Issue 75, 2016

A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

Abstract

Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with ∼20-fold increased activity in E. coli.

Graphical abstract: A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

Supplementary files

Article information

Article type
Communication
Submitted
21 Jul 2016
Accepted
19 Aug 2016
First published
19 Aug 2016

Chem. Commun., 2016,52, 11239-11242

A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

X. Zhu, P. Shieh, M. Su, C. R. Bertozzi and W. Zhang, Chem. Commun., 2016, 52, 11239 DOI: 10.1039/C6CC05990B

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