Hydrogen bonding-induced conformational change in a crystalline sugar derivative †

We report crystallographic evidence of the change of a regular chair conformation to a skew boat conformation in a partially protected sugar derivative. Crystallographic correlation with two more analogous derivatives indicates that a particular O – H ⋯ O hydrogen bonding network is responsible for the change in conformation.


Chemical synthesis
Tolyl-1-thio-β-D-galactopyranoside (1) was synthesized by following the literature procedure. 11It was reacted with dry acetone in the presence of H 2 SO 4 -silica to furnish the corresponding 3,4-O-isopropylidene derivative 2 in 85% yield.Selective protection of the primary OH group using benzoyl cyanide and Et 3 N gave the mono-benzoate derivative 3 in 89% yield (Scheme 1).All three derivatives were purified by flash chromatography using an n-hexane-EtOAc mixture in a suitable ratio and characterized by 1 H and 13 C NMR and mass spectrometry.
1 H and 13 C nuclear magnetic resonance (NMR) analyses of compounds 1, 2 and 3 were performed using a Bruker 500 MHz NMR at 298 K. CD 3 OD was used as NMR solvent for compound 1, whereas CDCl 3 was used for 2 and 3.

Mass spectrometry
The mass spectra were recorded on a Q-TOF Micro YA263 high resolution (Waters Corporation) mass spectrometer by positive-mode electrospray ionization.

Gelation and gel characterization
A required amount of the compound and a measured volume of the desired pure solvent were placed in a screw-capped vial with an internal diameter (i.d.) of 10 mm and were slowly heated till the solid was completely dissolved.The clear solution thus obtained was cooled to room temperature in air to form the gel.The vials were inverted to confirm the formation of the gel.The minimum gelation concentration (MGC) was determined by finding the minimum amount of compound required for gel formation at room temperature.The gels were found to be thermally reversible.Upon heating above their gel dissociation temperature, they transformed to solution state and returned to their original gel state upon cooling.

Determination of gel-sol transition temperature (T gel )
The gel-to-sol transition temperature (T gel ) was determined by the dropping ball method.A small glass ball (230 mg) was placed on 1 mL of gel in a standard 15 mm vial.The vial was heated slowly in a thermostated oil bath while observing the rising temperature carefully.The temperature at which the ball dropped to the bottom of the vial was recorded as T gel .

FT-IR spectroscopy
The FT-IR spectra of compounds 1, 2 and 3 in their crystalline state and compound 1 in its xerogel state were obtained using a Fourier-transform infrared spectrometer (PerkinElmer 502).KBr samples (2 mg in 20 mg of KBr) were prepared and 10 scans were collected at 4 cm −1 resolution for each sample.The spectra were measured at room temperature over the range of 4000-500 cm −1 .

Field emission scanning electron microscopy (FE-SEM)
The morphologies of the reported gels were investigated using field emission-scanning electron microscopy (FE-SEM).A small amount of gel/solution was placed on a clean microscope cover glass and then dried by slow evaporation.The material was then allowed to dry under vacuum at 30 °C for two days.The materials were gold-coated, and the micrographs were taken using a FE-SEM apparatus (Jeol Scanning Microscope JSM-6700F).

Rheological study
To understand the mechanical strengths of the swollen hydrogels (swollen in DI water overnight), we performed rheological measurements on a AR-G2 rheometer (TA Instrument) using a steel parallel plate geometry with a 40 mm diameter at 25 °C.The rheometer is attached to a Peltier circulator thermo cube that helps with accurate control of temperature during the experiment.The storage modulus (G′) and loss modulus (G″) of the polymer gels have been recorded in the linear viscoelastic regime at a strain of γ = 2% of the angular frequency (0.1-100 rad s −1 ).

Single crystal preparation
Crystals of the compounds 1, 2 and 3 were obtained for single crystal X-ray diffraction.Crystals of compound 1 were obtained by slow evaporation of a methanolic solution.Block-shaped colourless crystals appeared in seven days.In the case of galactoside 2, colourless needles were obtained after twenty five days from a dilute solution in 1,2dichlorobenzene.Compound 3 gave colourless needles after eight days upon slow evaporation of a solution of the compound in an n-hexane-ethyl acetate (1 : 1) mixture.

Crystallography
The single-crystal X-ray diffraction data of the crystals were collected on a SuperNova, Dual, Mo at zero, Eos diffractometer at 292 K for compound 1 and 100 K for compounds 2 and 3 using graphite-monochromatic Mo Kα radiation (λ = 0.71073 Å).Atomic coordinates, and isotropic and anisotropic displacement parameters of all the non-hydrogen atoms of the two compounds were refined using Olex2, 12 and the structure was solved with the Superflip 13 structure solution program using Charge Flipping and refined with the ShelXL 14 refinement package using least squares minimization.The structure graphics shown in the figures were created using the X-Seed software package version 2.0.and mercury software package version 3.5.

Melting point
The melting points of the three crystals were measured using a digital melting point apparatus, SECOR INDIA.The melting points for the crystals of compounds 1, 2 and 3 were found to be 115 °C, 54 °C and 113 °C, respectively.

Results and discussion
Compounds 1, 2 and 3 were tested for their ability to form gels in different solvents.The results of those experiments are summarized in Table 1.Only compound 1 showed significant gelation ability in bromobenzene, chlorobenzene, 1,2dichlorobenzene and 1,3-dichlorobenzene (Fig. 2) with a minimum gelation concentration (MGC) range between 0.35 and 1.2% w/v.1,3-Dichlorobenzene was found to be the best solvent when the MGC is concerned.The gels are thermoreversible in nature and no significant change in the gelation behaviour was observed upon several heating and cooling cycles.The gels were stable at room temperature and no noticeable change was observed when kept in a closed container for a couple of months.It shows the temporal stability of the gel.The effect of the concentration of the gelator on the gelto-sol transition temperature (T gel ) is shown in Fig. S1 (ESI †).
The T gel value increased up to 381 K with an increase in the gelator concentration of 6% w/v and remained almost constant till 9% w/v showing no further concentration dependence.

FT-IR study
Hydrogen bonding is one of most important interactions among the non-covalent interactions responsible for the formation of the supramolecular architecture in gel or crystalline state.The FT-IR study is particularly useful for the detection and characterization of hydrogen bonding in the selfassembled state.Fig. 3 shows the FT-IR spectra of the crystalline state of compounds 1, 2 and 3 and the xerogel state of 1.In all cases, the absence of any band at 3600 cm −1 (free OH   stretching vibration) clearly indicates that all the O-H groups are hydrogen bonded.Moreover, it is observed that the IR bands for the hydrogen bonded O-H groups are well resolved in the case of the crystalline states compared to the gel state.This suggests the involvement of the hydrogen bonding network towards the formation of the supramolecular architecture.

Microscopic studies
The microstructures of the gels formed by compound 1 in different solvents were inspected by FE-SEM to get the visual insight into their morphology.Fig. 4a-d show the FE-SEM images of the xerogels of compound 1 in 1,2-dichlorobenzene, 1,3-dichlorobenzene, bromobenzene and chlorobenzene, respectively.All of them show three-dimensional networks formed by cross-linking of self-assembled fibrillar networks (SAFiNs), 15 which are approximately 70-100 nm in diameter and several micrometers in length, indicating the entrapment of the solvent molecules into the spaces of the 3D network.

Rheological study (frequency sweep) of the gel
To judge the tolerance power of the gel, rheological measurements were done to determine the frequency sweep.Frequency sweep rheometric measurements were carried out using the gels (1.0%, wt/v) with 1,2-dichlorobenzene, 1,3dichlorobenzene, bromobenzene and chlorobenzene at a low shear stress.In all cases (Fig. 5 and S2 †), the storage modulus (G′) was higher than the loss modulus (G″) and no crossover is observed over the whole frequency range, showing a typical gel behaviour with a good tolerance performance against external forces.

Single crystal X-ray diffraction studies
Crystals of the three galactose derivatives (1, 2 and 3) were subjected to single crystal X-ray diffraction.The experimental data and structure refinement parameters are given in Table S1 (ESI †).Critical analysis of the crystal structures reveals that the pyranose ring remains in the typical 4 C 1 conformation for compounds 1 and 2. However, it has taken a skew boat conformation in the case of compound 3 which is a significant and interesting deviation from our observed results with the p-methoxyphenyl galactosides having similar functionalities reported earlier. 10ompound 1 crystallized in the monoclinic P2 1 space group with 2 molecules (Z = 2) in the unit cell (Fig. 6a).The p-thiotolyl ring of the molecule in the asymmetric unit and the sugar moiety are not co-planar.The torsional angles of the p-thiotolyl ring and the sugar moieties are 158.5Ĳ2)°(C5-S1-C8-C9)and −82.7Ĳ3)°(C8-S1-C5-C4), respectively, indicating that there is no planarity in the molecule.The four free hydroxyl groups in the molecule led to the formation of multiple intermolecular hydrogen bonds (selected bond lengths and angles are given in Table S2, ESI †).The sugar moieties of adjacent molecules are connected via multiple O-H⋯O hydrogen bonding interactions in the crystal structure and extended in 2D HBN as shown in Fig. 6b.As a result, the molecules form thick two-sided comb-like sheets running along the c-axis.In addition, the adjacent sheets are interlocked by the close packing of p-thiotolyl groups in a spacefilling manner and therefore form a 1D layer structure with intra-layer π-stacking interactions (Fig. 6b).
Compound 2 crystallizes in the monoclinic P2 1 space group with two molecules of compound 2 and one water This is © The Royal Society of Chemistry 2016 molecule in the asymmetric unit (Fig. 7a).The molecule contains two free hydroxyl functional groups along with a water of crystallization which form several O-H⋯O hydrogen bonds (Table S2, ESI †) and leads to the formation of a 2D HBN in the single crystal structure (Fig. 7b).The molecules form thick two-sided comb-like sheets interlocked by the close packing of p-thiotolyl groups of the adjacent sheets in a space-filling manner.As a result, the 2D HBN between water molecules and sugar molecules leads to a ladder motif along the b-axis (Fig. 7c).These layers are stacked along the b-axis to further stabilize the structure by multiple weak C-H⋯O interactions (selected bond lengths and angles are given in Table S2, ESI †).
Compound 3 crystallizes in the orthorhombic P2 1 2 1 2 1 space group with four molecules in the unit cell (Z = 4) (Fig. 8a).The p-thiotolyl ring of the molecule in the   asymmetric unit is not co-planar with a galactoside moiety.The torsional angles of the p-thiotolyl ring and the sugar moieties are 94.2Ĳ3)°(C5-S1-C8-C9) and 156.5Ĳ3)°(C8-S1-C5-C4), respectively, indicating that there is no planarity in the molecule.The molecule contains only one free hydroxyl group that forms a O-H⋯O hydrogen bond (O2-H2⋯O1; 2.858(3) Å, 165°) with the O-atom of the next pyranose ring.This leads to the conformational perturbation in the molecule by dragging the C9 of the sugar ring containing the free hydroxyl group downwards and the ring oxygen (O1) of the adjacent molecule upwards.In addition to the strong O-H⋯O HBN, an intramolecular C-H⋯O hydrogen bond (C15-H15⋯O2; 2.937(4) Å, 110°) is responsible for dragging C15 downwards.On the other hand, the isopropylidene ring gives the rigidity at C10 and C11 carbons and due to the required cis-geometry of the isopropylidene ring oxygen atoms, C10 and C11 remain in the same plane.The cumulative effects of these interactions bring O1, C8, C10 and C11 upward and they remain in a plane, whereas C9 and C15 go downwards resulting in a skew boat conformation of the pyranose ring (Fig. 8b).As far as our knowledge is concerned, this is the first report of such a conformational change in a sugar derivative driven by a hydrogen bonding network.
In addition to the interactions that seem relevant for the conformational change, there are other significant C-H⋯O hydrogen bonds that are present (Table S2, ESI †) in the crystal structure that led to a wave-type packing along the given axis (Fig. 9).

Powder X-ray diffraction (PXRD)
The PXRD patterns of compound 1 in the xerogel state was compared with the patterns obtained from samples in bulk state and the simulated PXRD pattern extracted from single crystal X-ray.The patterns are in good agreement with each other confirming that the molecular packing in different states is very much the same (Fig. 10).Normally, it is difficult to have clear PXRD patterns from gel samples due to their poor diffraction.However, the correlation between the PXRD patterns in different states, 16 is a useful tool to get an insight into the molecular packing.It is worth noting that xerogel samples can only be regarded as logical representative as the presence of other morphs or solvates cannot be ruled out.

Significant deviation from the reported p-methoxyphenyl galactosides with same substituent
Correlation with our previously reported series of galactose derivatives 10 revealed that there are significant differences in the supramolecular architecture of the thioglycoside derivatives reported here with that of the p-methoxyphenyl galactosides.Since the protecting groups present in both series are This journal is © The Royal Society Chemistry the same except the reducing glycoside, we expected to see a close similarity in their supramolecular behaviour.Indeed, compound 1 gave a strong gel and all compounds (1-3) gave quality crystals.Compound 1 and 2 have shown a 2D HBN, whereas compound 3 have a 1D HBN as revealed by their respective crystal packing.Therefore, in this series too, compounds 1 and 3 violate Shinkai's hypothesis 17 and the views reflected in the review by Dastidar 18 on the gelling and non-gelling behaviour and HBN.Compound 2 behaves in line with the hypothesis.Although the p-methoxyphenyl galactoside and p-thiotolyl glycoside series matched nicely, in-depth evaluation of the HBN showed significant difference between the two series.It is that an apparently minor difference in functionality contributes distinctly to the supramolecular arrangement in the crystal structures.In fact, the presence of sulphur at the reducing end glycoside plays a crucial role and influences the HBN significantly.Particularly in the case of the derivative 3, it results in the change of the regular chair conformation of the galactose unit to a skew boat conformation.

Conclusions
In summary, we have analyzed the role of HBN in the single crystal X-ray structures and gelation abilities of three p-thiotolyl galactosides and compared the data with the previously reported series of p-methoxyphenyl galactosides having similar protecting groups.There are similarities between the two series of derivatives with respect to their gelation and crystallization abilities.However, striking differences have been observed between the series considering the HBN involved.The presence of sulphur in the reducing end glycoside influences the difference in the HBN considerably and most interestingly, the altered HBN results in a change in the conformation of the galactose moiety from a regular chair form to a skew boat form in the case of compound 3 as evident from its single crystal X-ray structure.It is important to note that a minimal change in the protecting group decoration on the sugar molecule can have such an impact on the HBN in their molecular packing.

Fig. 6
Fig. 6 (a) ORTEP representation of the crystal of compound 1 with thermal ellipsoids drawn at the 50% probability level.(b) Molecular packing showing a 2D HBN in the crystal structure.Notice the formation of double-sided comb-like sheets via strong intermolecular O-H⋯O hydrogen bonds, interlocked by p-thiotolyl groups.

Fig. 7
Fig. 7 (a) ORTEP representation of the crystal of compound 2 with thermal ellipsoids drawn at the 50% probability level.(b) Molecular packing showing a 2D HBN involving sugar-sugar and sugar-water interactions in the crystal structure (except the hydroxyl H, all other H have been omitted for clarity).(c) The ladder motif along the b-axis.Notice the formation of double-sided comb-like sheets via strong intermolecular O-H⋯O hydrogen bonds, interlocked by p-thiotolyl groups.

Fig. 8
Fig. 8 (a) ORTEP representation of the crystal of compound 3 with thermal ellipsoids drawn at the 50% probability level.(b) HBN involved for the change of conformation in the pyranose ring (except the hydroxyl H, all other H have been omitted for clarity).Two discrete molecules are shown in a ball and stick model to illustrate the HBN responsible for conformational change.