evident that elements are eluted at different elution times. Based on overlapping of elution proles with standard human serum proteins, specic binding of elements was estimated, while quantication of individual element species was performed by peak area normalization and/or absolute quantication by calibration curves. Authors stated that despite three to six times faster analysis time in comparison to commonly applied chromatographic procedures, the resolution of separated element species was not compromised. The procedure developed was applied in speciation analysis of human plasma aer addition of different anticoagulant agents e.g. lithium-heparin or EDTA. As expected, EDTA strongly affected speciation, while almost no changes in speciation of Fe and Zn were seen in heparinised plasma. In their work authors observed also species specic differences in speciation analysis of Seronorm L1 and Seronorm L2 commercially available trace element reference materials.
Despite the very small amount of sample injected (1 mL) the reproducibility was acceptable for the most species investigated. The developed tandem micro-column chromatographic separation could represent a tool for fast screening of deviations in the trace element distribution in human serum initiated by various diseases or age-related changes.

Speciation of Zn-citrate in human milk
In human milk, zinc (Zn) is fractionated between high molecular mass fraction containing milk proteins and low molecular mass fraction where citrate was predicted to be the most important ligand for its complexation. It was demonstrated in numerous investigations that the bioavailability of Zn for infants is much higher in human than in any other type of animal milk consumed by humans. In order to quantitatively determine highly bioavailable Zn-citrate in human milk, authors applied speciation analysis, which consists of CIM DEAE monolithic chromatography, ame atomic absorption spectrometry (FAAS) or inductively coupled plasma mass spectrometry (ICP-MS) and hybrid quadruple time of ight mass spectrometry (Q-Tof Premier). 42 Speciation analysis of Zn in biological samples was a challenging task because at physiological pH values Zn is readily adsorbed on different chromatographic columns, interacts with numerous buffer solutions, and many of its complexes, including Zn-citrate, are moderately stable and hence, can be easily partially broken during separation. Method development started with the optimisation of instrumental parameters of chromatographic separation and spectrometric detection using synthetic solutions of Zn-citrate that was prepared in HEPES, MOPS or MES buffers, which do not interact with Zn. To separate Zn species 0.5 mL of the sample was injected onto a CIM DEAE-1 monolithic column and an aqueous -1.2 mol L À1 NH 4 NO 3 linear gradient elution applied for 10 min at a ow rate of 1.0 mL. Aer chromatographic separation, the concentration of Zn was determined either off-line in the collected fraction by ame atomic absorption spectrometry (FAAS) or on-line by inductively coupled plasma mass spectrometry (ICP-MS). The Zn-binding ligand was identied on the basis of the retention time and also by electrospray ionization tandem mass spectrometry (ESI-MS-MS). The analytical procedure developed enables reliable and quantitative speciation of Zn-citrate within the pH interval from 5 to 7, where [Zn(Cit)] À and [Zn(Cit) 2 ] 4À complexes coexist, with the limit of detection 0.01 and 0.0005 mg mL À1 of Zn-citrate (FAAS and ICP-MS, respectively). It was successfully applied in Zn speciation in human milk. The results revealed that in the LMM milk fraction Zn is bound exclusively to citrate.

Speciation of Ni in tea infusions
In this application of monolithic chromatography, complete speciation analysis, comprising the separation on a weak anion exchange DEAE-1 monolithic column, the detection by ICP-MS and the ligand identication by Q-TOF MS, was implemented with the aim to study the Ni speciation in tea infusions of black, green, white, red (dried leaves of Camellia sinensis), chamomile and hibiscus tea (dried owers of Matricaria chamomilla and Hibiscus sabdariffa, respectively). 43 Ni is a non-essential trace element, which if present in an average daily diet is considered as safe for healthy individuals. It's necessary only for individuals who already suffer from contact allergy to Ni and may develop systemic reactions from its dietary ingestion to keep dietary exposure to Ni under control. Prevailing sources of Ni in the human population are drinking water and different beverages with relatively high levels of Ni. Among such beverages are also various types of tea infusions.
In the study described, 43 samples of various dried tea leaves or owers with a median total concentration of 5.4 mg Ni kg À1 were analysed. Up to 85% of the total Ni was extracted with water during tea infusion preparation. Ni speciation analysis of tea infusion samples was carried out under the optimised chromatographic conditions. Separation of Ni species on the 1 mL weak anion-exchange monolithic column was completed in 10 min by applying aqueous 0.6 mol L À1 NH 4 NO 3 linear gradient elution with column recoveries close to 100%. Recoveries were lower when a strong ion-exchange support with quaternary amino groups was tested for the same purpose as it breaks the moderately stable Ni species present in tea infusions. Separation of Ni species was followed by ICP-MS monitoring m/z 60 and ligand identication by Q-TOF MS operating in high resolution and MS-MS modes. Data on Ni speciation in the samples analysed are presented in Fig. 4.
The main nding of this study was that with the exception of hibiscus tea, in all analysed samples of tea infusions, Ni was found to be mainly associated with quinic acid and not, as reported in the literature cited, with relatively high molecular mass polyphenols. Quinic acid was identied as the low molecular mass organic ligand with which Ni is complexed in all the tea infusions of slightly acidic pH (5.8 AE 0.2). In the hibiscus tea infusion, which had a pH of 2.7, Ni was found to be present in ionic divalent form, suggesting that its speciation in tea infusions is pH dependent. Authors concluded also that the speciation method developed is yet another example of the successful application of monolithic chromatography in speciation analysis and that new data on Ni speciation in tea infusions can be important in understanding its bioavailability, possible inuence on the quality of tea and perhaps also metabolic processes in plants, where quinic acid is an important natural metabolite.

Investigations of metallodrug-biomolecule interactions in human serum
CIM monolithic chromatography was implemented also for studying the interaction of metallodrugs with proteins in biological samples, which play a key role in the preclinical and clinical drug development.
Hann et al. 39 developed a two-dimensional fully automated system in which the intact proteins were separated in the rst dimension by SEC that was followed on-line by ion exchange chromatography (IC) employing two parallel CIM DEAE disks. Since covalent and non-covalent interactions are essential in this context, a native orthogonal separation was a prerequisite. The cycle time of separation using gradient elution with 1 M NaCl in 20 mM Tris-HCl (pH 7.4) and applying on-line ICP-MS detection was 3 min. While the rst disk was being eluted within 3 min, the other was being loaded from the SEC column. Sequential switching of the eluent from the SEC column to two parallel CIM disks enabled direct fractionation and speciation analysis of cisplatin in human serum. The elution prole of the protein was monitored by ICP-MS as SO + polyatomic species via the dynamic reaction cell technique. Separation of albumin and Tf cisplatin adducts on the CIM disk is presented in Fig. 5. The developed throughput screening assay for metallomics exhibits increased selectivity compared to one-dimensional LC-ICP-MS. By injecting only 20 mL of the sample, quantitative determination of anionic species with absolute limits of detection in a low pM range (regarding sulphur) is possible. An important advantage of the SEC Â IC setting in combination with ICP-MS elemental detection is the potential for mapping any candidate metallodrug interactions with proteins in biological samples.
Martinčič et al. 38 applied a CIM DEAE-1 (1 mL) monolithic column with higher capacity than the CIM disk in investigations of the distribution of cisplatin in human serum. First, rapid Pt fractionation was performed by SEC on a Hi-trap desalting column. A 0.1 mL aliquot of the protein fraction was then injected onto a CIM DEAE-1 column. By applying gradient elution with 1 M NH 4 Cl in 50 mM Tris-HCl and 30 mM NaHCO 3 (pH 7.4) and coupling the column on-line to UV and ICP-MS, interactions of cisplatin with albumin, Tf and IgG, or their mixture were studied. The protein peak was collected aer preseparation by SEC, or the sample was injected directly onto a CIM DEAE-1 column (Fig. 6). The results of fractionation revealed that more than 80% of Pt in serum was eluted with proteins and the remaining portion as unbound cisplatin. Cisplatin did not interact with IgG. It exhibited a small interaction with Tf, and was strongly bound to albumin. Experimental data proved that the fraction eluted under the IgG peak corresponds to unbound cisplatin, enabling direct speciation analysis of cisplatin in the serum of cancer patients who received cisplatin-based chemotherapy. In parallel to monolithic chromatography, Pt speciation was also performed by using a Mono Q FPLC particle packed column coupled to ICP-MS. Two complementary speciation procedures gave statistically comparable results. Good repeatability (RSD below 3%) of consecutive chromatographic separations of the spiked serum sample was obtained for both Pt speciation procedures. The limit of detection (LOD) for separated Pt species by applying conventional and monolithic chromatography was found to be 0.06 ng Pt mL À1 . The low LOD enabled speciation of Pt in the serum of cancer patients treated with cisplatin. In comparison to the CIM monolithic column, the particle packed FPLC column exhibited better resolving power. However, the CIM DEAE-1 column is an appropriate separation support for its intended use in speciation of Pt and possibly other metallodrugs in human serum. It was found to be more robust and enabled analyses of much larger series of serum samples. Due to its ability for regeneration and equilibration at high ow rates (10 mL min À1 ), efficient removal of retained serum constituents from the column support is possible, which contributed to extremely repeatable and reproducible separations. At least 150 successive serum separations could be performed before the regular cleaning of the column was necessary. This is a great advantage over the use of conventional particle packed FPLC columns.
Further important improvement of the analytical method for the speciation of Pt in human serum originating from anticancer drugs (cisplatin, oxaliplatin and carboplatin) was made by the same group by the introduction of conjoint liquid chromatography (CLC). 44 Two monolithic convective interaction media (CIM) disks namely, CIM protein G and CIM DEAE, were assembled together in a single housing forming a CLC monolithic column. This column was coupled on-line to UV and ICP-MS detectors. Such a set-up allows rapid two-dimensional separation by affinity and ion-exchange (IE) modes to be carried out in a single chromatographic run. Before two-dimensional chromatographic separation of Pt-species was carried out chromatographic conditions were optimised separately for the CIM protein G and DEAE disks. A CIM protein G disk was chosen to retain IgG and a CIM DEAE disk to separate unbound Pt species from those bound to HSA and Tf. By this twodimensional separation, Pt species were quantitatively eluted (column recoveries between 95 and 97%) on the CLC monolithic column within 13 min. At the rst min, elution was isocratic with Tris-HCl-NaHCO 3 buffer (pH 7.4) followed by gradient elution with 1 mol L À1 NH 4 Cl (pH 7.4) in the next 9 min and, in the last 3 min, nished with acetic acid (AcOH). During the rst 10 min IgG was efficiently retained on the CIM protein G disk, enabling selective separation of unbound Pt from Pt bound to Tf and HSA on the CIM DEAE disk, using NH 4 Cl (pH 7.4) as the eluent. The elution with AcOH which followed allowed further separation of Pt bound to IgG. Separated Pt species were quantied by the post-column isotope dilution (ID)-ICP-MS. Comparison of performances of the CLC method developed with those of common procedures that involve separation of protein peaks by size-exclusion chromatography (SEC) followed by IE separation of metal-based chemotherapeutic fractions bound to serum proteins revealed that the CLC method is much faster and simpler. It was experimentally proven that it had also adequate sensitivity (LOQs for the quantication of all separated Pt species were lower than 2.4 ng Pt mL À1 ), selectivity and method repeatability (RSD AE 3%), to be successfully applied in the investigation of the kinetics of the interaction of the Pt-based chemotherapeutics studied with serum proteins and the distribution of Pt species in spiked human serum. The results from such investigation indicated that cisplatin and oxaliplatin had high reactivity towards HSA but much lesser reactivity towards Tf and IgG. Around 80% of cisplatin was found to be associated with HSA, 2% with Tf and 4% with IgG. Approximately 70% of oxaliplatin was bound to HSA, 3% interacted with Tf and 11% with IgG. The proportion of unbound cisplatin and oxaliplatin was 15 and 18%, respectively. The reactivity of carboplatin in spiked serum was much lower. Aer 48 hours of incubation, about 49% of carboplatin remained in unbound form, around 47% was bound to HSA, 2% to Tf and 2% to IgG. The main conclusion from the research described was that the CLC based speciation method may be reliably applied in preclinical and clinical studies of the kinetics of the interaction and distribution of different metal-based drugs with proteins in blood serum. This hypothesis was further conrmed in the study on the speciation of Ru in human serum. 45 This study was a part of a pharmacological characterisation of a candidate Ru based drug. Chromatographic conditions were the same as for the separation of Pt species, protein elution was followed on-line with UV detection at 278 nm, and the separated Ru species were quantied by post-column ID-ICP-MS. The instrumental set-up enabled fast two-dimensional separation by affinity and ion-exchange modes to be carried out in a single chromatographic run. Two Ru-based chemotherapeutics were compared. A newly synthesised compound chlorido(h 6 -p-cymene)(nalidixicato-k 2 O,O)Ru(II) (1) and (H2im)[trans-Ru(III)Cl 4 (Him) 2 ] (2; KP418).
The CLC procedure applied was suitable for its intended use with good sensitivity (limit of detection (LOD) 0.027 mg Ru mL À1 for (1)) and good repeatability (RSD AE 3.5%). By applying CLCbased speciation analysis, investigation of the kinetics of interaction of positively charged and neutral complexes of Rubased drugs with serum proteins as well as their species distribution in human serum was possible (Fig. 7). Negatively charged Ru complexes, on the other hand, co-eluted with Tf and HSA and thus hindered their speciation analysis. The candidate Ru-based drug studied (1) was a neutral Ru complex that hydrolyses to a positively charged species. Therefore, it was possible to study its kinetics in human serum by the use of CLC-ICP-MS.

Speciation of serum proteins based on trace metal mapping analysis
Speciation of metals bound to proteins is one of the most challenging issues in the present metallomics research. Although a variety of hyphenated systems that combine highresolution separation techniques with highly sensitive and selective detectors have been developed, it is still difficult to identify individual metal-binding proteins due to the complexity of biological matrices. In support of these investigations Hasegawa 40 developed a multiply hyphenated system for the speciation of trace elements in human serum proteins based on trace metal mapping analysis. Separation of proteins was performed by injecting 20 mL of the sample onto a CIM DEAE disk using gradient elution with 1 M CH 3 COONH 4 in 20 mM Tris-HCl (pH 7.4). Under optimal conditions, representative serum proteins, e.g. g-globulin, albumin, ceruloplasmin, transferrin and a 2 -macroglobulin, were separated on the disk. These proteins, which were primarily assigned by UV absorption, were identied with on-line multielement detection by ICP-MS at m/z 65 for Cu, m/z 66 for Zn and m/z 57 for Fe. Each successive fraction was collected and proteins were further identied off-line by MALDI-TOF-MS analysis. Characteristic chromatograms of a human sample separated on the CIM disk followed by UV and ICP-MS detection are presented in Fig. 8. Although the chromatographic selectivity was rather poor and could be improved by careful optimization of the parameters that inuence the chromatographic separation, the developed multiply hyphenated HPLC/UV/ICP-MS system equipped with a fraction collector for subsequent MALDI-TOF-MS analysis represents great potential for further identication of different proteins in biological samples.

Conclusions and outlook
Monolithic supports are widely used in chromatography as an alternative to conventional HPLC for fast separation of biomolecules. Porous monolithic stationary-phases allow efficient mass-transport at low back pressures and excellent chromatographic separations. Despite their wide use and possibilities for rapid separation of ionic species and metalbiomolecule complexes, monolithic chromatography was rarely applied in elemental speciation analysis in the eld of metallomics.
Nevertheless, some successful applications in the analyses of different metal-complexes in various biological samples revealed that monolithic chromatographic supports can be employed as an efficient alternative to commonly used particle packed columns. So far, ion-pairing reversed phase chromatography using a silica based monolithic column was applied for fast and selective separation of ve arsenic species in the investigation of urinary As excretion aer ingestion of rice. Other applications were related to the use of methacrylatebased monolithic supports. Regarding the sample matrix and characteristics of elemental species, monolithic microcolumns, disks and larger 1 to 8 mL columns were applied and connected on-line with ICP-MS detection. In some studies, fractions separated on CIM supports were further subjected to mass spectrometric identication of the metal-binding complexes by ESI-MS or MALDI-TOF-MS. In investigations of Al speciation in the protein fraction of human serum, the use of a large capacity 8 mL CIM DEAE-8 column and the robustness of the stationary phase to withstand rigorous cleaning with NaOH, which consequently signicantly lower the column blanks, enabled quantitative speciation of Al at physiological concentration levels in the serum. Speciation of essential trace elements in human serum was also reported by applying a micro-anion exchange monolithic column on a tandem HPLC system coupled on-line with an ICP-MS detector. Furthermore, CIM DEAE methacrylate-based monolithic chromatography demonstrated high potential in investigations of metallodrugbiomolecule interactions in human serum. An original 2D fully automated system was developed where the intact proteins were rst separated on a SEC column. By sequential switching of the eluent from the SEC column to two parallel CIM monolithic disks coupled to ICP-MS, direct fractionation and rapid speciation analysis of cisplatin in human serum was possible. Employment of a larger CIM DEAE-1 mL column coupled to ICP-MS enables the investigation on interactions of cisplatin with serum proteins and its distribution in the serum of cancer patients who received cisplatin-based chemotherapy. The main advantage of the CIM DEAE-1 mL column over the use of a conventional particle packed FPLC column was that at least 150 successive serum separations could be performed before the regular cleaning of the column was necessary. CIM monolithic disks were successfully applied also in the separation of serum proteins based on trace metal mapping analysis. Fast separation at little back pressure offered possibilities to successfully use monolithic supports in Zn speciation analysis in human milk samples. Difficulties like preserving the integrity of Zn species during analysis and unwanted Zn interaction with various chromatographic columns or numerous buffer solutions were efficiently solved by the application of Zn speciation analysis, which consists of the chromatographic separation on a weak anion-exchange CIM DEAE-1 monolithic column, ICP-MS detection and ES-MS-MS ligand identication. Zn-citrate was found to be the only low molecular mass Zn species present in human milk. The previously published data on the presence of Zn-citrate in milk perfectly agreed with those quantitatively determined by monolithic chromatography. For Ni speciation in various tea infusions it was experimentally found that low molecular Ni-quinate complexes or ionic divalent forms of Ni (depending on the pH of infusion), rather than high molecular weight Ni polyphenolic species, as presumed in the literature, were present in the samples. Assembling two different monolithic disks in single housing and hence creating a chromatographic column that enables two-dimensional separation to be carried out in a single chromatographic run was a key improvement in the development of Pt or Ru speciation analysis suitable to be implemented in pharmacokinetics studies of different Pt or Ru-based chemotherapeutics human serum samples. A CIM protein G disk was chosen to retain IgG and a CIM DEAE disk to separate unbound Pt or Ru species from those bound to HSA and Tf. All the species analysed were quantitatively eluted on the CLC monolithic column within 13 min. The examples described here clearly suggest that monolithic chromatography due to its characteristics, like the speed of analysis, has great potential to be more frequently applied in speciation of metal-containing biomolecules. Rapidness is of great importance in order to preserve chemical species of elements during the separation step and in the investigations of the kinetics of binding of metallodrugs to target molecules. With information gathered, drugs with increased effectiveness, broader spectrum of activity and reduced side effects may be designed. The advantage of the monolithic chromatography lies also in the extreme robustness of monolithic supports that sustain rigorous cleaning, which results in low column blanks. The robust monolithic supports enable also the application of a much greater number of successive separations of serum samples, before the cleaning procedure is required, than is possible by the use of conventional chromatographic columns. With good repeatability (RSD around 3%) of consecutive chromatographic separations and comparable or even lower LODs, monolithic chromatography is an attractive alternative to particle packed columns. As a powerful separation tool, monolithic chromatography may be also introduced in the investigations of the role of metal ions in chronic neurodegenerative disorders like Alzheimer's and Parkinson's disease.
Furthermore, separation of charged low molecular mass metal complexes in a variety of biological samples would extend its applications in the eld of metallomics.