Self-assembled supramolecular cages containing ruthenium(ii) polypyridyl complexes

Ruthenium(II) polypyridyl complexes exhibit remarkable photophysical properties and stability, continuing to find new applications including water splitting, visible light photoredox processes and biological imaging. Many examples of ruthenium(II) polypyridines have been reported being incorporated into extended structures such as organic polymers, vesicles, coordination polymers and networks, as well as acting as photosensitizers for molecular machines and as components of discrete 2D macrocycles or related assemblies. However, examples of 3D molecular cages containing Ru(II) centres remain rare, in part due to the inert nature of this low-spin d complexes. Herein we report, to the best of our knowledge, the first structural characterisation of incorporation of the prototypical [Ru(tpy)2] 2+ (tpy = 2,20,60,200-terpyridine) unit into a discrete 3D molecular cage. Functionalised [Ru(tpy)2] 2+ units are particularly appealing as only a single isomer of this complex is possible, in contrast to bidentate analogues such as Ru(bpy)3 2+ (bpy = 2,20bipyridine), which greatly simplifies the number of possible species formed when linking multiple Ru(II) centres. Complexes [Ru(1)2] 2+

Ruthenium(II) polypyridyl complexes exhibit remarkable photophysical properties and stability, 1 continuing to find new applications including water splitting, 2 visible light photoredox processes 3 and biological imaging. 4Many examples of ruthenium(II) polypyridines have been reported being incorporated into extended structures such as organic polymers, 5 vesicles, 6 coordination polymers 7 and networks, 8 as well as acting as photosensitizers for molecular machines 9 and as components of discrete 2D macrocycles 10 or related assemblies. 11However, examples of 3D molecular cages [12][13][14] containing Ru(II) centres remain rare, 12,15 in part due to the inert nature of this low-spin d 6 complexes.Herein we report, to the best of our knowledge, the first structural characterisation of incorporation of the prototypical [Ru(tpy) 2 ] 2+ (tpy = 2,2 0 ,6 0 ,2 00 -terpyridine [16][17][18] ) unit into a discrete 3D molecular cage. 19Functionalised [Ru(tpy) 2 ] 2+ units are particularly appealing as only a single isomer of this complex is possible, in contrast to bidentate analogues such as Ru(bpy) 3 2+ (bpy = 2,2 0bipyridine), which greatly simplifies the number of possible species formed when linking multiple Ru(II) centres.Complexes [Ru(1) 2 ] 2+ and [Ru(2) 2 ] 2+ feature inert [Ru(tpy) 2 ] 2+ units decorated with pendant pyridyl groups capable of binding additional metal centres (Fig. 1). 20quare planar Pd(II) complexes are well suited to binding pyridyl ligands to form metallosupramolecular structures, using approaches pioneered by Fujita 21 and Stang. 22Reaction of [Ru(1) 2 ](PF 6 ) 2 with two equivalents of Pd(dppp)(OTf) 2 (dppp = 1,3-diphenylphosphinopropane) in nitromethane at room temperature (Fig. 2) immediately gave a single major species in solution, as characterised by 1 H, 13 C and 31 P NMR (Fig. 3).This assembly appears quantitative by NMR.The four-fold symmetry of the parent complex (Fig. 3a) is maintained in the product indicating the formation of a highly symmetric species with all tpy ligands being equivalent.Significant changes in NMR chemical shifts and very considerable broadening of signals at room temperature are observed upon Pd(II) coordination.Variable temperature NMR confirmed room temperature to be close to the coalescence temperature (in CD 3 NO 2 or CD 3 CN).High temperature (348 K, Fig. 3c) 1 H and heteronuclear 2D NMR experiments (see ESI, † Fig. S13-S17 for details) were used to unambiguously assign all proton and carbon NMR signals.The signals of the protons of the pendant pyridyl groups are shifted slightly upfield (Dd H D2 À 0.09; H D3 À 0.17 ppm; Fig. 3a  and b) upon coordination to the Pd(II) centre.However, the most significant changes in chemical shifts are for the signals of the terpyridine moiety, which is distant from the Pd(II) coordination site.Signals corresponding to the protons of the terminal pyridine ring (ring A, see Fig. 1 for labelling scheme) are shifted upfield (Dd ppm: H A3 À 0.46; H A4 À 1.03; H A5 À 1.34; H A6 À 0.54) upon coordination with the Pd(II) corner units.These upfield shifts are consistent with the highly shielding environment expected within a molecular cage. 23The Electrospray ionisation mass spectrometry (ESI-MS) of a nitromethane solution of the product resulted in the formation of a relatively abundant distribution of ions that were charged from +5 to +10 (Fig. 4).The difference in m/z values between adjacent ions in the distribution and the isotopic patterns were assigned to [3(PF 6 À ) 24Àn ] n+ (n = 5 to 10), formed by the sequential loss of PF 6 counter anions from [3(PF 6 ) 24 ] (11 742 Da; Fig. 4).This Ru 4 Pd 8 tetrameric cage structure, [3](PF 6 ) 24 , is the smallest least-strained structure possible for this system. 24Additional peaks were observed corresponding to the loss of one Pd(dppp) 2+ unit which were confirmed by collision-induced dissociation (CID) experiments.This structure is also consistent with the broad 1 H NMR signals observed in solution, as the {Ru(tpy) 2 } units rotate slowly on the NMR timescale and the environment inside and outside of the cage is non-equivalent.This restricted rotation is due to the close contact between the {Ru(tpy) 2 } groups forming something resembling a poorly assembled gear box (see later X-ray structure discussion).
Although stable in acetonitrile at high concentrations (41 mM), based on 1 H NMR, the cage disassembles upon dissolution in acetonitrile (see Fig. S25, ESI †), which is not surprising given this solvent can act as an excellent ligand for Pd(II) centres.However, the complex was observed to be stable in pure nitromethane (Fig. S26, ESI †), a polar but very weakly coordinating solvent, over the same   concentration range, confirming this solvent as an excellent choice for this class of metallosupramolecular systems.The analogous reaction with complex [Ru(2) 2 ](PF 6 ) 2 , which features alkyne spacers between the phenyl and pendant pyridyl rings, and Pd(dppp)(OTf) 2 also formed a single major product in solution (Fig. 5).The 31 P{ 1 H} spectrum (Fig. S29, ESI †) again revealed a single sharp singlet at 8.91 ppm, effectively identical to that observed for [3] 24+ , and consistent with the formation of a single major product.However, in contrast to cage 3 24+ , the 1 H NMR signals (Fig. 3e) of this new species were not significantly broader than the parent Ru(2) 2+ complex, suggesting the rotation of {Ru(tpy) 2 } units was effectively unhindered in this structure.The 1 H NMR signals corresponding to the pendant pyridine protons were shifted relative to [Ru(2) 2 ](PF 6 ) 2 (Dd H D2 + 0.05; H D3 À 0.32), but as was the case for cage 3 24+ , the signals of the terpyridine group were significantly affected, all being shifted upfield (Dd H B3 À 0.31; H A3 À 0.19; H A4 À 0.26; H A5 À 0.24; H A6 À 0.28) but to a much less extent than in cage 3 24+ .These peak shifts correspond to a cage environment considerably less shielded than in cage 3 24+ .The 13 C{ 1 H} NMR spectrum (Fig. S28, ESI †) reveals relatively small changes in peak shifts for most signals, with the notable exceptions of the pendant pyridyl signals (Dd C D2 À 0.3; C D3 + 2.7; C D4 + 3.6 ppm), the alkynes (Dd C C -CRC + 4.5 ppm; C D -CRC À 1.5 ppm) and the central phenyl ring (Dd C C1 À 1.6; C C2 + 0.2; C C4 + 2.0; C C5 + 0.1 ppm).These peak shifts reflect not only the electronic effect of Pd(II) coordination to the pyridyl group, but also the strain introduced to the alkyne upon bending to form a smaller cyclic structure, hence the observed changes are significantly different to that observed for cage 3 24+ .
The ESI-MS of a solution of this product (Fig. S36, ESI †) revealed a trimeric, rather than tetrameric structure, consistent with the structure of cage 4(PF 6 ) 18 (Fig. 5) indicating the additional flexibility of the alkyne spacers was sufficient to allow a smaller structure to form and simple molecular modelling (MMFF, Fig. S40, ESI †) supports this assignment.This structure was found to precipitate from solution (acetonitrile) over time to form an insoluble red powder, presumably a coordination polymer.In nitromethane the cage appears stable over several months in solution.
Slow diffusion of toluene into a nitromethane solution of cage 3(PF 6 ) 24 gave red block crystals suitable for X-ray diffraction.‡ The molecule crystallises in the P% 1 space group with the asymmetric unit containing half of one cage molecule and disordered solvents and anions (Fig. 6).The complex forms a box-like structure approximately 21 Â 21 Â 32 Å in dimensions with Pd(II) centres at each end forming near perfect squares (Pd-Pd-Pd angles of 86.0-92.81 and PdÁ Á ÁPd distances of 13.2-13.4Å).The centre of the cage is occupied by {Ru(tpy) 2 } units with alternating RuÁ Á ÁRu distances of 11.82 and 8.78 Å and inter-{Ru(tpy) 2 } pyridineÁ Á Ápyridine separations of (centroidÁ Á Á centroid) 3.86 and 5.34 Å forming portals which are occupied by PF 6 À counterions.The cavities and each end of the cage are sufficiently large to potentially accommodate large guests such as a C 60 molecule.The {Ru(tpy) 2 } groups form pairs of terpyridine 'embraces' 25 (Fig. S37, ESI †), a type of favourable edge-to-face and face-to-face aromatic interactions, as often seen in solid state packing of simple {M(tpy) 2 } n+ complexes.These interactions reveal the origin of the restricted rotation of these units in solution.Although not requiring a concerted rotation of all the {Ru(tpy) 2 } units, it appears this type of  This journal is © The Royal Society of Chemistry 2015 favourable p-p stacking interactions are more significant than simply steric crowding and result in the hindered rotation of these units.The cages are assembled together in the crystal structure via additional intermolecular terpyridine embraces to form 1D chain along the crystallographic a axis as well as extensive p-p interactions between the pyridine and phenyl rings along the b axis (Fig. S39, ESI †).Preliminary investigations of the photophysical properties of cage [3](PF 6 ) 24 indicate the functionality of the parent complex is retained in the cage structure.The 1 MLCT absorption maxima of the [Ru(1) 2 ](PF 6 ) 2 20 complex and cage [3](PF 6 ) 24 were both located at 490 nm, while the 3 MLCT emission spectra were essentially superimposable centred at 640 nm.The excited state lifetimes (1.26 AE 0.01 ns and 1.21 AE 0.01 ns respectively) were similarly identical, and are comparable to those of related [Ru(4 0 -tolyltpy)(tpytpy)] 2+ complexes (see ESI † for details). 26hree dimensional molecular cages containing [Ru(tpy) 2 ] 2+ units are reported and characterised in solution and the solid state.The photophysical properties of the parent Ru(II) complex [Ru(1) 2 ] 2+ are retained in the cage 3 24+ , suggesting this new class of molecular cages may be potential candidates to act as photosensitizers for bound guest molecules.The introduction of an alkyne spacer, producing a larger ligand, resulted in the formation of a smaller, trimeric cage highlighting the flexibility of these spacer units and the subtlety of the assembly process.

Fig. 1
Fig.1Structure of complexes that are building blocks of more complex supramolecular cages, showing the numbering scheme adopted.
This work was supported by a National Science Foundation of China (NSFC) Research Fund for International Young Scientists Project (No. 21450110060 and 21271102).The Australian Research Council is acknowledged for Future Fellowship (EGM, FT100100795) and Discovery Early Career Research Awards (WAD, DE130100424).Photophysical measurements were undertaken at the Photochemistry and Ultrafast Laser Spectroscopy (PULS ) facility, School of Chemistry and Molecular Biosciences, with financial support from the University of Queensland (MEI-2013000106).Crystallographic data was collected in-house at UNSW, or at the MX1 beamline at the Australian Synchrotron under a Collaborative Access Program (AS143_MXCAP_8503).