Metal-driven Self-Assembly : the Case of Redox-Active Discrete Architectures

The metal-driven self-assembly strategy is well-established for the construction of discrete architectures featuring a cavity. The resulting molecular rings and cages are potentially useful as hosts for complementary guests. Recent years have seen growing interest in the introduction of given functionalities, including redox properties which, among others, allow modulating the ionic charge of the cavity. Depending on which subunit is electroactive, various situations can be encountered, with a global redox activity which is ligand- and/or metal-centered and which involves or not electronic interactions between the constituting units. In this feature article, we propose to survey those different situations by exploring some recent examples of the growing family of redox-active self-assembled rings and cages.


Introduction
The self-assembly methodology, more particularly when assisted by metals, constitutes a very efficient synthetic approach 1 to reach more and more sophisticated macrocyclic structures, otherwise very challenging to prepare through classical step-by-step covalent synthesis.In order to target applications, 2 the resulting discrete structures, be they polygons or cages, may integrate various functionalities.In particular, a strong interest is currently devoted to responsive coordinationdriven self-assembled architectures which can be addressed through external stimuli, as for example irradiation with light. 3n this ground, a growing interest is focused on assemblies responsive to a redox-stimulus and the number of electro-active metalla-rings/cages has been increasing drastically in the very recent years.The use of redox-active precursors along the selfassembly process can in principle produce discrete metallasupramolecular assemblies which are redox-active.Various situations can be encountered, e.g. an electroactivity which is brought either by the organic ligand part (naked or appended with a redox-active unit), by the metal or by a co-ligand.Moreover, through-space or through-bond interactions between the different components can potentially occur.An immediate consequence with such variety of situations lies on the possibility to switch, providing a suitable design, the ionic charge surrounding the cavity, by a simple tuning of the applied redox potential.It is worth noting that in addition to be redoxactive, such systems intrinsically present a cavity which can therefore be used to bind a complementary guest. 4This combination of properties is of particular importance regarding applications for sensing or for controlling encapsulation/ delivery processes of various guests.Finally, the supramolecular assembly approach may constitute a promising future strategy to address the design of organic materials (eg organic photovoltaics and molecular electronic devices).Indeed, mastering the geometry of multicomponents redoxactive systems offers a unique opportunity to fine-tuning electronic interactions within the material, 5 which includes a control over through-space electron hopping or delocalization and which is of prime importance for optimizing electron transport in organic materials.Altogether, the diversity of the redox-active structures which can be attained by the metal-driven self-assembly methodology opens fascinating perspectives for various research topics, ranging from drug delivery, guest sensing, catalysis or even molecular electronics.We propose in this feature article to cover the most recent advances in this field.

2,4,6-tris(4-pyridyl)-1,3,5-triazine-based cages
A prototypal self-assembled host system corresponds to the M 6 L 4 cage 1 (Figure 1a) extensively developed by M. Fujita and Coll., which integrates triazine panels (2,4,6-tris(4pyridyl)-1,3,5-triazine) and square-planar Pd(II) or Pt(II) as metal corners.1u, 6 Because of the three coordinated pyridine moieties, the tridentate triazine core is highly electron-poor.The redox properties of this assembly have been studied in order to address the photoinduced host-guest electron-transfer occurring between panels and alkanes encapsulated within the This journal is © The Royal Society of Chemistry 2012 cavity. 7In particular, this cage is able to form a clathrate complex with four adamantane molecules (1a⊃(adamentane) 4 ).Upon irradiation, the colourless solution of 1a⊃(adamentane) 4  turns blue, indicating the generation of radical species.The photolysis of the complex produces two oxidized derivatives (1-adamantyl peroxide and 1-adamentanol) in a total yield of ∼24% which indicates that only one over the four adamentanes has reacted. 8The proposed reaction mechanism involves the generation of the host anion-radical and guest cation-radical via a guest-to-host photoinduced electron transfer (PET), with only one triazine panel involved.Cyclic voltammetry of cage 1b was investigated and exhibits two redox systems (Figure 1b).A reversible redox wave corresponding to a one-electron process, as confirmed by coulometric experiments and as supported by DFT calculations, is observed at E = -1.02V vs Ag/Ag+, followed by pseudoreversible redox waves from E = -1.40 to -2.00 V, assigned to three electrons in total.It is worth noting that in the case of cages 1a,b, the four triazine panels are not simultaneously reduced which is assigned to an electronic communication occurring between the cage side walls through the Pt ion centers.Such propensity of cage 1b to bind adamantane was very recently explored by J. T. Lippard and coworkers for drug delivery purposes. 9The authors developed a well-defined Pt drug delivery system based on the encapsulation of four adamantyl groups inside the cavity, each of them being appended with a Pt(IV) prodrug unit (AdPt).The cyclic voltammetry of assembly 1b.AdPt 4 in water shows cathodic signals assigned to the triazine core (1b), as well as a less negative one corresponding to the irreversible reduction of Pt(IV).This scheme is confirmed by a chemical reduction of 1b.AdPt 4 with ascorbic acid, which gives rise to a cisplatin release, beside 1-adamantylamine and succinic acid, providing therefore a proof of concept for a controlled delivering of cisplatin through reduction.Finally, one can remind that tris(pyridyl)-triazine cages 1a,b present a good ability to encapsulate a wide variety of organic guests.1u This property was explored in the peculiar case of redox-active guests such as organic radicals of the nitronyl nitroxide type 10 or ferrocene derivatives, 11 whose redox properties are significantly modified upon encapsulation.

Rhenium-based rings and cages
The redox properties of the same tridentate triazine panel, in association with indigo anions and Re(I), was recently studied by C.-Y. Lin, K.-L.Lu and coworkers in the case of the neutral self-assembled triangular metalloprism 2 (Figure 2a), which exhibits strong NIR absorption. 12The presence of electronaccepting indigo and triazine units in the same structure makes this metalloprism highly electron-poor and easy to reduce.Actually, 2 can be electrochemically switched to several polyanionic states through stepwise reduction processes involving up to eight electrons, giving rise to an efficient control over the corresponding optical properties.Four reversible reduction waves are observed from cyclic voltammetry experiments (Figure 2b).Remarkably, this study coupled with EPR analysis and DFT calculations, indicates that the stepwise reduction sequence is essentially ligand-centered.The first redox system at E = -0.29 V vs Ag/AgNO 3 is ascribed to the simultaneous one-electron reduction of three indigo ligands giving rise to 2 3•-, the latter species corresponding to a metal-bound indigo radical anion exhibiting a small d-Reindigo orbital mixing.The second and third reductions (E = -0.73 and -0.98 V) are tentatively assigned to one electron reductions of each triazine ligand, which is supported by EPR  experiments.The splitting of these two reduction waves may be ascribed to a stabilization of a mixed-valence state between the facing tris(pyridyl)triazine units, 13 a behavior which is not observed between the indigo pillars which are distant from each other.The fourth reduction process (E = -1.23 V) corresponds to the second one-step three-electron-reduction process at the indigo-pillared ligands.Interestingly, the metalloprism 2 can be also reversibly oxidized through a three-electron process, corresponding to the oxidation of each indigo dianion.It appears from these data that the structure 2 behaves as an electron reservoir endowed with programmed and well-defined redox-properties.Another rhenium-based family of redox-active polygons corresponds to rectangles 3a-f depicted by J. T. Hupp and coworkers (Figure 3a). 14The latter incorporate four Re(I) corners which are organized in two pairs by doubly chelating planar 2,2'-bisbenzimidazolate ligands and which are bridged by rigid or semirigid ditopic symmetrical (LL) ligands.These systems were designed to study the balance between intramolecular through-space electronic communication (between facing LL ligands) vs coordination-bond-mediated interactions.Spectroelectrochemical studies led on the -1 charge state of the rectangles (i.e.mixed-valence state with respect to the ligands), demonstrated that those systems are dominated by electronic interactions of the former type.A cyclic voltammetry study of rectangles 3a-f was carried out and shows reversible reduction waves that are assigned to stepwise one-electron reductions of the symmetrical LL ligands within the rectangle (for a representative example, see the case of compound 3d, Figure 3b).No oxidative wave is observed and the reduction potentials appear substantially shifted to less negative potentials in comparison with free ligands, which is due to the electrostatic stabilization promoted by the rhenium cations over the negative reduced forms of the ligands.

Perylene bisimide-based rings and cages
F. Würthner and Coll.have developed a series of selfassembled discrete architectures based on the photo-and electro-active perylene bisimide (PBI) unit.A prototypal example corresponds to metalla-square 4, 15 constructed from a tetraaryl-substituted N,N' 4-pyridyl-substituted PBI ligand and square planar Pt(II) complex (Figure 4a).The UV/Vis and fluorescent properties of square 4 are not significantly changed compared to those of the PBI-ligand precursor, which is explained by a lack of electronic communication through the imide substituent.The cyclic voltammetry of square 4 (Figure 4b) reveals two successive reversible reduction waves at E = -1.01 and -1.14 V vs Fc/Fc + , which are slightly shifted compared to the free ligand PBI-ligand precursor (E = -1.08 and -1.23 V respectively).They correspond to the successive radical anion  and dianion formation for each perylene unit, which behave independently contrary to the above-presented triazine-based cages, which is also confirmed by spectroelectrochemical study led on 4 that exhibits redox processes which are exclusively ligand-centered.Finally, a reversible oxidation process is observed for the metalla-square 4 at E = +0.93V vs Fc/Fc + .Therefore, this electrochemical study demonstrate that the ionic state of square 4 can be in principle reversibly tuned from 0 to +12, according three steps of four electrons.Ultimately, this CV study also confirms the electrochemical inertness of the Pt(II) phosphane corner in the E = -1.30V to +0.90V range (vs Fc/Fc + ), this complex being one of the most commonly used for the construction of discrete self-assembled metalla-rings and cages via the coordination-driven approach.As demonstrated by the same group, the PBI panels can also be functionalized with pendant redox-active units such as in metalla-square 5 (Figure 4a) decorated with 16 redox ferrocene moieties. 16onsequently, in addition to show the typical reduction of each PBI unit into the radical anion and dianion states (E = -0.98 and -1.13 V vs Fc/Fc + respectively), compound 5 also exhibits oxidation of the ferrocenyl groups (Figure 4c).Noteworthy, contrary to the reduction of the four PBI panels which occurs simultaneously in 5, the peripheral ferrocene units give rise to a splitting of the ferrocene/ferricinium redox wave, a consequence of through-space interactions occurring between ferrocene units in such a confined space.Though presenting remarkable photophysical and electrochemical characteristics as well as defined cavities, no guest encapsulation from the above-described PBI-based metalla-macrocycles was mentioned by the authors.It is only very recently that the same group designed a robust PBI-based metalla-assembly presenting host-guest properties. 17The very large M 4 L 6 tetrahedron 6 (Figure 5a) is assembled from an octahedral Fe(II) ion and a PBI ligand bearing 2,2'-bipyridine units on the imide positions.The resulting Fe 4 (PBI) 6 tetrahedron exhibits a particularly rich electrochemical activity manifested by multiple redox processes.As seen above, PBI derivatives are electron-accepting moieties that can be successively reduced into radical-anion and dianion species.This is observed in the CV of 6 which exhibits reversible redox waves at E = -0.94 and -1.11V vs Fc/Fc + respectively (Figure 5b).Moreover, assembly 6 incorporates four electroactive Fe(bpy) 3 2+ centers which are known to undergo reversible electrochemical oxidation and reduction processes.The reductions of the bpy units on each metal center are not simultaneous and occur according to three successive oneelectron reduction processes, giving rise to a total of five reversible reduction waves for assembly 6.In addition, two oxidation waves are observed at E = +0.73 and +0.95 V vs Fc/Fc + , assigned to the successive Fe 2+ and PBI oxidations.It is worth noting that tetrahedron 6 is redox-stable for an impressive broad range of charges (from -16 up to +18), which corresponds to a total of 34 electrons which can be switched, simply by controlling the electrochemical potential.The authors attribute this efficient charges balance to the highly porous character of the large tetrahedron 6, which allows a suitable mobility of counterions during the redox switches.Another consequence of the large size of the cavity lies on the ability of 6 for encapsulating C 60 .Moreover, the size complementarity is strengthened by the well-established electronic affinity between C 60 and the PBI moiety.Actually, the authors have shown by ESI-TOF mass spectrometry that not only one, but two fullerene units can be encapsulated within tetrahedron 6, which is one of the largest cavity ever described of the M 4 L 6 family.

Rings and cages incorporating ferrocene subunit(s)
The design and the synthesis of molecular systems constituted from several redox-active units within the same assembly is of attractive interest for various applications such as photochemical devices or information storage.In this context, in addition to the PBI-based assembly 5 described above, several electro-active self-assembled rings and cages decorated with multiple Fc units were reported, notably by the groups of P. J. Stang, H.-B. Yang and coworkers.Using the straightforward coordination-driven self-assembly strategy, the authors were able to build both hexagonal 18 and triangular 19 supramolecular metalla-cycles incorporating ferrocenyl moieties either inside 18a or outside 18b, 18c, 19 the cavity.The general synthetic methodology involves the rational combination of ferrocenyl skeletons presenting 120° (target = hexagons) or 60° (target = triangles) coordination sites, with complementary linear units.For example, the synthesis depicted below (Figure 6a), involves a linear 4,4'-bipyridine ligand with bis-metallic complexes bearing one (target = 8) 18b or two (target = 7) 19 pendant ferrocenyl group(s).For the preparation of hexagons, an alternative approach consists in the combination of two complementary 120° oriented electro-active building blocks (9).18b Electrochemical properties of the resulting multi-ferrocenyl complexes were studied by cyclic voltammetry.All hexagonal assemblies exhibit a single reversible redox wave with several electrons transferred during the oxidation process indicating that all ferrocene units react independently (See example of assemblies 8 and 9, Figures 6c  and 6d).Due to the large cavity size, this phenomenon occurs even when the ferrocenyl units point towards the center of the cavity.18a Remarkably, the redox-active character of the pendant Fc probes provides a unique opportunity to address a quantitative approach which can be supported by electrochemical studies.In particular, the diffusion coefficient (D) of the metalla-cycles could be estimated from the Randles-Sevcik equation by measuring the peak intensity at different scan rates or by potential-step chronoamperometry.The determined D values suitably correlate with the corresponding hydrodynamic diameters of the self-assembled architectures, as expected from the inverse proportionality between D and the metalla-macrocycle size.Therefore, the Fc units offer here an original way to probe electrochemically the size of the edifice, which is in principle complementary to DOSY NMR experiments.Finally, unlike hexagonal assemblies 8 and 9, it has to be noted that in the case of compound 7, 19 the presence of two spatially close Fc units in vicinal positions of each phenanthrene moiety results in a splitting of the associated redox wave in the cyclic voltammogram (Figure 6b).Such behaviour is assigned to a through-space electronic communication between the two proximal Fc units.With the aim of producing supramolecules that combine multiple properties, the same authors recently depicted multifunctional systems associating both an electro-active unit (i.e.Fc) and a crown-ether binding site in a same selfassembled macrocycle (Scheme 1). 20The single redox wave corresponding to the ferrocene unit in compound 10 remains reversible, and allows for determination of the corresponding D values.Its binding properties with a dibenzyl ammonium salt were studied by 1  the dibenzyl ammonium guest, indicating the lack of interaction between pendant Fc and crown-ether subunits.Nevertheless, the voltammogram shape of complex 11 compared to free 10 indicates as expected a lower D value upon complexation.Finally, efforts towards the synthesis of electroactive dendritic metalla-assemblies have to be mentioned. 21Based on a similar exo-functionalization approach as for the metalla-cycles depicted above, the same groups prepared a series of metallodendrimers of controlled size and shape, bearing both Fc and dendrons functional groups.For instance, the reaction between 120° dendritic donors and 120° ferrocenyl acceptors resulted in hexagonal, hexafunctionnalized metallocycles 12a-c (Scheme 2).21d Their cyclic voltammograms reveal a unique redox wave corresponding to the reversible oxidation of the Fc units.Again, the authors could extract the values of the diffusion coefficients D from the CV experiments with, as expected, decreasing values when increasing the molecular weight.A step further lies on the extension of this strategy from metalla-rings bearing Fc units to analogous 3D metalla-cages. 22he cuboctahedral complex 13 (Scheme 3) was obtained from a 120° Fc acceptor complex and a tritopic pyridyl ligand.Its redox properties were explored in detail.Each Fc unit exhibits a similar half-wave potential and no decomposition of the assembly is observed during the redox process.The D values extracted from the electrochemical measurements are similar to those obtained from the DOSY NMR experiment.Remarkably, the corresponding external diameter calculated from the Stokes-Einstein 23 equation (6.7 nm) is close to the value obtained from the molecular force field model structure (6.9 nm).Finally, the diffusion coefficient allows for assessing the presence of twelve redox units on the metalla-cage, indicating that all the Scheme 3 Synthesis of cuboctahedral assembly 13. ferrocenyl moieties are oxidized simultaneously.In addition to these self-assembled rings and cages decorated with pendant redox active Fc units, P. J. Stang and coworkers described a rectangular assembly 14 which incorporates the Fc moiety as a constituting part of the ring skeleton (Figure 7a). 24The electronic properties of 14 were studied by cyclic voltammetry (Figure 7b) and show two reversible waves at E = +0.12 and +0.33 V vs Fc/Fc + .The first redox process was assigned to a two-electron exchange centered on both Fc units and the second one, to the simultaneous oxidation of anthracenyl units.Interestingly, the authors could determine from the spectroelectrochemical analysis that the intermediate 14 2+ coexists in equilibrium with the neutral and the tetracationic forms.

Rings and Cages incorporating the tetrathiafulvalene core
The tetrathiafulvalene (TTF) moiety has received a great deal of attention as a key building block in the preparation of conducting and semiconducting materials. 25Its remarkable electrochemical behaviour is characterized by two reversible oxidation steps at low potentials, which produce stable oxidized species TTF •+ and TTF 2+ .This property has promoted this unit a key building block for the elaboration of numerous molecular and supramolecular switchable systems. 26Beside to offer an extra redox state compared to ferrocene, another key issue of TTF lies on its planar -conjugated geometry.Therefore, unlike ferrocene, TTF and its derivatives can constitute themselves the side walls of the cavity upon a self-assembly process, giving rise to metalla-rings or -cages endowed with electron rich internal cavities.J-L.Zuo and coworkers described neutral complexes 15 and 16 (Figure 8a). 27The latter was obtained in one step by the reaction of a mixture of cis and trans bis(pyrazole)-TTF ligands with Re(CO) 5 Cl.Their XRD analysis revealed that the TTF skeleton is bended in 15 and planar in metalla-ring 16.The high geometric constraints occurring in such small size selfassembled systems, has of course a strong impact on the electronic integrity of the TTF unit (Figure 8b).In both cases, the usual well-defined two-steps redox behaviour of TTF cannot be recovered, due to the strong geometric constraint applied to the TTF core upon oxidation in the case of 15 and to an electronic coupling between the facing TTF redox units in the case of 16.
Another metalla-ring involving a metal carbonyl complex and a TTF derivative, namely vinylogous tetrathiafulvalene (TTFV), was described by D. Lorcy and coworkers (Figure 8c). 28The latter is constructed from the self-assembly of a non-planar bispyridyl TTFV ligand with hexacarbonyl molybdenum in a 1:1 molar ratio.The electrochemical properties of metallasquare 17 which incorporates four redox-active units, were studied by cyclic voltammetry.The system appears to be oxidized according to two successive processes at E = +0.48 and +0.74 V vs SCE.The first one is attributed to the 0/•+ oxidation of the organic part, which is slightly shifted compared to the TTFV ligand precursor, the second wave being ascribed to the superimposition of both metal centers oxidation with the •+/2+ ligand centered oxidation.In the course of the studies led at Angers, related to the synthesis of electron-rich supramolecular discrete systems, we described recently several examples of metalla-rings and -cages whose cavities are made from TTF or its derivatives.For this purpose, several di-and tetra-topic ligands either designed from the parent TTF skeleton (18) or two of its well-known derivatives, namely bis(pyrollo)TTF (BPTTF) 29  (19, 20) and the so-called extended-TTF (exTTF) 30  (21) were synthesized (Scheme 4).As a common feature, all of these ligands integrate a central sulfur-rich electron-rich framework.A first example of a metaldriven self-assembled system issued from the parent TTF, corresponds to the octanuclear metalla-cage 22 which was prepared in collaboration with B. Therrien and Coll.(Figure 9a).Reaction between tetratopic ligand 18 and dinuclear pcymene oxalato ruthenium acceptor Ru1 afforded metalla-cube 22, whereas the reaction led with dinuclear p-cymene quinonato complex Ru2 afforded tetranuclear plate 23. 31 The selective formation of these two different architectures results from geometrical complementary interactions between 18 and the respective Ru1 and Ru2 counterparts.Two pyridyl nitrogen atoms located on the same dithiol unit in ligand 18 are separated by 6.7 Å, whereas both ruthenium centers are distant of 5.5 Å in complex Ru1 and of 7.9 Å in complex Ru2.Therefore, the good size matching between ligand 18 and Ru2 .This journal is © The Royal Society of Chemistry 2012 .
affords tetranuclear plate 23 whereas the smaller ruthenium acceptor Ru1 generates cage 22.Both assemblies were studied by cyclic voltammetry (Figure 9b).Two characteristic redox waves corresponding to the TTF moiety are observed in plate 23 (E = +0.28 and +0.58 V vs Fc/Fc + ), shifted to higher potential in comparison to ligand 18 (E = +0.22 and +0.55 V vs Fc/Fc + ) as a consequence of the coordination to the metal centers.The electrochemical behaviour observed for metallacage 22 is significantly different since only one oxidation wave is observed, at E = +0.44V vs Fc/Fc + .This peculiar behaviour is assigned to the rigidity of the metalla-assembly which may alter the kinetics associated to the conformational changes which usually occur upon electrochemical oxidation of TTF derivatives.It is indeed well-established that the TTF parent system undergoes severe conformational changes upon oxidation to heteroaromatic 1,3-dithiolium cations TTF .+ and TTF 2+ .Whereas neutral TTF corresponds to a distorded boatlike structure, TTF .+ is planar and TTF 2+ is significantly twisted around the central C-C bond.25a, 32 Consequently, if structural elements located on the periphery of the TTF skeleton prevent those conformational changes upon oxidation, the usual welldefined reversible two-steps redox behaviour of TTF is not observed anymore.This is observed for instance with geometrically constrained tetrathiafulvalenophanes 33 or TTFbased cages molecules, 34 and is also observed in the case of metalla-cage 22.Another family of electro-active discrete selfassembled cages could be synthesized from ligand 18. Reaction of the latter with cis-blocked Pd(II) or Pt(II) complexes (i.e.cis-M(dppf)(OTf) 2 ; dppf = 1,1′-bis(diphenylphosphino)ferrocene; OTf = trifluoromethane-sulfonate) in a 1:2 molar ratio afforded metalla-architectures 24a (Pt) or 24b (Pd). 35Both present estimated hydrodynamic radii of ca.16 Å as extracted from DOSY NMR experiments which, in addition to other spectroscopic data, well agrees with a M 8 L 4 cubic geometry (Figure 10a).Cyclic voltammetry experiments led on metallacages 24a and 24b show three reversible redox processes.Two are assigned to the TTF side walls and the third one to the corner ferrocene units.Interestingly, the latter can be used as an internal reference to address the number of electrons exchanged.The relative peak intensities of the three systems (i.e.1/1/2) observed from the deconvoluted cyclic voltammogram (Figure 10b) agree with two successive independent oxidations of the four TTF units at E = +0.30and +0.60 V and of the eight ferrocenes at E = +0.80V vs Fc/Fc + , giving rise in total, to the reversible generation of sixteen positive charges on the periphery of the cavity.Molecular squares prepared by the coordination-driven self-assembly strategy are typically issued from the interaction between a linear ditopic ligand and a cis-blocked square planar metallic center.Depending on the length of the linear ligand or on its flexibility, the reaction can also lead to triangular systems beside the molecular square, both polygons being usually difficult to separate.Thus, reaction of the linear N,N'dipyridyl(BPTTF) ligand 36 19 with an equimolar ratio of cis-Pt(dppp)(OTf) 2 , produces a mixture of triangle 25 and square 26 (Figure 11a).Remarkably, in this case, each polygon could be easily isolated by selective precipitation. 37Both metallarings 25 and 26 were fully characterized by different methods.Noteworthy, the ratio between their respective experimental radii (R triangle /R square ) extracted from DOSY NMR reaches a value of 0.80, in excellent agreement with the theoretical value.
As usually observed for BPTTF derivatives, triangle 25 and square 26 exhibit two reversible oxidation waves (E = +0.70 and +1.00 V vs Fc/Fc + ) (Figure 11b).In order to address the number of electrons exchanged in each process, ferrocene was added to the electrochemical solutions and used as an internal coulometric reference (i.e. three equivalents for triangle 25 and four equivalents for square 26).Analysis of the resulting respective peak intensities from the deconvoluted CV (Figure 11b), suggests that the BPTTF side walls behave independently and that the metalla-rings are fully oxidized to their respective 25 6+ and 26 8+ stable states.Given the -donating character of BPTTF derivatives, a peculiar feature of such polygons lies on the strong electronic density which is spread over the walls of the cavity, which in principle is favourable to bind a complementary electron-poor guest within the cavity.On the other hand, the preparation of molecular and supramolecular hosts able to bind fullerene derivatives is a subject of intense interest. 38In this context, the binding affinity of 25 and 26 for electro-deficient C 60 or C 70 was monitored by UV-Vis titrations.The titration spectra as well as a Job's plot analysis (Figure 11c) demonstrate a good binding affinity of triangle 25 for C 60 (or C 70 ) in a 1:1 stoichiometry.This result is supported by a good electronic complementarity of both components as well as by a suitable size matching between the cavity of 25 and a fullerene guest.The latter property is not satisfied in the case of square 26 whose cavity is much larger and which indeed does not exhibit any affinity for those fullerenes upon UV-vis titration experiments.
With the aim to switch from electron-rich metalla-rings to metalla-cages, the tetratopic BPTTF-based ligand 20 (Scheme 4) was designed.The latter was reacted with square planar cis-Pt(PEt 3 ) 2 (OTf) 2 complex and the reaction converged in less than two hours into a single discrete prismatic cage 27 (Figure 12a). 39The M 6 L 3 formula was confirmed by ESI-MS mass spectroscopy and by an X-ray diffraction experiment (Figure 12b).Cyclic voltammetry of metalla-cage 27 (Figure 12c) reveals the presence of two successive oxidation waves, located at fairly low potentials (E = +0.43 and +0.62 V vs Fc/Fc + ) but poorly reversible due to a combination of steric and electronic factors.The guest binding capability of prism 27 was studied by UV-Vis spectroscopy with an electro-deficient compound (TCNQ-F 4 ).The progressive addition of the guest to a solution of electron-rich M 6 L 3 cage 27 leads to the formation of an inclusion complex with a 1:1 stoichiometry as extracted from a Job's plot analysis.A third family of discrete metalla-assemblies that we have been developing 40 is based on the exTTF framework.This donating skeleton exhibits unique electronic and geometrical properties that have been widely explored by N. Martín and coworkers in the construction of covalent hosts prone to encapsulate electro-deficient fullerene guests.38a On this basis, tetrapyridyl-exTTF ligand 21 (Scheme 4) was synthesized in one step from pristine exTTF through a palladium catalyzed C-H arylation.A mixture of ligand 21 and cis-Pd(dppf)(OTf) 2 in a 1:2 molar ratio afforded in only five minutes one discrete assembly 28a (Figure 13a). 40Both species, ligand 21 and resulting M 4 L 2 assembly 28a (Figure 13b) could be characterized by X-ray diffraction.Ligand 21 displays the usual exTTF butterfly shape with a 86° dihedral angle between both 1,3-dithiol-2-ylidene mean plans.This curvature is amplified in the M 4 L 2 container 28a with a dihedral angle of 56° which gives rise to an ovoid cavity whose size and shape are suitable for inclusion of a planar guest.In particular, the formation of a 1:1 host-guest inclusion complex with perylene (Ka ≈ 3.9 x 10 3 ) could be demonstrated by 1 H NMR, DOSY NMR and ESI-FTICR.Electrochemical properties of ligand 21 and container 28a were studied by cyclic voltammetry (Figure 13c).In addition to a first oxidation wave attributed to the twoelectrons oxidation steps of each exTTF scaffold (E = +0.57V vs Fc/Fc + ), container 28a exhibits a second oxidation process centered on the four ferrocene units (E = +0.80V).Compared to ligand 21, oxidation of exTTF in the complex is shifted to higher potentials due to the This journal is © The Royal Society of Chemistry 2012 coordination to the metal center and appears irreversible presumably because of the rigidity of the self-assembly which prevents redox-driven conformational changes which usually occur with exTTF derivatives.

Miscellaneous
Self-assembled polygons/polyhedra are prepared from complementary ligands and metal complexes and the redox activity of the resulting assemblies is closely linked to the electroactivity of each partner.Basically, the redox character of the metalla-ring/cage can be mainly localized on the cavity walls (in general the ligand) as shown above through various examples, or on the corner (in general the metal), or on both partners.In the latter case, the ligand and the metal can behave independently or are sometimes electronically coupled as shown through some examples above.
A promising development of self-assembled metalla-cages concerns their use as anti-cancer agents.1a, 1t, 41 Based on the fact that various Ru(III) and arene Ru(II) complexes exhibit interesting cytotoxic effects, B. Therrien and Coll.have developed several families of water soluble cationic metallacages constructed from arene ruthenium complexes.1t, 41a, 41c The authors have demonstrated the efficiency of such assemblies for increasing the solubility and cell permeability of drug-like molecules or photodynamic therapy (PDT) agents.
Some of these metalla-assemblies were electrochemically studied.An illustrative example is given with the tetranuclear metalla-rectangle 29 (Figure 14a), 42 which is obtained from the reaction of a bipyridine ligand with a dinuclear arene ruthenium complex for which two Ru centers are bridged by a 1,4benzoquinonato moiety.The redox properties of metallarectangle 29 have been studied by cyclic voltammetry (Figure 14b).Compared to the dinuclear arene ruthenium precursor Ru2, rectangle 29 appears much easier to reduce, due to the presence of four positive charges in the metalla-assembly.Rectangle 29 undergoes three successive reduction waves at E = -0.68(reversible, vs Fc/Fc + ), -1.39 (irr.) and -1.90 V (irr.) and no oxidation could be detected before electrolyte decomposition.Though the ligand orbitals are closely mixed with metal-based ones, these reduction processes can formally be assigned to electrons injection occurring predominantly onto the quinonato bridging ligand. .
A similar electrochemical behaviour (Figure 14c) could be observed by P. Mukherjee and Coll., from a 3D macrocyclic cage 30 (Figure 14a) featuring the same bis-bidentate quinonato Ru complex Ru2, which is associated in this case with a tetraimidazole ligand. 43Note that 2D analogous assemblies based on bis-imidazole ligands were also electrochemically characterized. 44Finally, the highly modular character of the stepwise strategy using half-sandwich piano-stool complexes for the construction of discrete electroactive polygons, is illustrated by the possibility to change either the bridging dipyridyl ligand to a longer one as in rectangle 31, 45 or the metal as in 32 which integrates an iridium metal complex instead of a ruthenium one.1q, 46 Beyond Ru-based cages and as mentioned above with the encapsulation of a Pt(IV) prodrug AdPt, 9 metalla-cages incorporating square planar Pt(II) and Pd(II) complexes have also been used as drug delivery vectors.This is the case with metalla-cages 33a-d recently described by J. D. Crowley and Coll.(Scheme 5a) which integrate a variety of functional groups on the outer surface of the host cage.They were prepared according a very efficient modular approach based on the CuAAC 'click' reaction. 47Using this strategy, various functions were appended on the periphery of the cage, some being redox-active.The cyclic voltammetry study of compounds 33a-d in DMF shows basically a superposition of the redox contribution of each electroactive partner, be they reducible or oxidable.They display irreversible reductions at negative potentials (ca.E = -1.7 and -1.9 V vs Fc*/Fc*+) assigned to the 2,6-bis(ethynyl)pyridine ligand framework as well as redox processes corresponding to the appended functional groups.In particular, the ferrocenyl-substituted cage 33a displays the usual reversible oxidation process of ferrocene at E = +0.5 V vs Fc*/Fc*+ and no electronic communication was observed between the four pendant Fc units, as expected from their large spatial separation.With a similar objective to decorate the outer surface of a metalla-structure, R. Yang et al. described the M 12 L 24 sphere 34 (Scheme 5b) which integrates 24 thiophene units. 48nvestigation of the electrochemical properties of 34 in DMSO is characterized by a reduction process at E = -1.06V vs Ag/AgCl.No information is provided regarding the oxidation and electropolymerization ability from this thiophene-rich system.
The case of square 35 (Scheme 6) depicted by A. J. Lees and Coll. 49is interesting since it encompasses three redox active centers, i.e.Ru(II)/Ru(III), Fc/Fc + and the terpyridine ligand, all of them behaving independently.Two oxidation waves are observed and assigned to the Ru(II)/Ru(III) (E = +0.70V vs Fc/Fc + ) and to the Fc corner (E = +0.88V) respectively, whereas reduction of the terpyridine fragment gives rise to two successive reduction processes.The comparison of the electrochemical behaviour of cyclen Ru-based triangle 36 and square 37 (Figure 15a) described by J. R. Long and coworkers, provides an interesting case for using the electrochemistry tool in probing a molecular structure. 50  (cyclen) 4 Ru 4 -(4,4'-bpy) 4 ](OTf) 8 37.The former is oxidized following three distinct redox processes at E = +0.23,+0.37 and +0.48 V vs Fc/Fc + in acetonitrile, each of them corresponding to a Ru-centered one-electron exchange, whereas square 37 exhibits one single reversible four-electron process at E = +0.48Vand high scan rate (1000 mV.s -1 ).This difference is assigned to the more sterically constrained structure in the case of the triangle, which prevents any rotation of the pyridine rings with respect to each other and which favours electronic coupling by delocalization.Such a difference between homologous polygons nicely illustrates how the geometry of molecules built from the same components, can have a direct impact on their electrochemical properties.Because of their unusual topology and as illustrated with 36, metalla-triangles are appealing for the study of their redox properties.Another relevant example corresponds to Ru-based triangles 38a,b built from adenine ligands (Figure 16).These metalla-rings, depicted by J. A. Thomas and coworkers, 51 display a well-defined reversible electrochemical behaviour which is manifested by three Ru-based reversible oxidation processes, from which the authors could demonstrate occurrence of two discernible mixed-valence states.
The case of the phenothiazine heterocycle is interesting since this system can be reversibly oxidized into a radical-cation at E = +0.73V vs SCE (acetonitrile) with the possibility of further oxidation or disproportionation.This favourable redox behaviour prompted G. H. Clever and Coll. to describe recently a phenothiazine-based coordination cage 39a.1c, 52 The latter corresponds to an interpenetrated double cage based on eight phenothiazine ligands and four square-planar-coordinated Pd(II) cations (Figure 17).Remarkably, the corresponding Smonooxygenated sulfoxyde and S-dioxygenated sulfone cages 39b and 39c could be successively obtained from 39a through a stepwise chemical oxidation with Cu(NO 3 ) 2 .3H 2 O or, in the case of 39b, upon standing in air for two months.3] Interestingly, titration with the oxidant[Fe(III)(bipyridine) 3 ] 3+ followed by reaction with water, shows that the intermediate cation radical 39a 8•+ reacts much faster with water compared to the cation-radical generated from the phenothiazine ligand precursor.This is ascribed to a cage effect which, upon oxidation, favours an intramolecular  disproportionation.This property results in neutral and dicationic phenothiazine facets in the double cage, the latter dications being known intermediates in the production of Smonooxygenated sulfoxyde upon reaction with water.Finally, an interesting case concerns metalla-rectangle 40 described by C. N. Hsu and Coll.. 54 This tetranuclear iron thiolate complex can be electrochemically reduced according three successive reversible redox processes (E = -0.28,-0.84 and -0.93 V vs Fc/Fc + ) (Figure 18).It appears that in this rectangle, the 1,4diisocyanobenzene ligand does not assist the electron communication between both iron thiolate cores, which behave independently at the first two-electron redox process (40 4+ /40 2+ ).The second and third processes are assigned to two one-electron exchanges corresponding to 40 2+ /40 + and 40 + /40 0 couples respectively, redox states for which a weak exchange interaction seems to occur through the 1,4-diisocyanobenzene ligand.The most striking feature of this system, which could be demonstrated thanks to the single-crystal X-ray structures of 40 4+ and 40 2+ , comes from their different cavity size, which appears larger in the tetracationic form than in the reduced one.Therefore, this work provides a nice illustration of how the size of a self-assembled redox-active system can be controlled thanks to a redox stimulus, opening therefore interesting perspectives in terms of electrochemical-triggering of guest binding.

Conclusions and Outlooks
Thanks to the synthetic efficiency and to the modular character of the self-assembly approach, a wide diversity of discrete redox-rings/cages have been made available during the last decade.This diversity is supported by the cumulated inherent structural and electronic characteristics of the starting building blocks, i.e. ligands and metal complexes or can also results from synergetic effects between both components.The latter can be electronically coupled or not, giving rise to a large panoply of reducible or oxidable discrete cavities which differ by the structure or by the function.From this point of view, and as shown through various examples in this article, a key issue of such redox-active systems relies on the possibility to fine tuning the charge state of the cavity, be it positive or negative, by a simple control over the applied electrical potential or of the oxidizing/reducing chemical agent.On this ground, it is worth mentioning that beyond potential applications in electrochemical sensing for environmental and medical sciences 4,55 or for redox catalysis, 1i a valuable extension of such control over the cavity charge lies on the possibility to trigger: i) the cavity size, as in the case of metalla-rectangle 40

54
, ii) the binding affinity between the redox-active host cavity and a given guest, a key issue for delivery applications.From this point of view, the possibility to trigger the host-guest affinity thanks to an external physical stimulus is the subject of an intense interest.Promising results have been already obtained from discrete self-assembled host cavities, as for example in the case of light-switchable metalla-cages.1c, 3 Chemical stimuli such as addition of a competing ligand or the use of pH-sensitive components 56 have also been used successfully.Regarding redox-stimuli, being able to tune the charged state of the host cavity -that is, its ionic levelprovides a strong interest.Such system should allow in principle to bind, or conversely to expel the guest, simply by modifying electrostatic host-guest interactions.This concept has already been demonstrated for instance with a molecular organocyanometalate box which permits a redox-switch of the complexation/decomplexation process of K + and Cs + . 57Given the tremendous advances recently achieved regarding the design of new highly functionalized redox-responsive selfassembled hosts, promising perspectives are therefore offered for extending this concept to the binding control of various guests of interest, be they ionic or neutral.
Unlike triangle 25 and square 26 presented above and which exhibit similar cyclic voltammograms, quite different ones are observed for the triangular (cyclen) 3 Ru 3 (4,4'-bpy) 3 ](PF 6 ) 6 36 and the square This journal is © The Royal Society of Chemistry 2012
This journal is © The Royal Society of Chemistry 2012 Dr Sébastien Goeb was born in Schiltigheim (France) in 1979.He studied chemistry at the University of Strasbourg and obtained his PhD degree in Organic Chemistry in 2006 under the supervision of Drs Raymond Ziessel and Antoinette De Nicola.He then carried out two successive one year postdoctoral stays respectively with Prof. Felix N. Castellano at the University of Bowling Green (USA) and with Dr Jean-Luc Parrain at the Institut des Sciences Moléculaires de Marseille (France).He became Chargé de Recherche CNRS in the laboratory MOLTECH-Anjou in 2008.His research interests focus on organic chemistry of photo-and/or redox-active supramolecular discrete architectures.Prof. Marc Sallé was born in Toulon (France) in 1963.He studied chemistry at the Ecole Nationale Supérieure de Chimie de Rennes (ENSCR) and at the University of Paris VI.He received his PhD degree in Organic Chemistry from the University of Angers in 1991 under the supervision of Professor Alain Gorgues.For this work he was awarded the Dina Surdin prize of year 1992.After a postdoctoral stay with Prof. Martin R. Bryce at the University of Durham (UK), he became Maître de Conférences at the University of Angers, where he was promoted to Prof. in 1998.He has been a junior member of the Institut Universitaire de France (IUF).His research interests concern organic chemistry of molecular materials and supramolecular chemistry associated with electro(photo)-active molecular systems.Prof. Marc Sallé is currently the director of the Laboratory MOLTECH-Anjou (Angers, France).