Qualifying label components for eﬀective biosensing using advanced high-throughput SEIRA methodology †

The need for technological progress in bio-diagnostic assays of high complexity requires both fundamental research and constructing eﬀorts on nano-scaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. Nanoparticle induced sensitivity enhancement and its application related to multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are well suitable for these purposes. The potential of diverse fluorophore–antibody conjugates, being chemisorbed onto low-cost gold nanoparticulate SEIRA substrates, has been explored with respect to their spectral discriminability. These novel biolabels deliver molecular SEIRA fingerprints that have been successfully analyzed by both uni-and multivariate analyzing tools, to discriminate their multiplexing capabilities. We show that this robust spectral encoding via SEIRA fingerprints opens up new opportunities for a fast, reliable and multiplexed high-end screening in biodiagnostics.


Introduction
Nanotechnology has an increasing impact on both the design and functionality of biomedical and bio-diagnostic devices for reliable high-performance analysis.High-throughput capabilities require the simultaneous detection of various analytes combined with appropriate bioassay components.Additionally, reduction of the biosensors' cost by using, for instance, low cost nanoparticles (NPs) instead of artificial nanostructures is another important aspect.Various bioassay configurations may be used, such as the immobilization of targeting elements such as antibodies on glass slides coated with various targeting ligands, as microarrays.To design assays for antibodies that function as molecular probes, a selective binding event has to be optimized with respect to the overall physicochemical properties which may influence the successful readout of signals.This constitutes the core of the first design steps to elaborate multi-purpose bio-assay platforms.Bio-assays are standardized tests usually implemented to support multiple intended uses such as the detection of pharmaceuticals, antigens, hormones, or toxins in a complex sample.However, there is usually a long way to go for the establishment of a bio-assay setup, which necessitates preliminary steps such as (i) the development of performance-based methods, (ii) the analysis of biological assays and (iii) their validation.This calls for advanced methodologies and readout procedures.Surface-Enhanced InfraRed Absorption (SEIRA) in combination with statistical tools addresses the challenges of accuracy, specificity, precision, and robustness by providing highly resolved vibrational information of complex molecular compounds.Surface-Enhanced InfraRed Absorption (SEIRA) constitutes an ideal platform to isolate vibrational spectroscopic signatures of targeted and active molecules (e.g.antibodies) of the bio-assay, and challenges the Surface-Enhanced Raman Spectroscopy (SERS) readout. 1,2Dissimilarly to SERS providing sensitive access to Raman-active modes in molecules, SEIRA allows for efficient detection of IR-active modes, thus providing complementary speciation information.][5] The present work addresses one of the design steps of an assay platform -the targeted biolabels consisting, in our case, of fluorophore-labeled antibodies chemisorbed onto gold nanoaggregates (Au NA), acting as nano-antennas for biocompatible, low-cost, amplified SEIRA detection 6 and efficient fast discrimination for appropriate read-out in tunable and pre-selectable spectral ranges. 3ystematic analyses were performed on the model antibody Goat Anti-Mouse IgG and Goat Anti-Mouse IgG conjugated with fluorophores such as fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC) and sulphorhodamine B dye (SULFO) fluorophores.These biolabels adsorbed at/nearby NP-functionalised (silanized) surfaces present additional and enhanced spectral signatures due to plasmonic modifications that were compared with those delivered from non-chemisorbed gold NA films simply incubated with the label components.
Extensive areas of dried sample films were analyzed by IR/SEIRA spectroscopy and the resulting complex hyperspectral datasets were submitted to automated statistical analysis, namely, principal component analysis (PCA).The relationship and dependency between chemical functional groups of various antibodyfluorophore conjugation systems were determined.Consequently, this paper presents profound insights into multivariate discrimination capabilities by direct visualization of derived structural clusters among various biolabel configurations.

Material and methods
For further details, please confer to the ESI.†

Synthesis of fluorophore-labeled antibody conjugates
Different fluorescent dyes that are widely used for biomedical applications were studied.The conjugation of fluorophoreantibody was performed by a covalent linkage between the free residual amino groups of the antibody 9 and the thiocyanate and sulfonyl groups of the fluorophores, 10 and further characterized by MALDI-TOF-MS (Fig. S-2, ESI †).

Atomic force microscopy
Au NP modified low-e-slides were studied using a NeaSNOM (Neaspec GmbH, Germany) in AFM mode for accessing the surface topography of the functionalized substrates and the existence of immobilized Au NPs.Sample areas of 8 mm Â 8 mm and 4 mm Â 4 mm were scanned with a corresponding pixel number of 400 pixels referring to a resolution of 20 nm (8 mmscans) and 300 pixels referring to about 13 nm resolution (4 mm-scans).A platinum iridium V coated monolithic silicon AFM tip with a tip radius of curvature with less than 25 nm (NanoWorld AG, Swiss) was used for probing the substrate surface.Image processing (median alignment, pixel column correction) was performed using Gwyddion 2.2.6 (CMI, Czech Republic).The surface topography depicts Au NA and a single NP to be immobilized on the glass slide.The Au NPs showed spherical morphologies and were in the size range of about 40 AE 3 nm.
2.4 SR-based FTIR micro-spectroscopy 2.4.1 Instrument and data acquisition.The measurements were carried out on the IR beamline at the Metrology Light Source (MLS) storage ring using a Vertex-80v FTIR spectrometer 11 coupled to an IR microscope Hyperion 3000 (Bruker Optics GmbH, Germany) equipped with a 128 Â 128 pixels FPA (Focal Plane Array) detector (pixel size: B3 mm at 15 Â magnification).
For FTIR micro-spectroscopy measurements, the SR source (s x = 670 mm, s y = 183 mm, beam current B170 mA) was focused through an IR cassegrain objective of 15-fold magnification onto the sample films.MIR-spectra (from 3900 cm À1 to 900 cm À1 ) were acquired in transflection mode by co-addition of 128 scans at 16 cm À1 resolution.Background scans were collected before each sample measurement from a region free of sample and rationed against the sample spectrum.The use of synchrotron light has many advantages, thanks to its high brilliance, non-invasiveness, coherence and chemical sensitivity compared to conventional IR sources 12 and this study illustrates its applicability to the acquisition of large spectral datasets with FPA.
2.4.2Sample preparation.For the biolabels, fluorophores and antibody-fluorophore conjugates, 2 ml of the fluorophoreantibody conjugate solution was added to 20 ml of suspended Au NPs, resulting in a final concentration of ca.0.1 mg ml À1 .After an incubation time of five minutes, the conjugate solution was drop-coated onto a low-e-slide, then dried in a desiccator.For comparison, the same amount of conjugate solution without NPs was drop-coated.The NPs (20 ml) were exposed to 2 ml of 10 À4 M FITC, RITC and SULFO solutions, respectively, resulting in a final concentration of 10 À5 M. Fluorophores were prepared in DMF and further diluted in sodium bicarbonate buffer (0.1 M, pH B 9) to a final concentration of 10 À5 M. The antigen Mouse Anti-Goat IgG was pre-dissolved at a concentration of 0.5 mg ml À1 in borate buffered saline (100 mM, pH B 8.2) and mixed with the colloidal suspensions to a final concentration of 0.05 mg ml À1 .For Au NA-based SEIRA substrates, FTIR spectra were acquired in absorption mode in the 22 000-900 cm À1 spectral range using a MCT (Mercury Cadmium Telluride) detector and an InGaAs photodiode (16 cm À1 spectral resolution, 128 scans).As a reference for immobilized Au NPs, a slide coated with APTMS for each discrete concentration was measured.
2.4.3Data analysis: uni-and multivariate analyses.Each FPA measurement dataset comprised 16384 spectra, and due to the limited area illuminated by the bright SR beam the spectra contained regions with no MIR signal allowing for correcting the pixel noise and therefore enhancing the signal to noise ratios.The datasets for each of the 8 sample conditions (Goat Anti-Mouse IgG, Goat Anti-Mouse IgG with Au NA, fluorophore, fluorophore with Au NA, fluorophore conjugate, fluorophore conjugate with Au NA, Mouse IgG antibody with/without Au NA) were reduced to matrices of 550 spectra and loaded into Origin 8G (OriginLab Corporation, USA) for polynomial baselinecorrection.Each of these spectral dataset is issued from a sample region of ca.37 mm Â 96 mm, which is adequate for evaluating the potential of a FPA FTIR set-up as bioassay direct readout configuration.Indeed, the dimension of the surface probed here corresponds to bio-diagnostically relevant areas delivered by sample systems spotted onto microarrays which range typically from 10 mm up to 500 mm. 13rincipal component analysis (PCA): the second derivatives of the full dataset of 4400 baseline-corrected absorbance spectra were calculated and consecutively submitted to the Savitzky-Golay algorithm (5 smoothing points) and vector-normalization before PCA using MatlabR2011a (The Mathworks Inc., Natick, USA) and PLS toolbox (Eigenvector Research, Inc., USA).The principle components (PCs) were calculated from PC1 up to PC4 and illustrated as scatter plots; such a high-order approach has been needed for discriminating certain label components.

Au NA-immobilization on MirrIR low-e-MIR substrates for SEIRA studies
Self-assembled Au NP multilayers have successfully been employed as versatile platforms for molecular sensing approaches. 14Fig. 1 displays the spectral features of Au nanoparticulate multilayers that were generated during the drop-coating procedure and analysed in the 22 000-900 cm À1 region.Due to aggregation of the Au NPs, a red-shift of the plasmon band is induced, and a broader plasmon band appears, extending from the NIR to the MIR region (compare with Fig. S-1, ESI †).The position of the extended plasmon band shows that pre-resonant excitation by surface plasmons in the MIR region can be exploited for the SEIRA studies.This is in accordance with previous observations, 15 where a continuum-like resonance for Au nanoshell array samples was observed in the MIR spectral range.

MIR signatures delivered by fluorophore-antibody NP biolabels
Similar to clinical diagnostics, dried films of serum proteins or antibodies are convenient for FTIR investigations.The study of the secondary structure composition, structural dynamics and/or the study of conformational changes (effects of ligand binding, temperature, and pH) of the antibodies in the liquid phase is not the aim of this work presented here.In the following we discuss the signatures from SEIRA NP-labeled biolabels (such as antibodies and conjugates) that can be used to visualize and ultimately quantify the bound analyte which is not that obvious or detected in the conventional FTIR configuration.Of notice that it is inherent to the SEIRA technique compared to conventional FTIR is the influence of the metal surface that may lead to some spectral shapes, shifts and changes in intensities. 16.2.1 Goat anti-mouse IgG-FITC conjugate.The spectral properties of unlabeled and labeled Goat Anti-Mouse antibodies (Fig. 2A and E) were studied.The signature of Goat Anti-Mouse IgG exhibits two strong IR bands at 1535 cm À1 and 1635 cm À1 that refer to the Amide II and Amide I bands characteristic of proteins 17 (Table S-1, ESI †).In the relatively large wavenumber region (3741 cm À1 to 3278 cm À1 ) the band intensity of the stretching (str.)vibrations corresponding to -NH and -OH  This journal is © the Owner Societies 2015 groups of amino acid residues 18 which form the secondary structure of the antibody is enhanced in the presence of Au NPs (see rhombohedra }).This observation corresponds to IR bands that were detected from other protein-Au bioconjugates. 19The spectrum delivered by Goat Anti-Mouse IgG incubated with Au NPs (Fig. 2B) shows a similar spectral pattern as displayed in Fig. 2A.The band at ca. 3278 cm À1 is attributed to -NH str.vibrations of antibody proteins. 20The signature of FITC is presented in Fig. 2C and exhibits bands referring to str.vibrations (region from 1652 cm À1 to 1496 cm À1 ) of the xanthene ring moiety and external group modes. 21The MIR signature delivered by FITC molecules adsorbed onto the Au NPs is shown in Fig. 2D and comprises str.vibrations of the carbonyl groups of FITC 21 (see asterisks *).The newly occurring band originates from -CQN and -CQC str.vibrations.This can be explained by different binding orientations which are restricted due to the fluorophore-NP incubation procedure during which the chemisorbed FITC molecules tend to orient.In addition, the origin of SEIRA being observed for Au NP-FITC is due to the formation of large colloidal Au NA, presumably induced by the drop-coating process.The NA chemisorbed FITC molecules orient their dipole moments to be in line with the local electric field of the metal NA, resulting in much larger absorption than when they are not in line with the oscillating electric field. 22,23Such larger absorption of FITC molecules can be exploited for building complex conjugates for sensitive multiplexed assay approaches.
An enhancement of the modes in the spectral region between 1304 cm À1 and 1180 cm À1 , as well as at ca. 1458 cm À1 can be observed as well (compare Fig. 2C and D).The Goat Anti-Mouse IgG-FITC signature in Fig. 2E comprises lines which can be assigned to typical bands of FITC (see asterisks *) and bands between 3720 cm À1 and 1103 cm À1 referring to antibody specific signatures.Fig. 2F shows the SEIRA spectrum of the antibody-FITC conjugate recorded in the presence of Au NA.This signature comprises lines which can be assigned to FITC typical bands (Table S-1, ESI †).For example, in the vicinity of the mode at ca. 1643 cm À1 (str.vibrations of the xanthene skeleton) of the unenhanced antibody-FITC conjugate now a broad shoulder being located at 1597 cm À1 appears in the SEIRA spectrum of the antibody-FITC conjugate.This vibration originates from the aromatic -C-C str.vibrations of the xanthene ring skeleton of FITC molecules which was already observed in the SEIRA spectrum of FITC (compare Fig. 2D).This shows that SEIRA is able to detect with high sensitivity fluorophore molecules in their conjugated states along with their molecular orientation.
Considering the intended use of labeled antibodies in immunoassays, the signatures of the targeting counterpart Mouse Anti-Goat IgG were studied.Fig. 2G illustrates antigen specific lines being typical for antibody protein vibrations.The SEIRA signature of the Mouse IgG antibody is displayed in Fig. 2H that shows enhanced modes in the spectral region between 1800 cm À1 and 900 cm À1 .Another possible contribution which may explain the observed spectral differences is due to chemical enhancement.Molecules strongly chemisorbed onto a (noble) metal surface show a larger enhancement than do physisorbed molecules, suggesting a chemical effect between the molecule and the surface due to strong interactions (Au NA-antibody complexation).This mechanism would describe the case that we discuss and correspond to our antibodies that we detect in the presence of Au NA.It is known that the absorption coefficients of chemisorbed molecules are larger than those of condensed overlayers and therefore interactions between adsorbed molecules may also affect the intensity and thus impact and explain the enhancement of some modes.Charge oscillations between molecular orbitals and the metal surface may enlarge the absorption coefficients of adsorbates. 2,24.2.2Goat anti-mouse IgG-RITC conjugate.In order to study the multiplexing capability of further fluorophore conjugates, RITC, a rhodamine derivative, 25 was used for the coupling.Fig. 3B and D display the MIR signatures of RITC and the spectrum of Goat Anti-Mouse IgG-RITC conjugate recorded in the presence of Au NA.Since both fluorophores FITC and RITC have quite similar molecular composition their MIR signatures exhibit the same modes, i.e., the bands at 1172 cm À1 (d C-OH) and at ca. 2941 cm À1 (n as CH 2 vibration) are observed in both MIR fingerprints. 21However, some spectral differences are noticeable.Compared to the spectrum of FITC, the main peak in the RITC spectrum is located at 1589 cm À1 (Fig. 3A) which can be attributed to aromatic str.vibrations.The enhanced intensities of the vibrational modes referring to RITC are highlighted with rhombohedra }.The mode at 1743 cm À1 can be assigned to a -C(QO)H str.vibration. 26The SEIRA spectrum of RITC recorded on Au NA (Fig. 3B) exhibits an enhanced mode at 1589 cm À1 .It is remarkable that the relative intensity of the str.mode of the xanthene ring moiety at 1342 cm À1 experiences an enhancement by a factor of five, which proves again the selectivity of revealing one of the most specific molecular groups of the fluorescent dye.
In the case of RITC measured on Au NA, further new IR vibrations are observed, for instance, at 1666 cm À1 .Further, the mode at 1520 cm À1 disappears which is caused by a change in the molecular orientation.The spectrum of the antibody-RITC conjugate (Fig. 3C) exhibits RITC-typical modes which are also observed in the bare fluorophore spectrum (Fig. 3A).For instance, the mode at 1589 cm À1 originates from aromatic -C-C str.vibrations of RITC molecules. 26In the presence of Au NA, the conjugate signature (Fig. 3B) delivers one broad band at 1589 cm À1 and the mode at 1647 cm À1 is observed as a shoulder being located at ca. 1639 cm À1 (Table S-2, ESI †).
3.2.3Goat anti-mouse IgG-SULFO conjugate.Fig. 4B and D shows the signatures of the SULFO and SULFO-labeled Goat Anti-Mouse antibody, in the presence of Au NA.Compared to the unenhanced signature of SULFO (Fig. 4A), the mode referring to the aromatic -C-C str.vibrations 27 at 1581 cm À1 is enhanced, as the line at 1597 cm À1 is shifted to shorter frequencies.Further modes originate from -C-O str.vibrations of the carbonyl group and -C-C str.vibrations nearby the xanthene ring moiety. 28The SEIRA signature of SULFO (Fig. 4B) shows structural differences in terms of band shifts and its spectral behaviour in the proximity of Au NA is quite similar to FITC (compare Fig. 2D).Further, new bands appear in the long frequency region, for example, lines at 3186 cm À1 referring to -NH str.vibrations at 3325 cm À1 can be observed along with str.vibrations at 3780 cm À1 (-OH). 29In the spectrum of the antibody-SULFO conjugate the band at 1597 cm À1 is red-shifted.However, conjugate typical bands appear at 1412 cm À1 along with modes at 1034 cm À1 and 1242 cm À1 originating from SULFO.The spectrum of the antibody-SULFO conjugate recorded in the presence of Au is very similar to the conjugate spectra recorded without Au NA (refer to Table S-3, ESI †).
The univariate study of these different antibody-fluorophore conjugate signatures shows that the electric field enhancing capabilities differ between Au NPs and the type of antibodyfluorophore conjugate which justifies the systematic approach on the spectral properties of such fluorophore-antibody NP complexes.Additionally, this shows that for the bioassay design, SEIRA induced by NPs provides different vibrational information from fluorophore-anibody NP labels that can be used for building up spectral databases.
Summarizing the results, FTIR spectroscopy combined with SEIRA is an important analytical tool for studying the biochemical composition of proteins such as antibodies.Bands of high interest for biological purposes and labeling procedures can be found in the Amide I/II and ester regions.The Amide I region is traditionally exploited for probing the secondary structures of proteins, as an indicator band for monitoring spectral changes due to alterations in the molecular structure, usually due to external perturbations.Our studies show that complex biolabels that are read out by SEIRA deliver distinct spectra that clearly differ from their unenhanced counterparts, the conventional FTIR spectra (please refer to Fig. S-3, and Tables S-1-S-3, ESI †).

Multivariate discrimination of the signatures of label components
PCA as a powerful chemometric method for finding patterns in complex FTIR datasets enables fast identification and separation of labeled antibodies which are designed for multianalyte detection. 30,31Fig. 5 shows score plots (PC1-PC4) that entail spectral differences.All PCA procedures displayed in Fig. 5A-C (and Fig. 6b) were performed by repetitive experiments (data not shown here, 95% reproducibility).
The PCA displays datasets of the Goat Anti-Mouse IgG that are well separated from the antigen data in PC1 against PC2.The results of PCA confirm that on average 85% of the variance of the spectral datasets, i.e. the discrimination capability up to the third order, could be explained for the fluorophores.Additionally, we decided to put on display all components up to the fourth order to illustrate the options for discriminating different label and/or label components.The scores (PC1 against PC2) of Goat Anti-Mouse IgG are positive and lie in the second quadrant of the variance-weighted room.In PC1 against PC3, the Goat Anti-Mouse IgG antibody data are well separated from the unenhanced spectra.The spectral differences observed in the SEIRA spectrum of FITC (see Fig. 2D) are also reflected by PC1 against PC2.Fig. 5A illustrates that in PC1 against PC2 FITC is separated from the FITC-labeled antibody.With respect to the design of multiplexed immunoassay formats, this shows that the enhancement capabilities of different Au NA labels provide MIR signatures with different spectral patterns.PCA for studying the multiplexing capability of the other fluorophore conjugates containing RITC molecules was  also performed successfully.Similar to the PC scores illustrated in Fig. 5A, a discrimination of the RITC MIR signatures measured on Au NA in PC1 against PC2 was feasible.A variance of about 80% is captured by the components PC1-PC3.The Goat Anti-Mouse IgG-RITC conjugate cluster has quite similar distance towards its unlabeled counterpart as the FITC-antibody conjugate, which is reflected by the good separation in PC1 against PC2.
Compared to all other fluorophore-antibody conjugates discussed so far, the SEIRA signature of the FITC-labeled Goat Anti-Mouse IgG is most similar to the unlabeled antibody sample; for SULFO the distance in PC1 against PC2 is the highest which shows the increase of discrimination capability of the conjugated fluorophores.
The multivariate analysis of spectral features is an important scope for the design and development of bioassay platforms, as functionalization with biolabel components may require several preparation steps before the establishment of a functionally completed assay setup.

MIR signatures of the antibody-FITC conjugate measured on the Au NP modified low-e-substrate
Fig. 6a shows the extinction spectrum and surface topography of Au NPs being immobilized on an APTMS-functionalized substrate (1% APTMS, time of incubation: 24 h, see Fig. 6a,  A-C).A second extended plasmon band is observed in the MIR region and proves the existence of Au NA 32 as for the unmodified glass substrate.This is important in the case of the enhancement capabilities of immobilized NPs as large NA reaching microscale size dimensions of bulk materials might drastically lose their enhancement properties.
The PCA results confirm that on average 85% of the variance of the spectral datasets up to the third order could be explained.PCA of the Mouse Anti-Goat IgG signatures shows a better separation from the enhanced ones (PC1 against PC2 and PC3 in Fig. 6b).The MIR signatures are illustrated in Fig. 6c.Compared to the FITC signature, spectral differences in the enhanced counterpart can be observed, i.e., the band at 1738 cm À1 (compare with Fig. 3) is split into lines at 1726 cm À1 and 1752 cm À1 .The dependence on the substrate configuration and modification is very striking when comparing the antibody-FITC conjugate being adsorbed onto the Au NA on the APTMS-modified slide -the signature exhibits FITC typical bands along with antibody modes (Fig. 6c, C).Compared to the conjugate signature in Fig. 2 where a mode at ca. 1643 cm À1 can be observed, now a new band at 1681 cm À1 (-CQO str.) appears after APTMS modification.This shows that the SEIRA signature of FITC and its antibody conjugate differs from the signatures measured with the APMS-modified substrate and the assay configuration itself.It should be noted that the vibrational modes in the 1600 cm À1 to 1300 cm À1 range can be assigned to both aminopropyl and free amino groups as well.However, no peak at 1491 cm À1 associated with NH 3+ in the SiO À -H-NH 3+ group of APTMS residues 33 is noticeable which shows that this substrate enables the overlap-free detection of amino-group modes delivered by the targeting units themselves.
A tremendous impact of this substrate with respect to its enhancement capability is shown for the antigen Mouse Anti-Goat (Fig. 6c, F) in the region from 1460 cm À1 to 1300 cm À1 .In particular, the mode at 1641 cm À1 is extremely intensified by a factor of about 8.This is in accordance to the calculated dimension of the average enhancement factor for the MIR spectral region as described in ref. 34.

Conclusions
We demonstrate the efficiency of the SEIRA technique to probe the molecular characteristics of different antibody-fluorophore NP conjugates considered in this study.Our measurements show that the spectral signatures of the native antibody Goat Anti-Mouse IgG and the conjugated fluorophores are preserved in the proximity of Au NPs but enhanced by the latter.This implies that the elaboration of specific assay platform spectral databases can be envisaged with SEIRA.Coupled with PCA analysis, this method allows clear discrimination of the functionalized systems, which ascertain their potential for multiplexed bioassay applications and biosensor development of high efficiency with customized responses.Our work highlights the prospective use of NP-assays for biodiagnostics 35,36 and as catalysts for selectively enhancing bio-physiological responses of cells or bacteria.Consequently, SEIRA applications 37 can be expected to show even higher performance characteristics than the conventional/established methods such as SERS. 38s such, a logical extension of our work is to confront the potentialities of SEIRA to discriminate biolabels' spectral features in a real biological environment, which is a subject of another communication.
Altogether, this paper evidences the availability of a novel means in the complex bio-diagnostic micro-assay toolbox to identify the suitability of biolabel systems along with their discrimination potential and their multiplexing capability.Enhancing functionality performances is the key point to obtain higher and targeted screening performance, the ultimate goal of complex bio-diagnostic assay platforms. 39

2. 1
Synthesis of Au NP SEIRA substrates and immobilization of Au NPs on a MirrIR low-e-substrate Au NPs were synthesized according to a modified protocol of Lee and Meisel, 7 yielding an estimated concentration of 1 Â 10 À9 M (ca.7 Â 10 14 NP/L) and characterized by UV/Vis-NIR spectroscopy (see Fig. S-1, ESI †).For the preparation of SEIRA substrates, the immobilization of Au NPs was performed according to the protocol illustrated in ref. 8, using mid-infrared (MIR) low-e-slides.

Fig. 1
Fig. 1 Absorbance spectrum of Au NA showing an extended plasmon band in the mid-infrared region used for the SEIRA experiments.Analysis was performed in the spectral range from 22 000 cm À1 to 900 cm À1 .

Fig. 2
Fig. 2 MIR spectra of (a) Goat Anti-Mouse IgG, (b) Au NA incubated with Goat Anti-Mouse IgG, (c) FITC, (d) Au NPs and FITC, (e) Goat Anti-Mouse IgG-FITC, (f) Au NPs and Goat Anti-Mouse IgG-FITC and the antigen (g) Mouse Anti-Goat IgG and (h) Au NPs and Mouse Anti-Goat IgG measured using SR.Vibrations of FITC are marked with asterisks *.Enhanced vibrations are marked with rhombohedra }.For further details, refer to Table S-1 (ESI †).

Fig. 5
Fig.5PCA illustrated from PC1 to PC4.PCA was performed with vector-normalized second derivative spectra over the spectral range from 3900 cm À1 to 900 cm À1 .PCA clusters are color-coded as follows: Goat Anti-Mouse IgG (violet), Au NPs and Goat Anti-Mouse IgG (red), fluorophore (green), Au NPs and fluorophore (cyan), Goat Anti-Mouse IgG-fluorophore (magenta), Au NP and Goat Anti-Mouse IgG-fluorophore (black), Mouse Anti-Goat IgG and NPs (yellow) and Mouse Anti-Goat IgG with Au NPs (blue).