Chromatographic methods for the isolation, separation and characterisation of dissolved organic matter † 1531 –

This review presents an overview of the separation techniques applied to the complex challenge of dissolved organic matter characterisation. The review discusses methods for isolation of dissolved organic matter from natural waters, and the range of separation techniques used to further fractionate this complex material. The review covers both liquid and gas chromatographic techniques, in their various modes, and electrophoretic based approaches. For each, the challenges that the separation and fractionation of such an immensely complex sample poses is critically reviewed. This critical review paper has been produced to aid those working the  elds of marine science and environmental geoscience, and related areas investigating carbon cycles, sources and fate. The authors are aware of the importance of separation science to the molecular characterisation and understanding of this important and highly complex environmental system, yet no de  nitive review in the literature focused on this subject exists. This review compliments a recent review published by Minor et al. on the structural characterisation of DOM, with greater focus on spectroscopic analysis and characterisation. We believe that togetherthetworeviewscovertheessentialpairingof ‘ detection ’ and ‘ separation ’ andcollectively o ﬀ


Dissolved organic matter
In simplest terms, the organic matter held within the global water system can be classied as either dissolved or particulate matter. Present within all marine and freshwater sources, dissolved organic matter (DOM) constitutes one of the Earth's largest carbon reservoirs, comparable to atmospheric CO 2 (624 and 750 gT, respectively). 1 Indeed, atmospheric CO 2 is directly inuenced by these global DOM reservoirs, as CO 2 is itself both a primary source of DOM via the activity of phytoplankton, and a primary product of DOM mineralisation. As DOM is an important component within the global carbon cycle, long term changes in environmental conditions and global systems, for example increasing levels of atmospheric CO 2 , ocean acidication, and global warming, could potentially affect those complex processes responsible for DOM production and removal. [2][3][4][5][6][7][8][9][10][11] Freshwater aquatic systems can also affect the global carbon balance by transporting terrestrially derived organic matter from land to the sea. [12][13][14][15][16][17][18][19] The input of terrestrial DOM represents 2-3% of the total DOM pool, however this percentage can increase when DOM from coastal areas is considered. 20 Up to 0.9 gT of carbon per year leaves the terrestrial environment and, of this, 0.25-0.7 gT is delivered from rivers to the sea, whereas 0.2 gT are from ground waters, discharging to the sea without entering rivers. 12,21 DOM is oen sub-classied as either labile (bioavailable) or refractory. The origins of refractory DOM have been the subject of debate for many decades, although primary sources of seawater or freshwater DOM, such as from soil, vegetation, oil seepages and wildres are well documented. 1,[22][23][24] More recently, the role of microbes in the conversion of labile DOM to the refractory form via the so-called 'microbial carbon pump' has been reported. 1 Microbial systems are able to metabolise and transform labile DOM from phytoplankton photosynthesis, viral lysis of bacteria and phytoplankton, and protozoan and zooplankton grazing. 1,[25][26][27][28] The bulk of the refractory moieties produced via this process persist within the water column, potentially for periods of several thousand years, without further transformation or digestion. 1 Key to a greater understanding of the complex system of biogeochemical processes involved in the formation and removal of DOM is an understanding of the exact nature of DOM itself. Investigations into the content and nature of extracted DOM date back over a century, and research effort in this area increases annually ( Fig. 1 shows the research papers published annually based upon an article title search (Scopus) using the term 'dissolved organic matter'). Traditional denitions of what constitutes DOM, of which most are based on ltration, are now being challenged through increasingly powerful (in terms of resolution) molecular studies. Such studies have pointed to what is more accurately described as "an organic matter continuum", 1,29 with materials ranging in size from the diverse mass of small organic molecules (<1 kDa), to organic colloids, to sub-micron particles, to large and structurally diverse natural polymers. Indeed, the complexity of DOM is such that no reliable estimates of the number of classes of compounds present are available, let alone rm ideas on the number of individual compounds. A further source of complexity, in terms of molecular resolution (physical separation) of this immensely complex material, is the issue of concentration, with compounds present within the range of micromolar to sub-picomolar levels. 30 Compounds present within DOM can also be classied according to polarity, which ranges from high to very low. Within this polarity spectrum, the following functional groups can be found in abundance: substituted alkyl carbons, unsaturated carbons, amides, carboxylic groups, aldehydes and Dr Sara Sandron received her Ph.D in Analytical Chemistry, from Dublin City University (Ireland). Her work focused on the application of multi-dimensional and multimodal chromatography to the separation of Dissolved Organic Matter (DOM). At present, she is undergoing a postdoctoral fellowship at the Australian Centre for Research on Separation Science (ACROSS) at the University of Tasmania, Hobart, Australia. The project also focuses on the use of novel multidimensional separation approaches to resolve DOM. Her current research interests include sample preparation techniques, liquid chromatography, gas chromatography, high-performance counter current chromatography, mass spectrometry and nuclear magnetic resonance. utilised NMR to characterise seawater DOM, reporting the following prominent features: aliphatic C-H and C-C bonds, C-N carbon linkages, aliphatic C-O linkages typical of alcohols, esters, ethers and anomeric carbons, aromatic and olenic carbon linkages, carbonyl groups of amides, carboxylic acids, esters and ketones, with less signicant phenol peaks coming from tannin and lignin-like materials. 32 Flavonoids and simple phenolics add to this complex mix. These classications are commonly supported with data obtained from high resolution mass spectrometry (HR-MS). 22,[32][33][34][35][36][37][38][39][40][41][42][43][44][45][46] The above functional groups are found within major classes of compounds, such as amino acids, proteins, peptides, sugars, amino-sugars, carboxylic rich alicyclic molecules (CRAM), materials derived from linear terpenoids (MDLT), neutral lipids, DNA, RNA, and sterols. [30][31][32][33][47][48][49][50] Despite the wealth of literature on the nature and classes of compounds present within DOM, there remains a great deal to be revealed regarding its exact composition, how such complex material and chemical systems interact, and how composition varies between seawater and freshwater, geographically and seasonally. Two analytical approaches are used for the chemical characterisation of DOM, methods either based upon the direct analysis within the water sample itself (e.g. bulk measurement, such as uorescence or nuclear magnetic resonance spectroscopy (NMR) 51 ), or upon the analysis of extracted DOM. 30,31 The former potentially avoids contamination and artefacts, but is generally low resolution and not suited to the identication of organic compounds at nano-or picomolar level, particularly when present in saline samples. 31,[51][52][53] The latter approach is restricted by the limited availability of well-characterised extraction techniques available for DOM isolation.
Even with a 'standard method' for obtaining DOM (for which there is currently none), such diversity in structure, size and concentration would present a considerable analytical challenge, with the need for ultra-high resolution analytical technology to mine such samples for molecular denition. Such advanced instrumental approaches to DOM, predominantly mass spectrometry (MS) and NMR based methods, were reviewed in 2007 by Mopper et al., together with discussion on DOM extraction techniques applied to marine samples. 31 Later, in 2011, both Hutta et al., and Duarte et al., critically outlined the most prominent methods to analyse, fractions of DOM, such as humic substances and water soluble organic matter from atmospheric aerosols. 54,55 Within both cases, the importance of chromatographic methods prior to advanced detection and identication methods was strongly emphasised, but not reviewed in detail. A more recent review by Minor et al., focussed on the structural characterisation of DOM, approaches to DOM extraction, and bulk characterisation using spectrophotometry, MS and HR-MS, NMR and Fourier transforminfrared spectroscopy (FT-IR). 56 Mostly absent in each of the above excellent review papers, is a detailed analysis of signicant role separation science has played, and continues to play, in the molecular characterisation of DOM. This aspect of the published literature on DOM characterisation has yet to be the subject of a dedicated review and is certainly worthy of critical discussion. This review therefore selectively covers DOM extraction, fractionation and highperformance separation methods, including both liquid and gas phase chromatography and highlights aspects where advances in separation sciences have had, and will have, a major impact in helping to resolve such complex organic mixtures.

Isolation of dissolved organic matter
Scheme 1 shows the range of separation methods used in the isolation and separation of DOM, in what oen involves 3, 4 or 5 separate procedures/dimensions. In each step the critical role selectivity plays in any nal analytical characterisation is very clear. Table 1 includes each of these methodologies and summarises the main purpose of the process, the inherent selectivity (or lack of) and examples of particular applications. Sampling and isolation of DOM represents the rst step in all published analytical studies, and it is this rst step which possibly presents the biggest challenge in understanding the Prof. Brett Paull is a B.Sc, Ph.D and D.Sc graduate University of Plymouth, and an RSC Fellow. His rst lectureship was at the University of Tasmania (1995 to 1997), before moving to Dublin City University (1998)(1999)(2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011). In 2011 Brett rejoined the University of Tasmania as Professor, and is currently Director of the Australian Centre for Research on Separation Science (ACROSS). Brett's research interests lie in the elds of analytical/bioanalytical chemistry, and materials science. His work is documented in over 200 publications, including 170 peer review journal articles. Within ACROSS research focusses upon production and characterisation of new materials and platforms for application within the analytical/bioanalytical sciences, and advanced inorganic and organic materials for selective extraction and separation purposes. exact composition of DOM, namely achieving efficient, reproducible extraction of representative, uncontaminated samples, with acceptable recoveries. The rst stage of this process involves initial sample ltration to remove particulate matter. This ltering steps applied dene DOM according to the porosity of the lter itself. Initial work in this area in the 1970s, applied glass bre lters (GF-F) lters with pore size ranging from 0.45 to 1.0 mm for the isolation of DOM. 30 Nowadays, the lters used to separate POM from DOM have pore size ranging from 0.2 to 0.1 mm. According to this size-based fractionation, POM commonly includes pollen and small organisms such as zooplankton, phytoplankton and bacteria, whereas DOM comprises classes of compounds such as viruses, macromolecules and small molecules (1000-0.1 nm). 30 Filters applied to DOM isolation have been traditionally heat treated (calcined) to remove organic contamination, and solvent washed prior to use. 30,31,56,57 However, clearly given the idea of the "organic matter continuum", the current denition of DOM on the basis of lter porosity is an imperfect one. All the compounds considered to constitute DOM pass through these lters, while those classied as particulate organic matter (POM) do not.
Second to exactly what is being extracted, is how much can be extracted, given the need to obtain sufficient sample for subsequent analysis. This itself is challenging when considering the volume required (oen obtaining, handling and storing 25 to > 100 L) and varying degrees of sample salinity, which in the case of seawater contains 20-35 g L À1 of inorganic salts, compared to 1-3 mg L À1 of DOM (thus selective desalting is of primary importance, particularly prior to MS analysis). 30 Preservation of collected water samples prior to DOM extraction should minimise loss of sample integrity, which is not trivial, given the chemical heterogeneity of DOM. For example, the acidication of a water sample to pH 2 can degrade the sample, denature proteins and peptides, and change the reactivity of some classes of compounds within DOM. However, it is very difficult to understand any such changes that DOM may undergo aer extraction and practically impossible to compare the chemical characteristics of the original liquid sample with those of the solid/reconstituted material recovered aer isolation. Further, any precise evaluation of extraction procedures is hampered by a lack of reference materials. As the composition of DOM is dependent upon the sampling location and season, it is not possible to obtain a universal reference DOM standard. 31 Reproducibility studies on DOM samples obtained from analogous locations and times are also unavailable, which further underlines our lack of knowledge regarding inter-sample  59, 64, 76, 107, 132, 189, 190, 193, 197-201, 204-206, 208-212, 214 variability. From an operational point of view, one is unlikely to obtain identical samples from the same location at different time-points, as currents, seasonal variability and weather conditions affect sample reproducibility.
However, despite the above challenges, several widelyaccepted protocols for DOM extraction have been developed, some now viewed as pseudo-standard methods. In addition, the International Humic Substances Society (IHSS) now provides reference materials, which are commonly utilised as standards for method development and validation. 58 The most widely used reference standard is Suwannee River DOM, with organic carbon concentrations from 25-75 mg L À1 and pH of approximately 4.0. However, the IHSS does not guarantee that successive collected batches are fully identical, and given its freshwater nature, Suwannee River material is not ideally representative of seawater DOM.
Ultraltration (UF) and solid-phase extraction (SPE) are the most commonly used techniques applied for DOM extraction (see Tables 2 and 3), and are in detail discussed separately below. The two approaches differ signicantly, not least as UF is a physical process (based on mass discrimination), whilst SPE is based on the solute partition coefficient between sorbent and aqueous phases, and hence greatly dependent upon solute and phase chemistries. Unsurprisingly, the fundamental differences between these techniques can produce several compositional differences within the extracted DOM. 31,57,[59][60][61][62][63] For both UF and SPE, it would appear that recoveries for marine DOM can be highly variable, and thus it is questionable if the extracted DOM can be regarded as being truly representative. 31 In addition, when applying these extraction procedures, retentates are oen freeze-dried to facilitate sample storage, 64 which for labile materials within DOM (i.e. proteins) presents the additional risk of structural damage from ice crystal growth if the rate of freezing is too fast and large crystals are formed. Further limitations of these methods include, contamination due to bleeding/leaching of polymeric material (e.g. from polymer resins or membranes), side reactions with DOM functional groups and the irreversible adsorption of DOM components from the solid support, particularly in the case of SPE. [65][66][67][68][69] Due to the large volumes of water that are commonly extracted, SPE is usually used in off-line modes, however this procedure is time consuming and oen requires many steps before obtaining a sufficiently concentrated sample, increasing the risk of contamination, sample loss and degradation.
Combined techniques for DOM isolation and desalting DOM, 70-74 such as reverse osmosis (RO) and electrodialysis, can improve sample recovery (up to 95%), but are currently less commonly applied to DOM isolation than UF or SPE, likely due to the relative availability of the technique, but maybe also related to higher costs involved, and the need for more rigorous blank conrmations. 61,62 For example, within recent studies, RO coupled to electrodialysis was found to be at least twice as expensive as SPE. 62 In addition, there are also some reports that indicate the DOM extract obtained from reverse osmosis coupled to electrodialysis can contain high levels of inorganic matter. 62,75 Clearly the above studies and observations point to some clear advantages and potential disadvantages of each approach (e.g. ease of use and cost of SPE, but with variable and limited recovery, compared to the availability and cost of RO, but which can provide excellent recovery). From the literature published there is certainly no obvious consensus as to the best approach to apply at this time, although it is clear that data generated from subsequent analysis and characterisation should be viewed with regard to the approach used and the inherent limitations thereof. The following sections present the applications of each extraction and isolation technique in individual detail.
1.2.1. Ultraltration. Ultraltration systems, as used in industry, exist in several congurations, such as cross-ow or tangential ow ultraltration and stirred cell ultraltration. Benner et al. were amongst the rst to use UF to extract DOM in 1992, and this approach has since been modied by various research groups (Table 2), to enhance recovery and integrity of the sample. 30,52,56,[76][77][78][79] A nominal molecular weight (MW) cut-off of 1 kDa is typically used for DOM isolation by UF (Table 2). When extracting freshwater DOM, the recovery by UF is oen higher than SPE, and does not normally require any chemical pretreatment of the sample (Tables 2 and 3). This improved recovery has been attributed to the higher average MW of freshwater material compared to seawater DOM. Using UF with seawater derived samples also sees retentate solutions rich in inorganic salts, and therefore further desalting procedures are commonly needed. 36,37 To address this Abdulla et al., applied dialtration with deionised water following the concentration step. 80 UF typically involves higher sample ow rates, together with large surface area polysulfone or polyamide membranes, giving the possibility to extract large sample volumes relatively quickly, a considerable benet of the technique (Table 2 and ESI Table  S1 †). However, MW fractions with sizes lower than the membrane cut-off are not retained onto the membrane, and membrane contamination and adsorption issues are occasionally encountered. Additionally, there is a need to carefully optimise operating parameters, and membrane conditioning procedures. 30,31,56 Considerable variability in membrane performance and systems from different manufacturers has been observed, as well as between laboratories using the same UF systems. 52,56,81,82 When UF is not combined with other extraction techniques, such as SPE, reported DOM recoveries have ranged from as low as 8% to 55% for marine samples, and up to 80% for freshwater DOM (Table 2). 76,82,83 However, UF yield is reported to be tightly dependent upon salinity levels. 56 Lower extraction efficiency is attributed to lower occulation at higher salinity. 56,77,78,84,85 It has also been observed that DOM recovery is also somewhat depth-dependent. Lower recoveries have been observed for deep water samples when compared to surface water equivalents. 56 Table 2 Overview of the membrane-filtration methods for the isolation of DOM (membrane details in ESI Membrane details such as material, pore size and molecular cut-off, which is indicative of the size range of the extracted sample, ODS: octadecyl silica, MMA: methyl methacrylate copolymer, PS-DVB: polystyrene divinylbenzene. c DT-MS: direct temperature-mass spectrometry, NDIR: non-dispersive infrared, other abbreviations as in Scheme 1.

Table 3
Overview of the SPE methods for the isolation of DOM (SPE substrate details in ESI Table S2)  hydrophobic polymeric resins (e.g. XAD™), alkyl-and arylsilicas (e.g. C 18 -functionalised silica, Table 3) and ionexchangers. In the majority of cases these SPE sorbents display predominantly hydrophobic properties and are commonly preactivated/conditioned using polar organic solvents such as methanol (MeOH) or acetonitrile (MeCN). Pre-ltered seawater or freshwater samples are generally rst acidied before extraction to improve the recovery of carboxylic-and phenolicrich species, which exhibit maximum sorption onto such sorbents at pH < 4. The adsorbed DOM is then commonly eluted using MeOH or MeCN, as is standard SPE procedure with arylsilicas and hydrophobic polymeric resins. As mentioned above, potential problems associated with SPE include the contamination of isolated DOM, resulting from the leaching of material from the sorbent, (although this can be minimised through appropriate conditioning and wash procedures), together with any impact upon DOM arising from sample acidication, as it is not clear to what extent such treatments modify molecular structures and composition. 30,78,[88][89][90] Clearly, when using an SPE based extraction procedure, only those classes of compounds with affinity towards the selected sorbent will be isolated, which may translate to signicantly lower recoveries compared to UF (Tables 2 and 3). Unless multiple SPE cartridges with complementary chemistry are used (e.g. combination of polar and apolar phases), it is difficult to extract the complete spectrum of compounds present in DOM. This presents a substantial hurdle to overcome when attempting to fully characterise this complex material. Despite these issues, SPE, particularly where automated (which is readily achievable), still represents perhaps the most practical option for DOM extraction, particularly in sample processing times and costs. 62 SPE also provides the opportunity to introduce desired selectivity into the extraction procedure for more targeted studies. Together these advantages typically outweigh the above limitations and SPE remains a popular approach to DOM extraction, as demonstrated by the following methods and applications.
Non-ionic macroporous polymeric sorbents (e.g. XAD™) are typically formed from hydrophobic copolymers, displaying different extraction selectivity and capacity, reecting their specic chemical and physical properties (i.e. surface area, porosity, % cross-linking etc.). The range of XAD resins reported within the literature for DOM isolation include XAD-2, XAD-4 and XAD-8 (Table 3). XAD-2 and XAD-4 have analogous chemical structure, both being poly(styrene-divinylbenzene) resins (PS-DVB), but with differing surface areas (330 m 2 g À1 and 725 m 2 g À1 , respectively). 91 XAD-8 has a similar surface area to XAD-2, but surface chemistry that is based upon a cross-linked poly-(methylmetacrylate) (ESI Table S2 † provides specic details on the physical and chemical nature of these and other sorbents used for the extraction of DOM).
The above XAD resins have been widely used in the past to extract DOM from natural waters and are reported to provide acceptable recoveries, together with the capacity to process large volumes of water (Table 3). 31,59,67,79,[92][93][94][95] When compared to material extracted using UF or alternative SPE sorbent, DOM obtained using XAD resins tends to show the lowest H/C ratios, and is characterised by a higher proportion of condensed aromatic moieties, typical of avonoids and lignin-like materials. 61 Extracted material is also reported to be relatively low in aliphatic and lipid-like moieties, which might lead to an underestimation of the hydrophobic portion of DOM. 93 The use of XAD in DOM extraction involves thorough washing sequences with both organic solvents and aqueous solutions prior to use to reduce extensive sample contamination, 30 oen requiring multiple elution steps (considered a harsh extraction process). The exact retention mechanism exhibited by XAD resins has been discussed by Town et al., who proposed the potential for additional size-exclusion interactions, 67 whilst other studies highlighted the existence of p-p interactions between aromatic compounds (i.e. lignin-like materials and humic substances) and aromatic structures on the resin surface. 96,97 Alternative extraction phases to XAD resins are now more commonly used in DOM isolation, details of which are discussed below. However, as mentioned within Green et al., XAD resins still represent the most economically attractive technique in terms of equipment and extraction costs. 62 Several alternative PS-DVB adsorbents for DOM recovery have been investigated by Roubeuf et al., (SUPELCLEAN ENVI-Chrom P) and more recently by Dittmar et al., (PPL BOND ELUT), with the latter sorbent described as a PS-DVB phase modied with a proprietary non-polar surface (Table 3). 57,61,90 This particular sorbent exhibits a high surface area (600 m 2 g À1 ) and offers signicant retention of both non-polar and polar solutes, providing improved selectivity for the full range of compounds constituting the bulk of DOM (including CRAM). Such PS-DVB phases are also noted for their recovery of small molecules (<3 kDa), and recoveries of up to 62% have been reported (Table 3). 57 Following these studies, SPE methods employing the above PS-DVB-based resins have seen widespread acceptance.
Although still requiring sample acidication to maximise recoveries, the sample obtained from these new PS-DVB phases has been deemed to be acceptably representative of the true DOM composition 61 and according to Dittmar et al., the use of these PS-DVB-based resins allows for the isolation of molecules with polarity degrees ranging from highly polar to nonpolar. 57 However, NMR spectra of DOM extracted using SPE with new PS-DVB-based sorbents indicate the extract is predominantly low polarity material, the bulk of which include aromatic groups, indicative of terrigenous origin. Additionally, when compared to other extraction techniques, relatively high CRAM and nitrogen contents have been reported, the latter an indication of higher recovery of solutes containing amino or amide groups, such as protein derived materials. 61 Hydrophobic silica-based SPE sorbents are also applied in DOM extraction. The most widely used sorbents are well characterised C 18 -functionalised silica gels, typically applied to the extraction of non-polar to moderately polar compounds. The application of SPE using C 18 -functionalised silica sorbents dates back to the early 1980's, with studies such as those reported by Mills et al. (Table 3). 98 Several years later, the same group compared recoveries and selectivity of alternative functionalised silica sorbents, such as: C 2 -, C 18 -and phenylbonded silica. 99 In this work, cartridges were pre-rinsed with MeOH, 0.3 mM HCl, loaded with the sample, and eluted with MeOH and nally, deionised water. Relative composition of the isolated DOM samples was investigated using reversed-phase liquid chromatography (RP-LC). Phenyl-bonded silica gel was reported to show the highest recovery of the sorbents investigated (up to 27%), followed by C 18 and C 2 -functionalised silica. More recently, Dittmar et al., also compared the efficiency of a number of C 18 -functionalised silica sorbents with PS-DVB based resins, including a non-endcapped C 18 -silica based sorbent (C 18 OH), which was reported to extensively contaminate the sample due to bleeding. 57 Although highly variable, comparative studies such as that carried out by Dittmar et al., 57 have reported that C 18 -functionalised silica sorbents show similar, but slightly lower recoveries to those achievable using PS-DVB-based adsorbents, with NMR and HR-MS based characterisation suggesting that both types of sorbent generally extract analogous classes of compounds. 57,100,101 However, points of difference include DOM from C 18 -functionalised silica seeming to exhibit a lower nitrogen content and higher H/C ratio, the latter indicative of strong retention of aliphatic compounds (i.e. lipids and terpenoids) 61 or carbohydrates. PS-DVB resins exhibit a higher affinity towards compounds having aromatic and double or triple bonds.
PS-DVB, alkyl-and phenyl bonded silica are mainly designed for the extraction of hydrophobic and low polarity molecules. Ion-exchange based SPE extraction can be used for isolation of hydrophilic organic substances. Perminova et al., recently compared traditional DOM extraction methods and extraction based on the use of a diethylaminoethyl (DEAE) anion-exchange cellulose. 61 In this work freshwater samples were loaded onto the DEAE sorbent and eluted with 0.1 M NaOH. Recoveries of up to 82% for DOC were reported ($10-15% higher than the traditional approaches, namely C 18 -functionalised silica, PS-DVB and XAD-8™), however, the study found the DEAEextracted DOM to be enriched in highly oxidised structures, such as polyhydroxyphenols, organic acids and carbohydrates. 61 NMR data also showed a lower proportion of alkyl-chain protons and higher contributions from carbohydrate and aromatic protons, verifying that this DOM sample differs materially from DOM extracted using traditional sorbents. Based on these ndings, the authors suggest the extracted material does not correspond to typical DOM compositional prole seen from the majority of former studies, and conclude by recommending the use of the SPE technique from Dittmar et al., based on the PS-DVB sorbent. 57,61 Following on from the above comparative studies, Swenson et al., recently developed a novel SPE system based upon the use of two different kinds of extraction columns, which could be either applied coupled or in single mode. 102 A PS-DVB-based stationary phase was coupled to a second cartridge containing an activated carbon phase, providing recoveries which were found to be higher than those obtained when a single extraction chemistry was used. The cartridge eluate was loaded directly onto a RP-LC analytical column operating in gradient mode (water/acetonitrile 0.1 M formic acid) with MS and/or UV-Vis detection. 102 1.2.3. Miscellaneous batch extraction. The isolation of DOM from potable waters using anion exchange resins is well documented but includes the more recent development of magnetic ion exchange resins (MIEX). 103 Boyer et al., have extended this approach to a variety of environmental waters, using 'magnetically enhanced' macroporous poly(acrylate) strong anion-exchange resins. 104,105 Extractions of well characterised DOM isolates, which covered a range of molecular characteristics, such as carboxyl acidity, aromaticity, MW and nitrogen content, were carried out over four day periods to ensure equilibrium was reached and to construct anion exchange adsorption isotherms. The authors concluded charge density to be the key molecular property affecting DOM recovery using such MIEX resins, noting that microbially derived DOM, having low charge density and low aromaticity, exhibited the least affinity for the sorbent. The presence of a high salt content, particularly sulphates, were also noted to reduce DOM extraction efficiency.
The use of multi-walled carbon nanotubes (MWCNTs) in SPE has been explored in the isolation of pollutants from aquatic streams, and in 2007 also for DOM extraction (Table 3). 106 Su et al., studied the adsorption kinetics and thermodynamics of DOM onto this newly proposed material, achieving recoveries up to 95%. Prior to extraction, MWCNTs were thermally treated at 400 C to remove amorphous carbon and adsorption experiments were conducted using 30 mg of adsorbent in 200 mL of DOM solution (pH range ¼ 3 to 9). The solution was subsequently ltered to recover adsorbents, which were further reactivated through a N 2 gas ow. This procedure was repeated ten times in order to maximise DOM recovery. DOM was found to be negatively charged across the solution pH range investigated, with the negative charge increasing with pH due to ionisation of carboxylic groups, which were found to be a prominent functional group together with phenolic groups and hydroxyl groups. DOM adsorption and desorption rates were found to be temperature dependent, with higher adsorption at lower temperatures and, conversely, greater desorption at higher temperatures. More recently, Sánchez-González et al., modied this procedure and applied their method to the isolation of DOM from seawater. 107 In this case, 60 mg of sorbent were used for 250 mL of seawater, adjusted to pH 3. Desorption of DOM was carried out at pH 10, and the extract further characterised by means of size exclusion chromatography (SEC) (see Section 2.1.2). However, despite the reported high recoveries, the selectivity of MWCNTs for DOM as a whole still requires further clarication, particularly in comparison to previously discussed traditional SPE sorbents.
1.2.4. Solid phase microextraction. Solid phase microextraction (SPME) is a solvent-free non-exhaustive extraction method, commonly used for concentration of volatile compounds prior to thermal desorption and separation by gas chromatography (GC) (see Section 2.2). In this case the sorbent material is attached to the surface of a bre, and can be used for either liquid or gas phase extraction. 108 Properties of DOM have been explored using SPME, such as their affinity to bind other organic substances in solution. This includes phenols using polyacrylate coated SPME bres, and most recently polycyclic aromatic hydrocarbons (PAH) using polydimethylsiloxane-DVB coated bres. 109,110 Due to its microscale format, SPME is however only applicable to analysis of DOM, not for the preparative isolation.
1.2.5. Combined techniques: reverse osmosis/electrodialysis. Reverse osmosis (RO) is an extraction technique commonly used in industry and, above all, in water ltration and desalting. However, RO has also been widely used in DOM extraction, providing the possibility to treat large water volumes without sample acidication. [111][112][113] This technique was introduced in the 80s and applied by Perdue et al., and Clair et al., to the extraction of DOM from freshwater and surface waters. 111,[114][115][116] However, during isolation, RO can concentrate both organic and inorganic species, and therefore RO is generally applied in combination with electrodialysis or, less commonly, XAD™ resins, nanoltration systems, or cation exchange membranes (Table 2). 71,72,114,117 The extraction technique itself can involve relatively harsh chemical conditions to remove DOM from the RO membrane, with NaOH rinses (pH 12) potentially degrading certain DOM components, such as proteins and peptides.
In a series of publications from Koprivnjak, Vetter and coworkers, 70-72 the combination of RO and electrodialysis was reported to achieve enhanced recovery of DOM from both freshwater and seawater sources. The rst demonstration of this approach was in 2006, applied to processed synthetic river water samples and obtaining extraction yields of up to 92%. Later, Vetter et al., 71 applied this technique to real seawater samples, and reported a recovery of organic carbon of up to 90%. However, large amounts of inorganic salts were still contained within the extracted sample, signicantly higher than that existing in the extract reported by Dittmar et al. 57 Koprivnjak et al., 72 reported 75% extraction efficiency for seawater derived DOM from a similar location. In this case, DOM was analysed by both NMR and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS), which showed the sample to be comparable to the extracts obtained through UF. Koprivnjak et al., also compared their NMR spectra to those obtained by Hertkorn et al., 32 and underlined the presence of CRAM-like materials, together with differences in composition between non-coastal and coastal DOM (i.e. enrichment in terrestrially-derived molecules in the case of coastal DOM). Interestingly, within their review, Mopper et al., 31 suggested DOM extraction through the combination of RO and electrodiaysis is likely to provide a more representative material, and Koprivnjak et al., 72 did indeed observe additional peaks within their DOM NMR and FT-ICR-MS spectra, as compared to previously employed SPE based procedures.
More recently, in order to further conrm the more representative nature of DOM samples obtained through RO coupled with electrodialysis, Chen et al., analysed the isolated seawater DOM by means of ultrahigh resolution MS. 75 Samples from two different locations (Atlantic and Pacic Ocean), each at three different depths, showed a signicant number of common features (i.e. from 54 to 79% of the assigned molecular formulae), underlining inter-and intra-location analogies. The most signicant differences were found within surface samples, characterised by higher H/C values. The authors related these ndings to the degradation of aromatic compounds and the production of aliphatics and carbohydrates within surface waters. Furthermore, samples from the Pacic generally showed higher O/C values compared to those from the Atlantic, suggesting an enhanced degree of oxidation, which is possibly related to an enhanced microbial activity or remineralisation processes. The degree of intra-sample similarity suggests that a signicant fraction of the extracted DOM is refractory in nature and many of the molecular formulae from these refractory moieties were also found within previously analysed freshwater samples. 43,100,118 The study highlighted the representative nature of DOM obtained through RO with electrodialysis, a nding also conrmed upon calculation of the C/N ratio of the extracted samples, which was comparable to direct measurements obtained from the original seawater sample. 75 1.2.6. Passive sampling. In 2006, Lam and Simpson were the rst group to propose passive sampling as an alternative extraction method to isolate freshwater DOM (Table 2). 119 Passive samplers can be described as devices allowing the transfer of analytes from sampling media (i.e. seawater or freshwater) to a receiving phase, which can be a solvent or a porous sorbent. This extractive technique can operate in kinetic or equilibrium mode, therefore affecting sample isolation. Within kinetic passive sampling, the sample uptake to the receiving phase follows a rst order rate. The rate of mass transfer to the receiving phase is proportional to the difference between the chemical activity of the analyte in the sampling media and in the reference phase. Conversely, equilibrium sampling allows the establishment of a thermodynamic equilibrium between sampling media and receiving phase. For this reason, stable analyte concentrations are achieved aer a set time. 120,121 The apparatus used by Lam and Simpson 119 (see Fig. 2) consisted of an in-house constructed high-density polyethylene casing with pre-drilled holes containing a size-selective poly-(vinylidene uoride) (PVF) membrane and a DEAE functionalised exchange resin. The PVF membrane allowed the extraction of DOM with a MW lower than 1000 kDa, whereas the anion exchange resin was employed to concentrate negatively charged species (only suitable for freshwater systems). This extraction technique presents some clear advantages over UF and SPE procedures, such as the elimination of many potential sources of contamination arising from water sampling and associated sample handling/storage. There is also no need of sample pumping in passive systems and DOM can be concentrated from discrete depths at low cost. 122 An obvious practical disadvantage of this technique is however sampling time. For example, Lam and Simpson reported excellent recoveries of between 72 and 89% from 10 ppm DOM solutions under laboratory conditions, but this was carried out over an extraction period of two weeks. In eld experiments the authors deployed multiple samplers with a ratio of 250 mg of resin per 7 cm of membrane, over a similar two week period, enabling the isolation of an impressive 2.8 g of DOM.
In a more recent study, McCaul et al., utilised similar passive samplers to those described above, deployed over a four week period to isolate and study the composition of lacustrine freshwater DOM. 123 NMR spectra proved to be similar to those obtained from Lam et al., 119 showing the existence of representative classes of compounds such as: CRAM, MDLT, ligninlike materials, amino acids, proteins, peptides and carbohydrates. The same experiments also supported the presence of molecules typically derived from soil, plants and human activities (i.e. peptidoglycan, phenylalanine, lipoproteins and large polymeric carbohydrates).
In summary, although a promising approach, local conditions such as temperature, water movement, turbidity and biofouling could signicantly affect the efficiency and selectivity of passive sampling. To help overcome these issues, reference compounds should be used to reduce and quantify the impact of such environmental parameters. 124,125 2. Chromatography of dissolved organic matter Typically following above mentioned isolation procedures, which aim to isolate and concentrate DOM, high-performance chromatographic techniques are mainly applied in an attempt to fractionate and separate the extracted DOM into its many different classes of compounds. To do so, different chromatographic methods have been applied, once again exploiting differences in compound polarity, shape, size, charge, volatility etc. The need for this additional simplication/fractionation step is quite clear, as discussed within the 2007 review of Mopper et al., who note the limitations of many analytical techniques when applied to direct DOM characterisation. 31 Non-selective analytical methods only describe only bulk properties, or limited fractions of the total DOM pool, for example, total organic carbon (TOC) measurements, C : N ratios, or bulk uorescence. Such approaches reduce DOM to an average theoretical material, with a characteristic ngerprint, which is oen used for identication of the source, bulk transport and comparative studies of water bodies. 31,[126][127][128][129] For molecular level information, only MS and NMR (particularly HR-MS or multi-dimensional NMR) can begin to approach the level of selectivity required, 32,33,36,80,101,[130][131][132][133][134] although the complexity of the unfractionated material oen results in extensive spectral overlap. 135 Thus, the challenge currently sits in nding the right chromatographic approach to achieve DOM fractionation/separation prior to such HR-MS and NMR analyses.

Liquid chromatography
The following liquid chromatographic methods have all been applied to the fractionation and separation of DOM; RP-LC and normal phase liquid chromatography (NP-LC), SEC, hydrophilic interaction liquid chromatography (HILIC), ion exchange chromatography, silver ion chromatography, and most recently, high-performance counter-current chromatography (HPCCC). These various techniques have been applied in attempts to fractionate DOM into classes of compounds according to polarity (hydrophobicity/hydrophilicity), MW, charge, and degree of unsaturation (Tables 1 and 4). The following sections detail these approaches and applications thereof individually, followed by some summary and comparative observations.
2.1.1. Reversed-phase LC with UV and/or uorescence detection. Bulky complex organic structures common in DOM 32,33,134 oen exhibit strong retention in RP-LC, which necessitates relatively strong mobile phase gradients for elution. Additionally, the 'sticky' nature of such materials also demands frequent blank runs between samples to eliminate instrumental carryover and fully regenerate the column. However, despite these requirements, RP-LC remains a popular approach for DOM fractionation.
Mills and Quinn were amongst the very rst to use RP-LC (with UV detection) fractionation for DOM samples from an estuarine source in 1981. 98 A water/MeCN mobile phase gradient was used with a 300 Â 3.9 mm i.d. mBondapak C18 column. Although each chromatogram was dominated by several clusters of largely unresolved peaks, the largest of which eluted in the middle region of an applied MeCN gradient (suggesting intermediate polarity), each clearly showing specic features according to sampling location (see Fig. 3). Mills and co-workers later reported further application of this RP-LC method to estuarine DOM samples, following minor improvements, such as use of a buffered mobile phase (pH 3.2 with H 3 PO 4 ). 99 However, once again most of the detectable DOM components eluted within a similar gradient window as an unresolved 'hump', although large unretained peaks eluting at beginning of the chromatograms did indicate the presence of a signicant fraction of highly polar organic material.
Lignin-derived phenols are widely used to understand the transport of terrestrial organic matter and have also been analysed using RP-LC, on the basis of previously reported methods. 16,[136][137][138] Within one such study, terrestrially derived  Table 4 Overview of the LC methods applied to the study of seawater and freshwater DOM Water source and isolation method a Column Mobile phase Ref.

Reversed-phase liquid chromatography
Seawater, SPE   Tables 1-3. organic matter, in particular lignin, was oxidised by CuO and separated using a Lichrosphere 100 RP 18 (4 Â 250 mm, 5 mm particle size) column and a mobile phase composed of phosphate buffer, MeOH and MeCN. Lignin-derived phenols were monitored through UV adsorption at 280 nm and identity conrmed by their absorbance spectra (230-340 nm). Together with the aid of carbon isotope analysis, this method underlined the presence of distinctive chemical patterns when analysing organic matter of marine origin and terrestrial origin, allowing for the comparison of samples from different collection points.
Parlanti et al., also used RP-LC with diode array detection (DAD), to compare the proles of DOM from marine and freshwater sources (Table 4). 139 Using a water-MeCN gradient, the authors were able to identify compositional differences (and similarities) between the two types of DOM sample, and were ultimately able to use the separation achieved to divide their DOM into multiple fractions according to polarity. These fractions were subsequently further separated by means of capillary zone electrophoresis (CZE), providing orthogonal selectivity to the RP-LC, with the authors suggesting CZE demonstrates considerable potential for DOM proling and characterisation of DOM of varying origins (see Section 2.3).
In a similar study, Simpson et al., also investigated the use of RP-LC for DOM fractionation, here using a deuterated water-MeCN gradient, again with DAD, monitoring at 280 nm in order to detect compounds enriched in double bonds and aromatics (Table 4). 140 The chromatograms recorded at this wavelength (for different freshwater sources of DOM) included large predominantly unresolved series of peaks, providing three fractions, and a separate more retained series of co-eluting peaks (fourth fraction). Each of these fractions was subsequently analysed by NMR. From the four RP-LC fractions obtained, a total of 150 NMR spectra were collected. The spectra from the early eluting fractions contained sharp aromatic peaks of relatively polar species (phenols and/or aromatic acids), which were eluted under almost purely aqueous conditions. The NMR spectra from the following fractions were dominated by broad signals, indicating an aggregation of co-eluting species. However, despite the broad proles, differences could be identied between the spectra, indicating that the chromatography provided a certain degree of separation.
Koch et al., investigated the impact of pH (and the use of mobile phase buffers) upon the RP-LC separation of DOM, proposing a 'bufferless' pH-neutral water/MeOH gradient (Table 4). 141 As MeOH can act as both proton acceptor and donor (whereas MeCN can only be a proton acceptor), MeOH can undergo polar or hydrogen bonding interactions with solutes, particularly when the pH of the mobile phase is neutral, so that any secondary interaction is prevented. Koch et al., thus found the absence of buffers and neutral pH approach resulted in more resolved peaks of the water soluble components (Fig. 4), whereas lower pH separations caused extensive co-elution. However, despite the partial success of this approach, the authors were clear to point out the necessity to further reduce the complexity of DOM samples prior to RP-LC and propose the use of a multi-dimensional chromatographic approach involving SEC.
Hutta and co-workers have extensively studied terrestrially derived organic matter (i.e. humic acids and lignin) and, based on their previous studies, which involved the use of a mobile phase gradient composed of a phosphate buffer and dimethylformamide, collected individual fractions of soil-derived humic acids from RP-LC with uorescence detection. These were subsequently further separated by means of SEC (also with a phosphate buffer and dimethylformamide gradient and uorescence detection). 54,[142][143][144] In both chromatographic steps dimethylformamide was chosen for its proven solvating power with regards to humic acids, polyelectrolytes and humic substances. 142,143 This off-line 2D method provided increased resolution of certain compounds in the second dimension. However, a notable drawback of this procedure was the high boiling point of the mobile phase, which renders this method unsuitable for universal forms of detection such as MS, evaporative light scattering detection (ELSD) or charged aerosol detection (CAD). The combination of RP-LC and MS detection is potentially well suited to the analysis of DOM or classes of compounds within DOM (i.e. humic or fulvic acids). The combination of the various chromatographic methods available, and the molecular speci-city of MS detection, is essential for mining molecular denition within such complex mixtures. However, despite MS detection providing an additional dimension in achievable resolution, it should be noted that challenges remain in the interpretation of the resulting spectra which are characterised by multitudes of molecular adducts or ions derived from the thousands of compounds characterising DOM. [145][146][147][148][149][150] Further to this, it is oen difficult to identify and isolate signals derived from artefacts, which can derive not only from the extraction or chromatographic stage, but also from the ion source. [151][152][153] Following collection of mass spectra, potential elemental formulae are assigned to the acquired monoisotopic mass of each molecular species, within the mass accuracy limits of the instrument used. 44,131,154,155 Kendrick mass analysis plots and van Krevelen diagrams are commonly used in describing DOM composition and are a valuable aid in simplifying the enormous amount of data generated from these experiments. 149,156,157 Kendrick mass defect highlights the presence of homologous series differing from each other by the number of CH 2 groups and is usually plotted as function of nominal Kendrick mass. Within this representation, ions belonging to the same homologous series have the same Kendrick mass defect but different nominal Kendrick mass and are positioned along a horizontal line on the plot. This representation is oen used in conjunction with van Krevelen diagrams, where H/C ratios of each identied molecule are plotted against the respective O/C ratios. These diagrams are useful in assessing the presence of various classes of compounds within DOM. However, it must be highlighted that different molecular formulae can be characterised by analogous H/C and O/C ratios and therefore be overlaid within such plots. 154 By using these kind of plots, DOM from different sources can be readily compared, with considerably more detail than possible using simple UV or uorescence based detection. 43,75,149,158 More recent studies have begun to explore greater possibilities in MS detection for DOM characterisation. These include for example the use of tandem MS and hydrogen-deuterium exchange (H/D exchange) experiments. 34,35,[159][160][161] As most of the MS and MS n experiments are difficult to interpret, particularly identifying isobaric losses and the rearrangements that can occur during fragmentation, tools such as H/D exchange can help to distinguish functional groups such as hydroxyls from ethers or carbonyls. 35,162 Additionally, due to the tendency of metal ions to form primarily even-m/z complexes within DOM, and in particular humic substances, Mg 2+ , Be 2+ , Cr 3+ and Mn 2+ have also been used to further simplify mass spectra. [163][164][165][166][167][168][169] The resulting even m/z complexes stand out in the spectrum and can directly be characterised by molecular formulae assignments or tandem MS experiments. 166,[170][171][172] On the basis of previously developed HR-MS methods, 42,156 Stenson et al., targeting humic substances within a Suwannee river fulvic acid standard, 162 presented the separation of DOM isomers through RP-LC-HR-MS. Ions with identical formulae were found within different chromatographic fractions and analysed using the above H/D exchange protocol, providing for isotope differentiation. Structural isomers are different in the total number of exchangeable hydrogens and in the efficiency of each exchange. Spectra were obtained through ion molecule reaction, which avoids fragmentation during the ionisation process, rendering data interpretation more challenging due to the overlapping of fragmentation patterns. 173 Spectra appear more resolved and less ambiguous, however ion molecule reaction is time consuming, requiring six minutes per scan. This means that only a small portion of sample can be processed. The investigated isomers not only had different retention times on the RP-LC chromatogram, but also reported different H/D exchange, which is evidence for the rst isomeric fractionation of DOM.
In  resolution of individual molecules, but demonstrating a peak maximum shiing towards increasing retention times for samples collected progressively further offshore. However, MS detection in this instance was able to further highlight how DOM also showed considerable variations due to photochemical modications. Average MS spectra were used to ascertain that the estuary DOM displayed a bimodal mass distribution with an intensity-weighted average of 0.895 kDa, whereas 1.13 kDa was recorded in the case of terrigenous DOM. However, aer irradiation, the latter more resembled the composition of estuary DOM and its intensity-weighted mass distribution decreased to 0.885 kDa, with a large fraction of UV-absorbing compounds not being detected aer photodegradation.
In 2009, Reemtsma reviewed the issues encountered when coupling RP-LC to MS. 176 Specically, column overloading and signal to noise ratio issues were noted as limitations of the technique. As a solution to these problems, the author proposed the application of RP-LC fractionation followed by direct infusion to HR-MS, as already suggested by Koch et al. 141 As previously mentioned, this work proposes the SEC pre-fractionation of DOM extracted using SPE according to Dittmar et al. 57 The work underlines the complementarity of RP-LC and HR-MS, demonstrating that within each of the four fractions collected from RP-LC, approximately 400 to 900 different molecular formulae containing C, H and O were assigned. Single molecules were found to be fraction-specic, therefore allowing the technique to be usable in targeting potential biomarkers within DOM.
In a more recent study, Liu et al., used RP-LC with UV detection to obtain three to four fractions (according to the sample), which were rst concentrated and subsequently injected into HR-MS for further characterisation. 177 Within this work, only peaks with UV response at 254 nm were considered for collection, and MS and MS/MS analysis. MS spectra showed a peak distribution in the range of m/z 200-700, with peaks existing mainly at odd m/z and consisting of clusters of peaks at each nominal mass, which is consistent with earlier ndings showing analogous m/z distributions. 178,179 Minimally retained hydrophilic fractions typically included low MW compounds (<0.4 kDa), whereas most of the sample was characterised by hydrophobic components. This procedure reports the resolution of hundreds of compounds, however, as DOM was extracted through C 18 -functionalised silica SPE disks, the following chromatographic procedure represents a repetition of the extraction procedure, as an analogous stationary phase is used during RP-LC fractionation. 22 For this reason, many authors have prescribed the direct analysis of SPE extracts (obtained from PS-DVB and C 18 -functionalised silica) via direct infusion HR-MS. 43,152,[155][156][157]177,180,181 Such a direct approach is less time consuming, can provide increased signal to noise ratios, and freedom from artefacts derived from the chromatographic procedure. 182 However, in accepting the resolving power of MS detection, one has to also acknowledge potential biases originating from the ionisation source, which can be more efficient for certain classes of compounds over others, and the additional risk of insource fragmentation. 176,183 For example, ESI, which is the most popular ionisation source in DOM analysis, is particularly suited for ionic, high polarity compounds. Singly or multiply charged ions can be generated, and the number of charges retained by a particular analyte depends on factors such as molecular size, chemical composition, the solvent composition and the instrument parameters. In general, for molecules with mass lower than 2 kDa ESI generates singly, doubly, or, in some cases, triply charged ions, while for molecules with mass greater than 2 kDa, multiply charged ions are more common. 22,75,118,162,182,184 Atmospheric pressure chemical ionisation (APCI) can also be found within DOM MS analysis, especially when attempting to target low polarity compounds. This technique generally provides singly charged ions: multiply charged species are not commonly observed as the ionisation process is more energetic if compared to ESI. 159,185,186 Matrixassisted laser desorption ionisation (MALDI) has also been used in DOM analysis but this so ionisation technique mainly targets large molecules (up to 300 KDa) such as proteins and peptides, therefore not providing any information on the bulk of DOM. Thus currently there is no universal ionisation technique capable of unbiased ionisation of all of the classes of compounds within DOM. The ion source of choice commonly represents the best compromise in attempting to target the vast majority of DOM compounds. As already discussed by several authors, best approach is then to combine different HR-MS analysers, in order to complement the different kind of information that is delivered. 39,187,188 2.1.3. Size exclusion chromatography. Size exclusion chromatography (SEC) separates compounds on the basis of hydrodynamic molecular size. Samples are injected onto a column containing a porous gel stationary phase, within which small molecules can access more of the internal pore volume than larger molecules, which are excluded. SEC is used for MW based fractionation but can sometimes display selectivity bias due to the effects of the secondary solute-gel interactions. For example, hydrophobic compounds can adsorb onto the gel surface, resulting in secondary retention, and an articially low MW. Equally, electrostatic repulsion will result in articially high MW, as the charged species are eluted faster than would otherwise be the case. Due to these issues and inter-sample variability, MW ranges obtained from SEC are oen variable, and should not necessarily be considered as particularly accurate. For example, taking two reports for freshwater DOM based upon the use of SEC, Pelekani et al., report MW ranging from 0.5 to 30 kDa, whereas Landry et al., report from 0.3 to 14 kDa. 189,190 2.1.3.1. Secondary interactions and choice of mobile phase. SEC has been widely used in the separation and fractionation of DOM and terrestrially-derived organic matter (i.e. humic and fulvic acids). 191,192 Everett et al., used SEC to characterise freshwater DOM isolated by tangential ow UF (Table 4). 193 The use of SEC on samples obtained using UF (1 kDa polysulfone membrane) proved the technique successfully isolated the >1 kDa fraction. However, this work also highlighted some of the limitations of SEC for DOM fractionation. Applying similar conditions to those proposed by Chin et al., 194 the SEC method used involved the addition of 0.1 M NaCl to the 2 mM phosphate buffer (pH 6.8) mobile phase to reduce secondary electrostatic interactions between the sample and the stationary phase. Chromatograms obtained under these conditions indicated several size fractions to be present within DOM samples, but these were very poorly resolved, presenting as a broad coeluting peak. Interestingly, the authors did report that the presence of divalent cations within the DOM sample increased the observed MW distribution for DOM samples, which was lower following proton-exchange. This latter observation has obvious implications for the size fractionation of DOM following sample acidication.
Minor et al., employed SEC with a 100 mM phosphate buffer (pH 7) to analyse DOM samples extracted from UF (molecular weight cut-off: 1 KDa). 64 Distinct variations were observed within apparent molecular size distributions from different samples, especially at high MW. High MW fractions were found to be rich in oligo-and polysaccharides containing aminosugars, deoxysugars, and methylated sugars, whereas the low MW portion was enriched in hexose containing oligosaccharides (Table 4). Schwede-Thomas et al., also used a NaCl containing mobile phase, similarly to Everett et al., however the phosphate buffer concentration was ten times higher. 59,193 No size exclusion chromatograms were shown, however the authors observed MW distributions similar to those reported in previous works, and noted that terrestrially derived DOM possessed higher MW compared to their Antarctic counterparts. 194,195 As underlined by Piccolo et al., high MW materials can sometimes be artefacts commonly observed within SEC separations of terrestrially-derived DOM. 191,196 According to the authors, humic substances in solution result from the aggregation of heterogeneous moieties, which are held through hydrogen bonding and hydrophobic interactions. These can unpredictably interact with the stationary phase of the column in use, therefore rendering any measured MW distribution tightly dependent on the SEC column used. The authors underline that, due to the indenite primary chemical structure of compounds such as humic substances, SEC can only provide approximate MW values, which resulted in the conclusion that SEC is more useful to compare changes in molecular sizes between different samples.
Pelekani et al., in their study comparing SEC with ow eld -ow fractionation (FIFFF) for freshwater DOM size characterisation, also pointed out the signicance of secondary solutesorbent interactions in SEC of such samples. 189 Using a series of carboxylated organic dyes as test solutes, signicant evidence of both hydrophobic and electrostatic interactions were observed using a bonded silica gel SEC column, the latter of which were not eliminated through the use of a 0.1 M NaCl mobile phase. However, despite these limitations, reasonable agreement between the two independent size characterisation approaches for drinking water samples was achieved, providing validation of the technique for such applications.
Müller et al., compared two separate SEC columns for DOM fractionation (Superdex 75 HR10/30 and TSK HW-50 columns), each used with 25 mM phosphate buffer (pH 6.8), ionic strength 0.04 M, as mobile phase without the addition of NaCl (Table   4). 197 The method provided a slightly improved separation of freshwater DOM, and enabled the collection of multiple fractions, which were then re-injected onto the SEC column. The reinjected fractions showed well dened Gaussian peaks of distinct elution volumes, which remained reproducible for periods of up to a week following fractionation. Both columns provided similar well dened fractions, which did support the hypothesis that molecular size was the dominant separation mechanism. However, collectively the peak area for the individual fractions was less than that recorded for the original sample, which suggested degree of irreversible adsorption of hydrophobic material.
Her et al., conrmed that signicant ionic interactions occur in SEC when the ionic strength is low. 198 At ionic strengths greater than 0.2, while such effects are suppressed, other secondary hydrophobic interactions remain. Aromatic species within DOM appear to be associated with most of the irreversible adsorption issues, with retention times shis also observed. The columns evaluated within this study enabled the separation of species of size range 1-6 kDa (Biogel P6), 1-30 kDa (Protein Pak 125), and up to 5 000 kDa (TSK 125). Given the uncertainty and variability of MW distributions within DOM, the most appropriate choice was found to be TSK 50S, as conrmed in a following publication. 199 However, the type of stationary phase should also be considered. Biogel P6 is characterised by a polyacrylamide stationary phase, Protein PAK 125, by a silica-based stationary phase, and TSK-50S, by a hydroxylated organic stationary phase (Table 4). Both TSK 50S and Protein PAK 125 stationary phases are highly hydrophilic and therefore susceptible to hydrogen bonding interactions. This kind of secondary interaction can affect selectivity, causing hydrophilic compounds to be more retained, independently by their MW. On the other hand, a polyacrylamide stationary phase (Biogel P6) is more hydrophobic and for this reason, secondary effects from hydrogen bonding are less profound. The ndings from Her et al., were also conrmed by Nissinen et al., who assessed that adsorption interactions and charge exclusion are altered by pH and ionic strength. 200 Such observations led Her et al., to optimise their chromatographic method, and although peaks were not fully resolved in a subsequent study, DOM was separated into ve fractions according to MW. 199 The issue of secondary interactions has been reported in the majority of studies employing SEC to DOM characterisation (Table 4). 201,202 According to Specht et al., secondary interactions take place regardless of whether the stationary phase is a polymer or silica based. 203 Within this study, elution volumes obtained from two different columns were compared. The rst column was a TSKHW50S, with a hydrophilic stationary phase obtained from the copolymerisation of ethylene glycol and methacrylate polymers, whereas the second a TSK G2000SW, with a bare silica stationary phase. Three categories of compounds were tested to understand the type of secondary interactions, namely amino acids, alcohols and carboxylic acids. Within these sets of experiments, performed using a phosphate buffer as the mobile phase (pH 6.8), both polymer and silica based columns were found to display hydrophobic interactions. Alcohols and monocarboxylic acids showed an increased elution volume which was proportional to the number of carbon atoms, whereas aromatic compounds were found to be strongly retained by both types of stationary phases.
Similar considerations were noted in the work of Reemtsma et al., who added MeOH to their SEC eluent (80/20 NH 4 HCO 3 / MeOH) to separate the fulvic and humic acid fractions of DOM (Table 4). 204 Ammonium bicarbonate was used as the buffer, to decrease the secondary electrostatic interactions, here being sufficiently volatile, to facilitate direct coupling of the SEC column to ESI-MS detection.
Persson et al., compared MW distributions obtained through SEC-UV and RP-LC-ESI-MS. Lower MW molecules with exposed carboxylic groups were more readily ionised in MS, whereas, as previously mentioned by Her et al., higher MW compounds with greater specic absorbance in the UV (280 and 254 nm) appeared to be over-represented in SEC-UV. 198,205 Further fractionation of DOM by using two preparative scale columns connected in series (and a NaCH 3 CO 2 containing mobile phase), provided eight size-based portions of DOM. 206,207 Pyrolysis-GC-MS analysis of the so-acquired fractions isolated single compounds. In a recent study by Woods et al., the coupling of SEC to NMR was reported (using an 0.1 M NaCl and 0.03 M NH 4 Cl mobile phase, pH 11) (Table 4). 47,132 Two 7.8 Â 300 mm columns (size exclusion limits ¼ 1-80 kDa for the rst column and 0.5 to 10 kDa for the second) were used in series in order to obtain three fractions of DOM according to size, prior to characterisation using NMR. The rst fraction was enriched in carbohydrate and aromatic-like structures, whilst the second was representative of CRAM, and the third of MDLT. Even though the chromatography in this case could be improved, for the rst time the authors demonstrated the partial separation of CRAM and MDLT. This was also the rst SEC method reported applying a highly basic mobile phase to avoid any sample protonation. Due to the aforementioned issues regarding secondary interactions between sample and stationary phase, SEC is here only used as a means to sizefractionate DOM. Concerns regarding accuracy of any MW prediction meant no specic conclusions on DOM molecular weights were drawn.
Kawasaki et al., also used a phosphate buffer mobile phase (pH 6.8), with an OH-functionalised stationary phase (Table  4). 208 The method used a smaller particle size (5 mm) column with a reduced injection volume (100 mL, representing a 20-fold decrease if compared to the study from Her et al. 199 ). The optimised separation provided the fractionation of DOM within 35 minutes, and the authors reported higher sensitivities compared to previously reported methods.
On the basis of the methodology reported within Peuravuori et al., Romera-Castillo et al., further explored the fractionation of DOM and its variations according to pH. 206,209 This study again conrmed the presence of supramolecular structures characterised by assemblies of small molecules with analogous uorescence properties. Aer obtaining eight SEC fractions from DOM, uorescence studies showed most of the molecules along a MW continuum, indicating similar nature, wide size distribution and a maximum uorescence signal within the 0.18 to 2 kDa range.
To investigate the effects of ionic strength (buffer concentration) and pH of the mobile phase, Sanchez-Gonzalez et al., 107 investigated an ammonium sulphate/ammonium dihydrogenphosphate buffer (pH ¼ 6.5) at increasing concentrations (5.0, 25, 50 and 100 mM). Improved fractionation of DOM was obtained at lower buffer concentrations (25 mM), while when higher buffer concentrations were used, the compounds appeared to be more retained, probably due to increased hydrophobic interactions. However, when different pH was tested, within the range 6.0 to 7.5, DOM fractionation was not dramatically affected (Fig. 5).
In 2012, two separate LC Â LC approaches were explored by Duarte et al., providing new information on MW distributions of humic and fulvic acids from the International Humic Substances Society. 210 Within the rst method, a C 18 -functionalised silica column (4.6 Â 100 mm, 5 mm particle size) was used in isocratic mode (20% MeCN in water), prior to a second dimension SEC separation (polyhydroxymethacrylate copolymer stationary phase, 8 Â 250 mm 10 mm particle size), also in isocratic mode (11% MeCN in 20 mM NH 4 HCO 3 , pH 8.0). In the second approach, the rst dimension comprised an alkyl diol functionalised mixed mode HILIC column (4.6 Â 100 mm, 5 mm particle size) operating in reversed-phase mode (10% MeCN in 20 mM CH 3 COONH 4 at pH 6.0). As within the RP-LC Â SEC method, SEC in isocratic mode (11% MeCN in 20 mM NH 4 HCO 3 , pH 8.0) was also used as second chromatographic dimension. Three detectors were used in both approaches: UV (254 nm), uorescence (excitation: 240 nm, emission 450 nm) and ELSD. Both methods reported comparable results, with 2D chromatograms still showing fractions not completely resolved. However, those eluting at higher retention times within the second dimension seemed to be related to more hydrophobic moieties. The authors also underline the importance of method optimisation (i.e. mobile phase compatibility, modulation period and separation time), and found that, within SEC, MeCN contents higher than 20% provided poorer resolution and a move towards higher retention times.
2.1.3.2. The choice of SEC calibration standards. Correct calibration standards for MW determinations using SEC are critical. However, in the specic case of DOM, as a complex mixture of thousands of unknown molecules, it is clearly very challenging to select the appropriate standards. As already mentioned, the difficulty in determining precise MW distributions is also related to the type of stationary phase, as secondary interactions with the sample can occur. Therefore, Conte et al., point that the molecular weights determined by SEC should be regarded as relative to the system being used (i.e. type of sample and employed chromatographic conditions) rather than absolute values. 191 Protein-based standards (up to approximately 80 kDa) were used in the study from Nissinen et al., whereas both proteins and polysaccharides were used in the work of Minor et al. 64,200 However, each of these calibration standards only represent one of the many classes of compounds within DOM, and for this reason can be considered as non-representative of the whole organic mixture. When determining MW from different SEC DOM fractions, Minor et al., prepared two calibration curves, one obtained by using the protein-based standard and a second by using the polysaccharide-based standard. However, considerable variations were observed. For example, for the highest MW fraction, a MW of 660 kDa was estimated when using the calibration curve from the protein standard, as compared to 200 kDa in the case of the polysaccharide standard. 64 Polystyrene and sulfonate standards from 1 to 35 kDa are the most widely used SEC calibrants. 132,190,198,205,[208][209][210][211][212][213] Within some studies, other side compounds such as glycerol, acetone, chlorobenzoic acid, polyethylene glycol, blue dextran and salicylic acid are added to extend the MW range. 61,190,198,205,208 Although once again, the use of these kind of standards, given the variety of material within DOM, represents a compromise. Similar considerations can be applied to the work from Yan et al., where the selected calibrant was poly(ethylene glycol) (MW range: $0.1 kDa to $50 kDa), 214 and although concluding an apparent DOM MW range from 3 to 16 kDa, also reported measurement errors ranging from AE10% to AE30%.
In order to overcome this issue, Peuravuori et al., used widespread classes of compounds with MW from 0.265 kDa to 169 kDa (pyridoxal-5-phosphate, a guaiacylglycerol-b-guaiacyl ether derivative, sucrose, sodium deoxycholate, sodium taurocholate, bierol, trypan blue, cyanocobalamin, tannic acid, polystyrene-sulfonates, polyethylene glycol, ribonuclease A, chymotrypsinogen A, ovalbumin, albumin and g-globulin). 206,207 These compounds resemble many classes of molecules present in DOM, however, within this extensive list of compounds, no terrestrially-derived compounds are present.
A number of studies have used humic and fulvic acid standards from the International Humic Substances Society with SEC. 58,204,[215][216][217] According to Huber et al. and Averett et al., nominal average MWs for these class of compounds are 0.711 and 1.066 kDa. 58,217 However, due to the nature of humic and fulvic substances, which are themselves a very complex mixture of compounds, and the aforementioned secondary interactions occurring in SEC, the resulting MW estimations are only indicative. Despite this, the aromatic and polycarboxylated nature of humic and fulvic acids, which resemble some bulk properties of DOM, together with the standards proposed by Peuravuori et al., could be the most suitable and comprehensive model mixtures to aid in the estimation of DOM MW ranges. 206,207 2.1.4. Hydrophilic interaction liquid chromatography. Hydrophilic interaction liquid chromatography (HILIC) is a mode of liquid chromatography developed for the separation of polar solutes. It involves the application of a polar stationary phase, and a mobile phase with a high percentage concentration of an organic solvent, typically MeOH or MeCN. Theory has it that this combination provides a 'water rich' layer upon the surface of the polar stationary phase, which acts as the true stationary phase for partitioning based retention. However, solutes are oen retained according to a mixed partition/ionexchange mechanism, and are eluted in order of increasing hydrophilicity. For complex mixtures such as DOM, further interactions can also contribute to observed selectivity, such as hydrogen-bonding, dipole-dipole interactions, and hydrophobic effects. [218][219][220][221][222] The rst application of HILIC to the fractionation of DOM was reported by Woods et al., who collected fractions from their HILIC based separations for molecular characterisation using HR-NMR. 47,133,134 In their initial studies, the group employed a diol functionalised silica column to generate up to 80 DOM fractions. 133 Considerable co-elution between fractions was evident, however with greater retention, increasingly hydrophilic solutes were detected. Typical CRAM and MDLT-like components were eluted in decreasing polarity order along the entire chromatogram, demonstrating a wide diversity of chemical-physical properties within these classications. Carbohydrates were found to elute towards the end of the chromatogram. Fig. 6 shows the HILIC separations of a freshwater DOM sample (Suwannee River) recorded using both UV absorbance DAD and uorescence detection.
More recently, in order to further improve chromatographic resolution, Woods et al., employed a two-dimensional chromatographic approach (HILIC Â HILIC) coupled with NMR 134 for the characterisation of fractionated freshwater DOM collected aer isolation with ultraltration. The column employed as the rst chromatographic dimension was the same as that used in previous mono-dimensional experiments, 133 however, in the second dimension a normal-phase bare silica column was applied. Although not completely orthogonal in selectivity, some improvement in DOM fractionation appears to have been achieved (no two-dimensional chromatograms were shown), as less complex NMR spectra for each fraction were reported.
2.1.5. Ion exchange chromatography. Ion exchange chromatography (IEC) has seen only limited application for the actual separation and/or fractionation of DOM, but has rather seen use in the separation of specic classes of compounds, most notably carbohydrates. Combined with pulsed amperometric detection (PAD), IEC is a common approach to quanti-cation in the analysis of sugars in seawater. [223][224][225][226][227] The direct IEC of seawater samples has oen proven challenging due to the high salinity levels, although sample pretreatment, such as desalting using membrane dialysis, or ion-exchange resins, can be applied. 228 Kaiser et al., developed both RP-LC and IEC methods for the quantication of amino acids, amino-and neutral sugars in oceanic POM, high MW DOM (1-100 nm), and low MW DOM (<1 nm), obtained from varying depths. 227 The developed IEC method used a CarboPac-PA1 anion-exchange column for the separation of amino-and neutral sugars under isocratic conditions (see Table 4). The study reported the concentrations of these small biomolecules fell sharply with depth, accounting for 55% of organic carbon in surface POM, but only 2% of organic carbon in low MW DOM in deep water, suggesting an upper ocean source and rapid microbial turnover.
Repeta and Aluwihare isolated monosaccharides from high MW DOM via acid hydrolysis, and desalting using a Biorex 5 anion exchange resin, with further fractionation of the collected neutral sugars using silver ion chromatography (see details below). These fractions were then further separated using two amino functionalised columns (Hamilton PXP-700) connected in series, for collection of individual sugar peaks for off-line compound-specic radiocarbon analysis. 229 More recently, Sandron et al., 230 reported the use of IEC-PAD to investigate dissolved neutral sugars and their microbial conversion in both articially prepared and naturally occurring freshwater and seawater DOM. Using a CarboPac-PA1 column and gradient elution with a KOH eluent, chromatograms for each sample, both natural and articial, showed obvious similarities, notable a large retained composite peak eluting immediately before the well separated neutral sugars, several of which were readily detectable within the natural DOM samples (Fig. 7). The IEC based separation was used to generate fractions from the articial DOM sample, prior to their further separation and analysis using RP-LC with HR-MS detection, as part of an off-line multi-dimensional chromatographic approach (Table 4). Fig. 7 shows the typical IEC chromatograms obtained for seawater DOM samples collected at 10 and 60 m depths. 230 2.1.6. Immobilised metal affinity chromatography. Immobilised metal affinity chromatography (IMAC) is based upon the application of chelating ion exchange columns saturated with a particular (immobilised) metal ion, to which organic ligands within a sample can interact and form complexes, and are thus retained. The technique can be applied as a SPE technique, or more typically as a column based LC method. The application of IMAC to NOM and DOM fractionation has been based upon the use of what are typically columns in the copper (Cu 2+ ) form to specically copper binding/complexing organic ligands. 231,232 For example, Cu-IMAC based methods have been shown to isolate between 5 and 30% of DOC from soil solutions, with this fraction constituting those species capable of forming stable metal ion complexes. 231 The use of Cu-IMAC for the fractionation of marine DOM was explored by Midorikawa and Tanoue in the mid-90's in their study investigating variation in complexing species with depth. 233 The extracted organic ligands displayed differing characteristics depending upon sampling depth, with surface waters displaying a prominence of ligands rich in both primary amines and carbohydrates. Deep water DOM was characterised by organic ligands low in both these groups, but which displayed strong uorescence.
Specically focussing on humic substances, Wu et al., reported a study comparing IMAC columns of differing metal form, including copper, nickel, cobalt and cadmium, for selective ligand retention (varying also eluent pH and ionic strength). 234 The copper based method was reported to provide the greatest retention and humic substances binding capacity, which supports the common application of copper as the coordinating metal in most applications of IMAC in this area.
Silver ion or argentation chromatography, a close analogue of IMAC, is generally applied to the separation of unsaturated organic compounds, based upon the ability to form a chargetransfer type complex with immobilised silver ions. The unsaturated compound acts as an electron donor and the silver ion as an electron acceptor, [235][236][237][238][239][240] with the stability of the complex increasing with the number of double bonds. Silver ion chromatography is commonly employed in the separation of apolar compounds such as lipid-like materials, and hexanebased mobile phases are employed, with the eluent strength commonly increased using MeCN. 240 However, in DOM characterisation, silver ion chromatography has been applied by several groups for sample pre-fractionation in the study of methylated and neutral sugars.
Panagiotopoulos et al., used preparative silver ion chromatography as a fractionation method for methylated sugars in acid hydrolysed high MW DOM (seawater), prior to fractional analysis using GC-MS (Table 4). 241,242 In this application, the positive charge on silver ions interacts with the partial negative charge on sugar hydroxyl groups, therefore enabling the retention of mono-and di-methylated sugars. Fractionation was carried out using a Supelcogel Ag column with a water mobile phase. Using the combined approach, up to 50 novel sugars were identied, and a trend observed, in which surface waters were enriched in mono-and di-methylated sugars, representing the 64% of the total methylated compounds, whereas deep water samples where richer in mono-methylated 6-deoxy sugars (42% of the total methylated compounds), being derived from predominantly bacteria sources.
As mentioned above, Repeta and Aluwihare isolated monosaccharides from high MW DOM via acid hydrolysis and desalination. 229 The carbohydrate fraction was obtained using silver ion chromatography with refractive index (RI) detection, using two coupled sulfonated PS-DVB cation exchange columns in Ag + form (0.8 cm I.D. Â 30 cm L). Fig. 8A shows the resultant separation, which corresponds closely to that reported by Panagiotopoulos et al., for their similar high MW seawater derived DOM. 241,242 As shown the selective retention of the carbohydrate fractions (eluting between 10-20 min) using silver ion chromatography is very clear.
2.1.7. Counter current chromatography. Counter current chromatography (CCC) describes all forms of liquid-liquid chromatography that use a support-free liquid stationary phase, held in place, generally within an open tubular channel or capillary, by centrifugal forces. 243 High performance counter current chromatography (HPCCC) is a high-performance variant of the above, delivering partition-based chromatography and fractionation of compounds according to their polarity. 244,245 In HPCCC two immiscible solvent systems are employed as the stationary and mobile phases, and depending upon the orientation, either normal-phase or reversed-phase separations can be achieved. In the fractionation of complex samples, such as DOM, HPCCC provides the advantage that all sample material can be quantitatively recovered from the separation, as the stationary phase itself can be ushed from the column and collected/analysed post-separation. In their preliminary study, Sandron et al., recently reported the use of HPCCC in normal-phase mode in an attempt to fractionate DOM (Table 4). 246 The developed separation provided ve fractions which were further analysed by GC-MS or RP-LC with UV detection. In both cases the resulting chromatograms showed differences, supporting the fact that DOM was indeed fractionated into different classes of compounds. Although no HR-MS characterisation was reported, GC-MS fragmentation suggested an analogous molecular skeleton for the vast majority of the fractionated compounds. Complementary analysis via RP-LC with UV detection isolated a number of polar species, which were not detected by GC-MS.
From the above Sections detailing applications of liquid chromatographic techniques to the partial separation and/or fractionation of DOM, some summary points can be made. Both RP-LC and SEC have been applied extensively for such purposes, each providing an initial means of DOM fractionation, albeit based upon differing, and rather general selectivity. In both instances resolution is rather limited due to the complexity of the sample, and in many cases a secondary separation step (e.g. RP-LC fractionation followed by SEC separation, or vice versa) coupled to MS or HR-MS is applied. Clearly, the advantage of MS detection, especially HR-MS is that the technique and data obtained is complementary to the chromatographic separation. LC has the potential to separate isomers, reduces complexity and thus ion-suppression in the ESI source, and makes more of the DOM sample amenable to MS analysis. MS itself provides molecular formulas and conrms changes in composition between LC fractions and/or DOM samples.
Given the general selectivity of both RP-LC and SEC, the application of more specic modes of liquid chromatographic methods for targeted analysis, notably HILIC, IEC, IMAC and silver ion chromatography have been explored, oen applied to pretreated or pre-fractionated DOM. With these methods the potential to better isolate specic classes of compounds (e.g. lipids, sugars etc.), and in some instances individual species exists.

Gas chromatography
Gas chromatography (GC) is only applicable to the separation of compounds that are volatile, or those which can be readily derivatised to volatile species. Prior to separation, compounds containing functional groups with active hydrogen atoms, such as -COOH, -OH, -NH, and -SH, may need to be protected as they tend to form intermolecular hydrogen bonds that can reduce volatility and interact adversely with many GC stationary phases. For complex mixtures such as DOM, with its diverse range of compound polarity, selection of an appropriate stationary phase is difficult, especially if a mono-dimensional GC approach is used. Another complicating issue in the GC analysis of DOM is that many compounds are thermally labile, meaning mode of injection, and injector and column temperature are important parameters to control.
The degradation and derivatisation reactions employed in the GC analysis of DOM fall into three general categories, namely pyrolysis, alkylation, and silylation. Pyrolysis is essentially the cleavage of chemical bonds within large macromolecular structures into smaller and more volatile fragments by the application of heat. The limitation of this technique is the unintentional decomposition of thermally sensitive classes of molecules. 247,248 Alkylation reactions replace active hydrogens from an organic acid or amine with an aliphatic group. This technique is used to transform carboxylic acids into esters, which are more volatile. A common reagent is tetramethylammonium hydroxide (TMAH), which allows the production of ethers, secondary amines and esters. Silylation replaces active hydrogens from acids, alcohols, thiols, amines, amides, enolisable ketones and aldehydes with a trimethylsilyl group, although there are also other silyl derivatives. Silylation reagents themselves (e.g. bis-trimethylsilyl triuoroacetamide (BSTFA)) and silyl derivatives are unstable and must be protected from moisture.
The analysis of DOM by GC is either targeted to certain classes of molecules (i.e. lipids, lignin monomers), or non-targeted, in an attempt to provide a generic screening of the entire organic pool (Table 5). In the majority of published methods, the stationary phases employed have been relatively non-polar (based upon 5% phenyl/95% polydimethylsiloxane, e.g. DB5, VF5MS, RTX5MS, BPX5). More selective stationary phases have been generally avoided, due to the complicated range of chemical functionalities within DOM, which would see many compounds irreversibly adsorbed.
As mentioned in an early review by Aiken et al., 249 one of the rst attempts to use GC in the analysis of DOM (freshwater) was reported by Stainton. 250 This method reported a versatile yet simple extraction approach prior to GC analysis. Volatile species evolved from acidied water samples were collected via a gas stripping procedure with helium ow, the latter being used as carrier to deliver the sample to GC. The extraction efficiency of the method was highly dependent on the stripping time and on the nature of the sample, and applicable only to the highly volatile DOM fraction. Other than predictable co-elution issues, a limitation of the procedure described was the use of polypropylene (PPL) syringes during the gas stripping stage, as these can be a source of potential contamination. 251,252 Due to the complexity of DOM and extensive co-elution, especially in the absence of sample derivatisation, Schulten et al., considered two approaches, namely pyrolysis-eld ionisation MS and Curie-point pyrolysis GC-MS. 253 A 30 m DB5 capillary column was used, characterised as a nonpolar stationary phase, targeting the separation of the mid to low polarity fraction of DOM. The aim of this study was to identify series of marker signals within freshwater DOM, which could allow inter-sample comparison. The obtained GC chromatogram showed fourteen prominent peaks and series of co-eluting compounds ranging from approximately m/z 200 to 500. Despite the authors highlighting the need for further method development (i.e. column selection, pyrolysis and MS conditions), classes of compounds such as benzenes (42 identied structures), phenols (26) and furans (35) were identied, which were further conrmed by following studies. 254,255 Additionally, a wide range of ubiquitous substituted aromatic structures were found, which could not be identied. For this reason, Schulten In a more targeted approach, Mannino et al., used GC-MS (mass range 0.05-0.6 kDa) to determine lignin phenols and lipids, following extraction using an organic solvent (CH 2 Cl 2 ) and TMAH derivatisation (Table 5). 256,257 The extraction technique used by Mannino et al., aimed to isolate the targeted classes of molecules, however, extraction of other DOM constituents, such as complex sterol-like materials, e.g. CRAM and terpenoids, was also evident, leading to substantial coelution, particularly within the rst and middle part of the chromatogram. However, using this method, the majority of lipids were extracted from river estuary samples, including fatty acids, with chain length typically ranging from 9 to 13 carbon atoms. Concentrations of lignin-like material were found to be higher in estuary regions than samples from other coastal regions, with terrestrially-derived DOM (i.e. humic and fulviclike substances) also following an analogous trend. The study conrmed terrigenous DOM is enriched in lignin-like materials, whereas lipid-like materials, consistent with previous studies 30 were found to have concentrations up to 1 mg mL À1 . 30,258,259 Kracht et al. applied pyrolysis to freeze-dried DOM. 260 This study was the rst to employ a combined form of detection involving elemental analysis and pyrolysis gas chromatography mass spectrometry-isotope ratio mass spectrometry (Py-GC/MS-IRMS), in order to correlate mass spectra to isotopic ratios and derive more comprehensive information on the origin of DOM (Table 5). Although using only one form of sample treatment, the authors actually proposed the treatment of the sample with different derivatisation techniques simultaneously. This approach could be used to detect other volatile compounds present in DOM, possibly converted as silyl derivatives, to compare their elution prole and detector responses to those obtained aer thermal pyrolysis.
A limitation of the method developed by Kracht et al., relates to the extraction method employed. Although freeze-drying can provide a potentially uncontaminated extract (e.g. free from plastic-derived materials or artefacts), it is time consuming and requires additional sample desalting if seawater samples are processed. Freeze-drying as a process is also solute dependent, with every class of compound having different freeze-drying requirements, making optimisation difficult, leading to inconsistent dryness across the sample, reduced stability or rehydration.
Ohlenbusch et al., applied SPME with GC-MS to investigate the interaction between DOM and ten halogenated phenols. 109 As with previous studies (Table 5), a DB5.MS column was used. This was chosen for its apolar and aromatic stationary phase (as targeted compounds were phenols). This study revealed the sorption of these compounds to DOM, which was directly proportional to the hydrophobicity of the phenol and inversely proportional to a pH increase. Furthermore, the authors were able to quantify the phenols by using selected ion monitoring mode when processing MS spectra collected in full scan mode.
Aluwihare et al., performed a targeted analysis on two different classes of compounds within DOM, lipids and monosaccharaides, which were separated and identied by GC, with ame ionisation detection (FID) and off-line NMR. 261 Prior to GC-FID, DOM samples were liquid-liquid extracted with dichloromethane and derivatised using BSTFA to detect lipids, whereas acid hydrolysis was used for monosaccharides. 262,263 In the case of carbohydrates, rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose were identied. Unlike the studies from Mannino et al., free lipids were discarded and only ether-or ester-bound lipids targeted. 257,259 It was found that hydrolysable lipids only represented the 2% of the total DOM. The presence of lipid and carbohydrate fractions within DOM was conrmed by means of 1 H NMR, which also identied the presence of resonances corresponding to aromatic and acetate protons.
Lipids were also investigated by Jandl et al., both from seawater and freshwater DOM. The method comprised an extraction in CH 2 Cl 2 /acetone and TMAH derivatisation ( Table  5). 264 GC-MS data were compared to that available in databanks, conrming the presence of a C 14:0 to C 28:0 n-alkyl fatty acid series. The highest concentration was observed by employing RO extraction on freshwater (river) samples (309.3 mg g À1 ), whereas in freeze-dried brown lake water the concentration was nearly halved (180.6 mg g À1 ). This nding not only further highlights the dependence of DOM upon its source, but also the different efficiencies from various extraction methods in use.
Weishaar et al., combined the information from 13 C NMR, UV absorption at 254 nm and TMAH derivatised GC-MS, to focus upon the aromatic portion of DOM (Table 5). 265 Within this study, both electron ionisation and chemical ionisation were used in order to comprehensively screen separated DOM. As already seen, the combination of on-line MS detection with off-line NMR spectra provided a more complete picture of the different classes of compounds within the DOM sample (i.e. proteins, ketones, chlorophyll pigments and aromatics).
Page et al., reported the treatment of a seawater sample with alum in order to remove color and turbidity prior to DOM extraction. 266 The ltered material was then freeze-dried and characterised using pyrolysis GC-MS (Table 5), delivering semiquantitative information on the components of DOM sensitive to this type of sample treatment. The alum-extracted samples were found to be rich in alkylbenzenes, alkylphenols and polycyclic hydrocarbons, whereas the fraction recalcitrant to alum treatment was characterised by the presence of polysaccharidederived molecules. In the specic case of nitrogen containing compounds, alum treatment seemed not to affect the relative abundance of the detected compounds.
In a similar study, Frazier et al., were able to quantify the main compound classes discovered in the work of Page et al., (i.e. fatty acids, carbohydrates and lignin precursors) through TMAH derivatised GC-MS. 267 The signicance of this work arises from the potential to understand the variations these compounds can undergo within different water sources. For example, the chromatograms from four analysed samples showed analogous distributions for carbohydrate-derived compounds, whereas lignin-derived materials were found to be source-dependent and related to indigenous vegetation and local in-stream processes. Fatty acid methyl esters of microbial and plant origins were the most abundant aliphatic moieties.
These were classied into low MW (number of carbons from 8 to 10 and no unsaturations) and high MW (number of carbons from 12 to 18 and no unsaturations). Their proportion showed differences in distribution depending upon the water source.
Multiple detection approaches were also employed by Maie et al., 268 and Templier et al., 269 who compared NMR data to that obtained using TMAH GC-MS (Table 5). Despite the possible contamination due to the fractionation method, the novelty of the Templier et al. study was based upon the combined use of different XAD™ resins to extract DOM, leading to the separation of two fractions with different polarity. This technique simplied the GC-MS chromatograms to an extent that, even if with low intensity, singly resolved peaks were detected (Fig. 9). The DOM sample was also characterised by the presence of large, late-eluting broad 'humps' of unresolved compounds. This unresolved portion of the chromatogram therefore needs to be separated by alternative chromatographic techniques, or via a multidimensional chromatography approach. NMR analysis was also improved by the initial DOM fractionation, and even though extensive spectral overlap was still evident, it was possible to recognise individual well dened classes of compounds.
Quan et al., combined two different GC methods to investigate monosaccharides and lipids contained within DOM, and combined their ndings with monodimensional NMR and UV spectroscopy. 270 The method developed in 2002 by Aluwihare et al., 261 was employed in the determination of monosaccharides, whereas periodate oxidation was employed in the determination of lipids. Aer the oxidation reaction was complete, the lipids were extracted with CD 2 Cl 2 and persylated by BSTFA. Even though the authors underlined the need for more reproducible and precise procedures, the periodate oxidation provided evidence for a carbohydrate fraction which was compositionally different from those analysed according to the method developed by Aluwihare et al. This fraction proved to be rich in both methyl and amino sugars, which seem to comprise 15% of the total carbohydrate content in the sample.
In an attempt to improve resolution, Peuravuori et al., employed a combined chromatographic approach, (LC and subsequently GC), in order to fractionate and then characterise DOM (Fig. 10). 206 The DOM sample was rstly separated into eight fractions using SEC, according to decreasing MW, and then subsequently analysed using GC-MS using two alkylating reagents, namely TMAH, to reveal both esteried and free carboxylic acids, and tetramethylammonium acetate (TMAAc), to determine free carboxylic acids (Table 5). TMAH and TMAActreated DOM fractions obtained aer SEC showed fraction to fraction carryover. However, up to 310 degradation products were detected, of which 185 were identied. These were classi-ed in aromatics (mainly characterised by methyl derivatives of phenols, alkylphenols and phenolic acids) and aliphatics (mainly methyl esters of mono-and dicarboxylic acids). Other generated compounds were furans, cyclopentenones and nitrogen and sulfur-containing organic compounds.
Due to the importance of biologically derived compounds in marine ecosystems, a targeted analysis of crucial biomarkers was conducted by Louchoarn et al., who on the basis of previous experiments 271 applied CuO oxidation with GC-MS/MS, with particular reference to lignin (Table 5). 272 Aer oxidation, lignin was hydrolysed into its three building blocks, vanillyls, syringyls, and cinnamyls, which are readily identied by GC-MS and GC-MS/MS.
More recently, Lang et al., developed an innovative method for the isotopic analysis (d 13 C) of organic samples by using a GasBench plumbing system. Within this approach, water soluble organic compounds were oxidised to CO 2 using potassium persulfate, phosphoric acid and heat (Table 5). 273 The developed gas was delivered through helium ow rstly to an injection valve and then to the GC column which separated CO 2 from other interfering gases (i.e. N 2 O). The puried CO 2 was then analysed by IRMS, with a limit of detection (LOD) of 1.2 mg of carbon. The authors suggest that this method can potentially be useful for determining the isotopic composition of LC-isolated fractions. However, a fundamental prerequisite would be a carbon-free or completely evaporated mobile phase. Another limitation underlined within this work is the possibility that the applied oxidation conditions could be not sufficient to convert refractory materials to CO 2 , limiting therefore its applicability.

Electrophoretic separation techniques
Electrophoretic techniques such as capillary electrophoresis (CE), isotachophoresis, isolelectric focusing, polyacrylamide gel electrophoresis (PAGE) and CZE, in combination with on-line and off-line detection methods such as MS, NMR, UV and uorescence, have commonly been applied to the fractionation, and size and MW determination of many classes of compounds, including proteins, peptides, polymers (both natural and synthetic), and humic and fulvic substances. 274,275 Linear relationships between electrophoretic mobility and MW had been demonstrated in the separation of humic substances, thus paving the way for the size and charge based fractionation of DOM. 276 2.3.1. Gel electrophoresis. Gel electrophoresis with Edman degradation has been widely used as a rst step in the isolation and identication of DNA, RNA, proteins and peptides from DOM. This technology has dramatically enhanced the understanding the role of micro-organisms in DOM pathways. 277,278 One of the rst applications to DOM characterisation using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in combination with Edman degradation and RP-LC, permitted the sequencing of proteins from oceanic waters. 279 Within the electrophoretic separation, up to 30 proteins could be seen as unique bands. These were reported to have molecular masses from 14.3 to 66 kDa. Among these, porins were specically identied. These outer membrane channel proteins of Gram-negative bacteria were found to have molecular masses ranging between 47 and 49 kDa. 279 Within a following study, the same authors could also isolate further classes of proteins from oceanic waters, such as outer membrane protein A (OMP A) homologues, 278 which are known to be resistant to enzymatic digestion. 280,281 Here, proteins were separated and detected using SDS-PAGE in combination with western blotting or direct silver staining. Three major classes of proteins were isolated, namely porine homologues, glycoproteins and lectin-related proteins.
Gel ltration and SDS-PAGE were employed by Schulze et al., to separate proteins from the other organic molecules present within freshwater DOM. 282 Aer silver staining, the gel was cut and subsequent tryptic digests separated and characterised using LC-MS/MS. The obtained spectra were searched against protein databases, and in most cases the sequences obtained were unique to a specic group of organisms. Up to 148 proteins were detected within the surface freshwater DOM, with 78% of them originating from bacteria. It was also observed that the types of proteins present was closely dependent on the season, depth and ecosystem type, as previously observed in a study by Crump et al., who applied denaturing gradient gel electrophoresis (DGGE) to monitor the seasonal variability in RNA samples from Arctic waters. 283 Within a two dimensional approach by Yamada et al., SDS-PAGE and high resolution 2D electrophoresis were applied to the separation of proteins from seawater DOM. 277 This technique resolved up to 412 protein spots from 10 different samples. The most prominent protein bands separated through SDS-PAGE were resolved within the second dimension, highlighting the presence of proteins with analogous molecular weights but different isoelectric points. In particular, two 34 and 39 kDa classes of glycoproteins were classied as isoforms, with the same amino-acid sequence, underlining a further presence of isomers in the DOM pool. 32,33 The glycoforms of the 39 kDa protein were identied as low MW alkaline phosphatase, hydrolase enzymes belonging to the Pseudomonas group, a family of aerobic bacteria which are involved in the removal of phosphate groups from proteins or nucleic acids. Such enzymes play a key role in cellular metabolic pathways and can potentially be targeted as biomarkers to assess the MCP variations within different environmental conditions (i.e. pollution or seasonal change). 278 2.3.2. Capillary electrophoresis. As commented upon in separate reviews (2004 and 2007) by Schmitt-Kopplin et al., 284 and Abbt-Braun et al., 274 the application of CZE coupled to 2D-NMR and/or MS, has greatly helped with the classication of major DOM components, such as humic substances. 274,275,[285][286][287] However, the authors also point out severe unresolved limitations of this approach, mainly related to the presence of artefacts from the chosen separation buffer and the instrumental constraints derived from the complexity of the sample (i.e. extensive co-migration). As underlined by Zsolnay et al., during an electrophoretic separation, the tertiary structure of several DOM components can be modied, to an extent that larger molecules can deteriorate into smaller components. 69 In 2003, Schmitt-Kopplin et al., undertook a comparative study between free-ow electrophoresis (FFE) and CZE-ESI-MS for the separation of a freshwater DOM sample. 284 Prior to this, separation conditions (i.e. pH buffer) were optimised using model compounds which can be found in DOM, such as benzene carboxylic acids. Further to this, for the same set of compounds, MS experiments were run in both positive and negative mode. The authors emphasise how different conditions and instrumental setup can affect analysis, causing for instance, the formation of adducts, multiply charged species and possible fragmentation issues. These phenomena are of high signicance when trying to analyse a mixture of unknown compounds such as DOM. DOM separations (254 nm), obtained using an alkaline buffer, were characterised by a hump with similar m/z distribution. Lower m/z signals presented higher mobility, whereas higher m/z values were found at lower mobility. However, the authors point out that parameters such as size distribution and charge within DOM species is deeply affected by the separation conditions, therefore more experiments at different pH were proposed by the authors.
Due to the limitations identied within the above study, Vogt et al., employed multiple separation techniques, including CZE and capillary gel electrophoresis (CGE), together with SEC, all combined with the information from UV/Vis and FT-IR spectra, uorescence emission spectra (FES), total luminescence spectra (TLS), electron spin resonance (ESR), MS, NMR and potentiometric pH titration. 202 Five samples were processed using this array of analytical methods, with the results collectively highlighting clear differences according to location and seasonal changes. In particular, CZE and CGE were used in analogous conditions (i.e. sodium carbonate buffer at pH 9.3) to determine hydrodynamic radii within DOM components. As the variation in mobility from CZE to CGE is related to the molecular mass, the hydrodynamic radii could be calculated by using molecular mass distributions previously obtained when analysing polystyrene sulfonate standards. However, as discussed previously in relation to SEC, such standards poorly represent many classes of compounds within DOM, therefore, the calculated hydrodynamic radii have to be considered as indicative values.
CZE-ESI-MS has been employed by Hertkorn et al., in combination with CZE-UV (214 nm), NMR and HR-MS spectra, obtaining highly complementary data for seawater DOM collected at different depths. 32,288 A 25 mM ammonium carbonate buffer (pH 9.4 and 11.4) was employed, and although extensive co-elution was also observed throughout the electropherograms (Fig. 11), major similarities in the resulting electropherograms were seen, allowing the authors to conrm, as already proved by NMR spectra, the absence of weakly acidic compounds (i.e. phenols). Within this paper CZE was directly hyphenated to MS, and no buffer removal or sample treatment was reported prior to entry into the electrospray chamber. The presence of the above mentioned alkaline buffer could potentially affect molecular weight distributions and in source sample fragmentation. However, CZE-MS chromatograms corresponded closely to those obtained through CZE-UV, with mass spectra deemed representative of the total DOM composition. The technique also enabled intra-sample comparison and showed that DOM collected at higher depths was characterised by a large fraction of highly charged aliphatic moieties. These compounds appeared to be consistent with CRAM, which were found to be more abundant within DOM collected at higher depths.

Field-ow fractionation
Field ow fractionation (FFF) is a chromatographic technique that usually allows the fractionation of macromolecules according to their diffusion coefficient. 289,290 This technique provides continuous molecular size distribution of macromolecules that can be detected off-line or via on-line coupling with various forms of detection (i.e. DAD, uorescence). FFF is commonly used not only in the fractionation of colloidal organic matter, [291][292][293][294][295] but also in the characterisation and determination of molecular size distribution of the chromophoric fraction of DOM (i.e. humic substances). 295,296 Within early studies, FFF had only been coupled to absorbance detectors. [294][295][296] However, Zanardi-Lamardo et al., on the basis of previous experiments, described the importance of multidetector approaches, and also coupled FFF to a uorescence detector. [297][298][299] Once again the main issue with this technique is the use of polystyrene sulfonate standards as the calibrants, and additionally the surfactants commonly contained in the carrier solution. Similarly to SEC, polymeric materials share little similarity with the complex organic mixture that is DOM, therefore, erroneous MW estimations can be observed. Together with this, as for CE, the presence of surfactants can possibly induce denaturation of components within the sample, leading to a change in the tertiary structure of macromolecules. Such variation is dependent on the surfactant concentration and on the ionic strength of the carrier solution. The higher the ionic strength, the weaker the electrostatic interactions between surfactant and macromolecule.
FFF was employed and complemented with solid-state NMR spectra in a study from Assemi et al., to characterise size and MW distributions of two freshwater DOM samples, which were separated into ve fractions by UF according to their MW (lower than 0.5 kDa, from 0.5 to 3 kDa, from 3 to 10 kDa, from 10 to 30 kDa, and higher than 30 kDa). 300 As the mobility in FFF is related to the particle size, usually the smaller the particles, the faster they elute from the channel. However, the fractograms obtained at 254 nm by using a deionised water carrier, show signicant overlapping between certain fractions. This suggests that these fractions were not separated into discrete size ranges and/or the samples undergo secondary interactions, such as irreversible decomposition of large molecules into smaller units. 69 This is further conrmed by the fact that when FFF (aer calibration with a polystyrene sulfonate standard) was used to determine size and MW, these were found to be smaller than the nominal lter ranges. SEC was used to compare the MW distribution, and showed MW ranges analogous to those obtained through FFF in the case of only one of the two analysed samples.
Moon et al., were the rst to evaluate the effects of ionic strength in FFF carrier solutions on the size determination of DOM, and to provide molecular sizes in terms of hydrodynamic effective size. 301 Such approach was chosen to consider the inuence of diffusion and convection ows during the separation and the interaction forces occurring between DOM and the membrane at the bottom of the FFF channel. To demonstrate the effect of the carrier solution on the separation, KCl and a detergent (FL-70) were used at different concentrations. However, substantial changes in the determination of DOM sizes with increasing ionic strength were not observed, although when FL-70 was used as carrier solution, DOM sizes were lower than those measured when using KCl (20 mM). This was explained by the fact that FL-70 is composed of anionic and nonionic species, allowing the solutes to be more dispersed and preventing aggregation and interactions between sample and the membrane surface. A higher concentration of FL-70 can therefore result in a more rapid elution of DOM, and in a consequent lower size determined by FFF.
Floge et al., used articial seawater as carrier solution (salinity: 32, pH: 8.1) in a further FFF-UV study. 293 The authors observed higher UV absorption in periods following phytoplankton blooms and the year-round presence of colloids (size higher than 18 kDa). Such ndings further conrm the seasonal variability of DOM and that the colloidal species may have a refractory nature.
On the basis of previous experiments, Guéguen et al., also used a polystyrene sulfonate standard and a NaCl solution as a carrier, at ionic strengths analogous to natural waters. 289,302 FFF-UV-DAD and excitation emission matrix (EEM) uorescence were used to calculate the MW distribution of the chromophoric portion of DOM. 303 Pre-fractionation or concentration methods such as UF or SPE were not used, therefore minimising the risk of contamination or additional fractionation. Despite the ubiquitous calibration issues, the mean MW distribution was found to range between 0.8 and 1.1 kDa, depending upon on the sampling location.
Analogous MW ranges (0.68-1.95 kDa) were also found by analysing the chromophoric fraction of DOM by asymmetrical ow eld-ow fractionation (AF4) coupled to uorescence parallel factor analysis (PARAFAC). 303 AF4 was earlier introduced in the characterisation of DOC, coupled to both UV and DOC detection 304 and has the advantage, if compared to symmetrical FFF, of a simpler channel construction and a transparent front plate, where the focusing band is visualised when a coloured analyte is injected. 303 The analysed samples were fractionated, by using a 1 mM NaCl carrier solution, into ve components, which showed humic-like uorophores on fraction 1, 2 and 4, comprising the majority of the total uorescence, and a protein-like uorophore on fraction 5. The method could prove a stratication of such uorophoric material, with surface samples having a higher total uorescence, therefore a higher content in humic substances, if compared to deeper water samples.

Future directions and conclusions
It is hoped this review provides a comprehensive overview of the range and complexity of separation methods applied to this signicant analytical challenge. Clearly, separation science remains central to greater understanding of this complex system, although the breadth of studies included within this review collectively highlight how no one approach individually is capable of providing the immense resolution required for molecular level separations, and this is likely to remain the case for the foreseeable future.
As with most analytical problems, the rst and most significant issue is collection of a representative sample. DOM provides the perfect demonstration of this principle. The difficulties in extracting uncontaminated and unbiased DOM are still considerable. This overriding issue is, in the authors' opinion, much overlooked in the vast majority of papers on DOM characterisation. As with subsequent separation techniques, it would appear multi-dimensional (multi-selective) approaches may provide a more comprehensive solution. How this is achieved practically, particularly for SPE, remains to be seen. Since the compounds constituting DOM are oen at nanomolar or picomolar level, and given the complexity of the sample extraction procedures required, there is always a major risk in sample contamination, e.g. from storage containers, sample preparation (i.e. SPE, UF) and solvents used. This issue too is rarely commented upon and details of process blanks rare in most published studies. For this purpose, articial seawater/ freshwater should be employed and passed through all the extraction and separation procedures that the actual samples undergo. Using this procedure, it is possible to clearly identify and improve the extraction or chromatographic step where artefacts are generated.
DOM fractionation and subsequent separation appears to be a common approach within a great number of studies.
Essentially this is off-line two dimensional chromatography, which attempts to provide some level of resolution prior to highend detection techniques, such as HR-MS or 2D NMR. Woods et al., illustrated this very clearly by employing HILIC Â HILIC separations of DOM, to deliver more resolved NMR spectra. 134 Similar approaches using more orthogonal separation methods are likely to continue to emerge, including on-line multidimensional separation methods, both LC and GC based. However, chromatographically, we will only see peak capacities (resolution) of hundreds of peaks, a long way short of the tens (if not hundreds) of thousands of individual components thought to make up this complex substance. Thus the combination with HR-MS and 2D NMR will remain essential, providing the 3 rd , 4 th and 5 th dimensions required for such molecular level resolution. In particular, HR-MS remains crucial to DOM characterisation, and the rapid development of such technology (including more universal ionisation techniques 305-307 ) will ease, but not delete, the need for ever greater chromatographic resolution. For characterisation of the large number of isomers present in DOM, microgram-level NMR provides a solution, proving that MS and NMR spectra can and should be used to complement each other.
The above comments suggest that much more work remains to be done before obtaining a true understanding of the complexities of this abundant material. 30 However, over the past decade this eld has progressed rapidly, and the solid basis of understanding DOM and its role in the carbon cycle have been laid down by these pioneering studies.