Selecting reactions and reactants using a switchable rotaxane organocatalyst with two different active sites† †Electronic supplementary information (ESI) available: Synthetic procedures and characterisation data. See DOI: 10.1039/c4sc03279a Click here for additional data file.

The activation mode of a rotaxane-based organocatalyst with both secondary amine and squaramide catalytic units can be switched with acid or base, affording different products from a mixture of three building blocks.


Introduction
Synthetic catalysts have previously been developed where a stimulus can be used to turn the catalytic activity 'on' or 'off' 1,2 or to change the stereochemical outcome of a reaction. 3 Here we report on an articial system that can switch between two different modes of organocatalysis, 4 each promoting a different chemical transformation. The result is a molecular catalyst that can be used to produce different reaction outcomes from a mixture of building blocks (Fig. 1).
The switchable catalyst employed is a [2]rotaxane in which the position of the macrocycle can be changed 5 to block one or other of two organocatalytic active sites. 2,6 The rotaxane (1/1-H + $CF 3 CO 2 À , Fig. 2) features a thread bearing dibenzylamine/dibenzylammonium and squaramide units as the catalytic centres. The activities of the organocatalytic sites are based on different activation mechanisms: the secondary amine/ ammonium unit is able 2 to promote iminium 7 (and potentially enamine 8 and trienamine 9 ) catalysis while squaramide-catalyzed reactions proceed through the activation of electrophiles by hydrogen bonding. 10 The macrocycle of the rotaxane contains a pyridyl-2,6-dicarboxyamide unit that can bind effectively to the squaramide residue, and a crown ether-like region that has a very high affinity for secondary ammonium ions but not for non-protonated amines. 11 A rigid spacer was introduced between the two active sites on the thread to prevent folding. Accordingly, when the rotaxane is protonated (1-H + $CF 3 CO 2 À ) Fig. 1 Different products from a mixture of building blocks using a rotaxane catalyst switchable between two different active sites (e.g. Fig. 2). Alternative reactions are promoted (involving particular functional groups on different building blocks) according to which active site of the catalyst is revealed (e.g. Fig. 4).

Fig. 2
Acid-base control of the position of the macrocycle in rotaxane 1 (iminium catalysis 'on'; hydrogen bond catalysis 'off')/1-H + $CF 3 CO 2 À (iminium catalysis 'off'; hydrogen bond catalysis 'on') and the structure of the corresponding thread 2-H + $CF 3 CO 2 À (both iminium catalysis and hydrogen bond catalysis 'on'). the macrocycle should preferentially encapsulate the dibenzylammonium group, masking it from being available for catalysis (iminium catalysis 'off') while leaving the squaramide site accessible (hydrogen bond catalysis 'on'). In the neutral form of the rotaxane (1) the squaramide should be the preferred binding site for the macrocycle, concealing it and making it unavailable for catalysis (hydrogen bond catalysis 'off') whilst leaving the secondary amine exposed (iminium catalysis 'on'). 12

Results and discussion
The synthesis of 1 utilized the intended pyridinedicarboxamidesquaramide recognition motif to promote the threading of a suitable squaramide derivative, 3, through the cavity of macrocycle 5, covalently capturing the interlocked structure through amide bond formation with bulky 'stopper' 4 ( Fig. 3, see ESI † for details). [2]Rotaxane 1-Boc was isolated in 47% yield along with the non-interlocked thread 46%).
The 1 H NMR spectra ( Fig. 3a-c) of the macrocycle (5), thread (2-Boc) and rotaxane (1-Boc) conrms the threaded architecture of 1-Boc with the macrocycle residing around the squaramide unit. The downeld shi of the H C amide protons in the rotaxane compared to the parent macrocycle (DdH C ¼ 0.19 ppm) and the shis of the protons on the central region of the polyether chain (DdH H ¼ 0.08 ppm; DdH I,J ¼ 0.25 ppm) indicate hydrogen bonding between the macrocycle and both sides, hydrogen bond donors and acceptors, of the thread squaramide unit. Protons of the phenyl rings of the macrocycle are shied upeld in the rotaxane (DdH E ¼ À0.26 ppm; DdH F ¼ À0.48 ppm) due to shielding by the ring currents of the squaramide ring and aryl substituents.
Deprotection of the dibenzylamine moiety using triuoroacetic acid afforded rotaxane 1-H + $CF 3    We investigated the ability of the rotaxane and the thread to perform organocatalytic reactions in both their protonated (1-H + $CF 3 CO 2 À ; 2-H + $CF 3 CO 2 À ) and unprotonated (1) states. Secondary amines can promote the Michael addition of 1,3dicarbonyl nucleophiles to a,b-unsaturated aldehydes via iminium catalysis. 13 When using a nitroalkene instead of the unsaturated aldehyde a similar Michael addition can occur if the electrophile is activated by hydrogen bond catalysts such as (thio)urea or squaramide derivatives. 14 Accordingly, we reasoned that the rotaxane might be able to catalyse the Michael addition of 1,3-diphenylpropane-1,3-dione (6) selectively to either crotonaldehyde (7) or trans-b-nitrostyrene (8) according to which type of organocatalytic group was exposed on the thread.
A mixture of 6 (0.5 M), 7 and 8 in a 1 : 2 : 1 ratio, 10 mol% NaOAc 15 and 5 mol% of the potential catalyst (1, 1-H + $CF 3 CO 2 À or 2-H + $CF 3 CO 2 À ) was stirred in CH 2 Cl 2 at room temperature (Fig. 4, top). Rotaxane 1 (secondary amine exposed) catalyzed the Michael addition of 6 to crotonaldehyde (7) to give 10 (40% conversion aer 72 h) with high selectivity (only a trace of 9, the addition product to trans-b-nitrostyrene, present in the reaction mixture as evidenced by 1 H NMR spectroscopy, Fig. 4c). Use of the protonated form of the rotaxane, 1-H + $CF 3 CO 2 À , (squaramide exposed) resulted in the formation of 9 with a conversion of 75% aer 18 h with only a few percent of 10 present in the reaction mixture (Fig. 4d).
In contrast to the selectivity found with both forms of the rotaxane catalyst, when the thread 2-H + $CF 3 CO 2 À was employed as the catalyst (both organocatalytic sites exposed) 9 and 10 were formed in a close-to-1 : 1 ratio (15% conversion aer 72 h, Fig. 4e).

Conclusions
A rotaxane with two different organocatalytic sites, a squaramide unit and a dibenzylamine group, separated by a rigid spacer, has been demonstrated to promote Michael addition reactions through either iminium ion or hydrogen-bond-activated catalysis. The system can be switched between the two activation modes through acid-base-mediated control of the position of the rotaxane macrocycle to conceal one site on the thread and reveal the other. The switchable organocatalyst was used to promote the Michael addition of 1,3-diphenylpropan-1,3-dione (6) to either crotonaldehyde (7) or trans-b-nitrostyrene (8) according to the catalyst state, with modest conversions (40-75%) and good selectivity in both modes. The ability to select which components of a mixture react together, affording different product outcomes from a common set of building blocks, is a promising use of articial molecular machines in chemical synthesis. 16