hydrogels from alkenyl succinic ester terminated bola-amphiphiles for large-scale applications †

The present study describes an economic and scalable approach to aqueous mesophases from bolaamphiphiles (BA) obtained via nucleophilic addition of dimer fatty acid based a,u-polyesterdiols (PES) on cyclic acid anhydrides and conversion of the carboxylic end groups into ammonium salts. Novel bolaamphiphilic head groups are introduced using alkenyl succinic anhydrides (ASA). The additional terminal hydrophobic side chains favour the self-assembly of polymeric BA of different molecular weights into nanoscale anisotropic objects, their shape and ordering into nematic or lamellar-like phases being dependent on the length and structural uniformity of the ASA chains. Corresponding diester based on C15 (hydrogenated bisphenol-A, HBA) and C8 (1,4-cyclohexanedimethanol, CHDM) spacers have been prepared and the self-assembly of the resulting BA in water has been studied using SAXS, H-NMR and optical polarization microscopy. While the rigid C8 spacer impedes any ordering, ASA capped C15 tends to form ordered hydrogels over extended regions of the phase diagram that resemble mesh phases and La/L3 polymorphism. Rheological and simulation results confirm the presence of elastically responding bicontinuous morphologies and biased porous assemblies resembling interconnected mesh phases. Both the use of the dimer fatty acid based spacer as well as of ASA head groups open up large-scale applications of ordered liquids (or hydrogels) as a formulation basis for e.g. films, coatings and adhesives.


Introduction
Self-assembly of amphiphiles is a convenient strategy to obtain functional nanostructures useful as so templates for the fabrication of (porous) solids, 1 for the encapsulation of actives or food additives 2 or for advanced applications like optoelectronics. 3Although numerous examples for thin lms that are anisotropic on the mesoscale are known, 4 in the context of large scale applications like organic coatings for automotives or aviation the use of ordered materials for mesoscopical, anisotropic layers have barely been reported.However attractive property proles, e.g.combined mechanical reinforcement, diffusion barrier and pigment orientation might be realized making use of a lyotropic mesophase as an intermediate step of the formulation as we have shown recently: in the presence of a polyester dispersion individual platelets of a layered double hydroxide adopt a nematic like ordering in the aqueous phase. 5owever the limited extension of nematically arranged platelets perpendicular to the director led to a superimposed lamellar assembly on larger length scales which was transferred into the solid coating in the form of an ordered array of polymer intercalated platelets.In this study we describe an approach to obtain lyotropic ordering of polymeric bola-amphiphiles in the aqueous phase, namely extended layered structures, thus mimicking robust lipid membranes of archaebacteria that consist of bola-amphiphiles, a sort of amphiphiles comprising two hydrophilic heads connected by a hydrophobic spacer.
Since early studies on bola electrolytes 6 and on cationic bolaform detergents 7 as well as the pioneering investigations of Vittorio Luzzati on the polymorphism of several extracted archae-bacterial bola-amphiphiles 8 numerous synthetic systems have been prepared and their self-assembly studied, 9 the main aspects investigated being the nature of the head groups (non-, an-or cationic) and the spacer (aliphatic, aromatic, and uorinated), the molecular architecture (symmetry, length, bulkiness, and shape) or the concept of supra-amphiphiles featuring specic binding sites and the role of the counter ion. 10 Common to all is the molecular uniformity (purity) of the organic mesogen resulting from tedious synthesis and purication procedures that are economically not affordable for large-scale industrial application.Furthermore low molecular weights and thus high proportions of hydrophilic, possibly ionic groups are incompatible with coating applications which are thought to provide resistance against humidity with regard to swelling and (ion) permeability.
We found that ill-dened bola-form polyesters prepared from inexpensive raw materials self-assemble in aqueous solutions into ordered structures and could thus overcome the economic and technological constraints.Besides hydrophobicity stemming from dimer fatty acids in the polymer backbone we have investigated the effect of hydrophobic aliphatic side groups in the aand b-position to the ionic head group on the self-assembly.The role of double-stranded bolaform architecture has already been addressed in the context of vesicle formation of symmetrical glycosylated bola-amphiphiles 11 making reference to similar asymmetric molecules where hydrophobic chains were attached at the anomeric site of the head groups' furanose ring. 12In the present study the head groups result from nucleophilic addition of an a,u-polyesterdiol (PES) on alkenyl succinic anhydrides (ASA) which have been proposed for numerous technical applications and are used on a large scale, e.g. as sizing agents for starch 13 and cellulose 14 in the food and paper industry respectively.Contrary to succinic and maleic anhydride head groups 15 as well as dimer fatty acids 16 commercially available as technical mixtures of isomers on the ton scale -ASA derived head groups have not yet been proposed for the synthesis of bola-amphiphiles to the best of our knowledge.
This article is divided into four parts.The rst chapter describes the synthesis and molecular diversity of the polymeric bola-amphiphiles.The formation and characterization of their aqueous dispersions are given in the second chapter.The third section addresses the role of different ASA derived terminal side chains, which was studied in monomeric diester based bolaamphiphiles.Finally the systems studied are put into the context of shorter homologues that resemble Gemini surfactants as well as non-bola type amphiphilic polyesters.

Synthesis
Polyester 1 (PES) was synthesized from an equimolar mixture of A1 and A2 together with hydrogenated bisphenol-A using a diol to diacid ratio of 2.26 under nitrogen and stirring at 190 C and distillation of water until an acid functionality below 0.15 meq of the reaction mixture was reached.Polyester PES-NB was obtained from the addition-condensation reaction of 72.7 g 1 (2.55 meq hydroxyl) with 8.35 g trimellitic anhydride under nitrogen and stirring and water distillation at 160 C until an acid functionality of 0.62 meq was reached.For the bolaamphiphiles 3, 4 and 5, the polyester 1, hydrogenated bisphenol-A and 1,4-bis (hydroxymethyl)cyclohexane respectively were reacted with sub-stoichiometric amounts of 0.9 equivalents cyclic anhydride 2 per hydroxyl at 95 C in 2-butanone under stirring and under dried nitrogen until 100% anhydride conversion according to titrations under aqueous versus ethanolic conditions.

Dispersion
All polymeric amphiphiles 3 and PES-NB were neutralized using 0.95 equivalent dimethyl ethanolamine per acid at 80 C followed by addition of water and distillation of 2-butanone until a content <0.5 weight% was reached.Water was added to adjust the amphiphile concentration, expressed as the w value for the ammonium salts, before the mixture was cooled to ambient temperature.For amphiphiles 4 and 5 the same procedure was applied using a minimum temperature of 60 C.

Characterization
Small-angle X-ray scattering (SAXS) was recorded on the ID02high brilliance beamline of ESRF, Grenoble, France using monochromatic radiation (l ¼ 0.1 nm), a source-sample distance of 55 m and sample-detector distances (max range 1 to 10 m) to cover a typical q-range of 0.3 to 4.3 nm À1 (0.03 to 6 nm À1 for 3a and 3b).Intensities were corrected for empty mica platelets that were used as sample holders.
2 H-NMR spectra were recorded with an Agilent VNMRS 500 MHz spectrometer at a resonance frequency of 76 717 MHz, with a spectral width of 7700 Hz, 80 transients, a pulse width of 20 ms (p/2) and an acquisition time of 0.3 s.Samples were preequilibrated for 10 min.The heating rate was 2.5 C min À1 .Polarization light microscopy (PLM) pictures were taken by using an Olympus BX51 microscope equipped with an UM Plan FI-lens and a XC10 digital camera.A droplet of the sample, conditioned at room temperature was prepared between a glass slide and a cover slip.For temperature-dependent PLM a Leitz Orthoplan light microscope equipped with a Linkam temperature control unit and a liquid nitrogen cooling unit LNP, TMS 94 was used.
Cryogenic atomic force microscopy (cryo-AFM) was carried out using a Bruker Dimension Icon equipped with a FastScan ScanAsyst scanner and a Veeco DI TA controller.Samples were prepared using a Reichert Ultracut S sectioning system equipped with a FCS cryo extension.A droplet of the sample, conditioned under ambient conditions for 10 min, was deposited on a metal attachment and microtomed at À80 C. The frozen, truncated sample was transferred to the precooled stage (À20 C).AFM recording started aer complete sublimation of formed ice crystals (approx.15 min) using a frequency of 15 Hz.Cryogenic transmission electron microscopy (cryo-TEM) was performed on a FEI Tecnai G 2 electron microscope using 200 kV as the accelerating voltage.Ultrathin sections (70 nm) of the sample were cut at À80 C using a Leica UC6 ultra-microtome equipped with a Leica EM FCS cryo chamber and a diamond knife.
Rheological characterization of the bola-amphiphiles 4 was performed on an Anton Paar MCR302 CP25/1 rheometer for amplitude and frequency sweeps at 23 C using a cone plate distance of 0.05 mm at a constant frequency of 1 Hz and a constant amplitude of 0.1 mrad covering 8.8 Â 10 À3 to 1000 mrad and 1 and 100 Hz respectively.Temperature dependent measurements were performed on an Anton Paar MCR501 CP50/1 rheometer using a cone plate distance of 0.099 mm at a constant frequency of 1 Hz and an amplitude of 5.8 mrad and applying a constant heating rate of 1 K min À1 covering 20-90 C.
Rheological characterization of PES-NB was performed using a Haake MARS system (Thermo Scientic) with a 2 titanium cone and a glass plate (gap width 70 mm), and temperature-controlled by using a Haake thermostat.For temperature dependent measurements appropriate steps were dened.Only data that fulll the harmonic approximation aer stabilization (DT < 1 K) were used.These were taken at 10 Pa and 100 Pa and a frequency of 1 Hz over a period of at least 3 min on each stress level.Due to high viscosity of PES-NB dispersions the gap might not always be lled completely in its outer part.Therefore absolute values of G 0 and G 00 may be offset and should be compared directly only in individual measurement runs.
Simulated systems contained various isomers of 4c, counter ions and water yielding a total N ¼ 16 000 atoms their proportions adjusted to concentrations of the organic phase (4c plus counter ions) in the range of 0.3 # w # 0.6.All molecular dynamics simulations were carried out using LAMMPS ‡ (1) within an NPT ensemble, i.e. at constant pressure and temperature (p ¼ 1 atm, T ¼ 298.15 K) and an integration time step of one femtosecond.Interatomic potentials were described by the COMPASS § force eld and VMD{ was used for analysis and visualization.

Results and discussion
Amphiphilic polyesters: synthesis and structure All polymers are based on a hydrophobic a,u-polyesterdiol (PES) which is obtained from the esterication between hydrogenated bisphenol-A (HBA) and a 1 : 1 molar mixture of hydrogenated phthalic anhydride (HPA) and dimer fatty acid (DFA).Nucleophilic addition of the terminal hydroxyl groups on a cyclic anhydride (SA, MA, ASA, and TMA) converts PES into a telechelic carboxylic acid terminated polyester, namely a polymeric bola-amphiphile PES-BA (Fig. 1).For the non-bola polyester PES-NB ring opening of TMA is followed by polycondensation which formally yields chain extended PES with residual mid chain carboxylic acid groups.Molecular weight distributions from SEC (Fig. 2a) show that PES 1 consists of numerous different species reecting both the mass and size difference between the C8 and C36 based synthons A1 and A2 respectively as well as a different reactivity between HPA (anhydride) and DFA (free acid) towards the hydroxyls of HBA.The latter was chosen because of its combined hydrophobicity, stiffness and hydrolytic stability of the formed ester bond.However this goes along with a low reactivity in the esterication and anhydride ring opening which entails prolonged synthesis compared to primary alcohol esterication.For compensation we used an unusually high diol to diacid ratio (2.26) that in theory does not allow polymer formation.Without further analytical evidence relating to the chemical nature of the different SEC fractions it is evident that PES must consist of oligomers besides diester and excessive HBA.This is corroborated by the size exclusion chromatogram of 4c which is shown for comparison in Fig. 2b.Thus non-equilibrium reects kinetically favoured esterication between HPA (ring opening) and "trans" isomers of HBA (least steric hindrance).It has to be noted that the HBA quality we used consists of three isomers with respect to the hydroxyl ring position.Related to bisequatorial congured isopropylidene moieties equatorial and axial positioned hydroxyl groups account for a dominating trans,trans isomer (w ¼ 0.5), a cis,trans isomer and a minor portion (w ¼ 0.1) of a cis,cis isomer (see ESI †).Assuming the fastest reaction of trans,trans HBA with HPA due to least steric hindrance and ring opening and a higher reactivity of DFA compared to that of the HPA-HBA addition product (steric hindrance in the latter), the most probable intermediate would be HPA-HBA-DFA which in turn would preferentially consume trans,trans-HBA followed by trans,cis-HBA.Thus residual HBA should consist of enriched cis,trans-and cis,cis-HBA.These most probable species are highlighted in a list of all combinatory conceivable bola-amphiphiles of exemplary 3c indicating molecular weights and lengths in all-trans conguration (Table S1 †).Molecular diversity in PES additionally stems from nonuniformity of DFA that comprises numerous isomers regarding the 'connecting' moieties, best described as products of an enereaction and a cycloaddition. 16Thus linear and cyclic increments with varying proportions of saturated aliphatic, unsaturated and aromatic bonds depending on process parameters and optional post hydrogenation are common.The quality we used comprises minor amounts of mono-and tri-functional species (cf.Experimental part).Regarding the SEC curves (Fig. 2) we emphasize that no absolute molecular weights can be deduced due to PMMA standards.However comparing distributions of PES with different PES-BA (3a, 3c) and diester (4c) it is obvious that the prominent peaks fall into the range of theoretically deduced molecular weights (vide supra, cf. Table S1 †).Noteworthy the shape of the curves found for 3a and 3c indicates an impact of the head groups on the hydrodynamic radius.As expected for PES-NB, the mean molecular weight and the dispersity (M w /M n ) are considerably higher (Fig. 2c).
Regarding the amphiphiles of series 3 and 4 molecular complexity increases aer ASA and TMA ring-opening which yields ester groups bearing an alkenyl chain in the aor bposition or the pristine carboxylic acid group in the para or meta position respectively (cf.Fig. 1).Finally for a given ASA type branching and cis/trans isomerism of the aliphatic group adds to molecular plurality.
Therefore we studied the impact of the length (C8, C12, C16/ C18) and the structure (linear, branched) of the alkenyl side group.Numerous different ASA qualities are commercially available.Typically synthesized from maleic anhydride and a-olens in an ene-reaction it is known that mixtures of branched products are obtained under process conditions where the olenic double bond is prone to migrate chain inwards. 17Thus at least three different materials are labelled "dodecenyl-SA": a rather pure chemical comprising two (cis,trans) linear C12 chains (2d), a mixture of isomers with respect to branched C12  chains stemming from an undened precursor or an in situ scrambled linear a-olen (2e) and nally an isomer mixture based on tetrapropenyl (2f).While the short C8 ASA (2c) consists of two linear species (cis,trans), GC analysis of C16/C18 ASA (2g) shows two sets of numerous isomers for the two different chain lengths (not shown).We compared these ASA terminated PES-BA with those bearing succinic (2a), maleic (2b) half ester as well as the addition product from TMA (2h).

Mesophases from PES-BA
For dispersion into water the amphiphile acidic groups were transformed into the ammonium salt of dimethyl ethanolamine.The PES-BA (3a-3h) are dispersed in water covering a concentration range of 0.3 < w < 0.8 (w ¼ weight fraction of PES-BA).For this purpose samples were taken from a dilution process of a concentrated stock solution (w $ 0.8) at 80 C. However with succinic and maleic terminated PES-BA (3a, 3b) homogeneous mixtures could not be obtained for mixtures below w ¼ 0.5 which was conrmed by contact preparations for polarized light microscopy (PLM) under ambient conditions.These showed a quick formation of a myelin like phase near the borderline that did not change within hours (Fig. 3a).We interpret these ndings with the formation of extended lamellae.These impede or signicantly retard further dilution but can swell.Indeed SAXS peaks in the ultra-small angle region for both systems correspond to 100-200 nm large objects (shown for 3a, Fig. S2 †).Such a huge spacing of swollen BA based lamellae is not unusual 18 and has also been reported for dilute ASA-water systems. 19Upon standing over several months 3a (w ¼ 0.54) macroscopically phase separates into an isotropic phase (no birefringence, no peaks in SAXS) beneath a lamellar phase as indicated by three harmonic peaks with a corresponding repeat distance of 7.9 nm (PLM: Fig. 3b, SAXS: Fig. 4a).These phases consist of distinct different PES-BA species of 3a.While a low molecular weight fraction builds the lamellar phase, the isotropic contains polymeric amphiphiles (Fig. 2d).
For ASA terminated PES macroscopic homogeneous and storage stable dispersions could be obtained down to w $ 0.3.Apart from peculiarities reecting an impact of the nature of the alkenyl side group all PES-BA 3c-3g show ordering somewhere within the parameter space dened by temperature (0-80 C) and composition (0.3 < w # 0.6) according to SAXS reections (listed, Table S2 †) and birefringence textures.Except for 3g these peaks shi to lower q values with decreasing concentration indicating larger distances or objects.Besides these reections humps appear which will be discussed below.
Thus PES-BA 3c (C8 chains) at w ¼ 0.33 shows increased ordering upon cooling from 20 C to 5 C where the hump transforms into a sharp peak (Fig. 4b) while the Schlieren-like birefringence, typical for nematic ordering, becomes more intense (Fig. 3c).Both vanish at higher temperatures, shown for SAXS at 70 C.A similar, less pronounced temperature dependence was found with PES-BA 3f (tetrapropenyl C12) at w ¼ 0.49 where the hump only appears upon cooling.With increasing concentration 3f forms batonnets as was shown with PLM in a contact preparation (Fig. 3d).The isomeric 3e (branched C12 chains) does not show any birefringence between 0.34 # w # 0.47 below 50 C.This might be due to homogeneous alignment as SAXS displays both types of reections.Above 50 C birefringence without texture of an ordered isotropic, e.g.bicontinuous, phase appears.At lower concentration (w ¼ 0.31, not shown) this was found within huge isolated domains of a non-birefringent liquid.
Increasing both the length and the non-uniformity of the aliphatic chains in 3g (branched C16 and C18 chains) leads to aqueous compositions (w ¼ 0.5-0.6)that show anisotropy in SAXS (Fig 5b).The birefringence texture of shear banded oily streaks persists up to 50 C and is evocative of a lamellar phase (Fig. 5a).However the correlation length is rather limited regarding missing harmonics.
In conjunction with the morphological variety of mesophases formed by bola-amphiphiles in binary systems with water over vast concentration and temperature ranges 8 the clarication of the self-assembly of PES-BA 3a to 3g during the manufacturing procedure remains a matter of further studies, e.g. in situ SAXS measurements.However with regard to reported bi-layered membrane formation in concentrated C16-ASA solution 19 and the shi from vesicle to disk formation in bolaamphiphile water systems with increasing temperature from 20 C to 60 C (ref.12) we assume that the PES-BA tend to adopt layered assemblies in the course of the dilution process.This is corroborated by our ndings on the dilution of succinic and maleic ester bearing PES-BA 3a and 3b resp.(vide supra), distinct peaks (1 st and 2 nd harmonic) in concentrated phases of TMA terminated PES-BA 3h (Table S2, † n.b.: double ionic strength) as well as indications for lamellae formed by ASA terminated PES-BA 3g.Contrary to the succinic (and maleic) terminated counterparts, the latter can be further diluted and does not phase separate.This suggests either the presence of less extended or porous lamellae and (or) intermolecular stabilization via hydrophobic attraction of the terminal hydrophobic side chains.
However in the case of ASA head groups that bear shorter (linear or branched) chain stabilization seems to favor nite anisotropic objects like rods, disks or ribbons that tend to nematic ordering (cf.3c, 3f).Indeed cryo-AFM imaging of PES-BA 3c (w ¼ 0.4) (Fig. 6) and 3f (w ¼ 0.49) (Fig. S1 †) suggests preferentially aligned anisotropic objects of dimensions and spacing that roughly correspond to observed humps and peaks in SAXS (appr.10-20 nm).
These humps might reect a 'correlation hole effect' rather than an ordering phenomenon: only by using a high ux X-ray source Cortese et al. observed broad reections in bolamaphile dispersions and attributed them to the distance between electron richer heads attached to the aliphatic spacer. 20However with regard to the esterication process of PES (vide supra) this interpretation would imply unlikely high proportions of high molecular weight species, e.g.2c capped HPA : DFA : HBA ¼ 3 : 3 : 7 (18.3nm, Table S1 †), that could cause humps around 20 nm.As their positions shi to lower q values with decreasing w values (Table S2 †) we assume that they reect disordered dispersion states of discrete objects consisting of assembled bola-amphiphiles.This is in line with the hump to peak transition in the scattering curves of 3c (w ¼ 0.33) during cooling (Fig. 4b).
Such objects may be formed by co-assembly of polymeric and diester based bola-amphiphiles (cf.composition of PES) while anisotropy arises from their different molecular dimensions of $5 to 10 nm versus $2 nm respectively (cf.Tables S1 and S2, † peak positions).There is a pronounced shi of the peaks around 7 nm to values around 10 nm with decreasing concentration for all ASA bearing systems.This shi is much less for higher w values of 3h.We interpret this by a variable degree of chain elongation of longer, exible amphiphiles, especially DFA comprising ones, in response to favorable charge separation as a function of the number of ionic groups (higher for 3h) and the degree of dissociation which should increase upon dilution.For the succinic ester and ASA based systems accommodation of oligomers with intermediate molecular weight and dimensions has to be considered and discussed in the context of lower degrees of carboxylic acid neutralization.Indeed considerable amounts of amine evaporated in the course of sample preparation (Table S2 †).Consequently, as a function of water content, varying amounts of non-neutralized acid groups of shorter bola-amphiphiles could be embedded within the hydrophobic core of the assemblies (Fig. 7).Such an accommodation seems reasonable in the light of similar ndings reported for glycerol terminated asymmetric bola-amphiphiles.The different behaviors of succinic and alkenyl succinic bearing amphiphiles reveal a twofold role of hydrophobic side chains neighboring the polar head group: (i) stabilization of assemblies of different species and (ii) packing frustration that impedes the formation of extended lamellae.
To summarize the results we have shown that ordered mesophases can be obtained from an ill-dened system of bolaamphiphiles comprising monomeric and polymeric species and that the presence and the nature of terminal hydrophobic side groups have a distinct impact on the assembly and thus the degree of order of the obtained dispersions.In the following chapter ordering phenomena of ASA based HBA are described in more detail.

Mesophases from monomeric diester bola-amphiphiles
Hydrogenated bisphenol-A alonedespite being rather bulky and hydrophobicapparently fails to provide a suitable hydrophobic spacer for the diester 4a (succinic) and 4h (trimellitic).The corresponding ammonium salts do not show any birefringence in contact preparations and in the bulk 4h quickly dissolves as a bola electrolyte (not shown).In order to shed light on the assembly of ASA terminated PES-BA, reference compounds 4c (linear C8), 4d (linear C12) and 4e (branched C12) have been prepared and their aqueous mesophases have been studied.Apart from their uniform molecular weights these compounds, like their polymeric counterparts, consist of molecular mixtures regarding isomerism of the diol, the alkenyl chain and its position relative to the succinic ester group (vide infra).
Although heating would be unnecessary with regard to low viscosities compared to the concentrated PES-BA, the neutralized diester was diluted with water at temperatures above 60 C. Thus homogeneous, transparent and storage stable dispersions were obtained either in the form of gels or viscous uids in the concentration range 0.3 # w # 0.7.In contrast, dilution of 4e at room temperature yields heterogeneous mixtures of a coarsely dispersed white waxy solid indicating metastable regions in the phase diagram of this system.
Structural analysis of the aqueous phases of 4c, 4d and 4e reveals a common feature in SAXS: two more or less pronounced peaks at positions in line with lamellae of decreased spacing with increasing bola-amphiphile contents as shown for 4c (Fig. 8).At the lowest concentration the main reex is rather broad and the second peak barely visible as a step while at w ¼ 0.4 faint peaks of inverted intensities are found (besides a weak 3 rd harmonic).For higher concentrations peak intensities rise.Similar sets of peaks are found for the mesophases of 4d and 4e (Fig. S3 and Table S4 †).However for the linear C12 chains these are only present at higher bola-amphiphile contents (w $ 0.6).Compared to the corresponding C8 based phase the repeat distance increases about 0.63 nm (w ¼ 0.7), which nearly matches twice the length of an all-trans C4 increment (0.312 nm).At lower concentrations broad humps indicate poor ordering.Intriguingly the branched C12 groups impede ordering at the highest concentration while the abnormal peak intensities described above for 4c (w ¼ 0.4) are shied to a concentration of w ¼ 0.5.Compared to the other amphiphiles at w ¼ 0.7 the differences of the peak (or hump) positions translate into roughly half the distance found for the comparison between 4c and 4d, specically 0.34 nm larger than in 4c and 0.29 nm smaller than in 4d (Table S4 †).This would be in line with a less extensive space lling of the branched chains in 4e.Although plausible with regard to molecular extensions the difference between repeat distances in 4c and 4d at high w values can hardly be explained by a typical bola-amphiphile assembly.Such lamellar stacking between an aligned hydrophobic spacer and water with the polar heads forming the interface implies that the alkenyl chains should oriented inbound rather than into the water phase.However a bilayer type assembly of molecules with their two hydrophobic side chains being oriented asymmetric and two elongated ones forming the hydrophobic core of a lamella could explain the ndings.
All phases at w ¼ 0.3 except for 4c (Fig. 9a) and those at higher w values which only show humps in SAXS do not display any birefringence.Remarkably those which cause faint and  unusual SAXS peak ratios exhibit distinct shear dependent birefringence with oily streaks transforming into a marble texture (4c w ¼ 0.4 Fig. 9b and c) or into the dominant Maltese crosses (4e w ¼ 0.5 Fig. 9d) respectively.Such behaviour is evocative of lamellar polymorphism observed in several bilayered assemblies of two-and multi-component aqueous detergent mixtures.Thus the evolving texture of 4e (w ¼ 0.5) 22 could be indicative for a transition of a lamellar phase (L a ) into a multi-lamellar vesicle (L a1 ) phase. 23Indeed a cryo-SEM image of this mesophase reveals a lamellar morphology comprising domains with a distinct curvature (Fig. S5 †).For the texture change of 4c (w ¼ 0.4) polymorphism between an isotropic sponge (L 3 ) and a lamellar (L a ) or mesh phase respectively is reasonable. 24The former is known to readily align under an external force into the latter, both sharing scale invariance 25 and form in concentrated regimes and persist into dilute regions. 26hese observations are corroborated by 2 H-NMR experiments on corresponding samples, dissolved in D 2 O on an equimolar basis with regard to the H 2 O based counterparts.The extent of the 2 H quadrupole splitting depends on the degree of anisotropy at the amphiphile water interface and thus indicates the symmetry of the mesophase as well as the sample heterogeneity in the case of coexisting mesophases of different space groups. 27Curves of the mesophases of 4c (Fig. 10) show biphasic samples for w ¼ 0.3 (main portion isotropic, compared with birefringence, Fig. 9a) and w ¼ 0.4 (a set of peaks and shoulders respectively).At higher concentrations the anisotropy of the interface and thus the splitting increase to values typical for lamellar interfaces (peak separation $1.700 Hz). 28However the smooth "lling" between the peaks indicates the presence of more isotropic parts in these interfaces.Similar behaviour is found for 4d and 4e (Fig. S4 †).Their less ordered phases (humps in SAXS curves, vide supra) do not show quadrupole splittings while for the ordered concentrated phases of 4d the splitting equals that of 4c.For the branched C12 bearing bola-amphiphile 4e maximum splitting is much less (644 Hz) compared to that of both other systems.
While the structural analysis of the ordered phases reveals isotropic features of a lamellar morphology rheological properties of these phases accentuate the isotropic aspect.Indeed amplitude sweeps reveal a distinct gel character of 4c over the whole concentration range with G 0 exceeding G 00 by factors of 6 to 15 and yield points between 5 and 45 Pa.kThis could be best described with a percolating, force transducing, bicontinuous morphology.The ordered phases of 4d (w $ 0.6) and of 4e (w < 0.6) show a similar behaviour while the less ordered phases (humps in SAXS) of both 4d (w < 0.6) and 4e (w > 0.6) are viscous uids.This is exemplary shown for the phases at w ¼ 0.5 (Fig. 11) and leaves 4e at w ¼ 0.6 as an exceptional dispersion where order and viscous behaviour coincide.
For all ordered dispersions of 4e (0.3 < w < 0.6) elasticity and viscosity considerably decline upon heating in the range of 35-60 C, with higher concentrated phases becoming uid more quickly (Fig. S6a †).This is accompanied by a loss of the anisotropic structure, revealed by quadrupole splitting (Fig. S6b †).Noteworthy inverse behaviour takes place with 4d at w ¼ 0.5.Being isotropic at room temperature, this system forms a coexisting anisotropic part upon heating (Fig. S7b †).This transforms the viscous liquid into an elastic dispersion at 50 C (Fig. S7a †).All dispersions of 4c and the ordered phases of 4d retain moduli at least up to 80 C or 90 C (4c w ¼ 0.5, 4d w $ 0.6) respectively (Fig. S8a and S7a †).For 4d (w ¼ 0.7) an isotropic fraction is formed around the yield point which dominates in the uid region (Fig. S7b †) while only slight  k Measured at 1 Hz.frequency sweeps of 4c and 4e (w ¼ 0.4) as well as 4d (w ¼ 0.5 and 0.6) measured with an amplitude of 0.1 mrad are shown in Fig. S12.Between 1 and 100 Hz moduli steadily increase, the loss moduli of the hydrogels approaching the storage moduli at the high frequency end.For the viscous liquid 4d (w ¼ 0.5) exponentially increasing G 0 narrows the gap between moduli at higher frequency.The typical range of shear stress covered by the sweeps was in the range of 0.4 and 18 Pa for the hydrogels and 10 to 24 Pa for the viscous liquid of 4d (w ¼ 0.5).changes in the quadrupole splitting of 4c based dispersions were observed until 80 C like the merger of the coexisting curvatures at w ¼ 0.4 (cf.Fig. 10) into one anisotropic phase around 60 C (Fig. S8b †).
To summarize the experimental results we have shown that HBA based bola-amphiphiles with C8-C12 ASA head groups self-assemble in water into anisotropic structures resembling mesh and coexistent bicontinuous phases.With regard to the terminal hydrophobic side group the linear C12 system fails to maintain ordered assemblies with increasing dilution whereas non-uniform, bulky groups of the branched C12 system impede ordering at high concentration and the formation of temperature stable structures in dilution.In contrast linear C8 groups allow for ordered structures over the entire range of covered concentrations and temperatures.
With regard to the polymorphism we suggest that molecular diversity of the diester bola-amphiphile most probably impedes the formation of different well-dened, e.g.lamellar assemblies.We calculated a possible composition of 4c (the same would apply to 4d) on the basis of analytical results regarding isomerism of educts and reaction products.As outlined in the ESI † HBA consists of ve species.Apart from two minor unassigned monools the diol (w ¼ 0.97) comprises a trans,trans (w ¼ 0.50), a cis,trans (w ¼ 0.42) and a cis,cis (w ¼ 0.08) isomer.ASA 2c consists of trans-and cis-congured double bonds in the unsaturated chain in a 4 : 1 ratio.Anhydride addition yields two different succinic ester groups with alkenyl chains either in the aor b-position (a/b ¼ 0.61).Lacking further information concerning symmetry with respect to isomeric head groups in 4c(d) we assume independent probabilities and ignore the monool fraction.Thus 35 different species in proportions between 21.73 and 0.97 mol% (Table S3 †) are obtained.On this basis it is reasonable that bola-amphiphiles of different shapes (e.g.cis,cis vs. trans,trans) and varying distances between the hydrophobic chains (e.g.a,avs.b,b) can be allocated to sites of different curvature in order to minimize packing frustration.Thus molecular diversity induces polymorphism of a few rather similar structures over a broad concentration range, contrary to molecular uniform systems that usually display morphological plurality with varying water content. 29Indeed molecular simulations of 4c in water in the range of 0.3 # w # 0.6 yield anisotropic morphologies like biased bicontinuous phases (shown for w ¼ 0.3, Fig. 12) and porous lamellae.It is noteworthy that although slight changes in isomer compositions can generate qualitative changes in the morphology, all of them resemble anisotropic meshes or bicontinuous phases.These results conrm the experimental ndings and support our interpretation.
Thermal stability as well as the order at very high concentration decreases in the case of branched side groups while   S3 †).Only the molecules of 4c and the ammonium ions are shown (red ¼ oxygen atoms) while the box is periodically repeated once in each direction (x, y, z) for clarity.
longer linear chains impede ordering at lower concentrations.The former is intuitively understood regarding the bulkiness of the hydrophobic moieties.For the longer chains most probably intermolecular hydrophobic attractions between just a fraction of differently shaped isomers induce rupture of the percolating assembly as it is evidenced by the uid nature of the more dilute dispersions.

Bola-amphiphiles, bola-electrolytes and non-bolaamphiphiles
As mentioned in the preceding chapter compounds 4a and 4h do not form ordered assemblies in water** as do the ASA based analogues.With respect to the latter we were interested in the question at which point with respect to the spacer length the ordering would be changed or impeded: where does the bola-amphiphile transform into a Gemini surfactant or a bolaelectrolyte respectively?We synthesized the diester from 1,4-bis(hydroxymethyl)cyclohexane (cis : trans ¼ 0.43) with 2a, 2c and 2e respectively in order to maintain the bulkiness and rigidity of HBA.However all dispersions of the neutralized diacids 5a, 5c and 5e are transparent low viscous uids that do not display any birefringence in OPM, contrary to the hydrolyzed and neutralized ASA 2c which forms well-dened mesophases in water (Fig. S9 †).Thus a shorter yet rigid spacer forces ASA based diester to dissolve as bola-electrolytes.
In the preceding section the pronounced tendency of 4c to form hydrogels of distinct temperature stability was shown, while PES-BA 3c assembles into nanosized, nematic aligned objects.We expected a pronounced gel character for dispersions of higher molecular weight polyester that comprise angular chains or a branched architecture like PES-NB, a chain extended PES via TMA (Fig. 1).The hydroxyl terminated polyester bears mid chain carboxylic acids.Dilution of the molten neutralized PES at 80 C yields homogeneous, translucent hydrogels in the concentration range 0.7 $ w $ 0.3.With w ¼ 0.35 a freshly prepared dispersion displays Schlieren texture of a nematic ordering which changes into featureless birefringence † † within a few days (Fig. 13).Cryo-TEM images, taken aer several weeks, reveal worm like patterns of truncated bicontinuous phases (Fig. 14).Aer 26 months under ambient conditions the gel still manifests its undulated surface (Fig. S10 †) and tangibly oscillates in response to manual knocking against the container.The distinct time dependency of the ordering that produces an elastic solid with a pronounced yield point prompted us to study the rheological development of the dispersion in the temperature range of 10 to 60 C. Heating causes an increase of the loss modulus (G 00 ) until 30 C followed by a decrease of both moduli until, at 60 C, G 00 dominates the storage modulus (G 0 ).Comparing a freshly prepared with an aged sample the gel uid transition starts on a much higher viscosity level in the latter (not shown).Upon cooling of the heated sample the material follows simple Arrhenius behavior down to 30 C. Below that temperature G 0 increases further while G 00 decreases, ending with an elastic solid at 10 C (Fig. S11 †).The pronounced inertia of PES-NB is reected by the slow development of the viscoelastic components over time at higher temperatures (Fig. 15).

Conclusions
The present study describes an economic and scalable approach to anisotropic liquids and hydrogels making use of a novel bola-amphiphile motif consisting of alkenyl succinic ester terminated hydrophobic di-and polyester based spacers.We have shown that a remarkable degree of order can be obtained from ill-dened mesogens which are based on technical grade raw materials consisting of numerous isomers as well as a plurality in molecular weights regarding polymer comprising reaction mixtures.With regard to the latter we have shown that terminal hydrophobic side groups stabilize assemblies formed from species of different molecular weight and size.Such anisotropic nanosized objects display nematic or lamellar like ordering depending on the length of the terminal side chains.
Monomeric diester bola-amphiphiles from C8 to C12 alkenyl succinic ester attached to hydrogenated bisphenol-A as a rigid spacer yield ordered dispersions.Such hydrogels form over the most extended concentration and temperature range in the case of C8 side chains.These phases can best be understood as polymorphism between porous lamellae (mesh) and a L 3 sponge phase.The plurality of isomeric species with different shape and aliphatic chain position allows for the allocation of different curvatures of the water organic interface, thus leading to scale invariance and nearly constant elastic response to external force.Longer as well as bulkier groups narrow the concentration range for the ordered phases and, in the case of branched chains, their temperature stability.
It will be the matter of further studies to unravel in more detail the mesogen behaviour of individual isomers of the ASA-HBA family with regard to the spacer as well as the different side groups.
Regarding both the economic and sustainable feedstock with respect to alkenyl succinic anhydrides and dimer fatty acid as well as the simple synthesis and manufacturing processes, and taking into account comparatively low amounts of polar groups, the authors anticipate that the presented polymer based approach will pave the way for ordered liquids as a formulation basis in the development of anisotropic materials for large scale applications like functional lms, coatings or adhesives.Thus it is conceivable to make use of surface induced ordering effectsbe it macroscopic or internal ones like layered nano particles, process related small shear ow or intrinsically replicating order phenomena in passing through the systems phase diagram in the course of water evaporation.
However careful validation of technical grade raw materials and possibly adaptation of process parameters in the course of the scale-up procedure are required for the sake of quality control.

Fig. 1
Fig. 1 Survey of synthesized polymeric bola-amphiphiles (3) based on the a,u-polyesterdiol PES (1), hydrogenated bisphenol-A derived diester bola-amphiphiles (4) and the non-bola-amphiphile PES-NB obtained from (1) in an addition-polycondensation process.Increment 'A' in (1) symbolizes a mixture of A1 and A2 (see Experimental part).For structures (3) and (4) with R1 s H and trimellitic head group asymmetric molecules represent isomerism with R1 in the aor b-position and the pristine CO 2 H group in the meta or para position respectively (see text).Replacement of HBA by the 1,4-bis(hydroxymethyl)cyclohexane (CHDM) corresponding diester has led to the synthesis of 2a, 2c and 2e respectively, that are omitted for clarity.

Fig. 5
Fig. 5 (a) Birefringence of 3g (w ¼ 0.5) at 40 C (scale bar 200 mm) and (b) 2D SAXS pattern of the same phase at 20 C, showing an oriented mesophase with respect to the peak positions at q ¼ 0.46 nm À1 .

Fig. 6
Fig. 6 Cryo-AFM images (topography left, phase contrast right) of 3c (w ¼ 0.4) reveal aligned domains with dimensions and spacings of the order of 10 to 20 nm respectively, resembling the nematic order.

Fig. 8
Fig.8SAXS curves from phases of 4c at 0.3 < w > 0.7 and 20 C. Peaks shift to higher q values with increasing w.

Fig. 12
Fig. 12 Simulated bicontinuous morphology of 4c in water (w ¼ 0.3) after 100 ns at 20 C. Box size 16.000 atoms comprising 34 molecules of 4c, taking into account the 15 most prevalent isomers (see text and TableS3†).Only the molecules of 4c and the ammonium ions are shown (red ¼ oxygen atoms) while the box is periodically repeated once in each direction (x, y, z) for clarity.

Fig. 15
Fig. 15 Oscillating shear rheology on PES-NB in water (w ¼ 0.35) at 40 C taken during the cooling phase of a preheated (60 C max.) sample.The evolution of loss (G 00 , circles) and storage (G 0 , crosses) modulus in the time domain was followed at two different shear stresses (10 Pa, blue and red and 100 Pa, green and black).

**
Missing birefringence, but dried 4a displays textures of crystals.† † Macroscopic the sample can easily be sliced into extended akes.