An overview of recent developments in the analytical detection of new psychoactive substances (NPSs)

New psychoactive substances (NPSs), sometimes referred to as “ legal highs ” in more colloquial environments/the media, are a class of compounds that have been recently made available for abuse (not necessarily recently discovered) which provide similar e ﬀ ects to the traditional well studied illegal drugs but are not always controlled under existing local, regional or international drug legislation. Following an unprecedented increase in the number of NPSs in the last 5 years (with 101 substances discovered for the ﬁ rst time in 2014 alone) its, occasionally fatal, consequences have been extensively reported in the media. Such NPSs are typically marketed as ‘ not for human consumption ’ and are instead labelled and sold as plant food, bath salts as well as a whole host of other equally nondescript aliases in order to bypass legislative controls. NPSs are a new multi-disciplinary research ﬁ eld with the main emphasis in terms of forensic identi ﬁ cation due to their adverse health e ﬀ ects, which can range from minimal to life threatening and even fatalities. In this mini-review we overview this recent emerging research area of NPSs and the analytical approaches reported to provide detection strategies as well as detailing recent reports towards providing point-of-care/in-the-ﬁ eld NPS ( “ legal high ” ) sensors.


Introduction
New Psychoactive Substance (NPS) is an umbrella term to refer to substances which mimic the effects of common illicit materials (for example, methamphetamine and cannabis) however they are not controlled by drug legislation such as the Misuse of Drugs Act 1 in the United Kingdom and other similar controls internationally.Designed, in some cases deliberately, to evade international control, NPSs may pose a significant danger to the health and safety of the public.As with controlled substances, NPSs are understood to have potentially negative short-term side effects such as paranoia, psychosis and seizures however these may not always be fully understood on account of the materials often being fairly new and understudied, as such their long term health risks are also not always clearly understood. 2 The United Nations Office on Drugs and Crime (UNODC) and European Monitoring Centre for Drugs and Drug Addiction (EMCDDA) standardised the term "New Psychoactive Substance(s)" and detailed the following sub-categories: synthetic cannabinoids, synthetic cathinones, ketamine, phenethylamines, piperazines, plant-based sub-stances: Khat, Kratom, Salvia divinorum and Miscellaneous: aminoindanes, phencyclidine, tryptamines.
Given the nature of NPSs underhanded production, purposely designed to evade international drug legislation, they are intrinsically marketed and sold as "legal highs".Easily available at 'head shops' (a commercial outlet selling cannabis and tobacco paraphernalia), market stalls and the internet; vendors of NPSs are often operating on the edge of legality by being both vague and creative in their description of the products contents and its purported uses.NPSs may be sold as research chemicals, plant food, bath salts, exotic incenses etc. together with slightly more telling descriptors such as: party pills, herbal highs and smoking blends although these names can often be mercurial, for example, mephedrone (a synthetic cathinone) pre-control was plant food whereas after becoming a controlled substance it was referred to as a 'research chemical'.
Although given these nondescript aliases, NPSs products often have brand names; examples of "legal high" brand names are 'Benzo Fury', 'Afghan Incense', 'NRG-1' and 'NRG-2'.The name or description given to a NPSs or "legal high" product may not always pertain to what is the actual psychoactive substance present, for example mephedrone was detected in products sold as naphyrone or NRG-1 in the UK even after its ban, 3 another survey found 70% of NRG-1 and NRG-2 products examined contained mixtures of substituted cathinones and not, at the time uncontrolled, naphyrone. 4Clearly there are no assurances to the customer of these NPS products that the contents are the same as advertised, furthermore they may be unwittingly violating drug legislation as the contents within are controlled substances.
Abuse of NPSs has been reported to be increasing since ca.2009 and has continued to be an ever growing market 5 emerging at an unprecedented rate something also reflected in the online marketplace with the number of online vendors in the UK increasing by more than 300% between 2010 and 2011. 6New materials made available for abuse appear rapidly and, at times, can gain a 'foothold' in the marketsuch as mephedrone.In 2014, 101 new substances were reported for the first time to the EU early warning system (EWS) run by the EMCDDA up from 81 in 2013 which is also an increase from the 74 substances notified in 2012. 7Of the findings of the EWS synthetic cannabinoids are the most frequently discovered with 102 detected between 2005 and 2013.A graphical representation of NPSs notified to the EWS between 2005-2014 is shown in Fig. 1. 7 The media has reported on numerous deaths related to "legal highs" and given the wide variety of NPS and the everchanging composition of existing products, a completely new field of research has emerged in the continual development of analytical techniques along with presumptive tests and in-thefield sensors.To date, there are reviews on the chemistry, pharmacology and toxicology of NPSs but no comprehensive review of the current techniques for the analysis of these substances has, to-date, been compiled.
In this mini-review a thorough overview of this new analytical field of NPSs is provided which covers: synthetic cannabinoids (most frequently discovered NPS by the EWS), synthetic cathinones; particularly mephedrone (amidst reports by the Crime Survey for England and Wales [CSEW] detailing mephedrone as the most prevalent of abused NPSs) and in lesser detail pieces of interesting research of the other NPSs notified to the EWS (visible in Fig. 1). 7

Synthetic cathinones
Synthetic cathinones are an amphetamine-like cheap alternative to Ecstasy derived from cathinone; an organic stimulant found in Khata plant native to East Africa and the Middle-East and they possess pharmacological similarity to the phenethylamine class of psychoactives (e.g.amphetamine and methamphetamine).[10][11][12] Internationally there has been a tightening of the legislation regarding synthetic cathinone derivatives, for example, cathinones are illegal in the UK as well as Germany, The United States, Canada and many others. 13,14The European Monitoring Centre for Drugs and Drug Addiction's (EMCDDA) Early Warning System (EWS) has reported 74 new synthetic cathinones between 2005 and 2014, with 30 new substances discovered in the year 2014 alone (Fig. 1).Clearly, the epidemic initiated by synthetic cathinones is showing no signs of cessation within the near future hence the development of methods for their detection and quantification is timely and urgently required.Mephedrone in particular, since it's availability for abuse, is popular amongst users of "legal high" products and despite its classification in 2009 reports from the Crime Survey for England and Wales (CSEW) reveal mephedrone was still being abused in England and Wales in 2014.
Popularly known as 'bath salts', 'research chemicals' or 'plant food', synthetic cathinones are sold under, often mercurial, non-descript brand names such as 'Energy' (NRG), Blizzard and Ivory Snow containing warning labels such as 'not for human consumption' or 'not tested for hazards or toxicity' in an attempt to bypass legislative controls.The active component in a "legal high" product can vary wildly, even within the same brand name; 3,10,15 there are no clear assurances to the customer of these NPS products that the contents are the same as advertised (see introduction).
The list of case reports concerning synthetic cathinoneinduced intoxication is extensive and ever increasing.In the United States the number of calls to emergency centres, as a result of synthetic cathinone abuse, increased from 303 to 6100 between 2010 and 2011.A plethora of case reports are reported in the literature and media spanning a sizeable age range, including both of the sexes and include fatalities as well as the curious report of the murder of a goat whilst dressed in lingerie. 16For instance, a female aged 15 had symptoms of nausea, vomiting, altered mental status, euvolaemic hypoosmotic hyponatremia with encephalopathy and increased intracranial pressuremephedrone metabolites were found in her urine. 17A male aged 31 after admitting to taking three 1500 mg packets of "bath salts" and was reported to have hallucinations, paranoia, agitation; elevated serum CPK level, hyperkalemia, dehydration, rhabdomyolysis and acute renal failure.
Considering all the synthetic cathinones discovered, there can be no assertions to which are the being abused but what is evident from the literature is that the most prominent synthetic cathinones found within "legal high" products globally are mephedrone (4′-methylmethcathinone; 4-MMC) and 3′,4′methylenedioxypyrovalerone (MDPV). 15Mephedrone is more prevalent in Europe and MDPV in the United States; 15 a list of the most prevalent cathinone derivatives 18 abused worldwide can be found in Table 1 although the focus of the review will apply generally towards the detection and quantification of mephedrone.
Studying the patterns of NPS abuse can be difficult as it is frequently based upon self-reported user surveys. 19This is potentially problematic as in many instances users are, due to poorly labelled products (see earlier), not in fact aware of the substances they are taking.In light of this, numerous groups are making advances towards screening the current NPSs being abused.A number of revered groups using a range of chromatographic techniques including high performanceliquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS), with LC-MS methods seemingly the preferred and established technique of choice, have published exhaustively upon the laboratory-based analysis of synthetic cathinones, 3, phase I and II metabolites 41,42 and more recently, in light of the often nonenantioselective NPS synthesis, chiral separation of racemic mixtures. 43 In2014 Archer et al. 19 analysed urine samples collected from a night club over one weekend. The mnuscript with its real and imaginative title, "Taking the Pissoira novel and reliable way of knowing what drugs are being used in nightclubs", reported the detection of classical recreational drugs and NPSs such as: mephedrone, 3′-trifluoromethylphenylpiperazine and 2-aminoindane using various chromatographic and mass spectrometric methods.19 Furthermore parent drug/metabolites were also detected for amphetamine, cocaine, ketamine, 3′,4′methylenedioxymethamphetamine (MDMA), mephedrone and 3-trifluoromethylphenylpiperazine (3-TFMPP); this is important as it indicates drugs were being used and not simply discarded into the urinal.19 In the same year, Leffler et al. 44 (located in the United States) analysed 14 separate street samples wherein 10 synthetic cathinones were identified employing a variety of techniques, including gas chromatography with mass spectrometric detection (GC-MS) and flame ionization (GC-FID).44 HPLC direct infusion tandem mass spectrometry (MS/MS) was also used to identify compounds which were not available as reference materials. Ouof the synthetic cathinones detected: 3′,4′-methylenedioxypyrovalerone (MDPV), 3′,4′-methylenedioxy-α-pyrrolidinobutiophenone (MDPBP), 4′-fluoromethcathinone (4-FMC), butylone, mephedrone, naphyrone, 4′-methylethcathinone (4-MEC), ethcathinone, α-pyrrolidinopentiophenone (α-PVP), and 3′-methylα-pyrrolidinopropiophenone (3-MPPP). MDV was the most prevalent, found in five of the 14 samples and ranging from 11% to 73% (w/w) between samples.44 Earlier reports in Denmark, Pedersen et al. 35 presented an automated solid-phase extraction (SPE) and ultra-highperformance liquid chromatography (UHPLC) with time of flight-mass spectrometry (TOF-MS) screening method for 256 illicit compounds in blood and 95 of these compounds were validated with regard to matrix effects, extraction recovery, and process efficiency with the limit of detection (LOD) ranging from 0.001 to 0.1 mg kg −1 .35 Application of the technique to the analysis of 1335 forensic traffic cases revealed 992 cases (74%) were positive for one or more traffic-relevant drugs above the Danish legal limits.Commonly abused drugs such as amphetamine, cocaine, and frequent types of benzodiazepines were the major findings.Nineteen less frequently encountered drugs were detected: buprenorphine, butylone, cathine, fentanyl, lysergic acid diethylamide, m-chlorophenylpiperazine, MDPV, mephedrone, 4′-methylamphetamine, pfluoroamphetamine, and p-methoxy-N-methylamphetamine.35 Even as early as 2011, there have been numerous attempts at constructing screening methods for substituted cathinones in a number of different matrices, Bell and co-workers 29 reported a rapid multi-analyte direct urinalysis LC-MS/MS screening method being able to detect eight analytes including; 4′-methylmethcathinone (mephedrone), 3′,4′-methylenedioxymethcathinone (bk-MDMA, 'methylone'), 4′-methoxymethcathinone (bk-PMMA, 'methedrone') and 3′,4′-methylenedioxypyrovalerone (MDPV).29 Using a dilution of 1 part urine to 4 mobile phase to reduce matrix effects and although not all compounds were completely chromatographically resolved, there was sufficient specificity to allow target analyte identification.All the analytes were readily detected at a concentration of 500 ng mL −1 offering an attractive method for the routine screen of NPSs.29 The global impact of synthetic cathinones is compounded when substances such as mephedrone and MDPV have been detected following sewage-based epidemiology in Chinese 'megacities'.45 In terms of quantification, Santali et al. provided the first fully validated HPLC method for the quantification of mephe-drone 22 where limits of detection and quantification of 0.1 and 0.3 µg mL −1 respectively were reported.Khreit et al. further refined this method enabling the detection of both mephedrone and two novel derivatives, 4′-methyl-N-ethylcathinone (4-MEC) and 4′-methyl-N-benzylcathinone (4-MBC), in seized samples of "NRG-2".In this case the limits of detection and quantification were reported as 0.03 and 0.08 for 4-MEC and 0.05 and 0.14 μg mL −1 for 4-MBC both in their pure form and in the presence of common adulterants such as caffeine and benzocaine.3,23 There has also been work using chromatographic methods on the detection of cathinone based "legal highs" in biological matrices 24,37 in which Beyer et al. were able to detect and quantify 25 designer cathinones in a validated LC-MS-MS method. 37ther work 26 has seen an attempt to screen chronic abuse of mephedrone through GC-MS analysis of hair.The hair was first decontaminated in methylene chloride and incubated overnight in a pH 7 buffer in the presence of deuterated MDMA at 40 degrees Celsius.The work saw 67 hair specimens tested for mephedrone with 13 yielding positive results of concentrations ranging from 0.2-313.2ng mg −1 . 26The work showed that like other stimulant drugs, mephedrone is well incorporated into hair and the analytical method reported appears sensitive enough to reveal occasional to regular use of mephedrone. 26ecently direct analysis in real time mass spectrometry (DART-MS) has been utilised to quantify and characterise the multitude of new and emerging NPSs. 46Solid synthetic cathinone samples (2-FMC, 2-MEC, 2-FEC and 2-EEC) were sampled directly without pre-treatment and positive ion mass spectra were acquired using a DART-SVP™ ion source interfaced to an AccuTOF mass spectrometer.Further advancements in this methodology by the same authors 47 has seen the application of a time-of-flight (TOF) mass analyzer along with in-source collision-induced dissociation (CID) spectra to provide data for presumptive analysis of various synthetic cathinones in a similar fashion to GC-MS analysis. 47The authors scope for this work is to provide a rapid screening method to quickly respond to the rapid evolution of designer drugs and the consequent testing backlogs that develop. 46,47Ion mobility spectrometry (IMS) has also been applied to the screening of an array of NPSs within the literature with acceptable results. 48,49mith et al. 50,51 provided an alternative to chromatography and proposed a novel sensing protocol based upon the electrochemical methods.Of note is the reduction of the cathinone substitutes; mephedrone and 4-MEC with a scope to provide an on-the-spot analytical screening tool with cyclic voltammetry. 50Analysed in pH 4.3 acetate buffer, limits of detection were found to correspond to 11.80 μg mL −1 for 4-MMC and 11.60 μg mL −1 for 4-MEC. 50This work demonstrated for the first time a rapid, accurate, and sensitive method for the quantification of synthetic cathinone components found in seized street "legal high" samples (NRG-2) via the use of an electrochemical protocol utilizing graphite screen-printed electrodes (GSPEs) which was also independently verified with HPLC. 50nteresting developments in the detection of synthetic cathinone derivatives is the use of surface enhanced Ramanspectroscopy (SERS) have also been reported. 52,53In this novel approach, the usually required thin metallic surface (typically gold or silver) was provided by galvanising a British two pence coin with silver.Note that a pre-1992 two pence coin (97% Copper) is required as post-1992 two pence coins are composed of copper-plated steel and have an undefined composition. 52Fig. 2 shows the concept when dendritic structures are evident on the two pence surface, demonstrating proof-ofconcept for SERS detection of mephedrone, MDMA and aminoindane 5′,6′-methylenedioxy-2-aminoindane (MDAI). 52urther developments saw the researchers working towards a new optimization strategy for the SERS detection of mephedrone using a portable Raman system employing a fractional factorial design approach to significantly reduce the statistical experiments whilst maintaining statistical integrity. 53Furthermore, four optimised SERS protocols for which the reproducibility of the SERS signal and the limit of detection of mephedrone were established with an estimated limit of detection of 1.6 μg mL −1 . 53nother alternative to the well-established chromatographic methods, NPS detection has been reported with the use of immunochemistry, Paillet-Loilier et al. 18 noted the use of this technique to test the cross-reactivity of some synthetic cathinones using the semi-quantitative AxSYM amphetamine/ methamphetamine II assay in tandem with Fluorescence Polarization Immunoassay (FPIA).Evaluating the responses from aqueous solutions of 14 substituted cathinones at 1 mg L −1 , 10 mg L −1 and 100 mg L −1 , the authors observe pentedrone, pentylone, α-pyrrolidinovalerophenone (PVP), and 3′,4′methylenedioxypyrovalerone (MDPV) did not react with the protocol.Some synthetic cathinones, however, reacted in the assay at 10 mg L −1 : ethylone, mephedrone, methylone, methedrone, and 4′-methyl-N-ethylcathinone (MEC) scrutiny of this reveals that each of these that did react had the least substitutions on the ethylamine chain suggesting the method has limitations to larger molecules. 18Commercially available enzyme-linked immunosorbent assays have been used to analyse eight synthetic cathinone derivatives amongst 30 designer drugs. 54The test demonstrated cross-reactivity at concentrations at low as 0.15 mg L −1 when tested against the Randox Mephedrone/Methcathinone ELISA kit (RANDOX Toxicology, Crumlin, UK), a protocol recently developed for forensic specific cathinone screening in urine and blood specimens. 54resumptive testing of cathinone derivatives was carried out by Nic Daeid and colleagues 40 as per United Nations recommended guidelines.Various presumptive tests were investigated, however results suggested the Zimmerman test, which relies on the presence of a carbonyl group in close proximity to a methyl group on the same molecule and reaction with 2′,4′dinitrobenzene to form a Meisenheimer reddish-purple colour, was the most consistently effective test method.A small amount of each test sample was placed into a well of a spotting tile and 2 drops of 1% 2′,4′-dinitrobenzene in methanol followed by 2 drops of 15% potassium hydroxide in water were added.Any colour change or other noticeable effect occurring immediately on addition of the reagents was noted and observations were made again after 5 minutes; Specific colour changes were observed in all cases apart from bupropion.Nic Daeid et al. have also reported using stable isotopic fractionation/profiling (isotope ratio mass spectrometry; IRMS), to provide a potentially quantifiable link between the precursor (4′-methylpropiophenone) and the illicit drug product (4′-methylmethcathinone) for a particular manufacturer and synthetic route of mephedrone. 55

Synthetic cannabinoids
Synthetic cannabinoids emerged as a recreational product ca.2008 in the form of aminoalkylindoles such as JWH-018.They were originally investigated by Professor Huffman 56 as therapeutic compounds, however they were subsequently abandoned due to the unwanted psychoactive side effects.Despite many classes synthetic cannabinoids becoming controlled under drug legislation, there are still many which remain legal whilst still posing threat to the population.8][59][60][61] These compounds were first introduced into products known as 'K2' and 'Spice' with the latter having a market range of: Spice Silver, Spice Gold and Spice Diamond.† The products, advertised as incense or smoking mixtures, are typically sold consisting of a few grams of finely cut green/brown plant material as to perhaps replicate the appearance of cannabis whilst being infused with the active synthetic cannabinoid component(s).There are instances of retailers selling the active components as research chemicals (similarly to synthetic cathinones) which arrive as a crystalline powder of high purity. 61here are various case reports to support the literature and media claims that synthetic cannabinoids have psychoactive effects akin to that of cannabis.Indeed, the components of Spice and related herbal products have been identified as aminoalkylindoles originally synthesised by Huffman and Atwood et al. and have demonstrated that JWH-018 is a potent and effective CB 1 receptor agonist. 62nteresting case reports with regards to the effects of the Spice epidemic include a report by Schneir et al., 63 who published case studies on two women admitted to a San Diego (USA) emergency department after smoking Spice "Banana Cream Nuke"disorientated, feeling unusual and "as if they did not know where they were". 63Another report describes three cases of the effects of Spice, 64 all having a negative urine drug screen whilst exhibiting agitation, paranoia and tachycardia.Follow up analysis revealed the urine to contain metabolites of JWH-018 and JWH-073. 64More recent reports also highlight similar observations in adolescents and young adults after intoxication with synthetic cannabinoids. 65Vardakou et al. 66 have given an overview of other case reports 66 and the psychoactive properties of Spice products and "legal highs".
Laboratory analysis revealed the active components of first generation Spice and related products to be, the previously mentioned, aminoalkylindoles such as JWH-018 and also cyclohexylphenols such as CP-47 497.As their popularity rose through sales in so-called 'head shops' as well as on the internet, the substances were legislated as illegal in most countries worldwide; 67 the range of active synthetic cannabinoid components of first generation Spice products can be observed in Scheme 1. Note: the aminoalkylindoles (see Scheme 1) are given the notation of JWH after the academic who first synthesised these compound, Professor J.W. Huffman.
Further confirmation of this came at the end of 2008 when the German company THC Pharma reported JWH-018 was an active ingredient in Spice products. 68Following on from this Auwater et al. 69 and Uchiyama et al. 70 identified and characterized the CP 47 497-C8 (see Scheme 1) as its isomera synthetic by-product in Spice Silver, Gold and Diamond as well as in products named 'Yuctan Fire' and 'Sence' which is reported to have 5 to 10 times more analgesic potency that tetrahydrocannibol. 71n interesting paper from the point of view of the medical staff that have had to deal with the Spice usage patients has a light-hearted title of: "Spice" girls: Synthetic cannabinoid intoxication. 63The authors noted that a urine drugs-of-abuse immunoassay was negative for amphetamines, barbiturates, benzodiazepines, benzoylecgonine (cocaine metabolite), methadone and opiates, oxycodone, phencyclidine, propoxyphene and tetrahydrocannabinoids.The residue of the † Ingredients listed on the packaging of products are as follows -Spice Gold: bay bean, blue lotus, Lion's Tail, Indian Warrior, Dwarf Skullcap, Maconha brava, Pink Lotus, Marshmallow, Red Clover, Rose, Siberian motherwort, Vanilla and honey.Spice Gold Spirit: Leonurus, Cardiaca, Pedicularis, Canadensis, Scutellaria, Latero flora, Athaea officinalis, Rosa damascene, Vanilla planifolia.Spice Diamond: Bay bean, Blue lotus, Lion's tail, Indian Warrior, Dwarf Skullcap, Maconha brava, Pink Lotus, Marshmallow, Red Clover, Rose, Siberian motherwort, vanilla, honey, aroma.

Note the lack of any real ingredients (chemical) and no mention of any aminoalkylindole (JWH compounds) or cyclohexylphenyls (CP compounds)
Scheme 1 Chemical structures of synthetic cannabinoids found in herbal products such as the spice range, 67  patient's Spice product "Banana Cream Nuke" was found to contain the synthetic cannabinoids JWH-018 and JWH-073 through gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography with ultraviolet detection (HPLC-UV).The report highlighted the need for drugs-of-abuse screenings to be able to detect the JWH class of compounds, particularly within a clinical setting.
In Germany Lindigkeit et al. analysed Spice Gold with a GC-MS method wherein the herbal mixtures were ground and put through a two hour Soxhlet extraction with petroleum ether. 58Analysis revealed the samples contained CP 47 497-C8 and JWH-018 until German health authorities on the 22 nd January 2009 prohibited the sale of the active components found in Spicefrom this point JWH-018 was absent from Spice, however it wasn't long until a new analogue, JWH-073, was found to be contained in Spice products. 58Because the manufacturers of such products can readily change the active components in Spice, a rapid method of detecting prohibited compounds in the complex mixtures is highly sought after.
To this end, Emanuel and co-workers 68 reported for the first time the components of Spice "Gold Spirit" using GC-MS (following a simple liquid extraction) alongside the analysis of Spice "Gold" and "Diamond"; at the time the three most popular Spice products used.Results indicated that Spice "Gold" contained CP 47 497-C8 along with ethyl vanillin, α-tocopherol and γ-tocopherol whereas Spice "Diamond" contained caffeine, α-tocopherol, γ-tocopherol, palmitic acid along with CP 47 497-C8 and JWH-018.As for Spice "Gold Spirit", JWH-018 and α-tocopherol were found to be present. 68ther work has of course followed on the analysis of Spice and related herbal products for instance Uchiyama and coworkers 59 who analysed 46 different herbal products with 44 having synthetic cannabinoids as determined via GC-MS and LC-MS.Two major cannabinoids were found; [2-hydroxy-4-(2-methylnonan-2-yl)phenyl]cyclohexan-1-ol (cannabicyclohexanol) and JWH-018 and the analysis of the herbal product (amount of NPS per gram) were found to range from 1.1 to 16.9 mg g −1 and 2.0 to 35.9 mg g −1 respectively. 59n addition to the identification of the chemical components contained within the Spice product range there is a need to understand the effects of the synthetic cannabinoids on the human metabolism.Sobolevsky 72 reported for the first the time, urinary metabolites of JWH-018; clearly highly useful for the analysis of patients admitted to emergency departments and for the development of point-of-care tests (see the story of the "Spice girls" earlier in this mini-review).Using LC-MS and GC-MS, two main monohydroxylated metabolites were identified which are almost completely glucuroconjugated with minor metabolites such as N-despentyl hydroxy-, carboxy-, dihydroxy-, and reduced di-and trihydroxy-metabolites. 72 It should be noted the parent compound (JWH-018) was reported to not be detected in urine. 72The authors observed that there are two main metabolites that are valuable for detection of JWH-018 in post-administration urine and LC-MS is a more useful technique as minor metabolites can also be analysed to support analytical findings. 72Different analytical approaches on Spice and related products have been reported [73][74][75][76][77][78] with literature reporting the presence of new cannabimimetic compounds. 60,79,802][83][84][85][86][87] Further work by Moran et al. 88 has extended the work of Sobolevsky 72 and validated an LC-MS/MS method for the quantitation of the human urine metabolites of JWH-018 and JWH-073.The work highlighted 6 metabolites for each molecule with the primary metabolites being distinguishable between JWH-018 and JWH-073.The authors have also extended this using a solid-phase extraction approach. 89One criticism of the above work exploring the metabolites in urine is the limited population studiesclearly larger studies will be needed to further understand the pharmacology of synthetic cannabinoids.][92][93][94] A different strategy has been to analyse cannabinoids in hair to show long term past consumption. 95To this end, Hutter et al. 96 reported the hair testing of 22 synthetic cannabinoids in human hair.The methodology involves a simple ultrasonication of the hair sample in ethanol and has a limit of quantification (LOQ) of 0.5 pg mg −1 . 96Perhaps more interestingly, synthetic cannabinoids have even been found in the urine of US athletes (although its use to enhance performance is questionable.).Urine samples were collected from 5956 athletes and analysed via high performance-liquid chromatography-tandem mass spectrometry (HPLC-MS) for the presence of JWH-018, JWH-073 and their metabolites. 97In 4.5% of the samples, metabolites of both synthetic cannabinoid compounds were detected; metabolites of JWH-018 and JWH-073 (50%), JWH-018 (49%), and only JWH-073 (1%) were detected in positive samples.
The focus of the research above has focused on laboratory based instrumentation, rightly so in order to unambiguously quantify NPSs but as highlighted in the case of the "Spice girls", synthetic cannabinoids do not react using tradition THC immunoassay tests.To this end Arnston et al. 98 have designed two enzyme linked immunosorbent assays for detection of JWH-018 and JWH-250 in urine.The assay of JWH-018 has significant cross reactivity with several synthetic cannabinoids and their metabolites contrary to the JWH-250 assay which exhibits limited cross-reactivity.To start, assays are calibrated at 5 ng mL −1 with the 5-OH metabolite of JWH-018 and the 4-OH metabolite of JWH-250.To validate the method, 114 and 84 samples of urine for JWH-018 and JWH-250 respectively were used and confirmed by using liquid chromatograph tandem mass spectrometry (LC-MS/MS) testing for metabolites of JWH-018, JWH-019, JWH-073, JWH-250 and AM-2201.Accuracy was deemed to be greater than 98% with 95% sensitivity and specificity for both assays.
Another approach of interest is a presumptive test marketed by "Narcotic Testing Supplies & Equipment Store". 99The test works by inserting a small quantity of a suspected sample into a plastic ampoule containing 25 μL reagent and 150 mg of specially treated absorbing crystals (sodium 36%, potassium iodide 98% and 0.2% ethanol) stirring and comparing the colour of the liquid to a pre-determined colour chart clearly visible from Fig. 3 however the specificity of such a screening test is questionable. 99s components of Spice and related substances become banned, they are replaced with a compound which exhibits similar psychoactive properties yet negating the effectiveness of the newly introduced ban, see the paper: "Spice: A Never ending story?" for example. 58As such there is an urgent need for a faster laboratory method; Emanuel et al. reported the use of solid probe mass spectrometry alleviating the need for any sample pre-treatment such as liquid-liquid extraction. 68Since α-tocopherol is always present in the Spice herbs range, the authors demonstrated that once α-tocopherol was subtracted from the obtained spectra, the fragmentation patterns of CP 47 497-C8 and JWH-018 become 'visible'. 68This screening methodology is useful for the rapid analysis of the prohibited substances within the Spice product range (as well as related substances) with a positive response nullifying the need for any pre-treatment step (such as liquid-liquid extraction) allowing a full quantification via GC-MS or similar approaches i.e.LC-MS.Work from Lesiak et al. 100 has also attempted to rapidly detect synthetic cannabinoids without the need for sample preparation with the use of direct analysis in real time mass spectrometry (DART-MS) 100 being able to screen for AM-2201, JWH-122, JWH-203, JWH-210 and RCS-4.

Miscellaneous
As reported in the introduction, the new psychoactive substance epidemic is an ever growing market with a vast array of new materials discovered each year. 7To cover every known substance is beyond the scope of this review however; in this section, interesting pieces of research from around the world will be covered.
Piperazines N-Benzylpiperazine (BZP), the structure of which is shown in Scheme 3, is known to be a central nervous system stimulant with its effects reported to be similar to amphetamine in that it also triggers the release of dopamine and norepinephrine whilst inhibiting the uptake of dopamine, norepinephrine and serotonin. 111Although BZP is structurally similar to amphetamine it is reported to have only one-tenth the potency. 111arketed as a 'party pill' before legal restrictions BZP was viewed as a safe alternative to amphetamines such as MDMA, 112 however recently it has varying degrees of legislative control internationally. 113Its appearance in "legal high" samples is still reported 114,115 however after being made illegal the prevalence of its use has declined; for example in New Zealand after being made a prohibited substance in 2008, the use of BZP amongst the general population dropped from 15.3% in 2006 to 3.2% in 2009. 116n the UK, the first deaths associated with BZP and 3-TFMPP were three separate fatalities wherein one of both of the drugs were confirmed to be present although not determined to be the direct mechanism of death. 117Dickson et al. 118 reported that BZP, 3′-TFMPP and MCPP are present in ecstasy tablets since the former, in some nations, is a legal alternative to MDMA.The authors analysed 251 MDMA posi-tive urine samples using GC-MS via a liquid-liquid extraction and pentafluoropropionic anhydride (PFPA) derivatisation as sample pre-treatment to screen for 33 drugs potentially present. 118In 36% of the sample, drugs other than MDMA were found to be present; BZP, 3-TFMPP and MCPP were detected in 15%, 7% and 1% of the samples respectively. 118 wide array of analytical approaches have been reported by many different authors such as LC-MS, 24,119 capillary electrophoresis, 120 HPLC-fluorescence, 121 LC with diode array, 122,123 GC-MS [124][125][126] and chemiluminescence. 127Arbo and coworkers 128 provided a thorough overview of piperazine compounds as drugs of abuse with the full range of analytical techniques and matrices applied, readers are directed to this paper. 128t is clear, something that is generally the case with all "legal highs", confirmatory laboratory based analysis is well developed.Lesser developed, however, are approaches that could adapted for used in-the-field or within a clinical setting where a near-instantaneous response is required.To this end, currently there are no immunoassays for the detection of piperazines derivatives 128 and cross-reactivity of these compounds in fluorescence polarization immunoassay using AxSYM®, amphetamine/methamphetamine assay has been reported. 129ecently Philp et al. 130 have reported on the development and validation of a specific colour test using 1′,2′-naphthoquinone-4-sulphonate (NQS) forming an intense bright orangered complex with BZP at room temperature.The authors reported that common cutting agents such as glucose and caffeine did not affect the test.3-TFMPP, MCPP, pCPP, MeOPP and piperazines produced an orange-red colour change where the apparent brilliance of the BZP-NQS complex made it apparently to be distinguishable from the other colour changes with the potential cross-reactants.

Aminoindanes
Aminoindanes are a group of synthetic compounds characterised by the presence of a phenethylamine skeleton, they are currently not controlled globally 131 and have more recently been found to be contained in "legal high" products sold as powders akin to synthetic cathinones. 132,1332-Aminoindane  has a basic ring structure that is similar to amphetamine (and therefore by proxy, substituted cathinones also) that can be chemically modified and the following derivatives (Scheme 4); 5′,6′-methylenedioxy-2-aminoindane (MDAI), 5′,6′-methylenedioxy-N-methyl-2-aminoindane (MDMAI), 5′-iodo-2-aminoindane (5-IAI), and 5′-methoxy-6′-methyl-2-aminoindane (MMAI) have all reportedly been found in "legal highs". 132 number of aminoindane compounds have been thoroughly characterized by Casale and Hays 134 who provided analytical protocols in the form of NMR, MS and IR for 5-IAI, 4-IAI, their synthetic intermediates and impurities in order to assist forensic analysts.134 There is other work that reports a LC-MS/MS screening method for 26 analytes, 34 including MDAI, and such an approach is designed to provide screening, within a clinical toxicology setting, for the potential misuse of "legal highs" via analysis of urine.34 Particularly of note, work by Elie and co-workers reports that microcrystalline identification of MDAI, mephedrone and N-benzylpiperazine (BZP) is possible.114 In this protocol the illicit compound is dissolved into methanol and diluted with water to produce a content of 10% (v/v) with mercury chloride (10 g L −1 + 10% methanol) used as the microcrystalline agent.114 This approach involves dropping 10 μL of the drug solution with 10 μL of the reagent solution onto a glass slide; the resulting structures were optically imaged following assisted nucleation (gently swirling a plastic pipette tip in the freshly mixed drop). 114Fig. 4 shows the observed crystal structure which is compared to the crystal structure of illicit drugs.The MDAI free base (Fig. 4bi) was found to form flat serrated blades of various dimensions which become irregular with increasing sizes.Smaller crystals are observed to be single blades whereas larger crystals develop two dimensional bunch structuresafter drying larger blade crystals are evident.It was noted that crystals grew within 60 s following assisted nucleation indicating the potential for a fast presumptive test strategy.114 The uniqueness of these tests were determined through comparisons of MDAI structure with a range of illicit drugs, indicating that potentially this approach is feasible to identify the MDAI structure in a real sample containing other illicit drugs. To his end the authors 114 purchased "legal high" samples and utilised their microcrystalline presumptive test approach which when collaborated with FTIR/GC-MS.

Salvinorin A (Saliva divinorum)
Salvia divinorum is a hallucinogenic psychoactive herb local to Oaxaca in Central Mexico and for centuries has been used by cultures indigenous to the region. 135,136This rare member of the mint family is also known as 'magic mint' and more colloquially: 'ska Maria', 'ska Pastora', 'hierba de Maria', 'hojas de la Pastora' all names which pertain to the belief that S. divinorum is the reincarnation of the Virgin Mary. 137The use of this plant as a psychoactive substance has spread globally, its major constituentsalvinorin A (SA) is a known selective opioid antagonist and to this end emphasis in the literature has been put on detecting SA. 135 A dosage between 200-500 μg of SA has been found to induce profound hallucinations with feelings of physical or mental displacement as well as experiencing extraordinary illusions. 138Recently studies have postured SAs effects involve the endocannabinoid system. 139o analyse intact S. divinorum leaves for the presence of SA there has been the employing of both thin layer chromatography using desorption electrospray ionization mass spectrometry (TLC-DESI-MS) 140 and thin layer chromatography teamed with gas chromatography/mass spectrometry (TLC-GS/ MS). 141By utilizing these techniques, the authors of both techniques were able to confirm the presence of salvinorin A in a submitted plant material suspected to be Salvia divinorum. 140,141ichini and co-workers 142 attempted the detection of Salvinorin A in different biological matrices opposed to the solid leaf matter.Utilising a gas chromatography mass spectrometric protocol, it was applied to detecting SA in plasma, urine, saliva and sweat. 142Following validation with 17-alphamethyltestosterone as an internal standard the method was applied to the analysis of urine, saliva and sweat from two consumers after smoking 75 mg plant leaves.Salvinorin A was detected in urine (2.4 and 10.9 ng mL −1 ) and saliva (11.1 and 25.0 ng mL −1 ), but not in sweat patches from consumers. 142he quantification of SA in plasma and cerebrospinal fluid (from a rhesus monkey) has also been attempted and successfully completed using a negative ion liquid chromatographymass spectrometry atmospheric pressure chemical ionization (LC-MS/APCI). 143Using the United States Food and Drug Administration (FDA) guidelines the authors of the method concluded the technique had a lower limit of quantification (LLOQ) of 2 ng mL −1 for 0.5 mL of plasma samples over the linear range 2-1000 ng mL −1 . 143

Mitragynine (Kratom)
Mitragynine is an indole alkaloid derived from the plant Mitragyna speciosa which is indigenous to Thailand and other Southeast Asian countries.This is a common "legal high" and is known commonly as Kratom which is also the chemical's Thai name.The leaves of the M. speciosa were historically used as an opium substitute as well as being used traditionally by villages in southern Thailand as a medicine for diarrhoea, muscle pain and hypertension in addition to also being used by agricultural workers and labourers to relieve tiredness and improve efficiency. 144Its study remains pertinent as reports of a fatality associated with Kratom are as recent as 2013. 145nterestingly, mitragynine is the major constituent of Kratom reported to be 66.2% based on the crude base from the young leaves. 146,147Levels of mitragynine in adults plants from Thailand have been reported to be approximately over 60% whereas in Malaysia only over 10%.Paynantheine and the mitragynine diastereomer speciogynine were the second most abundant alkaloids and the mitragynine diastereomer speciogynine was the third abundant alkaloid in both plants. 1480][151] Unlike the case of other NPSs reported in this review where they have emerged and analytical techniques have had to be developed/invented for their quantification, mitragynine, due to its historical use analytical methods already exist and are generally applied to facilitate pharmacological studies.To this end, Janchawee 144 reported the first analytical methodology utilising HPLC-UV.A linear range of 0.1-10 μg mL −1 was reported with a LOD of 0.03 μg mL −1 and LOQ of 0.1 μg mL −1 .Their protocol was applied to determine the pharmacokinetic characteristic of mitragynine in the serum of rats following oral administration.
As the leaves of Kratom became sold as "legal highs" in many other countries Kikura-Hanajiri and colleagues 146 reported the detection of mitragynine and 7-OH-mitragynine (oxidative derivatives of mitragynine) 152 in 13 "legal high" products using LC-ESI-MS.The authors found that 11 of the 13 products were found to contain mitragynine and 7-OH-mitragynine with their content found to range from 1 to 6% and in the latter 0.01 to 0.04%. 146Other researchers have directed research to study the methods of mitragynine in biological matrices using LC-MS [153][154][155] and UHPLC-UV. 156,157rom inspection of the literature, it is evident that there are multiple ways for the detection and quantification of Kratom ingestion/consumption with detection levels as low as 0.02 µg mL −1 . 158For example Arndt and co-workers reported upon a case of a drug and rehabilitation centre reporting an analysis for Krypton (another name for Kratom) in the urine of a former opiate addicted woman. 159The immunological drug screenings were performed with test strips and a cloned enzyme donor immunoassay wherein alkaloids and tramadol metabolites were analysed by LC-MS/MS.The immunoassays yielded negative responses for amphetamines, barbiturates, benzodiazepines, benzoylecgonine, buprenorphine, ethylgluconoride, methadone, opiates, oxycodone and THC-COOH just as the test strips were negative from tramadol and its metabolites.The LC-MS/MS detected the alkaloids typically found in Kratom mitragynine, speciociliatine, speciogynine, mitraciliatine and paynantheinedetection of these alkaloids served sufficient proof of Kratom abuse and after confrontation with data the patient admitted to several infusions of the plant. 159

Conclusion and future challenges
The work described in this review demonstrates the range of new analytical methods and techniques applied to the detection and quantification of NPSs, which have recently emerged on the recreational drugs market.Given the rapidly evolving nature of the recreational drugs market, in terms of the number of new substances being identified (101 new substances, in Europe, in 2014); the ease at which these substances are available through on-line vendors or "head shops"; the freely-available information regarding NPS production and/or pharmacology and the lack of globalised drug/precursor control legislationmakes the current analytical, forensic and legal challenges clearly apparent.These issues coupled with the limited availability and range of certified primary reference standards; fully validated, simple and cheap laboratory-based analytical methods and selective and sensitive infield testing technology highlights the growing gap in knowl-edge and necessitates economic investment and focused research in this underfunded area.
Future advances can be expected in the following areas: (i) Design and development of miniaturised in-field detection systems for NPSs in bulk samples or adulterated products (such as alcoholic drinks); (ii) Rapid, non-evasive bioanalytical methods for detection of the principle metabolites of common NPSs; (iii) simple, selective and validated laboratory-based chromatographic methods for the discrimination of new psychoactive substances, their isomers and their principle metabolites in biological matrices and; (iv) impurity profiling and/or source identification of common NPSs.
Clearly, the "war on drugs" is showing no sign of relenting in the near future and the principle challenge facing law enforcement agencies is to be 'one-step-ahead' of the clandestine drug manufacturers.By working collectively, analytical chemists, policy makers, law enforcement and forensic practitioners can suitably identify potential classes of molecules that may become the next generation of NPSs and develop advanced methods/technologies for the simultaneous detection/quantification of these substances thereby legislating against potentially dangerous compounds before they pose a serious threat to human health.

Fig. 1 A
Fig. 1 A graphical representation of new psychoactive substances notified to the EWS between 2005-2014.Reproduced from ref. 7 with permission of the EMCDDA.

Fig. 2
Fig. 2 Characterisation of galvanic displacement.The optical image (top left) shows a clean British 2p coin, with silver deposited onto its surface.(A) Shows an SEM of the rough surface of the two pence after cleaning.The SEM in (B) shows the silver dendritic structures that are formed on the coins surface once 10 μL of AgNO 3 was left to mature for 20 s at room temperature (23 °C).The fern like structures are magnified in (C) and show that secondary crystalline domains grow perpendicular from a primary silver backbone. 52Reproduced from ref. 52 with permission of The Royal Society of Chemistry.
Scheme 1 Chemical structures of synthetic cannabinoids found in herbal products such as the spice range,67 scheme reproduced from ref.67 with permission from UNODC.

Fig. 3
Fig. 3 Visual representation of synthetic cannabinoid presumptive test, reproduced from ref. 99 with permission of Narcotic Testing Supplies & Equipment Store.

Scheme 3
Scheme 3 Benzylpiperazine and other piperazines derivatives which have been historically abused.

Table 1
List of the most common synthetic cathinones, recreated from ref. 18 with permission of DovePress