Issue 6, 2015

Silver nanoparticle toxicity and association with the alga Euglena gracilis

Abstract

The impact of silver nanoparticles (AgNPs) on aquatic algae has largely been studied with model species that possess a rigid cell wall. Here, we explored the interactions of AgNPs with Euglena gracilis, a green alga having no cell wall but a pellicle. The toxicity and silver uptake upon 1–2 h of exposure to various concentrations of AgNO3 and AgNPs, having a mean size of 47 nm measured in the exposure medium, were examined. The photosynthetic yield decreased in a concentration-dependent manner and AgNPs were less toxic than AgNO3 based on the total silver added. The cell morphology was significantly altered by AgNPs and AgNO3. The damaging effects of AgNPs on the photosynthesis and morphology were completely prevented by cysteine, suggesting that the toxicity of AgNPs was mediated by dissolved Ag. Indeed, the maximal quantity of cell-associated silver was higher upon exposure to AgNPs compared to that upon AgNO3 exposure, amounting to 5.1 × 10−4 mol Lcell−1 and 1.4 × 10−4 mol Lcell−1 for AgNPs and AgNO3, respectively. However, the difference was not caused by the cellular uptake of AgNPs, but by the strong sorption of AgNPs onto the pellicle.

Graphical abstract: Silver nanoparticle toxicity and association with the alga Euglena gracilis

Supplementary files

Article information

Article type
Paper
Submitted
30 Apr 2015
Accepted
21 Sep 2015
First published
21 Sep 2015

Environ. Sci.: Nano, 2015,2, 594-602

Silver nanoparticle toxicity and association with the alga Euglena gracilis

X. Li, K. Schirmer, L. Bernard, L. Sigg, S. Pillai and R. Behra, Environ. Sci.: Nano, 2015, 2, 594 DOI: 10.1039/C5EN00093A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements